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1.
Methods Mol Biol ; 2259: 215-223, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33687718

RESUMEN

A workflow for the characterization of food-derived bioactive peptides is described in this chapter. The workflow integrates two consecutive steps: a discovery phase and a protein-based bioinformatic phase. In the first step (discovery phase), a shotgun bottom-up proteomics approach is used to create a reference data set for a selected food proteome. Afterward, in a second step (bioinformatic phase), the reference proteome is subjected to several in silico protein-based bioinformatic analyses to predict and characterize potential bioactive peptides after an in silico human gastrointestinal digestion. Using this workflow, bioactive collagen peptides, antihypertensive, antimicrobial, and antitumor peptides were predicted as potential valuable bioactive peptides from seafood and marine by-products. It is concluded that the combination of the global shotgun proteomic analysis and the analysis by protein-based bioinformatics can provide a rapid strategy for the characterization of new potential food-derived bioactive peptides.


Asunto(s)
Proteínas en la Dieta/análisis , Péptidos/análisis , Proteómica/métodos , Cromatografía Liquida/métodos , Alimentos Funcionales/análisis , Humanos , Alimentos Marinos/análisis , Espectrometría de Masas en Tándem/métodos
2.
Food Chem ; 352: 129334, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33657479

RESUMEN

Canned fish is submitted to processes that may degrade its lipids and form harmful compounds called cholesterol oxidation products (COPs). Samples of Brazilian commercial canned tuna were analyzed to evaluate the influence of different liquid mediums (oil and brine) on the fatty acid composition and formation of COPs. The exchange between fish lipids and the constituents of the covering liquid was highlighted by the high levels of linoleic acid found in tuna conserved in oil. High amounts of COPs were found. However, higher contents of COPs were found in tuna in brine (933.14 to 1914.23 µg/g) than in oil (698.24 to 1167.88 µg/g). This result was mainly promoted by the presence of pro-oxidant elements such as salt, as well as greater heat transfer in brine than in oil. This study showed that canned tuna is a potential source of exogenous COPs, indicating the role of liquid mediums in oxidative processes.


Asunto(s)
Colesterol/química , Lípidos/análisis , Atún/metabolismo , Animales , Ácidos Grasos/análisis , Calor , Oxidación-Reducción , Alimentos Marinos/análisis
3.
Food Chem ; 351: 129347, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33647702

RESUMEN

In this study, two active packaging types were produced: single-layer biopolymer films with a polysaccharide - furcellaran and carp skin gelatin hydrolysate; two-layer films with identical composition, but synthetic peptide Alanina-Tyrosine addition. The procedure objective was multiplying antioxidant effects of the hydrolysate complexed with furcellaran. Films were used on Atlantic mackerel (storage 4 °C, 15 days); samples were analysed for changes in microbiological quality, TVB-N, biogenic amine content, fatty acid composition, TBARS. Consumer analysis was performed characterising mackerel carcass perception depending on implemented active coatings. The developed innovative single- and double-layer coatings effectively slow down lipid oxidation processes, especially at the initial period of Atlantic mackerel storage in refrigerated conditions. The coatings effectively inhibited microorganism growth, extending shelf-life by 2 days, single-layer coatings showing greater efficiency. According to consumers, coating application did not adversely affect product attractiveness parameters. The developed innovative coatings show great applicative potential as a new active packaging for perishable foods.


Asunto(s)
Alginatos/química , Antioxidantes/química , Carpas/anatomía & histología , Embalaje de Alimentos/métodos , Gelatina/química , Péptidos/química , Gomas de Plantas/química , Piel/química , Animales , Hidrólisis , Oxidación-Reducción , Perciformes , Alimentos Marinos/análisis
4.
Food Chem ; 351: 129296, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33640769

RESUMEN

This study evaluated the effects of treatments of squid pen chitooligosaccharide (COS) or epigallocatechin gallate (EGCG) or COS/EGCG mixture (1:1, w/w) at different concentrations (0, 200, and 400 mg/kg) on the discoloration and quality changes in yellowfin tuna slices stored at 4 °C for 12 days. Tuna slices added with 200 and 400 mg/kg of COS (C2 and C4, respectively) showed the lowest reduction in oxymyoglobin and a* value (redness) ascertained by the lower metmyoglobin formation than other samples. Additionally, C2 and C4 samples showed a lower total viable count and TBARS value than the remaining samples. EGCG alone and its mixture with COS exhibited lower efficacy in retaining the quality loss than COS alone. COS at both levels effectively reduced the metMb formation. It maintained the redness with sensory acceptability of slices up to 9 days, and C4 sample prolonged shelf-life for 12 days based on the microbiological limit.


Asunto(s)
Catequina/análogos & derivados , Quitina/análogos & derivados , Metamioglobina/química , Mioglobina/química , Alimentos Marinos/análisis , Atún , Animales , Catequina/química , Quitina/química , Color , Decapodiformes/química
5.
Mar Pollut Bull ; 165: 112073, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33549996

RESUMEN

210Po concentration in thirty popular marine species (twenty five fishes, four bivalves and one crab) with different habitats, feeding types, and trophic levels at the near-shore Binh Thuan, Vietnam was analyzed using the alpha detectors. The 210Po activities were widely ranged from 3.07 ± 0.18 in a fish species (Bumpnose trevally) to 78.6 ± 5.3 Bq/kg w.w in a bivalve species (Green mussel) with the average value of 16.6 Bq/kg w.w. The results showed that the species with lower trophic level value have higher 210Po concentration. Regarding the feeding types, the 210Po concentration in the omnivorous species is about 2.9 times higher than in the carnivorous species. The species living in demersal zone accumulated a high 210Po in comparison with species living in reef-associated and pelagic-neritic zones. The calculated annual committed effective dose from 210Po due to the ingestion of seafood from demersal zone and bivalve species of the area was significant high.


Asunto(s)
Polonio , Contaminantes Radiactivos del Agua , Animales , Peces , Polonio/análisis , Alimentos Marinos/análisis , Vietnam , Contaminantes Radiactivos del Agua/análisis
6.
Mar Pollut Bull ; 165: 112108, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33581567

RESUMEN

In this study, metal compositions in anchovy (Engraulis encrasicolus) sampled from 11 different sites representing Turkey, Georgia, and Abkhazia coasts of the Black Sea were investigated. For this purpose, micro (Al, Zn, Mn, Co, Cr, Cu, Fe, Ni, Cd, Pb, Se, As, and Hg) and macro (K, Ca, Na, Mg, P) element content in edible muscle tissue of anchovy were determined. In addition, the potential risks associated with human consumption of the samples were evaluated using quality indices such as estimated weekly intake (EWI), target hazard quotient (THQ), and total exposure hazard index (HI). The results showed that the potassium (K) concentration was the highest in edible tissue of the anchovies from all stations. Anchovies were also found to be rich in phosphorus and calcium. When the metal content of anchovies was compared, there were statistically variations among metal concentrations (except for Co, Ni, Cu, Cr, Cd, Pb, and Hg) in the muscle tissue of anchovies according to the stations (P<0.05). The concentrations of Pb, Cd, Zn, Cu, Ni, and Cr in anchovy were found below the maximum permissible values determined by various national and international organizations for seafood. Besides, when the samples were examined in terms of EWI, THQ, and HI quality indices, it was determined that anchovy consumption did not pose a potential hazard to human health for the consumption of the anchovy. The present study conclusively indicated that no health problem can be raised from human consumption of the examined commercial anchovy along the Turkey, Georgia, and Abkhazia coasts of the Black Sea.


Asunto(s)
Metales Pesados , Animales , Mar Negro , Monitoreo del Ambiente , Georgia , Humanos , Metales Pesados/análisis , Medición de Riesgo , Alimentos Marinos/análisis , Turquia
7.
Environ Pollut ; 276: 116686, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33611198

RESUMEN

Previous studies have shown that accumulation of perfluoroalkyl acids (PFAAs) in the tissues of aquatic species is highly variable. Movement and migration patterns in these species represent an important consideration when evaluating contaminant accumulation in exposed biota, and may have a large influence on the risk profiles for migratory seafood species. In this study, relationships between PFAA concentrations in muscle and liver tissue, and recent fish migration history (inferred from metals profiles in fish otoliths, otherwise known as otolith chemistry) were evaluated in Sea Mullet (Mugil cephalus). A greater number of PFAAs, and higher concentrations, were found in liver compared to muscle tissue. Perfluorooctane sulfonate (PFOS) was present in highest concentrations in both muscle and liver tissues, and there was strong correlation in concentrations between these two tissues. PFOS was found to decrease and increase alongside recent strontium and barium concentrations (respectively) in the otolith, suggesting higher concentrations of PFAAs in fish recently exposed to comparatively lower salinity environments. This study highlights how otolith chemistry can be employed to examine links between contaminant concentrations in fish, and their recent migration history. This approach shows promise for studying contaminant residues in mobile seafood species within the natural environment.


Asunto(s)
Ácidos Alcanesulfónicos , Fluorocarburos , Smegmamorpha , Contaminantes Químicos del Agua , Animales , Peces , Fluorocarburos/análisis , Alimentos Marinos/análisis , Contaminantes Químicos del Agua/análisis
8.
Environ Pollut ; 276: 116684, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33618116

RESUMEN

Seafood contamination with, and human consumption of, microplastics (MPs) have recently been highlighted as an emerging concern for global food security. While there is evidence that commercial marine species are contaminated with MPs, it is still unknown if seafood can act as a vector for MP transfer to human consumers. Microplastics have been reported in the digestive tract, gills and in select internal organs of marine animals. However, many of these tissues are not typically eaten by human consumers but discarded. In this critical review, we examined the peer-reviewed literature for evidence of MP contamination in seafood, and the potential transfer to human consumers. Based on known seafood consumption patterns in a typical Australian diet, we assessed the relevance and reliability of the current body of literature to examine the prospect and risk of MP transfer. The relevance of data was considered based on the organism studied, origin of the samples, and the tissues analysed, while reliability was assessed based on procedural methodologies used to derive the data. A review of 132 studies found limited evidence of MP contamination in edible tissues from fresh fish or crustaceans. MP presence was confirmed in packaged fish, as well as in fresh and packaged bivalve molluscs. The limited number of studies satisfying the relevance and reliability criteria (n = 24) precluded a quantitative assessment of the potential risk associated with MP transfer. While consumption of packaged fish and bivalve molluscs may result in the consumption of MPs by humans, it is currently unknown whether this presents a health risk.


Asunto(s)
Microplásticos , Contaminantes Químicos del Agua , Animales , Australia , Monitoreo del Ambiente , Contaminación de Alimentos/análisis , Humanos , Plásticos , Reproducibilidad de los Resultados , Alimentos Marinos/análisis , Contaminantes Químicos del Agua/análisis
9.
Food Chem ; 350: 129214, 2021 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-33601093

RESUMEN

Tetrabromobisphenol A (TBBPA), halogenated carbazole (HCZ), and their analogs are the emerging pollutants invading the marine environment. So far, a few methods have been reported for the simultaneous analysis of these pollutants due to their large polarity difference. In this study, an effective extraction and cleanup strategy was developed for the simultaneous determination of 19 TBBPA and HCZ congeners in seafood. The 19 analytes could be directly analyzed through high performance liquid chromatography after ultrasonic extraction (methanol, duplicate ethyl acetate-acetone (1:1, v/v)) and gel permeation chromatography cleanup. The acceptable spike-recoveries were within 65.7-118.3%; the precision was intra-/inter-day RSDs: 0.0-6.7%/0.0-8.5%; and the matrix effects were between -14.1% and 12.4%. The detection limits and quantification limits were 0.002-0.014 and 0.020-0.200 µg g-1 dw, respectively. Additionally, this method successfully analyzed the seafood samples and the concentrations of these analytes were in range of nd-5.4 µg g-1 dw.


Asunto(s)
Carbazoles/análisis , Fraccionamiento Químico/métodos , Análisis de los Alimentos/métodos , Halogenación , Bifenilos Polibrominados/análisis , Bifenilos Polibrominados/aislamiento & purificación , Alimentos Marinos/análisis , Carbazoles/química , Contaminantes Ambientales/análisis , Contaminantes Ambientales/química , Contaminación de Alimentos , Factores de Tiempo
10.
Food Chem ; 345: 128781, 2021 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-33601653

RESUMEN

A new iron-magnetic nanomaterial functionalized with organophosphorus compound was used as solid-phase for arsenic speciation analysis in seafood samples by ICP-MS. The procedure was optimized using chemometric tools and the variables pH = 4.0, 15 min extraction time, and 20 mg of mass of material were obtained as the optimum point. The inorganic arsenic (iAs) extracted using nanoparticles presented concentrations between 20 and 100 µg kg-1 in the evaluated samples. The method was validated for accuracy using CRMs DOLT-5 and DORM-4. It was possible to reuse the same magnetic nanomaterial for 6 successive cycles, and we obtained a detection limit of 16.4 ng kg-1. The proposed method is suitable for the use of inorganic speciation of As, presenting good accuracy, precision, relatively low cost, and acquittance to green chemistry principles.


Asunto(s)
Arsenicales/análisis , Nanopartículas de Magnetita/química , Espectrometría de Masas , Penaeidae/química , Animales , Arsenicales/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Óxido Ferrosoférrico/química , Límite de Detección , Penaeidae/metabolismo , Alimentos Marinos/análisis
11.
Food Chem ; 345: 128812, 2021 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-33601655

RESUMEN

Due to complex matrixes and specific reagent deficiency, the rapid detection of histamine is still a challenge to date. Based on the high peroxidase-like activity of iron-cobalt co-doped carbon dots, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established for histamine detection using the mimic enzyme labeled with histamine antibody (His-Ab). Through the competitive binding of the labeled His-Ab to solid-phase and sample antigens, histamine content was detected with a linear range of 2.5-150 µg mL-1. The detection limit based on 3σ/K was 0.50 mg kg-1, which was much lower than those of commercial His-kit and HPLC methods. The ic-ELISA method was applied to histamine detection in fish samples with the recovery of (103.4 ± 0.5)%, which was in accord with those of commercial His-kit and HPLC methods. The results indicated that the established ic-ELISA method was suitable for rapid detection of histamine in fish samples with high accuracy, sensitivity and stability.


Asunto(s)
Peces/metabolismo , Histamina/análisis , Puntos Cuánticos/química , Animales , Anticuerpos/química , Anticuerpos/inmunología , Carbono/química , Cobalto/química , Ensayo de Inmunoadsorción Enzimática , Histamina/inmunología , Hierro/química , Límite de Detección , Reproducibilidad de los Resultados , Alimentos Marinos/análisis
12.
Food Chem ; 350: 129196, 2021 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-33607409

RESUMEN

Histamine poisoning from seafood is a significant public health and safety concern. To detect histamine sensitively and accurately, a novel competitive sandwich immunoassay using a modified biotin-streptavidin system coupling with polylysine was developed. Using this strategy, a sandwich ELISA with an IC50 value of 112.8 ng mL-1 and a broad linear range of 11.7-1500 ng mL-1 with a correlation coefficient of 0.9942 was validated. Without any sample derivatization procedure, the recovery of histamine ranged from 80.19% to 108.3% with a coefficient of variation of 1.43-11.7% in tuna, prawn and crab. The sandwich ELISA had a detectionlimit of 5.86 ng mL-1, which was 15-fold lower than an indirect competitive ELISA (ic-ELISA). This simple, sensitive and accurate method can be applied to detect histamine in routine seafood samples.


Asunto(s)
Biotina/metabolismo , Braquiuros/química , Decápodos/química , Ensayo de Inmunoadsorción Enzimática/métodos , Histamina/análisis , Estreptavidina/metabolismo , Atún , Animales , Análisis de los Alimentos , Límite de Detección , Alimentos Marinos/análisis
13.
Ecotoxicol Environ Saf ; 213: 112038, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33636467

RESUMEN

Mercury (Hg2+) and benzo[a]pyrene (BaP) are ubiquitous and persistent pollutants with multiple toxicities in bivalve molluscs. Here, the toxicological responses in the gills of Manila clams, Ruditapes philippinarum, to Hg2+ (10 µg L-1), BaP (3 µg L-1), and their mixture were analysed using transcriptomics and metabolomics approaches. Comparisons of the transcriptomes and metabolomes of Hg2+-and/or BaP-treated clams with control animals revealed the involvement of the detoxification metabolism, immune defence, energy-related pathways, and osmotic regulation in the stress response of R. philippinarum. Exposure to Hg2+ alone primarily enhanced the detoxification and energy metabolic pathways by significantly increasing the expression of genes associated with heat-shock proteins and oxidative phosphorylation. However, co-exposure to Hg2+ and BaP caused greater immunotoxicity and disrupted detoxification metabolism, the TCA cycle, glycolysis, and ATP generation. The expression levels of cytochrome P450 1A1 (CYP1A1), multidrug resistance-associated protein 1 (MRP1), and myosin (MYO), and the activity of electron transport system (ETS) in gills were detected, supporting the underlying toxic mechanisms of Hg2+ and BaP. We suggest that the presence of BaP enhances the toxicity of Hg2+ by 1) hampering the detoxification of Hg2+, 2) increasing the immunotoxicity of Hg2+, and 3) constraining energy availability for clams.


Asunto(s)
Benzo(a)pireno/toxicidad , Bivalvos/fisiología , Mercurio/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Bivalvos/metabolismo , Biología Computacional , Branquias/metabolismo , Mercurio/análisis , Metabolómica , Alimentos Marinos/análisis , Transcriptoma/fisiología
14.
Ecotoxicol Environ Saf ; 208: 111765, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396084

RESUMEN

Recent studies have shown that organisms including humans are exposed to microplastics directly or indirectly. The present study aims to examine the ingestion of these microplastics and the consequences of the same by studying the accumulation behavior of weathered Polyethylene (wPE) microplastics. The Perna viridis were exposed chronically to three different environmentally relevant concentrations of wPE for 30 days, followed by a one-week depuration phase. There was no mortality observed in the control and exposed groups, but the feeding rate was observed to have substantially decreased in the group exposed to higher concentration (3 µgL-1) of wPE. It was also observed that a higher number of wPE particles accumulated in the intestine of exposed organisms. Interestingly, the present study revealed the presence of the substantial number of wPE particles in exposed organisms, which may adversely affect the internal organs as well as growth and reproduction. This study perceived that accumulation is marginally influenced by size of wPE. Similarly, biomarker analysis showed that wPE exposure significantly altered both the metabolism and histology of the internal organs of the exposed organisms. Overall, the study confirmed that the intestine was the most sensitive organ followed by gills, adductor muscles, and foot tissue adding new insights into the adverse effects of wPE in the marine ecosystem.


Asunto(s)
Microplásticos/toxicidad , Perna/fisiología , Polietileno/metabolismo , Contaminantes Químicos del Agua/metabolismo , Animales , Ecosistema , Ecotoxicología , Branquias/efectos de los fármacos , Humanos , Microplásticos/metabolismo , Perna/efectos de los fármacos , Plásticos , Polietileno/toxicidad , Alimentos Marinos/análisis , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad
15.
Ecotoxicol Environ Saf ; 208: 111768, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396086

RESUMEN

Scallops are one of the most common bivalves, large-scale farmed in the coastal areas of China. Three species of scallops (Chlamys farreri, Argopecten irradians and Patinopecten yessoensis) from 10 samples sites along the Bohai Sea and the Yellow Sea were collected to investigate species-specific and tissues-specific bioaccumulation, spatial-temporal distribution and health risks for people. Cd must be paid attention since Cd concentrations in 96% of scallop samples exceeded standard with the highest Cd bioaccumulation potentials (BCF) >10,000 while those of the other metals were less than the corresponding limits except Zn with exceeding-limit percentage of 13%. The metal pollution index values showed that A. irradians could accumulate more metals than the other two species, and scallops in the Bohai Sea were polluted more seriously by heavy metals than in the Yellow Sea. The capacities of tissues in scallops to accumulate metals generally followed the order of digestive gland > gill > adductor muscle. However, Zn accumulated in gill was more than that in digestive gland and muscle. Adults and children would face the non-cancer risks because of the accumulation Cd in scallops based on health risk assessment. Cd was the major contributor of health risk to account for 85% of total hazard quotient and 48% of total cancer risk. Scallops could accumulate Cd rapidly from ambient environments to cause health risks according to the transplantation test. Moreover, the recommended maximum edible amounts of whole scallops were 127/63 g/week for adult/children on the basis of the provisional tolerance weekly intake. Humans are recommended to only consume adductor muscles for reducing health risks.


Asunto(s)
Acuicultura , Cadmio/análisis , Exposición Dietética/estadística & datos numéricos , Metales Pesados/análisis , Pectinidae , Contaminantes Químicos del Agua/análisis , Adulto , Animales , Bivalvos , Niño , China , Monitoreo del Ambiente , Contaminación Ambiental , Humanos , Medición de Riesgo , Alimentos Marinos/análisis , Especificidad de la Especie , Agua
16.
Ecotoxicol Environ Saf ; 208: 111589, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396112

RESUMEN

Marine bivalves have been widely applied as environmental contamination bioindicators, although studies concerning tropical species are less available compared to temperate climate species. Assessments regarding Perna perna mytilid mussels, in particular, are scarce, even though this is an extremely important species in economic terms in tropical countries, such as Brazil. To this end, Perna perna mytilids were sampled from two tropical bays in Southeastern Brazil, one anthropogenically impacted and one previously considered a reference site for metal contamination. Gill metallothionein (MT), reduced glutathione (GSH), carboxylesterase (CarbE) and lipid peroxidation (LPO) were determined by UV-vis spectrophotometry, and metal and metalloid contents were determined by inductively coupled plasma mass spectrometry (ICP-MS). Metalloprotein metal detoxification routes in heat-stable cellular gill fractions were assessed by size exclusion high performance chromatography (SEC-HPLC) coupled to an ICP-MS. Several associations between metals and oxidative stress endpoints were observed at all four sampling sites through a Principal Component Analysis. As, Cd, Ni and Se contents, in particular, seem to directly affect CarbE activity. MT is implicated in playing a dual role in both metal detoxification and radical oxygen species scavenging. Differential SEC-HPLC-ICP-MS metal-binding profiles, and, thus, detoxification mechanisms, were observed, with probable As-, Cu- and Ni-GSH complexation and binding to low molecular weight proteins. Perna perna mussels were proven adequate tropical bioindicators, and further monitoring efforts are recommended, due to lack of data regarding biochemical metal effects in tropical species. Integrated assessments, as performed herein demonstrate, are invaluable in evaluating contaminated aquatic environments, resulting in more accurate ecological risk assessments.


Asunto(s)
Metales/toxicidad , Perna/fisiología , Contaminantes Químicos del Agua/toxicidad , Animales , Bahías , Brasil , Monitoreo del Ambiente , Branquias/efectos de los fármacos , Glutatión/metabolismo , Metaloproteínas/metabolismo , Metalotioneína/metabolismo , Metales/análisis , Metales/metabolismo , Perna/efectos de los fármacos , Alimentos Marinos/análisis , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/metabolismo
17.
Environ Monit Assess ; 193(2): 78, 2021 Jan 21.
Artículo en Inglés | MEDLINE | ID: mdl-33474598

RESUMEN

Illegal usage of formaldehyde and ammonia by fishermen and fish sellers for longer preservation of fish and other seafoods during sales and transportation has recently become a problem. Therefore, the present study was conducted to verify the presence or absence of formaldehyde and ammonia in fish and other seafoods that are commercially important for India, which are obtained from fish markets in Chennai. The present investigation results reveal contamination with formaldehyde and ammonia of commercially important fish and other seafoods collected from fish markets in Chennai. Formaldehyde was detected in 100% of samples in N4 Beach fish market, 93.33% of samples in Chintadripet fish market, 80.77% of samples in Vanagaram fish market, 68% of samples in Pattinapakkam fish market, and 58.33% of samples in Kasimedu fish market. Ammonia was detected in 100% of fish samples in N4 Beach, Chintadripet, Pattinapakkam, and Kasimedu fish market. Since formaldehyde and ammonia are harmful to human health, there is a need to develop a more suitable technology to extend the shelf life of the fish and other seafoods during transport and storage for longer preservation. Strict regulations should be made to control the usage of these harmful chemical substances.


Asunto(s)
Amoníaco , Monitoreo del Ambiente , Animales , Formaldehído , Humanos , India , Alimentos Marinos/análisis
18.
Food Chem ; 348: 129129, 2021 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-33515952

RESUMEN

The potential of two different hyperspectral imaging systems (visible near infrared spectroscopy (Vis-NIR) and NIR) was investigated to determine TVB-N contents in tilapia fillets during cold storage. With Vis-NIR and NIR data, calibration models were established between the average spectra of tilapia fillets in the hyperspectral image and their corresponding TVB-N contents and optimized with various variable selection and data fusion methods. Superior models were obtained with variable selection methods based on low-level fusion data when compared with the corresponding methods based on single data blocks. Mid-level fusion data achieved the best model based on CARS, in comparison with all others. Finally, the respective optimized models of single Vis-NIR and NIR data were employed to visualize TVB-N contents distribution in tilapia fillets. In general, the results showed the great feasibility of hyperspectral imaging in combination with data fusion analysis in the nondestructive evaluation of tilapia fillet freshness.


Asunto(s)
/métodos , Alimentos Marinos/análisis , Animales , Procesamiento de Imagen Asistido por Computador , Espectroscopía Infrarroja Corta , Tilapia/metabolismo
19.
Food Chem ; 348: 129134, 2021 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-33516993

RESUMEN

In this study, similarities and differences of sodium tripolyphosphate (STPP) and sodium trimetaphosphate (STMP) pre-soaking on the stability of muscle proteins in shrimp were investigated during 12 weeks of frozen storage (-30 °C). The physicochemical analysis indicated significant improvements in the WHC, springiness, chewiness, and thermal stability of STPP and STMP pre-soaked samples when compared to the control. Interestingly, STMP pre-soaking showed better cryoprotective effects than the STPP treatment when the storage period reached the end of the 12 weeks. Furthermore, the label-free based proteomics results indicated that 62 upregulated differentially abundant proteins (DAPs) were detected in STMP when compared to STPP. These identified DAPs specifically included 40S ribosomal proteins, actin-related proteins, heat shock proteins, myosin heavy chain, and tubulin beta chain. Additionally, the gene ontology (GO) and eukaryotic clusters of orthologous group (KOG) analyses verified that the incorporation of STMP molecules enhanced the resistance of cytoskeleton proteins to cold-temperature stress.


Asunto(s)
Almacenamiento de Alimentos/métodos , Penaeidae/metabolismo , Polifosfatos/química , Alimentos Marinos/análisis , Animales , Cromatografía Líquida de Alta Presión , Congelación , Proteínas de Choque Térmico/metabolismo , Penaeidae/química , Péptidos/análisis , Proteínas de Mariscos/metabolismo , Espectrometría de Masas en Tándem , Factores de Tiempo
20.
J Food Sci ; 86(2): 376-384, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33438246

RESUMEN

Water and texture properties are important indicators for evaluating surimi and surimi seafood quality. The present study assessed the impact of surimi processing on the water and texture properties and analyzed their correlations. The results showed that the moisture content exhibited significant positive correlations with T22 and A22 (P < 0.05). However, water-holding capacity (WHC) revealed significant negative correlation with T21 and A22 (P < 0.05). Moreover, correlations between water and texture properties were observed. Moisture content and A22 displayed significant negative correlation with hardness (P < 0.05). WHC exhibited significant positive correlations with cohesiveness, springiness, and chewiness (P < 0.05), whereas T21 exhibited significant negative correlation with those properties (P < 0.05). Springiness is an important indicator for texture properties of surimi gel. T21 and WHC exhibited significant correlations with springiness (P < 0.05). We used two equations to assess the relationship between springiness and T21 (y = 9.64 × x2 - 33.79 × x + 29.62, R2 = 0.995, P < 0.05) and between springiness and WHC (y = 0.57 + 9.05/{1+e[-29.29 × ( x - 83.90)] }0.01 , R2 = 0.999, P < 0.05), respectively. Verification experiments proved that these two regression equations could be used to predict the change law among feature indicators during the processing of surimi seafood. The present study finds an easy-to-control method to monitor the quality of surimi production and processing for all the stages, and provides ideas for quality design of surimi products. PRACTICAL APPLICATION: The quality of surimi seafood is affected by the process used for surimi production. Early detection of the material quality can efficiently prevent resource wastage. The feature indicators and their correlations help in easy-to-control of all surimi processing stages. Low-field nuclear magnetic resonance (LF-NMR) is a fast, nondestructive method for monitoring food real-time, but the instrument is expensive; whereas water-holding capacity and texture profile analysis (TPA) are traditional methods but time-consuming and material-consuming. Surimi enterprises or researchers choose the right methods based on their needs.


Asunto(s)
Peces , Manipulación de Alimentos/métodos , Alimentos Marinos/análisis , Agua/análisis , Animales , Dureza , Sensación
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