RESUMEN
Immunophenotyping of inflammatory dermal infiltrates in Malassezia folliculitis (MF) and pityriasis versicolor (PV) lesions is less reported. Immunohistochemistry was performed on 21 MF lesions, 10 PV lesions, and 10 control skin. CD3+, CD4+, CD8+, CD20+, CD68+, and CD117+ cells were increased in MF compared with PV and normal skin (P < 0.01-0.05), while CD3+, CD4+, and CD20+ cells were higher in PV than in normal skin (P < 0.05). Dermal CD1a+ cells were higher only in PV (P < 0.05). Although both cellular and humoral immune responses are involved in pathogenesis of MF and PV, their difference may contribute to clinicopathological discrepancy between two disorders. LAY SUMMARY: Malassezia folliculitis and pityriasis versicolor are common Malassezia-induced superficial mycoses. Their clinicopathological discrepancy may be due to the difference of cellular and humoral immune responses.
Asunto(s)
Dermatomicosis , Foliculitis , Malassezia , Tiña Versicolor , Dermatomicosis/inmunología , Foliculitis/inmunología , Humanos , Inmunofenotipificación , Tiña Versicolor/inmunologíaRESUMEN
BACKGROUND: Atrophying pityriasis versicolor (PV), first described in 1971, is a rare variant in which lesions appear atrophic. OBJECTIVE: We sought to determine the pathophysiology of atrophying PV. METHODS: A retrospective chart review identified 6 cases of atrophying PV. In all cases, routine light microscopy, an elastic tissue stain, and immunohistochemical assessment for the expression of CD3, CD4, CD8, GATA3 and CXCR3 was performed. RESULTS: All cases demonstrated hyperkeratosis with intracorneal infiltration by pathogenic hyphal forms as well as epidermal attenuation and papillary dermal elastolysis. A supervening, mild-to-moderate, superficial lymphocytic infiltrate was noted and characterized by a focal CD8+ T cell-mediated interface dermatitis along with a mixed T-cell infiltrate composed of GATA3+ and CXCR3+ T cells. LIMITATIONS: Small sample size and the loss of some patients to follow-up. CONCLUSION: Atrophying PV represents the sequelae of a mixed helper T-cell (TH1 and TH2) idiosyncratic immune response to Malassezia and can present as a protracted dermatosis that may clinically mimic an atypical lymphocytic infiltrate. TH1 cytokines can recruit histiocytes, a source of elastases, and upregulate matrix metalloproteinase activity, which may contribute to epidermal atrophy.
Asunto(s)
Linfocitos T CD8-positivos/inmunología , Malassezia/inmunología , Células TH1/inmunología , Células Th2/inmunología , Tiña Versicolor/inmunología , Adulto , Anciano , Antígenos CD/análisis , Atrofia , Diagnóstico Diferencial , Femenino , Factor de Transcripción GATA3/análisis , Histiocitos/enzimología , Histiocitos/inmunología , Humanos , Inmunofenotipificación , Lupus Eritematoso Cutáneo/diagnóstico , Malassezia/aislamiento & purificación , Masculino , Persona de Mediana Edad , Micosis Fungoide/diagnóstico , Receptores CXCR3/análisis , Estudios Retrospectivos , Tiña Versicolor/diagnóstico , Tiña Versicolor/patología , Tiña Versicolor/fisiopatologíaRESUMEN
BACKGROUND: Malassezia furfur is a lipophilic yeast that causes skin disease. OBJECTIVE: To evaluate the level of IL-10, IFN-γ and IL-12P70 in co-incubation of peripheral blood mononuclear cells (PBMCs) with M. furfur grown in the presence of some different types of natural oils. METHODS: PBMCs were obtained from blood samples of normal volunteers. M. furfur was cultured in different culture media containing almond oil, fish oil, walnut oil, full-fat milk, and a fat-free medium; and the yeasts grown were harvested and used for co-incubation with PBMCs in vitro. The IFN-γ, IL-10, and IL-12P70 levels were measured at different time intervals using ELISA methods. RESULTS: Generally, IFN-γ and IL-10 levels in the co-incubation of yeasts with walnut oil group (WOG) and fish oil group (FOG) were higher than those in the almond oil group (AOG) and full-fat milk group (FFMG). Although the IL-12P70 was higher in groups such as AOG, FOG, and WOG; the increase was not statistically significant. CONCLUSION: The results demonstrated that the type of fat used by M. furfur in the culture media can influence the immune response and increasesIFN-γ and IL-10 levels in an early time point of the culture system.
Asunto(s)
Leucocitos Mononucleares/efectos de los fármacos , Malassezia/inmunología , Tiña Versicolor/inmunología , Animales , Bovinos , Células Cultivadas , Citocinas/metabolismo , Aceites de Pescado/farmacología , Peces/inmunología , Humanos , Juglans/inmunología , Leucocitos Mononucleares/inmunología , Activación de Linfocitos/efectos de los fármacos , Leche/metabolismo , Aceites de Plantas/farmacologíaRESUMEN
The yeast Malassezia furfur is a part of the resident flora of human skin. It causes various diseases such as pityriasis versicolor, which hardly shows signs of inflammation despite marked clinical symptoms (e.g. hypopigmentation). The pathophysiology related morphological picture might give a clue to this phenomenon. As a part of the literature data are controversial, the present study compared the inflammatory infiltrate of pityriasis versicolor with that of tinea corporis in 40 human skin preparations each from diagnostic specimens. All preparations were stained with HE and PAS. Neutrophilic granulocytes were counted in the HE stain, and hyphae and spores in the PAS stain. The number of counted cells was related to the size of the respective area and the values were compared between pityriasis and tinea corporis. Significantly, more neutrophilic granulocytes were found with tinea corporis (P > 0.01), while they were virtually not demonstrable with pityriasis versicolor. It is surprising that fungal load in the stratum corneum is significantly higher with pityriasis versicolor (P > 0.01). Obviously the immune response involving neutrophilic granulocytes does not occur despite high bacterial load. This might be explained by reduced immunogenicity because of high content of lipids in the cell membrane. Furthermore, pityriarubins that are produced during tryptophan metabolism might be involved, which, in a stimulus-dependent manner, can suppress the ROS production of neutrophilic granulocytes in vivo.
Asunto(s)
Granulocitos/inmunología , Inflamación , Malassezia/patogenicidad , Tiña Versicolor/inmunología , Tiña Versicolor/fisiopatología , Recuento de Colonia Microbiana , Humanos , Inflamación/inmunología , Inflamación/microbiología , Inflamación/fisiopatología , Recuento de Leucocitos , Malassezia/aislamiento & purificación , Esporas Fúngicas/aislamiento & purificación , Tiña Versicolor/microbiología , Triptófano/metabolismoRESUMEN
Malassezia species are members of the human cutaneous commensal flora, in addition to causing a wide range of cutaneous and systemic diseases in suitably predisposed individuals. Studies examining cellular and humoral immune responses specific to Malassezia species in patients with Malassezia-associated diseases and healthy controls have generally been unable to define significant differences in their immune response. The use of varied antigenic preparations and strains from different Malassezia classifications may partly be responsible for this, although these problems can now be overcome by using techniques based on recent work defining some important antigens and also a new taxonomy for the genus. The finding that the genus Malassezia is immunomodulatory is important in understanding its ability to cause disease. Stimulation of the reticuloendothelial system and activation of the complement cascade contrasts with its ability to suppress cytokine release and downregulate phagocytic uptake and killing. The lipid-rich layer around the yeast appears to be pivotal in this alteration of phenotype. Defining the nonspecific immune response to Malassezia species and the way in which the organisms modulate it may well be the key to understanding how Malassezia species can exist as both commensals and pathogens.
Asunto(s)
Dermatomicosis/inmunología , Fungemia/inmunología , Malassezia/inmunología , Malassezia/patogenicidad , Tiña Versicolor/inmunología , Dermatomicosis/microbiología , Fungemia/microbiología , Humanos , Recién Nacido , Malassezia/clasificación , Piel/inmunología , Piel/microbiología , Tiña Versicolor/microbiologíaRESUMEN
BACKGROUND: Malassezia is the aetiological agent of pityriasis versicolor. The mycelial phase of the organism predominates in lesions of pityriasis versicolor. OBJECTIVES: To evaluate the cell-mediated immune (CMI) response to the mycelial phase of Malassezia in patients with this disease, which has not previously been studied. METHODS: The CMI status of 12 patients with pityriasis versicolor and 12 age- and sex-matched controls to mycelial antigen(s) of the organism was examined. The responses to the mycelial form of three strains of the organism were assessed using lymphocyte transformation and leucocyte migration inhibition assays. RESULTS: The transformation responses of the lymphocytes from both patients and controls gave transformation indices < or = 3, although the responses of lymphocytes from patients with pityriasis versicolor to the mycelial form of Malassezia strains were generally higher than those of the controls. In the leucocyte migration inhibition assay, leucocytes from patients with pityriasis versicolor and controls responded to the mycelial antigens of three different Malassezia strains; however, there was no significant difference in leucocyte response between patients with pityriasis versicolor and controls. CONCLUSIONS: Patients with pityriasis versicolor do not therefore have a CMI deficiency to Malassezia mycelial antigens but fail to generate a protective CMI response to mycelial antigens over and above that of control individuals during active disease.
Asunto(s)
Malassezia/inmunología , Tiña Versicolor/inmunología , Adulto , Antígenos Fúngicos/inmunología , Técnicas de Cultivo de Célula , Inhibición de Migración Celular , Femenino , Humanos , Tolerancia Inmunológica , Inmunidad Celular , Activación de Linfocitos/inmunología , Malassezia/clasificación , Malassezia/crecimiento & desarrollo , Masculino , Persona de Mediana EdadRESUMEN
Malassezia yeasts may be a trigger factor for atopic dermatitis. Following the recent reclassification of the genus, the presence of specific IgE antibodies was examined in the sera of patients with atopic dermatitis (n = 223), pityriasis versicolor (n = 83), seborrheic eczema (n = 50) and hymenoptera allergy (n = 39) and in controls without skin diseases (n = 50). In addition to using the commercially available radioallergosorbent test (RAST) for Pityrosporum orbiculare couplings were also made against the reference strains for M. furfur and M. sympodialis. To characterize the specificity and molecular weight of corresponding epitopes identical material was used for production of an immunoblot. Despite high total levels of IgE, controls and patients with pityriasis versicolor showed no specific IgE antibodies. Six patients (12%) with seborrheic eczema were positive while 78 patients (35%) with atopic dermatitis had specific IgE antibodies in higher RAST classes that differed between the Malassezia species. The molecular weights of the main antigens of M. sympodialis and M. furfur were determined to be 15, 22, 30, 37, 40, 58, 79, 92, 99 and 124 kDa and 15, 25, 27, 43, 58, 92, 99 and 107 kDa, respectively. Evaluated according to the location of their disease, patients with head and neck lesions most frequently showed Malassezia-specific IgE antibodies. However, there were differences between the Malassezia species tested, the previously used strain P. orbiculare being assignable to the species M. sympodialis.
Asunto(s)
Anticuerpos Antifúngicos/sangre , Dermatitis Atópica/inmunología , Dermatitis Seborreica/inmunología , Inmunoglobulina E/sangre , Malassezia/inmunología , Tiña Versicolor/inmunología , Adulto , Especificidad de Anticuerpos/inmunología , Dermatitis Atópica/diagnóstico , Dermatitis Seborreica/diagnóstico , Femenino , Humanos , Masculino , Prueba de Radioalergoadsorción , Factores de Riesgo , Especificidad de la Especie , Tiña Versicolor/diagnósticoRESUMEN
The lymphocyte transformation response to Malassezia furfur, Candida albicans, phytohaemagglutinin, concanavlin A and tuberculin purified protein derivative of 12 patients with pityriasis versicolor, 15 patients with seborrhoeic dermatitis and matched controls, was studied. Patients with pityriasis versicolor showed a significantly lower response to M. furfur than patients with seborrhoeic dermatitis and controls.
Asunto(s)
Dermatitis Seborreica/inmunología , Inmunidad Celular/fisiología , Malassezia , Tiña Versicolor/inmunología , Adulto , Candida albicans/inmunología , Concanavalina A/inmunología , Femenino , Humanos , Activación de Linfocitos , Masculino , Persona de Mediana Edad , Fitohemaglutininas/inmunología , Tuberculina/inmunologíaRESUMEN
Humoral immune responses against exoantigen components of oval, elliptic and round yeast forms of Malassezia furfur were analysed by ELISA and Western blotting assays, using sera from patients with pityriasis versicolor (PV), seborrheic dermatitis (SD) and healthy adults (HA), as control. Sera from patients with SD showed IgG anti-oval M. furfur titers ranging from 1/400 to 1/6400 showing geometric mean (GM) of 1/1472, higher than those obtained with sera from patients with PV (1/200 to 1/6400, GM = 1/1239). Both patient groups showed mean titres statistically superior (P < 0.05) than those obtained form HA (GM = 1/229). Similar data were also obtained with the elliptic and round antigens. However, the anti-oval IgG mean titers from patients' sera were much higher than those obtained with elliptic or round antigenic components (p < 0.05) Anti-M furfur IgM titers obtained from patient's sera with PV against all three exoantigens were statistically superior (p < 0.05) than HA group. Patients with SD showed IgM titers statistically superior (p < 0.05) only to oval yeasts of M. furfur. The IgA mean titers from patients' groups against the different morphological antigens were shown be slightly higher than those HA group. By Western blot, using rabbit anti-sera, the different antigenic components of M.furfur showed a close relationship mainly between oval and elliptic yeast cells antigens. The 70 kDa component of the M. furfur exoantigen of oval morphology was recognized by 84% of the PV patients' sera. On the other hand, SD patients' sera recognized 3 principal components of 70 kDa (100%), 65 kDa (67%) and 84 kDa (53%). These components may be considered immunological markers for PV and SD. Twenty-five percent of HA sera recognized the components of 65, 70 and 94 kDa. This investigation shows that M. furfur antigens can sensitize the host, mainly the oval yeast form of M. furfur with a very important specific IgG response in patients with SD and PV.
Asunto(s)
Anticuerpos Antifúngicos/sangre , Dermatitis Seborreica/inmunología , Malassezia/inmunología , Tiña Versicolor/inmunología , Anticuerpos Antifúngicos/inmunología , Especificidad de Anticuerpos , Antígenos Fúngicos/inmunología , Western Blotting , Dermatitis Seborreica/microbiología , Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunoglobulina A/sangre , Inmunoglobulina A/inmunología , Inmunoglobulina G/sangre , Inmunoglobulina G/inmunología , Inmunoglobulina M/sangre , Inmunoglobulina M/inmunología , Malassezia/crecimiento & desarrollo , Tiña Versicolor/microbiologíaRESUMEN
Some measures of systemic host defences, i.e. white cell counts, lymphocyte subsets, delayed-type hypersensitivity and polymorphonuclear leucocyte functions, were evaluated in 42 patients suffering from cutaneous superficial mycoses and in 35 healthy volunteers. Patients were divided according to the extent of their lesions into two groups: group A (30 patients) with skin involvement > or = 30% and group B (12 patients) with skin involvement < or = 1%. No significant abnormalities in systemic defence mechanisms were observed in group B patients, whereas multiple alterations of polymorphonuclear phagocyte activities, i.e. chemotaxis, phagocytosis, mitogen-induced superoxide anion production, were observed in patients with extensive lesions. The values of these phagocytic functions remained significantly reduced (P < 0.01) in these patients after recovery and during a 6-month follow-up period.
Asunto(s)
Dermatomicosis/inmunología , Subgrupos Linfocitarios/inmunología , Neutrófilos/fisiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Candidiasis Cutánea/sangre , Candidiasis Cutánea/inmunología , Quimiotaxis de Leucocito , Dermatomicosis/sangre , Femenino , Humanos , Hipersensibilidad Tardía , Recuento de Leucocitos , Masculino , Persona de Mediana Edad , Fagocitosis , Tiña Versicolor/sangre , Tiña Versicolor/inmunologíaRESUMEN
This study examined the humoral immune responses to Malassezia furfur serovars A, B and C of 10 patients with pityriasis versicolor, 10 patients with seborrheic dermatitis and 20 age- and sex-matched controls. A transferable solid-phase ELISA was used to determine titres of total Igs, IgM, IgA and IgG specific to M. furfur serovars A, B and C. The results demonstrated that patients with seborrheic dermatitis had a significantly higher titre of total Igs to serovar A than patients with pityriasis versicolor; and that patients with seborrheic dermatitis had a significantly higher titre of IgA to serovar C than patients with pityriasis versicolor. The titres of total Igs for controls and patients with seborrheic dermatitis were significantly lower to serovar B than to serovar C. A modified TSP ELISA was used to determine the titres of the IgG subclasses. Titres of IgG1,3,4 to serovar B were significantly higher in seborrheic dermatitis patients than pityriasis versicolor patients and titres of IgG3 to serovar A were significantly higher in seborrheic dermatitis patients than pityriasis versicolor patients. However, despite the differences between the patient groups, none of these results was significantly different to those of controls. Thus, this study did not demonstrate any differences in humoral immunity of patients suffering from Malassezia-associated dermatoses when compared to normal controls. These results may suggest that the humoral immune response to M. furfur is not related to the pathogenesis of Malassezia-associated dermatoses, but simply to the carriage of M. furfur on the skin.
Asunto(s)
Dermatitis Seborreica/inmunología , Malassezia/inmunología , Tiña Versicolor/inmunología , Adulto , Anticuerpos Antifúngicos/análisis , Formación de Anticuerpos , Proteínas del Sistema Complemento/análisis , Humanos , Inmunoglobulina G/clasificación , Inmunoglobulina G/inmunología , Inmunoglobulinas/análisis , Inmunoglobulinas/clasificación , Inmunoglobulinas/inmunología , Persona de Mediana Edad , Valores de ReferenciaRESUMEN
Pityrosporum ovale is a lipophilic yeast that is part of the normal human adult cutaneous flora. It is both a saprophyte and an opportunistic pathogen associated with pityriasis versicolor, Pityrosporum folliculitis, seborrheic dermatitis, and some forms of atopic dermatitis. Systemic infections have also been described. In this article the diagnosis and management of pityriasis versicolor, Pityrosporum folliculitis, seborrheic dermatitis, and atopic dermatitis will be discussed.
Asunto(s)
Dermatitis Atópica , Dermatitis Seborreica , Foliculitis , Tiña Versicolor , Adulto , Antifúngicos/uso terapéutico , Enfermedad Crónica , Dermatitis Atópica/tratamiento farmacológico , Dermatitis Atópica/epidemiología , Dermatitis Atópica/inmunología , Dermatitis Atópica/microbiología , Dermatitis Seborreica/tratamiento farmacológico , Dermatitis Seborreica/epidemiología , Dermatitis Seborreica/inmunología , Dermatitis Seborreica/microbiología , Femenino , Foliculitis/tratamiento farmacológico , Foliculitis/epidemiología , Foliculitis/inmunología , Foliculitis/microbiología , Humanos , Incidencia , Malassezia/aislamiento & purificación , Masculino , Recurrencia , Tiña Versicolor/tratamiento farmacológico , Tiña Versicolor/epidemiología , Tiña Versicolor/inmunología , Tiña Versicolor/microbiologíaRESUMEN
In order to analyse the humoral immune response to the commensal yeast Pityrosporum ovale, we developed a western immunoblot technique with a salt soluble extract of P. ovale cytoplasm. In the present study, we tested sera from patients with psoriasis (n = 15), seborrhoeic dermatitis (n = 10), pityriasis versicolor (n = 8), and normal controls (n = 10). Seventy-three per cent (11/15) of the patients with psoriasis showed specific reactivity with a protein derived from P. ovale of estimated molecular mass 120 kDa, and 46% (7/15) of the cases recognized a 100-kDa protein. Sera from pityriasis versicolor and normal donors showed nonspecific reactivity with several bands of lower molecular weight. To characterize the location of the 100 and 120-kDa proteins, we performed a lyticase digestion of the cell wall, and analysed the soluble digested products by western blotting. The sera from psoriasis patients detected several bands in the range 100-120 kDa. The finding of the immunoreactive 120-kDa protein in this fraction suggests its location at the space between cell wall and membrane (periplasmic space). As a control, we performed an extraction of the cytoplasmic proteins of the dimorphic yeast Candida albicans. C. albicans showed a different pattern of banding in SDS-PAGE. Immunoblots with C. albicans did not allow the detection of any related band. A smear was observed in the high molecular weight range consistent with the presence of lipopolysaccharides. The role of the immune response in infection by P. ovale has not yet been fully explored.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Anticuerpos Antifúngicos/sangre , Proteínas Fúngicas/inmunología , Malassezia/inmunología , Psoriasis/inmunología , Biomarcadores/sangre , Western Blotting , Dermatitis Seborreica/inmunología , Humanos , Psoriasis/microbiología , Tiña Versicolor/inmunologíaRESUMEN
It has been postulated that patients with Malassezia furfur-associated dermatoses have a deficient cell-mediated immune response to M. furfur. This study examined the cell-mediated immune responses to M. furfur serovars A, B and C of 10 patients with pityriasis versicolor and 10 age- and sex-matched controls; and 10 patients with seborrheic dermatitis and 10 age- and sex-matched controls. The responses to each serovar of M. furfur were assessed using the lymphocyte transformation assay and the leukocyte migration inhibition assay. The lymphocyte transformation responses of the patients with pityriasis versicolor to M. furfur serovars A, B and C (0/10, 6/10 and 5/10 respectively) were not significantly different from those of controls (0/10, 2/10 and 1/10). However, for patients with seborrheic dermatitis, significantly more patients' lymphocytes responded to serovars B and C (6/10 and 6/10 respectively) than those of controls (1/10 and 1/10). No patient or control responded to serovar A. In the leukocyte migration inhibition assay, the leukocytes from a greater proportion of patients with pityriasis versicolor (5/7) responded to serovar B than controls (2/10); and the leukocytes from a greater proportion of patients with seborrheic dermatitis (4/10) responded to serovar C than controls (0/9). Thus, this data did not indicate the presence of any cell-mediated immune deficiency to M. furfur in patients with pityriasis versicolor or seborrheic dermatitis, as measured by the lymphocyte transformation assay or the leukocyte migration inhibition assay. The greater responsiveness of T lymphocytes from patients may indicate that T lymphocytes might be involved in the pathogenesis of these diseases.
Asunto(s)
Linfocitos B/inmunología , Dermatitis Seborreica/inmunología , Malassezia/inmunología , Linfocitos T/inmunología , Tiña Versicolor/inmunología , Adulto , Femenino , Humanos , Inmunidad Celular , Subgrupos Linfocitarios/inmunología , Malassezia/clasificación , Masculino , Persona de Mediana Edad , Valores de Referencia , SerotipificaciónRESUMEN
Persistence of chronic tinea pedis (CTP) and pityriasis (tinea) versicolor (PIVE) has been tentatively attributed to an impaired cellular immune response. Therefore immunophenotyping of the inflammatory infiltrates in both disorders was performed in order to detect possible defects in cellular defence. The results of the present study show a dominance of memory T cells, an accumulation of macrophages and lack of B cells. A very prominent feature in CTP and especially PIVE was a marked accumulation of Langerhans cells (LCs) in the epidermis, mostly without expression of CD4. Furthermore, reduced expression of cellular activation markers and the presence of suppressor T cells was noted. In general, the cellular response in PIVE appeared to be slightly stronger than in CTP. This composition of the inflammatory infiltrates in chronic dermatomycoses is similar to previous findings in a variety of dermatoses. However, in PIVE and CTP local reduction of delayed-type hypersensitivity may occur. The function of LCs in these infections is therefore of special interest.
Asunto(s)
Células de Langerhans/inmunología , Tiña del Pie/inmunología , Tiña Versicolor/inmunología , Enfermedad Crónica , Humanos , Piel/inmunología , Linfocitos T Colaboradores-Inductores/inmunologíaRESUMEN
IgE-binding components in crude extracts of Pityrosporum orbiculare were separated by sodium dodecylsulphate polyacrylamide gel electrophoresis and transferred to Immobilon membrane. The components were detected by P. orbiculare radioallergosorbent (RAST) positive sera (class 2-4) from 37 patients with atopic dermatitis and visualized by enzyme-labelled anti-human IgE antibodies in chromogenic substrate. Numerous IgE-binding compounds were demonstrated in the molecular weight (MW) range of 14-94 kD. Eight of them were characterized to molecular size, frequency of reacting sera and intensity of bands. Six of the defined components were found to be major allergens reacting with more than 50% of the sera. They had MWs 86, 76, 67, 28, 17 and 13 kD. Thirty-six of the 37 patients had elevated total serum IgE. Two other patient categories were studied; 11 patients with lymphatic filariasis (high total serum IgE, greater than 1,300 kU/l) and 15 pityriasis versicolor patients (normal total serum IgE, less than 122 kU/l). The P. orbiculare RAST values for these sera showed good accordance with the reactivity in immunoblotting experiments.
Asunto(s)
Alérgenos/análisis , Dermatitis Atópica/inmunología , Inmunoglobulina E/inmunología , Malassezia/inmunología , Adulto , Sitios de Unión de Anticuerpos , Células Cultivadas , Electroforesis en Gel de Poliacrilamida , Filariasis Linfática/inmunología , Humanos , Immunoblotting , Prueba de Radioalergoadsorción , Tiña Versicolor/inmunologíaRESUMEN
By means of RAST investigations, we detected specific IgE antibodies against Pityrosporon orbiculare in the plasma of patients with atopic dermatitis. The patients suffering from the so-called head and neck dermatitis showed an average specific IgE antibody titer of RAST class 3, whereas in those with predominant involvement of the extremities we found an average antibody titer of RAST class 1.