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1.
Zhongguo Zhong Yao Za Zhi ; 46(5): 1026-1033, 2021 Mar.
Artículo en Chino | MEDLINE | ID: mdl-33787093

RESUMEN

Due to worldwide abuse of chemical antibiotics and continuous emergence of "superbugs", the harm of bacterial drug resistance to human beings has become more and more serious. Therefore, it is of great significance to look for green antibiotics with a wide range of sources, broad antibacterial spectrum, non-toxicity or low toxicity, environmentally friendliness, diverse active components and low drug resistance. The volatile oil of traditional Chinese medicine is a kind of volatile oily liquid that exists in plants and can be distilled with steam and immiscible with water. Because of its good potential to resist drug-resistant pathogens, it is widely used in food, medicine and other fields. This paper summarized the antibacterial advantages and characteristics of volatile oil of traditional Chinese medicine, and the antibacterial effect and antibacterial mechanism of combined application of volatile oil of traditional Chinese medicine, in order to provide some theoretical basis and study ideas for solving the problem of bacterial drug resistance and developing natural and green antibiotics.


Asunto(s)
Antiinfecciosos , Medicamentos Herbarios Chinos , Aceites Volátiles , Antibacterianos/farmacología , Medicamentos Herbarios Chinos/farmacología , Humanos , Medicina China Tradicional , Aceites Volátiles/farmacología
2.
Zhongguo Zhong Yao Za Zhi ; 46(5): 1141-1147, 2021 Mar.
Artículo en Chino | MEDLINE | ID: mdl-33787108

RESUMEN

The volatile oil from Mastiche and Olibanum medicinal materials was extracted by steam distillation, and the chemical components of the volatile oil were analyzed by GC-MS technology. The differences of the volatile oil components were compared and study on the Helicobacter pylori in vitro antimicrobial activitiy was conducted. The results showed that the yields of the volatile oil from Mastiche and Olibanum were 11.93% and 2.40%, respectively. A total of 46 compounds(91.31%) were identified from the volatile oil from Mastiche annd 35 compounds(92.49%) from Olibanum. The classification and comparison study of the components showed that the content of monoterpenes in the volatile oil from Mastiche was the highest(40.69%), followed by alcohols(28.48%); while the content of alcohols in the volatile oil from Olibanum was the highest(35.81%), followed by esters(24.92%). There were significant differences in the components of volatile oil from Mastiche and Olibanum, which might be one of the reasons for the difference in efficacy and application. In vitro bacteriostatic experiments showed that the minimum inhibitory concentration(MIC) of the volatile oil from Mastiche against H. pylori was 1 mg·mL~(-1), and the MIC of the volatile oil from Olibanum against H. pylori was more than 1 mg·mL~(-1). In combination with the results of the oil yield experiment, Mastiche had the advantage of inhibiting H. pylori activity. The research results provide scientific basis for the rational application of Mastiche and Olibanum.


Asunto(s)
Olíbano , Helicobacter pylori , Aceites Volátiles , Antibacterianos/farmacología , Cromatografía de Gases y Espectrometría de Masas , Monoterpenos/análisis , Aceites Volátiles/farmacología
3.
Int J Food Microbiol ; 344: 109104, 2021 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-33676333

RESUMEN

The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO). Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C). Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study. The bacterial count was performed on days 0, 1, 2, 4, 8, and 12. Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05). Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage. According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.


Asunto(s)
Antibacterianos/farmacología , Conservación de Alimentos/métodos , Listeria monocytogenes/crecimiento & desarrollo , Aceites Volátiles/farmacología , Aceites Vegetales/farmacología , Pavos/microbiología , Alginatos/farmacología , Animales , Apiaceae/química , Recuento de Colonia Microbiana , Películas Comestibles , Emulsiones/química , Contaminación de Alimentos/prevención & control , Humanos , Listeria monocytogenes/efectos de los fármacos , Carne/microbiología
4.
An Acad Bras Cienc ; 93(1): e20201507, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33656062

RESUMEN

Phytomedicines are becoming more popular in treatment of infectious diseases worldwide. Cinnamomum verum essential oil (EO) has been used as a therapeutic alternative for various diseases. This study aimed to evaluate the antibacterial and antibiofilm activity of the C. verum leaf EO against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae. Effect of EO vapor on planktonic cells was determined using microatmosphere technique. CLSI M7-A10 method was employed in Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) experiments. Effect of EO on established biofilms was quantified and visualized using XTT and Scanning Electron Microscope (SEM). In-vitro toxicity was evaluated using Human Keratinocytes (HaCaT). Chemical analysis of EO was done using Gas Chromatography- Mass Spectrometry (GC-MS). All tested strains were sensitive to cinnamon oil vapor. EO exhibited 0.5 and 1.0 mg/mL MIC and MBC against all test strains. Minimum Biofilm Inhibitory and Biofilm Eradication Concentrations (MBIC50 and MBEC) were 1.0 and 4.0 mg/mL. SEM indicated cellular shrinkages, cell wall damages, and decreased biofilm densities. Cinnamon oil didn't show any toxicity on HaCaT cell at any concentration tested. Eugenol was the most abundant compound in C. verum oil. C. verum EO shows an antibacterial and antibiofilm activity with minimal toxicity on host.


Asunto(s)
Aceites Volátiles , Staphylococcus aureus , Antibacterianos/farmacología , Biopelículas , Cinnamomum zeylanicum , Cromatografía de Gases y Espectrometría de Masas , Humanos , Klebsiella pneumoniae , Pruebas de Sensibilidad Microbiana , Aceites Volátiles/farmacología , Hojas de la Planta , Pseudomonas aeruginosa
5.
Molecules ; 26(5)2021 Feb 26.
Artículo en Inglés | MEDLINE | ID: mdl-33652584

RESUMEN

The purpose of the research was to examine the protective effect of essential oil from Thymus serrulatus Hochst. ex Benth. (TSA oil) against cadmium (Cd)-induced renal toxicity. The experimental protocol was designed using 30 healthy adult Wistar albino rats allocated into five groups containing six animals in each group. Group 1 was treated as normal control and groups 2, 3, 4, and 5 were treated with cadmium chloride (CdCl2, 3 mg/kg, IP) for 7 days. Group 3 was also treated with silymarin (100 mg/kg, PO) as a standard group, while groups 4 and 5 were administered with TSA oil at doses of 100 and 200 mg/kg PO, respectively. The nephrotoxicity was measured with various parameters such as kidney function markers, oxidative stress markers (glutathione (GSH) and malondialdehyde (MDA)), and messenger ribonucleic acid (mRNA) expression levels of inflammatory factors. The histological studies were also evaluated in the experimental protocol. The CdCl2-treated groups showed a significant increase in the levels of serum kidney function markers along with MDA levels in kidney homogenate. However, renal GSH level was found to be reduced significantly. It was found that CdCl2 significantly upregulated the nuclear factor levels of kappaB (NF-κB p65), inducible nitric oxide synthase (iNOS), and small mothers against decapentaplegic (Smad2) as compared to the normal control group. On the other hand, TSA oil significantly improved the increased levels of serum kidney function markers, non-enzymatic antioxidants, and lipid peroxidation. In addition, TSA oil significantly downregulated the increased expression of NF-κB p65, iNOS, and Smad2 in Cd-intoxicated rats. Moreover, the histological changes in the tissue samples of the kidney of Cd-treated groups were significantly ameliorated in the silymarin- and TSA-oil-treated groups. The present study reveals that TSA oil ameliorates Cd-induced renal injury, and it is also proposed that the observed nephroprotective effect could be due to the antioxidant potential of TSA oil and healing due to its anti-inflammatory action.


Asunto(s)
Enfermedades Renales/tratamiento farmacológico , Aceites Volátiles/química , Estrés Oxidativo/efectos de los fármacos , Thymus (Planta)/química , Animales , Antioxidantes/química , Antioxidantes/farmacología , Cadmio/toxicidad , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Riñón/efectos de los fármacos , Riñón/patología , Enfermedades Renales/inducido químicamente , FN-kappa B/genética , Óxido Nítrico Sintasa de Tipo II/genética , Aceites Volátiles/farmacología , Ratas , Proteína Smad2/genética
6.
Molecules ; 26(4)2021 Feb 13.
Artículo en Inglés | MEDLINE | ID: mdl-33668450

RESUMEN

Greek oregano and common oregano were compared in respect of the antioxidant and antibacterial activity of the corresponding essential oils and hydroethanolic extracts in relation with their chemical profile. The chemical composition of essential oils was determined by GC-MS and GC-FID, while extracts (phenolic acids and flavonoids fractions) were analyzed by HPLC-DAD. Based on given volatiles, the investigated subspecies represented two chemotypes: a carvacrol/γ-terpinene/p-cymene type in the case of Greek oregano and a sabinyl/cymyl type rich in terpinen-4-ol in common oregano. Within non-volatile phenolic compounds, rosmarinic acid appeared to dominate in both subspecies. Lithospermic acid B, chlorogenic acid and isovitexin were present only in Greek oregano extracts. However, the total content of flavonoids was higher in common oregano extracts. The essential oil and extract of Greek oregano revealed visibly stronger antibacterial activity (expressed as MIC and MBC) than common oregano, whereas the antioxidant potential (determined by DPPH, ABTS and FRAP) of these extracts was almost equal for both subspecies. In the case of Origanum plants, the potential application of essential oils and extracts as antiseptic and antioxidant agents in the food industry should be preceded by subspecies identification followed by recognition of their chemotype concerning both terpene and phenolics composition.


Asunto(s)
Antibacterianos/farmacología , Antioxidantes/farmacología , Etanol/química , Aceites Volátiles/farmacología , Origanum/química , Extractos Vegetales/farmacología , Agua/química , Benzotiazoles/química , Compuestos de Bifenilo/química , Cromatografía Líquida de Alta Presión , Flavonoides/análisis , Depuradores de Radicales Libres/farmacología , Pruebas de Sensibilidad Microbiana , Fenoles/análisis , Picratos/química , Reproducibilidad de los Resultados , Ácidos Sulfónicos/química
7.
Molecules ; 26(4)2021 Feb 19.
Artículo en Inglés | MEDLINE | ID: mdl-33669825

RESUMEN

The aim of the present research was to determine the chemical composition and the cytotoxic effects of Tetraclinis articulata trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the identification of 54 known compounds. Relatively high amounts of oxygenated sesquiterpenes (44.4-70.2%) were detected, mainly consisting of caryophyllene oxide (13.1-26.6%), carotol (9.2-21.2%),14-hydroxy-9-epi-(E)-caryophyllene (3.2-15.5%) and humulene epoxide II (2.6-7.2%). The cytotoxic activity against human mammary carcinoma cell lines (MDA-MB-231) and colorectal carcinoma cell lines (SW620) of the essential oil and its fractions were assessed. All the samples displayed moderate to weak activity compared to 5-fluorouracil. The colorectal carcinoma cell line was relatively more sensitive to the essential oil and its fractions compared to the breast cancer cell line, showing IC50 values from 25.7 to 96.5 µg/mL. In addition, the essential oil and its fraction E.2 revealed a cytotoxic activity against colorectal carcinoma cell line, with IC50 values lower than 30 µg/mL. This is the first report on the chemical composition and cytotoxic activity of the trunk bark essential oil of T. articulata.


Asunto(s)
Cupressaceae/química , Cupressaceae/crecimiento & desarrollo , Aceites Volátiles/química , Aceites Volátiles/farmacología , Corteza de la Planta/química , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Fraccionamiento Químico , Humanos , Concentración 50 Inhibidora , Túnez
8.
Molecules ; 26(4)2021 Feb 11.
Artículo en Inglés | MEDLINE | ID: mdl-33670315

RESUMEN

Arabian flora is a rich source of bioactive compounds. In this study, we investigated three aromatic plant species with the aim of finding valuable sources of antimicrobial agents against common pathogenic microorganisms. We focused especially on microorganisms, which cause outbreaks of infectious disease during mass gatherings and pilgrimages season in Saudi Arabia. The essential oils of three aromatic plant species were hydrodistilled from flowering aerial parts of Lavandula pubescens Decne. and Pulicaria incisa subsp. candolleana E.Gamal-Eldin, and from leaves, stems, ripe and unripe fruits of Juniperus procera Hochst. Ex Endl. They were subsequently analyzed by gas chromatography-mass spectrometry (GC-MS). The main constituents of L. pubescens were found to be carvacrol (55.7%), methyl carvacrol (13.4%), and ß-bisabolene (9.1%). P. incisa subsp. Candolleana essential oil was rich in linalool (33.0%), chrysanthenone (10.3%), eugenol (8.9%), and cis-chrysanthenol (8.0%); the major components of J. procera essential oil were α-pinene (31.3-62.5%) and δ-3-carene (7.3-30.3%). These essential oils were tested against thirteen American Type Culture Collection (ATCC) strains of Gram-positive and Gram-negative bacteria using the agar diffusion assay. The only effective essential oil was that of L. pubescens and the most sensitive strains were Acinetobacter baumannii, Salmonella typhimurium, Shigella sonnei, Enterococcus faecalis and Staphylococcus epidermidis. Carvacrol, the major constituent of L. pubescens, was tested on these strains and was compared with vancomycin, amikacin, and ciprofloxacin. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays of L. pubescens essential oil and carvacrol revealed that Gram-negative strains were more susceptible than the Gram-positive ones.


Asunto(s)
Juniperus/química , Lavandula/química , Aceites Volátiles/química , Aceites Vegetales/química , Pulicaria/química , Antibacterianos/química , Antibacterianos/farmacología , Cimenos/química , Cimenos/farmacología , Enterococcus faecalis/efectos de los fármacos , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , Aceites Volátiles/farmacología , Componentes Aéreos de las Plantas/química , Hojas de la Planta/química , Aceites Vegetales/farmacología , Arabia Saudita , Staphylococcus aureus/efectos de los fármacos
9.
Food Chem ; 352: 129319, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33691215

RESUMEN

Emulsions based on licorice essential oil (LEO) were prepared under different homogenization conditions (ultra-homogenization (U1) or U1 together with sonication (U2)). The obtained emulsions were incorporated into the carboxymethyl cellulose (CMC) film. The results showed that U2 caused significant changes in the size and the surface charge of the emulsions. Remarkable differences in the microstructure were observed between the U1 and the U2 emulsion-based films as revealed by SEM and AFM. Both emulsions reduced the rigidity and increased the flexibility of the films. The film made from the CMC alone had a water vapor permeability (WVP) of 2.66 × 10-9 g m-1 s-1 Pa-1, while the CMC film made from U2 emulsion had a WVP of 1.87 × 10-9 g m-1 s-1 Pa-1. Also, the film containing 0.0125% U1-LEO exhibited antibacterial activity on gram-positive bacteria only while the film containing 0.0125% U2-LEO demonstrated antibacterial activity on both gram-positive and gram-negative bacteria.


Asunto(s)
Antibacterianos/química , Antibacterianos/farmacología , Glycyrrhiza/química , Aceites Volátiles/química , Aceites Volátiles/farmacología , Sonicación , Carboximetilcelulosa de Sodio/química , Emulsiones , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Permeabilidad , Vapor
10.
Molecules ; 26(4)2021 Feb 20.
Artículo en Inglés | MEDLINE | ID: mdl-33672486

RESUMEN

Medicinal plants and their derived compounds have drawn the attention of researchers due to their considerable impact on human health. Among medicinal plants, mint (Mentha species) exhibits multiple health beneficial properties, such as prevention from cancer development and anti-obesity, antimicrobial, anti-inflammatory, anti-diabetic, and cardioprotective effects, as a result of its antioxidant potential, combined with low toxicity and high efficacy. Mentha species are widely used in savory dishes, food, beverages, and confectionary products. Phytochemicals derived from mint also showed anticancer activity against different types of human cancers such as cervix, lung, breast and many others. Mint essential oils show a great cytotoxicity potential, by modulating MAPK and PI3k/Akt pathways; they also induce apoptosis, suppress invasion and migration potential of cancer cells lines along with cell cycle arrest, upregulation of Bax and p53 genes, modulation of TNF, IL-6, IFN-γ, IL-8, and induction of senescence phenotype. Essential oils from mint have also been found to exert antibacterial activities against Bacillus subtilis, Streptococcus aureus, Pseudomonas aeruginosa, and many others. The current review highlights the antimicrobial role of mint-derived compounds and essential oils with a special emphasis on anticancer activities, clinical data and adverse effects displayed by such versatile plants.


Asunto(s)
Antibacterianos/farmacología , Antineoplásicos Fitogénicos/farmacología , Antioxidantes/farmacología , Mentha/química , Aceites Volátiles/farmacología , Fitoquímicos/farmacología , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Bacterias/efectos de los fármacos , Compuestos de Bifenilo/antagonistas & inhibidores , Proliferación Celular/efectos de los fármacos , Humanos , Aceites Volátiles/química , Aceites Volátiles/aislamiento & purificación , Fitoquímicos/química , Fitoquímicos/aislamiento & purificación , Picratos/antagonistas & inhibidores
11.
Animal ; 15(1): 100022, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33573947

RESUMEN

The addition of essential oil (EO) as chitosan encapsulated can increase the efficiency of these oils in broiler feeding. Therefore, the objective of the current research was to explore the antibacterial and antioxidant potential of garlic essential oil (GEO) (free vs. nanoencapsulated) and their effects on performance, gene expression of mucin2, microbial, and morphology of intestine in broilers. A total of 900 1-day-old male broilers (Ross 308) were assigned to six dietary treatments (0, 100, and 200 mg/kg free GEO and 0 (contain of chitosan), 100 and 200 mg/kg nanoencapsulated GEO) with a 2 × 3 factorial arrangement based on completely randomized design. Garlic essential oil encapsulation with chitosan significantly enhanced antibacterial and antioxidant parameters. At 100 mg/kg nanoencapsulated GEO had significant (P < 0.01) advantages in improving BW gain (BWG) (22-42 and 0-42) and feed conversion ratio (FCR) (0-42). Maximum feed intake (FI) was also associated with the control group (P < 0.05). Broilers fed on 100 mg/kg of nanoencapsulated GEO showed higher villi length and width relative to other treatments and villi length to crypt depth ratio as well (P < 0.01). The nanoencapsulation process of GEO (P < 0.01) affected the Lactobacilli population in the digesta of ileo-caecum and mucin2 gene expression. In broiler chickens, the tested EO, especially nanoencapsulated type, enhanced more evaluated parameters. Because of its ideal properties, nanoencasulating with chitosan may also be an effective and inexpensive way to protect bioactive compounds and improve GEO effects in broiler chickens.


Asunto(s)
Ajo , Aceites Volátiles , Alimentación Animal/análisis , Animales , Antibacterianos/farmacología , Antioxidantes , Pollos , Dieta/veterinaria , Suplementos Dietéticos/análisis , Masculino , Aceites Volátiles/farmacología , Tecnología
12.
J Anim Sci ; 99(2)2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-33544855

RESUMEN

Essential oils are plant-derived aromatic volatile oils, and they contain bioactive compounds that have been shown to improve poultry nutrition. In this study, we investigated the effects of oregano essential oil (OEO) on intestinal antioxidative capacity, immunity, and gut microbiota of young yellow-feathered chickens. A total of nine hundred and sixty 1-d-old female Qingyuan partridge chickens were randomly allocated to four treatment groups with six replicates of 40 birds each, and the feeding trial was lasted for 30 d. The controls were fed on a basal diet without in-feed antibiotics; the birds in the antibiotic group were fed the basal diet supplemented with 20 mg/kg virginiamycin; the remaining birds were fed the basal diet containing 150 or 300 mg/kg OEO, respectively. Dietary supplementation with 150 or 300 mg/kg OEO increased average daily feed intake (P = 0.057) and average daily gain (P < 0.05). The activities of glutathione peroxidase and total antioxidative capacity in plasma, jejuna, and ileal mucosa were increased by OEO supplementation (P < 0.05), with a trend of lower jejunal content of malonaldehyde (P = 0.062). Moreover, dietary OEO increased the content of secretory immunoglobulin A (P = 0.078) and the relative expression of Claudin 1, Mucin 2, and Avain beta-defensin 1 in ileum (P < 0.05). Sequencing data of 16S rRNA indicated that dietary OEO increased the relative abundance of Firmicutes phylum, and Clostridium and Lactobacillus genera, and decreasing that of Romboutsia. Functional analyses indicated that microbial amino sugar and nucleotide sugar metabolism, replication, and repair systems were higher in OEO groups than those of controls and antibiotic treatment. In conclusion, dietary supplementation with OEO enhanced growth performance, alleviated local oxidative stress in intestine, improved production of natural antibodies, and favorably modulated intestinal microbiota composition.


Asunto(s)
Microbioma Gastrointestinal , Aceites Volátiles , Origanum , Alimentación Animal/análisis , Animales , Antioxidantes , Pollos , Dieta/veterinaria , Suplementos Dietéticos , Femenino , Intestinos , Aceites Volátiles/farmacología , ARN Ribosómico 16S
13.
Pestic Biochem Physiol ; 172: 104755, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33518049

RESUMEN

The present study investigated the novel antifungal, and anti-aflatoxin B1 mechanism of Eugenia caryophyllata L. essential oil (ECEO) loaded chitosan nanomatrix against the toxigenic strain of A. flavus (AFLV-DK-02). Phytochemical profiling of ECEO was done by GC-MS which revealed eugenol (73.6%) as the primary bioactive compound. ECEO was encapsulated inside the chitosan nanomatrix (ECEO-Np) and characterized using SEM, AFM, FTIR and XRD analysis. The ECEO-Np exhibited enhance antifungal (0.25 µL/mL) and anti-aflatoxin B1 inhibitory activity (0.15 µL/mL) than ECEO. Antifungal and antiaflatoxin B1 inhibitory activity was found to be related with impairment in the biological functioning of the plasma membrane (ergosterol synthesis, leakage of membrane ions, UV light (260, 280 nm) absorbing material, dead cell by propidium iodide assay, mitochondrial membrane potential (MMP), methylglyoxal and inhibition in essential carbon substrate utilization). ECEO-Np exhibited remarkable free radical scavenging activity with IC50 value of 0.002 µL/mL. ECEO-Np effectively preserves the sensory characteristics of exposed maize crop seed up to six months of storage and shows considerable safety profile (non-toxic, non-mutagenic, non-hepatotoxic, non-carcinogenic, non-tumorigenic and biodegradable) using computational ADMET (absorption, distribution, metabolism, excretion, and toxicity) analysis.


Asunto(s)
Quitosano , Aceites Volátiles , Syzygium , Aflatoxina B1 , Antifúngicos/farmacología , Aspergillus flavus , Aceites Volátiles/farmacología
14.
Int J Food Microbiol ; 342: 109071, 2021 Mar 16.
Artículo en Inglés | MEDLINE | ID: mdl-33578302

RESUMEN

Biodegradable films reinforced with bio-nanomaterials are a solution for developing active packaging systems, shelf-life extension and protection of environment against conventional packaging. This study aimed to characterize the biocompatible chitosan (CS) films formulated with nano-liposomal garlic essential oil (NLGEO) and assess the physicho-mechanical, morphology properties and also microbial and chemical changes in chicken fillets during storage time at 4 °C. NLGEO was obtained by thin-layer hydration-sonication method using glycerol and tween 80 as plasticizer and emulsifier, respectively. Different levels (0, 0.5, 1 and 2%) of NLGEO with average size of ~101 nm were added into the chitosan matrix and films fabricated by casting method. The average size, polydispersity index and zeta potential were ~101 nm, 0.127 and -7.23, respectively. Control samples showed higher values for pH, total volatile nitrogen (TVN), peroxide value (PV), thiobarbituric acid-reactive substances (TBARS), and microbial count including total viable count (TVC), coliforms, Staphylococcus aureus and psychrotroph bacteria than treated samples. The films with higher NLGEO content represented stronger inhibitory effects. The incorporation of NLGEO improved the mechanical properties and water resistance of active films. Microstructure analysis also showed a nearly smooth surface morphology and homogenous structure with a good dispersion for NLGEO films. Significant synergistic effects in chemical and bacterial preservation of chicken fillet samples were observed by NLGEO films. The optimal mechanical and barrier properties of chitosan-NLGEO films introduced it a potential active packaging to extend the shelf life of chicken fillet.


Asunto(s)
Quitosano/química , Embalaje de Alimentos/métodos , Nanocompuestos/química , Aceites Volátiles/química , Productos Avícolas/análisis , Productos Avícolas/microbiología , Compuestos Alílicos/química , Animales , Bacterias/clasificación , Bacterias/efectos de los fármacos , Bacterias/crecimiento & desarrollo , Pollos , Quitosano/farmacología , Conservación de Alimentos , Aceites Volátiles/farmacología , Sulfuros/química
15.
Environ Sci Pollut Res Int ; 28(15): 18918-18940, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33609244

RESUMEN

The control of storage insect pests is largely based on synthetic pesticides. However, due to fast growing resistance in the targeted insects, negative impact on humans and non-target organisms as well as the environment, there is an urgent need to search some safer alternatives of these xenobiotics. Many essential oils (EOs) and their bioactive compounds have received particular attention for application as botanical pesticides, since they exhibited high insecticidal efficacy, diverse mode of action, and favourable safety profiles on mammalian system as well as to the non-target organisms. Data collected from scientific articles show that these EOs and their bioactive compounds exhibited insecticidal activity via fumigant, contact, repellent, antifeedant, ovicidal, oviposition deterrent and larvicidal activity, and by inhibiting/altering important neurotransmitters such as acetylcholine esterase (AChE) and octopamine or neurotransmitter inhibitor γ-amino butyric acid (GABA), as well as by altering the enzymatic [superoxide dismutase (SOD), catalase (CAT), peroxidases (POx), glutathione-S-transferase (GST) and glutathione reductase (GR)] and non-enzymatic [glutathione (GSH)] antioxidant defence systems. However, in spite of promising pesticidal efficacy against storage pests, the practical application of EOs and their bioactive compounds in real food systems remain rather limited because of their high volatility, poor water solubility and susceptibility towards degradation. Nanoencapsulation/nanoemulsion of EOs is currently considered as a promising tool that improved water solubility, enhanced bio-efficacy, stability and controlled release, thereby expanding their applicability.


Asunto(s)
Repelentes de Insectos , Insecticidas , Aceites Volátiles , Plaguicidas , Animales , Insectos , Aceites Volátiles/farmacología
16.
Molecules ; 26(3)2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-33573272

RESUMEN

Pears (Pyrus communis L.) cv. Packham's Triumph are very traditional for human consumption, but pear is a highly perishable climacteric fruit with a short shelf-life affected by several diseases with a microbial origin. In this study, a protective effect on the quality properties of pears was evidenced after the surface application of chitosan-Ruta graveolens essential oil coatings (CS + RGEO) in four different concentrations (0, 0.5, 1.0 and 1.5 %, v/v) during 21 days of storage under 18 °C. After 21 days of treatment, a weight loss reduction of 10% (from 40.2 ± 5.3 to 20.3 ± 3.9) compared to the uncoated pears was evident with CS + RGEO 0.5%. All the fruits' physical-chemical properties evidenced a protective effect of the coatings. The maturity index increased for all the treatments. However, the pears with CS + RGEO 1.5% were lower (70.21) than the uncoated fruits (98.96). The loss of firmness for the uncoated samples was higher compared to the coated samples. The pears' most excellent mechanical resistance was obtained with CS + RGEO 0.5% after 21 days of storage, both for compression resistance (7.42 kPa) and force (22.7 N). Microbiological studies demonstrated the protective power of the coatings. Aerobic mesophilic bacteria and molds were significantly reduced (in 3 Log CFU/g compared to control) using 15 µL/mL of RGEO, without affecting consumer perception. The results presented in this study showed that CS + RGEO coatings are promising in the post-harvest treatment of pears.


Asunto(s)
Quitosano/química , Conservación de Alimentos/métodos , Aceites Volátiles/farmacología , Pyrus/química , Quitosano/farmacología , Respuesta al Choque por Frío/efectos de los fármacos , Frutas/química , Hongos/efectos de los fármacos , Humanos , Aceites Volátiles/química , Aceites Vegetales/química , Pyrus/efectos de los fármacos , Pyrus/microbiología , Ruta/química , Temperatura
17.
Int J Food Microbiol ; 341: 109048, 2021 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-33486390

RESUMEN

Yeasts are the leading cause of spoilage in yogurt. Considering the high demand from consumers to use natural products as an alternative to additives, essential oils (EOs) could be a promising solution to guarantee high microbiological standards. The present study highlighted the in vitro antifungal potential of cinnamon, ginger, lemongrass, mandarin, orange, lemon and lime EOs against spoilage yeasts isolated from yogurts prepared with pasteurized buffalo milk. A total of 74 isolates represented by 14 different species of Candida, Rhodotorula, Debaryomyces, Kluyveromyces and Yarrowia genera were subjected to a disc diffusion assay, showing lemongrass EO to have the highest antifungal activity (40.97 ± 9.86 mm), followed by cinnamon (38.46 ± 6.59 mm) and orange (12.00 ± 4.52 mm) EOs. Yarrowia lipolytica was less susceptible to lemongrass EO than Candida sake and Yarrowia deformans isolates. Ginger EO exhibited the lowest efficacy. A minimum inhibitory concentration (MIC) assay showed the ability of lemongrass and cinnamon EOs to inhibit the growth of all selected isolates at concentrations between ≤0.31 and 1.25 µL/mL. Therefore, for the first time, the two best-performing EOs (lemongrass and cinnamon) based on in vitro assays were assessed for their potential roles as preservatives in an in vivo yogurt model prepared at the laboratory scale. Since some limitations, such as the inhibition of lactic acid bacteria by cinnamon EO, consequently leading to fermentation failure as well as species-specific antifungal activity of lemongrass EO, were observed, further studies are needed to explore the possibility of using a slightly higher concentration of lemongrass EO and/or combinations of different EOs and/or their components. Finally, since yogurt spoilage could also be prevented by correct sanitation procedures of the production environment, the sanitizers commonly used in the food industry were tested against all isolates, showing the high efficiency of alcohol-based sanitizers and the ineffectiveness of chlorine-based sanitizers.


Asunto(s)
Antifúngicos/farmacología , Cinnamomum zeylanicum/química , Cymbopogon/química , Aceites Volátiles/farmacología , Levaduras/crecimiento & desarrollo , Yogur/microbiología , Candida/efectos de los fármacos , Candida/crecimiento & desarrollo , Candida/aislamiento & purificación , Citrus sinensis/química , Pruebas Antimicrobianas de Difusión por Disco , Contaminación de Alimentos/análisis , Saccharomycetales/efectos de los fármacos , Saccharomycetales/crecimiento & desarrollo , Saccharomycetales/aislamiento & purificación , Levaduras/efectos de los fármacos , Levaduras/metabolismo
18.
Int J Food Microbiol ; 341: 109074, 2021 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-33508583

RESUMEN

Campylobacter jejuni (C. jejuni) is one of the most common foodborne pathogens that cause human sickness mostly through the poultry food chain. Cinnamon essential oil (CEO) has excellent antibacterial ability against C. jejuni growth. This study investigated the antibacterial mechanism of CEO against C. jejuni primarily through metabolism, energy metabolism of essential enzymes (AKPase, ß-galactosidase, and ATPase), and respiration metabolism. Results showed that the hexose monophosphate pathway (HMP) was inhibited, and that the enzyme activity of G6DPH substantially decreased upon treatment with CEO. Analysis of the effect of CEO on the expression of toxic genes was performed by the real-time PCR (RT-PCR). The expression levels of the toxic genes cadF, ciaB, fliA, and racR under CEO treatment were determined. Casein/CEO nanospheres were further prepared for the effective inhibition of C. jejuni and characterized by particle-size distribution, zeta-potential distribution, fluorescence, TEM, and GC-MS methods. Finally, the efficiency of CEO and casein/CEO nanospheres in terms of antibacterial activity against C. jejuni was verified. The casein/CEO nanospheres displayed high antibacterial activity on duck samples. The population of the test group decreased from 4.30 logCFU/g to 0.86 logCFU/g and 4.30 logCFU/g to 2.46 logCFU/g at 4 °C and at 25 °C for C. jejuni, respectively. Sensory evaluation and texture analysis were also conducted on various duck samples.


Asunto(s)
Antibacterianos/farmacología , Infecciones por Campylobacter/veterinaria , Campylobacter jejuni/efectos de los fármacos , Caseínas/farmacología , Cinnamomum zeylanicum/química , Aceites Volátiles/farmacología , Animales , Infecciones por Campylobacter/tratamiento farmacológico , Pollos/microbiología , Preparaciones de Acción Retardada/farmacología , Patos/microbiología , Metabolismo Energético/efectos de los fármacos , Enfermedades Transmitidas por los Alimentos/microbiología , Enfermedades Transmitidas por los Alimentos/prevención & control , Expresión Génica/efectos de los fármacos , Glucosafosfato Deshidrogenasa/antagonistas & inhibidores , Humanos , Nanosferas , Vía de Pentosa Fosfato/efectos de los fármacos , Aves de Corral/microbiología
19.
Int J Food Microbiol ; 341: 109047, 2021 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-33515813

RESUMEN

Nowadays, adding biological compounds to food packaging is one of the types of active packaging. The aim of this study was to prepare a new degradable coating with free and nano-encapsulated Cumino cyminum L. essential oil (CCEO) with nanochitosan (Nch) base to evaluate the microbial, chemical and sensory properties of sardine fillet samples for 16 days at 4 °C. Nanoliposome using different soy lecithin ratios and cholesterol concentrations (60:0, 50:10, 40:20, and 30:30) and technique of thin-film hydration-sonication, were prepared with a range of 140-164 nm size. Encapsulation efficiency (EE) and distribution of nanoliposomes size were calculated 0.80-0.90 and 49.85-73.01% respectively. To coat sardine fillet samples, nanoliposomes with the lower size of droplet and higher EE percent were selected. The outcomes indicated that coating treatments can effectively inhibit microbial growth and chemical spoilage reflected at lower pH, peroxide value (PV) and thiobarbituric acid reactive substances (TBARs) (P < 0.05). In fact, the results of chemical and microbiological characteristics showed that the samples treated with nanocitosan/nano essential oil (NEO) showed the lowest value among other treatments during the experimental period, in the following, Nch-EO, Nch and control. pH, PV, TBARs, total viable counts (TVC), total pseudomonads count (TPC), and lactic acid bacteria (LAB) were 6.85, 0.03 (mg MDA/kg), 5.23 (mEq/kg), 3.67 (CFU/g), 3.47 (CFU/g), and 4.7 (CFU/g), respectively for Nch-NEO at the end of storage time. In addition, during the experimental period, the highest sensory properties were obtained for the Nch-NEO group. Encapsulation of CCEO reduces the rate of diffusion, thus increasing antimicrobial and antioxidant activity, as well as improving sensory properties. According to the results of this study, CCEO-encapsulated nanochitosan coatings can be used as a potent coating to increase sardine shelf life.


Asunto(s)
Antibacterianos/farmacología , Quitosano/farmacología , Cuminum/química , Embalaje de Alimentos/métodos , Aceites Volátiles/farmacología , Alimentos Marinos/microbiología , Animales , Antioxidantes/química , Antioxidantes/farmacología , Carga Bacteriana/efectos de los fármacos , Quitosano/química , Colesterol/farmacología , Peces/microbiología , Conservación de Alimentos/métodos , Almacenamiento de Alimentos/métodos , Lecitinas/farmacología
20.
Exp Appl Acarol ; 83(2): 301-312, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33389348

RESUMEN

Ticks are of great economic importance to humans and animals due to their role in disease transmission. The application of synthetic, chemical acaricides on the animal and/or the environment (the most used tick control method globally) has led to the selection of tick populations that are resistant. Their adverse effects on ecology and human and animal health cannot be overemphasised. As a result, the search for alternatives that are natural and can overcome these adverse effects are strongly indicated. Using gas chromatography-mass spectrometry (GC-MS) and adult immersion test (AIT), this study evaluated the chemical composition and acaricidal activity, respectively, of Eucalyptus globulus essential oil (EO) on Rhipicephalus (Boophilus) annulatus ticks. This is a major tick species implicated for the transmission of bovine piroplasmosis in Nigeria. The acaricidal activity was evaluated using different concentrations (0.625, 1.25, 2.5, 5 and 10%) of E. globulus EO. Amitraz (1 and 2%) and cypermethrin (2%) served as the positive control and 2% dimethylsulfoxide in distilled water was the negative control. Three replicates of 10 engorged female ticks each were immersed in the test samples for 2 min and the experiment was done twice. The GC-MS analysis identified the major constituents of E. globulus EO as eucalyptol (1,8-cineole) (78%), menthol (20%) and menthone (3%). Eucalyptus globulus EO caused 97% acaricidal mortality at 10% concentration. The lower concentrations reduced tick fecundity up to 90% in a dose-dependent manner. This study provides support for plant EOs as alternative tick control strategy for humans and animals.


Asunto(s)
Acaricidas , Babesiosis , Enfermedades de los Bovinos , Eucalyptus , Aceites Volátiles , Rhipicephalus , Animales , Bovinos , Aceite de Eucalipto , Femenino , Larva , Nigeria , Aceites Volátiles/farmacología
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