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1.
J Food Biochem ; 43(5): e12828, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-31353521

RESUMEN

1-O-alkylglycerols (AKG) are a class of natural ether lipids derived from 1-O-alkyl-2,3-diacyl-sn-glycerols by deacylation. In this study, 1-O-alkylglycerol (AKG) composition was investigated in the hepatopancreas lipids of the crab Paralithodes camtschaticus and the liver lipids of the squid Berryteuthis magister and the skate  Bathyraja parmifera. One of the principal AKG in marine organisms was 1-O-hexadecyl-sn-glycerol (AKG 16:0). To assess AKG influence on melanoma, we evaluated the cytotoxicity and antiproliferative actions of natural AKG 16:0 and synthetic 1-O-octyl-sn-glycerol (AKG 8:0) on three human melanoma cell lines SK-Mel-5, SK-Mel-28, and RPMI-7951. Natural AKG 16:0 in concentration up to 20 µM was not toxic to all cell lines. AKG 8:0 showed no toxicity to cells SK-Mel-5 and SK-Mel-28 in concentrations up to 20 µM but had moderate cytotoxicity to RPMI-7951 cells with an IC50 of 13 µM. Both investigated substances inhibited the proliferation, formation, and growth of cell colonies of RPMI-7951. PRACTICAL APPLICATIONS: AKG exhibit a variety of biological activities, including anticancer effects. In this study, the liver lipids of the skate B. parmifera and the hepatopancreas lipids of crab P. camtschaticus were shown to be sources of AKG. Our data showed that AKG can be used to prevent the formation of new colonies of malignant cells in combination therapy against melanoma. The results will be useful for future studies involving marine ether lipids and the examination of their anticancer properties against malignant cells.


Asunto(s)
Anomuros/química , Decapodiformes/química , Éteres de Glicerilo/farmacología , Melanoma/tratamiento farmacológico , Rajidae , Animales , Éteres de Glicerilo/aislamiento & purificación , Hepatopáncreas/química , Humanos , Inmunoglobulina G/aislamiento & purificación , Inmunoglobulina G/farmacología , Hígado/química , Melfalán/aislamiento & purificación , Melfalán/farmacología
2.
J Asian Nat Prod Res ; 19(7): 691-696, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-27756152

RESUMEN

Chemical investigation on CH2Cl2 extract of the marine sponge Leucandra sp. afforded two new compounds named leucanone A (1) and naamine J (2), together with eight known compounds (3-10). Their structures were elucidated on the basis of NMR spectroscopic analyses, and comparing with the literature. The cytotoxic activities of the compounds were evaluated against four cancer cell lines, and compound 2 showed mild cytotoxic activities against MCF-7, A549, HeLa, and PC9 cancer cell lines with IC50 values in the range of 20.1-45.3 µM.


Asunto(s)
Alcaloides/aislamiento & purificación , Antineoplásicos/aislamiento & purificación , Imidazoles/aislamiento & purificación , Lípidos/aislamiento & purificación , Poríferos/química , Alcaloides/química , Alcaloides/farmacología , Animales , Antineoplásicos/química , Antineoplásicos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Éteres de Glicerilo/química , Éteres de Glicerilo/aislamiento & purificación , Éteres de Glicerilo/farmacología , Células HeLa , Humanos , Imidazoles/química , Imidazoles/farmacología , Concentración 50 Inhibidora , Lípidos/química , Lípidos/farmacología , Células MCF-7 , Biología Marina , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular
3.
Archaea ; 2012: 513231, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23055819

RESUMEN

The relation between archaeal lipid structures and their activity as adjuvants may be defined and explored by synthesizing novel head groups covalently linked to archaeol (2,3-diphytanyl-sn-glycerol). Saturated archaeol, that is suitably stable as a precursor for chemical synthesis, was obtained in high yield from Halobacterium salinarum. Archaeosomes consisting of the various combinations of synthesized lipids, with antigen entrapped, were used to immunize mice and subsequently determine CD8(+) and CD4(+)-T cell immune responses. Addition of 45 mol% of the glycolipids gentiotriosylarchaeol, mannotriosylarchaeol or maltotriosylarchaeol to an archaetidylglycerophosphate-O-methyl archaeosome, significantly enhanced the CD8(+) T cell response to antigen, but diminished the antibody titres in peripheral blood. Archaeosomes consisting of all three triglycosyl archaeols combined with archaetidylglycerophosphate-O-methyl (15/15/15/55 mol%) resulted in approximately additive CD8(+) T cell responses and also an antibody response not significantly different from the archaetidylglycerophosphate-O-methyl alone. Synthetic archaetidylserine played a role to further enhance the CD8(+) T cell response where the optimum content was 20-30 mol%. Vaccines giving best protection against solid tumor growth corresponded to the archaeosome adjuvant composition that gave highest immune activity in immunized mice.


Asunto(s)
Adyuvantes Inmunológicos/farmacología , Vacunas contra el Cáncer/inmunología , Éteres de Glicerilo/farmacología , Halobacterium salinarum/química , Adyuvantes Inmunológicos/administración & dosificación , Adyuvantes Inmunológicos/aislamiento & purificación , Animales , Anticuerpos/sangre , Antígenos de Neoplasias/administración & dosificación , Antígenos de Neoplasias/inmunología , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD8-positivos/inmunología , Vacunas contra el Cáncer/administración & dosificación , Éteres de Glicerilo/administración & dosificación , Éteres de Glicerilo/aislamiento & purificación , Glucolípidos/administración & dosificación , Glucolípidos/aislamiento & purificación , Glucolípidos/farmacología , Ratones , Ratones Endogámicos C57BL , Neoplasias/patología , Neoplasias/prevención & control
4.
J Med Chem ; 55(1): 503-14, 2012 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-22148427

RESUMEN

Extracts of the marine sponge Niphates digitalis collected in Dominica showed strong activity in a cell-based assay designed to detect antagonists of the androgen receptor (AR) that could act as lead compounds for the development of a new class of drugs to treat castration recurrent prostate cancer (CRPC). Assay-guided fractionation showed that niphatenones A (3) and B (4), two new glycerol ether lipids, were the active components of the extracts. The structures of 3 and 4 were elucidated by analysis of NMR and MS data and confimed via total synthesis. Biological evaluation of synthetic analogues of the niphatenones has shown that the enantiomers 7 and 8 are more potent than the natural products in the screening assay and defined preliminary SAR for the new AR antagonist pharmacophore, including the finding that the Michael acceptor enone functionality is not required for activity. Niphatenone B (4) and its enantiomer 8 blocked androgen-induced proliferation of LNCaP prostate cancer cells but had no effect on the proliferation of PC3 prostate cancer cells that do not express functional AR, consistent with activity as AR antagonists. Use of the propargyl ether 44 and Click chemistry showed that niphatenone B binds covalently to the activation function-1 (AF1) region of the AR N-terminus domain (NTD).


Asunto(s)
Antineoplásicos/química , Éteres de Glicerilo/química , Poríferos/química , Neoplasias de la Próstata/genética , Receptores Androgénicos/genética , Animales , Antineoplásicos/síntesis química , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Ensayos de Selección de Medicamentos Antitumorales , Éteres de Glicerilo/síntesis química , Éteres de Glicerilo/aislamiento & purificación , Éteres de Glicerilo/farmacología , Humanos , Espectroscopía de Resonancia Magnética , Masculino , Conformación Molecular , Neoplasias de la Próstata/tratamiento farmacológico , Estereoisomerismo , Relación Estructura-Actividad , Transcripción Genética/efectos de los fármacos
5.
Appl Environ Microbiol ; 77(10): 3468-77, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21441324

RESUMEN

Glycerol dibiphytanyl glycerol tetraether (GDGT)-based intact membrane lipids are increasingly being used as complements to conventional molecular methods in ecological studies of ammonia-oxidizing archaea (AOA) in the marine environment. However, the few studies that have been done on the detailed lipid structures synthesized by AOA in (enrichment) culture are based on species enriched from nonmarine environments, i.e., a hot spring, an aquarium filter, and a sponge. Here we have analyzed core and intact polar lipid (IPL)-GDGTs synthesized by three newly available AOA enriched directly from marine sediments taken from the San Francisco Bay estuary ("Candidatus Nitrosoarchaeum limnia"), and coastal marine sediments from Svalbard, Norway, and South Korea. Like previously screened AOA, the sedimentary AOA all synthesize crenarchaeol (a GDGT containing a cyclohexane moiety and four cyclopentane moieties) as a major core GDGT, thereby supporting the hypothesis that crenarchaeol is a biomarker lipid for AOA. The IPL headgroups synthesized by sedimentary AOA comprised mainly monohexose, dihexose, phosphohexose, and hexose-phosphohexose moieties. The hexose-phosphohexose headgroup bound to crenarchaeol was common to all enrichments and, in fact, the only IPL common to every AOA enrichment analyzed to date. This apparent specificity, in combination with its inferred lability, suggests that it may be the most suitable biomarker lipid to trace living AOA. GDGTs bound to headgroups with a mass of 180 Da of unknown structure appear to be specific to the marine group I.1a AOA: they were synthesized by all three sedimentary AOA and "Candidatus Nitrosopumilus maritimus"; however, they were absent in the group I.1b AOA "Candidatus Nitrososphaera gargensis."


Asunto(s)
Amoníaco/metabolismo , Archaea/química , Archaea/metabolismo , Éteres de Glicerilo/química , Éteres de Glicerilo/aislamiento & purificación , Lípidos de la Membrana/química , Lípidos de la Membrana/aislamiento & purificación , Archaea/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Sedimentos Geológicos/microbiología , Hexosas/análisis , Corea (Geográfico) , Espectrometría de Masas , Oxidación-Reducción , San Francisco , Svalbard
6.
J Nat Prod ; 72(8): 1552-4, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19670881

RESUMEN

Six 1-glyceryl ethers (1-6) were isolated from the red alga-sponge assemblage Ceratodictyon spongiosum/Haliclona cymaeformis. Structural assignments were conducted by interpretation of spectroscopic data and the modified Mosher's method. Four allylic alcohols were obtained as a pair of epimeric mixtures (3/4 and 5/6). These glyceryl ethers exhibited weak cytotoxic activity against HeLa human cervical cancer cells.


Asunto(s)
Éteres de Glicerilo/aislamiento & purificación , Haliclona/química , Rhodophyta/química , Animales , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Éteres de Glicerilo/química , Éteres de Glicerilo/farmacología , Células HeLa , Humanos , Estructura Molecular
7.
Vopr Pitan ; 78(1): 74-8, 2009.
Artículo en Ruso | MEDLINE | ID: mdl-19348288

RESUMEN

On the white Wistar rats with alimentary dyslipidemia investigated influence 1-O-alkyl-glycerides ethers (AGE), received by a method of hydrolysis 1-O-alkyl-diacylglycerides from lipids of the squid Berryteuthis magister liver, on a lipid metabolism, hepatobiliary functions of liver, antioxidant systems and parameters of blood. Are revealed antioxidant, antianemia and immunoactive properties of AGE. AGE raise a level of glucose and activity of enzymes hepatobiliary systems in blood, interfere the decrease of a cholesterol in blood.


Asunto(s)
Decapodiformes/química , Dislipidemias/sangre , Dislipidemias/tratamiento farmacológico , Éteres de Glicerilo/uso terapéutico , Metabolismo de los Lípidos/efectos de los fármacos , Lípidos/química , Hígado/química , Animales , Recuento de Células Sanguíneas , Glucemia/análisis , Colesterol/sangre , Colesterol/metabolismo , Modelos Animales de Enfermedad , Dislipidemias/metabolismo , Éteres de Glicerilo/administración & dosificación , Éteres de Glicerilo/aislamiento & purificación , Peroxidación de Lípido/efectos de los fármacos , Lípidos/aislamiento & purificación , Hígado/metabolismo , Masculino , Ratas , Ratas Wistar
8.
Appl Environ Microbiol ; 74(8): 2433-40, 2008 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-18296531

RESUMEN

In this study we analyzed the membrane lipid composition of "Candidatus Nitrosopumilus maritimus," the only cultivated representative of the cosmopolitan group I crenarchaeota and the only mesophilic isolate of the phylum Crenarchaeota. The core lipids of "Ca. Nitrosopumilus maritimus" consisted of glycerol dialkyl glycerol tetraethers (GDGTs) with zero to four cyclopentyl moieties. Crenarchaeol, a unique GDGT containing a cyclohexyl moiety in addition to four cyclopentyl moieties, was the most abundant GDGT. This confirms unambiguously that crenarchaeol is synthesized by species belonging to the group I.1a crenarchaeota. Intact polar lipid analysis revealed that the GDGTs have hexose, dihexose, and/or phosphohexose head groups. Similar polar lipids were previously found in deeply buried sediments from the Peru margin, suggesting that they were in part synthesized by group I crenarchaeota.


Asunto(s)
Crenarchaeota/química , Éteres de Glicerilo/aislamiento & purificación , Lípidos de la Membrana/análisis , Cromatografía Líquida de Alta Presión , Espectrometría de Masas , Fosfolípidos/análisis
9.
Environ Microbiol ; 10(3): 810-8, 2008 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-18205821

RESUMEN

The widespread occurrence and diversity of ammonia oxidizing Archaea suggests their contribution to the nitrogen cycle is of global significance. Their distribution appeared limited to low- and moderate-temperature environments until the recent finding of a diagnostic membrane lipid, crenarchaeol, in terrestrial hot springs. We report here the cultivation of a thermophilic nitrifier ('Candidatus Nitrosocaldus yellowstonii'), an autotrophic crenarchaeote growing up to 74 degrees C by aerobic ammonia oxidation. The major core lipid of this archaeon growing at 72 degrees C is crenarchaeol, providing the first direct evidence for its synthesis by a thermophile. These findings greatly extend the upper temperature limit of nitrification and document that the capacity for ammonia oxidation is broadly distributed among the Crenarchaeota.


Asunto(s)
Amoníaco/metabolismo , Archaea/metabolismo , Crenarchaeota/metabolismo , Éteres de Glicerilo/metabolismo , Crenarchaeota/genética , Éteres de Glicerilo/aislamiento & purificación , Manantiales de Aguas Termales , Calor , Datos de Secuencia Molecular , Oxidación-Reducción , Temperatura
10.
Appl Environ Microbiol ; 73(19): 6181-91, 2007 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-17693566

RESUMEN

Glycerol dialkyl glycerol tetraethers (GDGTs) are core membrane lipids originally thought to be produced mainly by (hyper)thermophilic archaea. Environmental screening of low-temperature environments showed, however, the abundant presence of structurally diverse GDGTs from both bacterial and archaeal sources. In this study, we examined the occurrences and distribution of GDGTs in hot spring environments in Yellowstone National Park with high temperatures (47 to 83 degrees C) and mostly neutral to alkaline pHs. GDGTs with 0 to 4 cyclopentane moieties were dominant in all samples and are likely derived from both (hyper)thermophilic Crenarchaeota and Euryarchaeota. GDGTs with 4 to 8 cyclopentane moieties, likely derived from the crenarchaeotal order Sulfolobales and the euryarchaeotal order Thermoplasmatales, are usually present in much lower abundance, consistent with the relatively high pH values of the hot springs. The relative abundances of cyclopentane-containing GDGTs did not correlate with in situ temperature and pH, suggesting that other environmental and possibly genetic factors play a role as well. Crenarchaeol, a biomarker thought to be specific for nonthermophilic group I Crenarchaeota, was also found in most hot springs, though in relatively low concentrations, i.e., <5% of total GDGTs. Its abundance did not correlate with temperature, as has been reported previously. Instead, the cooccurrence of relatively abundant nonisoprenoid GDGTs thought to be derived from soil bacteria suggests a predominantly allochthonous source for crenarchaeol in these hot spring environments. Finally, the distribution of bacterial branched GDGTs suggests that they may be derived from the geothermally heated soils surrounding the hot springs.


Asunto(s)
Archaea/química , Bacterias/química , Éteres de Glicerilo/análisis , Manantiales de Aguas Termales/microbiología , Lípidos de la Membrana/análisis , Éteres de Glicerilo/química , Éteres de Glicerilo/aislamiento & purificación , Éteres de Glicerilo/metabolismo , Lípidos de la Membrana/química , Microbiología del Suelo , Estados Unidos
11.
Indoor Air ; 17(4): 305-16, 2007 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-17661927

RESUMEN

UNLABELLED: Ultraviolet photocatalytic oxidation (UVPCO) systems for removal of volatile organic compounds (VOCs) from air are being considered for use in office buildings. Here, we report an experimental evaluation of a UVPCO device with tungsten oxide modified titanium dioxide (TiO2) as the photocatalyst. The device was challenged with complex VOC mixtures. One mixture contained 27 VOCs characteristic of office buildings and another comprised 10 VOCs emitted by cleaning products, in both cases at realistic concentrations (low ppb range). VOC conversion efficiencies varied widely, usually exceeded 20%, and were as high as approximately 80% at about 0.03 s residence time. Conversion efficiency generally diminished with increased airflow rate, and followed the order: alcohols and glycol ethers > aldehydes, ketones, and terpene hydrocarbons > aromatic and alkane hydrocarbons > halogenated aliphatic hydrocarbons. Conversion efficiencies correlated with the Henry's law constant more closely than with other physicochemical parameters. An empirical model based on the Henry's law constant and the gas-phase reaction rate with hydroxyl radical provided reasonable estimates of pseudo-first order photocatalytic reaction rates. Formaldehyde, acetaldehyde, acetone, formic acid and acetic acid were produced by the device due to incomplete mineralization of common VOCs. Formaldehyde outlet/inlet concentration ratios were in the range 1.9-7.2. PRACTICAL IMPLICATIONS: Implementation of air cleaning technologies for both VOCs and particles in office buildings may improve indoor air quality, or enable indoor air quality levels to be maintained with reduced outdoor air supply and concomitant energy savings. One promising air cleaning technology is ultraviolet photocatalytic oxidation (UVPCO) air cleaning. For the prototype device evaluated here with realistic mixtures of VOCs, conversion efficiencies typically exceeded the minimum required to counteract predicted VOC concentration increases from a 50% reduction in ventilation. However, the device resulted in the net generation of formaldehyde and acetaldehyde from the partial oxidation of ubiquitous VOCs. Further development of the technology is needed to eliminate these hazardous air pollutants before such a UVPCO device can be deployed in buildings.


Asunto(s)
Contaminantes Atmosféricos/efectos de la radiación , Contaminación del Aire Interior/prevención & control , Filtración/métodos , Compuestos Orgánicos/aislamiento & purificación , Rayos Ultravioleta , Contaminantes Atmosféricos/química , Alcoholes/aislamiento & purificación , Aldehídos/aislamiento & purificación , Catálisis , Filtración/instrumentación , Éteres de Glicerilo/aislamiento & purificación , Hidrocarburos/aislamiento & purificación , Cetonas/aislamiento & purificación , Cinética , Oxidación-Reducción , Fotoquímica , Titanio/química , Volatilización
12.
Zhong Yao Cai ; 29(6): 555-7, 2006 Jun.
Artículo en Chino | MEDLINE | ID: mdl-17039875

RESUMEN

Nine compounds, cholesterol (1), ergostra-7,22-diene-3beta, 5alpha, 6beta-triol (2), cholesta-7,22-diene-3beta, 5alpha, 6beta-triol (3),5,8-epidioxycampesta-6,22-dien-3-o1 (4), batyl alcohol (5), theine (6), thymine (7), uracil (8), guanine (9), were isolated from the South China Sea gorgonian coral Subergorgia reticulata and their structures elucidated on the basis of spectral data. All of these compounds were isolated for the first time from this gorgonian coral.


Asunto(s)
Antozoos/química , Colesterol/aislamiento & purificación , Éteres de Glicerilo/aislamiento & purificación , Timina/aislamiento & purificación , Animales , Antozoos/clasificación , Colesterol/química , Éteres de Glicerilo/química , Guanina/química , Guanina/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Timina/química , Uracilo/química , Uracilo/aislamiento & purificación
13.
Arch Pharm Res ; 28(3): 290-3, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15832815

RESUMEN

A known monoalkyl glycerol ether, (+/-)-1-nonadecyloxy-2,3-propanediol (1) was isolated from the ethyl acetate extract of a soft coral Dendronephthya gigantea as a weakly cytotoxic constituent against four human cancer cell lines, A549, HT-29, HT-1080, and SNU-638. In addition, a known ceramide, (2S,3R,4E,8E)-N-hexadecanoyl-2-amino-4,8-octadecadiene-1,3-diol (2), was also isolated as an inactive constituent. This is the first report on the isolation of the compounds 1 and 2 from the octocoral, Dendronephthya species.


Asunto(s)
Alcadienos/aislamiento & purificación , Antozoos/química , Antineoplásicos/aislamiento & purificación , Ceramidas/aislamiento & purificación , Éteres de Glicerilo/aislamiento & purificación , Alcadienos/química , Animales , Antineoplásicos/química , Antineoplásicos/farmacología , Línea Celular Tumoral , Ceramidas/química , Ensayos de Selección de Medicamentos Antitumorales , Éteres de Glicerilo/química , Éteres de Glicerilo/farmacología , Humanos
15.
Appl Environ Microbiol ; 70(9): 5229-37, 2004 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-15345404

RESUMEN

Glycerol dialkyl glycerol tetraethers (GDGTs) are core membrane lipids of the Crenarchaeota. The structurally unusual GDGT crenarchaeol has been proposed as a taxonomically specific biomarker for the marine planktonic group I archaea. It is found ubiquitously in the marine water column and in sediments. In this work, samples of microbial community biomass were obtained from several alkaline and neutral-pH hot springs in Nevada, United States. Lipid extracts of these samples were analyzed by high-performance liquid chromatography-mass spectrometry and by gas chromatography-mass spectrometry. Each sample contained GDGTs, and among these compounds was crenarchaeol. The distribution of archaeal lipids in Nevada hot springs did not appear to correlate with temperature, as has been observed in the marine environment. Instead, a significant correlation with the concentration of bicarbonate was observed. Archaeal DNA was analyzed by denaturing gradient gel electrophoresis. All samples contained 16S rRNA gene sequences which were more strongly related to thermophilic crenarchaeota than to Cenarchaeum symbiosum, a marine nonthermophilic crenarchaeon. The occurrence of crenarchaeol in environments containing sequences affiliated with thermophilic crenarchaeota suggests a wide phenotypic distribution of this compound. The results also indicate that crenarchaeol can no longer be considered an exclusive biomarker for marine species.


Asunto(s)
Éteres de Glicerilo/aislamiento & purificación , Manantiales de Aguas Termales/microbiología , Archaea/aislamiento & purificación , Cromatografía de Gases y Espectrometría de Masas , Geografía , Lípidos de la Membrana/análisis , Datos de Secuencia Molecular , Nevada
17.
Zhong Yao Cai ; 24(9): 640-1, 2001 Sep.
Artículo en Chino | MEDLINE | ID: mdl-11799771

RESUMEN

Three compounds, N-1-(hydroxymethyl)-2-hydroxyl-(E,E)-3,7-heptadecadienal-hexadecanamide(1), batyl alcohol(2) and n-cetanol(3) were isolated from the EtOAc portion of the ethanol extract of the hard coral Acropora pulchra collected from north China sea, and their structures were determined by MS, 1HNMR, 13CNMR analysis.


Asunto(s)
Cnidarios/química , Alcoholes Grasos/química , Éteres de Glicerilo/química , Ácidos Palmíticos/química , Animales , Alcoholes Grasos/aislamiento & purificación , Éteres de Glicerilo/aislamiento & purificación , Ácidos Palmíticos/aislamiento & purificación
18.
Lipids ; 35(10): 1155-7, 2000 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-11104022

RESUMEN

The structures of three kinds of phospholipids (PL-X, PL-Y, and PL-T) isolated from Thermoplasma acidophilum have been characterized. The core lipid of PL-Y was caldarchaeol, and that of PL-X was archaeol. The composition of the hydrocarbon chains of the PL-T core lipid was C20 phytane and C40 isoprenoid in a molar ratio of 2 to 1. The major molecular species of the C40 isoprenoid was acyclic without the cyclopentane ring. These three kinds of intact phospholipids commonly had glycerophosphate residues as polar head groups. The structure of PL-T was characterized as trialkyl-type caldarchaetidylglycerol, PL-Y as caldarchaetidylglycerol, and PL-X as archaetidylglycerol.


Asunto(s)
Fosfolípidos/química , Fosfolípidos/aislamiento & purificación , Thermoplasma/química , Cromatografía , Cromatografía en Capa Delgada , Éteres de Glicerilo/química , Éteres de Glicerilo/aislamiento & purificación , Modelos Químicos , Espectrometría de Masa Bombardeada por Átomos Veloces
19.
Rapid Commun Mass Spectrom ; 14(7): 585-9, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-10775092

RESUMEN

A method combining normal phase high performance liquid chromatography (HPLC) with positive ion atmospheric pressure chemical ionization mass spectrometry (APCI-MS) was developed for the analysis of intact glycerol dialkyl glycerol tetraethers (GDGTs) in archaeal cell material and sediments. All GDGTs previously reported to occur in the thermophilic archaeon Sulfolobus solfataricus could be identified based on their mass spectra and retention time. Positive ion mass spectra consisted of abundant protonated molecules and fragment ions corresponding to loss of water and the glycerol moiety. In addition, two novel GDGTs representing alternative combinations of biphytanyl moieties were observed. Using this method, the tetraethers present in the thermophilic archaeon Metallosphaera sedula and two sediment samples were characterized. This rapid method will greatly contribute to the establishment of the sedimentary record of these compounds and increase our understanding of archaea and their occurrence in widely different environments.


Asunto(s)
Archaea/química , Cromatografía Líquida de Alta Presión , Sedimentos Geológicos/análisis , Éteres de Glicerilo/análisis , Espectrometría de Masas/métodos , Éteres de Glicerilo/aislamiento & purificación , Espectrometría de Masas/instrumentación , Modelos Moleculares , Ácido Fitánico/análisis , Ácido Fitánico/aislamiento & purificación , Especificidad de la Especie , Sulfolobus/química
20.
Zhong Yao Cai ; 22(2): 75-7, 1999 Feb.
Artículo en Chino | MEDLINE | ID: mdl-12575047

RESUMEN

The studies on the chemical constituents of soft coral Sarcophyton elegans led to the isolation of gorgosterol, batyl alcohol and simumeramide. Their structures were identified on the comprehensive spectral analysis. These compounds were first obtained from the soft coral.


Asunto(s)
Antozoos/química , Colesterol/análogos & derivados , Colesterol/aislamiento & purificación , Ácidos Grasos/aislamiento & purificación , Éteres de Glicerilo/aislamiento & purificación , Materia Medica/química , Animales , Colesterol/química , Ácidos Grasos/química , Éteres de Glicerilo/química
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