Seasonal Differences in Contaminant Accumulation in Neotropical Migrant and Resident Songbirds.

For many years, it has been hypothesized that Neotropical migrants breeding in the United States and Canada accumulate organochlorine pesticides (OCPs) while on their wintering grounds in Latin America. We investigated the seasonal accumulation of persistent organic pollutant (POPs) in migrant and resident passerines in Texas, Yucatán, and Costa Rica collected during the fall, winter, and spring from 2011 to 2013. A total of 153 birds were collected, and all contained detectable levels of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and OCPs with dichlorodiphenyldichloroethylene (DDE) being the most predominant pesticide. OCPs and PCBs were the predominant contaminants, accounting for ≥80 % of the total POPs burden, whereas PBDEs accounted for ≤16 %. Only spring migrants from Texas had significantly greater DDE concentrations (64.6 ng/g dry weight [dw]) than migrants collected in Costa Rica (23.2 ng/g dw). Resident birds in Texas had significantly greater levels of DDE (121 ng/g dw) and ΣPBDEs (34.8 ng/g dw) compared with residents in Yucatán and Costa Rica. For ΣPCBs, resident birds from Costa Rica had significantly lower concentrations (9.60 ng/g dw) compared with their migrant counterparts (43.7 ng/g dw) and residents from Texas (48.3 ng/g dw) and the Yucatán (32.1 ng/g dw). Migrant and resident passerines had similar congener profiles for PCBs and PBDEs suggesting similar exposure and retention of these contaminants. No significant accumulation of DDE was observed in migrants while on their wintering grounds. Relatively high concentrations of PBDEs in resident birds from Costa Rica warrant future studies of PBDE contamination in Latin America.

Comparative Study of Lead Concentration in Feathers of Urban and Rural Passerines in Merida, Mexico.

Lead is a commonly monitored heavy metal because of potential health effects on exposed organisms. We quantified lead in secondary feathers of two passerine bird species, clay-colored thrushes (Turdus grayi) and great-tailed grackles (Quiscalus mexicanus), from an urban and a rural site in the municipality of Merida, Yucatan. Urban lead concentration was significantly higher than its rural counterpart for both species (p < 0.05). In the urban site, lead concentration was similar in both species (p = 0.14). However, data from the rural site showed that lead concentration was significantly higher in thrush feathers (p < 0.05). Lead levels herein presented are among the lowest ever reported suggesting that either lead accumulation or absorption is limited. Finally, our data seem to support the hypothesis that species feeding ecology plays a major role in lead accumulation.

Individual inconsistencies in basal and summit metabolic rate highlight flexibility of metabolic performance in a wintering passerine.

Resident passerines inhabiting high latitude environments are faced with strong seasonal changes in thermal conditions and energy availability. Summit metabolic rate (maximal metabolic rate elicited by shivering during cold exposure: M(sum)) and basal metabolic rate (BMR) vary in parallel among seasons and increase in winter due to cold acclimatization, and these adjustments are thought to be critical for survival. Wintering individuals expressing consistently higher M(sum) and BMR could therefore be seen as better performers with higher chances of winter survival than those exhibiting lower metabolic performance. In this study, we calculated repeatability to evaluate temporal consistency of body mass, BMR and M(sum) within and across three consecutives winters in black-capped chickadees (Poecile atricapillus). We found that body mass was significantly repeatable both within and across winters (R 0.51-0.90). BMR (R 0.29-0.47) was only repeatable within winter while M(sum) was repeatable both among (R 0.33-0.49) and within winters (R 0.33-0.49) with the magnitude and significance of repeatability in both variables depending on the year and whether they were corrected for body mass or body size. The patterns of repeatability observed among years also differed between the two variables. Our findings suggest that the relative ranking of individuals in winter metabolic performance is affected by local ecological conditions and can change within relatively short periods of time.

Tolerance to dietary phenolics and diet breadth in three seed-eating birds: implications for Graminivory.

The ability to cope with plant secondary compounds (PSCs) has profound implications for an animal's behavior. In the present study, we assessed the tolerance to dietary phenolics in three seed-eating birds: Zonotrichia capensis, Saltatricula multicolor, and Diuca diuca, which differ in their diet breadth. Seeds in their habitat have distinct chemical composition: grass seeds have less PSCs, specifically, less total phenolics than forb seeds. Based on the detoxification limitation hypothesis and using published data of the natural history of these birds in the central Monte desert, we postulate that predominantly and exclusively graminivorous birds such as D. diuca and S. multicolor, respectively, are less tolerant due, in part, to a lower detoxification capacity than those with greater diet breadth, Z. capensis. To achieve this goal, we measured the food intake of diets varying in their concentration of tannic acid (TA). Indicators of tolerance were body mass change, food, TA and water intake, and glucuronic acid output throughout the experiment. Zonotrichia capensis performed better along the tolerance experiment: it maintained body mass from 0 to 4% TA diet, food and TA intake were higher than the other two species at the end of the experiment, and glucuronic acid output by Z. capensis was greater than D. diuca and S. multicolor from 2% TA diet until the end of the experiment. Our results suggest that Z. capensis is the most tolerant species and this physiological trait may explain their greater diet breadth.

The effects of dietary carotenoid intake on carotenoid accumulation in the retina of a wild bird, the house finch (Carpodacus mexicanus).

Carotenoid pigments accumulate in the retinas of many animals, including humans, where they play an important role in visual health and performance. Recently, birds have emerged as a model system for studying the mechanisms and functions of carotenoid accumulation in the retina. However, these studies have been limited to a small number of domesticated species, and the effects of dietary carotenoid access on retinal carotenoid accumulation have not been investigated in any wild animal species. The purpose of our studies was to examine how variation in dietary carotenoid types and levels affect retinal accumulation in house finches (Carpodacus mexicanus), a common and colorful North American songbird. We carried out three 8-week studies with wild-caught captive birds: (1) we tracked the rate of retinal carotenoid depletion, compared to other body tissues, on a very low-carotenoid diet, (2) we supplemented birds with two common dietary carotenoids (lutein + zeaxanthin) and measured the effect on retinal accumulation, and (3) we separately supplemented birds with high levels of zeaxanthin--an important dietary precursor for retinal carotenoids--or astaxanthin--a dominant retinal carotenoid not commonly found in the diet (i.e. a metabolic derivative). We found that carotenoids depleted slowly from the retina compared to other tissues, with a significant (~50%) decline observed only after 8 weeks on a very low-carotenoid diet. Supplementation with lutein + zeaxanthin or zeaxanthin alone significantly increased only retinal galloxanthin and ε-carotene levels, while other carotenoid types in the retina remained unaffected. Concentrations of retinal astaxanthin were unaffected by direct dietary supplementation with astaxanthin. These results suggest highly specific mechanisms of retinal carotenoid metabolism and accumulation, as well as differential rates of turnover among retinal carotenoid types, all of which have important implications for visual health maintenance and interventions.

Comparative basal metabolic rate among passerines and the food habit hypothesis.

The food habit hypothesis (FHH) predicts that mass-independent BMR is associated with dietary traits. In spite of decades of research, the FHH remains controversial, in part because ambiguities surrounding diet categorization can potentially confound interpretations from interspecific analyses of the relative importance of diet quality. In this study, we investigated possible relationships between BMR and the percentage of nitrogen, and the percentage of various prey items in the gut content of 19 passerine species under an explicit phylogenetic context. There were no significant effects of the percentage of dietary nitrogen, invertebrates, or plants on mass-independent BMR using either conventional or phylogenetically corrected regressions. Consequently, our results do not support the FHH in passerine birds. The lack of a significant correlation between diet and BMR could be explained by generally similar levels of activity and similar body composition among species. In this sense, it is possible that the impact of food habits could depend mainly on body mass, which is also associated to the high cost of the endothermy at small sizes.

Organochlorine pollutants and stable isotopes in resident and migrant passerine birds from northwest Michoacán, Mexico.

Although concentrations of organochlorine compounds (OCs) in birds from most of the United States and Canada have decreased over the last 30 years, there is still concern that migrant birds might be exposed to elevated concentrations of OCs during migration in Latin America. The Lerma-Chapala Basin in west-central Mexico is an important migration corridor and wintering area for many species. The objectives of this study were to assess if resident and migrant birds wintering in western Michoacán, Mexico accumulated elevated concentrations of OCs during fall and spring and to determine if the stable isotopes delta(15)N, delta(13)C, and deltaD could be used to predict burdens and origins of DDE accumulation. Resident and migrant passerine insectivorous birds were collected during fall and spring (2001-2002) in northwest Michoacán, near Chapala Lake, Mexico. The carcasses were analyzed for OCs and tail feathers were analyzed for stable isotopes delta(15)N, delta(13)C, and deltaD. The OCs detected in more than 50% of the samples were: oxychlordane (79%), p,p'-DDE (100%), p,p'-DDT (57%), and total PCBs (100%). p,p'-DDE was the OC detected at the highest concentrations, whereas residues of other OCs were near or below detection limits. Overall, there were no significant differences in concentrations of OCs between seasons or between resident and migrant birds. Concentrations of DDE and oxychlordane were somewhat higher in migrant and resident birds during spring than in fall; however, concentrations were significantly different only for oxychlordane. Two resident birds collected in fall and spring had DDE residues >10 microg/g wet weight in carcass. There were no significant differences in delta(13)C and delta(15)N values among species, between seasons, or between migrant and resident birds. However, deltaD values were clearly different between species and helped differentiate migrant from resident birds. deltaD values also were negatively and significantly correlated with DDE concentrations in carcass. Birds with more depleted deltaD values in feathers tended to have higher DDE concentrations than those with less depleted deltaD values, suggesting a potential latitudinal accumulation of DDE. Overall, our results suggest that during fall and spring, there is not a significant buildup of persistent OCs in migrant and resident passerine insectivorous birds in northwest Michoacán, Mexico.

Variation in maternal effects and embryonic development rates among passerine species.

Embryonic development rates are reflected by the length of incubation period in birds, and these vary substantially among species within and among geographical regions. The incubation periods are consistently shorter in North America (Arizona study site) than in tropical (Venezuela) and subtropical (Argentina) South America based on the study of 83 passerine species in 17 clades. Parents, mothers in particular, may influence incubation periods and resulting offspring quality through proximate pathways, while variation in maternal strategies among species can result from selection by adult and offspring mortality. Parents of long-lived species, as is common in the tropics and subtropics, may be under selection to minimize costs to themselves during incubation. Indeed, time spent incubating is often lower in the tropical and subtropical species than the related north temperate species, causing cooler average egg temperatures in the southern regions. Decreased egg temperatures result in longer incubation periods and reflect a cost imposed on offspring by parents because energy cost to the embryo and risk of offspring predation are both increased. Mothers may adjust egg size and constituents as a means to partially offset such costs. For example, reduced androgen concentrations in egg yolks may slow development rates, but may enhance offspring quality through physiological trade-offs that may be particularly beneficial in longer-lived species, as in the tropics and subtropics. We provide initial data to show that yolks of tropical birds contain substantially lower concentrations of growth-promoting androgens than north temperate relatives. Thus, maternal (and parental) effects on embryonic development rates may include contrasting and complementary proximate influences on offspring quality and deserve further field study among species.

Non-enzymatic lipid peroxidation of microsomes and mitochondria from liver, heart and brain of the bird Lonchura striata: relationship with fatty acid composition.

The aim of this study was to examine the fatty acid composition and non-enzymatic lipid peroxidation (LP) of mitochondria and microsomes obtained from liver, heart and brain of Lonchura striata. The percentage of total unsaturated fatty acid was approximately 30-60% in the organelles from all tissues studied. Brain mitochondria and both organelles of liver exhibited the highest percentage of polyunsaturated fatty acid (PUFA) (30 and 18%, respectively). The arachidonic acid (AA) content was 7% in mitochondria of liver and brain and 3% in heart mitochondria. The percentage of docosahexanoic acid (DHA) was 8% in brain mitochondria and approximately 2-3% in heart and liver mitochondria. The peroxidizability index (PI) of brain mitochondria and both organelles from liver was higher than that of organelles from heart and brain microsomes. Liver organelles and brain mitochondria were affected by LP, as indicated by the increase in chemiluminescence and a decrease of AA and DHA. These changes were not observed during LP of brain microsomes and both organelles from heart. These results indicate: 1) PI positively correlates with PUFA percentage and LP; 2) The resistance to LP detected in heart organelles would contribute to the cardiac protection against oxidative damage.