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1.
Eur J Med Chem ; 141: 169-177, 2017 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-29031064

RESUMEN

Indoleamine 2,3-dioxygenase 1 (IDO1) is attracting a great deal of interest as drug target in immune-oncology being highly expressed in cancer cells and participating to the tumor immune-editing process. Although several classes of IDO1 inhibitors have been reported in literature and patent applications, only few compounds have proved optimal pharmacological profile in preclinical studies to be advanced in clinical trials. Accordingly, the quest for novel structural classes of IDO1 inhibitors is still open. In this paper, we report a fragment-based screening campaign that combines Water-LOGSY NMR experiments and microscale thermophoresis approach to identify fragments that may be helpful for the development of novel IDO1 inhibitors as therapeutic agents in immune-oncology disorders.


Asunto(s)
Inhibidores Enzimáticos/farmacología , Indolamina-Pirrol 2,3,-Dioxigenasa/antagonistas & inhibidores , Quinurenina/farmacología , Línea Celular , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Inhibidores Enzimáticos/síntesis química , Inhibidores Enzimáticos/química , Humanos , Indolamina-Pirrol 2,3,-Dioxigenasa/metabolismo , Quinurenina/síntesis química , Quinurenina/química , Simulación del Acoplamiento Molecular , Estructura Molecular , Relación Estructura-Actividad
2.
Sci Rep ; 3: 1560, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23532089

RESUMEN

Age-onset cataracts are believed to be expedited by the accumulation of UV-damaged human γD-crystallins in the eye lens. Here we show with molecular dynamics simulations that the stability of γD-crystallin is greatly reduced by the conversion of tryptophan to kynurenine due to UV-radiation, consistent with previous experimental evidences. Furthermore, our atomic-detailed results reveal that kynurenine attracts more waters and other polar sidechains due to its additional amino and carbonyl groups on the damaged tryptophan sidechain, thus breaching the integrity of nearby dry center regions formed by the two Greek key motifs in each domain. The damaged tryptophan residues cause large fluctuations in the Tyr-Trp-Tyr sandwich-like hydrophobic clusters, which in turn break crucial hydrogen-bonds bridging two ß-strands in the Greek key motifs at the "tyrosine corner". Our findings may provide new insights for understanding of the molecular mechanism of the initial stages of UV-induced cataractogenesis.


Asunto(s)
Estabilidad Proteica/efectos de la radiación , Desplegamiento Proteico/efectos de la radiación , Triptófano/efectos de la radiación , Rayos Ultravioleta , gamma-Cristalinas/efectos de la radiación , Catarata/etiología , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Quinurenina/síntesis química , Quinurenina/química , Quinurenina/genética , Simulación de Dinámica Molecular , Triptófano/química , Tirosina/efectos de la radiación , gamma-Cristalinas/química
3.
Bioorg Med Chem Lett ; 22(8): 2734-7, 2012 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-22444682

RESUMEN

We have prepared l-kyurenine 4-hydroxyphenacyl ester, a caged derivative of L-kynurenine. N(α)-tBOC-L-tryptophan was reacted with 4-hydroxyphenacyl bromide in DMF with K(2)CO(3) as the base to give the N(α)-tBOC 4-hydroxyphenacyl ester. The ester was then treated with O(3) in MeOH at -20°C, followed by trifluoroacetic acid in CH(2)Cl(2), then aqueous HCl to obtain the caged kynurenine as the dihydrochloride salt. The caged kynurenine is stable as a dry solid in the dark at -78°C, but in aqueous solutions in phosphate buffer at pH 7-8 hydrolyzes rapidly (t(1/2) ∼5 min). Solutions in Tris at pH 7 are more stable (t(1/2) >30 min), and solutions in 1mM HCl are stable for several hours. As expected, the ester is cleaved in microseconds with laser pulses at 355 nm. The caged kynurenine may be useful for preparation of substrate complexes for crystallography or in biological studies on kynurenine.


Asunto(s)
Quinurenina/análogos & derivados , Quinurenina/química , Quinurenina/síntesis química , Rayos Láser , Acetofenonas/química , Estructura Molecular
4.
Biochemistry ; 50(25): 5680-92, 2011 Jun 28.
Artículo en Inglés | MEDLINE | ID: mdl-21612226

RESUMEN

The antitumor antibiotic sibiromycin belongs to the class of pyrrolo[1,4]benzodiazepines (PBDs) that are produced by a variety of actinomycetes. PBDs are sequence-specific DNA-alkylating agents and possess significant antitumor properties. Among them, sibiromycin, one of two identified glycosylated PBDs, displays the highest DNA binding affinity and the most potent antitumor activity. In this study, we report the elucidation of the precise reaction sequence leading to the formation and activation of the 3,5-dihydroxy-4-methylanthranilic acid building block found in sibiromycin, starting from the known metabolite 3-hydroxykynurenine (3HK). The investigated pathway consists of four enzymes, which were biochemically characterized in vitro. Starting from 3HK, the SAM-dependent methyltransferase SibL converts the substrate to its 4-methyl derivative, followed by hydrolysis through the action of the PLP-dependent kynureninase SibQ, leading to 3-hydroxy-4-methylanthranilic acid (3H4MAA) formation. Subsequently the NRPS didomain SibE activates 3H4MAA and tethers it to its thiolation domain, where it is hydroxylated at the C5 position by the FAD/NADH-dependent hydroxylase SibG yielding the fully substituted anthranilate moiety found in sibiromycin. These insights about sibiromycin biosynthesis and the substrate specificities of the biosynthetic enzymes involved may guide future attempts to engineer the PBD biosynthetic machinery and help in the production of PBD derivatives.


Asunto(s)
Aminoglicósidos/síntesis química , Antibióticos Antineoplásicos/síntesis química , Benzodiazepinas/síntesis química , Pirroles/síntesis química , ortoaminobenzoatos/síntesis química , Actinomycetales/enzimología , Aminoglicósidos/metabolismo , Antibióticos Antineoplásicos/metabolismo , Benzodiazepinas/metabolismo , Glicosilación , Hidrolasas/química , Hidrolasas/metabolismo , Quinurenina/análogos & derivados , Quinurenina/síntesis química , Quinurenina/metabolismo , Metiltransferasas/química , Metiltransferasas/metabolismo , Pirofosfatasas/química , Pirofosfatasas/metabolismo , Pirroles/metabolismo , Estereoisomerismo , Especificidad por Sustrato , ortoaminobenzoatos/metabolismo
5.
Invest Ophthalmol Vis Sci ; 48(8): 3705-13, 2007 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-17652742

RESUMEN

PURPOSE: Human ultraviolet light (UV) filters, such as kynurenine (Kyn), readily deaminate to reactive unsaturated ketones that covalently modify proteins in older human lenses. The aim of this study was to examine in vitro rates of formation and decomposition of the three major Kyn-amino acid adducts and possible consequences for the lens. METHODS: The t-Boc-protected Kyn-His, Kyn-Lys, and Kyn-Cys adducts and Kyn-Cys were synthesized from the corresponding amino acids and Kyn. Calf lens proteins were modified with Kyn by incubation at pH 7. Stability and competition studies of the adducts were conducted under physiological conditions. Kyn-amino acids and their decomposition products were quantified using HPLC. RESULTS: At physiological pH, Kyn-Cys adducts formed more rapidly than either Lys or His adducts, but they also decomposed readily. By contrast, His adducts were stable. Cysteine (Cys) residues in beta-crystallins were major sites of modification. The Kyn moiety, initially bound to Cys residues, was found to transfer to other amino acids. Glutathione promoted the breakdown of Kyn-Cys. CONCLUSIONS: These data may help explain why proteins in young lenses are not modified by UV filters in situ. The initial phase of the modification of proteins in the human lens by UV filters may be a dynamic process. In lenses, Cys residues of crystallins modify preferentially, but these adducts also decompose to release deaminated Kyn. This can then potentially react with other amino acids. Glutathione, which is present in high concentrations in the lenses of young people, may play a vital role in keeping proteins free from modification by intercepting reactive deaminated kynurenines formed by the spontaneous breakdown of free UV filters, promoting the decomposition of Kyn-Cys residues, and sequestering the unsaturated ketones once they are released from modified proteins.


Asunto(s)
Envejecimiento/metabolismo , Cristalinas/metabolismo , Glutatión/metabolismo , Quinurenina/farmacocinética , Cristalino/metabolismo , Animales , Unión Competitiva/fisiología , Bovinos , Cisteína/síntesis química , Cisteína/farmacocinética , Glutatión/química , Histidina/síntesis química , Histidina/farmacocinética , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Cetonas/metabolismo , Quinurenina/síntesis química , Cristalino/efectos de la radiación , Procesamiento Proteico-Postraduccional/fisiología , Rayos Ultravioleta
6.
BMC Biochem ; 4: 13, 2003 Sep 24.
Artículo en Inglés | MEDLINE | ID: mdl-14505498

RESUMEN

BACKGROUND: Kynureninase is a key enzyme on the kynurenine pathway of tryptophan metabolism. One of the end products of the pathway is the neurotoxin quinolinic acid which appears to be responsible for neuronal cell death in a number of important neurological diseases. This makes kynureninase a possible therapeutic target for diseases such as Huntington's, Alzheimer's and AIDS related dementia, and the development of potent inhibitors an important research aim. RESULTS: Two new kynurenine analogues, 3-hydroxydesaminokynurenine and 3-methoxydesaminokynurenine, were synthesised as inhibitors of kynureninase and tested on the tryptophan-induced bacterial enzyme from Pseudomonas fluorescens, the recombinant human enzyme and the rat hepatic enzyme. They were found to be mixed inhibitors of all three enzymes displaying both competitive and non competitive inhibition. The 3-hydroxy derivative gave low Ki values of 5, 40 and 100 nM respectively. An improved 3-step purification scheme for recombinant human kynureninase was also developed. CONCLUSION: For kynureninase from all three species the 2-amino group was found to be crucial for activity whilst the 3-hydroxyl group played a fundamental role in binding at the active site presumably via hydrogen bonding. The potency of the various inhibitors was found to be species specific. The 3-hydroxylated inhibitor had a greater affinity for the human enzyme, consistent with its specificity for 3-hydroxykynurenine as substrate, whilst the methoxylated version yielded no significant difference between bacterial and human kynureninase. The modified purification described is relatively quick, simple and cost effective.


Asunto(s)
Inhibidores Enzimáticos/farmacología , Hidrolasas/metabolismo , Quinurenina/farmacología , Animales , Cromatografía por Intercambio Iónico , Electroforesis en Gel de Poliacrilamida , Inhibidores Enzimáticos/síntesis química , Inhibidores Enzimáticos/química , Humanos , Hidrolasas/genética , Quinurenina/análogos & derivados , Quinurenina/síntesis química , Quinurenina/química , Ratas , Proteínas Recombinantes/aislamiento & purificación
7.
J Agric Food Chem ; 50(15): 4303-9, 2002 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-12105962

RESUMEN

Kynurenine (1) and indole-3-acetic acid (2) are considered as potential precursors of 2-aminoacetophenone (3), which is regarded to be the aroma impact compound causing an "untypical aging off-flavor" (UTA) in Vitis vinifera wines. The mechanism of the formation of 3 was studied using model fermentation and model sulfuration media spiked with 1 or 2 as potential precursors. Possible degradation products such as kynurenamine (4) and kynurenic acid (5), or skatole (6), 2-oxoskatole (7), 2-formamidoacetophenone (8), 2-oxindole-3-acetic acid (9), and 3-(2-formylaminophenyl)-3-oxopropionic acid (10) were evaluated by HPLC-UV of the fermentation and sulfuration media and comparison with synthesized 7, 8, 9, and 10. The synthesis of the possible precursor 4-(2-aminophenyl)-2,4-dioxobutanoic acid (11), a proposed metabolite of 1 failed because a spontaneous cyclization yields 5 and N-oxo-kynurenic acid (12), but not 11. It could be shown that the formation of 3 is triggered by an oxidative degradation of 2 after sulfuration with potassium bisulfite via the intermediates 10 and 8. However, no formation of 3 occurred during sulfuration of a model wine spiked with 1 or during fermentation of a model must spiked with 1 or 2.


Asunto(s)
Acetofenonas/análisis , Tecnología de Alimentos , Ácidos Indolacéticos/síntesis química , Quinurenina/síntesis química , Odorantes , Vino/análisis , Acetofenonas/química , Cromatografía Líquida de Alta Presión , Fermentación , Ácidos Indolacéticos/metabolismo , Quinurenina/metabolismo , Saccharomyces cerevisiae/metabolismo , Azufre/química , Gusto , Factores de Tiempo
8.
J Med Chem ; 45(2): 263-74, 2002 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-11784131

RESUMEN

The overstimulation of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors is involved in excitotoxicity, a process participating in neurodegeneration that characterizes some neurological disorders and acute cerebral insults. In looking for compounds with neuroprotective properties, a series of kynurenine derivatives were synthesized, and their effects on both the NMDA and nNOS activity in rat striatum were evaluated. Two compounds, 15a (2-acetamido-4-(2-amino-5-methoxyphenyl)-4-oxobutyric acid) and 15c (2-butyramido-4-(2-amino-5-methoxyphenyl)-4-oxobutyric acid), displayed more potent activities than the other synthetic compounds tested for the inhibition of NMDA excitability and nNOS activity. Two other compounds, 18a (2-acetamido-4-(3-methoxyphenyl)-4-oxobutyric acid) and 18c (2-butyramido-4-(3-methoxyphenyl)-4-oxobutyric acid), that have the same structure as 15a and 15c, except the amino group in R(1), showed different effects. Whereas compound 18a showed lower electrophysiological potency than compounds 15a and 15c in the inhibition of the NMDA-dependent excitability, compound 18c showed the opposite effect. Moreover, compounds 18a and 18c were unable to modify nNOS activity. The remaining kynurenines tested behave like compound 18a. These results suggest that a structure-related activity of these synthetic kynurenines and a N-H bond in a specific direction is necessary for some kynurenine analogues to inhibit nNOS activity.


Asunto(s)
Cuerpo Estriado/efectos de los fármacos , Inhibidores Enzimáticos/síntesis química , Quinurenina/análogos & derivados , Quinurenina/síntesis química , Óxido Nítrico Sintasa/antagonistas & inhibidores , Animales , Cuerpo Estriado/enzimología , Cuerpo Estriado/fisiología , Electrofisiología , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/farmacología , Técnicas In Vitro , Quinurenina/química , Quinurenina/farmacología , Espectroscopía de Resonancia Magnética , Masculino , Modelos Moleculares , Conformación Molecular , Óxido Nítrico Sintasa de Tipo I , Ratas , Ratas Wistar , Relación Estructura-Actividad
9.
Bioorg Med Chem ; 7(8): 1497-503, 1999 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10482441

RESUMEN

The diastereomers of beta-methyl-L-kynurenine were prepared by preparative ozonolysis of the respective diastereomers of beta-methyl-L-tryptophan. A practical method for preparative enzymatic resolution of the diastereomers of beta-methyltryptophan was developed using carboxypeptidase A digestion of the N-trifluoroacetyl derivatives. The stereochemical assignment was confirmed by X-ray crystal structure determination of (2S, 3R)-threo-beta-methyl-L-tryptophan. (2S,3S)-erythro-beta-Methyl-L-kynurenine is a slow substrate for kynureninase from Pseudomonas fluorescens (k(cat)/K(m) = 0.1% that of L-kynurenine), producing anthranilic acid, while (2S,3R)-threo-L-kynurenine is about 390-fold less reactive than erythro. Rapid-scanning stopped-flow measurements show that beta-methyl substitution affects the rate of alpha-deprotonation of the L-kynurenine-pyridoxal-5'-phosphate Schiffs base. This is consistent with the stereoelectronic requirements of the reaction. These results are the first demonstration that beta-substituted kynurenines can be substrates for kynureninase, and may be useful in the design of mechanism-based inhibitors.


Asunto(s)
Hidrolasas/metabolismo , Quinurenina/análogos & derivados , Pseudomonas fluorescens/enzimología , Cinética , Quinurenina/síntesis química , Quinurenina/química , Quinurenina/metabolismo , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Estructura Molecular , Estereoisomerismo , Especificidad por Sustrato
10.
Chem Res Toxicol ; 7(2): 185-90, 1994.
Artículo en Inglés | MEDLINE | ID: mdl-8199307

RESUMEN

The oxygenation of tryptophan and its peptides by the superoxide-generating system hypoxanthine/xanthine oxidase in the presence of iron(III) and ethylenediaminetetraacetic acid (EDTA) has been investigated. The reaction of a tryptophan derivative, N-(tert-butoxycarbonyl)-L-tryptophan, with hypoxanthine/xanthine oxidase/Fe(III)-EDTA mainly resulted in the oxygenation of the pyrrole ring of the indole nucleus. 2-[(tert-Butoxycarbonyl)-amino]-3-(3-oxindolyl)propionic acid and N-(tert-butoxycarbonyl)-N'-formylkynurenine were identified as the major products. Similar oxindole- and formylkynurenine-type products were also obtained from the N-(tert-butoxycarbonyl) derivative of the tryptophan-containing peptides Ile-Trp, Trp-Leu, Gly-Trp-Leu, and Ala-Trp-Ile. In all cases, however, hydroxylation products of the benzene ring of the indole nucleus were scarcely detected, leading to the assumption that free hydroxyl radical did not play a role in the tryptophan oxidation of this system. Of interest was the fact that the reaction of N-(tert-butoxycarbonyl)-L-tryptophan with H2O2/horseradish peroxidase mainly afforded the same oxindole- and formylkynurenine-type products as those obtained in the hypoxanthine/xanthine oxidase/Fe(III)-EDTA system. Taken together, iron-oxygen complex-type active species may play a role in the tryptophan oxygenation in a superoxide-generating system in the presence of iron-EDTA.


Asunto(s)
Ácido Edético/química , Compuestos Férricos/química , Indoles/síntesis química , Quinurenina/análogos & derivados , Oligopéptidos/química , Triptófano/química , Secuencia de Aminoácidos , Quelantes del Hierro/química , Quinurenina/síntesis química , Datos de Secuencia Molecular , Oxidación-Reducción , Oxígeno/química , Superóxidos/química
11.
J Biochem ; 92(1): 129-41, 1982 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-7118867

RESUMEN

Several kynurenine derivatives including N'-formylkynurenine were prepared in high purity by the ozonization of the corresponding indole compounds. The fluorescence characteristics of those derivatives were examined in connection with the use of their fluorophores as reporters for the local environment of tryptophan in proteins. Kynurenine is a weak emitter of fluorescence, with an emission maximum at 480 nm on excitation at 365 nm. With decreasing solvent polarity, the fluorescence intensity increases logarithmically and the emission maximum shifts to blue. A linear relation between these fluorescence characteristics and solvent polarity exists when the polarity is shown in terms of dielectric constant. N'-Formylkynurenine is a somewhat stronger emitter of fluorescence than kynurenine. The emission maximum is 434 nm on excitation at 325 nm and it shifts to blue in solvents of low polarity. This blue shift is also linear with respect to the dielectric constant of the solvent. Other factors influencing kynurenine fluorescence and N'-formylkynurenine fluorescence examined were neighboring groups, ionic strength, temperature, and protein denaturants. Based on the results of the present investigation, the local environment of tryptophan 62 in hen egg-white lysozyme was examined using Kyn 62-lysozyme.


Asunto(s)
Quinurenina/análogos & derivados , Quinurenina/farmacología , Muramidasa/metabolismo , Triptófano , Animales , Sitios de Unión , Pollos , Clara de Huevo , Cinética , Quinurenina/síntesis química , Unión Proteica , Espectrometría de Fluorescencia , Espectrofotometría Ultravioleta
14.
C R Acad Hebd Seances Acad Sci D ; 280(11): 1425-8, 1975 Mar 17.
Artículo en Francés | MEDLINE | ID: mdl-811372

RESUMEN

Evidence for the involvement of O-2 ions in the formation of N'-Formylkynurenine, a major tryptophan oxidation product is presented on the basis of radiolysis and electrochemistry data. The biological importance of such a reaction is discussed.


Asunto(s)
Quinurenina/análogos & derivados , Oxígeno , Triptófano , Aniones , Electroquímica , Quinurenina/síntesis química , Radioquímica
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