RESUMEN
Fighting cancer still relies on chemo- and radiation therapy, which is a trade-off between effective clearance of malignant cells and severe side effects on healthy tissue. Targeted cancer treatment on the other hand is a promising and refined strategy with less systemic interference. The enzyme horseradish peroxidase (HRP) exhibits cytotoxic effects on cancer cells in combination with indole-3-acetic acid (IAA). However, the plant-derived enzyme is out of bounds for medical purposes due to its foreign glycosylation pattern and resulting rapid clearance and immunogenicity. In this study, we generated recombinant, unglycosylated HRP variants in Escherichia coli using random mutagenesis and investigated their biochemical properties and suitability for cancer treatment. The cytotoxicity of the HRP-IAA enzyme prodrug system was assessed in vitro with HCT-116 human colon, FaDu human nasopharyngeal squamous cell carcinoma and murine colon adenocarcinoma cells (MC38). Extensive cytotoxicity was shown in all three cancer cell lines: the cell viability of HCT-116 and MC38 cells treated with HRP-IAA was below 1% after 24 h incubation and the surviving fraction of FaDu cells was ≤ 10% after 72 h. However, no cytotoxic effect was observed upon in vivo intratumoral application of HRP-IAA on a MC38 tumor model in C57BL/6J mice. However, we expect that targeting of HRP to the tumor by conjugation to specific antibodies or antibody fragments will reduce HRP clearance and thereby enhance therapy efficacy.
Asunto(s)
Antineoplásicos/farmacología , Peroxidasa de Rábano Silvestre/farmacología , Ácidos Indolacéticos/química , Adenocarcinoma/tratamiento farmacológico , Adenocarcinoma/patología , Animales , Antineoplásicos/química , Carcinoma de Células Escamosas/tratamiento farmacológico , Carcinoma de Células Escamosas/patología , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Neoplasias del Colon/tratamiento farmacológico , Neoplasias del Colon/patología , Femenino , Células HCT116 , Peroxidasa de Rábano Silvestre/química , Humanos , Ratones , Ratones Endogámicos C57BL , Neoplasias Nasofaríngeas/tratamiento farmacológico , Neoplasias Nasofaríngeas/patología , ProfármacosRESUMEN
Macromolecular crowding plays an inevitable role in all biological processes influencing association, conformation, and other characteristics of proteins. Present study is based on the effect of macromolecular crowding on structure of horseradish peroxidase (HRP) enzyme. Concentration-dependent conformational changes induced by crowding agents, dextran 70 and polyethylene glycol (PEG)-4000, were monitored employing a range of biophysical techniques. The intrinsic fluorescence spectra showed transition of protein from native to unfolded state. Marked increase in 8-Anilino-1-naphthalene-sulphonoic acid and Thioflavin T fluorescence indicated presence of non-native moieties with 80 mg/mL dextran. Enhanced absorbance in turbidity, Soret, and Congo red in corroboration with scattering intensity at 350nm results revealed incidence of HRP aggregates. A new peak around 218 nm in CD spectra pointed towards change in secondary structure towards ß-sheets. Significant loss of enzyme activity upon structural disruption was seen. Comet assay demonstrated DNA damage and genotoxic nature of HRP aggregates, supporting spectroscopic, and fluorescence results. The normalized results were obtained with 120 mg/mL PEG-4000 close to that of native HRP implying no disruptive effect on structure. It can be hypothesized that macromolecular crowding is a vital element, which can have diverse effects. In this study, dextran 70 was observed to have pro-aggregatory effect while enhanced stability of native enzyme was witnessed with PEG. Hence, it can be stated that PEG has potentially better crowder as it helps retain the native enzyme structure. Routine addition of crowding agents is recommended if biological molecules are to be studied under more physiologically appropriate environments.
Asunto(s)
Dextranos/química , Peroxidasa de Rábano Silvestre/química , Polietilenglicoles/química , Acrilamida/química , Benzotiazoles/química , Dicroismo Circular , Ensayo Cometa , Peroxidasa de Rábano Silvestre/metabolismo , Peroxidasa de Rábano Silvestre/farmacología , Humanos , Linfocitos , Nefelometría y Turbidimetría , Conformación Proteica en Lámina beta , Estabilidad Proteica , Estructura Secundaria de Proteína , Espectrometría de FluorescenciaRESUMEN
Hematin has been used as an alternative enzyme catalyst to horseradish peroxidase (HRP) due to its iron-containing activity center. Although hematin and it derivatives have been widely used for polymerization of phenol/analine compounds, it has some drawbacks such as the limited solubility and reaction only at high pH condition. Herein, we report a nanosized biomimetic catalyst, hematin-decorated polyamidoamine dendrimer (G3.0-He) that can effectively catalyze the in situ hydrogelation of phenol-conjugated polymers under neutral pH condition. We demonstrate that G3.0-He particles are smaller than 100 nm and have excellent enzyme-mimetic functions. Interestingly, the nanosized catalyst is not inactivated at high H2O2 concentration. Compared to pure hematin, G3.0-He has significantly higher dispersion in acidic and neutral media, and preserves the percentage of survival of fibroblasts over 90%. Notably, G3.0-He possesses an exquisite HRP-mimicking activity in gelation of gelatin derivative with phenolic hydroxyl (tyamine) moieties under mild physiological conditions. The in vitro study demonstrated that Gel-Tyr hydrogel by G3.0-He catalyzed reaction had excellent cytocompatibility and an excellent scaffold for adhesion to fibroblast cells. Therefore, the designed minimalistic G3.0-He catalyst could serve as an effective catalytic alternative for HRP enzyme in the preparation of biomedical hydrogels.
Asunto(s)
Materiales Biomiméticos , Dendrímeros , Fibroblastos/metabolismo , Hemina , Ensayo de Materiales , Nanopartículas/química , Materiales Biomiméticos/química , Materiales Biomiméticos/farmacología , Línea Celular , Dendrímeros/química , Dendrímeros/farmacología , Hemina/química , Hemina/farmacología , Peroxidasa de Rábano Silvestre/química , Peroxidasa de Rábano Silvestre/farmacología , HumanosRESUMEN
Current hyaluronic acid-based hydrogels often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective biomedical and tissue engineering applications. Provision of the cell-adhesive surface is an important requirement to improve its biocompatibility. An aqueous solution of hyaluronic acid possessing phenolic hydroxyl (HA-Ph) moieties is gellable via a horseradish peroxidase (HRP)-catalyzed oxidative cross-linking reaction. This study evaluates the effect of different degrees of cross-linked Ph moieties on cellular adhesiveness and proliferation on the resultant enzymatically cross-linked HA-Ph hydrogels. Mechanical characterization demonstrated that the compression force of engineered hydrogels could be tuned in the range of 0.05-35 N by changing conjugated Ph moieties in the precursor formulation. The water contact angle and water content show hydrophobicity of hydrogels increased with increasing content of cross-linked Ph groups. The seeded mouse embryo fibroblast-like cell line and human cervical cancer cell line, on the HA-Ph hydrogel, proved cell attachment and spreading with a high content of cross-linked Ph groups. The HA-Ph with a higher degree of Ph moieties shows the maximum degree of cell adhesion, spreading, and proliferation which presents this hydrogel as a suitable biomaterial for biomedical and tissue engineering applications.
Asunto(s)
Hidrogeles/farmacología , Fenol/farmacología , Animales , Adhesión Celular , Encapsulación Celular , Línea Celular , Fuerza Compresiva , Reactivos de Enlaces Cruzados , Femenino , Fibroblastos , Células HeLa , Peroxidasa de Rábano Silvestre/farmacología , Humanos , Ácido Hialurónico/química , Interacciones Hidrofóbicas e Hidrofílicas , Pruebas Mecánicas , Ratones , Agua , Soporte de PesoRESUMEN
The removal of lignin is important to the recovery of saccharides from the pre-hydrolysis liquor (PHL) in kraft-based dissolved pulp production. A one-step process for lignin removal from PHL via treatment with horseradish peroxidase (HRP) in the presence of Ca2+ was proposed, and its principle was studied. The results demonstrated synergy between HRP and Ca2+ in lignin removal from PHL, whereas NH4+ had little effect on lignin removal. HRP treatment in the presence of 60 mmol/L of Ca2+ resulted in a lignin removal of 64.8% accompanied by a saccharide loss of 14.2%. HRP catalyzed both the polymerization and depolymerization of the lignin in the PHL. The HRP-catalyzed lignin polymerization rendered some lignin insoluble enabling it to be directly removed. The HRP-catalyzed depolymerization of lignin decreased its molecular weight with an evident increase in its carboxyl content. The insoluble complexes formed between the lignin with carboxyl and the Ca2+ facilitated the removal of the depolymerized lignin.
Asunto(s)
Peroxidasa de Rábano Silvestre/farmacología , Hidrólisis/efectos de los fármacos , Lignina/aislamiento & purificación , Azúcares/química , Calcio/química , Peroxidasa de Rábano Silvestre/química , Lignina/químicaRESUMEN
An Auxiliary Activity Family 5 (AA5) copper-radical alcohol oxidase (AlcOx) with promiscuous activity towards simple alkyl and aromatic alcohols was evaluated using real-time reaction progress monitoring. Reaction kinetics using variable time normalization analysis (VTNA) were determined from reaction progress curves. By this approach, a detailed view of the entire reaction time course under various conditions was obtained and used to identify parameters that will inform further process optimization development. Optimal activity was found impacted by several factors, including reaction pH, oxygen saturation, and the source of a co-oxidant, either HRP or a chemical alternative, potassium ferricyanide. Analysis of reaction progress curves demonstrated that reaction stalling occurred as a result of oxygen depletion and from a loss of enzyme activity over time. The cooperativity between AlcOx, horseradish peroxidase (HRP), and catalase that result in enhanced reactivity was explored, with reaction pH being identified as a key factor for optimal activity. The results show that a process with HRP is more robust than with potassium ferricyanide, but that both oxidants likely activate AlcOx by a similar mechanism. The phenomenon of product inhibition was investigated for representative reactants, revealing that reaction inhibition was more significant for butyraldehyde than for benzaldehyde. Our analysis suggests that this is linked to the greater proportion in which butyraldehyde exists in the hydrated form.
Asunto(s)
Oxidorreductasas de Alcohol/metabolismo , Biocatálisis , Aldehídos , Catalasa/farmacología , Cobre , Peroxidasa de Rábano Silvestre/farmacología , Cinética , Oxidantes/farmacología , Oxígeno/metabolismo , Oxígeno/farmacologíaRESUMEN
Enzymatic crosslinking chemistry using horseradish peroxidase (HRP) has been widely utilized as an effective approach to fabricating injectable hydrogels with high efficiency under mild reaction conditions. However, their clinical applications are limited by the immunogenicity of the plant-derived enzyme. Herein we report the design, synthesis and characterization of HRP-immobilized porous silica particles (HRP-particles) and their use for in situ formation of HRP-free hydrogels. HRP was immobilized on aminopropyl-modified porous silica particles of 70-140⯵m in diameter via poly(ethylene glycol) spacers of different molecular weights by reductive amination reaction. Two different HRP-free hydrogels based on dextran-tyramine and gelatin-hydroxyphenylpropionic acid (GHPA) conjugates were produced by passing a solution containing the conjugates and H2O2 through a syringe packed with HRP-particles. The storage modulus and gelation rate of both hydrogels were tunable by varying the contact time between the polymer solution and HRP-particles. Our in vitro study revealed that HRP-free GHPA hydrogel was less stimulatory to activated mouse macrophages than HRP-containing GHPA hydrogel with the same stiffness. Furthermore, HRP-free GHPA hydrogel exhibited remarkably lower levels of local and systemic inflammation than HRP-containing one upon subcutaneous injection in immunocompetent C57BL/6J mice. The attenuated immunogenicity of HRP-free GHPA hydrogels makes them an attractive platform for tissue engineering applications. STATEMENT OF SIGNIFICANCE: The immunogenicity of HRP is a significant issue for clinical application of HRP-catalyzed in situ forming hydrogels. HRP-particles are developed to overcome the safety concerns by fabricating HRP-free hydrogels. The porosity of silica particles and molecular weight of poly(ethylene glycol) spacers are discovered as important factors determining the catalytic ability of HRP-particles to induce the in situ crosslinking of polymer-phenol conjugates. Although several articles speculate the potential of HRP to trigger immune responses when administered as a part of hydrogel formulation, no literature has attempted to investigate the immunogenicity of HRP-containing hydrogels in comparison with HRP-free hydrogels. Our findings suggest that the immunogenicity issue should be carefully considered before clinical translation of HRP-containing hydrogels.
Asunto(s)
Hidrogeles , Ingeniería de Tejidos , Animales , Enzimas Inmovilizadas/química , Enzimas Inmovilizadas/inmunología , Enzimas Inmovilizadas/farmacología , Peroxidasa de Rábano Silvestre/química , Peroxidasa de Rábano Silvestre/inmunología , Peroxidasa de Rábano Silvestre/farmacología , Células Endoteliales de la Vena Umbilical Humana , Humanos , Hidrogeles/química , Hidrogeles/farmacología , Peróxido de Hidrógeno/química , Peróxido de Hidrógeno/inmunología , Peróxido de Hidrógeno/farmacología , Masculino , Ratones , Porosidad , Células RAW 264.7RESUMEN
Hydrogen peroxide (H2O2) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.
Asunto(s)
Peróxido de Hidrógeno/análisis , Polyporales/metabolismo , Madera/microbiología , Ampirona/química , Ampirona/farmacología , Benzotiazoles/química , Benzotiazoles/farmacología , Pared Celular/efectos de los fármacos , Celulosa/química , Celulosa/metabolismo , Peroxidasa de Rábano Silvestre/química , Peroxidasa de Rábano Silvestre/farmacología , Peróxido de Hidrógeno/química , Peróxido de Hidrógeno/metabolismo , Límite de Detección , Oxazinas/química , Oxazinas/farmacología , Fenoles/química , Fenoles/farmacología , Polyporales/química , Especies Reactivas de Oxígeno/química , Especies Reactivas de Oxígeno/metabolismo , Ácidos Sulfónicos/química , Ácidos Sulfónicos/farmacología , Sulfóxidos/química , Sulfóxidos/farmacologíaRESUMEN
The ELISA is recognized as an efficient diagnostic tool for antibody detection, but there is no standard ELISA assay for detection of antibodies against hemorrhagic septicemia (HS) in cattle. The present study reports on an indirect ELISA assay for antibody detection of HS in dairy cows, and evaluates the sensitivity (Se) and specificity (Sp) of the method using a Bayesian approach. An indirect ELISA was developed with two types of heat extract antigens, Pasteurella multocida strains P-1256 and M-1404, as coating antigens. A checkerboard titration was employed using dairy cow sera immunized with P. multocida bacterin and colostrum-deprived calf sera. The concentrations of heat extract antigen (160µg/mL), sample serum (1:100) and goat anti-bovine immunoglobulin G labeled with horseradish peroxidase (1:2000) were optimal for the assay. The cut-off values were 0.147 and 0.128 for P-1256 and M-1404 coating antigens, and there were no differences in the results of tests with positive and negative sera (p<0.05). The characteristics of three diagnostic tests were evaluated using a one-population Bayesian model, assuming conditional dependence between two types of coating antigen-based ELISAs and indirect hemagglutination assay (IHA). A total of 415 sera samples from dairy cows without HS vaccination and no history of disease were tested. The Se and Sp of the P-1256 and M-1404 ELISAs were higher than those of the IHA. The Se and Sp of the P-1256 ELISA were 90.3% and 90.1%, while the Se and Sp of the M-1404 ELISA were 92.1% and 71.9%. The median values of Se and Sp from the IHA were 36.0% and 58.2%.
Asunto(s)
Anticuerpos Antibacterianos/análisis , Pruebas de Enzimas/métodos , Septicemia Hemorrágica/diagnóstico , Inmunoglobulina G/inmunología , Pasteurella multocida/inmunología , Animales , Anticuerpos Antibacterianos/inmunología , Teorema de Bayes , Bovinos , Pruebas de Enzimas/veterinaria , Pruebas de Hemaglutinación/veterinaria , Septicemia Hemorrágica/veterinaria , Peroxidasa de Rábano Silvestre/inmunología , Peroxidasa de Rábano Silvestre/farmacología , Inmunoglobulina G/análisis , Sensibilidad y Especificidad , Suero/inmunologíaRESUMEN
Cancer is a major cause of death. Common chemo- and radiation-therapies damage healthy tissue and cause painful side effects. The enzyme horseradish peroxidase (HRP) has been shown to activate the plant hormone indole-3-acetic acid (IAA) to a powerful anticancer agent in in vitro studies, but gene directed enzyme prodrug therapy (GDEPT) studies showed ambivalent results. Thus, HRP/IAA in antibody directed enzyme prodrug therapy (ADEPT) was investigated as an alternative. However, this approach has not been intensively studied, since the enzyme preparation from plant describes an undefined mixture of isoenzymes with a heterogenic glycosylation pattern incompatible with the human system. Here, we describe the recombinant production of the two HRP isoenzymes C1A and A2A in a Pichia pastoris benchmark strain and a glyco-engineered strain with a knockout of the α-1,6-mannosyltransferase (OCH1) responsible for hypermannosylation. We biochemically characterized the enzyme variants, tested them with IAA and applied them on cancer cells. In the absence of H2 O2 , HRP C1A turned out to be highly active with IAA, independent of its surface glycosylation. Subsequent in vitro cytotoxicity studies with human T24 bladder carcinoma and MDA-MB-231 breast carcinoma cells underlined the applicability of recombinant HRP C1A with reduced surface glycoslyation for targeted cancer treatment. Summarizing, this is the first study describing the successful use of recombinantly produced HRP for targeted cancer treatment. Our findings might pave the way for an increased use of the powerful isoenzyme HRP C1A in cancer research in the future.
Asunto(s)
Antineoplásicos/farmacología , Peroxidasa de Rábano Silvestre/farmacología , Profármacos , Proteínas Recombinantes/farmacología , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Peroxidasa de Rábano Silvestre/química , Peroxidasa de Rábano Silvestre/aislamiento & purificación , Humanos , Ácidos Indolacéticos/química , Concentración 50 Inhibidora , Isoenzimas , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificaciónRESUMEN
To investigate the feedback effect from area 7 to areas 17 and 18, intrinsic signal optical imaging combined with pharmacological, morphological methods and functional magnetic resonance imaging (fMRI) was employed. A spatial frequency-dependent decrease in response amplitude of orientation maps was observed in areas 17 and 18 when area 7 was inactivated by a local injection of GABA, or by a lesion induced by liquid nitrogen freezing. The pattern of orientation maps of areas 17 and 18 after the inactivation of area 7, if they were not totally blurred, paralleled the normal one. In morphological experiments, after one point at the shallow layers within the center of the cat's orientation column of area 17 was injected electrophoretically with HRP (horseradish peroxidase), three sequential patches in layers 1, 2 and 3 of area 7 were observed. Employing fMRI it was found that area 7 feedbacks mainly to areas 17 and 18 on ipsilateral hemisphere. Therefore, our conclusions are: (1) feedback from area 7 to areas 17 and 18 is spatial frequency modulated; (2) feedback from area 7 to areas 17 and 18 occurs mainly ipsilaterally; (3) histological feedback pattern from area 7 to area 17 is weblike.
Asunto(s)
Retroalimentación Sensorial/fisiología , Red Nerviosa/fisiología , Corteza Visual/fisiología , Animales , Gatos , Criocirugía , Retroalimentación Sensorial/efectos de los fármacos , Peroxidasa de Rábano Silvestre/administración & dosificación , Peroxidasa de Rábano Silvestre/farmacología , Imagen por Resonancia Magnética , Red Nerviosa/efectos de los fármacos , Red Nerviosa/lesiones , Imagen Óptica , Corteza Visual/efectos de los fármacos , Corteza Visual/lesiones , Ácido gamma-Aminobutírico/administración & dosificación , Ácido gamma-Aminobutírico/farmacologíaRESUMEN
A new glucose-driven photodynamic antimicrobial system was developed to efficiently kill bacteria and fungi, taking advantage of organic-inorganic network materials encapsulating glucose oxidase and horseradish peroxidase and bioluminescence resonance energy transfer (BRET).
Asunto(s)
Antiinfecciosos/administración & dosificación , Gadolinio/química , Glucosa Oxidasa/administración & dosificación , Glucosa/metabolismo , Peroxidasa de Rábano Silvestre/administración & dosificación , Peróxido de Hidrógeno/metabolismo , Compuestos Organometálicos/química , Adenosina Monofosfato/metabolismo , Antiinfecciosos/farmacología , Candida albicans/efectos de los fármacos , Candidiasis/tratamiento farmacológico , Escherichia coli/efectos de los fármacos , Infecciones por Escherichia coli/tratamiento farmacológico , Glucosa Oxidasa/farmacología , Peroxidasa de Rábano Silvestre/farmacología , Humanos , Luz , Luminiscencia , Modelos Moleculares , FotoquimioterapiaRESUMEN
We investigated the toxic effects on Prototheca zopfii of indole-3-acetic acid (IAA) and 2,4-pentanedione (PD) combined with horseradish peroxidase (HRP) alongside the oxidation products of 3-methyl-2-oxindole (MOI) and indole-3-carbinol (I3C) from the IAA/HRP system and methylglyoxal (MGO) from the PD/HRP system. The microorganism was incubated in the absence (control) or presence of IAA, PD, IAA/HRP, PD/HRP, MOI, I3C and MGO and determined: (1) cytotoxicity by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay; (2) growth inhibitory concentration by resazurin assay and (3) antioxidant enzymes activities of: catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD). P. zopfii was more susceptible to IAA at 40 mM than PD at the same concentration, which seems to indicate that IAA was more effective at initiating cell death. These data corroborate results from the resazurin assay. Concentrations of 40 mM of IAA, IAA/HRP and PD/HRP, 20 mM of PD/HRP, 10 mM of MOI, 2 mM of I3C and 8 mM of MGO inhibited the growth of P. zopfii. With sub-inhibitory concentrations of IAA and IAA/HRP at 30 mM, MOI at 8 mM and I3C at 1 mM, the activities of CAT and GR increased, whereas no statistical difference was observed for CAT activity with IAA/HRP. Thus, PD at 30 mM and MGO at 6 mM increased the activities of CAT and GR, whereas PD/HRP system at 15 mM decreased CAT activity and PD/HRP and MGO showed no statistical difference for SOD activity. In conclusion, IAA/HRP or PD/HRP systems and their oxidation products exert cytotoxic effects on P. zopffi; however, I3C and MGO appear to exert greater microbicidal effect on P. zopfii.
Asunto(s)
Catalasa/metabolismo , Glutatión Reductasa/metabolismo , Oxidantes/farmacología , Estrés Oxidativo/efectos de los fármacos , Prototheca/metabolismo , Superóxido Dismutasa/metabolismo , Antioxidantes/metabolismo , Peroxidasa de Rábano Silvestre/farmacología , Ácidos Indolacéticos/farmacología , Indoles/farmacología , Estrés Oxidativo/fisiología , Oxindoles , Pentanonas/farmacología , Prototheca/enzimología , Piruvaldehído/farmacologíaRESUMEN
The features of distribution and morphological structure of the motor cortex neuronal populations projecting to the cerebellar-recipient ventrolateral nucleus of the thalamus after its partial deafferentation were studied in adult cats. The partial deafferentation of the ventrolateral nucleus was evoked by preliminary (three months) electrolytic destruction of the contralateral interpositus nucleus of the cerebellum. The method of retrograde axonal transport with local introductions of the marker was used. All labeled neurons were presented by populations of non-pyramidal neurons and small and medium-sized pyramids, which were distributed in the deep cortical layers: in a lower layer division of V and mostly in layer VI. The labeled neurons were observed in cortical fields 4γ and field 6αß. The data obtained showed no structural reorganization of cortical projections to the deafferented ventrolateral nucleus of the thalamus. It is assumed that this is due to the high degree of specialization of the studied system, triggering the motor program. Neuroplastic changes manifested in the abnormal transformation of proximal portions of dendrites and presence of a large number of "paired" pyramidal neurons compared to intact animals.
Asunto(s)
Cerebelo/ultraestructura , Bulbo Raquídeo/ultraestructura , Corteza Motora/ultraestructura , Neuronas/ultraestructura , Núcleos Talámicos Ventrales/ultraestructura , Animales , Transporte Axonal , Gatos , Cerebelo/efectos de los fármacos , Cerebelo/metabolismo , Peroxidasa de Rábano Silvestre/metabolismo , Peroxidasa de Rábano Silvestre/farmacología , Bulbo Raquídeo/efectos de los fármacos , Bulbo Raquídeo/metabolismo , Corteza Motora/efectos de los fármacos , Corteza Motora/metabolismo , Plasticidad Neuronal , Neuronas/clasificación , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Técnicas Estereotáxicas , Transmisión Sináptica , Núcleos Talámicos Ventrales/efectos de los fármacos , Núcleos Talámicos Ventrales/lesiones , Núcleos Talámicos Ventrales/metabolismoRESUMEN
The enzymatic polymerization of aniline to polyaniline (PANI) with Trametes versicolor laccase (TvL) as catalyst and dioxygen (O2) as oxidant was investigated in an aqueous medium containing unilamellar vesicles with an average diameter of about 80 nm formed from AOT (=sodium bis(2-ethylhexyl) sulfosuccinate). Compared to the same reaction carried out with horseradish peroxidase isoenzyme C (HRPC) as catalyst and hydrogen peroxide (H2O2) as oxidant, notable differences were found in the kinetics of the reaction, as well as in the characteristics of the PANI obtained. Under comparable optimal conditions, which are pH 3.5 for TvL/O2 and pH 4.3 for HRPC/H2O2, the reaction with TvL/O2 was much slower than with HRPC/H2O2, i.e. ≈27 days vs. 1 day reaction time to reach equilibrium with >90% yield at 25 °C. Although in both cases, aniline monomer coupling occurred mainly via the carbon atom in para position of aniline, UV-vis-NIR absorption and EPR measurements indicate that the reaction with TvL/O2 yielded mainly overoxidized products (with λ(max)=730 nm). These products had a lower amount of unpaired electrons if compared with the products obtained with HRPC/H2O2 (with λ(max)≈1000 nm, which is characteristic for the polaron state of PANI-ES, the emeraldine salt form of PANI). Similarly to previous findings with HRPC/H2O2, enzyme inactivation occurred during the polymerization also in the case of TvL/O2. Since the aqueous PANI-vesicle suspensions obtained are of high colloidal stability, they can be used directly as ink in a conventional thermal inkjet printer for printing on paper or on surface treated polyimide films. Printed PANI-ES patterns on paper changed colour from green (emeraldine salt) to blue (emeraldine base) upon exposure to ammonia gas, demonstrating the expected ammonia sensing properties.
Asunto(s)
Compuestos de Anilina/química , Proteínas Fúngicas/metabolismo , Lacasa/metabolismo , Trametes/enzimología , Liposomas Unilamelares , Amoníaco/farmacología , Biocatálisis , Color , Espectroscopía de Resonancia por Spin del Electrón , Peroxidasa de Rábano Silvestre/farmacología , Peróxido de Hidrógeno/química , Tinta , Cinética , Estructura Molecular , Oxidantes/farmacología , Oxidación-Reducción , Oxígeno/farmacología , Polimerizacion , Impresión , Espectrofotometría Atómica , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja CortaRESUMEN
The usefulness of cell-enclosing microcapsules in biomedical and biopharmaceutical fields is widely recognized. In this study, we developed a method enabling the preparation of microcapsules with a liquid core in one step using two enzymatic reactions, both of which consume H2 O2 competitively. The microcapsule membrane prepared in this study is composed of the hydrogel obtained from an alginate derivative possessing phenolic hydroxyl moieties (Alg-Ph). The cell-enclosing microcapsules with a hollow core were obtained by extruding an aqueous solution of Alg-Ph containing horseradish peroxidase (HRP), catalase, and cells into a co-flowing stream of liquid paraffin containing H2 O2 . Formation of the microcapsule membrane progressed from the surface of the droplets through HRP-catalyzed cross-linking of Ph moieties by consuming H2 O2 supplied from the ambient liquid paraffin. A hollow core structure was induced by catalase-catalyzed decomposition of H2 O2 resulting in the center region being at an insufficient level of H2 O2 . The viability of HeLa cells was 93.1% immediately after encapsulation in the microcapsules with about 250 µm diameter obtained from an aqueous solution of 2.5% (w/v) Alg-Ph, 100 units mL(-1) HRP, 9.1 × 10(4) units mL(-1) catalase. The enclosed cells grew much faster than those in the microparticles with a solid core. In addition, the thickness of microcapsule membrane could be controlled by changing the concentrations of HRP and catalase in the range of 13-48 µm. The proposed method could be versatile for preparing the microcapsules from the other polymer derivatives of carboxymetylcellulose and gelatin.
Asunto(s)
Cápsulas/química , Catalasa/química , Peroxidasa de Rábano Silvestre/química , Peróxido de Hidrógeno/química , Alginatos/química , Biofarmacia , Cápsulas/farmacología , Catalasa/farmacología , Adhesión Celular/efectos de los fármacos , Técnicas de Cultivo de Célula , Proliferación Celular/efectos de los fármacos , Ácido Glucurónico/química , Células HeLa , Ácidos Hexurónicos/química , Peroxidasa de Rábano Silvestre/farmacología , Humanos , Hidrogel de Polietilenoglicol-Dimetacrilato/química , Hidrogel de Polietilenoglicol-Dimetacrilato/farmacología , Peróxido de Hidrógeno/farmacologíaRESUMEN
Activity-dependent bulk endocytosis is the dominant synaptic vesicle retrieval mode during high intensity stimulation in central nerve terminals. A key event in this endocytosis mode is the generation of new vesicles from bulk endosomes, which replenish the reserve vesicle pool. We have identified an essential requirement for both adaptor protein complexes 1 and 3 in this process by employing morphological and optical tracking of bulk endosome-derived synaptic vesicles in rat primary neuronal cultures. We show that brefeldin A inhibits synaptic vesicle generation from bulk endosomes and that both brefeldin A knockdown and shRNA knockdown of either adaptor protein 1 or 3 subunits inhibit reserve pool replenishment from bulk endosomes. Conversely, no plasma membrane function was found for adaptor protein 1 or 3 in either bulk endosome formation or clathrin-mediated endocytosis. Simultaneous knockdown of both adaptor proteins 1 and 3 indicated that they generated the same population of synaptic vesicles. Thus, adaptor protein complexes 1 and 3 play an essential dual role in generation of synaptic vesicles during activity-dependent bulk endocytosis.
Asunto(s)
Complejo 1 de Proteína Adaptadora/metabolismo , Complejo 3 de Proteína Adaptadora/metabolismo , Endosomas/fisiología , Neuronas/citología , Vesículas Sinápticas/metabolismo , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/genética , Complejo 1 de Proteína Adaptadora/genética , Complejo 3 de Proteína Adaptadora/genética , Animales , Animales Recién Nacidos , Brefeldino A/farmacología , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Células Cultivadas , Cerebelo/citología , Dextranos/metabolismo , Endosomas/efectos de los fármacos , Exocitosis/efectos de los fármacos , Exocitosis/genética , Femenino , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Peroxidasa de Rábano Silvestre/farmacología , Masculino , Neuronas/efectos de los fármacos , Técnicas de Placa-Clamp , Cloruro de Potasio/farmacología , Inhibidores de la Síntesis de la Proteína/farmacología , ARN Interferente Pequeño/metabolismo , Ratas , Rodaminas/metabolismo , Vesículas Sinápticas/efectos de los fármacos , Transfección/métodosRESUMEN
OBJECTIVE: To culture equine myoblasts from muscle microbiopsy specimens, examine myoblast production of reactive oxygen species (ROS) in conditions of anoxia followed by reoxygenation, and assess the effects of horseradish peroxidase (HRP) and myeloperoxidase (MPO) on ROS production. ANIMALS: 5 healthy horses (5 to 15 years old). PROCEDURES: Equine skeletal myoblast cultures were derived from 1 or 2 microbiopsy specimens obtained from a triceps brachii muscle of each horse. Cultured myoblasts were exposed to conditions of anoxia followed by reoxygenation or to conditions of normoxia (control cells). Cell production of ROS in the presence or absence of HRP or MPO was assessed by use of a gas chromatography method, after which cells were treated with a 3,3'-diaminobenzidine chromogen solution to detect peroxidase binding. RESULTS: Equine skeletal myoblasts were successfully cultured from microbiopsy specimens. In response to anoxia and reoxygenation, ROS production of myoblasts increased by 71%, compared with that of control cells. When experiments were performed in the presence of HRP or MPO, ROS production in myoblasts exposed to anoxia and reoxygenation was increased by 228% and 183%, respectively, compared with findings for control cells. Chromogen reaction revealed a close adherence of peroxidases to cells, even after several washes. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that equine skeletal myoblast cultures can be generated from muscle microbiopsy specimens. Anoxia-reoxygenation-treated myoblasts produced ROS, and production was enhanced in the presence of peroxidases. This experimental model could be used to study the damaging effect of exercise on muscles in athletic horses.
Asunto(s)
Enfermedades de los Caballos/patología , Hipoxia/veterinaria , Fibras Musculares Esqueléticas/metabolismo , Proteínas Musculares/metabolismo , Mioblastos Esqueléticos/metabolismo , Oxígeno/farmacología , Especies Reactivas de Oxígeno/metabolismo , Animales , Cromatografía de Gases/veterinaria , Peroxidasa de Rábano Silvestre/metabolismo , Peroxidasa de Rábano Silvestre/farmacología , Caballos , Hipoxia/patología , Fibras Musculares Esqueléticas/citología , Mioblastos Esqueléticos/citología , Peroxidasa/metabolismo , Peroxidasa/farmacologíaRESUMEN
Using cortico-cortical connection analysis technique, the cat visual area PMLS (the area located on posterior medial wall of the lateral suprasylvian sulcus) retinotopic organization was investigated. A retrograde axonal tracer: horseradish peroxidase (HRP), was injected in the PMLS, and initial neurons were investigated in area 17. It was shown that after HRP injection in PMLS locus, where a central vision field is located, a labelled cell pattern in area 17 corresponded to the L. Palmer et al., 1978, retinotopic map. On the contrary, after HRP injection in PMLS locus, where an upper vision field must be located, as L. Palmer et al., 1978 predicted, initial neurons are visualized in area 17 loci where low visual periphery is displayed: -10 degrees to -60 degrees in vertical meridian and 40 degrees to 80 degrees in horizontal meridian. Such discrepancy in upper and lower visual field representation was also obtained in electrophysiological and topographic investigations by Grant, Shipp, 1991. The data suggest necessity of S. Grant and S. Shipp's retinotopic map use in the cat area PMLS morphofunctional investigation.