Ahorro en los recursos en relación con la utilización del test de ureasa ultrarrápido / Saving resources in relation to ultrafast urease test

No disponible

Validity and cost comparison of 14carbon urea breath test for diagnosis of H Pylori in dyspeptic patients.

AIM: To validate and compare the cost of microdose 14C urea breath test (UBT) with histology and rapid urease test for the diagnosis of H Pylori. METHODS: Ninety-four consecutive patients with dyspeptic symptoms undergoing gastroscopy were enrolled. Gastric biopsies were taken for histology and rapid urease test. UBT was performed after gastroscopy by microdose 14C urea capsules. Sensitivity, specificity and accuracy of UBT were calculated and compared with histology and rapid urease test. Cost comparison of these tests was also performed. RESULTS: H pylori was diagnosed by histology and rapid urease test in 66 (70%) and 61 (65%) patients, while 14C UBT detected infection in 63 (67%). Accuracy of UBT was 93% in comparison with histology while its positive and negative predictive values were 97% and 84%, respectively. Comparison of 14C UBT with rapid urease test gives an accuracy of 96%, with positive and negative predictive values of 95% and 97%, respectively. These results were highly reproducible with a Kappa test (P value<0.001). Cost of histology or rapid urease test with gastroscopy was 110 USD or 95 USD respectively while the cost of UBT was 15 USD. CONCLUSION: Microdose 14C UBT was comparable to histology and rapid urease test. 14C UBT is an economical, self sufficient and suitable test to diagnose active H pylori infection in less developed countries.

Comparaçäo entre testes invasivos para o diagnóstico da infecçäo pelo Helicobacter pylori / Comparison between invasive tests for Helicobacter pylori infection diagnosis

Helicobacter pylori é uma bactéria Gram negativa que coloniza células epiteliais gástricas. Sua presença tem sido associada a várias afecçöes, inclusive gastrite crônica e doença ulcerosa péptica. O diagnóstico da infecçäo pelo Helicobacter pylori pode ser feito através de testes invasivos ou nao-invasivos. Os testes invasivos requerem a realizaçäo de endoscopia digestiva alta para obtençäo de fragmentos de mucosa gástrica que seräo utilizados para a pesquisa da bactéria. Comparam-se quatro testes diagnósticos invasivos: cultura, teste ultra-rápido da urease, histologia (coloraçöes de Hematoxilina-eosina e Giemsa) e esfregaço em lâmina corada com fucsina, com relaçäo à sensibilidade e especificidade, custo e facilidade de execuçäo. O teste da urease mostrou ser o método diagnóstico de mais rápida e fácil execuçäo, com sensibilidade de 86 por cento e especificidade de 100 por cento, apresentando ainda como vantagem o seu baixo custo. Deve, portanto, em nossa opiniäo, ser o método de escolha no diagnóstico do Helicobacter pylori.

Modification of Christensen urease test as an inexpensive tool for detection of Helicobacter pylori.

About half the world population is infected with Helicobacter pylori. Most live in developing countries where clinical studies face the constraints of high costs of imported rapid diagnostic tests. In this work, we describe and validate a simple local urease test (LUT) to determine the presence of the bacterium in gastric biopsies, and report the incidence of infection among symptomatic patients in Caracas, Venezuela. Statistical comparison of LUT and CLOtest (Delta West, Bentley, Australia) (N = 216 patients) showed that the probability of 95% agreement between the two test was 0.936. Overall incidence of infection determined by the LUT was 65% (N = 229), and it was higher in patients from public (72%; N = 153) than from private (50%; N = 76) hospitals (p = .001). Therefore, the incidence of infection differs in two socioeconomic groups that coexist in the same city. LUT may represent an affordable tool in clinical studies needed to identify social factors that increase the risk of infection by H. pylori.

[Comparison between invasive tests for the diagnosis of Helicobacter pylori infections]. / Comparação entre testes invasivos para o diagnóstico da infecção pelo Helicobacter pylori.

Helicobacter pylori is a Gram negative bacteria that colonizes gastric epithelial cells. It has been associated with several gastric disease including chronic gastritis and peptic ulcer. Helicobacter pylori infection diagnosis can be done with invasive and non-invasive methods. In invasive methods an endoscopic gastric mucosa biopsy specimen is used. In our study we compare the sensitivity, specificity, costs and applicability of four invasive diagnostic tests: culture, urease ultra-rapid test, histology (Giemsa and Hematoxilineosin stain) and fuchsin stained mucosal slides. Urease test was the easiest, fastest diagnostic test, with sensitivity of 86% and specificity of 100%, being also the cheapest test. We concluded that it should be the test of choice for Helicobacter pylori infection diagnosis.