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Between the neurotransmission systems modulated by alcohol, the opioid system has been receiving attention in studies that seek to understand its relationship to the effects of addictive substances and different neuropsychiatric disorders. The use of naltrexone stands out in determining the mechanisms of the opioid system, as it acts as an opioid antagonist and consequently generates neurochemical responses. This study aimed to evaluate the pharmacological modulation of opioids on behavioral and neurobiological aspects in adult zebrafish submitted to the protocol of repeated exposure to ethanol and treated with naltrexone. Opioid modulation using naltrexone has been shown to modulate anxiety-like behavior, presenting anxiolytic properties in isolation, in addition to reversing the anxiogenic effect of ethanol through the Novel tank and Light/dark test. Naltrexone increased serotonin and dopamine levels, while ethanol antagonized these effects. In contrast, the interaction between ethanol and naltrexone raised noradrenaline levels. Naltrexone altered glutamate levels, however, ethanol reversed it. Ethanol acted on glutamate transporters increasing their activities, while naltrexone treatment reduced activities. No significant results were found in the pro-oxidant parameters, however, ethanol reduced SOD activity while naltrexone reversed. The same occurred in CAT activity. Also, naltrexone up-regulated the expression of genes related to the dopaminergic, glutamatergic, and opioid systems. The genes used as markers of the inflammatory process and glial activity were modulated by ethanol and together with naltrexone, respectively. Taken together, our findings reinforce the importance of opioid signaling on biochemical and molecular bases related to neuropsychiatric behaviors and diseases, such as anxiety and substance dependence.

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OBJECTIVE: To examine the network structure of depression and anxiety symptoms and their association with Family Health among middle-aged and older people in China. METHOD: This was a quantitative cross-sectional study, a total of 3,365 middle-aged and older people over 45 years were recruited, comprising 1,748 males and 1,617 females. Data were collected by using Patient Health Questionnaire-9, the Generalized Anxiety Disorder-7, and the Short Form of the Family Health Scale. RESULTS: The network structure of anxiety and depression symptoms was stable, and "Fatigue" and "Restlessness" were central symptoms and bridge symptoms. "Family, social or emotional health process" and "Family Healthy Lifestyle" exhibited a significant positive correlation, whereas "Family health resources" and "Suicide" demonstrated a significant negative correlation. CONCLUSION: "Fatigue" and "Restlessness" are the targeted symptoms for preventing comorbid depression and anxiety symptoms among middle-aged and older adults, and the enhancement of "Family health resources" could be crucial for averting the onset of depression and anxiety symptoms within this demographic group.

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BACKGROUND: Undernourishment can significantly affect the serotonergic system, potentially increasing the risk of neuropsychiatric disorders such as depression and anxiety. Probiotic therapy has emerged as a potential modulator of the serotonergic system and brain-derived neurotrophic factor (BDNF). Our hypothesis posits that probiotic treatment positively influences the serotonergic system and BDNF levels in the prefrontal cortex (PFC) and hippocampus (HIP), mitigating the effects of malnutrition. METHODS: We conducted an experiment using 38 adult isogenic rats, divided into four groups: nourished control (n = 9), undernourished control (n = 9), nourished probiotic (n = 10), and undernourished probiotic (n = 10). The animals experienced undernourishment for 10 days, followed by probiotic supplementation while continuing food restriction for an additional 15 days. On the 25th day of the experiment, we euthanized the animals, microdissected their brains, and extracted samples from the HIP and PFC. We performed immunoblotting analysis to assess the expression levels of the following proteins: BDNF, tryptophan hydroxylase 2 enzyme, and 5-HT1A and 5-HT2C serotonergic receptors. RESULTS: Our findings revealed the following effects of probiotic administration: tryptophan hydroxylase 2 expression increased in the PFC of nourished rats (P = 0.033) and in the HIP of undernourished rats (P = 0.013); improved 5-HT2C expression was observed in the PFC under both nutritional conditions (P < 0.01). The proBDNF levels were elevated in the HIP of undernourished rats (P = 0.001). CONCLUSION: Probiotic administration effectively modulated the gut-microbiota-brain axis by enhancing serotonergic system proteins in the prefrontal cortex and hippocampus of both nourished and undernourished rats.

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BACKGROUND: Motivation for the study. The presence of multimorbidity is increasingly frequent in the general population, and this may be associated with mental health problems. Main findings. The presence of multimorbidity, and a great number of chronic conditions, was associated with a higher prevalence of depressive symptoms, anxiety and perceived stress in an adult population in a region of northern Peru. Implications. Our findings suggest the need for adequate mental health management in patients with more than one chronic disease. OBJECTIVE.: To evaluate the association between multimorbidity and mental health in adults aged 30 to 69 years. MATERIALS AND METHODS.: Secondary data analysis of a population-based study conducted in the peri-urban area of Tumbes in the northern coast of Peru. The dependent variables were: depressive symptoms, using the PHQ-9; anxiety symptoms, using the Goldberg scale; and perceived stress, assessed with the Cohen's 14-question scale. The exposure variable was the presence of multimorbidity (presence or not of two or more chronic conditions and the number of chronic conditions). We assessed associations using crude and adjusted Poisson regression models. RESULTS.: Data from 1600 participants were analyzed, mean age was 48.1 years (SD: 10.5), and 50.4% were women. The prevalence of multimorbidity was 15.9%, 23.3% presented depressive symptoms, 42.0% anxiety symptoms and 31.4% had high levels of perceived stress. The multivariable model showed that multimorbidity was associated with a higher prevalence of depressive symptoms (61%, 95%CI: 32% - 98%), anxiety symptoms (46%, 95%CI: 28% - 66%) and high (22%, 95%CI: 14% - 33%) but not moderate levels of perceived stress (6%; 95%CI: 0% - 12%). A higher number of chronic conditions was associated with higher prevalence of depressive symptoms, anxiety symptoms, and perceived stress levels. CONCLUSIONS.: The presence of multimorbidity is associated with a higher prevalence of depressive symptoms, anxiety symptoms, and perceived stress levels. Our results suggest the need for adequate mental health management in patients with multimorbidity.

OBJETIVOS.: Evaluar la asociación entre multimorbilidad y salud mental en personas adultas de 30 a 69 años. MATERIALES Y MÉTODOS.: Análisis de datos secundarios de un estudio poblacional realizado en la zona periurbana de Tumbes en la costa norte del Perú. Las variables dependientes fueron: síntomas depresivos, usando el PHQ-9; síntomas de ansiedad, usando la escala de Goldberg; y estrés percibido, evaluado usando la escala de 14 preguntas de Cohen; mientras que la exposición fue la presencia de multimorbilidad (presencia o no de dos o más condiciones crónicas y el número de condiciones crónicas). Las asociaciones se evaluaron usando modelos regresión de Poisson crudos y ajustados. RESULTADOS.: Se analizaron datos de 1600 participantes, edad media de 48,1 años (DE: 10,5), 50,4% fueron mujeres. La prevalencia de multimorbilidad fue de 15,9%, el 23,3% presentaron síntomas depresivos, 42,0% síntomas de ansiedad y 31,4% tenían niveles altos de estrés percibido. En modelo multivariable, la presencia de multimorbilidad estuvo asociada a una mayor prevalencia de síntomas depresivos (61%, IC95%: 32% - 98%), síntomas de ansiedad (46%, IC95%: 28% - 66%) y niveles altos (22%, IC95%: 14% - 33%), pero no moderados de estrés percibido (6%; IC95%: 0% - 12%). Un mayor número de condiciones crónicas se asoció a mayor prevalencia de síntomas depresivos, síntomas de ansiedad y niveles de estrés percibido. CONCLUSIONES.: La presencia de multimorbilidad se asocia a una mayor prevalencia de síntomas depresivos, de ansiedad y niveles de estrés percibido. Nuestros resultados sugieren la necesidad de un manejo adecuado de la salud mental en pacientes con multimorbilidad.

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Wellbeing levels have been a global concern during the COVID-19 pandemic, but there is a lack of attention to invariance questions that allow a robust examination of wellbeing dynamics across cultures. Questions of temporal stability that are crucial for examining the impact of the pandemic on wellbeing have received even less attention. Some studies suggested that measures may not be stable after the onset of the pandemic. We examine invariance parameters, the factorial structure and variability of wellbeing variables (life satisfaction, pandemic worries, anxiety and depression screenings) across five different cultural contexts from 2020 to 2022 (N = 4387, total observations = 13,161). A three-factor model separating life satisfaction, worry and distress performed best in terms of model fit and parsimony. We observed scalar invariance across times and identified little variability of wellbeing measures during the pandemic, suggesting that wellbeing levels remained stable during the pandemic in each of the countries sampled. In contrast, we only identified metric invariance across countries at each time point, and found a weakening of correlations between life satisfaction and a depressive/anxious symptoms scale in lower income countries. We discuss implications of our findings for discussions of wellbeing dynamics.

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Cross-sectional study investigated the association of fresh or minimally processed foods and ultra-processed food consumption with symptoms of depression, anxiety and stress in students from a Brazilian public university. Undergraduate students admitted in 2022 answered an online questionnaire during their first semester. Consumption of 12 subgroups of fresh or minimally processed foods and 13 of ultra-processed foods on the previous day were investigated (affirmative answer for ≥ 5 subgroups was classified as high consumption). Depression, anxiety and stress were investigated using the DASS-21 and mild to extremely severe symptoms were grouped to be compared with individuals without symptoms. Adjusted logistic regression models estimated the Odds Ratio (OR) of the association between symptoms of depression, anxiety and stress (outcomes) and food consumption (exposures), with a significance level of 5%. A total of 924 students were evaluated, of whom 57.7% presented symptoms of depression, 51.9% of anxiety and 59.4% of stress. A high consumption of fresh or minimally processed foods was observed in 80.3% of the students, with a higher frequency among those without symptoms of depression, anxiety, and stress, while 38.9% showed a high consumption of ultra-processed foods, without differences according to symptoms. High consumption of fresh or minimally processed foods was associated with a lower likelihood of symptoms of depression (OR: 0.62; p=0.011), anxiety (OR: 0.58; p=0.003) and stress (OR: 0.69; p=0.043). No association was found between ultra-processed and mental health outcomes. Actions that support and encourage the consumption of healthy food in the university environment can contribute to mental health outcomes.

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Basal forebrain cholinergic neurons are pivotal for cholinergic signaling in the neocortex and hippocampal formation, crucially implicated in neurodegenerative diseases like late-onset Alzheimer's disease (LOAD), recognition memory impairments, and decision-making. The acetylcholine transporter (VAChT) is essential for loading acetylcholine into synaptic vesicles. Building on our previous findings showing that Ginkgo biloba extract (EGb) preserves recognition memory, we hypothesized EGb would enhance memory in female mice with varying VAChT reductions. We also explored whether reduced cholinergic signaling induces anxiety-like behavior and whether EGb could alleviate such symptoms. Three-month-old female mice with severe VAChT reduction (knockdown homozygotes; VAChT KDHOM), moderate reduction (heterozygotes; VAChT KDHET), and wild-type (WT) mice received the vehicle, 5 mg/kg Donepezil, or EGb at doses of 250, 500, and 1000 mg/kg for 30 days. Memory assessments included aversive tasks like discriminative avoidance memory and non-aversive tasks like object recognition and location memory. We assessed VAChT protein expression in the hippocampal formation (HF) using Western blotting and quantified VAChT-immunopositive cells (IR+) in specific HF subfields (dCA1, dCA3, dDG) using immunohistochemistry. Chronic EGb treatment significantly improved long-term memory in female VAChT KDHOM mice in object recognition and locations memories in a dose-dependent manner, unlike Donepezil. Enhanced memory was correlated with an increase in VAChT-IR+ cells in the dCA1 of VAChT KDHOM mice. Additionally, EGb reduced VAChT-IR+ cells in the dDG of VAChT KDHET mice, which was associated with decreased anxiety-like behavior. These findings suggest that EGb effectively mitigates deficits caused by cholinergic deficiency in hippocampal-dependent memory consolidation, thereby improving our understanding of its role in modulating long-term memory and hippocampal plasticity.

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PURPOSE: To evaluate the effect of real-time self-visualisation (SV) of the procedure during flexible cystoscopy (FC) on pain and anxiety in male and female patients with no prior cystoscopy history. PATIENTS AND METHODS: Between Dec 2022-May 2024, 400 patients who underwent office-based FC were enrolled into prospective randomized study in accordance with CONSORT. Patients were randomised into two groups (SV and no-SV) using sequential (1:1 ratio) randomisation. To ensure equal numbers of male and female patients in each group, one consecutive male patient was assigned to the SV group, while the next male patient was assigned to the non-SV group; the same randomization was done for females. The primary endpoint was to evaluate the pain during FC (during urethral insertion of the cystoscope and bladder examination stages) of both groups. The secondary endpoint was to evaluate anxiety, patient satisfaction, and willingness to undergo the procedure of both groups. RESULTS: In males, significant lower pain scores were detected in SV group during urethral insertion of the cystoscope (1.4 vs. 4.8, p<0.001) and during bladder examination (0.9 vs. 3.1, p<0.001). However, pain scores during urethral insertion of the cystoscope (1.9 vs. 2, p=0.38) and during bladder examination (1.2 vs. 1.3, p=0.63) were statistically similar between two groups in female patients. In both genders, significant lower anxiety levels, higher patient satisfaction and higher willingness to undergo repeat cystoscopy were detected in SV group. CONCLUSION: SV during FC may be beneficial in reducing pain in male patients but not in female patients. SV during FC has a positive effect on anxiety, patients' satisfaction, and willingness to undergo repeat procedures, regardless of gender.

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Alcohol consumption is a worldwide concern that causes 5 % of the global disease burden and contributes to 3 million deaths per year. Several studies suggest an increase in alcohol drinking and alcohol related problems. Alcohol Use Disorder (formerly referred as alcoholism or alcohol addiction) is one of many possible outcomes of an early and prolonged alcohol consumption and it is highly comorbid with anxiety disorders, impulsivity and memory deficits among others. In this review we approach recent data about global and American prevalence of alcohol use and discuss different factors that contribute to alcohol consumption. Furthermore, we revise evidence of ethanol effects on anxiety-like behaviors, impulsivity and spatial memory. Lastly, we look at the Omega-3 fatty acid as a possible course of action in mitigating the aforementioned deleterious effects of alcohol consumption.

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PURPOSE: To test the effects of a physical activity intervention program on symptoms of mental disorders among adolescents. METHODS: This study is a 12-week cluster randomized controlled trial. The sample consisted of 306 adolescents (45,8% male, age: 13,6 [0,72] years; 54.2% female, age: 13.5 [0.69] years), randomized into an intervention group (N = 165) and a control group (N = 141). The physical activity program involved sessions including muscle strengthening and cardiorespiratory exercises and lifestyle guidance. The mental health indicators analyzed were: anxiety, depression, sleep, and psychological well-being. Intervention effects were analyzed by generalized estimating equations. RESULTS: After 12 weeks, no significant improvements were observed in the intervention group for sleep; anxiety; depression, and psychological well-being. In the subgroup analysis, significant group×time interactions were observed for adolescents who were classified with moderate symptoms of depression (mean difference: -0.97, p = 0.002) and anxiety (mean difference: -3.03, p = 0.01) with favorable effects for intervention group. CONCLUSION: After the 12-week intervention period, it can be concluded that 12 weeks of school-based physical activity intervention significantly reduced symptoms of anxiety and depression in adolescents with moderate/severe symptoms. Furthermore, the intervention contributed to the maintenance of psychological well-being, since the control group showed a significant reduction in this mental health indicator.

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Depression and anxiety disorders are prevalent neuropsychiatric conditions worldwide that impose substantial economic and social burdens worldwide. Environmental enrichment (EE) has been employed to investigate how the environment can influence these disorders. While EE is known to mitigate depressive and anxiety phenotypes across various mouse strains, the Balb/C strain exhibits greater sensitivity to different environmental stimuli. In this study, we aimed to assess the long-term effects of EE introduced after weaning on emotional behaviors in adulthood. Balb/C mice were weaned on postnatal day (PND) 21 and exposed to chronic EE for 3, 12, or 24 hours daily until PND 66. Depressive- and anxiety-like behaviors were assessed using the open field, elevated plus maze, and tail suspension tests, along with measurements of corticosterone plasma levels. EE exposure induced emotional dysregulation, evidenced by an increase in anxiety- and depressive-like behaviors. Shorter length of EE (3 h) had less impact on these behaviors compared to longer periods (12 and 24 h). These findings highlight the need for caution when employing the Balb/C strain in EE models, particularly in studies exploring emotional behaviors.

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Early parental care is critical for the development of cortico-limbic circuits regulating stress responses and emotional well-being. Conversely, infant maltreatment can increase susceptibility to mood disorders-such as anxiety and depression-and impair stress-coping abilities. Here, we employed the Scarcity-Adversity Model (SAM) in rats, limiting nesting resources from postnatal days 8-12, to examine its effects on maternal and adult offspring behavior. SAM-exposed mothers exhibited fragmented care and increased violence towards pups. By postpartum day (PPD) 13, maternal fecal corticosterone metabolites (FCM) were elevated, indicating heightened stress. At weaning, SAM dams also showed increased anxiety-like behavior in the Elevated Plus Maze (EPM), suggesting significant emotional alterations. In adulthood, SAM-exposed offspring underwent anxiogenic tests. Both male and female SAM offspring showed increased latency to enter open arms and reduced risk-assessment in the EPM, though females displayed anxiolytic-like behavior in the Light-Dark Box. Male SAM rats had reduced locomotion in the Open Field, earlier onset and increased immobility in the Forced Swim, and increased latency to groom in the Sucrose Splash. When exposed to acute stress, male SAM rats had lower FCM levels, consistent with their passive stress reactivity. These findings confirm SAM induces long-lasting, sex-specific changes in risk-taking, novelty responsiveness, and stress reactions, underscoring the importance of early nurturing in promoting well-being and reducing psychopathological risk.

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Methylmercury (MeHg) is the most harmful organic form of mercury to organisms, especially in the aquatic environment. Therefore, it is crucial to assess the effects of exposure to this contaminant on aquatic biota using multiple biomarkers. In this context, we aimed to analyze the morphophysiological and behavioral effects of sub-lethal exposure to MeHg in tadpoles of Physalaemus ephippifer. To achieve this, larvae of the model species were subjected to a toxicological assay, conducted across five treatments (control; 0.0004 µg/ml; 0.0007 µg/ml; 0.004 µg/ml; and 0.007 µg/ml of MeHg). Following exposure, the tadpoles were subjected to three behavioral assays: escape response, chemical perception, and visual perception. Subsequently, we performed electrocardiographic analysis, cardiac histology, and teratogenic analysis. In the chemical and visual perception assays, tadpoles exposed to MeHg exhibited anti-predator behavior even in the absence of predatory stimuli. Electrocardiographic analyses revealed cardiac hyperexcitability with an increase in heart rate, including tachycardia at the highest concentration, and histological analysis showed physiological cardiac hypertrophy. For teratogenic alterations, sub-lethal concentrations caused an increase in the occurrence of teratogenic effects, such as alterations in the oral apparatus, and body and intestinal morphology. Thus, it can be concluded that the combined data indicate that sub-lethal exposure to MeHg could generate behavioral and physiological changes similar to anxiety in P. ephippifer tadpoles. Therefore, such observed effects are capable of increasing the vulnerability of tadpoles exposed to MeHg, acting as one of the mechanisms leading to the population decline of anurans.

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OBJECTIVES: To compare the impact of COVID-19 on the clinical status and psychological distress of patients with immune-mediated rheumatic disease (IMRD) caused by SARS-CoV-2 infection with that of noninfected IMRD controls during a 6-month follow-up period. METHODS: The ReumaCoV Brazil is a longitudinal study designed to follow IMRD patients for 6 months after COVID-19 (patients) compared with IMRD patients without COVID-19 (controls). Clinical data, disease activity measurements and current treatments regarding IMRD and COVID-19 outcomes were evaluated in all patients. Disease activity was assessed through validated tools at inclusion and at 3 and 6 months post-COVID-19. Fatigue, using FACIT-F (Functional Assessment of Chronic Illness Therapy) and psychological distress, using DASS 21 (Depression, Anxiety and Stress Scale - 21 Items), used to evaluated psychological distress, were evaluated at 6 months after COVID-19 in both groups. The significance level was set as p < 0.05, with a 95% confidence interval. RESULTS: A total of 601 patients were evaluated-321 patients (IMRD COVID-19 + patients) and 280 controls (IMRD COVID-19- patients)-who were predominantly female with similar median ages. Disease activity assessment over a 6-month follow-up showed no significant difference between cases and controls. Although the mean activity scores did not differ significantly, some patients reported worsened disease activity post-COVID-19, particularly in rheumatoid arthritis (RA) (32.2%) and systemic lupus erythematosus (SLE) patients (23.3%). Post-COVID-19 worsening in RA patients correlated with medical global assessment (MGA) and CDAI scores, with a moderate to large effect size. Diabetes mellitus showed a positive association (OR = 7.15), while TNF inhibitors had a protective effect (OR = 0.51). Fatigue, depression, anxiety, and stress were significantly greater in patients than in controls. Worse disease activity post-COVID-19 correlated with worse FACIT-F and DASS-21 scores in RA patients. No significant associations were found between COVID-19 outcomes and post-COVID-19 disease activity, FACIT-F or DASS-21. CONCLUSIONS: Post-COVID-19 IMRD patients exhibited significant fatigue, depression, anxiety, and stress, which can be mistaken for disease activity, despite having similar disease activity scores. The variability in reports on IMRD flares and the potential triggering of SARS-CoV-2 for autoimmune manifestations underscore the need for detailed clinical assessment and a comprehensive approach to managing them.

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BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is the most common Interstitial Lung Disease (ILD). It is characterized by dyspnoea and a progressive decline in lung function, which negatively affects life. This study aimed to evaluate Health-Related Quality of Life (HRQoL) in IPF patients in Latin American countries. METHODS: Six countries (Argentina, Bolivia, Colombia, Chile, Mexico, and the Dominican Republic) enrolled patients with IPF. They answered the Saint George's Respiratory Questionnaire for Idiopathic Pulmonary Fibrosis (SGRQ-I) and the Hospital Anxiety and Depression Scale (HADS). Demographic characteristics, the Torvan index, and a lung function test were also assessed. IPF was diagnosed according to the ATS/ERS/JRS/ALAT 2018 criteria. RESULTS: We enlisted 75 patients diagnosed with IPF; 81% were male, with an average age of 74 ± 7. The total SGRQ-I score was 49 ± 23, with a higher score in the activity domain of 70 ± 23. Torvan index average was 17 ± 6. We found that 28% presented anxiety and 35% depression. Besides, we observed that patients requiring oxygen had a worse quality of life (total SGRQ-I 62 ± 22 vs. 45 ± 22, p = 0.003) without finding differences in antifibrotic therapy. We did not find differences in HRQoL when dividing groups according to their altitude above sea level, except for a higher frequency of anxiety in patients living at sea level. CONCLUSIONS: We found similar data compared to those reported in real-life European populations. We also found that anxiety and depression are prevalent. However, they are often underdiagnosed and, therefore, left untreated.

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Social comparisons are a core feature of human life. Theories posit that social comparisons play a critical role in depression and social anxiety triggering negative evaluations about the self, as well as negative emotions. We investigated the neural basis of social comparisons in participants with major depression and/or social anxiety (MD-SA, n = 56) and healthy controls (n = 47) using functional magnetic resonance imaging. While being scanned participants performed a social comparison task, during which they received feedback about their performance and the performance of a coplayer. Upward social comparisons (being worse than the coplayer) elicited high levels of negative emotions (shame, guilt, and nervousness) across participants, with this effect being enhanced in the MD-SA group. Notably, during upward comparison the MD-SA group showed greater activation than the control group in regions of the default mode network (DMN). Specifically, for upward comparison MD-SA participants demonstrated increased activation in the dorsomedial prefrontal cortex and reduced deactivation in the posteromedial cortex, regions linked to self-referential processing, inferences about other people's thoughts, and rumination. Findings suggest that people with depression and social anxiety react to upward comparisons with a more negative emotional response, which may be linked to introspective processes related to the DMN.

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PURPOSE: The number of delivery workers (DW) has grown significantly with the rise of the platform economy. Evidence suggests that the working conditions faced by these workers are precarious, however, less is known about the health implications among workers. This study aims to investigate the prevalence of mental disorders (MD) and occupational factors related to this condition among DW. METHODS: This is a cross-sectional study, a web survey with DW in Brazil. Data collection lasted four months, starting in February 2022. Anxiety, panic disorder, and depression were measured based on self-reported medical diagnosis. Those with at least one of the three diagnoses were considered to have MD. Cox regression analysis, presenting prevalence ratios, showed the factors related to MD. RESULTS: A total of 563 DW participated in the study. The overall prevalence of MD was 25.6%. Lack of support from the company (PR = 1.71), accelerated work pace (PR = 1.55) and experience of conflicts with company customers (PR-1.48) were psychosocial factors associated to MD. In addition, working in the South, Southeast or Central-West regions is related to a higher prevalence of MD (PR = 2.10). CONCLUSIONS: The high prevalence of MD and its association with psychosocial stressors at work require regulation of the practices of companies operating in the platform economy, aiming to control the intensification of work. In addition to health and social security policies, it is crucial to ensure fair remuneration to reduce the pace of work in a non-strenuous workday, protecting workers from conflicts with dissatisfied customers of the company.

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The T22 protocol is an animal model of forced internal desynchronization, in which rats are exposed to an 11:11 light-dark (LD) cycle. This non-invasive protocol induces the dissociation of circadian rhythms in adult rats, making it possible to study the effects of circadian disruption on physiological and behavioral processes such as learning, memory, and emotional responses. However, the effects of circadian dissociation during other developmental stages, such as adolescence, remain unexplored. Adolescence is a period marked by significant changes in sleep patterns and increased exposure to bright light at night, making it essential to investigate how circadian dissociation may affect this phase of development. This study aimed to evaluate the circadian rhythmicity, cognitive performance and anxiety-like behavior in adolescent Wistar rats under the alignment (aligned T22 group) or misalignment (misaligned T22 group) phases of the T22 cycle. A third group of adolescent rats was maintained in a normal 12:12 LD cycle during the experiment and was used as control group (T24 group). Compared to the control group, adolescent rats under both phases of the T22 cycle exhibited a dissociated circadian rhythm of the locomotor activity and deficits in object recognition memory tasks, without impairments in tasks related to emotional responses. These findings indicate that forced desynchronization impairs recognition memory in adolescent rats, suggesting potential cognitive consequences of internal desynchronization during this critical developmental phase, with relevant implications for public health discussions.

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OBJECTIVES: This study aimed to assess the prevalence and correlates of anxiety-induced sleep disturbance (AISD) in Paraguayan adolescents through sex-stratified analysis. METHODS: This study used the cross-sectional data from Paraguay's 2017 Global School-Based Student Health Survey (GSHS). A total of 3149 in-school adolescents (12-17 years; 51.2 % female) were included in the final analysis. Anxiety-induced sleep disturbance (AISD) was evaluated using the survey question: "During the past 12 months, how often have you been so anxious about something that you could not sleep at night?" Participants could respond on a scale from 1 (never) to 5 (always). For analysis, responses were categorized as follows: "never/rarely/occasionally" indicated no AISD, while "often/always" indicated the presence of AISD. Age-adjusted univariate and multiple logistic analyses were used to determine the correlates of AISD. Regression analysis findings were presented using adjusted odds ratios (AOR) and 95 % confidence intervals (CI), with a p-value <0.05. RESULTS: The overall prevalence of AISD was 9.5 % among the respondents, significantly higher in girls (12 %) than boys (6.7 %). Loneliness, suicidal ideation, not attending physical education classes, and sitting activities were significantly associated with respondents' AISD. In both sexes, those with loneliness and those who had suicidal ideation were more likely to suffer from AISD than their counterparts. Among girls, those who did not attend physical education class and those who engaged in sedentary activities for three hours or more had significantly higher odds of AISD than boys. CONCLUSION: This study found differences in patterns of significant associations between risk factors of AISD for boys and girls. Therefore, different sex-based strategies are recommended to reduce the prevalence of AISD among adolescents in Paraguay.

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INTRODUCTION: Research has shown how mental health among hospital staff worldwide was affected during the COVID-19 pandemic. However, evidence of the physiological status of healthcare and administrative workers at the first level of medical care post-pandemic is scant. Therefore, it is necessary to identify mental health problems among hospital staff following the COVID-19 pandemic. OBJECTIVE: To determine the prevalence of symptoms and severity of depression, anxiety, and stress among health personnel in the primary care post-pandemic of the Institute of Social Security and Services for State Workers in Puebla. MATERIALS AND METHODS: A cross-sectional observational study was conducted on medical and administrative staff, comprising 190 participants who worked during the pandemic. A data collection instrument and the 21 item Depression, Anxiety, and Stress Scale were used to determine the prevalence of symptoms and severity of these three mental health issues. RESULTS: The prevalence of symptoms of depression was 20.5 %, with a moderate predominant severity index (38.5 %), anxiety was 33.2 %, with an extremely severe predominant index (36.5 %), and stress was 30.0 %, with a moderate predominant severity index (36.8 %), respectively. The nursing subgroup, within the medical area staff, exhibited a prevalence of anxiety symptoms of 44.7 %, which was statistically significant (p = 0.008). CONCLUSION: Despite the post-pandemic period during which this study was conducted, the nursing staff was the most affected group in terms of the prevalence of symptoms of anxiety and depression. Another relevant finding was that administrative workers showed an extremely severe intensity of symptoms of depression, anxiety, and stress.