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1.
J Pharm Sci ; 68(5): 626-8, 1979 May.
Artículo en Inglés | MEDLINE | ID: mdl-430503

RESUMEN

A sensitive and specific radioimmunoassay of dog and human plasma pentazocine is described. Rabbit antiserum and the second antibody method separated bound from free pentazocine. The radioimmunoassay employed an 125I-labeled radioligand and required extraction from the sample prior to quantitation. The method had a detection limit of approximately 200 pg/assay tube (1 ng/ml). The assay was used successfully to measure pentazocine in the plasma of beagle hounds given 0.3 mg of pentazocine/kg iv. The decline in plasma levels fitted a two-compartment body model with a 100-min mean overall half-life and a 3.2-liters/hr mean plasma clearance rate.


Asunto(s)
Pentazocina/sangre , Animales , Especificidad de Anticuerpos , Perros , Estudios de Evaluación como Asunto , Femenino , Humanos , Masculino , Métodos , Modelos Biológicos , Pentazocina/inmunología , Radioinmunoensayo
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