The Giemsa stain: its history and applications.

Gustav Giemsa was born in Germany in 1867, worked mainly as a chemist, and died in 1948. The staining method, which carries his name, was designed primarily for the demonstration of parasites in malaria, but it was also employed in histology because of the high-quality staining of the chromatin and the nuclear membrane, the metachromasia of some cellular components, and the different qualities of cytoplasmic staining depending on the cell type. The use of methylene azure and its mixture with methylene blue to form an eosinate made stable the stain and its results. Giemsa's stain is regarded as the world's standard diagnostic technique for malaria's plasmodium, and it is also the basic stain for classifying lymphomas in the Kiel classification.

[From miasmata to germs. The impact of bacteriology on medical practice in Canadian territory from 1870 to 1930]. / Entre les miasmes et les germes. L'impact de la bacteriologie sur la pratique medicale en territoire canadien 1879-1930.

This article analyzes some aspects concerning the introduction of bacteriological theory and practice into Canadian territory from 1870 to 1930. The author begins by presenting the principal explanatory models characterizing the transition period from pre-bacteriological discourse--referring to infectious diseases and surgical infections--to new bacteriological paradigms. He goes on to analyze the different ways in which the germ theory and then bacteriology spread into Canadian territory. The transition periods, where old and new coexisted, reveal a kind of syncretism by physicians in relation to both aetiology of infectious diseases and surgical procedures. The author then shows that there were two distinct phases in the overall context of the history of antiseptic surgery from 1868 to 1890. He demonstrates that the use of antiseptics did not necessarily mean either adherence to Pasteur's postulates on fermentation or strict observance of Listerian methods. Finally, the article expounds on the role played by European institutions in importing bacteriological knowledge and institutionalizing this new discipline in Canadian territory.