Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 8.147
Filtrar
1.
Ecotoxicol Environ Saf ; 208: 111729, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396060

RESUMEN

In this work, carboxyl-functionalized hollow polymer microspheres (CHPMs) was successfully fabricated using poly (styrene-itaconic anhydride) particles as the core template and itaconic anhydride and trans-anethole cross-linked with divinylbenzene as the shell. The desirable microspheres and hollow structure of CHPMs were demonstrated by scanning and transmission electron microscopies, respectively. The characterized CHPMs as an adsorbent was packed into a solid phase extraction column to simultaneously detect the V(V), Cr(III), Cu(II), Cd(II), and Pb(II) in digested food samples by inductively coupled plasma-mass spectrometry (ICP-MS). A series of experimental parameters of solid-phase extraction (SPE) were investigated through vast experiments to improve sensitivity of the proposed method in metal ions detection. The detection limits of the method reached 0.8-3.2 ng L-1 for the target elements, and the relative standard deviations (RSDs) ranging from 1.2% to 3.5% were obtained from eleven parallel experiments using a 1.0 µg L-1 sample solution. The stability allowed the material to withstand more than 15 cycling while the recoveries remained above 88%. In food samples, the detection limits were at 0.20-0.80 µg kg-1, and satisfactory recoveries of 85-104% were obtained in spike tests of laver, fish as well as chicken.


Asunto(s)
Análisis de los Alimentos/métodos , Extracción en Fase Sólida/métodos , Oligoelementos/análisis , Concentración de Iones de Hidrógeno , Microesferas , Polímeros/química , Análisis Espectral
2.
Sensors (Basel) ; 21(2)2021 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-33445458

RESUMEN

The electronic tongue (e-tongue) is an advanced sensor-based device capable of detecting low concentration differences in solutions. It could have unparalleled advantages for meat quality control, but the challenges of standardized meat extraction methods represent a backdrop that has led to its scanty application in the meat industry. This study aimed to determine the optimal dilution level of meat extract for e-tongue evaluations and also to develop three standardized meat extraction methods. For practicality, the developed methods were applied to detect low levels of meat adulteration using beef and pork mixtures and turkey and chicken mixtures as case studies. Dilution factor of 1% w/v of liquid meat extract was determined to be the optimum for discriminating 1% w/w, 3% w/w, 5% w/w, 10% w/w, and 20% w/w chicken in turkey and pork in beef with linear discriminant analysis accuracies (LDA) of 78.13% (recognition) and 64.73% (validation). Even higher LDA accuracies of 89.62% (recognition) and 68.77% (validation) were achieved for discriminating 1% w/w, 3% w/w, 5% w/w, 10% w/w, and 20% w/w of pork in beef. Partial least square models could predict both sets of meat mixtures with good accuracies. Extraction by cooking was the best method for discriminating meat mixtures and can be applied for meat quality evaluations with the e-tongue.


Asunto(s)
Nariz Electrónica/normas , Análisis de los Alimentos/métodos , Contaminación de Alimentos/análisis , Aves de Corral , Carne Roja/análisis , Animales , Fraccionamiento Químico/métodos , Pollos , Culinaria , Análisis de los Alimentos/normas , Alimentos Congelados/análisis , Análisis de los Mínimos Cuadrados , Modelos Estadísticos , Pavos , Agua/química
3.
Food Chem ; 339: 127854, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-32829245

RESUMEN

Gold nanoparticles (AuNPs)-based lateral flow assay (LFA) enables a rapid detection of tetracycline (TET) in food samples but suffers from low sensitivity. Herein, metal-polydopamine framework (MPF), as a label, was employed to load monoclonal antibodies (mAbs) directly as the probe in LFA for highly sensitive sensing of TET. Combining zeolitic imidazolate framework (ZIF-67) and polydopamine (PDA), a stable MPF with large size, well water-dispersible, excellent affinity and optical properties was prepared through a versatile layer-by-layer assembly (LLA) strategy. Under optimized conditions, the biosensor (MPF-LFA) exhibited a great linear relationship in the range of 0.09-6 ng/mL and a detection limit of 0.045 ng/mL for TET detection, which was over 66-fold more sensitive than traditional AuNPs based LFA. Furthermore, the MPF-LFA was successfully applied to the screening of TET in fish, chicken, milk and shrimp samples with satisfied recoveries from 91% to 114%.


Asunto(s)
Antibacterianos/análisis , Análisis de los Alimentos/métodos , Oro/química , Indoles/química , Estructuras Metalorgánicas/química , Polímeros/química , Tetraciclina/análisis , Animales , Técnicas Biosensibles/métodos , Límite de Detección , Nanopartículas del Metal/química , Leche/química , Alimentos Marinos/análisis , Zeolitas/química
4.
Food Chem ; 334: 127471, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32688174

RESUMEN

Optimal QuEChERS (quick, easy, cheap, effective, rugged and safe) conditions with good accuracy, repeatability and precision were established to rapidly extract the European Union (EU) priority polycyclic aromatic hydrocarbons (PAHs) from various food matrices (Category: Poultry and Meat, Fish and seafood, Grains, Soy beans and products, Root vegetables and Coffee). The QuEChERS conditions combined with the established high performance liquid chromatography-fluorescence detection conditions were used to rapidly determine the PAHs in 19 popular cooked foods in Taiwan and their corresponding original materials. These conditions also meet the EU and Taiwan Food and Drug Administration specifications. Charcoal grilled, gas stove grilled and smoked foods had higher PAHs contents, while fried and electric oven-baked/baked foods had lower PAHs contents. In addition to the effects of cooking methods, the contamination of original materials by PAHs in the environment should also have an important impact on the contents of PAHs in these cooked foods.


Asunto(s)
Análisis de los Alimentos/métodos , Contaminación de Alimentos/análisis , Hidrocarburos Policíclicos Aromáticos/análisis , Carbón Orgánico , Fraccionamiento Químico/métodos , Cromatografía Líquida de Alta Presión , Culinaria , Unión Europea , Cromatografía de Gases y Espectrometría de Masas/métodos , Límite de Detección , Carne/análisis , Reproducibilidad de los Resultados , Alimentos Marinos/análisis , Taiwán , Verduras/química
5.
Food Chem ; 334: 127474, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32688175

RESUMEN

Rapid and accurate detection of pesticide residues in food matrices are of great significance to food safety. This study aimed to characterize the fingerprint peaks of 2,4-dichlorophenoxyacetic acid (2,4-D) and to enhance its detection accuracy in food matrices by using terahertz (THz) time-domain spectroscopy. Density functional theory was used to simulate molecular dynamics of 2,4-D peaks (1.35, 1.60, 2.37 and 3.00 THz). Four baseline correction methods, including asymmetric least squares smoothing (AsLS), adaptive iteratively reweighted penalized least squares (AirPLS), background correction (Backcor), baseline estimation and denoising with sparsity (BEADS) were compared and used to eliminate spectral baselines of Zizania latifolia (ZIZLA), rice and maize containing 2,4-D residues, from 0.1 to 4 THz. Based on the peak information of 1.35 THz, the detection limit and accuracy of 2,4-D residues in these food matrices were significantly improved after THz spectral baseline correction, providing a new feasibility for food safety and agricultural applications.


Asunto(s)
Ácido 2,4-Diclorofenoxiacético/análisis , Residuos de Plaguicidas/análisis , Espectroscopía de Terahertz/métodos , Análisis de los Alimentos/métodos , Análisis de los Alimentos/estadística & datos numéricos , Contaminación de Alimentos/análisis , Inocuidad de los Alimentos , Análisis de los Mínimos Cuadrados , Simulación de Dinámica Molecular , Oryza/química , Espectroscopía de Terahertz/estadística & datos numéricos , Zea mays/química
6.
Food Chem ; 334: 127615, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711261

RESUMEN

In this review article, the state of the art of gas sensors based on quartz crystal microbalance (QCM) for fruit freshness detection is overviewed from the aspects of development history, working principle, selection and modification of sensitive materials, and volatile organic compounds detection of fruits. According to the characteristics of respiratory intensity at the stage of fruit ripening, fruits can be divided into respiration climacteric fruits and non-climacteric fruits. In recent years, research has mainly focused on respiration climacteric fruits, such as bananas and mangoes, etc., while related studies on non-climacteric fruits have been rarely reported, except for citrus fruits. The preparation methods and structure design of sensitive materials based on physical/chemical adsorption mechanisms are further discussed according to the odor components that affect the freshness of fruits, namely alkenes, esters, aldehydes and alcohols.


Asunto(s)
Análisis de los Alimentos/métodos , Frutas/química , Tecnicas de Microbalanza del Cristal de Cuarzo/métodos , Compuestos Orgánicos Volátiles/análisis , Adsorción , Alcoholes/análisis , Aldehídos/análisis , Ésteres/análisis , Etilenos/análisis , Análisis de los Alimentos/instrumentación , Calidad de los Alimentos , Gases/análisis , Odorantes/análisis , Tecnicas de Microbalanza del Cristal de Cuarzo/instrumentación
7.
Food Chem ; 334: 127436, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711262

RESUMEN

Milk samples (150 cow and 217 camel milk samples) were analyzed for protein, fat, lactose and total solids by near and mid-infrared transmission spectroscopy. Excellent positive correlations between the two methods were obtained for both types of milk (p < 0.001); for protein (r ≥ 0.96), fat (r ≥ 0.99), lactose (r = 0.82) and total solids (r = 0.90). The mean of the relative difference ((MIR values - NIR values)/0.5 (MIR values + NIR values) × 100%) for cow and camel milk were, for protein (+8.2 & +13.4%), fat (-9.3 & +0.9%), lactose (-5.4 &-0.7%) and total solids (-2.2 &-3.4%), respectively. The difference between the two methods may be due to the effects of differences in milk homogeneity, especially with respect to casein micelles and fat globules.


Asunto(s)
Análisis de los Alimentos/métodos , Lactosa/análisis , Proteínas de la Leche/análisis , Leche/química , Espectrofotometría Infrarroja/métodos , Animales , Calibración , Camelus , Caseínas/análisis , Bovinos , Grasas de la Dieta/análisis , Femenino , Reproducibilidad de los Resultados
8.
Food Chem ; 334: 127558, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711269

RESUMEN

Shellfishes contain plasmalogens correlating to the functions of brain, heart, etc. Herein, a mild acid hydrolysis and hydrophilic interaction chromatography (HILIC) tandem mass spectrometry method was developed for analyzing plasmalogens in six shellfish species. A total of 19 plasmalogen molecular species were successfully identified, including nine phosphatidylcholine plasmalogen (plasPC), seven phosphatidylethanolamine plasmalogen (plasPE), and three phosphatidylserine plasmalogen (plasPS). The quantitative results indicated that mussel (32 µg·mg-1) possessed the highest content of plasmalogens, followed by oyster (21 µg·mg-1) and razor clam (15 µg·mg-1). The statistic models showed that the plasPE P-18:0/20:5 (m/z 748), plasPE P-16:0/22:2 & P-18:0/20:2 (m/z 754) and plasPS were the most contributing difference between shellfishes. The results indicated that this method was sensitive and precise to determine plasmalogens in shellfish, and mussel was demonstrated to be a good choice for the large-scale preparation of plasmalogens.


Asunto(s)
Bivalvos/química , Cromatografía/métodos , Plasmalógenos/análisis , Mariscos/análisis , Espectrometría de Masas en Tándem/métodos , Animales , Análisis de los Alimentos/métodos , Interacciones Hidrofóbicas e Hidrofílicas , Lipidómica/métodos , Ostrea/química , Fosfatidilcolinas/análisis , Fosfatidilserinas/análisis , Plasmalógenos/química
9.
Food Chem ; 334: 127559, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711270

RESUMEN

This study approaches the development of a method for the determination of Ca, Mg, Zn, and Fe in liquid and powdered cow milk. The method is based on sample dissolution assisted by ultrasound energy in tetramethylammonium hydroxide (TMAH) media and determination by flame atomic absorption spectrometry (FAAS). Central composite design (CCD) associated with response surface methodology and desirability function allowed the fast and efficient optimization of the variables involved in the performance of the dissolution. The developed dissolution method allowed Ca, Fe, Zn, and Mg determination in milk samples with adequate analytical characteristics for these determinations. Addition/recovery tests and analysis of a certified reference material of skimmed powdered milk (ERM-BD150) have shown that this method presents enough accuracy to carry out these analyses.


Asunto(s)
Análisis de los Alimentos/métodos , Metales/análisis , Leche/química , Compuestos de Amonio Cuaternario/química , Espectrofotometría Atómica/métodos , Animales , Bovinos , Femenino , Análisis de los Alimentos/estadística & datos numéricos , Análisis Multivariante , Polvos , Solubilidad
10.
Food Chem ; 334: 127607, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711279

RESUMEN

We present a simple and sensitive method for the determination of patulin at µg·kg-1 level in apple-based products. Our method relies on the application of an in-line molecularly imprinted polymer solid-phase extraction microcartridge in capillary electrophoresis coupled with mass spectrometry. Capillary zone electrophoresis method has been developed and parameters affecting the in-line process have been carefully optimized. Validation parameters were assessed for patulin, giving LOQ of 1 µg·kg-1 and linearity range 1-100 µg·kg-1 with R2 ≥ 0.997. The LOQ was below the maximum content of patulin requested by the European Union in this type of products. The precision of the peak area and the migration time were less than 14.9 and 1.6%, respectively. Patulin has been analyzed in the presence of 5-hydroxymethylfurfural, which is the main interference in this kind of matrix. The method was applied to assay patulin content in various apple-based products.


Asunto(s)
Electroforesis Capilar/métodos , Contaminación de Alimentos/análisis , Malus/química , Patulina/análisis , Extracción en Fase Sólida/métodos , Espectrometría de Masas en Tándem/métodos , Análisis de los Alimentos/métodos , Industria de Procesamiento de Alimentos , Furaldehído/análogos & derivados , Furaldehído/química , Límite de Detección , Impresión Molecular , Polímeros/química
11.
Food Chem ; 334: 127608, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711280

RESUMEN

Food analysis to ensure food safety and quality are relevant to all countries. This study aimed to develop a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety (termed RPA-Cas12a-FS). Our data showed that this novel method could be detected via fluorescence intensity for the molecular identification of foodborne pathogenic bacteria, genetically modified crops, and meat adulteration. After optimization, the sensitivity and stability of RPA-Cas12a-FS was further enhanced. The RPA-Cas12a-FS system could specifically detect target gene levels as low as 10 copies in 45 min at 37 °C. The RPA-Cas12a-FS system was sensitive both using standard samples in the lab and using samples from the field, which indicated that this detection method was practical. In conclusion, a simple, rapid, and highly sensitive detection method based on CRISPR-Cas12a was developed for molecular identification in the food safety field without requiring technical expertise or ancillary equipment.


Asunto(s)
Sistemas CRISPR-Cas , Análisis de los Alimentos/métodos , Contaminación de Alimentos/análisis , Microbiología de Alimentos/métodos , Técnicas de Amplificación de Ácido Nucleico/métodos , Proteínas Bacterianas/genética , Proteínas Asociadas a CRISPR/genética , Productos Agrícolas/genética , Endodesoxirribonucleasas/genética , Fluorescencia , Inocuidad de los Alimentos , Carne , Plantas Modificadas Genéticamente/genética , ARN Guia , Recombinasas/genética , Sensibilidad y Especificidad
12.
Food Chem ; 334: 127614, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711282

RESUMEN

Pectin polysaccharide is an important phytochemical with potential biomedical applications. It is commonly measured by time-consuming destructive chemical methods. This work demonstrates the feasibility of using visible and near-infrared hyperspectral imaging (HSI) techniques to rapidly measure pectin polysaccharides in intact mulberry fruits. Based on spatial information provided by HSI images, the representative spectrum of each whole mulberry was accurately extracted without background. The effects of storage temperature on two varieties of mulberries for model establishment were studied. The performances of two spectral ranges obtained by Si and InGaAs CCD detectors for pectin prediction were compared. The best predictions were obtained from dilute alkali soluble pectin and total soluble pectin in Dashi mulberry fruit stored at room temperature, with residual predictive deviation values of 2.317 and 1.935, respectively. Our results show that HSI is a promising alternative to the chemical method to rapidly and nondestructively measure the pectin content.


Asunto(s)
Análisis de los Alimentos/métodos , Frutas/química , Morus/química , Pectinas/análisis , Espectroscopía Infrarroja Corta/métodos , Almacenamiento de Alimentos , Procesamiento de Imagen Asistido por Computador , Modelos Biológicos , Pectinas/química , Temperatura
13.
Food Chem ; 334: 127601, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32712491

RESUMEN

Quantitative labeling of oil compositions has become a trend to ensure the quality and safety of blended oils in the market. However, methods for rapid and reliable quantitation of blended oils are still not available. In this study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to profile triacylglycerols in blended oils, and partial least squares regression (PLS-R) was applied to establish quantitative models based on the acquired MALDI-MS spectra. We demonstrated that this new method allowed simultaneous quantitation of multiple compositions, and provided good quantitative results of binary, ternary and quaternary blended oils, enabling good limits of detection (e.g., detectability of 1.5% olive oil in sunflower seed oil). Compared with the conventional GC-FID method, this new method could allow direct analysis of blended oils, analysis of one blended oil sample within minutes, and accurate quantitation of low-abundance oil compositions and blended oils with similar fatty acid contents.


Asunto(s)
Análisis de los Alimentos/métodos , Aceites Vegetales/análisis , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Cromatografía de Gases/métodos , Ácidos Grasos/análisis , Análisis de los Alimentos/estadística & datos numéricos , Contaminación de Alimentos/análisis , Análisis de los Mínimos Cuadrados , Aceite de Oliva/análisis , Aceites Vegetales/química , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/estadística & datos numéricos , Aceite de Girasol/análisis , Triglicéridos/análisis
14.
Food Chem ; 334: 127472, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32721831

RESUMEN

Pyrrolizidine alkaloids (PAs) are naturally occurring plant toxins associated with severe liver damage if excessive ingestion. Herein, a novel analytical strategy on utilizing direct analysis in real-time mass spectrometry (DART-MS) was developed, and applied in analysis of six representative PAs. The calibration curves in the range of 10-1000 ng·mL-1 were established, and relative standard deviations (RSDs) were less than 10%. The limits of detection (LODs) and limits of quantitation (LOQs) were 0.55-0.85 ng·mL-1 and 1.83-2.82 ng·mL-1, respectively. The feasibility of method was indicated by analysing real samples including Gynura japonica, drug tablets, granules, and fresh cow's milk. Moreover, the results of DART-MS were in good agreement with those observed by high performance liquid chromatography mass spectrometry (HPLC-MS), but consumed less time without chromatographic separation. This research provides a facile fashion for safety assessment of herbal and food products containing PAs and presents promising applications in food, pharmaceutical and clinical analysis.


Asunto(s)
Contaminación de Alimentos/análisis , Espectrometría de Masas/métodos , Preparaciones de Plantas/análisis , Alcaloides de Pirrolicidina/análisis , Animales , Asteraceae/química , Calibración , Bovinos , Cromatografía Líquida de Alta Presión/métodos , Análisis de los Alimentos/métodos , Límite de Detección , Leche/química
15.
Food Chem ; 335: 127631, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32736157

RESUMEN

In this work, sodium 4-styrenesulfonate functionalized polyacrylonitrile nanofibers mat (SS/PAN NFM) was firstly prepared and applied as 96-well plate solid-phase extraction adsorbent for quantitative determination of seven ß-agonists residues in pork samples. The functional modification endowed the SS/PAN NFM with superior adsorption performance for target ß-agonists. The adsorption process is spontaneous (ΔG < 0), the initial adsorption rate can reach 6.03-9.09 mg/g/min and the maximum adsorption capacity is calculated to be 48.3 mg/g at 298 K. Moreover, SS/PAN NFM can be reused for 12 times without degradation in adsorption capability. Combined with UPLC-MS/MS, the limits of detection can reach 0.006-0.24 µg/kg, the recoveries ranged from 87.2% to 111% and the relative standard deviations of intra-day and inter-day precisions were in the scope of 1.75%-11.6% and 5.08%-13.5%, respectively. The obtained results fully demonstrated the practicability of this method in preventing the hazard of ß-agonists residues.


Asunto(s)
Agonistas Adrenérgicos beta/análisis , Agonistas Adrenérgicos beta/aislamiento & purificación , Análisis de los Alimentos/métodos , Nanofibras/química , Poliestirenos/química , Carne Roja/análisis , Extracción en Fase Sólida/métodos , Resinas Acrílicas/química , Agonistas Adrenérgicos beta/química , Adsorción , Animales , Contaminación de Alimentos/análisis , Límite de Detección , Porcinos
16.
Food Chem ; 340: 127930, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-32871357

RESUMEN

It has been remained a challenge to detect trace formaldehyde in complex samples, such as rice flour and duck blood products. In this study, a purge-trap device was designed and used for volatile target detection, which avoided interference adsorptions on enhanced particle surfaces during subsequent surface-enhanced Raman spectroscopy (SERS) analysis. The device produced a low detection limit for formaldehyde of 1 × 10-4 µg/mL in the concentration ranges of 4 × 10-3-4 µg/mL and 1 × 10-4-3 × 10-3 µg/mL. In the process of the detection of duck blood and rice flour, partial least squares regression (PLSR) was adopted for sample analysis. The formaldehyde concentration was calculated and compared to the actual value from the above model with R2 of 0.97, which indicated high accuracy and stability. These results suggested that the proposed method was reliable and suitable for rapid analysis of trace formaldehyde in real products.


Asunto(s)
Análisis de los Alimentos/métodos , Formaldehído/análisis , Espectrometría Raman/métodos , Adsorción , Animales , Patos , Harina , Análisis de los Alimentos/estadística & datos numéricos , Contaminación de Alimentos , Análisis de los Mínimos Cuadrados , Límite de Detección , Nanopartículas del Metal/química , Productos Avícolas/análisis
17.
Food Chem ; 340: 127919, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-32889210

RESUMEN

Non-enzymatic electrochemical sensors for the monitoring of reducing sugars in foods has great potential as a rapid in-situ detection method. This development involved the assembly of a nanoporous platinum structure on a screen-printed carbon electrode (SPCE). The modified electrode was then employed as an amperometric sensing element in a flow injection analysis (FIA) manifold. The system was successfully applied to the rapid detection of reducing sugars in potatoes, without the need for sample preparation. Optimal signals were achieved in phosphate buffer (pH 7.4) at a flow rate of 0.5 mL min-1 and an applied potential of 0.6 V. Experimental results demonstrated the sensor's long-term stability and high selectivity for reducing sugars. This method provides high sample throughput due to a rapid response time of less than five seconds. Reducing sugar values determined were in good agreement with those recorded using a commercially available enzymatic assay kit.


Asunto(s)
Técnicas Electroquímicas/métodos , Electrodos , Análisis de Inyección de Flujo/métodos , Análisis de los Alimentos/métodos , Solanum tuberosum/química , Azúcares/análisis , Carbono/química , Técnicas Electroquímicas/instrumentación , Análisis de Inyección de Flujo/instrumentación , Análisis de los Alimentos/instrumentación , Fructosa/análisis , Jugos de Frutas y Vegetales/análisis , Glucosa/análisis , Nanoestructuras/química , Platino (Metal)/química , Propiedades de Superficie
18.
Food Chem ; 340: 128208, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33022558

RESUMEN

Oligosaccharides analysis is crucial for brewing technology. Herein, we reported a rapid and highly reproducible method for profiling of oligosaccharides in beer using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) by employing a reasonably designed reactive-matrix, 2-phenyl-3-(p-aminophenyl) acrylonitrile (PAPAN). The PAPAN enhanced ionization efficiency of oligosaccharides and improved reproducibility comparing to the use of conventional matrix, 2,5-dihydroxybenzoic acid (DHB). After optimization of sample dilution factor and cationization agents, the distributions of maltooligosaccharides in different brands of beers were unambiguously identified. Since the PAPAN selectively reacts with the reducing end of oligosaccharides, the interferences from matrixes are effectively eliminated. Therefore, the method shows potentials for analysis of oligosaccharides in other foods.


Asunto(s)
Cerveza/análisis , Análisis de los Alimentos/métodos , Oligosacáridos/análisis , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Límite de Detección , Oligosacáridos/química , Reproducibilidad de los Resultados , Factores de Tiempo
19.
Food Chem ; 340: 128162, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33027718

RESUMEN

Studies about the phenolic composition of yellow (Brassica alba), brown (Brassica juncea), and black (Brassica nigra) mustard seeds are still scarce in the literature. Hence, this study describes, for the first time, the use of the QuEChERS extraction method followed by UHPLC-MS/MS analysis for phenolic compound determination in the seeds of these mustard species. Under the optimized extraction and analysis conditions, twenty-one phenolic compounds were evaluated. Six, eleven, and seven were found in B. alba, B. juncea, and B. nigra seeds, respectively. The most abundant phenolic compound was sinapic acid, which was found in amounts ranging from 44 to 82 times higher than the other major compounds found in the mustard seeds, ferulic, 4-hydroxybenzoic and protocatechuic acids. Overall, these results are an important contribution to the characterization of the phenolic composition of the three in natura mustard seeds species, and support future reliable phenolic compounds determination with the QuEChERS method.


Asunto(s)
Costos y Análisis de Costo , Análisis de los Alimentos/métodos , Planta de la Mostaza/química , Fenoles/análisis , Seguridad , Semillas/química , Sinapis/química , Análisis de los Alimentos/economía , Humanos , Pigmentación , Espectrometría de Masas en Tándem , Factores de Tiempo
20.
Food Chem ; 340: 127958, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-32916406

RESUMEN

Although blueberries are widely studied, little information exists on their composition and content of flavonol glycosides. Most studies identify only a few flavonols in blueberries due to separation and identification issues. In the present study, we identified 44 flavonols and chlorogenic acid in 30 samples of Highbush and Rabbiteye blueberry, using HPLC-DAD-ESI-MSn. Highbush group fruits presented mainly quercetin-3-galactoside in their composition, while Rabbiteye group fruits exhibited higher levels of quercetin-3-rhamnoside and quercetin-3-glucuronide. Among the identified flavonols, 8 acylates (acetyl and hydroxymethylglutaroyl) were found, of which quercetin-3-O-[4″-(3-hydroxy-3-methylglutaroyl)]-α-rhamnoside was found for the first time in blueberries. This compound is exclusive to the cultivars Florida and Powderblue, where it is present in high quantities. Glucuronides of syringetin and laricitrin, and rhamnosyl-galactosides of myricetin, quercetin and isorhamnetin were also found for the first time in blueberries. The Principal Component Analysis showed that blueberry groups can be distinguished based on their phenolic compound profile.


Asunto(s)
Arándanos Azules (Planta)/química , Cromatografía Líquida de Alta Presión/métodos , Análisis de los Alimentos/métodos , Fenoles/análisis , Análisis de Componente Principal , Espectrometría de Masa por Ionización de Electrospray/métodos , Frutas/química
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA