Antiproliferative and antiangiogenic effects of ammonium tetrathiomolybdate in a model of endometriosis.

AIMS: Copper (Cu) is involved in the endometriosis progression. Herein, an experimental endometriosis model was used to evaluate whether its chelation with ammonium tetrathiomolybdate (TM) affects the proliferation and angiogenesis in endometriotic-like lesions and the participation of oxidative stress in these processes. MAIN METHODS: Female C57BL/6 mice were divided into three groups: sham-operated mice, endometriosis-induced mice, and TM-treated endometriosis-induced mice. Each animal in the third group received 0.3 mg of TM/day in their drinking water from the postoperative 15th day. The samples were collected after one month of induced pathology. In peritoneal fluids, Cu and estradiol levels were determined by electrothermal atomic absorption spectrometry and electrochemiluminescence, respectively. Endometriotic-like lesions were processed for the analysis of cell proliferation by PCNA immunohistochemistry, the expression of angiogenic markers by RT-qPCR, the presence of endothelial cells by immunofluorescent staining, and oxidative stress applying spectrophotometric methods. KEY FINDINGS: TM treatment decreased Cu and estradiol levels, which were increased by this pathology. In lesions, TM induced: (a) a decrease in tissue weight and volume, (b) a decrease in PCNA-positive cells, (c) antiangiogenic effects by decreasing the number of blood vessels, the mRNA expression of fibroblast growth factor 2 (Fgf2) and platelet-derived growth factor subunit B (Pdgfb), and the presence of endothelial cells, (d) a decrease in antioxidant activity and an increase in lipid peroxidation. SIGNIFICANCE: TM is a highly effective antiproliferative and antiangiogenic agent, modulating oxidative imbalance in endometriosis. Its anti-endometriotic potential is an attractive feature of TM as a possible non-hormonal treatment.

Evaluation of the release of nickel and titanium under orthodontic treatment.

The metal alloys used in dentistry are made mainly of nickel (Ni), titanium (Ti), and other elements such as molybdenum (Mo), zirconium (Zr), iron (Fe), tin (Sn), chrome (Cr), carbon (C), copper (Cu) and niobium (Nb) which can release metal ions in unstable environments. The aim of this work was determine the salivary pH before and during orthodontic treatment; evaluate the release of metal ions, mainly Ni and Ti, in urine and saliva using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES); and evaluate the corrosion using Scanning Electronic Microscopy (SEM). In this study, we selected 35 individuals under orthodontic treatment, from whom saliva and urine samples were collected in 3 stages: (a) basal, (b) at 3 and (c) 6 months after the placement of the fixed appliances. SEM analyzed the Ni-Ti (0.016″) and stainless steel (SS) (0.016 × 0.022″) archs after 1 month of being in contact with the oral cavity. Statistical analysis was performed with Stata using the ANOVA model of repeated measures with a p < 0.05. A statistically significant difference in the concentration of Ni in saliva were found between 3 and 6 months of intervention and Ti in urine was found 3 and 6 months.

Alterações dentoalveolares em adultos promovidas pelo uso de arco auxiliar de expansão em TMA avaliadas por meio de tomografias computadorizadas / Dentoalveolar effects of using a TMA auxiliary expansion archwire: analysis of computed tomography

A atresia maxilar é um quadro de desarranjo de desenvolvimento do arco dentário presente em diversos tipos de má oclusão, desde as alterações transversais mais simples e puras até as formas mais graves, contemplando as divergências verticais e sagitais. O diagnóstico desta atresia, muitas vezes, é simplificado na presença ou não de mordida cruzada posterior, sendo ela unilateral ou bilateral. No entanto, faz-se necessário uma avaliação mais criteriosa que compreenda não só a própria alteração de forma do arco dentário, mas também as modificações oclusais subsequentes, como relação sagital de classe II ou III de Angle ou as discrepâncias verticais. Uma das possíveis formas de tratamento ortodôntico está ligada as expansões rápidas da maxila, método de incrementos ósseos por meio de forças ortopédicas, quando o indivíduo ainda apresenta potencial de crescimento craniofacial, ou seja, crianças e adolescentes. Já na vida adulta, esta possibilidade de ganhos ortopédicos não mais está presente e a opção não cirúrgica é o tratamento ortodôntico compensatório, por meio de expansão dentoalveolar do arco maxilar. O objetivo deste trabalho foi descrever os resultados de expansão dentoalveolar, obtidos utilizando-se o arco auxiliar de expansão em TMA (tungstênio, molibdênio e alloy), a partir de medidas lineares e angulares obtidas, bem como a integridade da cortical óssea vestibular desta área. Foi realizado um estudo retrospectivo de análise de tomografias computadorizadas, contidas na documentação ortodôntica de 13 pacientes tratados em uma clínica particular, realizadas antes e após a realização desta mecânica de expansão dentoalveolar. Para esta expansão, estes pacientes foram submetidos à instalação de um sobre-arco utilizado por vestibular como um arco auxiliar, sendo justaposto e unido ao fio de nivelamento principal (0,017x0,025 Termoativado) em cinco pontos, sendo 2 pontos nas entradas do tubo dos primeiros molares, 2 pontos entre os pré-molares e 1 ponto entre os incisivos centrais, por meio de fio de amarrilho 0,010 aço. Os resultados apresentaram ganhos estatisticamente significantes para aumento da distância das cúspides ao plano vertical mediano de todos os dentes medidos, bem como aumento da inclinação vestibular destes. A cortical óssea demonstrou adaptação, tendo deslocamento na mesma direção do movimento dentário, porém em menor quantidade. O aumento transversal das distâncias inter-dentárias também apresentou aumentos significativos e condizentes com a literatura. Desta forma, o arco auxiliar de expansão demonstrou-se eficiente para expansão dentoalveolar no paciente adulto, por meio de aumento da inclinação vestibular, com deslocamento dentário maior que o movimento de crista óssea, apresentado ganhos transversais significantes.(AU)

The Maxillary constriction is a developmental disorder present in various types of malocclusion, from the most simple and pure transverse changes to the most severe forms, causing vertical and sagittal problems. This malocclusion diagnosis is often simplified in the presence or not of posterior crossbite, which can be uni or bilateral. However, a complete evaluation must include not only the dental arch form changes, but also the subsequent occlusal modifications, such as sagittal relationships of Class II or III malocclusions and vertical discrepancies. Maxillary constriction treatment can be performed by rapid maxillary expansion, using orthopedic forces when there is still craniofacial growth. In adults, the possibility of orthopedic changes is no longer present and the non-surgical option is compensatory orthodontic treatment with dentoalveolar expansion, when the disorder magnitude allows. The objective of this study was to evaluate the effects of dentoalveolar expansion, obtained with a TMA (tungsten and molybdenum alloy) auxiliary expansion archwire, by means of linear and angular measurements, and the integrity of the buccal cortical bone in the posterior area. A retrospective analysis of CT scans, of orthodontic records of 13 patients treated at a private clinic, performed immediately before and after the auxiliary expansion archwire, was used. For the expansion, the patients underwent installation of a secondary arch combined with the primary archwire (0.017x0.025-inch heatactivated Ni-Ti), ligated in five points. Two points in the first molar tube entries, 2 points between the premolars and 1 point between the central incisors, with a 0.010- inch steel ligature wire. The results showed statistically significant transverse increase and buccal inclination for all teeth. The cortical bone showed adaptability and displacement in the same direction of tooth movement, but in smaller amounts. Thus, the auxiliary expansion arch wire proved to be effective to correct dentoalveolar constriction in adult patients, by increasing the buccal dental inclination with larger displacements than the bone crest adaptation and with significant transverse gains.(AU)

Effects of high molybdenum intake on 1.2 - dimethylhydrazine-induced intestinal tumors in rats

Wistar male rats, 3 months of age were given ad-libitum a nutritionally adequate diet and demineralized drinking water. The Molybdenum (Mo) and Tungsten (W) were provided in the drinking water at 200 ppm concentration. Intestinal tumors were induced by 1,2-dimethylhydrazine (DMH) given subcutaneously as 16 weekly doses at 20 mg/kg body weight. Mo in the form of (NH4)6 Mo7O24 4H2O or W in the form of (Na2WO4) were provided in the drinking water two months before the first DMH treatment and were continued during 4 months more until the last DMH treatment. Three months after the last carcinogen injection, all animals were sacrificed and examined for intestinal tumors. The number, size and location of the tumors were recorded and the pathology was examined. The addition of Mo to the drinking water induced an increase of hepatic Mo content. At the end of the second month, the hepatic content of Mo was 5.61 ppm, compared with control and W groups (2.18 and 0.96 ppm, respectively). A significantly lower incidence of tumors was observed in the Mo group (47), compared with the control group given DMH alone (105) and W group (113). On the other hand, the Mo group showed a significant decrease in the numbers of multiple tumors per rat. (AU)

Effects of high molybdenum intake on 1.2 - dimethylhydrazine-induced intestinal tumors in rats

Wistar male rats, 3 months of age were given ad-libitum a nutritionally adequate diet and demineralized drinking water. The Molybdenum (Mo) and Tungsten (W) were provided in the drinking water at 200 ppm concentration. Intestinal tumors were induced by 1,2-dimethylhydrazine (DMH) given subcutaneously as 16 weekly doses at 20 mg/kg body weight. Mo in the form of (NH4)6 Mo7O24 4H2O or W in the form of (Na2WO4) were provided in the drinking water two months before the first DMH treatment and were continued during 4 months more until the last DMH treatment. Three months after the last carcinogen injection, all animals were sacrificed and examined for intestinal tumors. The number, size and location of the tumors were recorded and the pathology was examined. The addition of Mo to the drinking water induced an increase of hepatic Mo content. At the end of the second month, the hepatic content of Mo was 5.61 ppm, compared with control and W groups (2.18 and 0.96 ppm, respectively). A significantly lower incidence of tumors was observed in the Mo group (47), compared with the control group given DMH alone (105) and W group (113). On the other hand, the Mo group showed a significant decrease in the numbers of multiple tumors per rat.

Estudio histológico de los tejidos peri - implantarios: implantes yuxtaóseos en cromo - cobalto - molibdeno quirúrgico

Se describen los hallazgos histológicos de los tejidos periimplantarios en un caso de implante yuxtaóseo en el sector posterior de la cavidad bucal. La movilidad por enfermedad periodontal de los elementos dentarios 33 y 43, determinó el retiro de estos implantes que en su estudio clínico y radiográfico no presentaban características anormales. Mediante intervención quirúrgica se extrajo la estructura metálica acompañada por los tejidos blandos que la rodeaban en un solo block, procurando realizar todas las maniobras con un mínimo de traumatismo operatorio. Fijación formol al 10 por ciento. Impregnación en block con carbonato de plata amoniacal de Río Ortega; inclusión en metacrilato de metilo y cortes seriados con micrótomo de sierra LEITZ 1600. Los cortes con la relación metal-tejidos blandos, se analizaron en lupa estereoscópica con graduaciones entre 2 y 15,75 aumentos reales. La relación mucosa metal, constituye una hendidura gingivo metálica. El margen gingival esta recubierto por un epitelio pavimentoso que en la profundidad llega hasta un tejido conjuntivo fibroso parcialmente hialinizado que envuelve a las estructuras metálicas. No se observan procesos inflamatorios. Se plantea la posibilidad de aplicar esta metodología a los implantes osteointegrados para realizar el estudio de la interfase implante tejido óseo

Estudio histológico de los tejidos peri - implantarios: implantes yuxtaóseos en cromo - cobalto - molibdeno quirúrgico

Se describen los hallazgos histológicos de los tejidos periimplantarios en un caso de implante yuxtaóseo en el sector posterior de la cavidad bucal. La movilidad por enfermedad periodontal de los elementos dentarios 33 y 43, determinó el retiro de estos implantes que en su estudio clínico y radiográfico no presentaban características anormales. Mediante intervención quirúrgica se extrajo la estructura metálica acompañada por los tejidos blandos que la rodeaban en un solo block, procurando realizar todas las maniobras con un mínimo de traumatismo operatorio. Fijación formol al 10 por ciento. Impregnación en block con carbonato de plata amoniacal de Río Ortega; inclusión en metacrilato de metilo y cortes seriados con micrótomo de sierra LEITZ 1600. Los cortes con la relación metal-tejidos blandos, se analizaron en lupa estereoscópica con graduaciones entre 2 y 15,75 aumentos reales. La relación mucosa metal, constituye una hendidura gingivo metálica. El margen gingival esta recubierto por un epitelio pavimentoso que en la profundidad llega hasta un tejido conjuntivo fibroso parcialmente hialinizado que envuelve a las estructuras metálicas. No se observan procesos inflamatorios. Se plantea la posibilidad de aplicar esta metodología a los implantes osteointegrados para realizar el estudio de la interfase implante tejido óseo (AU)