RESUMEN
The Tahyna virus (TAHV) is an important human pathogen in the Bunyaviridae family. To date, only the S and M segments of this virus have been sequenced, but the sequence of the L segment hasn't been established yet. In this study, we sequenced 963 nucleotides of the L segment of TAHV, comprising pre-motif A and motif A in region 3 of the RNA polymerase gene.
Asunto(s)
ARN Polimerasas Dirigidas por ADN/genética , Virus de la Encefalitis de California/clasificación , Virus de la Encefalitis de California/genética , Genoma Viral , Filogenia , Proteínas Virales/genética , Secuencias de Aminoácidos , Secuencia de Aminoácidos , ARN Polimerasas Dirigidas por ADN/química , Humanos , Datos de Secuencia Molecular , Orthobunyavirus/clasificación , Orthobunyavirus/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , Proteínas Virales/químicaRESUMEN
We described here the complete nucleotide sequence of the L RNA segment of Oropouche virus (genus Orthobunyavirus, family Bunyaviridae). We found the L RNA segment is 6846 nucleotides long and encodes a putative RNA polymerase of 2250 amino acids. Phylogenetic analysis showed that ORO virus cluster to the Orthobunyavirus genus confirming the serological classification. It also showed that Bunyamwera and California viruses, from the Orthobunyavirus genus, are more closely related to each other than to ORO virus. Sequence comparisons performed between the L proteins of 15 bunyaviruses and the PB1 proteins of 3 influenza viruses revealed that ORO L protein contains the 3 regions characteristic of arenaviruses and bunyaviruses. These comparisons also showed the existence of an additional fourth conserved region in the L protein of bunyaviruses that contains at least two active sites.
Asunto(s)
ARN Polimerasas Dirigidas por ADN/metabolismo , ARN Viral/química , Proteínas Virales/química , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Secuencia de Bases , Secuencia Conservada , ARN Polimerasas Dirigidas por ADN/genética , Virus de la Encefalitis de California/química , Virus de la Encefalitis de California/genética , Genoma Viral , Virus La Crosse/química , Virus La Crosse/genética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Orthomyxoviridae/química , Orthomyxoviridae/genética , Filogenia , ARN Viral/clasificación , ARN Viral/genética , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Proteínas Virales/clasificación , Proteínas Virales/genéticaRESUMEN
In 1983, 17 virus strains were isolated from mosquitoes collected during an outbreak of western equine encephalitis in Santa Fe Province, Argentina. Strains of western equine encephalitis, Venezuelan equine encephalitis, St. Louis encephalitis, and Antequera viruses were isolated, as were several bunyaviruses of the California and Bunyamwera serogroups and a new vesiculovirus. Complement fixation and neutralization tests were used to identify the California serogroup virus as a subtype of Melao virus, the Bunyamwera serogroup virus as a subtype of both Maguari and Playas viruses, and the vesiculovirus as a newly recognized agent for which the name Calchaqui virus is proposed. A limited serosurvey of horses and humans in Santa Fe Province and horses from the adjacent Santiago del Estero Province was performed to determine the prevalence of neutralizing antibody to the subtypes of Melao and Maguari viruses and to Calchaqui virus. The high prevalence of antibodies to these three agents indicates the need for further studies of their disease potential in horses, because they are closely related to several other viruses that are known equine pathogens.