RESUMEN
Apoptosis, a programmed cell death, is characterized by chromatin condensation, numerous vacuoles, reduction in cell volume, and endonuclease cleavage DNA degradation detected in gel electrophoresis as nucleosomal ladder. Here we report that diethylcarbamazine induces DNA fragmentation in microfilariae of Wuchereria bancrofti revealed by ligation-mediated polymerase chain reaction and by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling at the light and electron transmission level.
Asunto(s)
Apoptosis , Dietilcarbamazina/farmacología , Filaricidas/farmacología , Microfilarias/efectos de los fármacos , Wuchereria bancrofti/efectos de los fármacos , Animales , Fragmentación del ADN , ADN de Helmintos/metabolismo , Etiquetado Corte-Fin in Situ , Microfilarias/citología , Microfilarias/ultraestructura , Microscopía , Microscopía Electrónica de Transmisión , Reacción en Cadena de la Polimerasa , Wuchereria bancrofti/citología , Wuchereria bancrofti/ultraestructuraRESUMEN
The fine structure of the sheath and the cuticle of microfilaria of Wuchereria bancrofti was analysed and the results obtained were compared with those from other filarial parasites. Cationized ferritin particles bind to the surface of the sheath and the epicuticle. No binding of colloidal iron hydroxyde particles to the microfilaria surface was observed. Reaction product indicative of carbohydrates containing vic-glycol groups was not observed in thin sections of microfilaria submitted to the periodic acid-thiosemicarbazide-silver proteinate technique. The results obtained are discussed in relation to previous studies using lectins.