RESUMEN
Sodium nitrite (NaNO2 ) is widely used in the food industry as a preservative and colorant in meat and fish products. Industrialization and improper agricultural practices have greatly increased human exposure to high nitrite levels, mainly through contaminated drinking water, causing various health disorders. We have investigated the protective effect of carnosine (CAR) and N-acetyl cysteine (NAC) on NaNO2 -induced toxicity in rat blood. CAR is a bioactive dipeptide found in mammalian muscle while NAC is a synthetic sulfhydryl amino acid and an important precursor of glutathione. Animals were given a single acute oral dose of NaNO2 at 60 mg/kg body weight with or without prior administration of either CAR or NAC. Rats were sacrificed after 24 h, blood was withdrawn and plasma and erythrocytes were isolated. Administration of NaNO2 alone increased methemoglobin levels and methemoglobin reductase activity, decreased the activities of antioxidant defense and metabolic enzymes and significantly weakened the total antioxidant capacity of rat erythrocytes. Similar effects were seen in plasma of NaNO2 -treated rats. In contrast, administration of CAR or NAC, prior to NaNO2 treatment, markedly attenuated the NaNO2 -elicited deleterious effects. Thus, CAR and NAC can mitigate nitrite-induced metabolic alterations and oxidative damage probably due to their intrinsic biochemical antioxidant properties. This study suggests that CAR and NAC can be potentially used as therapeutic/protective agents against NaNO2 toxicity.
Asunto(s)
Acetilcisteína/farmacología , Carnosina/farmacología , Estrés Oxidativo/efectos de los fármacos , Nitrito de Sodio/toxicidad , Animales , Antioxidantes/farmacología , Citocromo-B(5) Reductasa/sangre , Interacciones Farmacológicas , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Masculino , Metahemoglobina/metabolismo , Oxidación-Reducción , Sustancias Protectoras/farmacología , Ratas , Ratas Wistar , Nitrito de Sodio/antagonistas & inhibidores , Nitrito de Sodio/sangreRESUMEN
Nitrite is present as a noxious contaminant in drinking water and causes oxidative damage in various tissues of humans and animals. It is a well-known methemoglobin-forming agent that has been shown to damage blood cells. The protective effect of taurine, a semi-essential sulfur-containing amino acid, was studied on sodium nitrite (NaNO2)-induced oxidative damage in human erythrocytes. Erythrocytes were incubated with NaNO2, in the presence and absence of taurine, and changes in oxidative stress parameters determined. Pretreatment with taurine significantly ameliorated NaNO2-induced oxidative damage to lipids, proteins, and plasma membrane. It also reduced the NaNO2-induced increase in methemoglobin levels and ROS production. Taurine improved the antioxidant capacity of cells, restored the alterations in the activities of various metabolic enzymes, and prevented morphological changes in erythrocytes. Thus, taurine can be potentially used as a protective agent against the damaging effects of nitrite.
Asunto(s)
Eritrocitos/efectos de los fármacos , Metahemoglobina/metabolismo , Sustancias Protectoras/farmacología , Nitrito de Sodio/antagonistas & inhibidores , Taurina/farmacología , Animales , Antioxidantes/metabolismo , Eritrocitos/metabolismo , Humanos , Masculino , Oxidación-Reducción , Estrés Oxidativo/efectos de los fármacos , Nitrito de Sodio/toxicidadRESUMEN
Although sodium nitrite has been widely used as food preservative, building bases of scientific evidence about nitrite continues to oppose the general safety in human health. Moreover, thymoquinone (TQ) has therapeutic potential as antioxidant, anti-inflammatory, antibacterial and anticancer. Therefore, we investigated the effects of both sodium nitrite and TQ on testicular tissues of rats. Forty adult male Sprague Dawley rats were used. They received either 80 mg kg(-1) sodium nitrite or 50 mg kg(-1) TQ daily for twelve weeks. Serum testosterone was measured. Testis were weighed and the testicular tissue homogenates were used for measurements of tumour necrosis factor (TNF)-α, interleukin (IL)-1ß, IL-4, IL-6, IL10, caspase-3, caspase-8 and caspase-9. Sodium nitrite resulted in significant reduction in serum testosterone concentration and elevation in testis weight and Gonado-Somatic Index. We found significant reduction in testicular tissues levels of IL-4 and IL-10 associated with elevated levels of TNF-α, IL-1ß, IL-6, caspase-3, caspase-8 and caspase-9. In conclusion, chronic oral sodium nitrite induced changes in the weight of rat testis accompanied by elevation in the testicular tissue level of oxidative stress markers and inflammatory cytokines. TQ attenuated sodium nitrite-induced testicular tissue damage through blocking oxidative stress, restoration of normal inflammatory cytokines balance and blocking of apoptosis.
Asunto(s)
Benzoquinonas/farmacología , Nitrito de Sodio/antagonistas & inhibidores , Nitrito de Sodio/toxicidad , Testículo/efectos de los fármacos , Testículo/patología , Administración Oral , Animales , Apoptosis/efectos de los fármacos , Citocinas/metabolismo , Humanos , Mediadores de Inflamación/metabolismo , Masculino , Orquitis/inducido químicamente , Orquitis/fisiopatología , Orquitis/prevención & control , Tamaño de los Órganos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Nitrito de Sodio/administración & dosificación , Testículo/fisiopatología , Testosterona/sangreRESUMEN
AIMS: Sodium nitrite, a preservative used in meat products, helps in the production of free radicals, leading to increased lipid peroxidation, which plays a vital role in posing toxic effects in different body organs. On the other hand, arjunolic acid possesses antioxidant properties and plays protective roles against chemically induced organ pathophysiology. We investigated the effect of sodium nitrite on cardiac tissue in rats on the inflammatory cytokine balance and the type of induced apoptosis, and we analyzed the protective role of arjunolic acid. MAIN METHODS: Sixty adult male Sprague-Dawley rats were injected with 80mg/kg sodium nitrite in the presence/absence of arjunolic acid (100 and 200mg/kg). Cardiac pro-inflammatory cytokines (TNF-α and IL-1ß), c-reactive protein (CRP) and anti-inflammatory cytokines (IL-4 and IL-10) were measured by ELISA. Cardiac mitochondrial activity (cytochrome-C-oxidase), JNK activation and apoptosis (caspase-3, caspase-8 and caspase-9) were assessed. KEY FINDINGS: Sodium nitrite resulted in increased TNF-α (1.6-fold), IL-1ß (3.7-fold) and CRP (2.4-fold) levels accompanied by 52%, 59% and 40% reductions in IL-10, IL-4 and cytochrome-C-oxidase, respectively, as well as enhanced JNK, caspase-3, caspase-8 and caspase-9 activities. Arjunolic acid markedly ameliorated these effects. SIGNIFICANCE: Arjunolic acid attenuated sodium nitrite-induced cardiac damage in rats and restored the normal balance between pro- and anti-inflammatory cytokines. Moreover, arjunolic acid protected cardiac tissues from both extrinsic and intrinsic cell death pathways.
Asunto(s)
Apoptosis/efectos de los fármacos , Citocinas/análisis , Corazón/efectos de los fármacos , Miocardio/química , Nitrito de Sodio/antagonistas & inhibidores , Triterpenos/farmacología , Administración Oral , Animales , Proteína C-Reactiva/análisis , Complejo IV de Transporte de Electrones/efectos de los fármacos , Complejo IV de Transporte de Electrones/metabolismo , Interleucina-10/análisis , Interleucina-1beta/análisis , Interleucina-4/análisis , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Masculino , Mitocondrias Cardíacas/efectos de los fármacos , Mitocondrias Cardíacas/metabolismo , Ratas , Ratas Sprague-Dawley , Nitrito de Sodio/administración & dosificación , Nitrito de Sodio/toxicidad , Factor de Necrosis Tumoral alfa/análisisRESUMEN
The new pathway nitrate-nitrite-nitric oxide (NO) has emerged as a physiological alternative to the classical enzymatic pathway for NO formation from l-arginine. Nitrate is converted to nitrite by commensal bacteria in the oral cavity and the nitrite formed is then swallowed and reduced to NO under the acidic conditions of the stomach. In this study, we tested the hypothesis that increases in gastric pH caused by omeprazole could decrease the hypotensive effect of oral sodium nitrite. We assessed the effects of omeprazole treatment on the acute hypotensive effects produced by sodium nitrite in normotensive and L-NAME-hypertensive free-moving rats. In addition, we assessed the changes in gastric pH and plasma levels of nitrite, NO(x) (nitrate+nitrite), and S-nitrosothiols caused by treatments. We found that the increases in gastric pH induced by omeprazole significantly reduced the hypotensive effects of sodium nitrite in both normotensive and L-NAME-hypertensive rats. This effect of omeprazole was associated with no significant differences in plasma nitrite, NO(x), or S-nitrosothiol levels. Our results suggest that part of the hypotensive effects of oral sodium nitrite may be due to its conversion to NO in the acidified environment of the stomach. The increase in gastric pH induced by treatment with omeprazole blunts part of the beneficial cardiovascular effects of dietary nitrate and nitrite.
Asunto(s)
Antihipertensivos/administración & dosificación , Jugo Gástrico/química , Nitrito de Sodio/administración & dosificación , Administración Oral , Animales , Antiulcerosos/farmacología , Antihipertensivos/antagonistas & inhibidores , Aorta/efectos de los fármacos , Aorta/fisiopatología , Benzoatos/farmacología , Presión Sanguínea/efectos de los fármacos , Depuradores de Radicales Libres/farmacología , Jugo Gástrico/efectos de los fármacos , Concentración de Iones de Hidrógeno , Hipertensión/inducido químicamente , Hipertensión/tratamiento farmacológico , Imidazoles/farmacología , Técnicas In Vitro , Masculino , NG-Nitroarginina Metil Éster , Óxido Nítrico/sangre , Omeprazol/farmacología , Ratas , Ratas Wistar , S-Nitrosotioles/sangre , Nitrito de Sodio/antagonistas & inhibidores , Vasodilatación/efectos de los fármacosRESUMEN
To provide further evidence for the lipid sink theory, we have developed an in vitro model to assess the effect of Intralipid® 20% on methaemoglobin formation by drugs of varying lipid solubility. Progressively increasing Intralipid concentrations from 4 to 24 mg.ml⻹ suppressed methaemoglobin formation by the lipid soluble drug glyceryl trinitrate in a dose-dependent manner (p < 0.001). Both dose and timing of administration of Intralipid to blood previously incubated with glyceryl trinitrate for 10 and 40 min resulted in significant suppression of methaemoglobin formation (p < 0.0001 and p < 0.05, respectively). Mathematical modelling demonstrated that the entire process of methaemoglobin formation by glyceryl trinitrate was slowed down in the presence of Intralipid. Intralipid did not significantly suppress methaemoglobin formation induced by 2-amino-5-hydroxytoluene (partially lipid soluble) or sodium nitrite (lipid insoluble; both p > 0.5). This work may assist determination of the suitability of drugs taken in overdose for which Intralipid might be deployed.
Asunto(s)
Sangre/efectos de los fármacos , Emulsiones Grasas Intravenosas/farmacología , Lípidos/sangre , Metahemoglobina/antagonistas & inhibidores , Algoritmos , Análisis de los Gases de la Sangre , Relación Dosis-Respuesta a Droga , Humanos , Lípidos/química , Metahemoglobina/biosíntesis , Modelos Estadísticos , Nitroglicerina/antagonistas & inhibidores , Nitroglicerina/química , Nitroglicerina/farmacología , Nitrito de Sodio/antagonistas & inhibidores , Nitrito de Sodio/química , Nitrito de Sodio/farmacología , Solubilidad , Vasodilatadores/antagonistas & inhibidores , Vasodilatadores/química , Vasodilatadores/farmacologíaRESUMEN
In the present study, we investigated the neurobiochemical alterations and oxidative stress induced by food preservative; sodium nitrite (NaNO2) as well as the role of the garlic oil in amelioration of the neurotoxicity in male albino rats. Serum and brain homogenates of the rats received NaNO2 (80 mg/kg body weight) for 3 months exhibited significant decrease in acetylcholine esterase (AChE) activity as well as the levels of phospholipids, total protein and the endogenous antioxidant system (glutathione; GSH and superoxide dismutase; SOD). In contrast, lactic dehydrogenase (LDH) activity, brain thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) levels were significantly increased. On the other hand, the oral administration of garlic oil (5 ml/kg body weight) daily for 3 months significantly improved the neurobiochemical disorders and inhibited the oxidative stress induced by NaNO2 ingestion. So, this study reveals the neural toxic effects of NaNO2 by exerting oxidative stress and retrograde the endogenous antioxidant system. However, garlic oil has a promising role in attenuating the obtained hazard effects of sodium nitrite by its high antioxidant properties which may eventually be related with the preservation of SOD activity and primary mitochondrial role against nitrite-induced neurotoxicity in rats.
Asunto(s)
Compuestos Alílicos/farmacología , Síndromes de Neurotoxicidad/prevención & control , Estrés Oxidativo/efectos de los fármacos , Nitrito de Sodio/antagonistas & inhibidores , Nitrito de Sodio/toxicidad , Sulfuros/farmacología , Acetilcolinesterasa/sangre , Animales , Química Encefálica/efectos de los fármacos , Radicales Libres/metabolismo , L-Lactato Deshidrogenasa/sangre , Peroxidación de Lípido/efectos de los fármacos , Masculino , Óxido Nítrico/metabolismo , Fosfolípidos/sangre , Ratas , Ratas Sprague-Dawley , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
The aim of this study was to elucidate the mechanism by which curing agents, especially nitrite, inhibit the formation of zinc protoporphyrin IX (ZPP) in dry-cured hams such as Parma ham. The oxidation-reduction potential of model solutions was increased by the addition of nitrite, but it was not clear whether the formation of ZPP is inhibited by the oxidizing property of nitrite. The effect of nitric oxide (NO) produced from nitrite on the formation of ZPP was examined. The amount of ZPP formed was decreased by the addition of NO donors. The amount of protoporphyrin IX (PPIX), which is the precursor of ZPP, was also decreased by the addition of NO donors. It is concluded that NO produced from nitrite inhibited the formation of PPIX and ZPP was therefore not formed in cured meat products with the addition of nitrite or nitrate.
Asunto(s)
Conservantes de Alimentos/química , Carne/análisis , Óxido Nítrico/química , Protoporfirinas/química , Nitrito de Sodio/química , Animales , Quelantes/química , Conservación de Alimentos , Modelos Químicos , Donantes de Óxido Nítrico/química , Concentración Osmolar , Oxidación-Reducción , Nitrito de Sodio/antagonistas & inhibidores , Sus scrofaRESUMEN
Sodium nitrite has been shown to have vasodilator activity in experimental animals and in human subjects. However, the mechanism by which nitrite anion is converted to vasoactive nitric oxide (NO) is uncertain. It has been hypothesized that deoxyhemoglobin, xanthine oxidoreductase, mitochondrial aldehyde dehydrogenase, and other heme proteins can reduce nitrite to NO, but studies in the literature have not identified the mechanism in the intact rat, and several studies report no effect of inhibitors of xanthine oxidoreductase. In the present study, the effects of the xanthine oxidoreductase inhibitor allopurinol and the mitochondrial aldehyde dehydrogenase inhibitor cyanamide on decreases in mean systemic arterial pressure in response to i.v. sodium nitrite administration were investigated in the rat. The decreases in mean systemic arterial pressure in response to i.v. administration of sodium nitrite were inhibited in a selective manner after administration of allopurinol in a dose of 25 mg/kg i.v. A second 25 mg/kg i.v. dose had no additional inhibitory effect on the response to sodium nitrite. The decreases in mean systemic arterial pressure in response to sodium nitrite were attenuated by cyanamide and a second 25 mg/kg i.v. dose had no additional inhibitory effect. In L-NAME-treated animals, allopurinol attenuated responses to sodium nitrite and a subsequent administration of cyanamide had no additional effect. When the order of administration of the inhibitors was reversed, responses to sodium nitrite were attenuated by administration of cyanamide and a subsequent administration of allopurinol had no additional inhibitory effect. The results of these studies suggest that nitrite can be reduced to vasoactive NO in the systemic vascular bed of the rat by xanthine oxidoreductase and mitochondrial aldehyde dehydrogenase and that the 2 pathways of nitrite activation act in a parallel manner.
Asunto(s)
Aldehído Deshidrogenasa/fisiología , Presión Sanguínea/efectos de los fármacos , Nitrito de Sodio/farmacología , Xantina Deshidrogenasa/fisiología , 1-(5-Isoquinolinesulfonil)-2-Metilpiperazina/análogos & derivados , 1-(5-Isoquinolinesulfonil)-2-Metilpiperazina/farmacología , Aldehído Deshidrogenasa/antagonistas & inhibidores , Aldehído Deshidrogenasa/metabolismo , Alopurinol/farmacología , Animales , Presión Sanguínea/fisiología , Cianamida/farmacología , Relación Dosis-Respuesta a Droga , Masculino , Mitocondrias/efectos de los fármacos , Mitocondrias/fisiología , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Nitroprusiato/farmacología , Ratas , Ratas Sprague-Dawley , Nitrito de Sodio/antagonistas & inhibidores , Vasodilatadores/farmacología , Xantina Deshidrogenasa/antagonistas & inhibidores , Xantina Deshidrogenasa/metabolismoRESUMEN
The aim of the present study was to examine the chemopreventive efficacy of S-methylcysteine (SMC) on rat hepatocarcinogenesis induced by concurrent administration of sodium nitrite (NaNO(2)) and morpholine (Mor) using a medium-term rat liver carcinogenesis bioassay (Ito test). Administration of SMC caused significant reduction in the areas of glutathione S-transferase placental form positive foci along with a significant decrease of hepatocyte 5-bromo-2'-deoxyuridine (BrdU) labeling indices. These results demonstrated potent chemopreventive effects of SMC against hepatocarcinogenesis due to concurrent administration of Mor and NaNO(2). SMC could thus be an effective chemopreventive agent for decreasing the risk of carcinogenicity from environmental precursors of N-nitroso compounds.
Asunto(s)
Anticarcinógenos/farmacología , Cisteína/farmacología , Conservantes de Alimentos/toxicidad , Neoplasias Hepáticas Experimentales/prevención & control , Morfolinas/toxicidad , Nitrito de Sodio/toxicidad , Animales , Peso Corporal/efectos de los fármacos , Bromodesoxiuridina/metabolismo , Carcinógenos/farmacocinética , Carcinógenos/toxicidad , División Celular/efectos de los fármacos , Cisteína/análogos & derivados , Interacciones Farmacológicas , Conservantes de Alimentos/farmacocinética , Mucosa Gástrica/metabolismo , Glutatión Transferasa/metabolismo , Hígado/anatomía & histología , Hígado/efectos de los fármacos , Neoplasias Hepáticas Experimentales/inducido químicamente , Neoplasias Hepáticas Experimentales/enzimología , Masculino , Morfolinas/antagonistas & inhibidores , Morfolinas/farmacocinética , Nitrosaminas/metabolismo , Lesiones Precancerosas/inducido químicamente , Lesiones Precancerosas/enzimología , Lesiones Precancerosas/prevención & control , Ratas , Ratas Endogámicas F344 , Nitrito de Sodio/antagonistas & inhibidores , Nitrito de Sodio/farmacocinéticaRESUMEN
The inhibiting effect of organic Se (selen-enriched yeast Bioselen) on the endogenous synthesis of N-nitrosubstances was investigated in the Wistar rats, receiving 15 mg of sodium nitritis and 24 mg of diethylamin per 1 kg of bodyweight during 22 days. The level of nitrosoprolin synthesis and (NPro) and the level of nitrosodiethyl (NDie) in the stomach of rats served as the main indices. The highest level of NPro and NDie were revealed in the rats, without selen supplementation (581.2 +/- 113.3 mg per kg of bodyweight and 29.8 +/- 3.0 mg per kg of bodyweight). The highest inhibiting effect of Se was 54.5% for NPro and 54.7% for NDie and it was shown for the Se concentration of 1.5 mg per 1 kg of forage. The increase of Se dosage to 3.0 mg per 1 kg of forage was less effective and resulted in 25.5% of inhibiting of NPro u 47.0% - NDie.
Asunto(s)
Dietilnitrosamina/metabolismo , Mutágenos/metabolismo , Nitrosaminas/metabolismo , Selenio/farmacología , Administración Oral , Animales , Suplementos Dietéticos , Dietilaminas/antagonistas & inhibidores , Dietilnitrosamina/análisis , Relación Dosis-Respuesta a Droga , Mucosa Gástrica/metabolismo , Nitrosaminas/análisis , Ratas , Ratas Wistar , Selenio/administración & dosificación , Nitrito de Sodio/antagonistas & inhibidores , Estómago/efectos de los fármacosRESUMEN
The effect of ascorbic, ferulic and caffeic acids on dimethylamine and amidopyrine nitrosation in a medium containing 3 samples of total gastric juice taken from 10 humans (pH = 6.1; 3.2 and 1.7) was studied versus the amount of inhibitor added to the medium. The resultant relationship proved bizarre, the inhibitor at low concentrations stimulating nitrosation in some situations. When gastric juice concentration in the medium was further lowered by dilution with a buffer at relevant value of pH, the paradoxical effect of inhibition gradually disappeared.
Asunto(s)
Jugo Gástrico/efectos de los fármacos , Nitrosaminas/antagonistas & inhibidores , Aminopirina/análisis , Aminopirina/antagonistas & inhibidores , Aminopirina/farmacocinética , Animales , Ácido Ascórbico/farmacología , Ácidos Cafeicos/farmacología , Cromatografía de Gases , Ácidos Cumáricos/farmacología , Dimetilaminas/análisis , Dimetilaminas/antagonistas & inhibidores , Dimetilaminas/farmacocinética , Jugo Gástrico/análisis , Jugo Gástrico/metabolismo , Humanos , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Ratones , Ratones Endogámicos , Nitrosaminas/análisis , Nitrosaminas/farmacocinética , Nitrito de Sodio/análisis , Nitrito de Sodio/antagonistas & inhibidores , Nitrito de Sodio/farmacocinéticaRESUMEN
The addition of sodium nitrite to washed bovine erythrocytes incubated in buffered saline resulted in the formation of methemoglobin with a decrease in the concentration of 3-N-ribosyluric acid. The oxyhemoglobin in hemolysates prepared from bovine red cells which contained high concentrations of 3-ribosyluric acid was oxidized to methemoglobin more slowly than oxyhemoglobin from cells with low levels of 3-ribosyluric acid. Oxyhemoglobin from hemolysates that were dialyzed was oxidized more rapidly than oxyhemoglobin which was not dialyzed. 3-Ribosyluric acid, glutathione, uric acid, and ascorbic acid prevented the oxidation of oxyhemoglobin by nitrite. Uric acid protected oxyhemoglobin at the lowest concentration, followed closely by ascorbic acid, 3-ribosyluric acid, and glutathione. Hydrogen peroxide enhanced the oxidation produced by nitrite; this effect was also prevented by the four antioxidants used.
Asunto(s)
Nitritos/antagonistas & inhibidores , Oxihemoglobinas/metabolismo , Ribonucleósidos/farmacología , Nitrito de Sodio/antagonistas & inhibidores , Ácido Úrico/análogos & derivados , Animales , Ácido Ascórbico/farmacología , Bovinos , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Glutatión/farmacología , Hemólisis , Peróxido de Hidrógeno/farmacología , Técnicas In Vitro , Metahemoglobina/biosíntesis , Oxidación-Reducción/efectos de los fármacos , Nitrito de Sodio/farmacología , Ácido Úrico/farmacologíaRESUMEN
Relaxation by nitroglycerin, sodium nitrite, and amyl nitrite of bovine coronary arterial smooth muscle was inhibited by the oxidant methylene blue. Methylene blue also inhibited activation of bovine coronary arterial soluble guanylate cyclase by nitroglycerin, which required addition of cysteine. At concentrations less than 10 mM, sodium nitrite required the addition of one of several thiols or ascorbate to activate guanylate cyclase from bovine coronary artery. Guanylate cyclase activation by large amounts (50 microL) of saturated amyl nitrite gas did not require, but was enhanced by, the addition of thiols or ascorbate. However, similar to sodium nitrite, guanylate cyclase activation by smaller amounts (5 microL) of saturated amyl nitrite gas did require the addition of one of various thiols or ascorbate. Methylene blue markedly inhibited guanylate cyclase activation by sodium nitrite in the presence of cysteine or ascorbate and similarly inhibited enzyme activation by amyl nitrite either in the absence or presence of cysteine or ascorbate. These data support the hypothesis that nitrates and nitrites relax vascular smooth muscle by stimulating cyclic GMP formation. The results further suggest that, similar to relaxation and guanylate cyclase activation by nitroso-containing compounds, relaxation and enzyme activation by nitrates and and nitrites may involve the formation of nitric oxide or complexes of nitric oxide as active intermediates.
Asunto(s)
Vasos Coronarios/efectos de los fármacos , Guanilato Ciclasa/metabolismo , Azul de Metileno/farmacología , Contracción Muscular/efectos de los fármacos , Relajación Muscular/efectos de los fármacos , Nitrito de Amila/antagonistas & inhibidores , Animales , Bovinos , Activación Enzimática/efectos de los fármacos , Técnicas In Vitro , Músculo Liso Vascular/efectos de los fármacos , Nitroglicerina/antagonistas & inhibidores , Nitrito de Sodio/antagonistas & inhibidoresRESUMEN
Bis(2,2-dimethyl-4-methane sulphonic acid sodium salt-1,2-dihydroquinoline)-6,6'-methane (MTDQ-DA), a new, non-toxic, water soluble antioxidant, is shown to inhibit liver necrosis induced in rats by N-nitrosomorpholine (N-MOR), itself formed in vivo following the administration simultaneously of morphine (MOR) and sodium nitrite (NaNO2).
Asunto(s)
Antioxidantes/farmacología , Hígado/efectos de los fármacos , Morfolinas/antagonistas & inhibidores , Nitritos/antagonistas & inhibidores , Quinolinas/farmacología , Nitrito de Sodio/antagonistas & inhibidores , Animales , Hígado/patología , Masculino , Morfolinas/metabolismo , Necrosis , Nitrosaminas/metabolismo , Ratas , Nitrito de Sodio/metabolismoRESUMEN
The addition of sodium metabisulfite as a source of sulfur dioxide delayed botulinal outgrowth in perishable canned comminuted pork when it was temperature abused at 27 degree C. The degree of inhibition was directly related to the level of sulfur dioxide. Levels greater than 100 microgram of sulfur dioxide per g were necessary to achieve significant inhibition when a target level of 100 botulinal spores per g was used. Sodium nitrite partially reduced the efficacy of the sulfur dioxide. Sulfur dioxide offers a new option for the control of botulinal outgrowth in cured or noncured meat and poultry products.