RESUMEN
The reticulin-M forte (R forte), an antianaphylactic peptide, extracted from organs rich in RES, previously stimulated with India ink, was analysed after acetone precipitation by paper high voltage electrophoresis. Finally the biological activity remains concentrated in the second, arbitrary group of basic peptides, fractions 1 and 3.
Asunto(s)
Reticulina/análisis , Acetona , Anafilaxia , Animales , Precipitación Química , Electroforesis en Papel/métodos , Femenino , Cobayas , Indicadores y Reactivos , Reticulina/aislamiento & purificación , Reticulina/farmacología , Contracción Uterina/efectos de los fármacosRESUMEN
I. Moldovan (1923) demonstrated in the blood ultrafiltrate from anaphylactically prepared guinea pigs, which believed to anaphylactic shock, or which were previously injected with coloidal substances as India ink to stimulate the RES, an antianaphylactic principle named Reticulin-M(R). Authors extracted R from organs rich in RES with acetone, established their, peptidic nature and tested it on anaphylactic prepared guinea pig uterine horns in vitro. The peptides were fractioned by high voltage paper electrophoresis. The fractions were cut up in four groups, eluted and tested. The second basic group was identified as active and concentrated by acetone, recipitation. In conclusion several techniques were used to obtain, to test, to isolate and to concentrate R, a natural antianaphylactic peptidic factor, which may be a cytokine.
Asunto(s)
Anafilaxia/prevención & control , Sistema Mononuclear Fagocítico/metabolismo , Reticulina/fisiología , Anafilaxia/fisiopatología , Animales , Bovinos , Electroforesis en Papel , Femenino , Cobayas , Músculo Liso/efectos de los fármacos , Reticulina/aislamiento & purificación , Reticulina/farmacología , Contracción Uterina/efectos de los fármacos , Útero/efectos de los fármacosRESUMEN
The collagen in localized and systemic scleroderma skin was studied by light microscopy with silver impregnation (50 patients), electron microscopy (14 patients), and immunofluorescence microscopy using specific antibodies against Type I and Type III collagens (12 patients). In the cellular stage, the dermis and adipose tissue revealed perivascular or diffuse cellular infiltrates (mostly lymphocytes, plasma cells, and macrophages), accompanied by deposition of Type III collagen. The lower dermis also showed an increase in Type III collagen. In the fibrotic stage, the papillary layer showed a reduction and/or clumping of Type III collagen as compared to normal skin. The lower dermis and the adipose tissue revealed compact collagen consisting exclusively of Type I collagen or a mixture of Type I and Type III collagen. The pattern of Type III collagen distribution was similar to that of reticulin, thus suggesting that at least some reticulin fibrils may represent Type III collagen.
