Electrophysiological techniques in marine microalgae study: A new perspective for harmful algal bloom (HAB) research.

Electrophysiological techniques, by measuring bioelectrical signals and ion channel activities in tissues and cells, are now widely utilized to study ion channel-related physiological functions and their underlying mechanisms. Electrophysiological techniques have been extensively employed in the investigation of animals, plants, and microorganisms; however, their application in marine algae lags behind that in other organisms. In this paper, we present an overview of current electrophysiological techniques applicable to algae while reviewing the historical usage of such techniques in this field. Furthermore, we explore the potential specific applications of electrophysiological technology in harmful algal bloom (HAB) research. The application prospects in the studies of stress tolerance, competitive advantage, nutrient absorption, toxin synthesis and secretion by HAB microalgae are discussed and anticipated herein with the aim of providing novel perspectives on HAB investigations.

Consumer acceptance of microalgae as a novel food - Where are we now? And how to get further.

Microalgae provide a potential new food resource for sustainable human nutrition. Many microalgae species can produce a high content of total protein with a balanced composition of essential amino acids, healthy oils rich in polyunsaturated fatty acids, carotenoids, fibers, and vitamins. These components can be made available via unprocessed microalgae or refined as individual ingredients. In either case, if added to foods, microalgae may affect taste, smell, texture, and appearance. This review focuses on how consumer acceptance of new foods - such as microalgae - can be accessed in the world of sensory science by bringing together examples from recent consumer surveys. The main aim is to obtain an overview of the attitude towards microalgae as a food ingredient in Europe. The overarching finding suggests that European consumers generally find microalgae acceptable as ingredients in food products. However, there is a prevailing preference for keeping inclusion levels low, primarily attributed to the vivid green color that algae impart to food items upon addition. Additionally, consumers tend to favor the taste of freshwater algae over marine species, often finding the latter's pronounced fishy flavor less appealing.

Regime shift in microalgal dynamics: Impact of water level changes on planktonic and benthic algal biomass.

Whole-lake microalgal biomass surveys were carried out in Lake Balaton to investigate the seasonal, spatial, and temporal changes of benthic algae, as well as to identify the drivers of the phytobenthos. Phytobenthos was controlled mainly by light: the highest benthic algal biomass was in the shallow littoral region characterized by large grain size (sand) with good light availability but lower nutrient content in the sediment. During the investigated period, phytoplankton biomass showed a significant decrease in almost the entire lake. At the same time, the biomass of benthic algae increased significantly in the eastern areas, increasing the contribution of total lake microalgae biomass (from 20 % to 27 %). Benthic algal biomass increase can be explained by the better light supply, owing to the artificially maintained high water level which greatly mitigates water mixing. The decrease in planktonic algal biomass could be attributed to increased zooplankton grazing, which is otherwise negatively affected by mixing. As a result of the high water level, the trophic structure of the lake has been rearranged in recent decades with a shift from the planktonic life form to the benthic one while the nutrient supply has largely remained unchanged.

A typical acidic extracellular polysaccharide alludes to algae-bacteria-collaboration in microalgal-bacterial symbiosis.

Microalgal-bacterial symbioses are prevalent in aquatic ecosystems and play a pivotal role in carbon sequestration, significantly contributing to global carbon cycling. The understanding of the contribution of exopolysaccharides (EPSs), a crucial carbon-based component, to the structural integrity of microalgal-bacterial symbioses remains insufficiently elucidated. To address this gap, our study aims to enhance our comprehension of the composition and primary structure of EPSs within a specific type of granular microalgal-bacterial symbiosis named microalgal-bacterial granular sludge (MBGS). Our investigation reveals that the acidic EPSs characteristic of this symbiosis have molecular weights ranging from several hundred thousand to over one million Daltons, including components like glucopyranose, galactopyranose, mannose, and rhamnose. Our elucidation of the backbone linkage of a representative exopolysaccharide revealed a →3)-ß-D-Galp-(1→4)-ß-D-Glcp-(1→ glycosidic linkage. This linear structure closely resembles bacterial xanthan, while the branched chain structure bears similarities to algal EPSs. Our findings highlight the collaborative synthesis of acidic EPSs by both microalgae and bacteria, emphasizing their joint contribution in the production of macromolecules within microalgal-bacterial symbiosis. This collaborative synthesis underscores the intricate molecular interactions contributing to the stability and function of these symbiotic relationships.

Short-term memory effects in the phototactic behavior of microalgae.

Phototaxis, the directed motion in response to a light stimulus, is crucial for motile microorganisms that rely on photosynthesis, such as the unicellular microalga Chlamydomonas reinhardtii. It is well known that microalgae adapt to ambient light stimuli. On time scales of several dozen minutes, when stimulated long enough, the response of the microalga evolves as if the light intensity were decreasing [A. Mayer, Chlamydomonas: Adaptation phenomena in phototaxis, Nature, 1968, 217(5131), 875-876]. Here, we show experimentally that microalgae also have a short-term memory, on the time scale of a couple of minutes, which is the opposite of adaptation. At these short time scales, when stimulated consecutively, the response of C. reinhardtii evolves as if the light intensity were increasing. Our experimental results are rationalized by the introduction of a simplified model of phototaxis. Memory comes from the interplay between an internal biochemical time scale and the time scale of the stimulus; as such, these memory effects are likely to be widespread in phototactic microorganisms.

A microfluidic microalgae detection system for cellular physiological response based on an object detection algorithm.

The composition of species and the physiological status of microalgal cells serve as significant indicators for monitoring marine environments. Symbiotic with corals, Symbiodiniaceae are more sensitive to the environmental response. However, current methods for evaluating microalgae tend to be population-based indicators that cannot be focused on single-cell level, ignoring potentially heterogeneous cells as well as cell state transitions. In this study, we proposed a microalgal cell detection method based on computer vision and microfluidics, which combined microscopic image processing, microfluidic chip and convolutional neural network to achieve label-free, sheathless, automated and high-throughput microalgae identification and cell state assessment. By optimizing the data import, training process and model architecture, we solved the problem of identifying tiny objects at the micron scale, and the optimized model was able to perform the tasks of cell multi-classification and physiological state assessment with more than 95% mean average precision. We discovered a novel transition state and explored the thermal sensitivity of three clades of Symbiodiniaceae, and discovered the phenomenon of cellular heat shock at high temperatures. The evolution of the physiological state of Symbiodiniaceae cells is very important for directional cell evolution and early warning of coral ecosystem health.

Unveiling the ecological resilience and industrial potential of Skeletonema marinoi through mixotrophic cultivation in Nordic winter condition.

Mixotrophy, the concurrent use of inorganic and organic carbon in the presence of light for microalgal growth, holds ecological and industrial significance. However, it is poorly explored in diatoms, especially in ecologically relevant species like Skeletonema marinoi. This study strategically employed mixotrophic metabolism to optimize the growth of a strain of Skeletonema marinoi (Sm142), which was found potentially important for biomass production on the west coast of Sweden in winter conditions. The aim of this study was to discern the most effective organic carbon sources by closely monitoring microalgal growth through the assessment of optical density, chlorophyll a fluorescence, and biomass concentration. The impact of various carbon sources on the physiology of Sm142 was investigated using photosynthetic and respiratory parameters. The findings revealed that glycerol exhibited the highest potential for enhancing the biomass concentration of Sm142 in a multi-cultivator under the specified experimental conditions, thanks to the increase in respiration activity. Furthermore, the stimulatory effect of glycerol was confirmed at a larger scale using environmental photobioreactors simulating the winter conditions on the west coast of Sweden; it was found comparable to the stimulation by CO2-enriched air versus normal air. These results were the first evidence of the ability of Skeletonema marinoi to perform mixotrophic metabolism during the winter and could explain the ecological success of this diatom on the Swedish west coast. These findings also highlight the importance of both organic and inorganic carbon sources for enhancing biomass productivity in harsh winter conditions.

Competence of algal consortia under municipal wastewater: remediation efficiency, photosynthetic performance, antioxidant defense mechanisms and biofuel production.

Utilizing monoalgal species for wastewater treatment is facing tremendous challenges owing to changing wastewater complexity in terms of physico-chemical characteristic, nutrient and metal concentration. The environmental conditions are also fluctuating therefore, the formation of robust system is of utmost importance for concomitant sustainable wastewater treatment and bioenergy production. In the present study, the tolerance and adaptability potential of algal consortia-1 (Chlorococcum humicola and Tetradesmus sp.) and consortia-2 (Chlorococcum humicola, Scenedesmus vacuolatus and Tetradesmus sp.) treated with municipal wastewater were examined under natural environmental conditions. The results exhibited that consortia-2 was more competent in recovering nitrate-nitrogen (82.92%), phosphorus (70.47%), and heavy metals (31-73.70%) from municipal wastewater (100%) than consortia-1. The results further depicted that total chlorophyll, carbohydrate, and protein content decreased significantly in wastewater-treated consortia-1 as compared to consortia-2. However, lipid content was increased by 4.01 and 1.17 folds in algal consortia-1 and consortia-2 compared to their respective controls. Moreover, absorption peak at 1740.6 cm-1 reflected higher biofuel-producing potential of consortia-1 as compared to consortia-2 as confirmed through FTIR spectroscopy. The results also revealed that consortia-2 showed the highest photosynthetic performance which was evident from the increment in the active photosystem-II reaction center (1.724 ± 0.068), quantum efficiency (0.633 ± 0.038), and performance index (3.752 ± 0.356). Further, a significant increase in photosynthetic parameters was observed in selected consortia at lag phase, while a noteworthy decline was observed at exponential and stationary phases in consortia-1 than consortia-2. The results also showed the maximum enhancement in ascorbic acid (2.43 folds), proline (3.34 folds), and cysteine (1.29 folds) in consortia-2, while SOD (1.75 folds), catalase (2.64 folds), and GR (1.19 folds) activity in consortia-1. Therefore, it can be concluded that due to remarkable flexibility and photosynthetic performance, consortia-2 could serve as a potential candidate for sustainable nutrient resource recovery and wastewater treatment, while consortia-1 for bio-fuel production in a natural environment. Thus, formation of algal consortia as the robust biosystem tolerates diverse environmental fluctuations together with wastewater complexity and ultimately can serve appropriate approach for environmental-friendly wastewater treatment and bioenergy production.

A review of emerging membrane-based microalgal-bacterial processes for wastewater treatment: Process configurations, biological and membrane performance, and perspectives.

Microalgal-bacterial (MB) consortia create an excellent eco-system for simultaneous COD/BOD and nutrients (N and P) removals in a single step with significant reduction in or complete elimination of aeration and carbonation in the biological wastewater treatment processes. The integration of membrane separation technology with the MB processes has created a new paradigm for research and development. This paper focuses on a comprehensive and critical literature review of recent advances in these emerging processes. Novel membrane process configurations and process conditions affecting the biological performance of these novel systems have been systematically reviewed and discussed. Membrane fouling issues and control of MB biofilm formation and thickness associated with these emerging suspended growth or immobilized biofilm processes are addressed and discussed. The research gaps, challenges, outlooks of these emerging processes are identified and discussed in-depth. The findings from the literature suggest that the membrane-based MB processes are advanced biotechnologies with a significant reduction in energy consumption and process simplification and high process efficiency that are not achievable with current technologies in wastewater treatment. There are endless opportunities for research and development of these novel and emerging membrane-based MB processes.

A systematic review of antibiotics and antibiotic resistance genes (ARGs) in mariculture wastewater: Antibiotics removal by microalgal-bacterial symbiotic system (MBSS), ARGs characterization on the metagenomic.

Antibiotic residues in mariculture wastewater seriously affect the aquatic environment. Antibiotic Resistance Genes (ARGs) produced under antibiotic stress flow through the environment and eventually enter the human body, seriously affecting human health. Microalgal-bacterial symbiotic system (MBSS) can remove antibiotics from mariculture and reduce the flow of ARGs into the environment. This review encapsulates the present scenario of mariculture wastewater, the removal mechanism of MBSS for antibiotics, and the biomolecular information under metagenomic assay. When confronted with antibiotics, there was a notable augmentation in the extracellular polymeric substances (EPS) content within MBSS, along with a concurrent elevation in the proportion of protein (PN) constituents within the EPS, which limits the entry of antibiotics into the cellular interior. Quorum sensing stimulates the microorganisms to produce biological responses (DNA synthesis - for adhesion) through signaling. Oxidative stress promotes gene expression (coupling, conjugation) to enhance horizontal gene transfer (HGT) in MBSS. The microbial community under metagenomic detection is dominated by aerobic bacteria in the bacterial-microalgal system. Compared to aerobic bacteria, anaerobic bacteria had the significant advantage of decreasing the distribution of ARGs. Overall, MBSS exhibits remarkable efficacy in mitigating the challenges posed by antibiotics and resistant genes from mariculture wastewater.

Physiological regulation of microalgae under cadmium stress and response mechanisms of time-series analysis using metabolomics.

The investigation of heavy metal wastewater treatment utilizing microalgae adsorption has been extensively demonstrated. However, the response mechanism based on metabolomics to analyze the time-series changes of microalgae under Cd stress has not been described in detail. In this study, SEM/TEM demonstrated that Cd accumulated on the cell surface of microalgae and was bioconcentrated in the cytoplasm, vesicles, and chloroplasts. Carbonyl/quinone/ketone/carboxyl groups (OCO), membrane polysaccharides (OH), and phospholipids (PO) were involved in the interaction of Cd ions, and the chlorophyll content underwent a process of decreasing in the early stage (1.62 mg/g at 48 h) and recovering to the normal level in the late stage, and the contents of MDA, GSH, and SOD were all increased (29.7 nmol/g, 0.23 mg/g, and 30.01 u/106 cells) and then gradually returned to the steady state. The results of EPS content and fluorescent labeling showed that Cd induced the overexpression and synthesis of extracellular polysaccharides and proteins, which is one of the defense mechanisms participating in the reduction of cellular damage by complexed Cd. Metabolomics results indicated that the malate synthesis pathway was activated after Cd-20 h, and the microalgal cells began to shift the metabolic pathway to storage lipid or polysaccharide biosynthesis. In the Calvin cycle, the expression of D-Sedoheptulose 7-phosphate in Cd-20 h_vs_ck and Cd-72 h_vs_Cd-20 h firstly declined and then increased, and the photosynthesis system was suppressed at the beginning, and then gradually returned to normal to maintain the successful development of the dark reaction. The results of time series analysis revealed that the response of microalgae to Cd was categorized into fast response and slow response to regulate cell adsorption and growth metabolism.

Advances in responses of microalgal-bacterial symbiosis to emerging pollutants in wastewater.

Nowadays, emerging pollutants are widely used and exist in wastewater, such as antibiotics, heavy metals, nanoparticle and microplastic. As a green alternative for wastewater treatment, microalgal-bacterial symbiosis has been aware of owning multiple merits of low energy consumption and little greenhouse gas emission. Thus, the responses of microalgal-bacterial symbiosis to emerging pollutants in wastewater treatment have become a hotspot in recent years. In this review paper, the removal performance of microalgal-bacterial symbiosis on organics, nitrogen and phosphorus in wastewater containing emerging pollutants has been summarized. The adaptation mechanisms of microalgal-bacterial symbiosis to emerging pollutants have been analyzed. It is found that antibiotics usually have hormesis effects on microalgal-bacterial symbiosis, and that microalgal-bacterial symbiosis appears to show more capacity to remove tetracycline and sulfamethoxazole, rather than oxytetracycline and enrofloxacin. Generally, microalgal-bacterial symbiosis can adapt to heavy metals at a concentration of less than 1 mg/L, but its capabilities to remove contaminants can be significantly affected at 10 mg/L heavy metals. Further research should focus on the influence of mixed emerging pollutants on microalgal-bacterial symbiosis, and the feasibility of using selected emerging pollutants (e.g., antibiotics) as a carbon source for microalgal-bacterial symbiosis should also be explored. This review is expected to deepen our understandings on emerging pollutants removal from wastewater by microalgal-bacterial symbiosis.

Development and Application of an Automated Raman Sensor for Bioprocess Monitoring: From the Laboratory to an Algae Production Platform.

Microalgae provide valuable bio-components with economic and environmental benefits. The monitoring of microalgal production is mostly performed using different sensors and analytical methods that, although very powerful, are limited to qualified users. This study proposes an automated Raman spectroscopy-based sensor for the online monitoring of microalgal production. For this purpose, an in situ system with a sampling station was made of a light-tight optical chamber connected to a Raman probe. Microalgal cultures were routed to this chamber by pipes connected to pumps and valves controlled and programmed by a computer. The developed approach was evaluated on Parachlorella kessleri under different culture conditions at a laboratory and an industrial algal platform. As a result, more than 4000 Raman spectra were generated and analysed by statistical methods. These spectra reflected the physiological state of the cells and demonstrate the ability of the developed sensor to monitor the physiology of microalgal cells and their intracellular molecules of interest in a complex production environment.

Heterogeneous aggregation between microplastics and microalgae: May provide new insights for microplastics removal.

Existing studies have shown that microplastics (MPs) as artificial surfaces can be colonized by plankton microorganisms. However, systematic research on exploring the aggregation formation process of MPs and microalgae is still lacking and particularly the influencing factors of aggregation remain to be elucidated. Therefore, this study investigated the heterogeneous aggregation process between various microalgal species (i.e., Chlorella vulgaris, Scenedesmus obliquus, Tetraselmis subcordiformis, Chaetoceros müelleri and Streptococcus westermani) and MPs (i.e., mPS and mPLA) with different sizes (i.e., 74 µm and 613 µm), concentrations (i.e., 0.1 g/L, 1 g/L and 2 g/L) and shapes (i.e., the particle and sheet). The results showed that microalgae can first attach to the holes or protrusions of MPs and highly accumulate in the local region, and then multi-layer aggregation can be formed subsequently. The aggregation degree between MPs and microalgae was closely related to the MPs shape and size, and was less related to the MPs concentration. The aggregation speed of small-sized MPs (e.g., 74 µm) was faster than the large-sized ones (e.g., 613 µm). The MPs in a shape of sheet were more obvious than those in particle on their aggregation with microalgae. The density of aggregates was increased compared with pristine MPs, which is related to the cell density and cell number of attached microalgae. For the same type of MPs, the aggregation degree for the tested microalgae was as follows: Scenedesmus obliquus > C. vulgaris > T. subcordiformis > C. müelleri > S. westermani. Meanwhile, MPs inhibited cell growth of microalgae, particularly under the circumstance of their aggregation, by limiting the gas and mass transfer between microalgal cells and the extracellular environment. The heterogeneous aggregation of MPs and microalgae may provide new ideas for treatment and controlling of MPs in the environment.

Biomass, photosynthetic activity, and biomolecule composition in Chlorella fusca (Chlorophyta) cultured in a raceway pond operated under greenhouse conditions.

Raceways are widely used as microalgae culture systems due to their low cost, but they are not the best option for biomass yield. Understanding in situ photosynthetic performance can be a first step to increase their biomass productivity. This study aimed at comparing the real time photosynthetic activity in a greenhouse raceway culture (250 L) with discrete measurements under laboratory conditions. We evaluated the photophysiology and biochemical composition of Chlorella fusca culture up to 120 h. In situ photosynthetic activity was continuously monitored and compared to discrete ex situ measurements; biochemical compounds were measured daily. The results showed a final biomass density of 0.45 g L-1 (5 days - 120 h) and an increase of the electron transport rate (ETR) up to 48 h but decreased thereafter. When the relative ETR was estimated considering the absorption coefficient (a) positive correlations of this parameter with photosynthetic capacity, cell density, biomass, biocompounds and antioxidant activity were obtained, whereas no correlation was detected without considering a. In situ photosynthesis monitoring showed higher absolute maximal ETR (10 - 160 µmol m-3s-1) than discrete ex situ measurements. We demonstrated the importance of considering the light absorption coefficient for expressing photosynthetic capacity and showed that C. fusca can produce, in the short-term, bioactive compounds that are correlated to photosynthetic conditions.

Thermal response analysis and compilation of cardinal temperatures for 424 strains of microalgae, cyanobacteria, diatoms and other species.

Microalgae and other phototrophic microorganisms can be cultivated to produce food and valuable bioproducts, also allowing to remove nutrients from wastewater and CO2 from biogas or polluted gas streams. Among other environmental and physico-chemical parameters, microalgal productivity is strongly influenced by the cultivation temperature. In this review, cardinal temperatures identifying the thermal response, i.e., the optimal growth condition (TOPT), and the lower and upper limits for microalgae cultivation (TMIN and TMAX), have been included in a structured and harmonized database. Literature data for 424 strains belonging to 148 genera of green algae, cyanobacteria, diatoms, and other phototrophs were tabulated and analysed, with a focus on the most relevant genera that are currently cultivated at the industrial scale in Europe. The dataset creation aimed at facilitating the comparison of different strain performances for different operational temperatures and assisting in the process of thermal and biological modelling, to reduce energy consumption and biomass production costs. A case study was presented, to illustrate the effect of temperature control on the energetic expenditure for cultivating different Chorella sp. strains under a greenhouse located in different European sites.

Microalgae and bio-polymeric adsorbents: an integrative approach giving new directions to wastewater treatment.

This review analyses the account of biological (microalgae) and synthetic (bio-polymeric adsorbents) elements to compass the treatment efficiencies of various water pollutants and mechanisms behind them. While considering pollutant removal, both techniques have their own merits and demerits. Microalgal-based methods have been dominantly used as a biological method for pollutant removal. The main limitations of microalgal methods are capacity, scale, dependence on variables of environment and duration of the process. Biopolymers on the other hand are naturally produced, abundant in nature, environmentally safe and biocompatible with cells and many times biodegradable. Algal immobilization in biopolymers has promoted the reuse of cells for further treatment and protected cells from toxic environment monitoring and controlling the external factors like pH, temperature and salinity can promote the removal process while working with the mentioned technologies. In this review, a mechanistic view of both these techniques along with integrated approaches emphasizing on their loopholes and possibilities of improvement in these techniques is represented. In addition to these, the review also discusses the post-treatment effect on algal cells which are specifically dependent on pollutant type and their concentration. All these insights will aid in developing integrated solutions to improve removal efficiencies in an environmentally safe and cost-effective manner.Novelty statement The main objective of this review is to thoroughly understand the role of micro-algal cells and synthetic adsorbents individually as well as their integrative effect in the removal of pollutants from wastewater. Many reviews have been published containing information related to either removal mechanism by algae or synthetic adsorbents. While in this review we have discussed the agents, algae and synthetic adsorbents along with their limitations and explained how these limitations can be overcome with the integration of both the moieties together in process of immobilization. We have covered both the analytical and mechanistic parts of these technologies. Along with this, the post-treatment effects on algae have been discussed which can give us a critical understanding of algal response to pollutants and by-products obtained after treatment. This review contains three different sections, their importance and also explained how these technologies can be improved in the future aspects.

Prediction of the impact induced by Cd in binary interactions with other divalent metals on wild-type and Cd-resistant strains of Dictyosphaerium chlorelloides.

The metals present in freshwater have a toxic profile with bioaccumulation and are biomagnified along the aquatic food chain. The metals induce high sensitivity in most aquatic organisms, while others, such as some microalgae species, evolve towards resistance. Therefore, this research predicted through the Combination Index method the binary interaction exposed to divalent metals by inhibiting population growth in a Cd-resistant strain (DcRCd100) compared to the wild-type strain (Dc1Mwt) of Dictyosphaerium chlorelloides and evaluate the specific resistance level obtained by DcRCd100 to Cd relative to other divalent metals.The results showed that DcRCd100 presents resistance compared to Dc1Mwt in individual exposure in the order of Fe2+ > Ni2+ > Cd2+ > Co2+ > Zn2+ > Cu2+ > Hg2+ with 50% inhibitory concentration at 72 h of exposure (IC50(72)) values 1253, 644.4, 423, 162.7, 141.3, 35.1, and 9.9 µM, respectively. It induces cross-resistance with high antagonistic rates (Combination Index (CI); CI > > 1) in the Cd/Zn and Cd/Cu. Cd/Ni, its initial response, is antagonistic, and it ends in an additive (CI = 1). DcRCd100 showed a lower resistance in Co, and Cd/Fe resistance was reduced individually. The interaction with Hg increased its resistance ten times more than individually.This research highlights the use of the CI as a highly efficient prediction method of the binary metal interactions in wild-type and Cd-resistant strains of D. chlorelloides. It may have the potential for metal accumulation, allowing the development of new methods of bioremediation of metals in effluents, and to monitor the concentration of metals in wastewater, its relative availability, transport, and mechanisms on resistant strains of microalgae.

Instability caused swimming of ferromagnetic filaments in pulsed field.

Magnetic filaments driven by external magnetic field are an interesting topic of research in-terms of the possible bio-medical applications. In this paper, we investigated the applicability of using ferromagnetic filaments as micro swimmers both experimentally and numerically. It was found that applying a pulse wave field profile with a duty cycle of 30[Formula: see text] induced experimentally observable swimming, which is similar to the breast stroke of micro algae. Good agreement with numerical simulations was found. Moreover, for stable continuous swimming, an initial filament shape is required to avoid transition to the structurally preferred non-swimming S-like mode.

Microalgae as biostimulants: a new approach in agriculture.

This review aims to elucidate the state of the art of microalgae-based biostimulants as a tool in agriculture by summarizing the biologically active compounds factors that influence the use of microalgae biostimulants and their application methods in the field. Additionally, we examined the factors that support the use of microalgal biostimulants to face abiotic and biotic stress in crop plants. The use of microalgae in crop production and the benefits of seed preparation, foliar application, soil drenching, and hydroponic treatments were discussed. Furthermore, the use of these biostimulants in crop plants and their multiple benefits such as, better rooting, higher crop, fruit yields, drought and salinity tolerance, photosynthetic activity and pathogen resistance was thoroughly presented. The present situation of microalgal biostimulants and their difficulties in the market was analyzed, as well as the perspectives of their use. However, data shows that microalgal derived biostimulants can be used as an alternative for the protection of crops and plant growth regulators and play a significant key role in increasing the levels of production, yield and health of crops. Special interest needs to focus on investigating more microalgae species and their biological active compound factors, due to the largely untapped field. Perspectives regarding future research lines and development priorities were included.