Asunto(s)
Ataxia/fisiopatología , Lipomatosis/diagnóstico por imagen , Síndrome MERRF/fisiopatología , Mioclonía/fisiopatología , Anciano , ADN Mitocondrial/genética , Pruebas Genéticas , Humanos , Síndrome MERRF/diagnóstico , Síndrome MERRF/genética , Imagen por Resonancia Magnética , Masculino , CuelloRESUMEN
Neuropathies in Myoclonic Epilepsy with Ragged Red Fibers (MERRF) syndrome are frequent but ganglionopathies have never been reported. We retrospectively identified 24 patients with MERRF mutations in the neuromuscular center Nord/Est/Ile de France (Pitié-Salpêtrière, Paris, France). Seventeen nerve conduction studies (NCS) were available. Five patients had MERRF syndrome and ganglionopathy, a pure sensory neuropathy. All of them displayed ataxia and mild clinical sensory abnormalities. Ganglionopathies have been reported in mitochondrial diseases but never in MERRF syndrome. We suggest that patients presenting with ganglionopathy, especially if associated with myopathy, lipomatosis or epilepsy, should be screened for MERRF mutations.
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Ataxia/fisiopatología , Ganglios Espinales/fisiopatología , Síndrome MERRF/fisiopatología , Enfermedades del Sistema Nervioso Periférico/fisiopatología , Trastornos de la Sensación/fisiopatología , Adulto , Ataxia/diagnóstico , Ataxia/etiología , Humanos , Síndrome MERRF/complicaciones , Síndrome MERRF/genética , Conducción Nerviosa/fisiología , Enfermedades del Sistema Nervioso Periférico/diagnóstico , Enfermedades del Sistema Nervioso Periférico/etiología , Estudios Retrospectivos , Trastornos de la Sensación/diagnóstico , Trastornos de la Sensación/etiologíaRESUMEN
Myoclonus epilepsy associated with ragged-red fibers (MERRF) is traditionally characterized by myoclonus, generalized epilepsy and ragged-red fibers. We herein report a 42-year-old man who complained of falling after starting running, symptoms resembling those of paroxysmal kinesigenic dyskinesia. He showed only slight muscle weakness of the right quadriceps femoris. Muscle pathology and a genetic analysis identified him as having MERRF with a 8344A>G mtDNA mutation. We diagnosed his symptoms as having been caused by slight quadriceps femoris muscle weakness and exercise intolerance. This case suggests that mitochondrial myopathy should be considered in cases with strong muscle symptoms for muscle weakness.
Asunto(s)
Accidentes por Caídas , ADN Mitocondrial/genética , Síndrome MERRF/diagnóstico , Síndrome MERRF/genética , Debilidad Muscular/etiología , Mutación Puntual , Carrera/lesiones , Adulto , Diagnóstico Diferencial , Distonía/diagnóstico , Tolerancia al Ejercicio , Pruebas Genéticas , Humanos , Síndrome MERRF/fisiopatología , Masculino , Músculo Esquelético/fisiopatologíaRESUMEN
BACKGROUND: Given the etiologic heterogeneity of disease classification using clinical phenomenology, we employed contemporary criteria to classify variants associated with myoclonic epilepsy with ragged-red fibers (MERRF) syndrome and to assess the strength of evidence of gene-disease associations. Standardized approaches are used to clarify the definition of MERRF, which is essential for patient diagnosis, patient classification, and clinical trial design. METHODS: Systematic literature and database search with application of standardized assessment of gene-disease relationships using modified Smith criteria and of variants reported to be associated with MERRF using modified Yarham criteria. RESULTS: Review of available evidence supports a gene-disease association for two MT-tRNAs and for POLG. Using modified Smith criteria, definitive evidence of a MERRF gene-disease association is identified for MT-TK. Strong gene-disease evidence is present for MT-TL1 and POLG. Functional assays that directly associate variants with oxidative phosphorylation impairment were critical to mtDNA variant classification. In silico analysis was of limited utility to the assessment of individual MT-tRNA variants. With the use of contemporary classification criteria, several mtDNA variants previously reported as pathogenic or possibly pathogenic are reclassified as neutral variants. CONCLUSIONS: MERRF is primarily an MT-TK disease, with pathogenic variants in this gene accounting for ~90% of MERRF patients. Although MERRF is phenotypically and genotypically heterogeneous, myoclonic epilepsy is the clinical feature that distinguishes MERRF from other categories of mitochondrial disorders. Given its low frequency in mitochondrial disorders, myoclonic epilepsy is not explained simply by an impairment of cellular energetics. Although MERRF phenocopies can occur in other genes, additional data are needed to establish a MERRF disease-gene association. This approach to MERRF emphasizes standardized classification rather than clinical phenomenology, thus improving patient diagnosis and clinical trial design.
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Síndrome MERRF , Humanos , Síndrome MERRF/clasificación , Síndrome MERRF/diagnóstico , Síndrome MERRF/genética , Síndrome MERRF/fisiopatologíaRESUMEN
Mitochondrial disorders is a group of clinical entities associated with abnormalities of the mitochondrial respiratory chain (MRC), which carries out the oxidative phosphorylation (OXPHOS) of ADP into ATP. As the MRC is the result of genetic complementation between two separate genomes, nuclear and mitochondrial, OXPHOS failure can derive from mutations in either nuclear-encoded, or mitochondrial-encoded, genes. Epilepsy is a relatively common feature of mitochondrial disease, especially in early-onset encephalopathies of infants and children. However, the two most common entities associated with epilepsy include MERRF, for Myoclonic Epilepsy with Ragged Red Fibers, and AHS, or Alpers-Huttenlocher syndrome, also known as hepatopathic poliodystrophy. Whilst MERRF is a maternally inherited condition caused by mtDNA mutations, particularly the 8344A>G substitution in the gene encoding mt-tRNALys, AHS is typically caused by recessive mutations in POLG, encoding the catalytic subunit of polymerase gamma, the only mtDNA polymerase in humans. AHS is the most severe, early-onset, invariably fatal syndrome within a disease spectrum, which also include other epileptogenic entities, all due to POLG mutations and including Spino-cerebellar Ataxia and Epilepsy (SCAE). This review reports the main clinical, neuroimaging, biochemical, and molecular features of epilepsy-related mitochondrial syndrome, particularly MERRF and AHS.
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Esclerosis Cerebral Difusa de Schilder/genética , Esclerosis Cerebral Difusa de Schilder/fisiopatología , Síndrome MERRF/genética , Síndrome MERRF/fisiopatología , Esclerosis Cerebral Difusa de Schilder/terapia , Humanos , Síndrome MERRF/terapiaRESUMEN
The m.8344A>G mutation in the MTTK gene, which encodes the mitochondrial transfer RNA for lysine, is traditionally associated with myoclonic epilepsy and ragged-red fibres (MERRF), a multisystemic mitochondrial disease that is characterised by myoclonus, seizures, cerebellar ataxia, and mitochondrial myopathy with ragged-red fibres. We studied the clinical and paraclinical phenotype of 34 patients with the m.8344A>G mutation, mainly derived from the nationwide mitoREGISTER, the multicentric registry of the German network for mitochondrial disorders (mitoNET). Mean age at symptom onset was 24.5 years ±10.9 (6-48 years) with adult onset in 75 % of the patients. In our cohort, the canonical features seizures, myoclonus, cerebellar ataxia and ragged-red fibres that are traditionally associated with MERRF, occurred in only 61, 59, 70, and 63 % of the patients, respectively. In contrast, other features such as hearing impairment were even more frequently present (72 %). Other common features in our cohort were migraine (52 %), psychiatric disorders (54 %), respiratory dysfunction (45 %), gastrointestinal symptoms (38 %), dysarthria (36 %), and dysphagia (35 %). Brain MRI revealed cerebral and/or cerebellar atrophy in 43 % of our patients. There was no correlation between the heteroplasmy level in blood and age at onset or clinical phenotype. Our findings further broaden the clinical spectrum of the m.8344A>G mutation, document the large clinical variability between carriers of the same mutation, even within families and indicate an overlap of the phenotype with other mitochondrial DNA-associated syndromes.
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Síndrome MERRF/genética , Síndrome MERRF/fisiopatología , Mutación , ARN de Transferencia de Lisina/genética , ARN/genética , Adolescente , Adulto , Edad de Inicio , Anciano , Encéfalo/diagnóstico por imagen , Estudios de Cohortes , Femenino , Alemania/epidemiología , Humanos , Síndrome MERRF/tratamiento farmacológico , Síndrome MERRF/epidemiología , Masculino , Persona de Mediana Edad , Linaje , Fenotipo , ARN Mitocondrial , Sistema de RegistrosRESUMEN
Mitochondrial disorders (MD) represent a clinically, biochemically and genetically heterogeneous group of diseases associated with dysfunction of the oxidative phosphorylation system and pyruvate dehydrogenase complex. Our aim was to illustrate the most common clinical presentation of MD on the example of selected diseases and syndromes. The minimal prevalence of MD is estimated as 1 to 5,000. MD may manifest at any age since birth until late-adulthood with acute manifestation or as a chronic progressive disease. Virtually any organ may be impaired, but the organs with the highest energetic demands are most frequently involved, including brain, muscle, heart and liver. Some MD may manifest as a characteristic cluster of clinical features (e.g. MELAS syndrome, Kearns-Sayre syndrome). Diagnostics includes detailed history, the comprehensive clinical examination, results of specialized examinations (especially cardiology, visual fundus examination, brain imaging, EMG), laboratory testing of body fluids (lactate, aminoacids, organic acids), and analysis of bioptic samples of muscle, skin, and liver, eventually. Normal lactate level in blood does not exclude the possibility of MD. Although the aimed molecular genetic analyses may be indicated in some of mitochondrial diseases, the methods of next generation sequencing come into focus. Examples of treatment are arginine supplementation in MELAS syndrome, ketogenic diet in pyruvate oxidation disorders or quinone analogs in patients with LHON. Conclusion: The clinical suspicion of a mitochondrial disorder is often delayed, or the disease remains undiagnosed. The correct diagnosis and adequate treatment can improve prognosis of the patient. Access to genetic counseling is also of great importance.
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Encéfalo/fisiopatología , Enfermedades Mitocondriales/diagnóstico , Enfermedades Mitocondriales/fisiopatología , ADN Mitocondrial/análisis , Electroencefalografía , Humanos , Síndrome de Kearns-Sayre/diagnóstico , Síndrome de Kearns-Sayre/fisiopatología , Síndrome MELAS/diagnóstico , Síndrome MELAS/fisiopatología , Síndrome MERRF/diagnóstico , Síndrome MERRF/fisiopatología , Encefalomiopatías Mitocondriales/diagnóstico , Encefalomiopatías Mitocondriales/fisiopatología , Miopatías Mitocondriales/diagnóstico , Miopatías Mitocondriales/fisiopatologíaAsunto(s)
Enfermedad de Leigh , Síndrome MERRF , Enfermedades Talámicas , Adolescente , Humanos , Enfermedad de Leigh/genética , Enfermedad de Leigh/patología , Enfermedad de Leigh/fisiopatología , Síndrome MERRF/genética , Síndrome MERRF/patología , Síndrome MERRF/fisiopatología , Masculino , Mutación , Fenotipo , Enfermedades Talámicas/genética , Enfermedades Talámicas/patología , Enfermedades Talámicas/fisiopatologíaRESUMEN
OBJECTIVES: Myoclonic epilepsy with ragged-red fibers (MERRF) is a rare mitochondrial syndrome, mostly caused by the 8344A>G mitochondrial DNA mutation. Most of the previous studies have been based on single case/family reports or series with few patients. The primary aim of this study was the characterization of a large cohort of patients with the 8344A>G mutation. The secondary aim was revision of the previously published data. METHODS: Retrospective, database-based study (Nation-wide Italian Collaborative Network of Mitochondrial Diseases) and systematic revision. RESULTS: Forty-two patients carrying the mutation were identified. The great majority did not have full-blown MERRF syndrome. Myoclonus was present in 1 of 5 patients, whereas myopathic signs and symptoms, generalized seizures, hearing loss, eyelid ptosis, and multiple lipomatosis represented the most common clinical features. Some asymptomatic mutation carriers have also been observed. Myoclonus was more strictly associated with ataxia than generalized seizures in adult 8344A>G subjects. Considering all of the 321 patients so far available, including our dataset and previously published cases, at the mean age of approximately 35 years, the clinical picture was characterized by the following signs/symptoms, in descending order: myoclonus, muscle weakness, ataxia (35%-45% of patients); generalized seizures, hearing loss (25%-34.9%); cognitive impairment, multiple lipomatosis, neuropathy, exercise intolerance (15%-24.9%); and increased creatine kinase levels, ptosis/ophthalmoparesis, optic atrophy, cardiomyopathy, muscle wasting, respiratory impairment, diabetes, muscle pain, tremor, migraine (5%-14.9%). CONCLUSIONS: Our results showed higher clinical heterogeneity than commonly thought. Moreover, MERRF could be better defined as a myoclonic ataxia rather than a myoclonic epilepsy.
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ADN Mitocondrial/genética , Síndrome MERRF/genética , Síndrome MERRF/fisiopatología , Mutación/genética , Fenotipo , Adulto , Edad de Inicio , Bases de Datos Genéticas , Progresión de la Enfermedad , Femenino , Humanos , Síndrome MERRF/patología , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Estudios RetrospectivosRESUMEN
Mitochondrial diseases are a diverse group of inherited and acquired disorders that result in inadequate energy production. They can be caused by inheritable genetic mutations, acquired somatic mutations, and exposure to toxins (including some prescription medications). Normal mitochondrial physiology is responsible, in part, for the aging process itself, as free radical production within the mitochondria results in a lifetime burden of oxidative damage to DNA, especially the mitochondrial DNA that, in turn, replicate the mutational burden in future copies of itself, and lipid membranes. Primary mitochondrial diseases are those caused by mutations in genes that encode for mitochondrial structural and enzymatic proteins, and those proteins required for mitochondrial assembly and maintenance. A number of common adult maladies are associated with defective mitochondrial energy production and function, including diabetes, obesity, hyperthyroidism, hypothyroidism, and hyperlipidemia. Mitochondrial dysfunction has been demonstrated in many neurodegenerative disorders, including Alzheimer's disease, Parkinson disease, amyotrophic lateral sclerosis, and some cancers. Polymorphisms in mitochondrial DNA have been linked to disease susceptibility, including death from sepsis and survival after head injury. There is considerable overlap in symptoms caused by primary mitochondrial diseases and those illnesses that affect mitochondrial function, but are not caused by primary mutations, as well as disorders that mimic mitochondrial diseases, but are caused by other identified mutations. Evaluation of these disorders is complex, expensive, and not without false-negative and false-positive results that can mislead the physician. Most of the common heritable mitochondrial disorders have been well-described in the literature, but can be overlooked by many clinicians if they are uneducated about these disorders. In general, the evaluation of the classic mitochondrial disorders has become straightforward if the clinician recognized the phenotype and orders appropriate confirmatory testing. However, the majority of patients referred for a mitochondrial evaluation do not have a clear presentation that allows for rapid identification and testing. This article provides introductory comments on mitochondrial structure, physiology, and genetics, but will focus on the presentation and evaluation of adults with mitochondrial symptoms, but who may not have a primary mitochondrial disease.
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Síndrome MELAS/diagnóstico , Síndrome MELAS/fisiopatología , Síndrome MERRF/diagnóstico , Síndrome MERRF/fisiopatología , Enfermedades Mitocondriales/diagnóstico , Enfermedades del Sistema Nervioso/diagnóstico , Adulto , Humanos , Síndrome MELAS/genética , Síndrome MELAS/terapia , Síndrome MERRF/genética , Síndrome MERRF/terapia , Mitocondrias/genética , Mitocondrias/patología , Enfermedades Mitocondriales/fisiopatología , Enfermedades del Sistema Nervioso/fisiopatología , Medicina de Precisión , Terminología como AsuntoRESUMEN
The mitochondrial respiratory chain is the final common pathway for energy production. Defects affecting this pathway can give rise to disease that presents at any age and affects any tissue. However, irrespective of genetic defect, epilepsy is common and there is a significant risk of status epilepticus. This review summarizes our current understanding of the epilepsy that occurs in mitochondrial disease, focusing on three of the most common disorders: mitochondrial myopathy encephalopathy, lactic acidosis and stroke-like episodes (MELAS), myoclonus epilepsy and ragged-red fibers (MERRF), and polymerase gamma (POLG) related disease. In addition, we review the pathogenesis and possible treatment of these disorders.
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Epilepsia/etiología , Enfermedades Mitocondriales/complicaciones , Anticonvulsivantes/uso terapéutico , Epilepsia/fisiopatología , Epilepsia/terapia , Humanos , Síndrome MERRF/patología , Síndrome MERRF/fisiopatología , Mitocondrias/fisiología , Enfermedades Mitocondriales/fisiopatología , Enfermedades Mitocondriales/terapia , Encefalomiopatías Mitocondriales/complicaciones , Encefalomiopatías Mitocondriales/patologíaRESUMEN
Mitochondrial DNA mutations are an important cause of human disease for which there is no effective treatment. Myoclonic epilepsy with ragged-red fibers (MERRF) is a mitochondrial disease usually caused by point mutations in transfer RNA genes encoded by mitochondrial DNA. The most common mutation associated with MERRF syndrome, m.8344A > G in the gene MT-TK, which encodes transfer RNA(Lysine), affects the translation of all mitochondrial DNA encoded proteins. This impairs the assembly of the electron transport chain complexes leading to decreased mitochondrial respiratory function. Here we report on how this mutation affects mitochondrial function in primary fibroblast cultures established from patients harboring the A8344G mutation. Coenzyme Q10 levels, as well as mitochondrial respiratory chain activity, and mitochondrial protein expression levels were significantly decreased in MERRF fibroblasts. Mitotracker staining and imaging analysis of individual mitochondria indicated the presence of small, rounded, depolarized mitochondria in MERRF fibroblasts. Mitochondrial dysfunction was associated with increased oxidative stress and increased degradation of impaired mitochondria by mitophagy. Transmitochondrial cybrids harboring the A8344G mutation also showed CoQ10 deficiency, mitochondrial dysfunction, and increased mitophagy activity. All these abnormalities in patient-derived fibroblasts and cybrids were partially restored by CoQ10 supplementation, indicating that these cell culture models may be suitable for screening and validation of novel drug candidates for MERRF disease.
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Fibroblastos/patología , Síndrome MERRF/patología , Síndrome MERRF/fisiopatología , Ubiquinona/análogos & derivados , Línea Celular , Células Cultivadas , Fibroblastos/fisiología , Humanos , Síndrome MERRF/genética , Potencial de la Membrana Mitocondrial/genética , Mutación/genética , Ubiquinona/fisiologíaRESUMEN
Myoclonic epilepsy and ragged-red fibers (MERRF) syndrome is a rare disorder characterized by myoclonus, muscle weakness, cerebellar ataxia, heart conduction block, and dementia. It has been documented that 80-90% of the patients with MERRF syndrome are caused by the A8344G mutation in the tRNA(Lys) gene of mitochondrial DNA (mtDNA). We and other investigators have reported that the mtDNA mutation results in not only inefficient generation of adenosine triphosphate but also increased production of reactive oxygen species (ROS) in cultured cells harboring A8344G mutation of mtDNA. In addition, we found an imbalance in the gene expression of antioxidant enzymes in the skin fibroblasts of MERRF patients. The mRNA, protein, and enzyme activity levels of manganese-superoxide dismutase were increased, but those of Cu,Zn-SOD, catalase, and glutathione peroxidase did not show significant changes. Recently, we showed that the excess ROS could damage voltage-dependent anion channel, prohibitin, Lon protease, and aconitase in the MERRF cells. Moreover, there was a dramatic increase in the gene expression and activity of matrix metalloproteinase 1, which may contribute to the cytoskeleton remodeling involved in the weakness and atrophy of muscle commonly seen in MERRF patients. Taken together, we suggest that mtDNA mutation-elicited oxidative stress, oxidative damage, and altered gene expression are involved in the pathogenesis and progression of MERRF syndrome.
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Daño del ADN , ADN Mitocondrial , Expresión Génica , Síndrome MERRF/genética , Mutación , Estrés Oxidativo/genética , Antioxidantes/metabolismo , Respiración de la Célula/fisiología , Células Cultivadas , Citoesqueleto/metabolismo , ADN Mitocondrial/genética , ADN Mitocondrial/metabolismo , Fibroblastos/citología , Fibroblastos/fisiología , Humanos , Síndrome MERRF/patología , Síndrome MERRF/fisiopatología , Metaloproteinasa 1 de la Matriz/metabolismo , Enfermedades Mitocondriales/genética , Enfermedades Mitocondriales/patología , Enfermedades Mitocondriales/fisiopatología , Proteínas Mitocondriales/genética , Proteínas Mitocondriales/metabolismo , Fosforilación Oxidativa , Especies Reactivas de Oxígeno/metabolismoRESUMEN
This study was conducted to investigate the etiology, the clinical characteristics and prognosis of acute necrotizing encephalopathy (ANE) in Korean children. Six children (1 yr to 7 yr) patients with ANE were enrolled. They were diagnosed by clinical and radiological characteristics and their clinical data were retrospectively analyzed. In a search of clinically plausible causes, brain MRI in all patients, mitochondrial DNA studies for mitochondrial encephalomyopathy, lactic acidosis, and strokelike episodes (MELAS) and myoclonus epilepsy and ragged red fibers (MERRF) in four patients, and genomic typing on HLA DRB/HLA DQB genes in three patients were performed. All had precedent illnesses and the main initial symptoms included mental change (83%), seizures (50%), and focal deficits (50%). MRI revealed increased T2 signal density in the bilateral thalami and/or the brainstem in all patients. Mitochodrial DNA studies for MELAS and MERRF were negative in those children and HLA-DRB1*1401, HLA-DRB3*0202, and HLA-DQB1*0502 seemed to be significant. A high dose steroid was given to all patients, which seemed to be partly effective except for 2 patients. In conclusion, ANE is relatively rare, but can result in serious neurological complication in children. Early detection and appropriate treatment may lead to a better neurological outcome.
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Leucoencefalitis Hemorrágica Aguda/patología , Leucoencefalitis Hemorrágica Aguda/fisiopatología , Niño , Preescolar , Femenino , Antígenos HLA-DQ/metabolismo , Cadenas beta de HLA-DQ , Antígenos HLA-DR/metabolismo , Cadenas HLA-DRB1 , Cadenas HLA-DRB3 , Humanos , Lactante , Corea (Geográfico) , Leucoencefalitis Hemorrágica Aguda/diagnóstico , Leucoencefalitis Hemorrágica Aguda/etiología , Síndrome MELAS/patología , Síndrome MELAS/fisiopatología , Síndrome MERRF/patología , Síndrome MERRF/fisiopatología , Imagen por Resonancia Magnética , Masculino , Pronóstico , Estudios RetrospectivosAsunto(s)
Infartos del Tronco Encefálico/genética , Predisposición Genética a la Enfermedad/genética , Síndrome MERRF/complicaciones , Síndrome MERRF/genética , Mutación/genética , ARN de Transferencia de Lisina/genética , Adolescente , Ataxia/genética , Tronco Encefálico/patología , Tronco Encefálico/fisiopatología , Infartos del Tronco Encefálico/patología , Infartos del Tronco Encefálico/fisiopatología , Análisis Mutacional de ADN , Trastornos de Deglución/genética , Progresión de la Enfermedad , Disartria/genética , Femenino , Marcadores Genéticos/genética , Pruebas Genéticas , Genotipo , Humanos , Patrón de Herencia/genética , Síndrome MERRF/fisiopatología , Imagen por Resonancia Magnética , Recurrencia , Síndrome de Dificultad Respiratoria/genéticaRESUMEN
MERRF is an acronym of myoclonus epilepsy associated with ragged-red fibers and was first reported as a new nosological entity belonging to mitochondrial encephalomyopathies in San Remo symposium on "Mitochondrial Pathology" in 1982. MERRF was named Fukuhara disease by Rowland (1983). The first reported patient had been diagnosed as having Ramsay Hunt syndrome associated with Friedreich's ataxia. However, nowadays, the previously reported cases as having Ramsay Hunt syndrome associated with Friedreich's ataxia are regarded as having been suffered from MERRF. The history in establishing the nosological entity of MERRF was described. Patients with MERRF develop myoclonus, epileptic seizures, cerebellar ataxia, dementia, sensorineural hearing disturbance, optic atrophy, muscular wasting, and foot deformities at the advanced stage. Pathological findings show degeneration of the dentate nuclei, globus pallidus, and red nuclei, substantia nigra, inferior olivary nuclei, cerebellar cortex, and spinal cord. The posterior columns, the spinocerebellar tracts, and Clark's columns are degenerating in the spinal cord. The pyramidal tracts never show a severe degeneration as in Friedreich's ataxia. The skeletal muscles show mitochondrial abnormalities histologically and electron microscopically. Clinical features of MERRF are not necessarily uniform in the early stage and muscle biopsy findings are also very mild in some patients with MERRF, necessitating genetic analysis for diagnosis. Most of patients show a point mutation (A --> G) of nt 8344 in mitochondrial DNA.
Asunto(s)
Síndrome MERRF , Adulto , Sistema Nervioso Central/patología , ADN Mitocondrial/genética , Epilepsias Mioclónicas , Femenino , Humanos , Japón , Síndrome MERRF/diagnóstico , Síndrome MERRF/genética , Síndrome MERRF/patología , Síndrome MERRF/fisiopatología , Masculino , Mitocondrias Musculares/patología , Encefalomiopatías Mitocondriales , Mutación PuntualRESUMEN
The characteristic clinical presentation, especially the appearance of muscle symptoms, is quite unique in children carrying the mtA8344G mutation. The diagnosis of MERRF syndrome is seldom made in the pediatric age. Fatigue is a common finding in children of pubertal age. Fatigue in combination with recurrent resting muscle pain occurs frequently in the initial phase of various hereditary muscle disorders and in several autoimmune, endocrine and metabolic syndromes. In the absence of obvious biochemical/metabolic abnormalities and in the lack of neurological symptoms the complaints are frequently labelled as fibromyalgia or chronic fatigue syndrome. In patients with behavioural or psychiatric abnormalities one might even start to question the organic etiology of the complaints. We describe a family carrying the classic MTTK mutation with a variable degree of heteroplasmy, presenting in childhood as isolated recurrent muscle pain as the first symptom of the disease.
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Síndrome MERRF/complicaciones , Síndrome MERRF/genética , Síndrome MERRF/fisiopatología , Enfermedades Musculares/etiología , Dolor/etiología , Adolescente , Adulto , Niño , Análisis Mutacional de ADN , ADN Mitocondrial/genética , Femenino , Humanos , Enfermedades Musculares/genética , Enfermedades Musculares/fisiopatología , Mutación , Dolor/genética , Dolor/fisiopatología , Linaje , Reacción en Cadena de la PolimerasaRESUMEN
The treatment of progressive myoclonic epilepsy (PME) is largely empirical, even though valproic acid (VPA) is usually considered the drug of first choice. However, VPA should be used with caution in PME due to mitochondrial dysfunction, i.e. in MERRF (myoclonic epilepsy with ragged red fibers) syndrome, because of its interaction with mitochondrial respiration and metabolism. Levetiracetam (LEV) treatment was started in combination with VPA in a patient with typical clinical, histological, and biochemical features of MERRF due to a mutation on the tRNA of Phenilalanine gene. The average myoclonus score improved dramatically, as well as the quality of life and no side effects were observed, even after having withdrawn VPA. LEV may benefit myoclonus in PME of mitochondrial origin without altering mitochondrial function, and it could be considered the drug of first choice for the treatment of myoclonus in MERRF.