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1.
Washington; Organización Panamericana de la Salud; abr. 13, 2020.
No convencional en Español | Coleciona SUS | ID: biblio-1096694

RESUMEN

Ante la situación actual de la pandemia del COVID-19 se aconseja a los países que continúen con la adopción de los algoritmos de diagnóstico de TB recomendados por OPS/OMS. A pesar de las diferencias en los modos de transmisión de TB y COVID-19, ciertas medidas de protección personal son relevantes para ambas enfermedades. Las medidas habituales para protegerse de la TB deben continuar junto con las precauciones adicionales para proteger a los trabajadores de COVID-19.


Asunto(s)
Neumonía Viral/prevención & control , Tuberculosis/diagnóstico , Tuberculosis/prevención & control , ADN Viral/análisis , Infecciones por Coronavirus/prevención & control , Pandemias/prevención & control , Betacoronavirus
2.
Zhonghua Zhong Liu Za Zhi ; 42(3): 252-256, 2020 Mar 23.
Artículo en Chino | MEDLINE | ID: mdl-32252206

RESUMEN

Objective: To evaluate the performance of Hybribio human papillomavirus (HPV) typing test kit for high risk HPV-DNA typing detection in screening of cervical precancer lesions. Methods: A total of 9 914 women were recruited in Henan, Shanxi, and Guangdong provinces from June to July 2017. All women underwent HPV DNA test. The women who diagnosed as HPV positive and cytological examination ≥ atypical squamous cells of undetermined significance (ASCUS) or HPV negative and cytological examination≥low-grade squamous intraepithelial lesions (LSIL) underwent colposcopy biopsy and pathological examination. Using the pathological diagnosis as the gold standard, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and 95% confidence interval (CI) of high-risk HPV and HPV16/18 tests were calculated. Results: The mean age of 9 914 subjects was (45.0±9.3) years old. Among them, 1 302 subjects were detected as high risk HPV positive, including 211 of HPV16 positive and 64 of HPV18 positive. According to the pathological gold standard of cervical intraepithelial neoplasia grade 2 (CIN2) or worse, the sensitivity and specificity of high risk-HPV and HPV 16/18 for triaging ASCUS women were 90.6% (95%CI: 75.8%-96.8%) and 78.0% (95%CI: 74.5%-81.2%) as well as 56.3% (95%CI: 39.3%-71.8%) and 95.7% (95%CI: 93.8%-97.1%), respectively. The sensitivity and specificity of high risk-HPV and HPV 16/18 for cervical precancer lesions screening were 95.1% (95%CI: 88.1%-98.1%) and 87.6% (95%CI: 86.9%-88.2%) as well as 65.9% (95%CI: 55.1%-75.2%) and 97.8% (95%CI: 97.5%-98.1%), respectively. Conclusions: The Hybribio HPV test kit has a relative high sensitivity and specificity for cervical precancer lesions screening and ASCUS triaging. It is reliable for HPV DNA detection and cervical cancer screening.


Asunto(s)
Neoplasia Intraepitelial Cervical/diagnóstico , Detección Precoz del Cáncer , Papillomavirus Humano 16/genética , Papillomavirus Humano 18/genética , Prueba de Papanicolaou , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/complicaciones , Neoplasias del Cuello Uterino/diagnóstico , Adulto , Biopsia , Neoplasia Intraepitelial Cervical/patología , Neoplasia Intraepitelial Cervical/virología , Cuello del Útero/patología , Cuello del Útero/virología , Colposcopía , ADN Viral/análisis , Femenino , Genotipo , Humanos , Persona de Mediana Edad , Papillomaviridae/clasificación , Papillomaviridae/genética , Infecciones por Papillomavirus/diagnóstico , Infecciones por Papillomavirus/virología , Valor Predictivo de las Pruebas , Embarazo , Sensibilidad y Especificidad , Neoplasias del Cuello Uterino/patología , Neoplasias del Cuello Uterino/virología
3.
Clin Chim Acta ; 505: 172-175, 2020 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-32156607

RESUMEN

BACKGROUND: There's an outbreak of a novel coronavirus (SARS-CoV-2) infection since December 2019, first in China, and currently with more than 80 thousand confirmed infection globally in 29 countries till March 2, 2020. Identification, isolation and caring for patients early are essential to limit human-to-human transmission including reducing secondary infections among close contacts and health care workers, preventing transmission amplification events. The RT-PCR detection of viral nucleic acid test (NAT) was one of the most quickly established laboratory diagnosis method in a novel viral pandemic, just as in this COVID-19 outbreak. METHODS: 4880 cases that had respiratory infection symptoms or close contact with COVID-19 patients in hospital in Wuhan, China, were tested for SARS-CoV-2 infection by use of quantitative RT-PCR (qRT-PCR) on samples from the respiratory tract. Positive rates were calculated in groups divided by genders or ages. RESULTS: The positive rate was about 38% for the total 4880 specimens. Male and older population had a significant higher positive rates. However, 57% was positive among the specimens from the Fever Clinics. Binary logistic regression analysis showed that age, not gender, was the risk factor for SARS-CoV-2 infection in fever clinics. CONCLUSIONS: Therefore, we concluded that viral NAT played an important role in identifying SARS-CoV-2 infection.


Asunto(s)
Betacoronavirus/química , Infecciones por Coronavirus/diagnóstico , ADN Viral/análisis , Neumonía Viral/diagnóstico , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Adolescente , Adulto , Factores de Edad , Anciano , Líquido del Lavado Bronquioalveolar/virología , China/epidemiología , Técnicas de Laboratorio Clínico , Infecciones por Coronavirus/epidemiología , Brotes de Enfermedades , Femenino , Humanos , Masculino , Persona de Mediana Edad , Nucleocápside/química , Nucleocápside/genética , Pandemias , Neumonía Viral/epidemiología , Sistema Respiratorio/virología , Factores de Riesgo , Factores Sexuales , Esputo/virología , Adulto Joven
4.
Arch Virol ; 165(5): 1099-1107, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32152788

RESUMEN

To determine the status of human cytomegalovirus (HCMV) infection in human milk in China, a total of 510 human milk samples obtained from three provinces, including 211 donor human milk samples from human milk banks and 299 milk samples obtained from the mothers of premature infants, were tested to detect HCMV DNA. Overall, 46.4% of the donated milk samples and 59.2% of the samples obtained from mothers of premature infants were positive for HCMV DNA. The concentration of HCMV DNA was approximately 103 -104 copies/ml in the HCMV-DNA-positive human milk samples. Based on the nucleotide sequence of a 299- to 305-bp fragment of the glycoprotein B (gB) gene, three HCMV genotypes (gB1, gB2 and gB3) were identified in human milk samples. Mixed infection with genotypes gB1 and gB3 was also found in four milk samples from mothers. Genotype gB1 was the predominant genotype in the HCMV-DNA-positive human milk samples, and it could be subdivided into three lineages. There were also some characteristic nucleotides and amino acids in the three HCMV genotypes, which were helpful for distinguishing the genotypes. This is the first study to clarify the HCMV infection status and genetic characteristics of human milk obtained from banks in China, which will be helpful in preventing postnatal HCMV infections and ensuring the safety of human milk banks.


Asunto(s)
Citomegalovirus/clasificación , Citomegalovirus/aislamiento & purificación , Genotipo , Bancos de Leche , Leche Humana/virología , China , Citomegalovirus/genética , ADN Viral/análisis , ADN Viral/genética , Humanos , Prevalencia , Análisis de Secuencia de ADN , Proteínas del Envoltorio Viral/genética
5.
Zhonghua Gan Zang Bing Za Zhi ; 28(2): 179-182, 2020 Feb 20.
Artículo en Chino | MEDLINE | ID: mdl-32164074

RESUMEN

Small interfering RNA (siRNA) is mainly involved in RNA interference for stopping gene translation by targeting and degrading HBV-transcribed mRNA. Targeting and stability in siRNA can be enhanced via chemical modification, combination use and improved delivery system. Clinical studies have identified JNJ-3989 (ARO-HBV) and ARB-1740 as well-tolerated siRNA drugs, which significantly reduce HBsAg levels. This article expounds the main mechanisms of siRNA in inhibiting HBsAg expression, improving target and stability as well as relevant preclinical and clinical studies.


Asunto(s)
Virus de la Hepatitis B/efectos de los fármacos , ARN Interferente Pequeño/farmacología , Replicación Viral/efectos de los fármacos , ADN Viral/análisis , Antígenos de Superficie de la Hepatitis B/análisis , Virus de la Hepatitis B/genética , Hepatitis B Crónica , Humanos , Interferencia de ARN
6.
Invest Ophthalmol Vis Sci ; 61(2): 47, 2020 02 07.
Artículo en Inglés | MEDLINE | ID: mdl-32106294

RESUMEN

Purpose: Microbial ecosystems interact with the human body and affect human health. The microbial community on the ocular surface remains an underexplored territory despite its importance as the first line of defense barrier that protects the eye and ultimately sight. We investigated how age and sex affected human ocular surface microbiome, and in the present study wanted to understand how geographic difference shaped the microbiome in the ocular surface. Methods: We collected conjunctival specimens of 172 eyes from 86 healthy volunteers living in three Chinese cities, namely, Guangzhou, Wenzhou, and Beijing. Using the direct metagenomic shotgun sequencing approach, we characterized how geographic difference affected the human ocular microbiome. Results: We surveyed the taxonomic composition and metabolic function of the microbiota on human ocular surface. We showed that the ocular surface microbiota was composed of bacteria, viruses, and fungi. A geographical difference in both composition and function of the conjunctival microbiome suggests that the environment people lived in shapes their conjunctival microbiome, especially the dominate species. Conclusions: Our study provides a reference catalog of the healthy conjunctival metagenome and raises a concern for environmental influences on the ocular surface microbiome.


Asunto(s)
Conjuntiva/microbiología , Microbiota , Factores de Edad , China , ADN Bacteriano/análisis , ADN de Hongos/análisis , ADN Viral/análisis , Femenino , Humanos , Masculino , Metagenoma , Factores Sexuales
7.
PLoS One ; 15(2): e0229415, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32109945

RESUMEN

Avian adenoviruses (AdVs) are a very diverse group of pathogens causing diseases in poultry and wild birds. Wild birds, endangered by habitat loss and habitat fragmentation in the tropical forests, are recognised to play a role in the transmission of various AdVs. In this study, two novel, hitherto unknown AdVs were described from faecal samples of smooth-billed ani and tropical screech owl. The former was classified into genus Aviadenovirus, the latter into genus Atadenovirus, and both viruses most probably represent new AdV species as well. These results show that there is very limited information about the biodiversity of AdVs in tropical wild birds, though viruses might have a major effect on the population of their hosts or endanger even domesticated animals. Surveys like this provide new insights into the diversity, evolution, host variety, and distribution of avian AdVs.


Asunto(s)
Infecciones por Adenoviridae/veterinaria , Adenoviridae/genética , Adenoviridae/aislamiento & purificación , Aves/virología , ADN Viral/análisis , Estrigiformes/virología , Adenoviridae/clasificación , Infecciones por Adenoviridae/virología , Animales , Aves/genética , ADN Viral/genética , Filogenia , Estrigiformes/genética
8.
Lancet ; 395(10227): 888-898, 2020 03 14.
Artículo en Inglés | MEDLINE | ID: mdl-32085823

RESUMEN

BACKGROUND: Antiretroviral therapy (ART) cannot cure HIV infection because of a persistent reservoir of latently infected cells. Approaches that force HIV transcription from these cells, making them susceptible to killing-termed kick and kill regimens-have been explored as a strategy towards an HIV cure. RIVER is the first randomised trial to determine the effect of ART-only versus ART plus kick and kill on markers of the HIV reservoir. METHODS: This phase 2, open-label, multicentre, randomised, controlled trial was undertaken at six clinical sites in the UK. Patients aged 18-60 years who were confirmed as HIV-positive within a maximum of the past 6 months and started ART within 1 month from confirmed diagnosis were randomly assigned by a computer generated randomisation list to receive ART-only (control) or ART plus the histone deacetylase inhibitor vorinostat (the kick) and replication-deficient viral vector T-cell inducing vaccines encoding conserved HIV sequences ChAdV63. HIVconsv-prime and MVA.HIVconsv-boost (the kill; ART + V + V; intervention). The primary endpoint was total HIV DNA isolated from peripheral blood CD4+ T-cells at weeks 16 and 18 after randomisation. Analysis was by intention to treat. This trial is registered with ClinicalTrials.gov, NCT02336074. FINDINGS: Between June 14, 2015 and Jul 11, 2017, 60 men with HIV were randomly assigned to receive either an ART-only (n=30) or an ART + V + V (n=30) regimen; all 60 participants completed the study, with no loss-to-follow-up. Mean total HIV DNA at weeks 16 and 18 after randomisation was 3·02 log10 copies HIV DNA per 106 CD4+ T-cells in the ART-only group versus 3·06 log10 copies HIV DNA per 106 CD4+ T-cells in ART + V + V group, with no statistically significant difference between the two groups (mean difference of 0·04 log10 copies HIV DNA per 106 CD4+ T-cells [95% CI -0·03 to 0·11; p=0·26]). There were no intervention-related serious adverse events. INTERPRETATION: This kick and kill approach conferred no significant benefit compared with ART alone on measures of the HIV reservoir. Although this does not disprove the efficacy kick and kill strategy, for future trials enhancement of both kick and kill agents will be required. FUNDING: Medical Research Council (MR/L00528X/1).


Asunto(s)
Vacunas contra el SIDA/administración & dosificación , Antirretrovirales/uso terapéutico , Reservorios de Enfermedades , Infecciones por VIH , Inhibidores de Histona Desacetilasas/administración & dosificación , Vorinostat/administración & dosificación , Adulto , ADN Viral/análisis , Infecciones por VIH/tratamiento farmacológico , Humanos , Masculino , Transcripción Genética/efectos de los fármacos , Resultado del Tratamiento
9.
Euro Surveill ; 25(3)2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31992392

RESUMEN

Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.


Asunto(s)
ADN Viral/análisis , Ebolavirus/genética , Ebolavirus/aislamiento & purificación , Genoma Viral/genética , Fiebre Hemorrágica Ebola/diagnóstico , Reacción en Cadena de la Polimerasa/métodos , ARN Viral/genética , Secuencia de Bases , Enfermedades Transmisibles Emergentes , Ebolavirus/clasificación , Humanos , Sensibilidad y Especificidad , Análisis de Secuencia de ADN
11.
BMC Infect Dis ; 20(1): 38, 2020 Jan 14.
Artículo en Inglés | MEDLINE | ID: mdl-31937247

RESUMEN

BACKGROUND: Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis in newborns. The CDC therefore recommends GBS screening for all pregnant women at 35-37 weeks of gestation and administration of intrapartum prophylaxis (in those that tested positive) as an effective means of controlling disease transmission. Several FDA approved molecular diagnostic tests are available for rapid and accurate detection of GBS in antepartum women. METHOD: In this study, we report a clinical comparison of the Xpert GBS LB assay and a novel FDA-cleared test, Revogene GBS LB assay. A total of 250 vaginal-rectal swabs from women undergoing prenatal screening were submitted to the University of Wisconsin's clinical microbiology laboratory for GBS testing. RESULTS: We found 96.8% of samples were concordant between the two tests, while 3.2% were discordant with a positive percent agreement of 98.0% and a negative percent agreement of 96.5% between the Revogene GBS LB assay and the GeneXpert GBS LB assay. CONCLUSION: Overall, we report that both assays perform well for the detection of GBS colonization in pregnant women.


Asunto(s)
Pruebas Diagnósticas de Rutina/métodos , Tamizaje Masivo/métodos , Técnicas de Diagnóstico Molecular/métodos , Complicaciones Infecciosas del Embarazo/diagnóstico , Diagnóstico Prenatal/métodos , Infecciones Estreptocócicas/diagnóstico , Streptococcus agalactiae/genética , ADN Viral/análisis , Femenino , Técnicas Genéticas , Humanos , Transmisión Vertical de Enfermedad Infecciosa/prevención & control , Tamizaje Masivo/economía , Técnicas de Diagnóstico Molecular/economía , Embarazo , Complicaciones Infecciosas del Embarazo/virología , Mujeres Embarazadas , Infecciones Estreptocócicas/virología , Factores de Tiempo , Vagina/virología
12.
Wien Klin Wochenschr ; 132(1-2): 35-41, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31820101

RESUMEN

BACKGROUND: Rapid identification of human herpesviruses from lesion swabs is necessary for timely initiation of antiviral treatment, especially with infections involving neonates and immunocompromised individuals. The aim of the study was to investigate the results of an in-house polymerase chain reaction (PCR) test for herpesviruses in patients with symptoms suggestive for a herpesvirus infection. PATIENTS AND METHODS: In this single center retrospective study the results of 3677 lesion swab specimens tested for human herpes simplex virus 1 and 2 (HSV 1 and 2) and varicella zoster virus (VZV) were analyzed in the context of data sheets giving details of the suspected diagnosis, medical history as well as the demographic data of the patients. The PCR procedures for cytomegalovirus (CMV), Epstein-Barr virus (EBV) and human herpes virus 8 (HHV-8) were applied on special occasions. RESULTS: Of the samples 3369 (91.6%) were swabs and a minority were tissue or blood samples. Of the 3015 samples tested for HSV­1, HSV­2 and VZV concomitantly, 52.3% were positive for at least one of these viruses. Clinically distinct conditions, such as herpes zoster and varicella had a high rate of positive PCR results, ranging from 81% to 88%, respectively. Among HSV­2 positive samples, 23.7% derived from human immunodeficiency virus (HIV) positive patients, in contrast to the 10.8% originating from immunocompetent patients, the difference being statistically significant (p < 0.002). The HSV­2 was detected more often in women than in men. CONCLUSION: Distinct clinical diagnoses have a high correlation rate with positive PCR results. A significantly higher number of HSV­2 positive results were found in HIV positive patients and in women.


Asunto(s)
Dermatología , Infecciones por Herpesviridae , Reacción en Cadena de la Polimerasa , ADN Viral/análisis , Dermatología/tendencias , Femenino , Infecciones por Herpesviridae/diagnóstico , Infecciones por Herpesviridae/genética , Humanos , Recién Nacido , Masculino , Estudios Retrospectivos
13.
Genes Chromosomes Cancer ; 59(2): 84-95, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-31407403

RESUMEN

Cervical carcinogenesis, the second leading cause of cancer death in women worldwide, is caused by multiple types of human papillomaviruses (HPVs). To investigate a possible role for HPV in a cervical carcinoma that was HPV-negative by PCR testing, we performed HPV DNA hybridization capture plus massively parallel sequencing. This detected a subgenomic, URR-E6-E7-E1 segment of HPV70 DNA, a type not generally associated with cervical cancer, inserted in an intron of the B-cell lymphoma/leukemia 11B (BCL11B) gene in the human genome. Long range DNA sequencing confirmed the virus and flanking BCL11B DNA structures including both insertion junctions. Global transcriptomic analysis detected multiple, alternatively spliced, HPV70-BCL11B, fusion transcripts with fused open reading frames. The insertion and fusion transcripts were present in an intraepithelial precursor phase of tumorigenesis. These results suggest oncogenicity of HPV70, identify novel BCL11B variants with potential oncogenic implications, and underscore the advantages of thorough genomic analyses to elucidate insights into HPV-associated tumorigenesis.


Asunto(s)
Papillomaviridae/genética , Infecciones por Papillomavirus/diagnóstico , Neoplasias del Cuello Uterino/genética , Secuencia de Bases , Carcinogénesis/genética , Carcinogénesis/metabolismo , ADN Viral/análisis , Femenino , Genómica , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Humanos , Persona de Mediana Edad , Papillomaviridae/patogenicidad , Infecciones por Papillomavirus/genética , Reacción en Cadena de la Polimerasa , ARN Mensajero/genética , Proteínas Represoras/genética , Proteínas Supresoras de Tumor/genética , Neoplasias del Cuello Uterino/metabolismo
14.
Talanta ; 206: 120201, 2020 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-31514868

RESUMEN

Human immunodeficiency virus (HIV) is a lentivirus that leads to acquired immunodeficiency syndrome (AIDS). With increasing awareness of AIDS emerging as a global public health threat, different HIV testing kits have been developed to detect antibodies (Ab) directed toward different parts of HIV. A great limitation of these tests is that they can not detect HIV antibodies during early virus infection. Therefore, to overcome this challenge, a wide range of biosensors have been developed for early diagnosis of HIV infection. A significant amount of these studies have been focused on the application of nanomaterials for improving the sensitivity and accuracy of the sensing methods. Following an introduction into this field, a first section of this review covers the synthesis and applicability of such nanomaterials as metal nanoparticles (NPs), quantum dots (QDs), carbon-based nanomaterials and metal nanoclusters (NCs). A second larger section covers the latest developments concerning nanomaterial-based biosensors for HIV diagnosis, with paying a special attention to the determination of CD4+ cells as a hall mark of HIV infection, HIV gene, HIV p24 core protein, HIV p17 peptide, HIV-1 virus-like particles (VLPs) and HIV related enzymes, particularly those that are passed on from the virus to the CD4+ T lymphocytes and are necessary for viral reproduction within the host cell. These studies are described in detail along with their diverse principles/mechanisms (e.g. electrochemistry, fluorescence, electromagnetic-piezoelectric, surface plasmon resonance (SPR), surface enhanced Raman spectroscopy (SERS) and colorimetry). Despite the significant progress in HIV biosensing in the last years, there is a great need for the development of point-of-care (POC) technologies which are affordable, robust, easy to use, portable, and possessing sufficient quantitative accuracy to enable clinical decision making. In the final section, the focus is on the portable sensing devices as a new standard of POC and personalized diagnostics.


Asunto(s)
Técnicas Biosensibles/métodos , Infecciones por VIH/diagnóstico , VIH , Nanoestructuras/química , Anticuerpos Antivirales/análisis , Anticuerpos Antivirales/inmunología , Biomarcadores/análisis , ADN Viral/análisis , Diagnóstico Precoz , VIH/química , VIH/genética , VIH/inmunología , Humanos , Pruebas en el Punto de Atención , ARN Viral/análisis , Proteínas Virales/análisis
15.
Talanta ; 206: 120205, 2020 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-31514877

RESUMEN

We report an attomolar sensitive electrochemical genosensor for the detection of cauliflower mosaic virus 35S (CaMV35S) gene. The sandwich-type genosensor uses gold-silver core-shell (Au@Ag)-loaded iron oxide (Fe3O4) nanocomposite (Fe3O4-Au@Ag) as label of signal DNA probe (sDNA). Electrochemical sensing is accomplished at interface of electrodeposited AuNPs and carboxylated multiwalled carbon nanotubes-modified glassy carbon electrode through the specific interaction between the capture probe and target CaMV35S (tDNA), and tDNA and the labeled sDNA. The detection sensitivity was improved by the amplified reduction signal of hydrogen peroxide (H2O2), which takes advantage of the enhanced electrocatalytic activity of Fe3O4-Au@Ag. Under the optimal experimental conditions, an ultralow limit of detection was calculated to be 1.26 × 10-17 M (S/N = 3), and the blank value subtracted reduction signal of H2O2 of the sensor increased linearly with the logarithm of CaMV35S concentration over a wide range (1 × 10-16 M to 1 × 10-10 M). This genosensor displayed excellent stability, selectivity and reproducibility, and was successful in detecting the target CaMV35S in genetically modified tomato samples.


Asunto(s)
Técnicas Biosensibles/métodos , Caulimovirus/genética , ADN Viral/análisis , Nanopartículas de Magnetita/química , Nanocompuestos/química , Plantas Modificadas Genéticamente/genética , Sondas de ADN/química , Sondas de ADN/genética , ADN Viral/genética , Técnicas Electroquímicas/instrumentación , Técnicas Electroquímicas/métodos , Electrodos , Oro/química , Peróxido de Hidrógeno/química , Lycopersicon esculentum/genética , Nanotubos de Carbono/química , Hibridación de Ácido Nucleico , Oxidación-Reducción , Plata/química
16.
Talanta ; 207: 120308, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31594570

RESUMEN

Given the threat that ostreid herpesvirus 1 (OsHV-1) poses to shellfish aquaculture, the need for rapid, user-friendly and cost-effective methods to detect this marine pathogen and minimise its impact is evident. In this work, an electrochemical biosensor for the detection of OsHV-1 based on isothermal recombinase polymerase amplification (RPA) was developed. The system was first tested and optimised on maleimide microtitre plates as a proof-of-concept, before being implemented on miniaturised gold electrodes. Amperometric detection of the isothermally amplified product was achieved through a sandwich hybridisation assay with an immobilised thiolated capture probe and a horseradish peroxidase (HRP)-labelled reporter probe. Calibration curves were constructed using PCR-amplified OsHV-1 DNA, achieving a limit of detection of 207 OsHV-1 target copies. The biosensor was applied to the analysis of 16 oyster samples from an infectivity experiment, and results were compared with those obtained by qPCR analysis, showing a strong degree of correlation (r = 0.988). The simplicity, rapidity, cost-effectiveness and potential for in-situ testing with the developed biosensor provide a valuable tool for the detection of OsHV-1 in aquaculture facilities, improving their management.


Asunto(s)
Técnicas Biosensibles/métodos , Crassostrea/virología , Virus ADN/genética , ADN Viral/análisis , ADN Viral/genética , Miniaturización , Temperatura , Animales , Técnicas Biosensibles/economía , Calibración , Calorimetría , Análisis Costo-Beneficio , Electroquímica , Electrodos , Límite de Detección , Técnicas de Amplificación de Ácido Nucleico , Factores de Tiempo
17.
Talanta ; 207: 120290, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31594607

RESUMEN

Human T-lymphotropic virus type II (HTLV-II) is a crucial retrovirus that is closely associated with a variety of human diseases. Herein, an ultrasensitive fluorescent HTLV-II DNA detection strategy was developed for the first time based on magnetic nanoparticles (MNPs) and atom transfer radical polymerization (ATRP) amplification. In this approach, hairpin DNA probes (pDNA) labelled with 5' thiol and 3' azide group terminally were immobilized on amino group modified MNPs surface through sulfo-N-succinimidyl-4-maleimidobutyrate sodium salt (sulfo-GMBS) cross-linkers. In the presence of target DNAs (tDNA), pDNA hybridized with tDNA to form double-stranded DNA, and therefore its azide group was away from the MNPs surface. Subsequently, to initiate ATRP reaction, initiators were introduced into the pDNA by a Cu (I)-catalyzed alkyne-azide cycloaddition (CuAAC). Then, large numbers of 9-anthracenylmethyl methacrylate polymer (pAMMA) were successfully labelled on the MNPs surface, resulting in significant amplification of the fluorescence signal. Under optimized conditions, the fluorescence signal was proportional to the logarithm of the concentration of tDNA over the range from 1 fM to 1 nM, with a detection limit of 0.22 fM. Moreover, this strategy was capable of discriminating mismatched bases and detecting HTLV-II DNA in human serum samples. By virtue of the high sensitivity, selectivity, simplicity and economy, this ultrasensitive biosensor demonstrates great potential for biomedical research and early clinical diagnosis.


Asunto(s)
Técnicas Biosensibles/métodos , ADN Viral/análisis , Virus Linfotrópico T Tipo 2 Humano/genética , Límite de Detección , Imanes/química , Nanopartículas/química , Polimerizacion , ADN Viral/sangre , ADN Viral/química , Humanos , Hibridación de Ácido Nucleico , Espectrometría de Fluorescencia
18.
Medicine (Baltimore) ; 98(51): e17936, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31860948

RESUMEN

Clearance of the hepatitis B surface antigen (HBsAg) is the ultimate aim of treatment for patients with chronic hepatitis B (CHB) infection. Genetic, factor age, and gender were reported to be involved in the clearance of HBsAg. However, the rate of HBsAg seroclearance in CHB patients is still low globally and few of the single-nucleotide polymorphism (SNP) had been identified to associated with HBsAg seroclearance in CHB patients.Recently, 3 associated SNPs (rs7944135, rs171941, and rs6462008) were reported in the clearance of HBsAg in the Korean population. However, these SNPs have not been investigated in the CHB Taiwanese population. In present study, these 3 SNPs were genotyped in 2565 Taiwanese CHB patients including 493 CHB patients with HBsAg seroclearance and 2072 without HBsAg seroclearance.We observed that SNP rs7944135 was solely associated with HBsAg seroclearance. Subjects with the AA genotype at rs7944135 of macrophage-expressed gene 1 had a higher susceptibility to HBsAg clearance, compared to those with the AG or GG genotype under the genotypic model (odds ratio [OR] = 1.76. 95% confidence interval [CI] = 1.14-2.72, P = .045). Furthermore, we found a 1.74-fold increased risk of acquiring HBsAg seroclearance associated with the AA genotype compared to AG + GG of rs7944135 under the recessive model (OR = 1.74. 95% CI = 1.13-2.66, P = .014). According to the cumulative fraction curve with the log-rank test revealed that patients with the AA genotype of rs7944135 showed higher susceptibility to occur HBsAg seroclearance (P = .039) and HBV DNA undetectable (P = .0074) compared to those with the AG or GG genotype.This study examined the associations of 3 SNPs (rs7944135, rs171941, and rs6462008) with HBsAg seroclearance, and we identified that rs7944135 is solely associated with HBsAg seroclearance in Taiwanese CHB patients.


Asunto(s)
Regulación de la Expresión Génica , Antígenos de Superficie de la Hepatitis B/genética , Virus de la Hepatitis B/genética , Hepatitis B Crónica/genética , Polimorfismo de Nucleótido Simple/genética , Adulto , Estudios de Cohortes , Intervalos de Confianza , ADN Viral/análisis , ADN Viral/genética , Femenino , Genotipo , Antígenos de Superficie de la Hepatitis B/inmunología , Hepatitis B Crónica/inmunología , Hepatitis B Crónica/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Polimorfismo de Nucleótido Simple/inmunología , Estudios Retrospectivos , Pruebas Serológicas/métodos , Taiwán
19.
Rev Chilena Infectol ; 36(4): 421-427, 2019 Aug.
Artículo en Español | MEDLINE | ID: mdl-31859764

RESUMEN

BACKGROUND: The early onset of sexual activity can promote the development of cervical alterations and sexually transmitted infections, especially the human papillomavirus (HPV) very common in adolescents and young people. AIM: The condition of the cervix, HPV and sexual behavior in young women under 25 years of age were analyzed. METHODS: 182 university students, healthy, sexually active, 18-24 years old, without vaccine for HPV participated. Papanicolaou (Pap) test and classification of high and low risk HPV (HR and LR) were performed by real time polymerase chain reaction. The sexual behaviors were consulted in private. RESULTS: The 46.9% of Pap presented cytological alterations, non-specific inflammation/hemorrhagic (29.4%) and atypical smear (10.2%) being de most frequent. The overall frequency of HPV-HR was 24.3%, of these 67.4% presented an altered Pap. There was an association between cytological alterations and HPV (p < 0.0001) and years of sexual activity and atypical smear or cervical intraepithelial neoplasia grade I (CIN I) (p = 0.009). 11.9% of young women (21/177) presented atypical smear or CIN I, with 66.7% of cases HPV-HR. CONCLUSIONS: These findings alert the vulnerability of these young women who would have a potential risk of viral persistence, CIN and eventually cancer. It is important to emphasize counseling and prevention prior to the regular age of admission to the screening program for cervical cancer. This study was financed by the Universidad de La Frontera through Projects DI15-0047 and DI17-0123.


Asunto(s)
ADN Viral/análisis , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/diagnóstico , Conducta Sexual/estadística & datos numéricos , Estudiantes/estadística & datos numéricos , Adolescente , Chile/epidemiología , Femenino , Humanos , Tamizaje Masivo , Prueba de Papanicolaou , Papillomaviridae/genética , Infecciones por Papillomavirus/epidemiología , Reacción en Cadena de la Polimerasa , Factores de Riesgo , Universidades , Adulto Joven
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