RESUMEN
A novel pale orange-coloured bacterium, designated strain SYSU D00532T, was isolated from sandy soil collected from the Gurbantunggut desert in Xinjiang, PR China. Cells of strain SYSU D00532T were found to be aerobic, Gram-stain-negative, oxidase-positive, catalase-positive, motile and rod-shaped with a single polar or subpolar flagellum. Growth occurred at 15-45 °C (optimum, 28-37 °C, pH 5.0-8.0 (optimum, pH 6.0-7.0) and with 0-1.5% NaCl (w/v; optimum, 0.5â%). The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. Unidentified aminolipids, unidentified polar lipids, an unidentified aminophospholipid and an unidentified phospholipid were also detected. The major respiratory quinone was ubiquinone-10 and the major fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0 and C19:0 cyclo ω8c. The genomic DNA G+C content was 69.8 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU D00532T belonged to the family Azospirillaceae and showed 93.4% (Desertibacter roseus 2622T), 93.2% (Skermanella xinjiangensis 10-1-101T), 93.2% ('Skermanella rubra' YIM 93097T) and 92.4% (Desertibacter xinjiangensis M71T) similarities. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain SYSU D00532T is proposed to represent a new species of a new genus, named Arenibaculum pallidiluteum gen. nov., sp. nov., within the family Azospirillaceae. The type strain is SYSU D00532T (=KCTC 82269T=CGMCC 1.18631T=MCCC 1K04984T). We also propose the reclassification of Skermanella xinjiangensis to a new genus Deserticella as Deserticella xinjiangensis comb. nov., and the transfer of the genera Indioceanicola and Oleisolibacter from the family Rhodospirillaceae to the family Azospirillaceaewe based on the phylogenetic results.
Asunto(s)
Filogenia , Rhodospirillaceae/clasificación , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , China , ADN Bacteriano/genética , Clima Desértico , Pigmentación , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/análogos & derivados , Ubiquinona/químicaRESUMEN
A novel Gram-staining-negative, spiral-shaped bacterium, designated strain 64-1T, was isolated from oil reservoir water collected from Liaohe oilfield, north-eastern China. Growth occurred at 15-55 °C and pH 6.0-10.0. The sole respiratory quinone was Q-10. The predominant cellular fatty acids were summed feature 8 (C18â:â1 ω7c /C18â:â1 ω6c), C16â:â0 and C19â:â0 cyclo ω8c. The polar lipids consisted of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), an unidentified aminophospholipid (UAPL), an unidentified aminolipid (UAL) and two unidentified polar lipids (UPL). The genomic DNA G+C content of strain 64-1T was 64.5 mol%. Strain 64-1T shared the highest 16S rRNA gene sequence similarities with Phaeospirillum chandramohanii JA145T (92.0â%) and Telmatospirillum siberiense 26-4b1T (91.8â%). In the phylogenetic trees, the strain constituted a sub-cluster within the family Rhodospirillaceae. Based on the results of morphological, physiological, biochemical and phylogenetic analysis, strain 64-1T represents a new species of a novel genus within the family Rhodospirillaceae, for which the name Oleiliquidispirillum nitrogeniifigens gen. nov., sp. nov. is proposed. The type strain is 64-1T (=CGMCC 1.16798T=LMG 31399T).
Asunto(s)
Yacimiento de Petróleo y Gas/microbiología , Filogenia , Rhodospirillaceae/clasificación , Microbiología del Agua , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/análogos & derivados , Ubiquinona/químicaRESUMEN
A Gram-stain-negative, non-spore-forming, aerobic, motile, curved rod-shaped bacterium, designed strain R148T was isolated from a coralline algae Tricleocarpa sp. collected from Weizhou island, PR China. The optimal growth of R148T occurred at 25 °C, pH 8-9 in the presence of 0.5â% (w/v) NaCl on the basis of amended marine broth 2216. The genomic DNA G+C content was 59.5 mol%. The only detected respiratory quinone was Q-10. The major polar lipids were phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and three unidentified ninhydrin-positive lipids. The major cellular fatty acids were C18â:â1ω7c, C16â:â1ω7c, C19â:â0cyclo 9, 10 DMA and C18â:â0. The results of 16S rRNA gene-based global alignment indicated that the closest neighbour of strain R148T was Pelagibius litoralis DSM 21314T (93.1â% similarity), the second is Limibacillus halophilus KCTC 42420T (92.2â%). The results of phylogenetic analysis indicated that R148T forms a distinct branch in the robust clade of R148T and P. litoralis DSM 21314T, while the taxonomic position of this clade in the family Rhodospirillaceae is ambiguous among phylogenetic approaches. The low 16S rRNA gene similarity and distinct polar lipid and cellular fatty acid profile could readily distinguish R148T from closely related type strains. So R148T is suggested to represent a novel species in a novel genus, for which the name Denitrobaculum tricleocarpae gen. nov., sp. nov. is proposed. The type strain is R148T (=MCCC 1K03781T=KCTC 72137T).
Asunto(s)
Filogenia , Rhodophyta/microbiología , Rhodospirillaceae/clasificación , Ubiquinona/análogos & derivados , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Islas , Fosfolípidos/química , ARN Ribosómico 16S/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
Autism spectrum disorder (ASD) is a complex of neurodevelopmental conditions with increasing incidence. The microbiota of children with ASD is distinct from neurotypical children, their food habits are also different, and it is known that nutrient intake influences microbiota in a specific way. Thus, this study investigates the food habits of children with ASD and their association with the gut microbiota. Children with ASD had their dietary energy intakes similar to controls, but they more often demonstrated food selectivity, which seemed to result in deficiency of micronutrients such as vitamins K, B6, C, iron, cooper, docosahexaenoic and docosapentanoic acid. Using high-throughput sequencing, a DNA library of intestinal microbiota was performed. Core microbiota was similar in children with and without ASD, but Dichelobacter, Nitriliruptor and Constrictibacter were found to be putative markers of ASD. The changes in gut microbiota that we observed in connection to food selectivity, intake of fats and omega-3 in particular, fermented milk products and animal/plant protein consumption had similar character, independent of diagnosis. However, high fibre intake was connected with a decreased α-diversity only in children with ASD. High carbohydrate and fibre intake influenced ß-diversity, changing the abundance of Bacteroides and other genera, many of them members of the Clostidiaceae. Modulating food habits of ASD children can influence their gut microbiota composition.
Asunto(s)
Trastorno del Espectro Autista/microbiología , Bacterias/clasificación , Análisis de los Alimentos/métodos , Análisis de Secuencia de ADN/métodos , Actinobacteria/aislamiento & purificación , Bacterias/genética , Bacterias/aislamiento & purificación , Estudios de Casos y Controles , Niño , Preescolar , ADN Bacteriano , Dichelobacter nodosus/aislamiento & purificación , Ingestión de Alimentos , Microbioma Gastrointestinal , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Masculino , Rhodospirillaceae/aislamiento & purificaciónRESUMEN
Two strains of the family Rhodospirillaceae were isolated from the rhizosphere of the medicinal plant Hypericum perforatum. Cells of both strains were Gram-stain-negative, motile by means of a single polar flagellum, non-spore-forming, non-capsulated, short rods that divided by binary fission. Colonies were small and white. Strains R5913T and R5959T were oxidase-positive, mesophilic, neutrophilic and grew optimally without NaCl. Both grew under aerobic and microaerophilic conditions and on a limited range of substrates with best results on yeast extract. Major fatty acids were C19â:â0 cyclo ω8c and C16â:â0; in addition, C18â:â1ω7c was also found as a predominant fatty acid in strain R5913T. The major respiratory quinone was ubiquinone 10 (Q-10). The DNA G+C contents of strains R5913T and R5959T were 66.0 and 67.4 mol%, respectively. 16S rRNA gene sequence comparison revealed that the closest relatives (<92â% similarity) of the strains are Oceanibaculum pacificum MCCC 1A02656T, Dongia mobilis CGMCC 1.7660T, Dongia soli D78T and Dongia rigui 04SU4-PT. The two novel strains shared 98.6â% sequence similarity and represent different species on the basis of low average nucleotide identity of their genomes (83.8â%). Based on the combined phenotypic, genomic and phylogenetic investigations, the two strains represent two novel species of a new genus in the family Rhodospirillaceae, for which the name Hypericibacter gen. nov. is proposed, comprising the type species Hypericibacter terrae sp. nov. (type strain R5913T=DSM 109816T=CECT 9472T) and Hypericibacter adhaerens sp. nov. (type strain R5959T=DSM 109817T=CECT 9620T).
Asunto(s)
Hypericum/microbiología , Filogenia , Rizosfera , Rhodospirillaceae/clasificación , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Alemania , ARN Ribosómico 16S/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/análogos & derivados , Ubiquinona/químicaRESUMEN
A novel Gram-stain-negative, light pink-coloured, short rod-shaped, designated strain W17T, was isolated from a meadow soil sample collected from Xinjiang, PR China. The 16S rRNA gene sequence analysis indicated that strain W17T was related most closely to Skermanella rosea M1T (98.72 %) and Skermanella mucosa 8-14-6T (98.44 %). However, strain W17T showed a low level of DNA-DNA relatedness to S. rosea M1T (32.4±2.6 %) and S. mucosa 8-14-6T (33.5±0.1â%). The genome size of the novel strain was 5.87 Mb and the genomic DNA G+C content was 67.27 mol%. The only respiratory quinone of strain W17T was Q-10. Diphosphatidylglycerol, phosphatidylglycerol. phosphatidylethanolamine and phosphatidylcholine were the major polar lipids. The predominant cellular fatty acids were C18â:â1ω6c and/or C18â:â1ω7c (48.53 %), C16â:â0 (20.88 %) and C18â:â0 (14.92 %). The phylogenetic, phenotypic and chemotaxonomic data showed that strain W17T represents a novel species of the genus Skermanella, for which the name Skermanella pratensis sp. nov. is proposed. The type strain is W17T (=GDMCC 1.1392T=KCTC 62434T).
Asunto(s)
Filogenia , Rhodospirillaceae/clasificación , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Pradera , Hibridación de Ácido Nucleico , Fosfolípidos/química , Pigmentación , ARN Ribosómico 16S/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADNRESUMEN
Designated strain K5T was isolated from soil on Jeju Island. The bacterium was aerobic, Gram-stain-negative, oxidase-positive, catalase-low activity, motile, short-rod shaped, opaque and formed white colonies that were circular, raised and had entire margins. Strain K5T was able to grow at 15-40 °C, pH 4-9 and at 0-2â% (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequences indicated that strain K5T is closely related to Ferrovibrio soli A15T (98.9â% sequence similarity), Ferrovibrio denitrificans Sp-1T (98.7â%) and Ferrovibrio xuzhouensis LM-6T (97.4â%). The sole respiratory quinone was determined to be ubiquinone-10. The dominant fatty acids of strain K5T were summed feature 8 (C18â:â1 ω7c / C18â:â1ω6c, 29.8â%), C19â:â0 cyclo ω8c (20.2â%) and C16â:â0 (24.4â%). DNA G+C content was 63.6â% and DNA-DNA relatedness between strain K5T and other three members of the genus Ferrovibrio ranged from 24 to 28â%. The major polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine and aminolipids. Moreover, polyphasic characterization revealed that strain K5T represents a novel species in the genus Ferrovibrio, for which the name Ferrovibrio terrae sp. nov. is proposed. The type strain is K5T (=KCCM 43295T=LMG 30611T).
Asunto(s)
Filogenia , Rhodospirillaceae/clasificación , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Islas , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/análogos & derivados , Ubiquinona/químicaRESUMEN
A Gram-stain-negative, strictly aerobic and moderately halophilic bacterium, designated strain Gri0909T, was isolated from a red marine alga, Grinnellia species, in the Yellow Sea of the Republic of Korea. Cells were motile by a single polar flagellum and short-rods showing oxidase and catalase activities. Growth was observed at 10-37 °C (optimum, 30 °C) and pH 6.0-9.0 (optimum, pH 8.0) and in the presence of 0-12.0â% (w/v) NaCl (optimum, 2.0-3.0â%). Strain Gri0909T contained C16â:â0, C18â:â1 2-OH, anteiso-C15â:â0 and summed feature 8 (comprising C18â:â1 ω7c and/or C18â:â1 ω6c) as major fatty acids. Ubiquinone-10 was identified as the sole isoprenoid quinone. Major polar lipids consisted of phosphatidylethanolamine, an identified phospholipid, four unidentified aminolipids and five unidentified lipids. The G+C content of the genomic DNA calculated from the whole-genome sequence was 59.1 mol%. Strain Gri0909T was most closely related to Marivibriohalodurans ZC80T with 91.4â% 16S rRNA gene sequence similarity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Gri0909T formed a phyletic lineage within the family Rhodospirillaceae. The very low 16S rRNA gene sequence similarities, together with distinct phenotypic and chemotaxonomic properties, served to differentiate strain Gri0909T from phylogenetically closely related genera. Here it is proposed that strain Gri0909T represents a new species of a novel genus of the family Rhodospirillaceae, for which the name Hwanghaeella grinnelliae gen. nov., sp. nov. is proposed. The type strain is Gri0909T (=KACC 19793T=JCM 32943T).
Asunto(s)
Filogenia , Rhodophyta/microbiología , Rhodospirillaceae/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
A novel Gram-stain-negative, facultatively anaerobic, oxidase-negative, catalase-positive bacillus, designated WD2A32T, was isolated from a marine solar saltern in the coast of Weihai, Shandong Province, PR China. Strain WD2A32T grew optimally at 37-40 °C (range, 20-45 °C) and pH 7.0-7.5 (range, 6.5-8.0) and was tolerant to 6-8â% (w/v) NaCl (range, 2-12â%). The 16S rDNA of strain WD2A32T was most similar (93.5â%) to that of Rhodovibrio salinarumDSM 9154T (the type species of the genus Rhodovibrio), followed by Limimonas halophilaDSM 25584T (92.4â%; the type species of the genus Limimonas). The similarities to the type strains of the genera Pelagibiusand Limibacilluswere less than 91.0â%. The draft genome sequence of strain WD2A32T contained 72 contigs (>507 bp) of 4â237â996 bp with a DNA G+C content of 65.5 mol%. The major polar lipids of strain WD2A32T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and unidentified aminolipids; major cellular fatty acids were summed feature 8 (C18â:â1ω7c/C18â:â1ω6c) and C19â:â0 cyclo ω8c; the major respiratory quinone was Q-10. On the basis of the results from chemotaxonomic, physiological and biochemical analyses, strain WD2A32T represents a novel species of a novel genus in the family Rhodospirillaceae, for which the name Ferruginivarius sediminum gen. nov., sp. nov. has been proposed. The type strain is WD2A32T (=KCTC 52888T=MCCC 1H00201T).
Asunto(s)
Filogenia , Rhodospirillaceae/clasificación , Salinidad , Microbiología del Agua , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/análogos & derivados , Ubiquinona/químicaAsunto(s)
Infecciones por Bacterias Gramnegativas/diagnóstico , Mastoiditis/microbiología , Rhodospirillaceae/aislamiento & purificación , Anciano , Antibacterianos/uso terapéutico , Técnicas Bacteriológicas , Infecciones por Bacterias Gramnegativas/líquido cefalorraquídeo , Infecciones por Bacterias Gramnegativas/tratamiento farmacológico , Infecciones por Bacterias Gramnegativas/microbiología , Humanos , Masculino , Mastoiditis/líquido cefalorraquídeo , Mastoiditis/diagnóstico por imagen , Mastoiditis/tratamiento farmacológico , Técnicas de Diagnóstico Molecular , ARN Ribosómico 16S/genética , Rhodospirillaceae/genética , Rhodospirillaceae/crecimiento & desarrollo , Agua de Mar/microbiología , Resultado del TratamientoRESUMEN
A Gram-stain-negative, aerobic, flagellated, rod-shaped bacterium, designated strain NAU-10T, was isolated from an oil-contaminated soil collected in PR China. Strain NAU-10T could grow at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (pH 7.0) and in the presence of 0-2.5â% (w/v) NaCl (0.5â% in Luria-Bertani broth). The major fatty acids were C18â:â1 ω7c (38.6â%), C17â:â1 ω6c (9.8â%), C18â:â1 2-OH (9.1â%), summed feature 3 (8.7â%), C16â:â0 3-OH (7.2â%) and C16â:â0 (6.7â%). The major respiratory quinones were Q9 and Q10. The total polar lipids were lipid, aminolipid, phospholipid, phosphatidylglycerol and phosphatidylethanolamine. Strain NAU-10T shared the highest 16S rRNA gene sequence similarities with Rhodocista pekingensis 3-pT (95.9â%), Niveispirillum cyanobacteriorum TH16T (95.3â%) and Niveispirillum fermenti CC-LY736T (95.3â%), and constituted a sub-cluster within the family Rhodospirillaceae. The DNA G+C content of strain NAU-10T was 68.2 mol% based on its draft genome sequence. Genome annotation of strain NAU-10T predicted the presence of 4309 genes, of which 4237 are coding proteins and 72 are RNA genes. Based on its phenotypic and chemotaxonomic characteristics, as well as the analysis of the 16S rRNA gene sequences, it was concluded that strain NAU-10T represents a novel genus, for which the name Oleisolibacter gen. nov., is proposed. The type species of this genus is Oleisolibacter albus with the type strain NAU-10T (=KCTC 62417T=CCTCC AB 2018015T).
Asunto(s)
Contaminación por Petróleo , Filogenia , Rhodospirillaceae/clasificación , Microbiología del Suelo , Contaminantes del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , Quinonas/química , ARN Ribosómico 16S/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADNRESUMEN
A bacterial strain, designated HHTR 118T, was isolated from a culture of the green alga Ulvaprolifera obtained from offshore seawater of Qingdao, Shandong Province, China. Cells of strain HHTR 118T were rod-shaped and motile with a single flagellum, and approximately 0.3-0.4 µm wide and 0.8-1.4 µm long. The strain was Gram-stain-negative, strictly aerobic, catalase-negative and oxidase-positive. Optimal growth was observed at 30 °C, at pH 8.0 and with 1â% (w/v) NaCl. Nitrate was not reduced. Sucrose, sodium citrate and l-leucine stimulated growth, but not lactose, fructose, xylose, d-mannose, glucose, raffinose, rhamnose, ornithine or lysine. The DNA G+C content of strain HHTR 118T calculated on the basis of the genome sequence was 64.9âmol% and the genome size is 4.6 Mbp. The major quinone was ubiquinone 10 and the predominant cellular fatty acids (>10â% of total fatty acids) were summed feature 8 (C18â:â1ω6c and/or C18â:â1ω7c). The predominant polar lipids were phosphatidylglycerol, one unidentified phospholipid, two unidentified aminolipids and three unidentified polar lipids. Phylogenetic analysis, based on 16S rRNA gene sequences, demonstrated that strain HHTR 118T was affiliated with the family Rhodospirillaceae. On the basis of the 16S rRNA gene sequence data as well as physiological and biochemical characteristics, we concluded that strain HHTR 118T represents a novel species of a novel genus. We propose the name of Algihabitans albus gen. nov., sp. nov. for this novel species. The type strain of the novel species is strain HHTR 118T (=KCTC 62395T=MCCC 1K03486T).
Asunto(s)
Filogenia , Rhodospirillaceae/clasificación , Ulva/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , Rhodospirillaceae/aislamiento & purificación , Agua de Mar/microbiología , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
The North China Plain is the agricultural heartland in China. High PM2.5 levels and elevated chemical pollutants have been observed during crop harvest seasons due to open biomass burning. Biomass burning in the wheat-harvest season may significantly deteriorate the regional air quality. The harmful ingredients in smoke particles also have severe implications for toxicity and health effects. Previous studies have illustrated the potential role of bioaerosols as ice-nuclei and cloud condensation nuclei and highlighted their influence on biochemical cycles and human health effects. In a monthly field observation campaign of biomass burning conducted at the summit of Mount Tai in July 2015, we reported the composition, potential role, size distribution of microorganisms in particulate matters PM1.0, PM2.5, and estimated their contribution to particles. The wide-range particle spectrometer suggested that the predominant particles were distributed in submicron particles (PM1.0), which resulted in a similar community structure for bacteria and fungi in PM1.0 and PM2.5. Among bacteria, the predominant Pseudomonas accounted for 18.06% and 21.29% in PM1.0 and PM2.5, respectively. Alternaria covered up to 69.01% and 72.76% of the fungal community in PM1.0 and PM2.5, respectively. A disparity between bacterial communities was identified by the abundance of rare species, such as Bacilli being higher in PM1.0 (2.4%) than in PM2.5 (1.8%), and Defluviicoccus being higher in PM2.5 (2.5%) than in PM1.0 (0.5%), which may be related to cell size and cell growth patterns. Quantitative PCR revealed that microbial cell numbers in PM2.5 were higher than in PM1.0, and that the bacterial cell number was about an order of magnitude greater than the fungal cell number. However, the mass concentration and contribution of fungi to particulate matter was much higher than that of bacteria, suggesting the underestimated role of fungi in atmospheric aerosols. Airborne microorganisms in alpine areas remained less characterized. The findings presented here illustrated the potentially important impacts on air quality and bioaerosol pollution by biomass burning, which provides an essential reference for understanding the transmission and health effects of bioaerosols.
Asunto(s)
Bacterias/aislamiento & purificación , Biomasa , Hongos/aislamiento & purificación , Tamaño de la Partícula , Material Particulado/análisis , Aerosoles/análisis , Agricultura , Contaminantes Atmosféricos/análisis , Contaminación del Aire/análisis , Alternaria/aislamiento & purificación , Bacillus/aislamiento & purificación , Monitoreo del Ambiente/métodos , Incendios , Humanos , Pseudomonas/aislamiento & purificación , Rhodospirillaceae/aislamiento & purificaciónRESUMEN
Sinking marine oil snow was found to be a major mechanism in the transport of spilled oil from the surface to the deep sea following the Deepwater Horizon (DwH) oil spill. Marine snow formation is primarily facilitated by extracellular polymeric substances (EPS), which are mainly composed of proteins and carbohydrates secreted by microorganisms. While numerous bacteria have been identified to degrade oil, there is a paucity of knowledge on bacteria that produce EPS in response to oil and Corexit exposure in the northern Gulf of Mexico (nGoM). In this study, we isolated bacteria from surface water of the nGoM that grow on oil or Corexit dispersant. Among the 100 strains isolated, nine were identified to produce remarkable amounts of EPS. 16S rRNA gene analysis revealed that six isolates (strains C1, C5, W10, W11, W14, W20) belong to the genus Alteromonas; the others were related to Thalassospira (C8), Aestuariibacter (C12), and Escherichia (W13a). The isolates preferably degraded alkanes (17-77%), over polycyclic aromatic hydrocarbons (0.90-23%). The EPS production was determined in the presence of a water accommodated fraction (WAF) of oil, a chemical enhanced WAF (CEWAF), Corexit, and control. The highest production of visible aggregates was found in Corexit followed by CEWAF, WAF, and control; indicating that Corexit generally enhanced EPS production. The addition of WAF and Corexit did not affect the carbohydrate content, but significantly increased the protein content of the EPS. On the average, WAF and CEWAF treatments had nine to ten times more proteins, and Corexit had five times higher than the control. Our results reveal that Alteromonas and Thalassospira, among the commonly reported bacteria following the DwH spill, produce protein rich EPS that could have crucial roles in oil degradation and marine snow formation. This study highlights the link between EPS production and bacterial oil-degrading capacity that should not be overlooked during spilled oil clearance.
Asunto(s)
Bacterias/clasificación , Matriz Extracelular de Sustancias Poliméricas/microbiología , Sedimentos Geológicos/microbiología , Contaminación por Petróleo/análisis , Alteromonas/clasificación , Alteromonas/aislamiento & purificación , Bacterias/aislamiento & purificación , Bacterias/metabolismo , Biodegradación Ambiental , Carbohidratos/análisis , ADN Bacteriano/genética , ADN Ribosómico/genética , Matriz Extracelular de Sustancias Poliméricas/metabolismo , Golfo de México , Filogenia , ARN Ribosómico 16S/genética , Rhodospirillaceae/clasificación , Rhodospirillaceae/aislamiento & purificaciónRESUMEN
A novel basophilic bacterial strain, designated as SCSIO 08040T, was recovered from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-negative, vibrioid or spiral, light pink, 0.6-1.0 µm wide and 1.0-2.5 µm long. Growth occurred at 20-45 °C, pH 7-11 and <5â% (w/v) NaCl, with optimum growth at 28-37 °C, pH 7 and 0-3â% (w/v) NaCl. Catalase-, oxidase and urease-positive, nitrate reduction-negative. Analysis of 16S rRNA gene sequencing revealed that strain SCSIO 08040T had the highest similarity of 95.3â% to Rhodocista pekingensis 3-pT. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences showed that the novel isolate formed a distinct phylogenetic lineage in the family Rhodospirillaceae. The whole-cell hydrolysate contained meso-diaminopimelic acid, galactose, mannose and xylose. The total cellular fatty acid profile was dominated by C18:1ω7c and C19:0cycloω8c. Q-10 was the predominant ubiquinone. The major phospholipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The DNA G+C content of strain SCSIO 08040T was 66.82 mol%. Based on these polyphasic data, a new genus, Indioceanicola gen. nov., is proposed in the family Rhodospirillaceae with the type species Indioceanicola profundi sp. nov. and the type strain SCSIO 08040T (=DSM 105146T=CGMCC 1.15812T).
Asunto(s)
Sedimentos Geológicos/microbiología , Filogenia , Rhodospirillaceae/clasificación , Agua de Mar/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Océano Índico , Fosfolípidos/química , Pigmentación , ARN Ribosómico 16S/genética , Rhodospirillaceae/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/análogos & derivados , Ubiquinona/químicaRESUMEN
A clinical case study involving a man (35-49 years of age) with wounds to his lower right extremity. An isolate was sent to the Delaware Public Health Laboratory for confirmatory testing by genetic analysis of the 16S gene. Testing identified the isolate as a novel genus and species, Haematospirillum jordaniae.
Asunto(s)
Infecciones por Bacterias Gramnegativas/diagnóstico , Infecciones por Bacterias Gramnegativas/microbiología , Rhodospirillaceae/clasificación , Adulto , Antibacterianos/uso terapéutico , Técnicas Bacteriológicas , Infecciones por Bacterias Gramnegativas/tratamiento farmacológico , Humanos , Masculino , Persona de Mediana Edad , ARN Ribosómico 16S/genética , Rhodospirillaceae/genética , Rhodospirillaceae/aislamiento & purificación , Resultado del Tratamiento , Infección de Heridas/diagnóstico , Infección de Heridas/tratamiento farmacológico , Infección de Heridas/microbiologíaAsunto(s)
Fascitis Necrotizante/diagnóstico , Infecciones por Bacterias Gramnegativas/diagnóstico , Rhodospirillaceae/aislamiento & purificación , Anciano , Antibacterianos/uso terapéutico , Diagnóstico Diferencial , Fascitis Necrotizante/complicaciones , Infecciones por Bacterias Gramnegativas/complicaciones , Infecciones por Bacterias Gramnegativas/tratamiento farmacológico , Humanos , Masculino , Filogenia , ARN Ribosómico 16S/química , ARN Ribosómico 16S/genética , ARN Ribosómico 16S/metabolismo , Rhodospirillaceae/clasificación , Rhodospirillaceae/genética , Sepsis/diagnóstico , Análisis de Secuencia de ADNRESUMEN
Two novel marine bacteria, designated strains CSC3H3T and CSC1P2, were isolated from surface seawater of the South China Sea. Both strains were Gram-negative, oxidase-positive, catalase-positive, curved rods and motile. They grew at 10-40 °C, pH 5-10 and in the presence of 0-15â% (w/v) NaCl. Their 16S rRNA gene sequences were identical to each other. Phylogenetic analysis based on 16S rRNA gene sequences indicated that they belong to the genus Thalassospira, and shared 97.5-98.3â% sequence similarity to all other validly type strains of the genus Thalassospira, and the highest similarity was to the type strain Thalassospira povalilyticaZumi 95T (98.3â%), followed by Thalassospira australica NP3b2T (98.2â%). The digital DNA-DNA hybridization value between the two strains was 80.4â%, while the values with T. povalilyticaZumi 95T and T. australica NP3b2T were only 20.5-20.7â% and 20.4-20.5â%, respectively. The two strains possess similar major cellular fatty acids including C18â:â1ω7c, C16â:â0, C19â:â0ω8c cyclo, C18â:â1 2-OH and C17â:â0 cyclo. The G+C contents of the chromosomal DNA of strains CSC3H3T and CSC1P2 were 54.6 and 54.5 mol%, respectively. The major respiratory quinone was ubiquinone 10. Phosphatidylethanolamine, phosphatidylglycerol and several unidentified phospholipids, aminolipid and lipids were present in both strains. Based on phenotypic and genotypic characteristics, the two strains represent a novel species within the genus Thalassospira, for which the name Thalassospira marina sp. nov. is proposed. The type strain is CSC3H3T (=MCCC 1A11786T=KCTC 62333T).
Asunto(s)
Filogenia , Rhodospirillaceae/clasificación , Agua de Mar/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , Rhodospirillaceae/genética , Rhodospirillaceae/aislamiento & purificación , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
Nitrospirillum amazonense is a nitrogen-fixing bacterium that shows potential to promote plant growth when inoculated into sugarcane and rice plants. This microorganism has been the subject of biochemical and genetic characterization to elucidate important functions related to host plant interaction and growth promotion, including the determination of draft genome sequences of two strains, Y2 and CBAmC, the second of which is the aim of the present study. CBAmC has been isolated from sugarcane (Saccharum spp.), and is currently used in a sugarcane consortium inoculant with four other nitrogen-fixing bacterial strains. The present paper describes a significant improvement in the genome sequence and assembly for the N. amazonense strain CBAmC, and determination for the first time of a complete genome sequence for this bacterial species, using PacBio technology. The analysis of the genomic data obtained allowed the discovery of genes coding for metabolic pathways and cellular structures that may be determinant for the success of the bacterial establishment and colonization into the host sugarcane plant, besides conferring important characteristics to the inoculant. These include genes for the use of sucrose and N-glycans, biosynthesis of autoinducer molecules, siderophore production and acquisition, auxin and polyamine biosynthesis, flagellum, σ-fimbriae, a variety of secretion systems, and a complete denitrification system. Concerning genes for nitrogenase and auxiliary proteins, it was possible to corroborate literature data that in N. amazonense these probably had originated from horizontal gene transfer, from bacteria of the Rhizobiales order. The complete genomic sequence of the CBAmC strain of N. amazonense revealed that the bacterium harbors four replicons, including three chromosomes and one chromid, a profile that coincides with that of other two strains, according to literature data, suggesting that as a replicon pattern for the species. Finally, results of phylogenomic analyses in this work support the recent reclassification of the species, separating it from the Azospirillum genus. More importantly, results of the present work shall guide subsequent studies on strain CBAmC as well as the development of a sugarcane inoculant.
Asunto(s)
Genoma Bacteriano , Fijación del Nitrógeno , Tallos de la Planta/microbiología , Rhodospirillaceae/genética , Saccharum/microbiología , Rhodospirillaceae/aislamiento & purificaciónRESUMEN
A novel bacterial strain MAH-8T was isolated from a soil sample of a Korean pine garden and was characterized using a polyphasic approach. Cells were Gram-staining negative, pinkish yellow colored, motile and vibrio-shaped. The strain was aerobic and catalase, oxidase positive, optimum growth temperature and pH were 28-30 °C and 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain MAH-8T belongs to the genus Caenispirillum and is most closely related to Caenispirillum bisanense KCTC 12839T (98.14%), Caenispirillum deserti KCTC 42064T (96.35%), and Caenispirillum salinarum JCM 17360T (95.76%). In DNA-DNA hybridization tests, the DNA relatedness between strain MAH-8T and its closest phylogenetic neighbor was below 45.0%. The DNA G + C content was 70.5 mol% and the predominant respiratory quinone was ubiquinone-10. Flexirubin-type pigments were present and the major cellular fatty acids were C18:1 ω7c/C18:1 ω6c, C16:1 ω7c/C16:1 ω6c and C16:0. The results of DNA-DNA hybridization and genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain MAH-8T represented a novel species within the genus Caenispirillum, for which the name Caenispirillum humi, is proposed. The type strain is MAH-8T (= KACC 19294T = CGMCC 1.16224T). The NCBI GenBank Accession Number for the 16S rRNA gene sequence of strain MAH-8T is KY964275.