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1.
Sci Total Environ ; 803: 149744, 2022 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-34482147

RESUMEN

Pharmaceuticals (PhACs) are considered emerging contaminants with potential accumulation in aquatic organisms. Thus, seafood consumption may cause long-term effects and health risk for consumers. In the present study, the occurrence of PhACs in seafood from two Brazilian coastal areas, Sepetiba Bay (n = 43) and Parnaiba Delta River (n = 48), was determined for the first time, and their potential risk for human health was assessed. An eco-friendly multi-analytes method was used, after being validated for the different types of matrices (mussels, fatty and lean fish). All compounds under study were detected at least in four seafood species, including chloramphenicol, an antibiotic prohibited in animal foods. Most PhACs had mean concentrations below limit of quantification. Ibuprofen and other nonsteroidal anti-inflammatory drugs (NSAIDs), as well as simvastatin and carbamazepine were the main PhACs bioaccumulated in edible parts of seafood species from Brazil. The high trophic level carnivorous species, snook, was the most contaminated by NSAIDs, while bivalves were the seafood more contaminated by lipid regulators. The profile of contamination did not vary among different types of matrix, except in relation to carbamazepine and ketoprofen. These PhACs were more abundant in species from Sepetiba Bay, an area highly impacted by human influence. The estimated daily exposure for Brazilian population that consumes the studied species was up to 20.3 ng/kg bw/day via carib pointed-venus and 25.7 ng/kg bw/day via snooks, lower than acceptable daily intake. Thus, consumption of seafood species from Sepetiba Bay and Parnaiba Delta River seems to be safe to the population in what concerns the PhACs studied.


Asunto(s)
Preparaciones Farmacéuticas , Contaminantes Químicos del Agua , Animales , Brasil , Monitoreo del Ambiente , Humanos , Medición de Riesgo , Alimentos Marinos/análisis , Contaminantes Químicos del Agua/análisis
2.
Food Chem ; 371: 131309, 2022 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-34638014

RESUMEN

A novel fluorescent probe bearing triphenylamine, thiophene and thiosemicarbazide (TPA-TSC) was developed, and its distinct features were investigated. TPA-TSC demonstrated excellent selectivity towards Hg2+ ions, with a limit of detection (0.14 nM) among the lowest reported to date. It also exhibited a turn-off response by forming stable complex for a brief period (<30 s). Job plot analysis and high-resolution mass spectrometry (HRMS) revealed that TPA-TSC bound to Hg2+ with a 2:1 stoichiometry in an aqueous environment, remarkably the probe successfully detected Hg2+ ions in seabass, swordfish and different samples of water. In all, TPA-TSC offers a promising method for accurately determining the quality of seafood and water samples polluted with Hg2+.


Asunto(s)
Lubina , Mercurio , Animales , Colorantes Fluorescentes , Iones , Límite de Detección , Alimentos Marinos , Espectrometría de Fluorescencia , Agua
3.
Environ Pollut ; 292(Pt B): 118398, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34695516

RESUMEN

Despite the ubiquity of per- and polyfluorinated alkyl substances (PFAS) in all environmental compartments, little is known about the pollution they cause on the African continent, neither on levels, nor effects. Here we examined the occurrence and levels of 21 legacy and emerging PFAS in 9 marine species (3 fish, 2 crustaceans and 4 mollusks) collected from Bizerte lagoon, Northern Tunisia. Furthermore, assessment of potential human health risks through consumption of contaminated seafood was examined. This is the first study assessing PFAS in Mediterranean coastal areas of North Africa. Twelve out of the 21 targeted PFAS were detected, evidencing the occurrence of PFAS in seafood from North Africa, albeit at low levels. The Æ©21PFAS concentrations in all seafood samples ranged from 0.202 ng g-1 dry weight (dw) to 2.89 ng g-1 dw, with a mean value of 1.10 ± 0.89 ng g-1 dw. The profiles of PFAS varied significantly among different species, which might be related to their different trophic level, protein content, feeding behaviour and metabolism. Generally, current exposure to PFAS through seafood consumption indicates that it should not be of concern to the local consumers, at least for those PFAS for which information is available.


Asunto(s)
Fluorocarburos , Contaminantes Químicos del Agua , Animales , Ecosistema , Monitoreo del Ambiente , Peces , Fluorocarburos/análisis , Humanos , Alimentos Marinos , Contaminantes Químicos del Agua/análisis
4.
Environ Pollut ; 292(Pt B): 118388, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34699922

RESUMEN

Seafood is well recognized as a major source of Long Chain n-3 Polyunsaturated Fatty Acids (LC n-3 PUFA, especially ecosapentaenoic acid, i.e. EPA and docosaheaxaenoic acid, i.e. DHA) and essential trace elements (As, Cu, Fe, Mn, Se, and Zn). It is also a source of non-essential trace elements (Ag, Cd, Hg, Pb) that can be deleterious for health even at low concentrations. Edible parts of sixteen species (fish, cephalopods, crustaceans and bivalves) of great importance in the Pertuis Charentais region, one of the main shellfish farming and fishing areas along the french coastline, were sampled in winter and analyzed to determine their fatty acid (FA) composition and trace element concentrations. Based on these analyses, a suite of indices was calculated to estimate risk and benefit of seafood consumption: the n-6/n-3 ratio, the atherogenic index, the thrombogenic index, the EPA + DHA daily recommended portion, as well as the maximum safe consumption. The results showed that fish contributed the most to LC n-3 PUFA supply, while bivalves and crustaceans were more beneficial in essential trace elements. Whatever the species, the concentrations of non-essential elements were not limiting for seafood consumption, as important amounts of the analyzed species can be eaten daily or weekly before becoming hazardous to consumers. Yet, concentrations of Hg in dogfish and seabass can become a concern for frequent seafood consumers (>three meals a week), confirming that varying seafood items is a key point for consumers to optimize the benefits of diverse seafood resources. Considering FA composition, whiting and pilchard are the most beneficial fish species for human diet, while surmullet was the least beneficial one. However, using an index integrating the relative risk due to Hg content, the surmullet appears as one of the most beneficial. This study provides a temporal shot of the quality of marine resources consumed in winter period in the studied area and highlights the complexity of a quantitative risk and benefit assessment with respect to the biochemical attributes of selected seafood.


Asunto(s)
Mercurio , Oligoelementos , Animales , Peces , Contaminación de Alimentos/análisis , Humanos , Medición de Riesgo , Alimentos Marinos/análisis , Mariscos , Oligoelementos/análisis
5.
Food Chem ; 371: 131133, 2022 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-34808758

RESUMEN

Octopus play an increasingly important role in ocean ecosystems and global fisheries, yet techniques for authenticating provenance are sorely lacking. For the first time, we investigate whether chemical profiling can distinguish geographical origins of octopus on international and domestic scales. Our samples consisted of wild-caught octopus from south-east Asia and southern Australia, regions with high seafood trade. We used a novel combination of stable carbon (δ13C) and oxygen (δ18O) isotope analyses (Isotope-Ratio Mass Spectrometry) of internal calcified structures called statoliths, with elemental analyses (X-Ray Fluorescence using Itrax) of soft-tissue. We found that multivariate profiles exhibited distinctive regional signatures, even across species, with high classification success (∼95%) back to region of origin. This study validates isotopic and multi-elemental profiling as an effective provenance tool for octopus, which could be used to support transparency and accountability of seafood supply chains and thus encourage sustainable use of ocean resources.


Asunto(s)
Octopodiformes , Animales , Isótopos de Carbono/análisis , Ecosistema , Espectrometría de Masas , Isótopos de Nitrógeno/análisis , Alimentos Marinos/análisis
6.
Food Chem ; 371: 131149, 2022 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-34583173

RESUMEN

This work studies the effect of glycerol on the chemical physics of the thermal gelatin of protein from giant squid minced meat. The presence of glycerol induced changes in the nano protein particles (NPP) self-assembled structures. These nanoscale events resulted in dramatic changes on the interactions between proteins when forming gels, with the contribution of ionic interactions increasing by 17% upon gelation, that of hydrogen bonds reducing by 50%, that of hydrophobic interactions decreasing by 45%, and that of disulphide bonding increasing by 18%. Glycerol also induced cluster formation in myofibrillar solutions. As a result, incorporation of glycerol increased springiness, resilience, and adhesiveness of the formed gels by 13%, 25%, and 370% respectively. The heating gelation of myofibrillar proteins was monitored at various temperatures via recording the elastic and storage moduli. Rheology and micro-rheology studies revealed that the presence of glycerol increased G' and G″ of thermally-gelled giant squid meat.


Asunto(s)
Decapodiformes , Glicerol , Animales , Geles , Reología , Alimentos Marinos
7.
Food Chem ; 372: 131242, 2022 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-34818726

RESUMEN

Many proteins are known to be phosphorylated, affecting important regulatory factors of muscle quality in the aquatic animals. The striated and smooth adductor muscles of Yesso scallop Patinopecten yessoensis were used to investigate muscle texture and identify phosphoproteins by histological methods and phosphoproteomic analysis. Our present study reveals that muscle fiber density is in relation to meat texture of the striated and smooth adductor muscles. The phosphoproteomic analysis has identified 764 down-regulated and 569 up-regulated phosphosites on 743 phosphoproteins in the smooth muscle compared to the striated part. The identification of unique phosphorylation sites in glycolytic enzymes may increase the activity of glycolytic enzymes and the rate of glycolysis in the striated adductor muscle. The present findings will provide new evidences on the role of muscle structure and protein phosphorylation in scallop muscle quality and thus help to develop strategies for improving meat quality of scallop products.


Asunto(s)
Pectinidae , Fosfoproteínas , Animales , Músculo Esquelético , Músculo Liso , Fosfoproteínas/genética , Alimentos Marinos
8.
Sci Total Environ ; 805: 150361, 2022 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-34818778

RESUMEN

The use of aqueous film forming foams (AFFFs) as fire retardants is an critical point-source for per- and polyfluoroalkyl substances (PFASs) pollution into the aquatic environment. This study investigated PFASs pollution in the surface waters and biota (shellfish and fish) of Galveston Bay, following AFFFs use to extinguish a petrochemical fire (March 17th to 20th, 2019) of oil storage tanks at the International Terminals Company (ITC) in Deer Park (Houston, TX). The levels of up to twelve EPA priority PFASs were measured in surface waters and biota from March-November 2019. PFASs levels in surface waters showed mean total levels in March and April 2019 to be from 4× to ~300× higher than those measured in the following months. PFOS (perfluorooctanesulfonic acid) was the most abundant homolog measured at ≥66% of total PFASs. Maximal PFOS levels exceeded the State of Texas' water regulatory limit of 0.6 µg L-1 in 3% of the samples analyzed in March and April 2019. PFOS was also the most prominent homolog (≥66% of total PFASs) measured in eastern oysters (Crassostrea virginica), red drum (Sciaenops ocellatus), gafftopsail catfish (Bagre marinus), and spotted seatrout (Cynoscion nebulosus). A statistically significant elevation of PFOS body-burdens was measured in oysters and spotted seatrout in April and May 2019, respectively. A Hazard Ratio calculation for seafood safety suggests an advisory of 1-2 meals per week for gafftopsail catfish and red drum, and 2 meals per week for spotted seatrout to be protective for human exposure to PFOS. The levels in oysters indicated no immediate concerns for the dietary exposure of humans. Our results highlight a need for continual monitoring to assess the long-term fate and seafood advisories for PFASs.


Asunto(s)
Ácidos Alcanesulfónicos , Ciervos , Fluorocarburos , Contaminantes Químicos del Agua , Ácidos Alcanesulfónicos/análisis , Animales , Bahías , Biota , Fluorocarburos/análisis , Humanos , Alimentos Marinos , Mariscos , Agua , Contaminantes Químicos del Agua/análisis
9.
Sci Total Environ ; 804: 150117, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34508938

RESUMEN

Plastic debris into the environment is a growing threat for the ecosystems and human health. The seafood sector is particularly concerned because it generates plastic losses and can be endangered by plastic contamination. Life cycle assessment (LCA) does not properly consider plastic losses and related impacts, which is a problem in order to find relevant mitigation strategies without burden shifting. This work proposes a methodology for quantifying flows of plastics from the life cycle of the seafood products to the environment. It is based on loss rate and final release rate considering a pre-fate approach as proposed by the Plastic Leak Project. They are defined for 5 types of micro and macro plastic losses: lost fishing gears, marine coatings, plastic pellets, tire abrasion and plastic mismanaged at the end-of-life. The methodology is validated with a case study applied to French fish products for which relevant data are available in the Agribalyse 3.0 database. Results show that average plastic losses are from 75 mg to 4345 mg per kg of fish at the consumer, depending on the species and the related fishing method. The main plastic losses come from lost fishing gears (macroplastics) and tire abrasion (microplastics). Results show high variability: when mismanaged, plastic packaging at the end-of-life (macroplastics) is the main loss to the environment. As a next step the methodology is to be applied to other fish or shellfish products, or directly implemented in a life cycle inventory database. Further research should characterize the related impacts to the environment when life cycle impact assessment methodologies will be available, and identify eco-design solutions to decrease the major flows to the environment identified.


Asunto(s)
Plásticos , Contaminantes Químicos del Agua , Animales , Ecosistema , Monitoreo del Ambiente , Productos Pesqueros , Humanos , Estadios del Ciclo de Vida , Alimentos Marinos , Residuos/análisis , Contaminantes Químicos del Agua/análisis
10.
Food Chem ; 368: 130863, 2022 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-34428691

RESUMEN

A novel sensor based on bead-counting of purple sweet potato tapioca pearl for freshness monitoring of shrimp was proposed. The sensor was prepared from commercially available tapioca pearls with purple sweet potato as a natural colorant by using a similar procedure as to prepare "Thai Saku dessert". A novel concept of using five tapioca pearls stacked in a pipette tip was proposed to observe the color change of the pearls by bead counting approach. The color of the tapioca pearl changed from purple to greenish-blue upon the detection of volatile amines and then to green on prolonged exposure to volatile amines. This color change was observed from the first bead and gradually observed on the next beads according to the concept of distance-based colorimetric measurement. This work is the first to demonstrate the use of bead counting as a novel, low-cost sensor technology for estimating the freshness of shrimp.


Asunto(s)
Ipomoea batatas , Manihot , Animales , Antocianinas , Crustáceos , Alimentos Marinos
11.
Food Chem ; 366: 130574, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34303209

RESUMEN

There is growing interest in chitosan-based intelligent packaging films for monitoring food quality. However, practical application of these biopolymeric films has been limited by their poor physical and mechanical attributes. Herein, a versatile colorimetric indicator film was developed based on chitosan (CHI) and broken Riceberry phenolic extract (RPE). The effects of RPE fortification on the microstructure, physical, and functional attributes of the CHI films were comprehensively evaluated. The results revealed that CHI-RPE films exhibited increased hydrophobicity, mechanical resistance, thermal stability, barrier properties, and antioxidant activity compared to plain CHI film. The CHI-RPE films were cytocompatible. Notably, CHI-RPE film also produced intense naked-eye detectable colorimetric response to pH (2-12) variation and volatile ammonia. When enclosed with fresh shrimp, CHI-RPE film changed from orange-red to yellow in response to shrimp spoilage. Thus, CHI-RPE film has high potential for fabricating pragmatic, smart packaging labels for on-site visual detection of freshness in seafood products.


Asunto(s)
Quitosano , Antocianinas , Antioxidantes , Embalaje de Alimentos , Concentración de Iones de Hidrógeno , Extractos Vegetales , Alimentos Marinos/análisis
12.
Food Chem ; 367: 130721, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34371280

RESUMEN

High pressure (HP) is a non-thermal treatment that is generally used to reduce the microbiological contamination of food products, such as Atlantic salmon (Salmo salar). However, HP is known to alter the stability of proteins and can therefore affect the quality of salmon flesh. In this study, the effects of HP treatment for 5 min at 200, 400 and 600 MPa on the structure of Atlantic salmon were investigated. Transversal histological sections revealed a decrease in the fibre size from 200 MPa associated with an expansion of the extracellular spaces. Connective tissue was found to be modified from 400 MPa, resulting in an increase in its surface area. Fourier transform infrared (FT-IR) microspectroscopy revealed a reduction in the α-helix content and an increase in the aggregated ß-sheet structure content with increasing pressure, reflecting a change in the secondary structure of proteins from 200 MPa.


Asunto(s)
Salmo salar , Animales , Músculos , Estructura Secundaria de Proteína , Alimentos Marinos , Espectroscopía Infrarroja por Transformada de Fourier
13.
Food Chem ; 369: 130567, 2022 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-34492611

RESUMEN

Applications of mass spectrometry-based metabolomics in food science have developed fast in the last decade. Sample preparation and data processing are critical in non-target/metabolomic workflows but there is currently no standardized protocol for the development of these methods. The impact of data processing parameters or the inclusion of a different matrix is not often taken into account during the selection of an extraction. Thus, this study aimed to investigate the impact of different extractions, e.g., QuEChERS, and data processing on the determination of malachite green metabolites in two different organisms, brook trout and shrimp. The results obtained confirm the need for a harmonized approach for the validation of non-target workflows, as depending on the comparison criteria, the matrix, the mode of ionization or data processing, a different extraction could be chosen. This study also identified for the first time des-methylated leucomalachite green as another metabolite in the two organisms.


Asunto(s)
Colorantes de Rosanilina , Alimentos Marinos , Animales , Crustáceos , Alimentos Marinos/análisis , Trucha
14.
Talanta ; 237: 122897, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34736713

RESUMEN

The spectroscopic ellipsometry (SE), and attenuated internal reflection spectroscopic ellipsometry (TIRE) are promising methods in label-free biosensing applications. An ellipsometer running under surface plasmon resonance (SPR) conditions has unique advantages over other SPR-based methods in terms of sensitivity and real-time/label-free measurement capability. In this study, both SE and TIRE-based brevetoxin B (BTX) sensors were developed using two anti-BTX aptamers reported before. A new aptamer sequence was also derived from these two antiBTX aptamers using predictive modeling tools and an exclusion method. All three antiBTX aptamers' analytical performances were quite competitive in terms of both detecting range and detection limits. However, the selectivity of the previously reported aptamers against analogs of BTX was poor at low detection ranges, especially for okadaic acid. Furthermore, the selectivity of the derived aptamer was lower than its predecessors. The sensors were capable of detecting BTX in the range of 0.05 nM-1600 nM in the TIRE and 0.5 nM-2000 nM in the SE configuration. The detection limits of the sensors were 1.48 nM (1.32 ng/mL) and 0.80 nM (0.72 ng/mL) for SE and TIRE configurations, respectively. Both configurations have been used successfully to detect BTX standards spiked into real fish and shrimp samples.


Asunto(s)
Aptámeros de Nucleótidos , Técnicas Biosensibles , Animales , Toxinas Marinas , Oxocinas , Alimentos Marinos/análisis , Análisis Espectral , Resonancia por Plasmón de Superficie
15.
J Hazard Mater ; 421: 126708, 2022 01 05.
Artículo en Inglés | MEDLINE | ID: mdl-34352521

RESUMEN

The level and distribution of 16 USEPA Polycyclic aromatic hydrocarbon (PAHs) in aquaculture farmed fish (Mugil cephalus and Oreochromis mossambicus) and shellfish (Corbicula fluminea Formosa and Meretrix lusoria) were determined in Taiwan and then assessed cancer and non-cancer risks for those consuming these kinds of seafood. Results indicated that C. fluminea Formosa accumulated the highest average concentration of total PAHs (43.0 ± 11.3 ng/g wet weight) while M. lusoria contained the lowest concentration (20.0 ± 5.8 ng/g) among all species. The low-molecular-weight PAHs were dominant for both fish and shellfish, which consistent with other studies. Notably, parts of high-molecular-weight PAHs were found in shellfish whereas that was little in fish. The calculated hazard quotients (HQ) of all PAHs were smaller than 1 and the incremental lifetime cancer risks (ILCR) for Benzo[a]pyrene were below 1 × 10-5, suggesting that PAHs in the collected seafood could pose a low hazard to residents. Although the results indicated that the studied seafood is safe for human consumption, children and seniors post relatively higher risks, suggesting that it needs to continue monitoring and control the PAHs concentration in seafood and the associated environments.


Asunto(s)
Hidrocarburos Policíclicos Aromáticos , Animales , Acuicultura , Niño , Monitoreo del Ambiente , Contaminación de Alimentos/análisis , Humanos , Hidrocarburos Policíclicos Aromáticos/análisis , Estanques , Medición de Riesgo , Alimentos Marinos/análisis , Taiwán
16.
Zhonghua Liu Xing Bing Xue Za Zhi ; 42(8): 1360-1364, 2021 Aug 10.
Artículo en Chino | MEDLINE | ID: mdl-34814554

RESUMEN

Objective: To investigate the contamination status of SARS-CoV-2 in imported frozen seafood from a Russia cargo ship in Qingdao and to analyze the risk factors for infection in local stevedores. Methods: The method of "two-stage, full coverage and mixed sampling" was used to collect the seafood packaging samples for the nucleic acid detection of SARS-CoV-2 by real-time fluorescent quantitative RT-PCR. A unified questionnaire was designed to investigate 71 stevedores in two shifts through telephone interview. The stevedores were divided into two groups, with 23 in the shit with two infections was group A and 48 in the shift without infection was group B. Software Epi Info7.2 was used to identify the risk factors for SARS-CoV-2 infections in the stevedores. Results: In the frozen seafood from a Russia cargo ship, the total positive rate of SARS-CoV-2 nucleic acid in the frozen seafood was 11.53% (106/919). The positive rate of SARS-CoV-2 nucleic acid in the frozen seafood unloaded by group A (14.29%,70/490) was significantly higher than that in the frozen seafood unloaded by group B (8.39%,36/429)(χ2=7.79,P=0.01) and the viral loads detected in the frozen seafood unloaded by group A were higher than those detected in the frozen seafood unloaded by group B. The scores of personal protection and behaviors in the stevedores in group A were significantly lower than those in group B (P<0.05), and toilet use, smoking and improper hand washing before meals were the risk factors for the infection. Conclusions: The imported frozen seafood was contaminated by SARS-CoV-2 and the contamination distribution was uneven. Supervision and management of personal occupational protection and behaviors of workers engaged in imported frozen food transportation should be strengthened. It is suggested that a closed-loop monitoring and management system for the whole process of "fishing-transport- loading/unloading" should be established by marine fishery authority.


Asunto(s)
COVID-19 , SARS-CoV-2 , Humanos , Factores de Riesgo , Alimentos Marinos , Navíos
17.
Braz J Biol ; 84: e253613, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34730702

RESUMEN

Soybean meal is an inexpensive plant origin protein which has been used in practical diets as a replacement of animal protein such as fish meal or chicken meal, due to the uneconomical price of animal protein diets. Consequently, a research study was conducted on some commercial species of Indian major carps i.e. Catla (Cattla cattla), Rohu (Labeo rohita) and Mrigala (Cirhinus mrigala) (Hamilton, 1822) to estimate optimum dietary protein requirement of soy bean meal in diet in an intensive polyculture. Three different diets (SBM I, SBM II and SBM III) were formulated by 80%, 50% and 20% replacement of fish meal with soybean meal from a 45% fish meal diet (control).Highest monthly mean weight gain was obtained by SBM II (with 35% CP and about 50% substitution of fish meal), while SBM III (45% Crude Protein and about 20% substitution of fish meal) was stood second. All tested diets respond enormously by producing high yield as compare to control diet, though SBM II generated highest yield among all. On the bases of the following research, it was revealed that the SBM can surrogate even50% fish meal without any augmentation of other amino acids in the diet of Indian major carps.


Asunto(s)
Carpas , Cyprinidae , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Dieta/veterinaria , Alimentos Marinos , Soja
18.
Shokuhin Eiseigaku Zasshi ; 62(5): 139-147, 2021.
Artículo en Japonés | MEDLINE | ID: mdl-34732639

RESUMEN

We have developed a quantitative determination method of the concentration of inorganic arsenic in pet foods using a liquid chromatograph-inductively coupled plasma-mass spectrometer (LC-ICP-MS). After adding 2 w/v% TMAH solution to a sample, inorganic arsenic was extracted by heating and the extract was collected by water. The pH of the solution was adjusted, and injected into a LC-ICP-MS to determine the concentration of inorganic arsenic. LC separation was carried out on an ODS column with 10 mmol/L sodium 1-butanesulfonate, 4 mmol/L malonic acid, 4 mmol/L TMAH and 0.05% methanol solution as a mobile phase. A collaborative study was conducted by nine laboratories using dry and wet-type pet foods, formed jerky, dried jerky and biscuit. Dry-type pet food and dried jerky was added with 2 mg/kg of As (III). Wet-type pet food was added with 0.5 mg/kg of As (III). Formed jerky was added with 1 mg/kg of As (III). Biscuit was added with 0.2 mg/kg of As (III). The mean recoveries, repeatabilities and reproducibilities in the form of relative standard deviation (RSDr and RSDR), and HorRat, were 95.4% to 98.3%, less than 2.9%, less than 9.1%, and 0.22 to 0.51, respectively.


Asunto(s)
Arsénico , Arsenicales , Cromatografía Líquida de Alta Presión , Espectrometría de Masas , Alimentos Marinos/análisis
19.
Environ Sci Process Impacts ; 23(11): 1803-1814, 2021 Nov 17.
Artículo en Inglés | MEDLINE | ID: mdl-34730156

RESUMEN

The origin, tissue concentration, and health risk of polycyclic aromatic hydrocarbons (PAHs) contaminants in three economically important species of seafood, including catfish (Chrysichthys nigrodigitatus), prawns (Macrobrachium macrobrachium), and periwinkles (Tympanotonus fuscatus) from the crude oil-impacted Niger Delta region, were investigated. The concentrations of PAHs were measured by coupled gas chromatography-mass spectrometry after repeated extraction by ultrasonication in hexane and cleaning up in silica gel. The origin of PAHs was deduced using established mathematical protocols. Health risk from the consumption of contaminated seafood was evaluated for 60 kg bodyweight individuals at a fish consumption rate of 36.94 g per person per day. Different tissue concentrations of PAHs residues at low, moderate, and chronic levels were revealed. Mean total PAHs varied from 4.55 to 6.36 mg kg-1 in catfish, 4.61 to 7.75 mg kg-1 in prawns, and 4.91 to 6.14 mg kg-1 in periwinkles. The tissue concentrations were high above PM2.5, enough to suspect PAHs-related health risk, especially among residents who consume a large quantity of seafood. Carcinogenic PAHs index, benzo[a]pyrene, varied from below instrument detection (<0.01) to 0.29 mg kg-1. The estimated carcinogenic potency equivalent concentrations (PEC) of PAH varied from 0.653 to 2.153 above the screening value (SV), 0.01624 in the three species investigated. Mathematical evaluation and dominant tissue concentration of high molecular weight PAHs in all the seafood investigated showed pyrogenic origin of PAHs.


Asunto(s)
Hidrocarburos Policíclicos Aromáticos , Animales , Monitoreo del Ambiente , Humanos , Niger , Nigeria , Hidrocarburos Policíclicos Aromáticos/análisis , Medición de Riesgo , Alimentos Marinos/análisis
20.
Compr Rev Food Sci Food Saf ; 20(6): 5421-5448, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34633147

RESUMEN

Cell-cultured meat and seafood offer a sustainable opportunity to meet the world's increasing demand for protein in a climate-changed world. A responsible, data-driven approach to assess and demonstrate safety of cell-cultured meat and seafood can support consumer acceptance and help fully realize the potential of these products. As an initial step toward a thorough demonstration of safety, this review identifies hazards that could be introduced during manufacturing, evaluates applicability of existing safety assessment approaches, and highlights research priorities that could support safe commercialization. Input was gathered from members of the cultured meat and seafood industry, researchers, regulators, and food safety experts. A series of workshops were held with 87 industry representatives and researchers to create a modular manufacturing process diagram, which served as a framework to identify potential chemical and biological hazards along the steps of the manufacturing process that could affect the safety of a final food product. Interviews and feedback on draft documents validated the process diagram and supported hazard identification and evaluation of applicable safety methods. Most hazards are not expected to be novel; therefore, safety assessment methods from a range of fields, such as conventional and novel foods, foods produced from biotechnology, pharmaceuticals, and so forth, are likely to be applicable. However, additional assessment of novel inputs or products with significant differences from existing foods may be necessary. Further research on the safety of the inputs and associated residues, potential for contamination, and development of standardized safety assessment approaches (particularly animal-free methods) is recommended.


Asunto(s)
Inocuidad de los Alimentos , Alimentos Marinos , Comercio , Carne , Investigación , Alimentos Marinos/análisis
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