Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
J Endod ; 45(12S): S39-S48, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31623904

RESUMEN

After traumatic injuries to teeth, microorganisms may invade the compromised pulp tissue and initiate pulp infection and periapical inflammation. In addition to bone resorption that typically accompanies pulp necrosis, root resorption frequently occurs. Root resorption has several variants that may occur shortly after the trauma or at a later stage. The pathological changes seen after traumatic injuries to teeth are invariably linked to the presence of microbial irritants. The presence of bacterial biofilms in the dental pulp space can be treated with regenerative or therapeutic endodontic procedures. However, necrosis of periodontal ligament is usually terminal for the tooth involved. In this review, the sources of bacteria after traumatic injuries are discussed. The types and role of microorganisms involved in the pathogenesis of endodontic pathosis after traumatic injuries are presented, and contemporary approaches for the management of these conditions are reviewed. Contemporary antimicrobial strategies are discussed. The rationale for the use of systemic and topical antimicrobials is presented. Finally, novel approaches to the use of antimicrobial therapies, particularly in regenerative procedures, are reviewed.


Asunto(s)
Bacterias , Necrosis de la Pulpa Dental , Resorción Radicular , Traumatismos de los Dientes , Diente , Pulpa Dental , Necrosis de la Pulpa Dental/microbiología , Humanos , Traumatismos de los Dientes/microbiología
2.
Dent Traumatol ; 35(6): 324-332, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31125490

RESUMEN

After traumatic injuries to teeth, microorganisms may invade the compromised pulp tissue and initiate pulp infection and periapical inflammation. In addition to bone resorption that typically accompanies pulp necrosis, root resorption frequently occurs. Root resorption has several variants that may occur shortly after the trauma or at a later stage. The pathological changes seen after traumatic injuries to teeth are invariably linked to the presence of microbial irritants. The presence of bacterial biofilms in the dental pulp space can be treated with regenerative or therapeutic endodontic procedures. However, necrosis of periodontal ligament is usually terminal for the tooth involved. In this review, the sources of bacteria after traumatic injuries are discussed. The types and role of microorganisms involved in the pathogenesis of endodontic pathosis after traumatic injuries are presented, and contemporary approaches for the management of these conditions are reviewed. Contemporary antimicrobial strategies are discussed. The rationale for the use of systemic and topical antimicrobials is presented. Finally, novel approaches to the use of antimicrobial therapies, particularly in regenerative procedures, are reviewed.


Asunto(s)
Necrosis de la Pulpa Dental , Resorción Radicular , Ápice del Diente , Traumatismos de los Dientes , Pulpa Dental , Necrosis de la Pulpa Dental/microbiología , Humanos , Resorción Radicular/microbiología , Diente , Ápice del Diente/microbiología , Traumatismos de los Dientes/microbiología
3.
J Endod ; 40(6): 778-83, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24862703

RESUMEN

INTRODUCTION: Revascularization outcome depends on microbial elimination because apical repair will not happen in the presence of infected tissues. This study evaluated the microbial composition of traumatized immature teeth and assessed their reduction during different stages of the revascularization procedures performed with 2 intracanal medicaments. METHODS: Fifteen patients (7-17 years old) with immature teeth were submitted to the revascularization procedures; they were divided into 2 groups according to the intracanal medicament used: TAP group (n = 7), medicated with a triple antibiotic paste, and CHP group (n = 8), dressed with calcium hydroxide + 2% chlorhexidine gel. Samples were taken before any treatment (S1), after irrigation with 6% NaOCl (S2), after irrigation with 2% chlorhexidine (S3), after intracanal dressing (S4), and after 17% EDTA irrigation (S5). Cultivable bacteria recovered from the 5 stages were counted and identified by means of polymerase chain reaction assay (16S rRNA). RESULTS: Both groups had colony-forming unit counts significantly reduced after S2 (P < .05); however, no significant difference was found between the irrigants (S2 and S3, P = .99). No difference in bacteria counts was found between the intracanal medicaments used (P = .95). The most prevalent bacteria detected were Actinomyces naeslundii (66.67%), followed by Porphyromonas endodontalis, Parvimonas micra, and Fusobacterium nucleatum, which were detected in 33.34% of the root canals. An average of 2.13 species per canal was found, and no statistical correlation was observed between bacterial species and clinical/radiographic features. CONCLUSIONS: The microbial profile of infected immature teeth is similar to that of primarily infected permanent teeth. The greatest bacterial reduction was promoted by the irrigation solutions. The revascularization protocols that used the tested intracanal medicaments were efficient in reducing viable bacteria in necrotic immature teeth.


Asunto(s)
Antibacterianos/uso terapéutico , Antiinfecciosos Locales/uso terapéutico , Apexificación/métodos , Hidróxido de Calcio/uso terapéutico , Clorhexidina/uso terapéutico , Cavidad Pulpar/microbiología , Irrigantes del Conducto Radicular/uso terapéutico , Traumatismos de los Dientes/microbiología , Actinomyces/efectos de los fármacos , Actinomyces/aislamiento & purificación , Adolescente , Carga Bacteriana/efectos de los fármacos , Niño , Ciprofloxacina/uso terapéutico , Cavidad Pulpar/efectos de los fármacos , Necrosis de la Pulpa Dental/microbiología , Necrosis de la Pulpa Dental/terapia , Ácido Edético/uso terapéutico , Fusobacterium nucleatum/efectos de los fármacos , Fusobacterium nucleatum/aislamiento & purificación , Geles , Humanos , Metronidazol/uso terapéutico , Viabilidad Microbiana/efectos de los fármacos , Minociclina/uso terapéutico , Peptostreptococcus/efectos de los fármacos , Peptostreptococcus/aislamiento & purificación , Porphyromonas endodontalis/efectos de los fármacos , Porphyromonas endodontalis/aislamiento & purificación , Hipoclorito de Sodio/uso terapéutico , Ápice del Diente/efectos de los fármacos , Ápice del Diente/microbiología
4.
Dent Traumatol ; 27(6): 438-41, 2011 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-21895973

RESUMEN

Traumatic dental injuries could expose the dentin and, even the pulp, to the oral environment, making possible their contamination. The presence of microorganisms causes pulpal disease and further a tecidual clutter in the periradicular region. The therapy of periradicular pathosis is the consequence of a correct diagnoses which depends on the knowledge of the nature and complexity of endodontic infections. As there is no information on the microbiology of primary endodontic infection in young teeth, the aim of the current study was to investigate the microbiologic status of root canals from permanent young teeth with primary endodontic infection. Twelve patients with the need for endodontic treatment participated in the study. The selected teeth were uniradicular and had an incomplete root formation. They had untreated necrotic pulp. After the access preparation, nineteen microbiologic samples were obtained from the root canals with sterile paper points. Afterwards, the paper points were pooled in a sterile tube containing 2 ml of prereduced transport fluid. The samples were diluted and spread onto plates with selective medium for Enterococcus spp. and for yeast species and onto plates with non-selective medium. A quantitative analysis was performed. The mean number of cultivable bacterial cells in the root canals was 5.7 × 10(6). In four samples (21.05%) black pigmented species were recovered and the mean number of cells was 6.5 × 10(5). One specimen (5.25%) showed the growth of Enterococcus species and the mean number of cells in this case was of 1.5 × 10(4) . The results showed a root canal microbiota with similar design as seen in completely formed teeth.


Asunto(s)
Necrosis de la Pulpa Dental/microbiología , Traumatismos de los Dientes/microbiología , Bacterias Anaerobias/aislamiento & purificación , Carga Bacteriana , Técnicas Bacteriológicas , Bacteroides/aislamiento & purificación , Niño , Cavidad Pulpar/microbiología , Enterococcus/aislamiento & purificación , Humanos , Periodontitis Periapical/microbiología , Preparación del Conducto Radicular/métodos , Ápice del Diente/crecimiento & desarrollo , Adulto Joven
5.
J Dent ; 31(8): 559-68, 2003 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-14554073

RESUMEN

OBJECTIVES: The poor sensitivity of phenotypic identification techniques has hampered the taxonomic differentiation of Actinomyces. Hence we developed a sensitive and specific, PCR-based oligonucleotide-DNA hybridization technique to detect Actinomyces spp. and, used this method to detect these organisms in samples directly obtained from infected root canals. METHODS: A total of 32 samples from 28 Chinese patients, with primary root canal infections, aseptically exposed at the first patient visit, were studied. Whole bacterial genomic DNA was isolated directly from paper point samples. The variable regions of 16S ribosomal DNA of bacteria were amplified and labeled with digoxigenin for further hybridization and detection. A total of seven oligonucleotide probes specific for A. bovis, A. gerencseriae, A. israelii, A. meyeri, catalase-negative A. naeslundii (genospecies 1 and 2), catalase-positive A. naeslundii genospecies 2 and A. odontolyticus were used. RESULTS: 16 of the 32 teeth were infected with one or more Actinomyces species. The prevalence rates of the examined species were: A. odontolyticus 31.3%, A. meyeri 9.4%, A. naeslundii 9.4%, A. israelii 6.3% and A. gerencseriae 3.1%; no A. bovis was detected in any of the canals. Furthermore, A. odontolyticus was isolated more frequently from root canals with caries or a history of caries (Fisher's exact test: P=0.0496; Odds ratio=9.00, 95% confidence interval: 0.97-83.63), and A. naeslundii was significantly associated with traumatized teeth (Fisher's exact test: P=0.0121; Odds ratio=57.00, 95% confidence interval: 2.10-1546.90). However, no significant correlation was found between Actinomyces spp. and clinical symptoms and signs, such as pain, swelling, percussion to tenderness, sinus and periapical radiolucency. CONCLUSION: Actinomyces spp. may be important pathogens of root canal infections. A. naeslundii in particular may be related with traumatized teeth. A. odontolyticus appears to be involved in infections related to caries, exposure of dentinal tubules during cavity preparation and/or leaking restoration, but further clarification with large samples is necessary.


Asunto(s)
Actinomyces/clasificación , Actinomicosis/microbiología , Cavidad Pulpar/microbiología , Enfermedades de la Pulpa Dental/microbiología , Actinomyces/aislamiento & purificación , Adolescente , Adulto , Anciano , China , Intervalos de Confianza , ADN Bacteriano/análisis , Caries Dental/microbiología , Femenino , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Hibridación de Ácido Nucleico , Oportunidad Relativa , Sondas de Oligonucleótidos , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/análisis , Traumatismos de los Dientes/microbiología
6.
Endod Dent Traumatol ; 16(5): 197-204, 2000 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-11202882

RESUMEN

Possible inclusion of contaminant bacteria during surgery has been problematic in studies of periradicular lesions of endodontic origin. Therefore, in this study, two different surgical techniques were compared. A second problem is that some difficult to cultivate species may not be detected using bacteriological methods. Molecular techniques may resolve this problem. DNA-DNA hybridization technology has the additional advantage that DNA is not amplified. The purpose of this investigation was to determine if bacteria from periradicular endodontic lesions could be identified using DNA-DNA hybridization. A full thickness intrasulcular mucoperiosteal (IS) flap (n = 20) or a submarginal (SM) flap (n = 16) was reflected in patients with asymptomatic apical periodontitis. DNA was extracted and incubated with 40 digoxigenin-labeled whole genomic probes. Bacterial DNA was detected in all 36 lesions. Seven probes were negative for all lesions. In patients with sinus tract communication, in teeth lacking intact full coverage crowns, and in patients with a history of trauma 4-13 probes provided positive signals. Seven probes were positive in lesions obtained by the IS, but not the SM technique. Two probes were in samples obtained with the SM technique, but not the IS. Only Bacteroides forsythus and Actinomyces naeslundii genospecies 2 were present in large numbers using either the IS or the SM technique. The SM flap technique, in combination with DNA-DNA hybridization, appeared to provide excellent data pertaining to periradicular bacteria. These results supported other studies that provide evidence of a bacterial presence and persistence in periradicular lesions.


Asunto(s)
Bacterias/clasificación , ADN Bacteriano/genética , Enfermedades de la Pulpa Dental/microbiología , Hibridación de Ácido Nucleico , Enfermedades Periapicales/microbiología , Actinomyces/clasificación , Adulto , Anciano , Bacteroides/clasificación , Sondas de ADN , Fístula Dental/microbiología , Femenino , Genoma Bacteriano , Humanos , Masculino , Persona de Mediana Edad , Periodontitis Periapical/microbiología , Periodontitis Periapical/cirugía , Colgajos Quirúrgicos , Corona del Diente/microbiología , Traumatismos de los Dientes/microbiología
7.
Endod Dent Traumatol ; 12(6): 289-93, 1996 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-9206377

RESUMEN

One of the aims in treating traumatised teeth is to maintain the vitality of the pulp or allow conditions favourable for pulp revascularisation. However, infection of the pulp and root canal system may prevent this. A number of pathways have been proposed that allow bacteria to invade the root canal system, however most of these pathways cannot account for pulp infection in teeth that did not sustain injury to the periodontal attachment. Enamel/dentine cracks have been proposed as a portal for bacterial invasion of seemingly intact teeth and the aim of this study was to determine if bacteria could invade the root canal system after a simulated traumatic episode. Twenty intact and sound upper central incisors were chosen and prepared. One tooth was selected as a sterility control and the external crown surface of the remaining 19 teeth was subjected to infection with Streptococcus gordonii in a bacterial microleakage model. Over 7 days samples of growth media from the root canal system were taken and tested for bacteria. Sixteen of the teeth did not demonstrate bacterial invasion over the time frame. These teeth were then prepared for testing in a pendulum impact device and were subjected to a blow which did not fracture the crowns or dislodge the tooth from its simulated alveolus. The teeth were then prepared and tested in the bacterial microleakage model. After impact seven of the teeth demonstrated bacterial invasion of the root canal system (P = 0.002). These teeth were then reprepared for testing in the bacterial microleakage model. The crowns of five teeth, selected at random, were coated with two layers of light cured unfilled resin, the remaining two were used as positive controls. All the teeth coated with resin did not demonstrate bacterial invasion (P = 0.00), while the positive controls demonstrated invasion. The results suggested that enamel/dentine infractions were pathways for bacterial invasion of the root canal system of traumatised teeth. The application of unfilled resin to the anatomical crown prevented infection.


Asunto(s)
Filtración Dental/etiología , Cavidad Pulpar/microbiología , Traumatismos de los Dientes/complicaciones , Esmalte Dental/lesiones , Filtración Dental/microbiología , Dentina/lesiones , Humanos , Incisivo/lesiones , Streptococcus/aislamiento & purificación , Corona del Diente/lesiones , Fracturas de los Dientes/complicaciones , Fracturas de los Dientes/microbiología , Traumatismos de los Dientes/microbiología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...