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1.
Braz Oral Res ; 33: e121, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31939500

RESUMEN

The objective of the study was to analyze the surface area (SA) of the wear caused by simulated chewing on human enamel and opposing restorative material, namely: composite resin (CR), porcelain fused to metal (PFM), lithium disilicate (LD), or monolithic zirconia (MZr). Forty-eight premolars were selected as enamel specimens and divided randomly into 4 groups (n = 48; n =12) used as antagonists in chewing simulation (250,000 loading cycles) against one of the four selected test materials. Enamel and material specimens were scanned and evaluated under digital microscope, and wear SA (mm2) were recorded. Descriptive statistics, paired t-test, one-way ANOVA, and post-hoc Tukey-HSD tests were used for statistics (p < 0.05). The smallest and largest SA were exhibited by enamel against LD (0.80 mm2) and PFM (1.74 mm2), respectively. PFM (3.48 mm2) showed the largest SA and CR (2.28 mm2) showed the smallest SA. Paired t-test for SA values showed significant difference (p < 0.05) in all wear comparisons between materials and enamel antagonists. The wear of materials were greater than that of their respective enamel antagonists (p < 0.05). One-way ANOVA of the logarithmic means of wear SA revealed significant differences (P<0.05). Post-hoc Tukey test revealed significance for PFM (p < 0.05) with other materials. Wear of all test materials was greater compared to the wear of enamel antagonists. PFM and LD caused the largest and the smallest enamel wear, respectively. CR, LD, and MZr are more resistant than PFM to wear after simulated chewing against enamel.


Asunto(s)
Resinas Compuestas/química , Esmalte Dental/química , Porcelana Dental/química , Masticación , Aleaciones de Cerámica y Metal/química , Desgaste de los Dientes/etiología , Circonio/química , Adolescente , Adulto , Análisis de Varianza , Pulido Dental/métodos , Humanos , Procesamiento de Imagen Asistida por Computador , Ensayo de Materiales , Microscopía/instrumentación , Valores de Referencia , Estadísticas no Paramétricas , Propiedades de Superficie , Adulto Joven
2.
J Photochem Photobiol B ; 202: 111724, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31785446

RESUMEN

Laser induced autofluorescence (LIAF) lifetime is useful to distinguish between normal laryngeal tissues and squamous cell carcinoma (SCC) based on variations of their biochemical composition and structure alterations. LIAF was collected from samples constituted by pairs of normal and malignant tissue, which were excised from three patients. Exclusion criteria for samples harvest were: (i) macroscopic changes of normal vocal cord observed during surgery; (ii) previous surgical intervention on vocal cord, (iii) patients treated only with chemotherapy or radiotherapy for carcinoma. Inclusion conditions: men, aged 57-68, non-smokers. A pulsed laser diode excited LIAF at 375 nm and 31 MHz repetition rate; beam full-time width at half-maximum was 87 ps at an average power of 0.49 mW. Mean LIAF lifetime for normal tissues was (3.75 ± 0.49) ns and for malignant (4.37 ± 0.85) ns: it is longer in malignant than in normal tissue. Variance analysis made with Fisher's test has shown no significant difference between patients for normal tissues; the same was true for malignant. Though, when malignant tissue was compared to normal for the same patients as well as between patients, a significant difference (significance level of 5%) was evidenced. Time-resolved LIAF may allow better differentiation between normal and malignant tissues in patients diagnosed with larynx SCC.


Asunto(s)
Laringe/efectos de la radiación , Rayos Láser , Anciano , Análisis de Varianza , Carcinoma de Células Escamosas/patología , Carcinoma de Células Escamosas/cirugía , Neoplasias de Cabeza y Cuello/patología , Neoplasias de Cabeza y Cuello/cirugía , Humanos , Laringe/química , Mediciones Luminiscentes , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias
3.
J Appl Oral Sci ; 28: e20190005, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31800870

RESUMEN

other: Chitosan is a natural, biocompatible chelating substance with potential for dental use. This study compared the effects of final canal irrigation with chitosan and EDTA on dentin microhardness, sealer dentin tubules penetration capacity, and push-out strength. METHODOLOGY: Fifty canine roots were distributed according to the final irrigation protocol (n=10): G1- 15% EDTA with conventional irrigation; G2- 15% EDTA with Endovac; G3- 0.2% chitosan with conventional irrigation; G4- 0.2% chitosan with Endovac; and G5- without irrigation. Specimens were obturated (AH Plus) and sectioned in 3 slices per root third. The first slice was used for microhardness and sealer penetration assessments under a laser confocal microscope. The second was utilized in a push-out strength test. The third slice was discarded. Data were analyzed using 2-way ANOVA and Tukey's post hoc test (α<0.05). Failure mode was determined at x40 magnification. RESULTS: Microhardness reduction was more significant in groups G2 and G4 (p<0.05). Sealer penetration through dentin was significantly greater in group G2 (p<0.05). There was no significant difference between groups G1, G3, and G4 (p>0.05). In general, all experimental groups presented similar bond resistance (p>0.05) that significantly differed from the control (p<0.001). Mixed type failures were predominant. CONCLUSIONS: In general, 0.2% chitosan and 15% EDTA solutions act in a similar manner with regard to the variables studied. The use of Endovac potentiates the effect of these solutions.


Asunto(s)
Quelantes/química , Quitosano/química , Recubrimiento Dental Adhesivo/métodos , Dentina/efectos de los fármacos , Ácido Edético/química , Irrigantes del Conducto Radicular/química , Análisis de Varianza , Resinas Epoxi/química , Pruebas de Dureza , Humanos , Ensayo de Materiales , Microscopía Confocal , Persona de Mediana Edad , Valores de Referencia , Reproducibilidad de los Resultados , Materiales de Obturación del Conducto Radicular/química , Resistencia al Corte , Propiedades de Superficie/efectos de los fármacos
4.
J Appl Oral Sci ; 28: e20190100, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31800872

RESUMEN

OBJECTIVE: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization. METHODOLOGY: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis. RESULTS: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%). CONCLUSION: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar.


Asunto(s)
Cavidad Pulpar/microbiología , Periodontitis Periapical/terapia , Tratamiento del Conducto Radicular/instrumentación , Terapia por Ultrasonido/instrumentación , Adolescente , Adulto , Análisis de Varianza , Bacterias/aislamiento & purificación , Carga Bacteriana , Recuento de Colonia Microbiana , Tomografía Computarizada de Haz Cónico , Sondas de ADN , Femenino , Humanos , Modelos Lineales , Masculino , Persona de Mediana Edad , Irrigantes del Conducto Radicular/uso terapéutico , Tratamiento del Conducto Radicular/métodos , Hipoclorito de Sodio/uso terapéutico , Irrigación Terapéutica/instrumentación , Irrigación Terapéutica/métodos , Resultado del Tratamiento , Terapia por Ultrasonido/métodos , Adulto Joven
5.
Exp Parasitol ; 208: 107779, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31634474

RESUMEN

Here, we report enhanced the in vitro effect of potassium usnate on coupled adult Schistosoma mansoni worms at different time intervals and concentrations. The evaluated schistosomicidal parameters were the following: motility, mortality, fecundity and integumentary changes, as viewed in photomicrographs. Potassium usnate was able to cause 100 and 50% mortality at 100 and 50 µM concentrations, respectively, after 24 h of exposure, while 25 and 12.5 µM concentrations caused changes in motility at 48 and 72 h, and lethality at 96 and 120 h respectively. Eggs were not detected at any of the concentrations analyzed. Photomicrographs revealed morphological tegument alterations within all periods of observation, such as swelling, blisters, dorsoventral contraction, short and curved worms. In conclusion, our results indicate that potassium usnate represents a possible candidate for a new drug in the control of schistosomiasis.


Asunto(s)
Antihelmínticos/farmacología , Benzofuranos/farmacología , Schistosoma mansoni/efectos de los fármacos , Esquistosomiasis mansoni/prevención & control , Análisis de Varianza , Animales , Antihelmínticos/administración & dosificación , Antihelmínticos/química , Benzofuranos/administración & dosificación , Benzofuranos/química , Relación Dosis-Respuesta a Droga , Femenino , Fertilidad/efectos de los fármacos , Masculino , Ratones , Movimiento/efectos de los fármacos , Fotomicrografía , Schistosoma mansoni/fisiología , Esquistosomiasis mansoni/tratamiento farmacológico , Factores de Tiempo
6.
Exp Parasitol ; 208: 107792, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31707003

RESUMEN

Nitazoxanide (NTZ) is a broad-spectrum drug used in intestinal infections, but still poorly explored in the treatment of parasitic tissular infections. This study aimed to evaluate the in vitro responses of the energetic metabolism of T. crassiceps cysticerci induced by NTZ. The organic acids of the tricarboxylic acid cycle, products derived from fatty acids oxidation and protein catabolism were analyzed. These acids were quantified after 24 h of in vitro exposure to different NTZ concentrations. A positive control group was performed with albendazole sulfoxide (ABZSO). The significant alterations in citrate, fumarate and malate concentrations showed the NTZ influence in the tricarboxylic acid (TCA) cycle. The non-detection of acetate confirmed that the main mode of action of NTZ is effective against T. crassiceps cysticerci. The statistical differences in fumarate, urea and beta-hydroxybutyrate concentrations showed the NTZ effect on protein catabolism and fatty acid oxidation. Therefore, the main energetic pathways such as the TCA cycle, protein catabolism and fatty acids oxidation were altered after in vitro NTZ exposure. In conclusion, NTZ induced a significant metabolic stress in the parasite indicating that it may be used as an alternative therapeutic choice for cysticercosis treatment. The use of metabolic approaches to establish comparisons between anti parasitic drugs mode of actions is proposed.


Asunto(s)
Antiparasitarios/farmacología , Taenia/efectos de los fármacos , Tiazoles/farmacología , Albendazol/análogos & derivados , Albendazol/farmacología , Análisis de Varianza , Animales , Antihelmínticos/farmacología , Citratos/metabolismo , Ciclo del Ácido Cítrico/efectos de los fármacos , Medios de Cultivo/química , Cysticercus/efectos de los fármacos , Cysticercus/metabolismo , Metabolismo Energético/efectos de los fármacos , Fumaratos/metabolismo , Ácidos Cetoglutáricos/metabolismo , Malatos/metabolismo , Neurocisticercosis/tratamiento farmacológico , Ácido Oxaloacético/metabolismo , Ácido Succínico/metabolismo , Taenia/metabolismo
7.
Exp Parasitol ; 208: 107800, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31726054

RESUMEN

The aims of this study were an establishment of the domestic rabbit as an intermediate host for cystic echinococcosis (CE) and to evaluate the potency of the crude germinal layer and the protoscoleces antigens to protect against the CE. Firstly; Two groups of white Newzeland rabbits were infected orally either by 5000 active oncospheres or viable protoscoleces separately. After 20 weeks, the slaughtered rabbits showed the presence of hydatid cysts at different internal organs. Molecular detection of the resulted cysts was conducted. Secondly; 27 rabbits were divided into nine groups (n = 3). Groups 1 and 2 were immunized with the crude germinal layer antigen while the groups 3 and 4 were immunized with the crude protoscoleces antigen. Groups 5 and 6 received the adjuvant mineral oil. Groups 7 and 8 were used as positive control. The last 9 group was kept as a negative control. The obtained results showed a significant high protection percentage of 83.4% and high antibody titer was recorded in groups that received the crude germinal layer antigen comparing with the groups that immunized with the crude protoscoleces antigen as their protection percentage was 66.7% with lower IgG response. In conclusion, the domestic rabbits could be used as a laboratory model for CE. Developing of the germinal layer antigen is more immunogenic than the protoscoleces one and could be used as a promising vaccine. Attention should be directed towards the existing rabbit in the environment adjacent to infected dogs as it could be a part of Echinococcus life cycle.


Asunto(s)
Modelos Animales de Enfermedad , Equinococosis/prevención & control , Echinococcus/inmunología , Conejos , Vacunación , Vacunas , Análisis de Varianza , Animales , Antígenos Helmínticos/inmunología , ADN de Helmintos/aislamiento & purificación , Perros , Echinococcus/genética , Ensayo de Inmunoadsorción Enzimática , Inmunoglobulina G/biosíntesis , Riñón/parasitología , Hígado/parasitología , Pulmón/parasitología , Masculino , Epiplón/parasitología , Potencia de la Vacuna
9.
Exp Parasitol ; 208: 107802, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31730782

RESUMEN

In insects, diet plays an important role in growth and development. Insects can vary their diet composition based on their physiological needs. In this study we tested the influence of diet composition involving varying concentrations of macronutrients and zinc on the immune-tolerance following parasite and pathogen exposure in Spodoptera litura larvae. We also tested the insecticidal potential of Mesorhabditis belari, Enterobacter hormaechei and its secondary metabolites on Spodoptera litura larvae. The results shows macronutrient composition does not directly affect the larval tolerance to nematode infection. However, Zinc supplemented diet improved the immune tolerance. While larvae exposed to bacterial infection performed better on carbohydrate rich diet. Secondary metabolites from bacteria produced an immune response in dose dependent mortality. The study shows that the larvae maintained on different diet composition show varied immune tolerance which is based on the type of infection.


Asunto(s)
Enterobacter/fisiología , Control Biológico de Vectores , Rhabditoidea/fisiología , Spodoptera/inmunología , Análisis de Varianza , Animales , Bioensayo , Carbohidratos/administración & dosificación , Cromatografía Líquida de Alta Presión , Dieta , Enterobacter/inmunología , Enterobacter/patogenicidad , Cromatografía de Gases y Espectrometría de Masas , Tolerancia Inmunológica , Larva/inmunología , Dosificación Letal Mediana , Proteínas/administración & dosificación , Rhabditoidea/inmunología , Rhabditoidea/patogenicidad , Espectroscopía Infrarroja por Transformada de Fourier , Spodoptera/fisiología , Simbiosis , Virulencia , Zinc/administración & dosificación
10.
Food Chem ; 307: 125521, 2020 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-31655264

RESUMEN

The essential oils and antioxidant activity of four Thymus species were evaluated under five light spectra (namely, red, blue, red-blue, white, and greenhouse condition). The highest essential oil yield (4.17%) was observed under red light in T. migricus, while the lowest (1.05%) was observed in T. carmanicus under greenhouse conditions. Light quality also led to difference in essential oil constituents. The highest thymol (66%) was found in T. migricus exposed to blue light, while the least (1.69%) was observed in T. kotschyanus grown under red-blue light. The LED treatments did not induce any significant effect on carvacrol of Thymus species in comparison to the greenhouse condition. Finally, the analysis of variance indicates that the effect of light varied with the studied species. T. migricus performed the highest antioxidant capacity (IC50 = 176.8 µg/mL) under blue light. Overall, essential oil components as well as antioxidants showed significant responses to light emitting diodes wavelengths.


Asunto(s)
Antioxidantes/metabolismo , Luz , Timol/metabolismo , Thymus (Planta)/química , Análisis de Varianza , Antioxidantes/química , Cromatografía de Gases , Análisis por Conglomerados , Efecto Invernadero , Aceites Volátiles/química , Análisis de Componente Principal , Timol/química , Thymus (Planta)/crecimiento & desarrollo , Thymus (Planta)/metabolismo
11.
BJOG ; 127(1): 88-97, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31544327

RESUMEN

OBJECTIVE: To assess the short-term incidence of serious complications of surgery for urinary incontinence or pelvic organ prolapse. DESIGN: Prospective longitudinal cohort study using a surgical registry. SETTING: Thirteen public hospitals in France. POPULATION: A cohort of 1873 women undergoing surgery between February 2017 and August 2018. METHODS: Preliminary analysis of serious complications after a mean follow-up of 7 months (0-18 months), according to type of surgery. Surgeons reported procedures and complications, which were verified by the hospitals' information systems. MAIN OUTCOME MEASURES: Serious complication requiring discontinuation of the procedure or subsequent surgical intervention, life-threatening complication requiring resuscitation, or death. RESULTS: Fifty-two women (2.8%, 95% CI 2.1-3.6%) experienced a serious complication either during surgery, requiring the discontinuation of the procedure, or during the first months of follow-up, necessitating a subsequent reoperation. One woman also required resuscitation; no women died. Of 811 midurethral slings (MUSs), 11 were removed in part or totally (1.4%, 0.7-2.3%), as were two of 391 transvaginal meshes (0.5%, 0.1-1.6%), and four of 611 laparoscopically placed mesh implants (0.7%, 0.2-1.5%). The incidence of serious complications 6 months after the surgical procedure was estimated to be around 3.5% (2.0-5.0%) after MUS alone, 7.0% (2.8-11.3%) after MUS with prolapse surgery, 1.7% (0.0-3.8%) after vaginal native tissue repair, 2.8% (0.9-4.6%) after transvaginal mesh, and 1.0% (0.1-1.9%) after laparoscopy with mesh. CONCLUSIONS: Early serious complications are relatively rare. Monitoring must be continued and expanded to assess the long-term risk associated with mesh use and to identify its risk factors. TWEETABLE ABSTRACT: Short-term serious complications are rare after surgery for urinary incontinence or pelvic organ prolapse, even with mesh.


Asunto(s)
Diafragma Pélvico/cirugía , Prolapso de Órgano Pélvico/cirugía , Mallas Quirúrgicas/efectos adversos , Adulto , Anciano , Anciano de 80 o más Años , Análisis de Varianza , Colposcopía/efectos adversos , Colposcopía/mortalidad , Colposcopía/estadística & datos numéricos , Femenino , Francia/epidemiología , Humanos , Incidencia , Complicaciones Intraoperatorias/etiología , Complicaciones Intraoperatorias/mortalidad , Persona de Mediana Edad , Prolapso de Órgano Pélvico/mortalidad , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/mortalidad , Estudios Prospectivos , Sistema de Registros , Cabestrillo Suburetral/efectos adversos , Cabestrillo Suburetral/estadística & datos numéricos , Mallas Quirúrgicas/estadística & datos numéricos , Centros Quirúrgicos/estadística & datos numéricos , Adulto Joven
12.
Exp Parasitol ; 209: 107829, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31887531

RESUMEN

Ticks, obligatory blood-feeding arthropods, are a major pathogen vector in humans and animals worldwide. Anti-tick vaccines are an exciting alternative to chemical acaricides for controlling these disease-transmitting vectors. However, identification of protective antigens for anti-tick vaccine development is challenging. Different ribosomal proteins play multifunctional roles in tick survival and feeding. Here, we first report the cloning and molecular characterization of ribosomal protein S27 (RPS-27) from the hard tick Haemaphysalis longicornis. We identified a complete open reading frame (ORF) of RPS-27: a 255-bp (base pair) cDNA encoding a mature protein of 84 amino-acid residues with a 9.4-kDa predicted molecular mass. Amino-acid sequence analysis revealed that RPS-27 was highly conserved among different tick and vertebrate animals with identity ranges of 97-98% and 60-85%, respectively. Phylogenetic tree analysis showed that RPS-27 from different tick species clustered together. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the RPS-27 mRNA transcript was expressed in all life stages. At the tissue level, it was more highly expressed in the salivary gland than in the midgut for both the fed and unfed conditions, which indicates a role for RPS-27 in tick feeding. In vitro analysis showed that recombinant RPS-27 (10-RPS-27) was successfully expressed in a pGEMEX-2 vector with an estimated 45-kDa molecular mass. The functional importance of RPS-27 was determined by gene silencing through RNA interference (RNAi). RPS-27 silencing showed a significant (P < 0.05) reduction of feeding abilityand engorgement weight after the blood meal in both nymph and adult female ticks and also significantly (P < 0.05) reduced molting rate in nymph. In addition, RPS-27 silencing in eggs led to abnormalities in shape and hatching. Taken together, our results suggest that RPS-27 is an important molecule that plays multiple roles in the tick life cycle including in both feeding and reproduction. Therefore, RPS-27 is an exciting target for future tick control strategies.


Asunto(s)
Silenciador del Gen , Ixodidae/genética , Sistemas de Lectura Abierta , Proteínas Ribosómicas/genética , Vacunas/genética , Secuencia de Aminoácidos , Análisis de Varianza , Animales , Secuencia de Bases , Western Blotting , Clonación Molecular , ADN Complementario/genética , Electroforesis en Gel de Poliacrilamida , Femenino , Expresión Génica , Ixodidae/clasificación , Ixodidae/inmunología , Ratones , Ratones Endogámicos BALB C , Filogenia , ARN Bicatenario/genética , ARN Bicatenario/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Proteínas Ribosómicas/química , Proteínas Ribosómicas/inmunología , Alineación de Secuencia , Enfermedades por Picaduras de Garrapatas/prevención & control , Enfermedades por Picaduras de Garrapatas/transmisión , Transcripción Genética
13.
Int J Radiat Oncol Biol Phys ; 106(1): 37-42, 2020 01 01.
Artículo en Inglés | MEDLINE | ID: mdl-31229573

RESUMEN

PURPOSE: We sought to characterize temporal trends of radiation oncology resident-reported external beam radiation therapy (EBRT) case experience with respect to various disease sites, including trends in stereotactic radiosurgery and stereotactic body radiation therapy cases. METHODS AND MATERIALS: Summarized, deidentified case logs for graduating radiation oncology residents between 2007 and 2018 were obtained from the Accreditation Council for Graduate Medical Education national summary data report. Mean number of cumulative cases and standard deviations per graduating resident by year were evaluated. Cases were subdivided into 12 disease-site categories using the Accreditation Council for Graduate Medical Education classification. Analysis of variance was used to determine significant differences, and strength of association was evaluated using Pearson correlation. RESULTS: The number of graduating residents per year increased by 66% from 114 in 2007 to 189 in 2018 (P < .001, r = 0.88). The overall mean number of EBRT cases per graduating resident decreased by 13.2% from 521.9 in 2007 to 478.5 in 2018, with a decrease in the ratio of nonmetastatic to metastatic cases per graduating resident. There was significant variation among the disease categories analyzed; however, the largest proportionate decreases were seen in hematologic, lung, and genitourinary malignancies. Stereotactic radiosurgery volume per graduating resident increased from an average of 27.9 cases in 2007 to 50.3 in 2018 (P < .001, r = 0.96). Stereotactic body radiation therapy volume per graduating resident increased as well, from a mean of 6 cases in 2007 to 55.6 cases in 2018 (P < .001, r = 0.99). CONCLUSIONS: We report a longitudinal summary of resident-reported experience in EBRT cases. These findings have implications for future efforts to optimize residency training programs and requirements.


Asunto(s)
Internado y Residencia/tendencias , Neoplasias/radioterapia , Oncología por Radiación/tendencias , Carga de Trabajo , Análisis de Varianza , Competencia Clínica , Neoplasias Hematológicas/radioterapia , Humanos , Internado y Residencia/estadística & datos numéricos , Estudios Longitudinales , Neoplasias Pulmonares/radioterapia , Metástasis de la Neoplasia/radioterapia , Neoplasias/clasificación , Oncología por Radiación/estadística & datos numéricos , Radiocirugia/estadística & datos numéricos , Radiocirugia/tendencias , Radioterapia/estadística & datos numéricos , Radioterapia/tendencias , Estudios Retrospectivos , Factores de Tiempo , Neoplasias Urogenitales/radioterapia , Carga de Trabajo/estadística & datos numéricos
14.
An Bras Dermatol ; 94(6): 704-709, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31789266

RESUMEN

BACKGROUND: Rosacea may result in emotional distress and anxiety. However, data on the presence of generalized anxiety disorder in rosacea patients are scarce. OBJECTIVE: The aim of the study was to detect the frequency and level of anxiety and depression in patients with rosacea. METHODS: A total of 194 consecutive rosacea patients and 194 age- and sex-matched controls were enrolled. Severity of rosacea was assessed in patients according to the criteria of the National Rosacea Society Ethics Committee. Both patients and controls were evaluated by the Generalized Anxiety Disorder 7-item scale, and severity was measured by the Generalized Anxiety Disorder-Adult. RESULTS: Individuals who were diagnosed with an anxiety and/or depressive disorder were more common in patient group (24.7% vs. 7.2%, p<0,01). Female patients were particularly at risk for having generalized anxiety disorder (OR=2.8; 95% CI 1.15-7.37; p=0.02). STUDY LIMITATIONS: Single center study and limited sample size. CONCLUSIONS: Rosacea patients show greater risk of having anxiety disorders, including generalized anxiety disorder. Female patients, those with lower educational levels, those with phymatous subtype, untreated patients, and patients with prior psychiatric morbidity may be at particular risk for anxiety. It is essential to consider the psychological characteristics of patients to improve their well-being.


Asunto(s)
Trastornos de Ansiedad/epidemiología , Trastornos de Ansiedad/etiología , Trastorno Depresivo/epidemiología , Trastorno Depresivo/etiología , Rosácea/complicaciones , Rosácea/epidemiología , Adulto , Análisis de Varianza , Estudios de Casos y Controles , Estudios Transversales , Escolaridad , Femenino , Humanos , Modelos Logísticos , Masculino , Persona de Mediana Edad , Prevalencia , Escalas de Valoración Psiquiátrica , Calidad de Vida , Factores de Riesgo , Rosácea/psicología , Índice de Severidad de la Enfermedad , Estadísticas no Paramétricas , Encuestas y Cuestionarios
15.
Acta Cir Bras ; 34(9): e201900905, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31800679

RESUMEN

PURPOSE: To investigate efficacy of combined use of parecoxib and dexmedetomidine on postoperative pain and early cognitive dysfunction after laparoscopic cholecystectomy for elderly patients. METHODS: The present prospective randomized controlled study included a total of 80 patients who underwent laparoscopic cholecystectomy surgery during January 2016 to November 2017 in our hospital. All patients were randomly divided into 4 groups, the parecoxib group, the dexmedetomidine group, the parecoxib and dexmedetomidine combined group, and the control group. Demographic data and clinical data were collected. Indexes of heart rate (HR), mean arterial pressure (MAP), levels of jugular venous oxygen saturation (SjvO2) and jugular venous oxygen pressure (PjvO2) were recorded at different time points before and during the surgery. The mini-mental state examination (MMSE) score, Ramsay score and Visual Analogue Score (VAS) were measured. RESULTS: Levels of both SjvO2 and PjvO2 were significantly higher in parecoxib group, dexmedetomidine group and the combined group than the control group. Meanwhile, levels of both SjvO2 and PjvO2 in the combined group were the highest. VAS scores were significantly lower in the combined group than all other groups, and total patient controlled intravenous analgesia (PCIA) pressing times within 48 h after surgery were the lowest in the combined group. Both Ramsay and MMSE scores were the highest in the combined group compared with other groups, while were the lowest in the control group. CONCLUSION: The combined use of parecoxib and dexmedetomidine could reduce the postoperative pain and improve the postoperative sedation and cognitive conditions of patients after laparoscopic cholecystectomy.


Asunto(s)
Agonistas de Receptores Adrenérgicos alfa 2/administración & dosificación , Colecistectomía Laparoscópica/efectos adversos , Inhibidores de la Ciclooxigenasa 2/administración & dosificación , Dexmedetomidina/administración & dosificación , Isoxazoles/administración & dosificación , Dolor Postoperatorio/tratamiento farmacológico , /tratamiento farmacológico , Anciano , Anciano de 80 o más Años , Análisis de Varianza , Presión Arterial/efectos de los fármacos , Quimioterapia Combinada , Femenino , Frecuencia Cardíaca/efectos de los fármacos , Humanos , Masculino , Persona de Mediana Edad , Dimensión del Dolor , Estudios Prospectivos , Factores de Tiempo , Resultado del Tratamiento
16.
Braz Oral Res ; 33: e113, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31800864

RESUMEN

The objective of this study was to compare the scores of the Helplessness, Magnification, Rumination, and Catastrophizing factors of the Pain Catastrophizing Scale (PCS) between samples with different pain characteristics. The psychometric properties of the PCS were evaluated in 1,151 Brazilian adults (78.9% female; 38.6 (SD = 10.8) years): 335 had no pain, 390 had been in pain for less than 3 months, 250 had been in recurring pain for more than 3 months, and 176 had been in continuous pain for more than 3 months. Confirmatory factor analysis (CFA) was conducted to verify the fit of the PCS models. Convergent validity and reliability were evaluated. Multi-group analysis was used to estimate the invariance of the factorial model. The global score for the PCS factors was obtained using the regression weight matrix for estimating factor scores from CFA. Analysis of variance was used to compare scores between samples. After excluding three items, the tri-factorial model showed adequate fit. The model parameters were invariant (Δχ2(λ,i,ß,Res); p≥0.05). Individuals experiencing pain showed higher scores for catastrophic thoughts. Individuals with pain for less than 3 months had the highest scores for Rumination (p < 0.001). The PCS showed valid, reliable, and invariant results for the sample of Brazilian adults in no pain or with different pain conditions. The PCS adequately discriminated individuals in pain from those without pain. Among those in pain, Rumination was the only discriminating factor.


Asunto(s)
Catastrofización/psicología , Desamparo Adquirido , Dimensión del Dolor/psicología , Dolor/psicología , Rumiación Cognitiva , Brote de los Síntomas , Adulto , Análisis de Varianza , Brasil , Estudios Transversales , Análisis Factorial , Femenino , Humanos , Masculino , Dimensión del Dolor/métodos , Escalas de Valoración Psiquiátrica , Psicometría , Valores de Referencia , Reproducibilidad de los Resultados , Encuestas y Cuestionarios
17.
Rev Saude Publica ; 53: 106, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31800907

RESUMEN

BACKGROUND: Although the prognosis of differentiated thyroid carcinoma (DTC) therapy is considered excellent over time, some cases have a poorer prognosis and evolve into death. OBJECTIVE: This study aimed to estimate the 5-year specific survival and to identify prognosis factors in a cohort of DTC adult subjects. METHODS: Survival probability was estimated by Kaplan-Meier's method in a retrospective hospital-based cohort study. Comparisons were made by log-rank test. Prognosis factors were identified using Cox risk modeling and crude and adjusted Hazard Ratio measures were obtained. Two models were estimated, considering age grouping of the 7th and 8th editions of TNM. RESULTS: Specific 5-year survival in the cohort was 98.5% (95%CI: 94.2 - 97.5). Considering TNM 7th edition, the risk estimates were 9.88 (95%CI: 1.67 - 58.33) for age group ≥ 55 years, 18.87 (95%CI: 7.38 - 48.29) for individuals with distant metastasis, 6.36 (95%CI: 2.26 - 17.91) for patients who underwent lymphadenectomy and 0.16 (95%CI: 0.06 - 0.43) for those who received radioiodine therapy. For TNM 8th edition, the risk estimates were 10.12 (95%CI: 2.05 - 50.09) for age group ≥ 55 years, 12.43 (95%CI: 4.58 - 33.77) for individuals with distant metastasis, 5.06 (95%CI: 1.82 - 14.05) for patients who underwent lymphadenectomy and 0.19 (95%CI: 0.07 - 0.51) for those who received radioiodine therapy. CONCLUSIONS: This cohort had a very high survival over a 5-year period. The prognosis was negatively influenced by age, distant metastasis and lymphadenectomy, whereas radioiodine therapy was found to be protective.


Asunto(s)
Carcinoma/mortalidad , Derivación y Consulta/estadística & datos numéricos , Neoplasias de la Tiroides/mortalidad , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Análisis de Varianza , Brasil/epidemiología , Carcinoma/patología , Femenino , Estudios de Seguimiento , Humanos , Estimación de Kaplan-Meier , Masculino , Persona de Mediana Edad , Análisis Multivariante , Estadificación de Neoplasias , Pronóstico , Estudios Retrospectivos , Tasa de Supervivencia , Neoplasias de la Tiroides/patología , Factores de Tiempo , Carga Tumoral , Adulto Joven
18.
Int Braz J Urol ; 45(6): 1186-1195, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31808407

RESUMEN

INTRODUCTION: This study aimed to evaluate the effects of transobturator tape (TOT) on overactive bladder (OAB) symptoms and quality of life. MATERIALS AND METHODS: Patients with stress-predominant mixed urinary incontinence (MUI) who had undergone TOT procedures were considered candidates for this research. Preoperative assessment included anamnesis, pelvic examination, cough stress test (CST), and validated symptom severity and quality of life (QoL) questionnaires. The primary outcome, improvement and cure rates of OAB symptoms were determined based on the patient's baseline scores in symptom-related questions in OAB-V8. Secondary outcomes included the success rates of SUI, changes in the QoL score and patient satisfaction rates. RESULTS: A total of 104 patients were included in the study. Sixty-two patients underwent TOT placement alone, and 42 patients underwent TOT placement along with prolapse surgery. The mean follow-up period of the patients was 30.47 months range: 13-52 months. At the fi rst-year follow-up, 52 patients (50.0%) and 59 patients (56.7%) reported cure in preoperative urgency and urgency incontinence, respectively. The objective and subjective cure rates were 96.2% and 56.7%, respectively. A total of 80.7% of the cases had a 15-point improvement in QoL scores. CONCLUSIONS: MUS is not only a gold standard treatment in SUI but also presents as a promising treatment modality in stress-dominant MUI. Although the improvement rates of OAB symptoms signifi cantly decrease over time, QoL and patient satisfaction rates remain higher than any other treatment in this patient group at the third-year follow-up.


Asunto(s)
Calidad de Vida , Cabestrillo Suburetral , Vejiga Urinaria Hiperactiva/cirugía , Incontinencia Urinaria de Esfuerzo/cirugía , Incontinencia Urinaria de Urgencia/cirugía , Adulto , Anciano , Análisis de Varianza , Femenino , Estudios de Seguimiento , Humanos , Persona de Mediana Edad , Medición de Resultados Informados por el Paciente , Satisfacción del Paciente , Periodo Posoperatorio , Periodo Preoperatorio , Estudios Prospectivos , Estadísticas no Paramétricas , Encuestas y Cuestionarios , Factores de Tiempo , Resultado del Tratamiento , Vejiga Urinaria Hiperactiva/fisiopatología , Incontinencia Urinaria de Esfuerzo/fisiopatología , Incontinencia Urinaria de Urgencia/fisiopatología , Adulto Joven
19.
Anticancer Res ; 39(12): 6645-6652, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31810929

RESUMEN

BACKGROUND/AIM: We have previously reported that chemokine (C-X-C motif) receptor 2 (CXCR2) signaling was associated with the malignant progression of gastric cancer (GC). We thus examined the clinicopathological significance of CXCR2 ligands, CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8, in GC. PATIENTS AND METHODS: The expression of CXCR2 ligands in 590 GC cases was investigated by immunohistochemistry. RESULTS: The expression was as follows: CXCL1, 46.2% (257/557); CXCL2, 20.7% (122/590); CXCL3, 17.1% (101/589); CXCL5/CXCL6, 2.9% (17/589); CXCL7, 36.4% (215/590); and CXCL8 1.7% (10/585) of the cases. High invasion depth was correlated with CXCL1 expression. Lymph node metastasis and peritoneal cytology positivity were correlated with high expression of CXCL1 and CXCL7. The prognoses of the CXCL1-positive patients were significantly poorer than those of the CXCL1-negative patients (p<0.001). CONCLUSION: Among the CXCR2 ligands, CXCL7 and especially CXCL1, might play an important role in the malignant progression of GC via CXCR2 signaling.


Asunto(s)
Proteínas de Neoplasias/metabolismo , Receptores de Interleucina-8B/metabolismo , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patología , Anciano , Análisis de Varianza , Quimiocina CXCL1/metabolismo , Quimiocina CXCL2/metabolismo , Quimiocina CXCL5/metabolismo , Quimiocina CXCL6/metabolismo , Quimiocinas CXC/metabolismo , Progresión de la Enfermedad , Femenino , Humanos , Interleucina-8/metabolismo , Ligandos , Metástasis Linfática , Masculino , Invasividad Neoplásica , Pronóstico , Estudios Retrospectivos , Neoplasias Gástricas/mortalidad , beta-Tromboglobulina/metabolismo
20.
Endocrinol. diabetes nutr. (Ed. impr.) ; 66(10): 611-619, dic. 2019. graf, tab
Artículo en Español | IBECS | ID: ibc-184788

RESUMEN

Antecedentes: El ejercicio intervalado de alta intensidad (HIIT) ha sido utilizado ampliamente para combatir los factores de riesgo cardiovascular en población adolescente y adulta, existiendo un vacío de su aplicabilidad en población infantil. Objetivos: Determinar los efectos de un HIIT sobre distintos parámetros antropométricos y cardiovasculares de niños con sobrepeso y obesidad de entre 7 y 9 años de edad. Material y métodos: Cuatro grupos fueron formados: 1) grupo control con sobrepeso (GCS, n = 30, IMC = 21,60 ± 3,72 kg/m2); 2) grupo control con obesidad (GCO, n = 34, IMC = 23,92 ± 3,11 kg/m2); 3) grupo intervención con sobrepeso (GIS, n = 69, IMC = 20,01 ± 1,88 kg/m2), y 4) grupo intervención con obesidad (GIO, n = 141, IMC=24,12 ± 2,66 kg/m2). El IMC, grasa corporal (GC), circunferencia de cintura, razón cintura-estatura, presión arterial sistólica y diastólica, y capacidad cardiorrespiratoria (CRF), fueron evaluadas pre- y postintervención. Resultados: Existieron diferencias significativas en las variables IMC (p < 0,001), GC (p < 0,001) y CRF (p < 0,001) en la comparación entre grupos (control vs. intervención) en pre- y postintervención (GCS vs. GIS y GCO vs. GIO). El GIS (IMC; pre = 20,01 ± 1,88 vs. post = 19,00 ± 2,02, p < 0,001) y el GIO (IMC; pre = 24,12 ± 2,66 vs. post = 23,23 ± 3,23, p < 0,001) disminuyeron su IMC. De igual forma, en el GIS (GC; pre = 21,84 ± 4,97 vs. post = 19,55±4,81%, p < 0,001) y en el GIO (GC; pre = 30,26 ± 11,49 vs. post =26,81 ± 6,80%, p < 0,001) la GC disminuyó. Ambos grupos intervenidos mejoraron su CRF (p < 0,001). Existió una disminución significativa de escolares con obesidad (pre = 66,4% vs. post = 49,6%) (p < 0,001). Conclusión: La intervención realizada en el contexto escolar mejora los parámetros antropométricos y cardiovasculares de los escolares, permitiendo además disminuir la proporción de escolares con obesidad


Background: High-intensity interval training (HIIT) has been widely used to fight cardiovascular risk factors in adolescents and adults, but no data are available on its applicability in children. Objectives: To assess the effects of HIIT on different anthropometric and cardiovascular parameters of overweight and obese children aged 7-9 years. Material and methods: Four groups were formed: 1) an overweight control group (OWCG, n = 30, BMI = 21.60 ± 3.72 kg/m2); 2) an obesity control group (OCG, n = 34, BMI = 23.92 ± 3.11 kg/m2); 3) an overweight intervention group (OWIG, n = 69, BMI = 20.01 ± 1.88 kg/m2), and 4) an obesity intervention group (OIG, n = 141, BMI = 24.12 ± 2.66 kg/m2). BMI, body fat (BF), waist circumference, height-waist ratio, systolic and diastolic blood pressure and cardiorespiratory fitness (CRF) were assessed before and after intervention. Results: There were significant differences in BMI (P < .001), BF (P < .001), and CRF (P < .001) between the groups (control vs. intervention) before and after intervention (OWCG vs. OWIG and OCG vs. OIG). BMI decreased in the OWIG (BMI, 20.01 ± 1.88 at baseline vs. 19.00 ± 2.02 after HIIT, P < .001) and OIG (BMI, 24.12 ± 2.66 at baseline vs. 23.23 ± 3.23 after HIIT, P < .001) groups. Similarly, BF decreased in the OWIG (BF, 21.84 ± 4.97 at baseline vs. 19.55 ± 4.81% after HIIT, P < .001) and OIG (BF, 30.26 ± 11.49 at baseline vs. 26.81 ± 6.80% after HIIT, P < .001) groups. CRF improved in both intervention groups (P < .001). There was a significant decrease in the prevalence rate of schoolchildren with obesity (from 66.4% to 49.6%) (P < .001). Conclusion: The intervention conducted in the school setting improved the anthropometric and cardiovascular parameters of schoolchildren, and also allowed for reducing the proportion of schoolchildren with obesity


Asunto(s)
Humanos , Niño , Obesidad Pediátrica/diagnóstico , Obesidad Pediátrica/terapia , Ejercicio/fisiología , Capacidad Cardiovascular/fisiología , Antropometría , Sobrepeso/complicaciones , Índice de Masa Corporal , Análisis de Varianza
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