RESUMEN
From pertussis to meningococcal disease and back represents nearly 30 years of research at Porton, first at the Centre for Applied Microbiology and Research and latterly as part of the Health Protection Agency. I joined the group lead by Andy Robinson developing an acellular pertussis vaccine and was part of an exciting period that encompassed basic antigen characterisation and pathogenesis studies with the development of an acellular vaccine containing fimbriae. Research then changed to focus on serogroup B meningococcal disease, studying the vaccine potential of iron-regulated proteins and then Neisseria lactamica. The resurgence of pertussis seen in some countries alerted me to the lack of understanding of protective immune responses to Bordetella pertussis infection and disease and this is now an active area of research.
Asunto(s)
Meningitis Meningocócica/epidemiología , Meningitis Meningocócica/prevención & control , Vacunas Meningococicas/inmunología , Vacuna contra la Tos Ferina/inmunología , Tos Ferina/epidemiología , Tos Ferina/prevención & control , Bordetella pertussis/genética , Bordetella pertussis/inmunología , Bordetella pertussis/patogenicidad , Historia del Siglo XX , Historia del Siglo XXI , Humanos , Meningitis Meningocócica/inmunología , Neisseria lactamica/genética , Neisseria lactamica/inmunología , Neisseria lactamica/patogenicidad , Tos Ferina/inmunologíaRESUMEN
Measuring cell proliferation and cell death during bacterial infection involves performing end-point assays that represent the response at a single time point. A new technology from Roche Applied Science and ACEA Biosciences allows continuous monitoring of cells in real-time using specialized cell culture microplates containing micro-electrodes. The xCELLigence system enables continuous measurement and quantification of cell adhesion, proliferation, spreading, cell death and detachment, thus creating a picture of cell function during bacterial infection. Furthermore, lag and log phases can be determined to estimate optimal times to infect cells. In this study we used this system to provide valuable insights into cell function in response to several virulence factors of the meningitis causing pathogen Neisseria meningitidis, including the lipopolysaccharide (LPS), the polysaccharide capsule and the outer membrane protein Opc. We observed that prolonged time of infection with pathogenic Neisseria strains led to morphological changes including cell rounding and loss of cell-cell contact, thus resulting in changed electrical impedance as monitored in real-time. Furthermore, cell function in response to 14 strains of apathogenic Neisseria spp. (N. lactamica and N. mucosa) was analyzed. In contrast, infection with apathogenic N. lactamica isolates did not change electrical impedance monitored for 48 h. Together our data show that this system can be used as a rapid monitoring tool for cellular function in response to bacterial infection and combines high data acquisition rates with ease of handling.
Asunto(s)
Células Endoteliales/efectos de los fármacos , Células Endoteliales/fisiología , Neisseria meningitidis/patogenicidad , Factores de Virulencia/toxicidad , Proteínas de la Membrana Bacteriana Externa/toxicidad , Células Cultivadas , Impedancia Eléctrica , Células Epiteliales/efectos de los fármacos , Células Epiteliales/fisiología , Fibroblastos/efectos de los fármacos , Fibroblastos/fisiología , Humanos , Lipopolisacáridos/toxicidad , Neisseria lactamica/patogenicidad , Neisseria mucosa/patogenicidad , Polisacáridos Bacterianos/toxicidadRESUMEN
BACKGROUND: The genus Neisseria contains two important yet very different pathogens, N. meningitidis and N. gonorrhoeae, in addition to non-pathogenic species, of which N. lactamica is the best characterized. Genomic comparisons of these three bacteria will provide insights into the mechanisms and evolution of pathogenesis in this group of organisms, which are applicable to understanding these processes more generally. RESULTS: Non-pathogenic N. lactamica exhibits very similar population structure and levels of diversity to the meningococcus, whilst gonococci are essentially recent descendents of a single clone. All three species share a common core gene set estimated to comprise around 1190 CDSs, corresponding to about 60% of the genome. However, some of the nucleotide sequence diversity within this core genome is particular to each group, indicating that cross-species recombination is rare in this shared core gene set. Other than the meningococcal cps region, which encodes the polysaccharide capsule, relatively few members of the large accessory gene pool are exclusive to one species group, and cross-species recombination within this accessory genome is frequent. CONCLUSION: The three Neisseria species groups represent coherent biological and genetic groupings which appear to be maintained by low rates of inter-species horizontal genetic exchange within the core genome. There is extensive evidence for exchange among positively selected genes and the accessory genome and some evidence of hitch-hiking of housekeeping genes with other loci. It is not possible to define a 'pathogenome' for this group of organisms and the disease causing phenotypes are therefore likely to be complex, polygenic, and different among the various disease-associated phenotypes observed.
Asunto(s)
Evolución Molecular , Genes Bacterianos/genética , Neisseria lactamica/genética , Neisseria lactamica/aislamiento & purificación , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Composición de Base/genética , Secuencia de Bases , Orden Génico/genética , Datos de Secuencia Molecular , Neisseria lactamica/patogenicidad , Filogenia , Homología de Secuencia de Ácido Nucleico , Virulencia/genéticaRESUMEN
Commensal bacteria comprise a large part of the microbial world, playing important roles in human development, health and disease. However, little is known about the genomic content of commensals or how related they are to their pathogenic counterparts. The genus Neisseria, containing both commensal and pathogenic species, provides an excellent opportunity to study these issues. We undertook a comprehensive sequencing and analysis of human commensal and pathogenic Neisseria genomes. Commensals have an extensive repertoire of virulence alleles, a large fraction of which has been exchanged among Neisseria species. Commensals also have the genetic capacity to donate DNA to, and take up DNA from, other Neisseria. Our findings strongly suggest that commensal Neisseria serve as reservoirs of virulence alleles, and that they engage extensively in genetic exchange.
Asunto(s)
Transferencia de Gen Horizontal/genética , Genoma Bacteriano/genética , Neisseria/genética , Virulencia/genética , Humanos , Neisseria/patogenicidad , Neisseria gonorrhoeae/genética , Neisseria gonorrhoeae/patogenicidad , Neisseria lactamica/genética , Neisseria lactamica/patogenicidad , Neisseria meningitidis/genética , Neisseria meningitidis/patogenicidadRESUMEN
The distribution of the hemoglobin receptor gene (hmbR) was investigated among disease and carriage Neisseria meningitidis isolates, revealing that the gene was detected at a significantly higher frequency among disease isolates than among carriage isolates. In isolates without hmbR, the locus was occupied by the cassettes exl2 or exl3 or by a "pseudo hmbR" gene, designated exl4. The hmbR locus exhibited characteristics of a pathogenicity island in published genomes of N. meningitidis, Neisseria gonorrhoeae, and Neisseria lactamica sequence type-640. These data are consistent with a role for the hmbR gene in meningococcal disease.
Asunto(s)
Proteínas Bacterianas/genética , Infecciones Meningocócicas/genética , Neisseria gonorrhoeae/genética , Neisseria meningitidis/genética , Receptores de Superficie Celular/genética , Proteínas Bacterianas/fisiología , Cartilla de ADN , Humanos , Infecciones Meningocócicas/inmunología , Neisseria lactamica/genética , Neisseria lactamica/patogenicidad , Neisseria meningitidis/patogenicidad , Receptores de Superficie Celular/fisiología , Virulencia/genéticaRESUMEN
Advances in high-throughput nucleotide sequencing and bioinformatics make the study of genomes at the population level feasible. Preliminary population genomic studies have explored the relationships among three closely related bacteria, Neisseria meningitidis, Neisseria gonorrhoeae and Neisseria lactamica, which exhibit very different phenotypes with respect to human colonisation. The data obtained have been especially valuable in the establishing of the role of horizontal genetic exchange in bacterial speciation and shaping population structure. In the meningococcus, they have been used to define invasive genetic types, search for virulence factors and potential vaccine components and investigate the effects of vaccines on population structure. These are generic approaches and their application to the Neisseria provides a foretaste for their application to the wider bacterial world.
Asunto(s)
Variación Genética , Neisseria gonorrhoeae/genética , Neisseria gonorrhoeae/patogenicidad , Neisseria lactamica/genética , Neisseria lactamica/patogenicidad , Neisseria meningitidis/genética , Neisseria meningitidis/patogenicidad , Factores de Virulencia/biosíntesis , Secuencia de Bases , Evolución Molecular , Transferencia de Gen Horizontal , Genoma Bacteriano , Genómica , Humanos , Recombinación Genética , Factores de Virulencia/genéticaRESUMEN
Anomalous DNA (aDNA) in prokaryotic genomes, identified by its aberrant nucleotide composition, generally represents horizontally acquired DNA. Previous studies showed that frequent DNA transfer occurs between commensal Neisseriae and Neisseria meningitidis. Currently, it is unknown whether aDNA regions are also transferred between these species. The genome of Neisseria lactamica strain 892586 was assessed by a strategy that enables the selective isolation of aDNA, using endonucleases with recognition sites that are overrepresented in aDNA. Of eight regions with aDNA, five displayed similarity to virulence-associated meningococcal sequences. Of three aDNA fragments with limited or no similarity to neisserial sequences, one encodes a novel putative autotransporter/adhesin. The remaining two fragments are adjacent in the N. lactamica genome, and encode a novel putative ATPase/subtilisin-like protease operon. A similar operon is present in the genomes of different respiratory tract pathogens. The identification of aDNA from N. lactamica with similarity to meningococcal aDNA shows that genetic exchange between the Neisseriae is not limited to the neisserial core genome. The discovery of aDNA in N. lactamica similar to a locus in other pathogens substantially expands the neisserial gene pool.
Asunto(s)
ADN Bacteriano/genética , Transferencia de Gen Horizontal , Neisseria lactamica/genética , Adenosina Trifosfatasas/genética , Adhesinas Bacterianas/genética , Secuencia de Aminoácidos , Proteínas Portadoras/genética , Biología Computacional , ADN Bacteriano/química , Genoma Bacteriano , Datos de Secuencia Molecular , Neisseria lactamica/patogenicidad , Neisseria meningitidis/genética , Operón/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Serina Endopeptidasas/genética , Virulencia/genéticaRESUMEN
Neisseria gonorrhoeae is a human pathogen causing the sexually transmitted disease gonorrhoeae. The bacteria preferentially attach to and invade epithelial cells of the genital tract. As these cells previously have been shown to express the human cathelicidin LL-37, we wanted to investigate the role of LL-37 during N. gonorrhoeae infection. The cervical epithelial cell line ME180 was utilized and the expression of LL-37 was confirmed on both peptide and transcriptional levels. Moreover, LL-37 exhibited potent in vitro activity against N. gonorrhoeae. Interestingly, the transcript and peptide levels of LL-37 were downregulated during infection, according to quantitative real-time polymerase chain reaction (PCR) and immunocyto-chemistry. The downregulation was most prominent with pathogenic strains of Neisseria, while non-pathogenic strains such as Neisseria lactamica and Escherichia coli only exhibited moderate effects. Heat-killed N. gonorrhoeae had no impact on the downregulation, emphasizing the importance of live bacteria. The results in this study suggest that pathogenic Neisseria may gain a survival advantage in the female genital tract by downregulating LL-37 expression.