RESUMEN
OBJECTIVE: This is the first report dealing with immune-mediated inner ear disease (IMIED) hearing loss in a group of patients affected with autoimmune thyroid disease (AITD), whose treatment required corticosteroids, despite being treated with levothyroxine. Immunopathology linking the inner ear and the thyroid gland is also presented. PATIENTS: A total of 220 patients were selected with sensorineural hearing loss (SNHL) of causes other than presbycusis. Audiometry was performed and pure tone average was calculated before and after treatment with corticosteroids. RESULTS: Eighty-four (84) patients had SNHL of autoimmune origin, and 15 patients were diagnosed with AITD (Hashimoto's disease). Bilateral hearing loss was observed in 10 patients (66.5%). Sudden sensorineural hearing loss was the most frequent clinical form of presentation. Nine patients showed a hearing recovery greater than 10dB after corticosteroid treatment. CONCLUSIONS: Acquired hypothyroidism is thought to affect hearing due to different mechanisms. Although specific hormonal therapy may improve peripheral or central auditory disorders associated with hypothyroidism, the presence of IMIED in AITD patients requires another approach. Altered immune regulatory mechanisms involving Treg cells and CD4+CD45RO cells have been suggested in patients with AITD and IMIED. In the present study, although all the patients with hypothyroidism and subclinical hypothyroidism were being treated with levothyroxine, immune-mediated hearing loss was observed. Therapy with corticosteroids could achieve hearing recovery. Since inner ear and thyroid gland share possible antigen targets, we highlight the existence of IMIED in AITD patients and the importance of implementing appropriate therapy with corticosteroids.
Asunto(s)
Pérdida Auditiva Sensorineural/etiología , Tiroiditis Autoinmune/complicaciones , Tiroxina/uso terapéutico , Adulto , Anciano , Especificidad de Anticuerpos , Autoanticuerpos/inmunología , Autoantígenos/inmunología , Linfocitos T CD4-Positivos/inmunología , Oído Interno/inmunología , Femenino , Enfermedad de Hashimoto/complicaciones , Enfermedad de Hashimoto/tratamiento farmacológico , Enfermedad de Hashimoto/inmunología , Pérdida Auditiva Bilateral/etiología , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Bilateral/prevención & control , Pérdida Auditiva Sensorineural/inmunología , Pérdida Auditiva Sensorineural/prevención & control , Pérdida Auditiva Unilateral/etiología , Pérdida Auditiva Unilateral/inmunología , Pérdida Auditiva Unilateral/prevención & control , Humanos , Metilprednisolona/uso terapéutico , Persona de Mediana Edad , Transportadores de Sulfato/inmunología , Subgrupos de Linfocitos T/inmunología , Linfocitos T Reguladores/inmunología , Tiroiditis Autoinmune/tratamiento farmacológicoRESUMEN
Autoimmune inner ear disease is characterized by progressive, bilateral although asymmetric, sensorineural hearing loss. Patients with autoimmune inner ear disease had higher frequencies of interferon-γ-producing T cells than did control subjects tested. Human adipose-derived mesenchymal stem cells (hASCs) were recently found to suppress effector T cells and inflammatory responses and therefore have beneficial effects in various autoimmune diseases. The aim of this study was to examine the immunosuppressive activity of hASCs on autoreactive T cells from the experimental autoimmune hearing loss (EAHL) murine model. Female BALB/c mice underwent ß-tubulin immunization to develop EAHL; mice with EAHL were given hASCs or PBS intraperitoneally once a week for 6 consecutive weeks. Auditory brainstem responses were examined over time. The T helper type 1 (Th1)/Th17-mediated autoreactive responses were examined by determining the proliferative response and cytokine profile of splenocytes stimulated with ß-tubulin. The frequency of regulatory T (Treg) cells and their suppressive capacity on autoreactive T cells were also determined. Systemic infusion of hASCs significantly improved hearing function and protected hair cells in established EAHL. The hASCs decreased the proliferation of antigen-specific Th1/Th17 cells and induced the production of anti-inflammatory cytokine interleukin-10 in splenocytes. They also induced the generation of antigen-specific CD4(+) CD25(+) Foxp3(+) Treg cells with the capacity to suppress autoantigen-specific T-cell responses. The experiment demonstrated that hASCs are one of the important regulators of immune tolerance with the capacity to suppress effector T cells and to induce the generation of antigen-specific Treg cells.
Asunto(s)
Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/terapia , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Bilateral/terapia , Trasplante de Células Madre Mesenquimatosas , Tejido Adiposo/citología , Animales , Enfermedades Autoinmunes/patología , Separación Celular , Potenciales Evocados Auditivos/fisiología , Femenino , Citometría de Flujo , Pérdida Auditiva Bilateral/patología , Humanos , Ratones , Ratones Endogámicos BALB C , Células TH1/inmunología , Células Th17/inmunologíaRESUMEN
Autoimmune inner ear disease (AIED) is a clinical syndrome of uncertain etiology. We present the neuro-otological findings of 2 cases of bilateral hearing loss, dizziness and the antibody profiles of the inner ears. Case 1 had bilateral progressive hearing loss, vestibular dysfunction and abnormal eye movement as the disease progressed. She had inner ear antibodies against 42 and 58kDa protein antigency on Western blot immune assay, and responded to glycocorticosteroid but not to immunosuppressant treatment. Intratympanic steroid injection temporally eliminated her symptoms. However, she developed idiopathic Cushing's syndrome and underwent labyrinthectomy. Case 2 became deaf as a teenager and experienced dizziness 10 years after becoming deaf. He reacted strongly to 68kDa protein and was a good responder to immunosuppressant with steroid. As we still lack a definitive diagnostic test for AIED, careful observation of the clinical course is critical for differential diagnosis regarding the bilateral progressive hearing loss.
Asunto(s)
Autoanticuerpos/sangre , Enfermedades Autoinmunes/inmunología , Oído Interno/inmunología , Pérdida Auditiva Bilateral/inmunología , Enfermedades Vestibulares/inmunología , Adulto , Antiinflamatorios/administración & dosificación , Antiinflamatorios/efectos adversos , Enfermedades Autoinmunes/diagnóstico , Enfermedades Autoinmunes/tratamiento farmacológico , Betametasona/administración & dosificación , Betametasona/efectos adversos , Terapia Combinada , Síndrome de Cushing/inducido químicamente , Relación Dosis-Respuesta a Droga , Esquema de Medicación , Oído Interno/cirugía , Femenino , Estudios de Seguimiento , Gentamicinas/administración & dosificación , Pérdida Auditiva Bilateral/diagnóstico , Pérdida Auditiva Bilateral/tratamiento farmacológico , Humanos , Inmunosupresores/administración & dosificación , Inmunosupresores/efectos adversos , Masculino , Prednisolona/administración & dosificación , Prednisolona/efectos adversos , Enfermedades Vestibulares/diagnóstico , Enfermedades Vestibulares/tratamiento farmacológico , Pruebas de Función Vestibular , Adulto JovenAsunto(s)
Anticuerpos Monoclonales/uso terapéutico , Pérdida Auditiva Bilateral/tratamiento farmacológico , Queratitis/tratamiento farmacológico , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Femenino , Pérdida Auditiva Bilateral/inmunología , Humanos , Infliximab , Queratitis/inmunología , Persona de Mediana Edad , Recuperación de la Función , Inducción de Remisión , SíndromeRESUMEN
A 63-year-old man presented with chronic headache and bilateral hearing loss. A physical examination showed bilateral conjunctivitis. Circulating anti-Cogan peptide antibodies were detected by dot blot analysis. He was diagnosed as having Cogan's syndrome (CS). Steroid therapy led to dramatic improvement of his symptoms and abnormal laboratory findings. During a tapering course of steroid therapy, he suffered from headache. An ophthalmoscopic examination revealed papillary edema. Magnetic resonance imaging of the brain showed hypertrophic cranial pachymeningitis (HCP). After steroid pulse therapy, HCP was improved. To our knowledge, this is the first case of CS complicated with HCP.
Asunto(s)
Enfermedades Autoinmunes/complicaciones , Cefalea/inmunología , Pérdida Auditiva Bilateral/inmunología , Meningitis/complicaciones , Meningitis/inmunología , Cefalea/complicaciones , Cefalea/etiología , Pérdida Auditiva Bilateral/complicaciones , Pérdida Auditiva Bilateral/etiología , Humanos , Masculino , Persona de Mediana Edad , SíndromeAsunto(s)
Anticuerpos Monoclonales/uso terapéutico , Pérdida Auditiva Bilateral , Queratitis/tratamiento farmacológico , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Vértigo/tratamiento farmacológico , Corticoesteroides/uso terapéutico , Adulto , Implantes Cocleares , Femenino , Pérdida Auditiva Bilateral/tratamiento farmacológico , Pérdida Auditiva Bilateral/inmunología , Humanos , Infliximab , Queratitis/inmunología , Síndrome , Vértigo/inmunologíaRESUMEN
Se presenta un breve análisis de la enfermedad autoinmune del oído interno, descrita por primera vez por Mc Cabe. El análisis comprende los aspectos fisiopatológicos, de diagnóstico y tratamiento de esta entidad clínica. Cada aspecto tratado tiene una justificación breve pero precisa de la información bibliográfica existente al momento.
Asunto(s)
Humanos , Enfermedades Autoinmunes/diagnóstico , Enfermedades Autoinmunes/fisiopatología , Enfermedades Autoinmunes/terapia , Enfermedades del Laberinto/diagnóstico , Enfermedades del Laberinto/fisiopatología , Enfermedades del Laberinto/inmunología , Enfermedades del Laberinto/terapia , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Sensorineural/inmunologíaRESUMEN
BACKGROUND: Damage to one inner ear is occasionally followed by contralateral sensorineural hearing loss. This has been defined as sympathetic hearing loss. HYPOTHESIS: It is hypothesized that autoimmunity can play a role in the pathogenesis of sympathetic hearing loss. METHODS: A male patient who developed right-sided sympathetic hearing loss at 20 years of age, 11 years after deafness of the left ear caused by a temporal bone fracture, is described. The patient's serum was analyzed for the presence of autoantibodies against inner ear tissues by immunocytochemistry and Western blotting using rat inner ear tissues. The patient's serum was tested specifically for antibodies against heat shock protein 70 by immunodot blot. The presence of autoantibodies known to play a role in systemic autoimmune disease was also examined. RESULTS: Immunocytochemistry on rat temporal bone sections demonstrated autoantibodies in the patient's serum specifically targeted against cochlear outer hair cells. No reactivity of the patient's serum was observed with control tissues including kidney, brain, and liver. Western blotting using homogenized rat cochlear tissues showed that the patient's serum reacted with a 25- and 27-kDa protein. No reactivity was observed with heat shock protein 70 in the immunodot blot analysis. The patient's serum did not contain autoantibodies against antinuclear antibodies, double-stranded DNA, antineutrophil cytoplasmic antibodies, basement membrane, reticulin, intestinal mucosa, muscle, collagen, or mitochondria. CONCLUSION: Observations indicate that this patient suffered sympathetic hearing loss caused by organospecific autoimmunity directed to cochlear outer hair cells.
Asunto(s)
Enfermedades Autoinmunes/diagnóstico , Pérdida Auditiva Bilateral/diagnóstico , Pérdida Auditiva Sensorineural/diagnóstico , Adulto , Animales , Audiometría de Tonos Puros , Autoanticuerpos/sangre , Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/patología , Western Blotting , Diagnóstico Diferencial , Células Ciliadas Auditivas Externas/inmunología , Células Ciliadas Auditivas Externas/patología , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Bilateral/patología , Pérdida Auditiva Sensorineural/inmunología , Pérdida Auditiva Sensorineural/patología , Humanos , Enfermedades del Complejo Inmune/diagnóstico , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/patología , Técnicas para Inmunoenzimas , Masculino , Ratas , Ratas Wistar , Fracturas Craneales/complicaciones , Hueso Temporal/lesionesAsunto(s)
Rinorrea de Líquido Cefalorraquídeo/etiología , Seudotumor Cerebral/diagnóstico , Acúfeno/etiología , Adulto , Rinorrea de Líquido Cefalorraquídeo/diagnóstico , Femenino , Estudios de Seguimiento , Pérdida Auditiva Bilateral/etiología , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Sensorineural/diagnóstico , Pérdida Auditiva Sensorineural/etiología , Humanos , Obesidad Mórbida/complicaciones , Obesidad Mórbida/terapia , Seudotumor Cerebral/complicaciones , Acúfeno/diagnósticoRESUMEN
In this study, we analyzed the clinical features, diagnostic criteria, treatment, and outcome of atypical bilateral progressive hearing loss associated with myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA). The evaluation was made by audiogram and laboratory data in 6 cases treated at Hokkaido University Hospital. We measured MPO-ANCA by enzyme-linked immunosorbent assay; the result was positive in all cases. The hearing loss developed bilaterally and progressed to total deafness within several weeks. The onset of hearing loss was not simultaneous in the 2 ears. Methylprednisolone pulse therapy was effective in 9 ears. Three ears showed no improvement; 2 of the 3 ears had already exhibited total deafness at the first visit. After the treatment, the titer of MPO-ANCA decreased in all patients. The level of MPO-ANCA should be tested in patients with bilateral progressive hearing loss of unknown origin and can be used as a marker of the disease's activity.
Asunto(s)
Anticuerpos Anticitoplasma de Neutrófilos/inmunología , Enfermedades Autoinmunes/diagnóstico , Pérdida Auditiva Bilateral/diagnóstico , Peroxidasa/inmunología , Adulto , Anciano , Anciano de 80 o más Años , Audiometría de Tonos Puros , Enfermedades Autoinmunes/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Técnica del Anticuerpo Fluorescente Indirecta , Glucocorticoides/uso terapéutico , Pérdida Auditiva Bilateral/inmunología , Humanos , Masculino , Metilprednisolona/uso terapéutico , Persona de Mediana Edad , Resultado del TratamientoRESUMEN
CONTEXT: A number of therapies have been proposed for the long-term management of corticosteroid-responsive, rapidly progressive, bilateral sensorineural hearing loss (autoimmune inner ear disease [AIED]). Methotrexate has emerged as the benchmark agent but has not been rigorously evaluated for hearing improvement in patients with AIED. OBJECTIVE: To assess the efficacy of long-term methotrexate in maintaining hearing improvements achieved with glucocorticoid (prednisone) therapy in patients with AIED. DESIGN, SETTING, AND PARTICIPANTS: A randomized, double-blind, placebo-controlled trial conducted from February 3, 1998, to November 5, 2001, of 67 patients with rapidly progressive, bilateral sensorineural hearing loss at 10 tertiary care centers in the United States. INTERVENTION: Randomization to either oral methotrexate (15 to 20 mg/wk; n = 33) or placebo (n = 34), in combination with an 18-week prednisone taper. Follow-up examinations, including audiometric evaluation, were performed at 4, 8, 12, 24, 36, 48, and 52 weeks, or until hearing loss was documented. MAIN OUTCOME MEASURE: Maintenance of hearing improvement achieved from prednisone treatment. RESULTS: Sixty-seven patients (57.8%) enrolled in the prednisone challenge experienced hearing improvement. Twenty-five patients (37%) experienced hearing improvements in both ears. Of the individuals who reached study end points, 24 (80%) of 30 end points were because of measured hearing loss in the methotrexate group and 29 (93.5%) of 31 end points were because of measured hearing loss in the placebo group (P =.15). Methotrexate was no more effective than placebo in maintaining the hearing improvement achieved with prednisone treatment (hazard ratio, 1.31; 95% confidence interval, 0.79-2.17; P =.30). CONCLUSION: Methotrexate does not appear to be effective in maintaining the hearing improvement achieved with prednisone therapy in patients with AIED.
Asunto(s)
Enfermedades Autoinmunes/tratamiento farmacológico , Enfermedades del Oído/tratamiento farmacológico , Oído Interno , Glucocorticoides/uso terapéutico , Pérdida Auditiva Sensorineural/tratamiento farmacológico , Pérdida Auditiva Sensorineural/inmunología , Inmunosupresores/uso terapéutico , Metotrexato/uso terapéutico , Audiometría , Método Doble Ciego , Pérdida Auditiva Bilateral/tratamiento farmacológico , Pérdida Auditiva Bilateral/inmunología , Humanos , Prednisona/uso terapéutico , Estudios ProspectivosRESUMEN
The audiovestibular system can be affected by an immunologic etiology. The immune-mediated inner ear disease (IMIED) is a syndrome that includes rapidly progressive sensorineural hearing loss, vertigo and tinnitus, which occurs as a primary disorder or complicates certain autoimmune systemic conditions. However, if treated promptly with immunosuppression, the audiological sequel of IMIED may be avoided. We present a 28 year old female patient, who after rhinitis and mioarthralgias developed a vestibular syndrome. A week later she experienced bilateral hearing loss that progressed to deafness in 72 hours. The examination revealed horizontal and torsional nystagmus, a disrupted vestibulo-ocular reflex and vertigo with the positional changes. Laboratory data were normal except for eritrosedimentation rate (75 mm/1 hour). The autoantibodies usually present in rheumatologic autoimmune systemic diseases were negative. The antibodies to the 68-kD antigen found in the inner ear were positive. The chest x-ray and sinus x-ray were normal. The head magnetic resonance imaging with gadolinium and ear computed tomography were normal. Cerebrospinal fluid studies showed normal findings. With the possible diagnosis of IMIED we started early treatment with corticosteroids, with improvement in auditory and vestibular function thereafter. We highlight the early recognition of IMIED as a differential diagnosis in patients with acute bilateral hearing loss, because prompt treatment with immunosuppression might have a positive effect on auditory function recovery.
Asunto(s)
Enfermedades Autoinmunes/complicaciones , Pérdida Auditiva Bilateral/etiología , Pérdida Auditiva Sensorineural/etiología , Enfermedades del Laberinto/complicaciones , Enfermedad Aguda , Adulto , Enfermedades Autoinmunes/diagnóstico , Enfermedades Autoinmunes/inmunología , Diagnóstico Diferencial , Femenino , Pérdida Auditiva Bilateral/diagnóstico , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Sensorineural/diagnóstico , Pérdida Auditiva Sensorineural/inmunología , Humanos , Enfermedades del Laberinto/diagnóstico , Enfermedades del Laberinto/inmunologíaRESUMEN
The audiovestibular system can be affected by an immunologic etiology. The immune-mediated inner ear disease (IMIED) is a syndrome that includes rapidly progressive sensorineural hearing loss, vertigo and tinnitus, which occurs as a primary disorder or complicates certain autoimmune systemic conditions. However, if treated promptly with immunosuppression, the audiological sequel of IMIED may be avoided. We present a 28 year old female patient, who after rhinitis and mioarthralgias developed a vestibular syndrome. A week later she experienced bilateral hearing loss that progressed to deafness in 72 hours. The examination revealed horizontal and torsional nystagmus, a disrupted vestibulo-ocular reflex and vertigo with the positional changes. Laboratory data were normal except for eritrosedimentation rate (75 mm/1 hour). The autoantibodies usually present in rheumatologic autoimmune systemic diseases were negative. The antibodies to the 68-kD antigen found in the inner ear were positive. The chest x-ray and sinus x-ray were normal. The head magnetic resonance imaging with gadolinium and ear computed tomography were normal. Cerebrospinal fluid studies showed normal findings. With the possible diagnosis of IMIED we started early treatment with corticosteroids, with improvement in auditory and vestibular function thereafter. We highlight the early recognition of IMIED as a differential diagnosis in patients with acute bilateral hearing loss, because prompt treatment with immunosuppression might have a positive effect on auditory function recovery. (Au)
Asunto(s)
Humanos , Femenino , Adulto , Pérdida Auditiva Sensorineural/etiología , Pérdida Auditiva Bilateral/etiología , Enfermedades del Laberinto/complicaciones , Enfermedades Autoinmunes/complicaciones , Pérdida Auditiva Sensorineural/inmunología , Pérdida Auditiva Sensorineural/diagnóstico , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Bilateral/diagnóstico , Enfermedades del Laberinto/inmunología , Enfermedades del Laberinto/diagnóstico , Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/diagnóstico , Diagnóstico Diferencial , Enfermedad AgudaRESUMEN
The aim of this study was to evaluate the pathological significance of antibodies against cornea and inner ear tissue in the development of audiovestibular and ocular symptoms in patients with Cogan's syndrome (CS). We analysed the serum of 5 CS patients for binding of IgM and IgG to fresh cryosections of rat labyrinth (semicircular canals, ampulla, utricle, saccule) and cornea by indirect immunofluorescence (IF). The predominant pattern of anti-corneal IgM was staining of the superficial cell layer of the non-keratinizing squamous epithelium. IgM against cornea was found in 3 patients, all of whom had bilateral inflammatory eye signs at the start of the disease. However, IgM was also detected in the chronic stage of the disease when no clinical signs of eye involvement were apparent. The study includes the first follow-up examination of anti-corneal IgM and IgG antibodies during a complete episode of active CS. During the first episode of CS in 1 patient, anti-corneal IgM became detectable 1 week after the onset of interstitial keratitis and 3 weeks after the onset of audiovestibular symptoms. It increased over several weeks and then fell to very low levels. However, at no time was anti-corneal IgG found. In the course of follow-up examinations, the serum of 4 patients intermittently contained low titre IgG antibodies against inner ear labyrinthine tissue, but without any clear correlation with the active stages of CS. In addition, high-resolution MRI (HR-MRI) of the inner ear was performed in the acute and chronic stages of CS to evaluate the activity of CS. In the acute stage, HR-MRI revealed abnormal MRI signals in the vestibule, semicircular canals, vestibular nerve, or cochlea. In the chronic stage, patients showed narrowing or occlusion of semicircular canals and the cochlea on the 3D-CISS images, but no high signal lesions (T1) and no enhancement. Antibodies against cornea or labyrinthine tissue were not consistently detected in CS and the level of organ-specific antibodies did not correlate with the activity of the disease.
Asunto(s)
Anticuerpos/sangre , Córnea/inmunología , Oído Interno/inmunología , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Sensorineural/inmunología , Queratitis/inmunología , Acúfeno/inmunología , Enfermedades Vestibulares/inmunología , Enfermedad Aguda , Adulto , Animales , Enfermedad Crónica , Cóclea/patología , Colorantes , Córnea/patología , Oído Interno/patología , Epitelio/inmunología , Epitelio/patología , Femenino , Técnica del Anticuerpo Fluorescente Indirecta , Estudios de Seguimiento , Pérdida Auditiva Bilateral/patología , Pérdida Auditiva Sensorineural/patología , Humanos , Procesamiento de Imagen Asistido por Computador , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Queratitis/patología , Imagen por Resonancia Magnética , Ratas , Canales Semicirculares/patología , Síndrome , Acúfeno/patología , Enfermedades Vestibulares/patología , Nervio Vestibular/patología , Vestíbulo del Laberinto/patologíaRESUMEN
Although many studies have suggested a relation between allergy and Ménière's disease, the pathophysiology of this condition remains controversial. The aim of this study was to clarify whether an anaphylactic reaction in the inner ear can disturb hearing and equilibrium, and whether such disturbances recur in response to repeated anaphylactic reactions. Increases in audiological threshold, nystagmus, and endolymphatic hydrops were observed in response to a single exposure to antigen administered to actively sensitized guinea pigs. The increase in audiological threshold was maximal 10 h after antigen challenge (p < 0.005) and returned to the baseline level after 7 days. Nystagmus and the increase in audiological threshold induced by antigen exposure were inhibited by prior administration of pemirolast potassium (p < 0.05), an inhibitor of chemical mediator release from mast cells. A second challenge with antigen 7 days after the first also induced an increase in audiological threshold (p < 0.05) and nystagmus. These results suggest that studies of repeated antigen challenge in actively sensitized animal models may increase our understanding of the pathophysiology of Ménière's disease.
Asunto(s)
Anafilaxia/complicaciones , Pérdida Auditiva Bilateral/inmunología , Animales , Cóclea/patología , Dinitrofenoles/inmunología , Oído Interno/inmunología , Hidropesía Endolinfática/etiología , Hidropesía Endolinfática/inmunología , Cobayas , Antagonistas de los Receptores Histamínicos/farmacología , Masculino , Nistagmo Patológico/etiología , Nistagmo Patológico/inmunología , Equilibrio Postural , Piridinas/farmacología , Pirimidinonas/farmacología , VacunaciónAsunto(s)
Pérdida Auditiva Bilateral/tratamiento farmacológico , Pérdida Auditiva Sensorineural/tratamiento farmacológico , Inmunosupresores/uso terapéutico , Metotrexato/uso terapéutico , Adulto , Ciclofosfamida/uso terapéutico , Femenino , Estudios de Seguimiento , Glucocorticoides/uso terapéutico , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Sensorineural/inmunología , Pruebas Auditivas , Humanos , Queratitis/inmunología , Metilprednisolona/uso terapéutico , SíndromeRESUMEN
OBJECTIVE: To identify the 68-kd target of antibody in serum samples from patients with idiopathic, progressive, bilateral sensorineural hearing loss. DESIGN: To purify target protein from renal extracts using gel filtration, ion-exchange chromatography, and polyacrylamide gel electrophoresis and to transfer to nitrocellulose membranes. The purified protein was digested with trypsin, and peptide fragments were separated by high-pressure liquid chromatography. RESULTS: One fraction obtained by high-performance liquid chromatography contained a peptide of 2776 molecular weight. The sequence of a stretch of 22 amino acids within this peptide was identical to that of amino acids 424 through 445 of heat shock protein 70 (HSP70). On Western blotting, monoclonal antibody directed against HSP70 (but capable of recognizing both constitutive HSP70 [HSC70] and stress-inducible HSP70) reacted with the purified 68-kd protein. We compared the reactivity of serum samples from six patients with idiopathic, progressive, bilateral sensorineural hearing loss, as well as monoclonal antibody to HSC70, and monoclonal antibody to HSP70 with renal extract. The pattern obtained suggested that patient antibodies are preferentially directed at HSP70. CONCLUSION: The target of antibody in serum samples from patients with idiopathic, progressive, bilateral sensorineural hearing loss is HSP70.
Asunto(s)
Autoanticuerpos/sangre , Proteínas HSP70 de Choque Térmico/inmunología , Pérdida Auditiva Sensorineural/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales , Western Blotting , Bovinos , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Cromatografía por Intercambio Iónico , Colodión , Electroforesis en Gel de Poliacrilamida , Proteínas HSP70 de Choque Térmico/análisis , Pérdida Auditiva Bilateral/sangre , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Sensorineural/sangre , Humanos , Immunoblotting , Riñón/química , Datos de Secuencia Molecular , Peso Molecular , Fragmentos de Péptidos/análisis , Extractos de Tejidos/análisisRESUMEN
The present paper reports three cases of sensorineural hearing loss of suspected autoimmune origin. Cases 1 and 2 were bilateral sensorineural hearing loss which responded to steroid therapy. Case 3 was bilateral fluctuant sensorineural hearing loss in conjunction with systemic lupus erythematosus. The pathogenesis of autoimmune sensorineural hearing loss is not yet fully understood. In two patients, hearing levels improved or stabilized following the use of osmotic expanders. The clinical results suggest that endolymphatic hydrops may participate in autoimmune sensorineural hearing loss.
Asunto(s)
Enfermedades Autoinmunes/diagnóstico , Pérdida Auditiva Bilateral/diagnóstico , Pérdida Auditiva Sensorineural/diagnóstico , Adulto , Audiometría de Tonos Puros , Autoanticuerpos/sangre , Enfermedades Autoinmunes/tratamiento farmacológico , Enfermedades Autoinmunes/inmunología , Ciclofosfamida/uso terapéutico , Relación Dosis-Respuesta a Droga , Oído Interno/inmunología , Hidropesía Endolinfática/diagnóstico , Hidropesía Endolinfática/tratamiento farmacológico , Hidropesía Endolinfática/inmunología , Femenino , Pérdida Auditiva Bilateral/tratamiento farmacológico , Pérdida Auditiva Bilateral/inmunología , Pérdida Auditiva Sensorineural/tratamiento farmacológico , Pérdida Auditiva Sensorineural/inmunología , Pérdida Auditiva Súbita/diagnóstico , Pérdida Auditiva Súbita/tratamiento farmacológico , Pérdida Auditiva Súbita/inmunología , Humanos , Lupus Eritematoso Sistémico/diagnóstico , Lupus Eritematoso Sistémico/tratamiento farmacológico , Lupus Eritematoso Sistémico/inmunología , Persona de Mediana Edad , Prednisolona/uso terapéuticoRESUMEN
Our aim was to assess whether "idiopathic" bilateral progressive sensorineural hearing loss (BPSHL) has an immunological cause in some patients; antibodies to native type II collagen were sought by an ELISA in eighteen patients with BPSHL, before and after corticosteroid treatment, and in twelve patients with Menière's disease, fifteen with otosclerosis, eighteen with rheumatoid arthritis, nine with fibrositis, and nine healthy controls. A positive result was defined as a mean dilution titre of 2 or more. Eight of eighteen BPSHL patients had positive titres--significantly (p less than 0.005) more than in any other group (one Menière's disease, two otosclerosis, and no others). The mean antibody titre was higher in the BPSHL group than in any other group (2.02 [SEM 0.62] vs 0.17 [0.17]) Menière's disease, 0.44 [0.32] otosclerosis, 0 all others; p less than 0.005). The nine BPSHL patients who showed a clinical response to corticosteroids (improvement in at least one tone by audiogram or 25 db in speech discrimination score) had the highest mean antibody titre (3.46 [0.88] vs 0.59 [0.59] for the nine non-responsive patients; p less than 0.04). We suggest that in some patients with BPSHL, immunity to type II collagen, a major constituent of the inner ear, may be important in the pathogenesis of the disorder.