RESUMEN
Brucellosis, a zoonotic disease caused by Brucella species, poses a significant global health concern. Among its diverse clinical manifestations, neurobrucellosis remains an infrequent yet debilitating complication. Here, we present a rare case of neurobrucellosis with unusual presentations in a 45-year-old woman. The patient's clinical course included progressive lower extremity weakness, muscle wasting, and double vision, prompting a comprehensive diagnostic evaluation. Notable findings included polyneuropathy, elevated brucella agglutination titers in both cerebrospinal fluid and blood, abnormal EMG-NCV tests, and resolving symptoms with antibiotic therapy. The clinical presentation, diagnostic challenges, and differentiation from other neurological conditions are discussed. This case underscores the importance of considering neurobrucellosis in regions where brucellosis is prevalent and highlights this rare neurological complication's distinctive clinical and radiological features. Early recognition and appropriate treatment are crucial to mitigate the significant morbidity associated with neurobrucellosis.
Asunto(s)
Brucelosis , Polirradiculoneuropatía , Humanos , Femenino , Brucelosis/diagnóstico , Brucelosis/complicaciones , Brucelosis/tratamiento farmacológico , Persona de Mediana Edad , Polirradiculoneuropatía/diagnóstico , Polirradiculoneuropatía/microbiología , Antibacterianos/uso terapéutico , Brucella/aislamiento & purificaciónRESUMEN
A panel of 10 IgG enzyme-linked immunosorbent assays (ELISAs) were developed for the detection of anti-microbial immune responses in the cerebrospinal fluid (CSF) of patients with demyelinating diseases (DD). The anti-microbial ELISA assays follow on prior human brain tissue RNA sequencing studies that established multiple sclerosis (MS) microbial candidates. Lysates included in the ELISA panel were derived from Akkermansia muciniphila, Atopobium vaginae, Bacteroides fragilis, Lactobacillus paracasei, Odoribacter splanchnicus, Pseudomonas aeruginosa, Cutibacterium (Propionibacterium) acnes, Fusobacterium necrophorum, Porphyromonas gingivalis, and Streptococcus mutans. CSF responses from patients with demyelinating diseases (DD, N = 14) were compared to those with other neurological diseases (OND, N = 8) and controls (N = 13). Commercial positive and negative control CSF specimens were run with each assay. ELISA index values were derived for each specimen against each of the 10 bacterial lysates. CSF reactivity was significantly higher in the DD group compared to the controls against Akkermansia, Atopobium, Bacteroides, Lactobacillus, Odoribacter, and Fusobacterium. Four of the 11 tested DD group subjects had elevated antibody indexes against at least one of the 10 bacterial species, suggesting intrathecal antibody production. This CSF serological study supports the hypothesis that several of the previously identified MS candidate microbes contribute to demyelination in some patients. KEY MESSAGES: A panel of 10 IgG enzyme-linked immunosorbent assays (ELISAs) were developed for the detection of anti-microbial immune responses in the cerebrospinal fluid (CSF) of patients with demyelinating diseases, including multiple sclerosis and acute disseminated encephalomyelitis. CSF reactivity was significantly higher in the demyelination group compared to the controls against the bacteria Akkermansia, Atopobium, Bacteroides, Lactobacillus, Odoribacter, and Fusobacterium. Several of the demyelination subjects had elevated antibody indexes against at least one of the 10 antigens, suggesting at least limited intrathecal production of anti-bacterial antibodies. This CSF serological study supports the hypothesis that several of the previously identified MS candidate microbes contribute to demyelination in some patients.
Asunto(s)
Anticuerpos Antibacterianos/inmunología , Bacterias/inmunología , Líquido Cefalorraquídeo/inmunología , Inmunoglobulina G/inmunología , Esclerosis Múltiple/inmunología , Polirradiculoneuropatía/inmunología , Adolescente , Adulto , Anciano , Autoanticuerpos/inmunología , Líquido Cefalorraquídeo/microbiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Esclerosis Múltiple/microbiología , Polirradiculoneuropatía/microbiología , Adulto JovenRESUMEN
BACKGROUND: Acute polyradiculoneuritis (APN) is an immune-mediated peripheral nerve disorder in dogs that shares many similarities with Guillain-Barré syndrome (GBS) in humans, in which the bacterial pathogen Campylobacter spp. now is considered to be a major triggering agent. Little information is available concerning the relationship between APN and Campylobacter spp. in dogs. HYPOTHESIS/OBJECTIVES: To estimate the association between Campylobacter spp. infection and APN. Associations with additional potential risk factors also were investigated, particularly consumption of raw chicken. ANIMALS: Twenty-seven client-owned dogs suffering from suspected APN and 47 healthy dogs, client-owned or owned by staff members. METHODS: Case-control study with incidence density-based sampling. Fecal samples were collected from each enrolled animal to perform direct culture, DNA extraction, and polymerase chain reaction (PCR) for detection of Campylobacter spp. In some cases, species identification was performed by sequence analysis of the amplicon. Data were obtained from the medical records and owner questionnaires in both groups. RESULTS: In cases in which the fecal sample was collected within 7 days from onset of clinical signs, APN cases were 9.4 times more likely to be positive for Campylobacter spp compared to control dogs (P < 0.001). In addition, a significant association was detected between dogs affected by APN and the consumption of raw chicken (96% of APN cases; 26% of control dogs). The most common Campylobacter spp. identified was Campylobacter upsaliensis. CONCLUSIONS AND CLINICAL IMPORTANCE: Raw chicken consumption is a risk factor in dogs for the development of APN, which potentially is mediated by infection with Campylobacter spp.
Asunto(s)
Infecciones por Campylobacter/veterinaria , Campylobacter/aislamiento & purificación , Enfermedades de los Perros/microbiología , Polirradiculoneuropatía/veterinaria , Animales , Australia/epidemiología , Campylobacter/genética , Infecciones por Campylobacter/complicaciones , Campylobacter upsaliensis/genética , Campylobacter upsaliensis/aislamiento & purificación , Estudios de Casos y Controles , Pollos , ADN Bacteriano , Dieta/veterinaria , Perros , Heces/microbiología , Reacción en Cadena de la Polimerasa/veterinaria , Polirradiculoneuropatía/complicaciones , Polirradiculoneuropatía/microbiología , Factores de RiesgoAsunto(s)
Neumonía Neumocócica/complicaciones , Polirradiculoneuropatía/etiología , Enfermedad Aguda , Amoxicilina/uso terapéutico , Antibacterianos/uso terapéutico , Bacteriemia/etiología , Bacteriemia/microbiología , Terapia Combinada , Diabetes Mellitus Tipo 2/complicaciones , Susceptibilidad a Enfermedades , Parálisis Facial/etiología , Resultado Fatal , Femenino , Humanos , Inmunoglobulinas Intravenosas/uso terapéutico , Madagascar/etnología , Persona de Mediana Edad , Neumonía Neumocócica/tratamiento farmacológico , Neumonía Neumocócica/terapia , Polirradiculoneuropatía/microbiología , Polirradiculoneuropatía/terapia , Cuadriplejía/etiología , Trastornos Respiratorios/etiología , Trastornos Respiratorios/terapia , Respiración Artificial , FumarRESUMEN
BACKGROUND: Acute canine polyradiculoneuritis (ACP) is considered to be an animal model of the acute axonal form of Guillain-Barré syndrome (GBS) in humans. Various antecedent events have been associated with GBS, including bacterial or viral infection. The relationship between ACP and previous infection requires additional attention. HYPOTHESIS: We hypothesized a relationship between ACP and serological evidence of exposure to Ehrlichia canis, Borrelia burgdorferi, Toxoplasma gondii, Neospora caninum, Campylobacter jejuni, and canine distemper virus (CDV). ANIMALS: Eighty-eight client-owned dogs, 44 with ACP, 44 age-matched controls. METHODS: Retrospective study with stored serum samples. Serum antibodies against the target organisms were measured with commercially available assays. Sera from dogs with and without ACP that were positive for T. gondii IgG by ELISA were assayed by an IgG heavy chain-specific, Western blot immunoassay. RESULTS: Dogs with ACP (55.8%) were more likely to have T. gondii IgG serum antibody titers than dogs without ACP (11.4%). Serum antibodies from 8 affected dogs and 11 control dogs bound to T. gondii antigens with apparent molecular masses of 67, 61, 58, 45, 33, 24, 9, and 6 kDa. An antigen with an apparent molecular mass of 36 kDa was recognized by 2 dogs with ACP but none of the control dogs. CONCLUSIONS: Results of this study suggest that ACP in some dogs, like GBS in some humans, may be triggered by T. gondii and a prospective study should be performed to further evaluate this potential association.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Anticuerpos Antiprotozoarios/sangre , Anticuerpos Antivirales/sangre , Enfermedades de los Perros/epidemiología , Polirradiculoneuropatía/veterinaria , Animales , Western Blotting/veterinaria , Borrelia burgdorferi/inmunología , Campylobacter jejuni/inmunología , Estudios de Casos y Controles , Virus del Moquillo Canino/inmunología , Enfermedades de los Perros/microbiología , Enfermedades de los Perros/parasitología , Enfermedades de los Perros/virología , Perros , Ehrlichia canis/inmunología , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Masculino , Neospora/inmunología , Polirradiculoneuropatía/epidemiología , Polirradiculoneuropatía/microbiología , Polirradiculoneuropatía/parasitología , Estudios Prospectivos , Estudios Seroepidemiológicos , Toxoplasma/inmunologíaRESUMEN
Most reports of autoimmune response during infection with the parasite Trypanosoma cruzi have dealt with the cardiomyopathic form of Chagas' disease, but little is known about the mechanisms of tissue damage involved in the gastrointestinal form, which was studied here. Chronically infected patients with a severe gastrointestinal form of Chagas' disease present increased antibody production and proliferative responses to peripheral myelin components, such as myelin basic protein (MBP), which is homologous to the P1 protein fraction of peripheral myelin. T lymphocytes preferentially recognize a region on the MBP molecule (1-30), which suggests that the MBP is a potential target on the peripheral nerve for autoimmune reactions in patients with gastrointestinal lesions resulting from Chagas' disease.
Asunto(s)
Enfermedad de Chagas/inmunología , Sistema Nervioso Entérico/inmunología , Enfermedades Gastrointestinales/inmunología , Vaina de Mielina/inmunología , Adulto , Animales , Autoanticuerpos/inmunología , Autoantígenos/inmunología , Autoinmunidad/inmunología , Encéfalo/inmunología , Encéfalo/patología , Encéfalo/fisiopatología , Enfermedad de Chagas/fisiopatología , Sistema Nervioso Entérico/patología , Sistema Nervioso Entérico/fisiopatología , Femenino , Enfermedades Gastrointestinales/microbiología , Enfermedades Gastrointestinales/fisiopatología , Tracto Gastrointestinal/inmunología , Tracto Gastrointestinal/inervación , Tracto Gastrointestinal/fisiopatología , Humanos , Activación de Linfocitos/inmunología , Masculino , Persona de Mediana Edad , Proteína Básica de Mielina/inmunología , Proteínas de la Mielina/inmunología , Vaina de Mielina/patología , Neuronas/inmunología , Neuronas/patología , Nervios Periféricos/inmunología , Nervios Periféricos/patología , Nervios Periféricos/fisiopatología , Polirradiculoneuropatía/inmunología , Polirradiculoneuropatía/microbiología , Polirradiculoneuropatía/fisiopatología , Linfocitos T/inmunología , Trypanosoma cruzi/fisiologíaRESUMEN
We report the autopsy findings for a 45-year-old man with polyradiculoneuropathy and fatal acute disseminated encephalomyelitis after having Mycoplasma pneumoniae pneumonia. M. pneumoniae antigens were demonstrated by immunohistochemical analysis of brain tissue, indicating neuroinvasion as an additional pathogenetic mechanism in central neurologic complications of M. pneumoniae infection.
Asunto(s)
Encefalomielitis/microbiología , Mycoplasma pneumoniae/aislamiento & purificación , Neumonía por Mycoplasma/complicaciones , Polirradiculoneuropatía/microbiología , Enfermedad Aguda , Anticuerpos Antibacterianos/sangre , Anticuerpos Antibacterianos/líquido cefalorraquídeo , Antígenos Bacterianos/aislamiento & purificación , Encéfalo/microbiología , Encéfalo/patología , Encefalomielitis/complicaciones , Encefalomielitis/patología , Resultado Fatal , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Mycoplasma pneumoniae/inmunología , Polirradiculoneuropatía/complicaciones , Polirradiculoneuropatía/patologíaRESUMEN
We report a case of partially treated brucellosis that developed quadriparesis, sixth and seventh cranial nerve palsy, and apnea. Electrodiagnostic studies were in favor of acute axonal poly-radiculoneuropathy. Crossreactive immunological responses due to molecular mimicry between Brucella lipooligosaccharide and GM1 ganglioside may justify the development of acute axonal polyradiculoneuropathy after brucellosis.
Asunto(s)
Brucelosis/complicaciones , Polirradiculoneuropatía/etiología , Polirradiculoneuropatía/microbiología , Enfermedad Aguda , Adulto , Apnea/etiología , Apnea/microbiología , Brucelosis/inmunología , Enfermedades de los Nervios Craneales/etiología , Enfermedades de los Nervios Craneales/microbiología , Electrodiagnóstico , Gangliósido G(M1)/inmunología , Humanos , MasculinoRESUMEN
BACKGROUND: Clinical and serologic studies suggest that Guillain-Barré syndrome (GBS) and atypical GBS with preserved muscle stretch reflexes (MSRs) form a continuous spectrum as well as do Fisher syndrome (FS), FS/GBS overlap, Bickerstaff brainstem encephalitis (BBE), BBE/GBS overlap, acute ophthalmoparesis (AO), ataxic GBS, and acute oropharyngeal palsy. OBJECTIVE: To clarify the spectrum of neurologic disorders that occur subsequent to Campylobacter jejuni enteritis. METHODS: We recruited patients with various neurologic conditions and from whom C jejuni was isolated. Bacterial features were investigated. RESULTS: Diagnoses for the patients from whom C jejuni was isolated were GBS (n = 90), FS (n = 22), MSR-preserved GBS (n = 10), FS/GBS (n = 6), BBE (n = 1), BBE/GBS (n = 2), AO (n = 3), ataxic GBS (n = 1), and acute oropharyngeal palsy (n = 3). Isolates from MSR-preserved GBS were similar to those of GBS in serotype (HS:19), genotype (lipo-oligosaccharide [LOS] locus class A or B, cst-II genotype [Thr51]), and GM1 or GD1a epitope expression on LOS. FS/GBS overlap, BBE, BBE/GBS overlap, AO, ataxic GBS, and acute oropharyngeal palsy isolates were similar to those of FS in serotype (HS:2 or HS:4-complex), genotype (LOS locus class A or B, cst-II genotype [Asn51]), and GQ1b epitope expression on LOS. CONCLUSIONS: The bacterial findings support the proposal that Guillain-Barré syndrome (GBS) and muscle stretch reflex-preserved GBS comprise a continuous spectrum as well as do Fisher syndrome (FS), FS/GBS overlap, Bickerstaff brainstem encephalitis (BBE), BBE/GBS overlap, acute ophthalmoparesis, ataxic GBS, and acute oropharyngeal palsy.
Asunto(s)
Infecciones por Campylobacter/complicaciones , Infecciones por Campylobacter/microbiología , Campylobacter jejuni/patogenicidad , Enteritis/complicaciones , Enteritis/microbiología , Polirradiculoneuropatía/microbiología , Adolescente , Adulto , Anciano , Autoanticuerpos/sangre , Autoanticuerpos/genética , Autoanticuerpos/inmunología , Tronco Encefálico/microbiología , Tronco Encefálico/fisiopatología , Infecciones por Campylobacter/inmunología , Campylobacter jejuni/inmunología , Niño , Diagnóstico Diferencial , Encefalitis/inmunología , Encefalitis/microbiología , Encefalitis/fisiopatología , Enteritis/inmunología , Mapeo Epitopo , Femenino , Síndrome de Guillain-Barré/inmunología , Síndrome de Guillain-Barré/microbiología , Síndrome de Guillain-Barré/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Síndrome de Miller Fisher/inmunología , Síndrome de Miller Fisher/microbiología , Síndrome de Miller Fisher/fisiopatología , Polirradiculoneuropatía/inmunología , Polirradiculoneuropatía/fisiopatología , Serotipificación , Especificidad de la EspecieAsunto(s)
Axones/patología , Brucelosis/complicaciones , Polirradiculoneuropatía/etiología , Adulto , Animales , Antibacterianos/uso terapéutico , Brucella/metabolismo , Brucelosis/diagnóstico , Brucelosis/tratamiento farmacológico , Brucelosis/patología , Líquido Cefalorraquídeo/microbiología , Humanos , Masculino , Polirradiculoneuropatía/microbiología , Polirradiculoneuropatía/patologíaAsunto(s)
Inmunoglobulina M/sangre , Enfermedad de la Neurona Motora/microbiología , Mycoplasma pneumoniae , Neumonía por Mycoplasma/complicaciones , Polirradiculoneuropatía/microbiología , Adulto , Axones/inmunología , Axones/patología , Femenino , Humanos , Enfermedad de la Neurona Motora/sangre , Enfermedad de la Neurona Motora/etiología , Neumonía por Mycoplasma/sangre , Polirradiculoneuropatía/inmunologíaRESUMEN
Opsoclonus is characterized by sudden onset of arrhythmic, multidirectional chaotic eye movements, generally associated with a parainfectious or paraneoplasic condition. Opsoclonus is a rare manifestation with no known link with infection by Mycoplasma pneumoniae. We present a case of a 23 year old male patient who presented fever, myalgia, vomiting, respiratory failure, opsoclonus and symmetric flaccid tetraparesis with important lower limb muscle atrophy. Serology and seroreversion for M. pneumoniae were both positive. This case illustrates the possibility that M. pneumoniae may produce a predominantly neurological disease and the need to include this infection in the differential diagnosis of opsoclonus, or polyradiculoneuritis.
Asunto(s)
Encefalitis/complicaciones , Trastornos de la Motilidad Ocular/etiología , Neumonía por Mycoplasma/complicaciones , Polirradiculoneuropatía/complicaciones , Adulto , Encefalitis/microbiología , Humanos , Masculino , Polirradiculoneuropatía/microbiologíaRESUMEN
Several explanations have been proposed to explain the relationship between axonal forms of acute auto-immune inflammatory polyradiculoneuritis and Campylobacter jejuni. The major hypothesis involving molecular imitation is based on the existence of common antigenic determinants (epitopes) in the lipopolysaccharides of the infectious agent and gangliosides, i.e. glycosphingolipides on the surface of the nervous system cells, especially peripheral nervous system cells. The purpose of this literature review is to improve understanding of the rather complex physiopathological mechanisms underlying Guillain-Barre syndrome.
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Enfermedades Autoinmunes/microbiología , Infecciones por Campylobacter/inmunología , Campylobacter jejuni/inmunología , Polirradiculoneuropatía/inmunología , Polirradiculoneuropatía/microbiología , Antígenos Bacterianos/inmunología , Autoantígenos/inmunología , Enfermedades Autoinmunes/inmunología , Epítopos/inmunología , Glicoesfingolípidos/química , Glicoesfingolípidos/inmunología , Humanos , Lipopolisacáridos/química , Lipopolisacáridos/inmunología , Sistema Nervioso Periférico/inmunología , Polirradiculoneuropatía/fisiopatologíaAsunto(s)
Infecciones por Mycoplasma/complicaciones , Mycoplasma pneumoniae , Polirradiculoneuropatía/microbiología , Enfermedad Aguda , Antiinfecciosos/uso terapéutico , Antiinflamatorios/uso terapéutico , Ciprofloxacina/uso terapéutico , Quimioterapia Combinada , Electromiografía , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Infecciones por Mycoplasma/diagnóstico , Infecciones por Mycoplasma/tratamiento farmacológico , Polirradiculoneuropatía/líquido cefalorraquídeo , EsteroidesAsunto(s)
Axones/microbiología , Tronco Encefálico/microbiología , Encefalitis/microbiología , Infecciones por Mycoplasma/complicaciones , Mycoplasma pneumoniae/inmunología , Polirradiculoneuropatía/microbiología , Infecciones del Sistema Respiratorio/complicaciones , Raíces Nerviosas Espinales/microbiología , Adulto , Antibacterianos/uso terapéutico , Axones/inmunología , Axones/patología , Tronco Encefálico/inmunología , Tronco Encefálico/patología , Cauda Equina/inmunología , Cauda Equina/microbiología , Cauda Equina/patología , Encefalitis/inmunología , Encefalitis/fisiopatología , Femenino , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina G/inmunología , Imagen por Resonancia Magnética , Mycoplasma pneumoniae/patogenicidad , Polirradiculoneuropatía/inmunología , Polirradiculoneuropatía/fisiopatología , Cuadriplejía/microbiología , Cuadriplejía/patología , Cuadriplejía/fisiopatología , Recuperación de la Función/inmunología , Raíces Nerviosas Espinales/inmunología , Raíces Nerviosas Espinales/patologíaRESUMEN
Guillain-Barré syndrome and its variant, Miller-Fisher syndrome, are acute, postinfectious, autoimmune neuropathies that frequently follow Campylobacter jejuni enteritis. The pathogenesis is believed to involve molecular mimicry between sialylated epitopes on C. jejuni LPSs and neural gangliosides. More than 90% of Miller-Fisher syndrome cases have serum anti-GQ1b and anti-GT1a ganglioside antibodies that may also react with other disialylated gangliosides including GD3 and GD1b. Structural studies on LPS from neuropathy-associated C. jejuni strains have revealed GT1a-like and GD3-like core oligosaccharides. To determine whether this structural mimicry results in pathogenic autoantibodies, we immunized mice with GT1a/GD3-like C. jejuni LPS and then cloned mAb's that reacted with both the immunizing LPS and GQ1b/GT1a/GD3 gangliosides. Immunohistology demonstrated antibody binding to ganglioside-rich sites including motor nerve terminals. In ex vivo electrophysiological studies of nerve terminal function, application of antibodies either ex vivo or in vivo via passive immunization induced massive quantal release of acetylcholine, followed by neurotransmission block. This effect was complement-dependent and associated with extensive deposits of IgM and C3c at nerve terminals. These data provide strong support for the molecular mimicry hypothesis as a mechanism for the induction of cross-reactive pathogenic anti-ganglioside/LPS antibodies in postinfectious neuropathies.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Campylobacter jejuni/inmunología , Gangliósidos/inmunología , Lipopolisacáridos/inmunología , Unión Neuromuscular/fisiología , Polirradiculoneuropatía/microbiología , Animales , Complemento C3/fisiología , Reacciones Cruzadas , Femenino , Inmunización , Inmunoglobulina M/inmunología , Masculino , Ratones , Ratones Endogámicos , Nervios Periféricos/inmunologíaRESUMEN
We examined the reactivity of a panel of anti-GM1 immunoglobulin M monoclonal antibodies (MAbs) cloned from multifocal motor neuropathy patients with lipopolysaccharides (LPSs) of Campylobacter jejuni strains, including serotype O:41 strains associated with Guillain-Barré syndrome. The MAbs reacted with ganglioside GM1 to different degrees, and these differences in fine specificities for GM1 were reflected in the different degrees of reactivity with each of the C. jejuni LPSs tested. Antibodies could also be discriminated by the varying patterns of inhibition by cholera toxin (a GM1 ligand) in LPS binding studies. These results indicate that there is a substantial heterogeneity among C. jejuni O:41 strains in their expression of GM1-like epitopes and among the fine specificities of different neuropathy-associated anti-GM1 antibodies.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Campylobacter jejuni/inmunología , Gangliósido G(M1)/inmunología , Inmunoglobulina M/inmunología , Lipopolisacáridos/inmunología , Polirradiculoneuropatía/inmunología , Anticuerpos Antibacterianos/inmunología , Especificidad de Anticuerpos , Reacciones Cruzadas , Humanos , Polirradiculoneuropatía/microbiologíaRESUMEN
Campylobacter curvus and Campylobacter upsaliensis were isolated from stools of patients with Guillain-Barré (GBS) or Fisher's (FS) syndromes. Whether these microorganisms are pathogens of antecedent diarrhea in GBS and FS is not clear, therefore, we made a serological examination. There were no differences in antibody titer to these organisms among the patients with GBS, FS, and the controls. Some patients had elevated antibodies to the bacteria, but most also had serological evidence of C. jejuni infection. Moreover, the patients from whom C. curvus had been isolated did not have antibodies to the bacterium, indicative that they were healthy carriers of C. curvus or that the isolates were the product of contamination. We conclude that neither C. curvus nor C. upsaliensis is the major agent of antecedent diarrhea in GBS and FS.