RESUMEN
The N95 filtering facepiece respirator (FFR) is commonly used to protect individuals from infectious aerosols. Health care experts predict a shortage of N95 FFRs if a severe pandemic occurs, and an option that has been suggested for mitigating such an FFR shortage is to decontaminate and reuse the devices. Before the effectiveness of this strategy can be established, many parameters affecting respiratory protection must be measured: biocidal efficacy of the decontamination treatment, filtration performance, pressure drop, fit, and toxicity to the end user post treatment. This research effort measured the amount of residual chemicals created or deposited on six models of FFRs following treatment by each of 7 simple decontamination technologies. Measured amounts of decontaminants retained by the FFRs treated with chemical disinfectants were small enough that exposure to wearers will be below the permissible exposure limit (PEL). Toxic by-products were also evaluated, and two suspected toxins were detected after ethylene oxide treatment of FFR rubber straps. The results provide encouragement to efforts promoting the evolution of effective strategies for decontamination and reuse of FFRs.
Asunto(s)
Descontaminación/métodos , Oxidantes/análisis , Dispositivos de Protección Respiratoria , Compuestos Epoxi/análisis , Humanos , Peróxido de Hidrógeno/análisis , Ensayo de Materiales , Filtros Microporos , Hipoclorito de Sodio/análisisRESUMEN
BACKGROUND: Recent data have demonstrated increased lipid peroxidation (LPO) levels and oxidative stress in periodontitis. Malondialdehyde (MDA) and superoxide dismutase (SOD) are both increased during oxidative stress. Furthermore, this study examined SOD concentration, total oxidative status (TOS) and MDA levels in periodontal patients and investigated the longitudinal effect of periodontal therapy on the index levels of chronic periodontitis (CP) patients. METHODS: Serum, saliva and gingival crevicular fluid (GCF) samples were obtained from 48 CP patients and 35 healthy control subjects prior to, as well as after 16 weeks following non-surgical post-periodontal therapy. MDA, TOS and SOD and clinical parameters were determined pre- and post-therapy. RESULTS: The levels of TOS and SOD values were significantly higher in the CP group than in the control group (p < 0.05), but only MDA in GCF. Post-periodontal therapy, serum, saliva and GCF TOS and SOD levels significantly decreased compared to basal levels (p < 0.05), but only MDA in GCF. CONCLUSIONS: LPO was higher in the periodontal region, with TOS and SOD increasing both locally and peripherally. Non-surgical therapy can restore and control the subject antioxidant capacity by locally and systemically modifying the levels of MDA, TOS and SOD.
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Periodontitis Crónica/terapia , Depuradores de Radicales Libres/sangre , Líquido del Surco Gingival/química , Peroxidación de Lípido/fisiología , Oxidantes/sangre , Saliva/química , Superóxido Dismutasa/sangre , Adulto , Pérdida de Hueso Alveolar/diagnóstico por imagen , Periodontitis Crónica/sangre , Periodontitis Crónica/metabolismo , Índice de Placa Dental , Raspado Dental , Femenino , Estudios de Seguimiento , Depuradores de Radicales Libres/análisis , Líquido del Surco Gingival/enzimología , Hemorragia Gingival/terapia , Humanos , Estudios Longitudinales , Masculino , Malondialdehído/análisis , Malondialdehído/sangre , Higiene Bucal , Oxidantes/análisis , Estrés Oxidativo/fisiología , Pérdida de la Inserción Periodontal/terapia , Índice Periodontal , Bolsa Periodontal/terapia , Periodoncio/enzimología , Periodoncio/metabolismo , Radiografía , Aplanamiento de la Raíz , Saliva/enzimología , Curetaje Subgingival , Superóxido Dismutasa/análisisRESUMEN
A randomized complete block design with three replications was utilized to evaluate the effects (P<0.05) of various fumigation concentrations of sulfuryl fluoride (SF) (0, 12, 24, 36 and 72 mg/L) on the fluoride, sulfuryl fluoride, and volatile flavor compound concentrations in dry cured ham. Fluoride and SF concentrations increased (P<0.05) in dry cured hams as SF fumigation concentration increased, but all samples contained fluoride and SF concentrations below the legal limits of 20 and 0.01 ppm, respectively. Minimal differences existed (P>0.05) in the presence and concentration of aroma active compounds, but oxidation compounds were slightly more prevalent (P<0.05) in the 72 mg/ml treatment when compared to the control. A triangle test (n=54) indicated that consumers could not discern (P>0.75) between hams that were fumigated with SF at 0 and 36 mg/ml. This study revealed that there were minimal aroma/flavor differences among the ham treatments between 0 and 36 mg/ml, and that dry cured ham that was fumigated with SF was safe and met legal requirements for consumption.
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Seguridad de Productos para el Consumidor , Conservación de Alimentos/métodos , Carne/análisis , Odorantes , Ácidos Sulfínicos , Gusto , Compuestos Orgánicos Volátiles/análisis , Animales , Seguridad de Productos para el Consumidor/legislación & jurisprudencia , Fluoruros/análisis , Fumigación , Humanos , Legislación Alimentaria , Carne/normas , Oxidantes/análisis , Oxidación-Reducción , Distribución Aleatoria , Valores de Referencia , Sensación , Ácidos Sulfínicos/análisis , PorcinosRESUMEN
Many polyphenolic compounds are poorly digested, and have low bioavailability due to their long chain lengths and chemical composition. A processed, flavanol-rich lychee fruit extract (FRLFE) that is higher in flavanol monomers, dimer and trimers than its unprocessed counterpart, was tested in a variety of models. First, mature visceral adipocytes were treated with 0, 3, 10 or 30 microg/mL FRLFE (day 6-8). Compared with the controls, the treated cells had lower triglyceride concentrations, less lipid accumulation and a smaller lipid droplet size. Adiponectin release was significantly greater in cells receiving 3 or 10 microg/mL FRLFE than in the controls. Second, rats given a single dose of 50 or 100 mg/kg FRLFE had significant increases in plasma (-)-epicatechin, 3'-O-methyl-(-)-epicatechin, and (+)-catechin levels, peak values were at approximately 2 h and appreciable concentrations were still detected at 6 h. Rats supplemented daily for 1 week with 50 or 100 mg/kg FRLFE had significantly elevated metabolite concentrations. In response to an oxidative stress, erythrocyte membrane integrity was significantly improved in the 100 mg/kg FRLFE group. Third, 7-month-old mice fed a 200 mg/kg FRLFE diet for 10 months showed a significant decrease in glucose, triglyceride and lipid peroxide levels compared with mice fed a control diet. Collectively, these results support the concept that the flavanols present in FRLFE are well absorbed and bioactive.
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Adipocitos/efectos de los fármacos , Flavonoles/farmacología , Litchi/química , Síndrome Metabólico/tratamiento farmacológico , Extractos Vegetales/farmacología , Adiponectina/biosíntesis , Animales , Glucemia , Catequina/sangre , Células Cultivadas , Eritrocitos/efectos de los fármacos , Frutas/química , Masculino , Ratones , Modelos Animales , Oxidantes/análisis , Estrés Oxidativo , Ratas , Ratas Sprague-Dawley , Triglicéridos/análisisRESUMEN
BACKGROUND: In previous studies, it has been demonstrated that Nigella Sativa (NS) has protective effects against ischemia reperfusion injury on various organs. However, its protective effects on intestinal tissue against ischemia reperfusion injury are unclear. We aimed to determine whether NS prevents intestinal ischemia-reperfusion injury in rats. MATERIALS AND METHODS: Thirty rats were divided into three groups as sham (group 1), control (group 2), and NS-treatment group (group 3). All rats underwent intestinal ischemia for 60 min followed by a 60-min period of reperfusion. Rats were intraperitoneally infused only 0.9% saline solutions in group 2. Rats in the group 3 received NS (0,2 mL/kg) intraperitoneally, before ischemia and before reperfusion. Total antioxidant capacity (TAC), catalase (CAT), total oxidative status (TOS), oxidative stress index (OSI), and myeloperoxidase (MPO) in ileum tissue were measured. Also, ileum tissue histopathology was evaluated by a light microscope. RESULTS: The levels of liver enzymes in group 3 were significantly lower than those in group 2 (p <.01). TAC and CAT activity levels in ileum tissue were significantly higher in group 3 than in group 2. TOS, OSI, and MPO in ileum tissue were significantly lower in group 3 than group 2 (p <.05 for TOS and MPO; p < .01 for OSI). Histological tissue damage was milder in the NS treatment group than in the control group. CONCLUSION: Our results suggest that NS treatment protected the rat's intestinal tissue against intestinal ischemia-reperfusion injury.
Asunto(s)
Íleon/irrigación sanguínea , Nigella sativa , Aceites de Plantas/uso terapéutico , Daño por Reperfusión/prevención & control , Animales , Antioxidantes/análisis , Catalasa/análisis , Constricción , Evaluación Preclínica de Medicamentos , Íleon/química , Íleon/patología , Inyecciones Intraperitoneales , Masculino , Arteria Mesentérica Superior , Oxidantes/análisis , Estrés Oxidativo/efectos de los fármacos , Peroxidasa/análisis , Fitoterapia , Aceites de Plantas/administración & dosificación , Ratas , Ratas WistarRESUMEN
The aim of this study was to investigate total antioxidant (TAC), and oxidant capacity (TOC) and nitric oxide (NO) levels in milk of cows with subclinical mastitis. Brown Swiss and Holstein breed cows were screened with California Mastitis Test (CMT) to determine mammary glands with subclinical mastitis. Moreover, somatic cell counts (SCC) were determined electronically in all milk samples. Mammary quarters were classified as healthy (n=25) or subclinical mastitis (n=35) based on CMT scores and somatic cell count (SCC: < or =200,000/ml or >200,000/ml) in milk. Nitric oxide, TOC and SCC levels were significantly higher (p<0.001, p<0.005 and p<0.001, respectively) in milk from mammary quarters with subclinical mastitis compared to those from healthy mammary quarters. In conclusion, subclinical mastitis results in higher NO concentrations, TOC and SCC, and NO and TOC were positively correlated with SCC. Moreover, alterations in NO levels and TOC in milk could be used as an alternative diagnostic tool to screen for subclinical mastitis.
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Antioxidantes/metabolismo , Mastitis Bovina/metabolismo , Leche/química , Óxido Nítrico/metabolismo , Oxidantes/metabolismo , Animales , Antioxidantes/análisis , Bovinos , Recuento de Células/veterinaria , Femenino , Óxido Nítrico/análisis , Oxidantes/análisisRESUMEN
Sunflower oil at a specific oxidation stage (when several oxygenated alpha,beta-unsaturated aldehydes are generated, mainly 4-hydroperoxy-trans-2-alkenals and 4-hydroxy-trans-2-alkenals), caused at 70 degrees C with aeration for 7 days, was administered intraperitoneally to rats. This oil was studied by means of solid phase micro-extraction followed by gas chromatography/mass spectrometry (SPME-GC/MS) and by proton nuclear magnetic resonance ((1)H-NMR). Oxidized sunflower oil (3 ml/kg/day) was administered to male Sprague-Dawley rats for 21 days. The control group was administered non-oxidized sunflower oil in the same volume and for the same duration as the experimental group. A significant decrease in the number of neural cells positively immunostained for TrkA receptor was detected in the frontal cortex of the experimental group, with respect to controls, suggesting both neuronal damage as well as a deficit in neuronal survival signalling at this level. This could lead to apoptosis of cholinergic neurons, which play a key role in memory and attention function. These results indicate that toxic substances present in the oxidized sunflower oil, among them 4-hydroxy-trans-2-nonenal (HNE) and 4-hydroperoxy-trans-2-nonenal (HPNE), could disrupt survival signalling of frontal cortex cholinergic neurons, which could lead to apoptosis and neurodegenerative diseases. In the case of humans, this fact reinforces the necessity of avoiding the re-utilization of oxidized sunflower oil, in order to contribute to long-term neurodegenerative diseases prevention.
Asunto(s)
Aldehídos/toxicidad , Lóbulo Frontal/patología , Peróxidos Lipídicos/toxicidad , Neuronas/metabolismo , Oxidantes/toxicidad , Aceites de Plantas/toxicidad , Receptor trkA/metabolismo , Aldehídos/análisis , Animales , Cromatografía de Gases y Espectrometría de Masas , Calor , Inmunohistoquímica , Inyecciones Intraperitoneales , Peróxidos Lipídicos/análisis , Espectroscopía de Resonancia Magnética , Masculino , Enfermedades Neurodegenerativas/etiología , Enfermedades Neurodegenerativas/prevención & control , Oxidantes/análisis , Oxidación-Reducción , Aceites de Plantas/administración & dosificación , Aceites de Plantas/química , Ratas , Ratas Sprague-Dawley , Microextracción en Fase Sólida , Aceite de Girasol , Factores de TiempoRESUMEN
Fluorescamine derivatized 3-amino-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (I) is shown to undergo an irreversible reaction with peroxyl radicals and other radical oxidants to generate a more highly fluorescent diamagnetic product (II) and thus can be used as a highly sensitive and versatile probe to determine oxidant production optically, either by monitoring the changes in fluorescence intensity, by HPLC analysis with fluorescence detection, or by a combination of both approaches. By changing the [O2]/[I] ratio, we show that peroxyl radicals can be detected and quantified preferentially in the presence of other radical oxidants. Detection of photochemically produced peroxyl radicals is achieved by employing 3-amino-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (3-ap) alone, followed by derivatization with fluorescamine. With employment of HPLC analysis, the detection limit of II at a S/N of 2 is approximately 3 nM for a 125 microL injection. Preliminary applications include the detection of peroxyl radicals generated thermally in soybean phosphatidylcholine liposomes and produced photochemically in tap water.
Asunto(s)
Óxidos N-Cíclicos/química , Fluorescamina/química , Indicadores y Reactivos/química , Oxidantes/análisis , Peróxidos/análisis , Cromatografía Líquida de Alta Presión , Liposomas/química , Oxidantes/química , Peróxidos/químicaRESUMEN
Oligonucleotide that can reversibly alter the duplex-forming ability in response to an external factor has potential to be applied to various genome technologies, e.g. antisense and gene diagnosis technology. A novel bridged nucleoside which is capable of sensing an oxidant and reductant was designed and synthesized. The nucleoside formed a disulfide bridge between C2' and C4' positions, which would enhance its duplex-forming ability under oxidative conditions. Contrastingly, the disulfide bridge was cleaved under reductive conditions; it could cause decrease of duplex-forming ability.
Asunto(s)
Nucleósidos/química , Timidina/análogos & derivados , Nucleósidos/síntesis química , Oxidantes/análisis , Oxidación-Reducción , Sustancias Reductoras/análisis , Timidina/síntesis química , Timidina/químicaRESUMEN
It has been suggested that the very low incidence of atherosclerosis in glycogen storage disease type Ia (GSD Ia) subjects might be attributed to elevated levels of uric acid, one of the potent low molecular- weight antioxidants found in plasma. The present communication describes a use of two analytical methods-cyclic voltammetry and ferric reducing ability of plasma-and also two chemiluminescence methods to evaluate the total oxidant-scavenging capacities (TOSC) of plasma from GSD Ia patients. Our results verified the elevation of TOSC in GSD Ia patients and we propose the inclusion of luminescence and cyclic voltammetry assays as reliable methods for estimating TOSC in a variety of clinical disorders. Our findings with the cyclic voltammetry method add support to the assumption that the elevated uric acid levels might be the main contributor to plasma antioxidant capacity and possible protection against atherosclerosis.
Asunto(s)
Recuperación de Fluorescencia tras Fotoblanqueo , Depuradores de Radicales Libres/sangre , Enfermedad del Almacenamiento de Glucógeno Tipo I/sangre , Mediciones Luminiscentes/métodos , Oxidantes/sangre , Adolescente , Adulto , Estudios de Casos y Controles , Niño , Preescolar , Conductividad Eléctrica , Electroquímica/métodos , Depuradores de Radicales Libres/análisis , Enfermedad del Almacenamiento de Glucógeno Tipo I/diagnóstico , Humanos , Luminol/química , Oxidantes/análisis , Oxidación-Reducción , Reproducibilidad de los Resultados , Adulto JovenRESUMEN
BACKGROUND: Polymorphonuclear leukocytes (PMNs) from subjects with localized aggressive periodontitis (LAgP) present multiple functional abnormalities associated with a phenotypically primed PMN phenotype. Local inflammation is characterized by hypoxia, which leads to increased production of superoxide (O(2)(-)) by PMNs. Ceruloplasmin (CP) is also induced by hypoxia and inflammation. The aim of this study was to investigate the role of CP in O(2)(-) generation in PMNs from healthy subjects and patients with LAgP. METHODS: PMNs were isolated from healthy subjects and those with LAgP (N = 36). Superoxide was measured by cytochrome-C reduction at 550 nm. Intracellular CP expression was analyzed by real-time polymerase chain reaction and Western blotting. Serum levels of CP were measured by enzyme-linked immunosorbent assay. Intracellular iron ion conversion was spectrophotometrically determined by measuring the absorbance of sigma-phenanthroline at 510 nm. RESULTS: O(2)(-) generation was significantly higher in LAgP PMNs before and after stimulation with formyl-methionyl-leucyl-phenylalanine (100 nM). CP expression in PMNs and CP levels in serum were significantly higher in subjects with LAgP compared to the PMNs and serum samples from matched healthy donors (P <0.05). LAgP PMNs also had significantly higher levels of Fe(3+) and lower levels of Fe(2+) compared to healthy PMNs (P <0.05), suggesting increased iron conversion. Exogenous CP treatment of healthy PMNs resulted in significant increases in O(2)(-) generation and iron ion conversion similar to LAgP PMNs. CONCLUSION: LAgP PMNs are primed to express higher levels of CP, leading to hypoxia-mediated O(2)(-) generation in PMNs and increased oxidative stress and neutrophil-mediated tissue injury in LAgP.
Asunto(s)
Periodontitis Agresiva/inmunología , Ceruloplasmina/análisis , Neutrófilos/enzimología , Adolescente , Adulto , Western Blotting , Estudios de Casos y Controles , Ceruloplasmina/efectos de los fármacos , Ceruloplasmina/metabolismo , Citocromos c/metabolismo , Femenino , Compuestos Férricos/análisis , Compuestos Ferrosos/análisis , Humanos , Hipoxia/enzimología , Hipoxia/metabolismo , Masculino , N-Formilmetionina Leucil-Fenilalanina/farmacología , Activación Neutrófila/efectos de los fármacos , Activación Neutrófila/inmunología , Neutrófilos/efectos de los fármacos , Oxidantes/análisis , Oxidantes/metabolismo , Oxidación-Reducción , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Espectrofotometría , Superóxidos/análisis , Superóxidos/metabolismo , Adulto JovenRESUMEN
Oxidative stress plays a pivotal role in the ageing process and in the pathogenesis of numerable diseases. The quantification of the phenomenon is of paramount importance. In the present study, we introduce a novel and simple assay, the Blue CrO(5) assay, for the evaluation of the oxidant and antioxidant capacity of various biological samples and known antioxidants. Chromium peroxide (CrO(5)) is produced by ammonium dichromate in an acidic environment in the presence of H(2)O(2). It is a deep blue potent oxidant compound, miscible and relatively stable in polar organic solvents, that can be easily measured by spectrophotometry. Its reduction by known antioxidants, both water- and lipid-soluble (ascorbate and alpha-tocopherol, respectively, in this study), detected spectrophotometrically as a decrease in the absorption and depicted in EPR spectra, can act as a measure of the antioxidant capacity of a certain compound. The assay displays significant sensitivity, stability, linearity, specificity and repeatability.
Asunto(s)
Antioxidantes/análisis , Compuestos de Cromo/química , Oxidantes/análisis , Peróxidos/química , Espectrofotometría/métodos , Ácido Ascórbico/análisis , Humanos , Oxidación-Reducción , Plasma/metabolismo , Sensibilidad y Especificidad , alfa-Tocoferol/análisisRESUMEN
Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of the parent amino acid residue, formation of unstable intermediates, and the generation of stable products. Each of these events can be used, to a greater or lesser extent, to quantify damage to proteins. In this review the advantages and disadvantages of a number of these approaches are discussed, with an emphasis on methods that yield absolute quantitative data on the extent of protein modification. Detailed methods sheets are provided for many of these techniques.
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Bioquímica/métodos , Biomarcadores/análisis , Oxidantes/análisis , Procesamiento Proteico-Postraduccional , Proteínas/metabolismo , Animales , Cromatografía Líquida de Alta Presión , Ensayo de Inmunoadsorción Enzimática , Humanos , Espectrometría de Masas , Oxidación-Reducción , Estrés Oxidativo , Proteínas/química , Proteínas/inmunología , Especies Reactivas de Oxígeno , Detección de Spin , Estadística como AsuntoRESUMEN
BACKGROUND/OBJECTIVES: To measure reactive oxidant production and the decline in antioxidant potential in commercially available, irradiated parenteral nutrition (PN) solutions and the effect that these have on oxidant production in patients in the intensive care unit. SUBJECTS AND METHODS: Vitamin E and malondialdehyde in irradiated and nonirradiated commercially available, PN solutions were measured. The PBN (alpha-phenyl-n-test-butylnitrone (PBN) spin trap was used to measure free radicals and TEMPOL (2,2,6,6-tetramethyl-4-hydroxy-piperidine-oxyl) was used to assess antioxidant capacity. The irradiated PN was administered (as per unit protocol) to 10 patients with gut failure and plasma and urinary isoprostanes and interleukin-6 (IL-6) were measured 1 hour preadministration, at the time of, and 1 and 2 hours postadministration of PN. RESULTS: Irradiation reduced vitamin E significantly (P < .0025). Malondialdehyde products were present in both samples, but more so in irradiated samples (P < .0001), as were free radicals measured by PBN spin trapping. Irradiated samples had a higher scavenging capacity of TEMPOL free radical due to depletion of antioxidants in irradiated samples. Urinary isoprostanes increased at time 2 by 6.3 units relative to time 0 and by 5.23 units relative to time 1(Friedman ANOVA: P < .01413). CONCLUSIONS: Lipid hydroperoxides are formed in PN solutions and increase further following irradiation. This is associated with a significant reduction in vitamin E and antioxidant potential. The increase in urinary isoprostanes indicates a potentially proinflammatory effect of irradiated PN.
Asunto(s)
Antioxidantes/análisis , Inflamación/etiología , Oxidantes/análisis , Nutrición Parenteral , Adulto , Óxidos N-Cíclicos/análisis , Radicales Libres/análisis , Humanos , Unidades de Cuidados Intensivos , Isoprostanos/orina , Peróxidos Lipídicos/análisis , Malondialdehído/análisis , Persona de Mediana Edad , Soluciones/química , Soluciones/efectos de la radiación , Marcadores de Spin , Vitamina E/análisisRESUMEN
The degradation of organic substances present in winery wastewater was studied in a pilot-scale, bubble column ozonation reactor. A steady reduction of chemical oxygen demand (COD) was observed under the action of ozone at the natural pH of the wastewater (pH 4). At alkaline and neutral pH the degradation rate was accelerated by the formation of radical species from the decomposition of ozone. Furthermore, the reaction of hydrogen peroxide (formed from natural organic matter in the wastewater) and ozone enhances the oxidation capacity of the ozonation process. The monitoring of pH, redox potential (ORP), UV absorbance (254 nm), polyphenol content and ozone consumption was correlated with the oxidation of the organic species in the water. The ozonation of winery wastewater in the bubble column was analysed in terms of a mole balance coupled with ozonation kinetics modeled by the two-film theory of mass transfer and chemical reaction. It was determined that the ozonation reaction can develop both in and across different kinetic regimes: fast, moderate and slow, depending on the experimental conditions. The dynamic change of the rate coefficient estimated by the model was correlated with changes in the water composition and oxidant species.
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Residuos Industriales , Ozono , Eliminación de Residuos Líquidos/métodos , Vino , Biodegradación Ambiental , Flavonoides/análisis , Peróxido de Hidrógeno , Concentración de Iones de Hidrógeno , Cinética , Oxidantes/análisis , Oxidación-Reducción , Fenoles/análisis , Proyectos Piloto , Polifenoles , PortugalRESUMEN
Here, we investigated the effect of induction of the Epstein-Barr virus (EBV) viral lytic cycle on the oxidant/antioxidant balance in three lymphoblastoid cell lines: B95-8, Raji, and LCL C1. The induction of the EBV lytic cycle was done by a non-stressing dose of 12-0-tetradecanoylphorbol-13-acetate (8 nM). Oxidative stress was assessed by measuring malondialdehyde as a parameter of lipid peroxidation, the levels of glutathione, and the activities of three antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase). After 48 h (peak of lytic cycle), a significant decrease in superoxide dismutase activity was observed in B95-8, Raji, and LCL C1 cells (P < 0.05). In addition, in B95-8 cells also a significant decrease of catalase activity was detected (P < 0.05). The glutathione peroxidase activity and the glutathione level were not significantly modified by the induction in any of the cell lines. We found a significant rise in malondialdehyde levels in B95-8, Raji, and LCL C1 cells after the induction of the lytic cycle compared to controls (P < 0.05). In conclusion, induction of EBV lytic cycle in lymphoblastoid cells causes increased oxidative stress in the host cells within 48 h, a process that could be involved in malignant transformations.
Asunto(s)
Transformación Celular Viral , Infecciones por Virus de Epstein-Barr/metabolismo , Herpesvirus Humano 4/fisiología , Leucemia de Células B/virología , Estrés Oxidativo , Antioxidantes/análisis , Biomarcadores/análisis , Línea Celular Tumoral , Humanos , Leucemia de Células B/metabolismo , Leucemia de Células B/patología , Oxidantes/análisis , Oxidorreductasas/metabolismoRESUMEN
Fe-C-TiO(2) photocatalysts were prepared by mechanical mixing of commercial anatase TiO(2) precursor with FeC(2)O(4) and heating at 500-800 degrees C under argon flow. These photocatalysts were tested for dyes decomposition: Methylene Blue (MB), Reactive Black (RB) and Acid Red (AR). The preliminary adsorption of dyes on the photocatalysts surface was performed. Modification of anatase by FeC(2)O(4) caused reducing of zeta potential of the photocatalyst surface from +12 to -7mV and decreasing of their adsorption ability towards RB and AR, which were negatively charged, -46.8 and -39.7, respectively. Therefore, unmodified TiO(2) showed the highest degree of RB and AR decompositions in the combination of dyes adsorption and UV irradiation. Methylene Blue, which had zeta potential of +4.3 in the aqueous solution was poorly adsorbed on all the tested photocatalysts and also slowly decomposed under UV irradiation. The high rate of dyes decomposition was noted on Fe-C-TiO(2) photocatalysts under UV irradiation with addition of H(2)O(2). It was observed, that at lower temperatures of heat treatment such as 500 degrees C higher content of carbon is remained in the sample, blocking the built in of iron into the TiO(2) lattice. This iron is reactive in the photo-Fenton process resulting in high production of OH radicals and also high activity of the photocatalyst. At higher temperatures of heat treatment, less active FeTiO(3) phase is formed, therefore Fe-C-TiO(2) sample prepared at 800 degrees C showed low photocatalytic activity for dyes decomposition. Fe-C-TiO(2) photocatalysts are active under visible light irradiation, however, the efficiency of a dye decomposition is lower than under UV light. In a dark Fenton process there is observed an insignificant generation of OH radicals and very little decomposition of a dye, what suggests the powerful of photo-Fenton process in the dyes decomposition.
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Colorantes/química , Colorantes/efectos de la radiación , Rayos Ultravioleta , Carbono/análisis , Catálisis , Electroquímica , Radical Hidroxilo/análisis , Indicadores y Reactivos , Oxalatos/química , Oxidantes/análisis , Fotoquímica , Espectroscopía Infrarroja por Transformada de Fourier , Espectroscopía de Mossbauer , Titanio/química , Difracción de Rayos XRESUMEN
Electrochemical disinfection has gained increasing attention as an alternative for conventional drinking water treatment due to its high effectiveness and environmental compatibility. The most common method of electrochemical disinfection is the use of electro-generated oxidants, such as active chlorine and reactive oxygen species, as disinfectants. This study examined the role of electrode material on the generation of oxidants, and elucidated the different reaction pathways for generating individual oxidants by employing boron-doped diamond (BDD), Ti/RuO(2), Ti/IrO(2), Ti/Pt-IrO(2), and Pt as anode materials. The efficiency of ()OH production, as determined by para-chlorobenzoic acid (pCBA) degradation, was in the order of BDD>>Ti/RuO(2) approximately Pt. No significant production of ()OH was observed at Ti/IrO(2) and Ti/Pt-IrO(2). The ()OH was found to play a key role in O(3) generation at BDD, but not at the other electrodes. The production of active chlorine was in the order of Ti/IrO(2)>Ti/RuO(2)>Ti/Pt-IrO(2)>BDD>Pt. The large difference in this order from that of ROS was attributed to the difference in the electrocatalytic activity of each electrode material toward the production of active chlorine, as evidenced by linear sweep voltammetry (LSV) measurements. In addition, the characteristics of microbial inactivation as a function of electrode material were examined under the presence of an inert electrolyte, using Escherichia coli as an indicator microorganism.
Asunto(s)
Desinfección/métodos , Electroquímica/métodos , Electrodos , Oxidantes/análisis , Cloro/análisis , Diamante , Radical Hidroxilo/análisis , Platino (Metal) , Especies Reactivas de Oxígeno/análisis , TitanioRESUMEN
Cellular systems produce reactive oxygen species during the process of metabolism. Oxidative stress results in the activation or repression of many genes in important signaling pathways. DNA microarrays allow for a high throughput evaluation of the changes in gene expression levels in any biological system. In this study, we describe a method to employ gene expression microarrays to study the transcriptional changes in redox-engineered cell lines that will overexpress MnSOD and/or catalase in the mitochondria.