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1.
Huan Jing Ke Xue ; 42(4): 1811-1819, 2021 Apr 08.
Artículo en Chino | MEDLINE | ID: mdl-33742816

RESUMEN

Using solid phase extraction-high performance liquid chromatography tandem mass spectrometry, antibiotics belonging to four classes (i.e. sulfonamides, quinolones, tetracycline, and macrolides) in the surface water of Taihu Lake were monitored monthly for a year. Moreover, the potential ecological risks of antibiotics in Taihu Lake were assessed. During the one-year monitoring, all the eighteen target antibiotics were detected to some extent in the surface water. The detection rates of five sulfonamides (sulfamethoxazole, sulfathiazole, sulfadiazine, sulfadimethazine, and trimethoprim) were higher than 50%. The concentrations of quinolones in the surface water were relatively higher. The average and medium concentrations of ciprofloxacin were 13.0 ng·L-1 and 13.5 ng·L-1, respectively. There were significant differences in the antibiotic pollution during the different months, with the average concentrations of the target antibiotics ranging from 7.3 to 33.5 ng·L-1. The concentration levels were lower from June to October, while higher concentrations were observed from February to May and in November. In the surface water of Taihu Lake, the spatial variations of antibiotics among the 20 sampling sites were not significant, with the average concentrations ranging from 13.0 to 14.3 ng·L-1. During the one-year monitoring, the rates of medium and high risks that the antibiotics posed to algae reached 57.5%. The ecological risks of antibiotics were more severe in April and November, and the quinolones may be the dominant risk factor. This issue should be carefully considered by management authorities.


Asunto(s)
Lagos , Contaminantes Químicos del Agua , Antibacterianos/análisis , China , Monitoreo del Ambiente , Medición de Riesgo , Agua , Contaminantes Químicos del Agua/análisis
2.
Huan Jing Ke Xue ; 42(2): 749-760, 2021 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-33742869

RESUMEN

Emerging contaminants including antibiotics and antibiotic resistance genes (ARGs) have been frequently detected in drinking water resources. In this study, the occurrence of antibiotics and ARGs in various environmental matrices in representative drinking water sources in Jiangsu Province and their influencing factors were explored. Five representative drinking water sources in northern, central, and southern Jiangsu were selected. Water, surface sediment, and epilithic biofilm samples were harvested near the water intakes of each water resource in December 2018 and June 2019. The concentrations and abundances of ten antibiotics, one integrase gene intl1, and seven common ARGs were measured. The results suggest that the concentrations of the target antibiotics and ARGs are relatively low compared to previously reported data in China and elsewhere in the world. The target antibiotics were detected in all of the water sources. The concentrations of sulfonamides in the water, surface sediment, and epilithic biofilm ranged from not found (NF) to 37.4 ng·L-1, NF to 47.3 ng·g-1, and NF to 3759.1 ng·g-1, respectively; the concentrations of quinolones in three matrices were NF-5.3 ng·L-1, 0.4-32.5 ng·g-1, and NF-4220.9 ng·g-1, respectively. The detection rates of the ARGs including sul 1, sul2, tetW, and tetQ were 100%, among which the sulfonamides sul1 and sul2 showed the highest abundance. The absolute abundances of sul1 in the three matrices were 2.48×106 copies·L-1, 3.54×107 copies·g-1, and 1.44×109 copies·g-1, respectively. The abundances of ARGs in the sediments and epilithic biofilms were comparable, and were much higher than in the water body. The phyla Bacteroidetes, Proteobacteris, Firmicutes, Verrucobacteria, and Actinomycetes have proven potential hosts for ARGs and might play an important role in the transmission and diffusion of resistance genes. This study offers baseline information on the presence of antibiotics and ARGs in the drinking water sources of Jiangsu Province, providing a significant theoretical basis for ARGs pollution control and safety guidelines for drinking water resources.


Asunto(s)
Antibacterianos , Agua Potable , Antibacterianos/análisis , China , Agua Potable/análisis , Farmacorresistencia Microbiana/genética , Genes Bacterianos/genética , Recursos Hídricos
3.
Sci Total Environ ; 769: 144642, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33736269

RESUMEN

The occurrence of man-made antibiotics in natural environment has aroused attentions from both scientists and publics. However, few studies tracked antibiotics from their production site to the end of disposal environment. Taking the coastal region of Hangzhou Bay as the study area, the fate of 77 antibiotics from 6 categories in two-step wastewater treatment plants (WTPs, i.e. pharmaceutical WTP and integrated WTP) was focused; and the antibiotics in both dissolved and adsorbed phases were investigated simultaneously in this study. The ubiquitous occurrence of antibiotics was observed in the two-step WTPs, with antibiotic concentrations following the order of PWTP (LOQ - 1.0 × 105 ng·L-1) > IWTPi (for industrial wastewater treatment, LOQ - 3.7 × 103 ng·L-1) > IWTPd (for domestic sewage treatment, LOQ - 1.3 × 103 ng·L-1). And the types of antibiotics detected in excess sludge and suspended particles were in accordance with those in wastewater. Quinolones were invariably dominant in both dissolved and adsorbed fractions. High removal efficiencies (median values >50.0%) were acquired for the dissolved quinolones (except for DFX), tetracyclines, ß-lactams, and lincosamides. Anaerobic/anoxic/oxic achieved the highest aqueous removal of antibiotics among the investigated treatment technologies in the three WTPs. PWTP and IWTP removed 9797 and 487 g·d-1 of antibiotics, respectively; and a final effluent with 126.4 g·d-1 of antibiotics was discharged into the effluent-receiving area (ERA) of Hangzhou Bay. Source apportionment analysis demonstrated that the effluents of IWTPd and IWTPd contributed respectively 39.3% and 8.9% to the total antibiotics in the ERA. The results illustrate quantitatively the antibiotic flows from engineered wastewater systems to natural water environment, on the basis of which the improvements of wastewater treatment technologies and discharge management would be put forward.


Asunto(s)
Aguas Residuales , Contaminantes Químicos del Agua , Antibacterianos/análisis , Bahías , China , Humanos , Aguas del Alcantarillado/análisis , Eliminación de Residuos Líquidos , Aguas Residuales/análisis , Agua , Contaminantes Químicos del Agua/análisis
4.
J Environ Sci (China) ; 103: 12-19, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33743895

RESUMEN

Antibiotic resistance genes (ARGs) as emergence contaminations have spread widely in the water environment. Wild fish may be recipients and communicators of ARGs in the water environment, however, the distribution and transmission of ARGs in the wild fish and relevant water environment were rarely reported. Here, we have profiled ARGs and bacterial communities in wild freshwater fish and relevant water in a peri-urban river using high-throughput qPCR and 16S rRNA gene sequence. A total of 80 and 220 unique ARG subtypes were identified in fish and water samples. Fish and water both showed significant ARG seasonal variations (P < 0.05). The highest absolute abundance of ARGs in fish and water occurred in summer (1.32 × 109 copies per g, on average) and autumn (9.04 × 106 copies per mL), respectively. In addition, the bipartite network analysis showed that 9 ARGs and 1 mobile genetic element continuously shared in fish and water. Furthermore, bacteria shared in fish and water were found to significantly correlate with shard ARGs. The findings demonstrate that bacteria and ARGs in fish and water could interconnect and ARGs might transfer between fish and water using bacteria as a spreading medium.


Asunto(s)
Microbioma Gastrointestinal , Ríos , Animales , Antibacterianos/análisis , Farmacorresistencia Microbiana/genética , Genes Bacterianos , ARN Ribosómico 16S/genética , Agua
5.
Bull Environ Contam Toxicol ; 106(4): 700-706, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33630099

RESUMEN

The presence of multi-resistance to both antibiotics and heavy metals in drinking water poses a significant risk to human health. Herein, we utilized qPCR to assess patterns of antibiotic resistance genes (ARGs), heavy metal resistance genes (HMRGs), and class 1 integron (intI1) gene expression levels in well and tap water samples from four cities in Henan Province, China. The relative abundance of most index values was higher in well water relative to tap water, or was highest in Shangqiu City and lowest in Puyang City on average. The expression of ARG was closely correlated with that of intI1 and HMRG in both well and tap water. Overall, our data highlighted the health threat posed by ARGs in the drinking water supply and underscore the potential for the transfer of these genes between bacteria with the aid of intI1 under selective pressure associated with human activity and heavy metal stress.


Asunto(s)
Integrones , Metales Pesados , Antibacterianos/análisis , China , Ciudades , Farmacorresistencia Microbiana , Genes Bacterianos , Humanos , Metales Pesados/toxicidad , Prevalencia , Agua
6.
Sci Total Environ ; 772: 145516, 2021 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-33571766

RESUMEN

Mining activities are known to generate a large amount of mine tailings and acid mine drainage which contain varieties of heavy metals. Heavy metals play an important role in co-selection for bacterial antibiotic resistance. However, the characteristics of antibiotic resistance genes (ARGs) in mining-affected water environments are still unclear. Here we investigated the pollution of metals, profiles of ARGs, mobile genetic elements (MGEs) and microbial community in mining-affected surface water and groundwater. The results showed that in the tested water samples, the concentrations of Zn and Mn were the highest, and Ni was the lowest. Higher abundances of ARGs with great proportion of sulfonamides, chloramphenicols and tetracyclines resistance genes were found in mining-affected water when compared with those without mining activities. Additionally, there were positive correlations between heavy metals (especially Ni, Zn and Mn) and these ARGs. Linear regression analysis suggested that MGEs were positively correlated with ARGs. In addition, total phosphorus was correlated with ARGs (p < 0.05). The microbial community was different between the mining-affected water and the reference (p < 0.05). Proteobacteria, Bacteroidetes and Actinobacteria were dominant phyla in the surface water and groundwater. Network analysis showed that many ARGs were significantly associated with these dominant bacteria, which suggested they might be potential hosts for these ARGs. These findings provide a clear evidence that the mining activities in the study area had a significant impact on surface water and groundwater to different degrees.


Asunto(s)
Antibacterianos , Agua Subterránea , Antibacterianos/análisis , Farmacorresistencia Microbiana/genética , Genes Bacterianos , Agua
7.
Food Chem ; 351: 129279, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33631615

RESUMEN

The use of the antibiotic enrofloxacin (ENR) in poultry is controversial. A high-performance liquid chromatography coupled to fast-scanning fluorescence detection (HPLC-FSFD) method for the determination of ENR in egg white, egg yolk, and lyophilized samples was developed. In a first analysis, the long-term administration of ENR (100 days) to laying hens was carried out to determine its presence in egg white, yolk, or both. The predominance of ENR was observed in egg white and variations in the weight of egg white and eggshell was evidenced, showing a potential problem in the industry. Eventually, the presence of ENR was confirmed in commercial lyophilized egg white samples in concentration values around 350 µg kg-1. The consumer exposure assessment was estimated for children, adolescents, and adults. The result displayed that, in an intake of lyophilized egg white with food-producing animals, the %ADI exceeds 100%, showing toxicological levels.


Asunto(s)
Antibacterianos/análisis , Huevos/análisis , Enrofloxacina/análisis , Contaminación de Alimentos/análisis , Adolescente , Adulto , Animales , Pollos , Niño , Cromatografía Líquida de Alta Presión , Clara de Huevo/química , Yema de Huevo/química , Humanos , Espectrometría de Masas en Tándem
8.
Food Chem ; 351: 129211, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33636534

RESUMEN

Antibiotic residues in food pose a major threat to the health of humans and animals worldwide. Their trace-level analysis is still too time- and cost-intensive to be adequately covered in routine analysis. Thus, a new high-throughput planar solid-phase extraction method has been developed for rapid screening of 66 antibiotics. Via simple clicks on the image, the autoTLC-MS interface automatically eluted the target analyte zones directly into an orbitrap high-resolution mass spectrometer operated in the variable data-independent acquisition mode. Muscle tissue, cow milk and chicken eggs were analyzed regarding nine different antibiotic classes, including sulfonamides, diaminopyrimidines, lincosamides, pleuromutilins, macrolides, cephalosporins, penicillins, amphenicols and nitroimidazoles. The planar clean-up took 7 min per sample, which is 5-fold faster than the routine state-of-the-art. The screening method has been validated for one representative of each class according to the European Commission Decision 2002/657/EC. Most analytes were successfully detected at half of their required maximum residue limit.


Asunto(s)
Antibacterianos/análisis , Cromatografía en Capa Delgada/métodos , Residuos de Medicamentos/análisis , Análisis de los Alimentos/métodos , Contaminación de Alimentos/análisis , Espectrometría de Masas/métodos , Extracción en Fase Sólida/métodos , Animales , Antibacterianos/aislamiento & purificación , Residuos de Medicamentos/aislamiento & purificación
9.
Ecotoxicol Environ Saf ; 213: 111981, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33592372

RESUMEN

Environmental pollution due to resistance genes from livestock manure has become a serious issue that needs to be resolved. However, little studies focused on the removal of resistance genes in simultaneous processing of livestock feces and urine. This study investigated the fate of antibiotic resistance genes (ARGs), metal resistance genes (MRGs), and class 1 integron-integrase gene (intI1) during thermophilic fermentation of swine manure in an ectopic fermentation system (EFS), which has been regarded as a novel system for efficiently treating both feces and urine. The abundances of MRGs and tetracycline resistance genes were 34.44-97.71% lower in the EFS. The supplementation of heavy metals significantly increased the abundance of intI1, with the enhancement effect of copper being more prominent than that of zinc. The highest abundances of resistance genes and intI1 were observed at high Cu levels (A2), indicating that Cu can increase the spreading of resistance genes through integrons. Network analysis revealed the co-occurrence of ARGs, MRGs, and intI1, and these genes potentially shared the same host bacteria. Redundancy analysis showed that the bacterial community explained most of the variations in ARGs, and environmental factors had influences on ARGs abundances by modulating the bacterial community composition. The decreased Sphingomonas, Comamonas, Acinetobacter, Lactobacillus, Bartonella, Rhizobium, and Bacteroides were mainly responsible for the reduced resistance genes. These results demonstrate that EFS can reduce resistance genes in simultaneous processing of livestock feces and urine.


Asunto(s)
Farmacorresistencia Microbiana/genética , Fermentación/fisiología , Genes Bacterianos , Estiércol/microbiología , Metales Pesados/análisis , Animales , Antibacterianos/análisis , Bacterias/efectos de los fármacos , Cobre , Integrones , Ganado , Estiércol/análisis , Porcinos , Tetraciclina/farmacología
10.
Ecotoxicol Environ Saf ; 208: 111724, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396055

RESUMEN

In recent years, in order to promote animal growth and reduce the risk of disease, a variety of antibiotics are frequently added to the animal feed of livestock and poultry. However, these antibiotics can not be fully digested by animals and most of them are excreted with feces, consequently causing the enrichment of antibiotic resistance genes (ARGs) and huge environmental risks. Nowadays, composting is a better option to solve these problems. Accordingly, this study explored the effects of co-composting swine manure with different inoculants dominated by Phanerochaete chrysosporium (p), Aspergillus niger (a), and Bacillus licheniformis (b) on the simultaneous removal of multiple antibiotics and resistance genes and evolution of the bacterial community. The results showed that the highest removal extent of tetracycline and oxytetracycline occurred in pile D (p:b:a=1:5:5, biomass) reaching 89.2% and 87.8%, respectively, while the highest removal extent of doxycycline and enrofloxacin occurred in pile A (p:b:a=1:0:0, biomass) reaching 98.6% and 89%, respectively. Compared with the levels in pile B (control check), in pile D, ARGs, except those for sulfonamides, decreased by 1.059 × 10-3-6.68 × 10-2 gene copies/16S rRNA copies. Inoculation with p alone effectively reduced intI1 and intI2. Canonical correspondence analysis (CCA) that microbial community structure evolution had a greater influence on ARGs than environmental factors. In summary, this study provided a feasible way to efficiently remove the antibiotics and antibiotic resistance genes in pig manure.


Asunto(s)
Antibacterianos/análisis , Farmacorresistencia Microbiana/genética , Estiércol/microbiología , Alimentación Animal/análisis , Animales , Antibacterianos/metabolismo , Bacterias/efectos de los fármacos , Compostaje/métodos , Genes Bacterianos , Ganado , Microbiota/efectos de los fármacos , Oxitetraciclina/farmacología , ARN Ribosómico 16S , Porcinos , Tetraciclina/farmacología
11.
Environ Monit Assess ; 193(2): 77, 2021 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-33474619

RESUMEN

The presence of antimicrobials in wastewater (WW), surface water (SW), groundwater (GW), and even in potable water from treatment plants has been reported from many countries. Their presence in the water sources is causing the rise and spread of antimicrobial resistance (AMR), thereby threatening our public health, global economy, and development. This necessitates the constant monitoring of these compounds along with the evaluation of their associated risk to aquatic organisms. In this study, GW, WW, and SW samples from different parts of West Bengal (India) were analyzed using the SPE-HPLC-DAD method for detecting two frequently used fluoroquinolones (ciprofloxacin, CIP and ofloxacin, OFL). The highest concentration of CIP and OFL was 5.75 µg/L (GW) and 17.84 µg/L (WW), respectively. The antimicrobial activity was determined against Escherichia coli and Staphylococcus aureus isolates from WW against CIP, which showed that Escherichia coli and Staphylococcus aureus had developed ~ 69 and ~ 12 times resistance compared to their respective pure strains. The risk assessment showed that CIP poses an insignificant threat to fish and Daphnia (RQ < 1) but a significant threat to green algae and Microcystis aeruginosa (RQ> > 1). OFL concentration also poses a great threat to all the organisms for which the assessment was made (RQ> > 1). Moreover, risk assessment in terms of AMR showed that the present level of these antimicrobials in different water sources could cause the development of resistance among the microbial community (RQ > 1). These results emphasize the need for constant monitoring of pharmaceutical compounds, especially antimicrobials, to be kept under check.


Asunto(s)
Antiinfecciosos , Monitoreo del Ambiente , Animales , Antibacterianos/análisis , Antibacterianos/toxicidad , Antiinfecciosos/toxicidad , Ciprofloxacino , India , Pruebas de Sensibilidad Microbiana
12.
Food Chem ; 347: 129034, 2021 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-33486363

RESUMEN

Herein, an optical sensor based on nanostructured molecularly imprinted polymer (MIP) coated on a luminescent zirconium metal-organic framework (MIP/Zr-LMOF) is introduced, and its performance is investigated for the fluorescent determination of chloramphenicol (CAP) antibiotic residues in milk and honey. To fabricate the sensor, the surface of Zr-LMOF is modified with MIP in the presence of CAP template, resulting in the introduction of recognition sites for antibiotic molecules. The porous structure of Zr-LMOF with specific binding sites for CAP recognition benefiting from coated MIP leads to selective and sensitive detection of antibiotic. The probe yields a linear range for detection of CAP in trace concentrations (0.16-161.56 µg.L-1) and provides a detection limit of 0.013 µg.L-1. Acceptable recoveries are achieved for antibiotic in real samples, which are consistent with that obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS), confirm the favorable performance of sensor for accurate determination of CAP in practical applications.


Asunto(s)
Cloranfenicol/análisis , Miel/análisis , Estructuras Metalorgánicas/química , Leche/química , Nanoestructuras/química , Circonio , Animales , Antibacterianos/análisis , Cromatografía Liquida , Luminiscencia , Espectrometría de Masas en Tándem
13.
Ecotoxicol Environ Saf ; 211: 111955, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33497859

RESUMEN

Anthropogenic activities especially water pollution can affect the diversity and composition of microbial communities and promote the spread of antibiotic resistance genes (ARGs). In this study, water samples and guppies (Poecilia reticulata) were sampled from six sampling sites along the Uberabinha River in southeastern Brazil, both microbial communities and ARGs of surface waters and intestinal microbiota of guppies (Poecilia reticulata) were detected. According to the results of 16S rRNA amplicon sequencing, Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were dominant phyla in both water and intestinal microbiota, but the abundance of putative pathogens was higher at heavily polluted sites. Up to 83% of bacteria in intestinal microbiota originated from water microbiota; this proportion was relatively higher in less polluted compared to polluted environments. ARGs providing resistance of tetracyclines and quinolones were dominant in both water and gut microbiota. The relative abundances of class I integrons and ARGs were as high as 1.74 × 10-1/16S rRNA copies and 3.61 × 10-1/16S rRNA copies, respectively, at heavily polluted sites. Correlation analysis suggests that integrons and bacteria play key roles in explaining the widespread occurrence of ARGs in the surface, but not in intestinal microbiota. We could rule out the class I integrons a potential intermediary bridge for ARGs between both types of microbiomes. Our results highlight the tight link in microbial communities and ARGs between ambient microbiota of stream ecosystems and intestinal microbiota of fish. Our study could have far-reaching consequences for fisheries and consumer safety and calls for investigations of gut microbiota of target species of both commercial fisheries and recreational (hobby) angling.


Asunto(s)
Farmacorresistencia Microbiana/genética , Microbioma Gastrointestinal/efectos de los fármacos , Genes Bacterianos , Poecilia/fisiología , Contaminación del Agua/estadística & datos numéricos , Animales , Antibacterianos/análisis , Bacterias/genética , Brasil , Integrones , Microbiota/genética , Poecilia/genética , Poecilia/microbiología , Quinolonas/análisis , ARN Ribosómico 16S/genética , Ríos/microbiología , Agua/análisis , Contaminación del Agua/análisis
14.
J Mater Chem B ; 9(4): 1123-1130, 2021 01 28.
Artículo en Inglés | MEDLINE | ID: mdl-33427845

RESUMEN

Antibiotic contaminants in aqueous media pose a serious threat to human and ecological environments. Therefore, it is necessary to develop robust strategies to detect antibiotic residues. For this purpose, a self-assembly and in situ electrochemical reduction method is utilized to tailor silver nanoparticles (AgNPs)-coated GNRs (AgNPs/GNRs) large-scale vertical arrays. These AgNPs/GNRs arrays exhibit outstanding surface-enhanced Raman scattering (SERS) activities because of abundant Raman hot-spots among the adjacent AgNPs and GNRs, but also excellent stability and reproducibility due to the close-packed arrayed nanostructure. These remarkable features validate this arrayed substrate for high-sensitivity 4-aminothiophenol analysis with a detection limit of 0.35 pM and self-cleaning via electrochemical stripping of the adsorbed analytes and AgNPs from the GNRs arrays, therefore realizing renewable SERS applications. Moreover, the distinct SERS performance of AgNPs/GNRs arrays is verified via the analysis of multiplexed antibiotics at tens of picomolar level and no apparent changes of SERS activities are observed when recyclability is explored. The result demonstrates that the proposed AgNPs/GNRs arrays provide a novel strategy for avoiding conventional, disposable SERS substrates, as well as expanding SERS applications for simultaneous sensing and stripping of environmental contaminants.


Asunto(s)
Compuestos de Anilina/análisis , Antibacterianos/análisis , Oro/química , Nanotubos/química , Plata/química , Compuestos de Sulfhidrilo/análisis , Estructura Molecular , Tamaño de la Partícula , Espectrometría Raman , Propiedades de Superficie
15.
Environ Monit Assess ; 193(2): 98, 2021 Jan 28.
Artículo en Inglés | MEDLINE | ID: mdl-33511434

RESUMEN

To assess the impact of antibiotic pollution to the ecosystem in urban water, the occurrence, seasonal, and spatial distributions, potential sources, and ecological risks of 18 targeted antibiotics in urban river, Pearl River located in Guangzhou city, were investigated. Surface water samples were sampled from 24 sites in Guangzhou center of Pearl River during dry and wet seasons. The results indicated that the concentrations of antibiotic residues were at the nanogram per liter level, except sulfamethazine (SMD) (µg/L). Sulfonamides (SAs) were the dominant antibiotics, contributing 60.4-65.0% to the total antibiotics. The concentrations of SAs, fluoroquinolones (QUs), macrolides (MLs), tetracyclines (TCs), and lincosamides (LCs) were higher in dry season than those in wet season at most sampling sites, which possibly resulted from the dilution effect of heavy rainfall. The concentrations of the antibiotic residues in Guangzhou were comparable or higher than other urban rivers. The calculation on risk quotients indicated that erythromycin-H2O (ETM-H2O) and tetracycline (TC) were of high risks. The source identification by the Pearson correlation analysis and principal component analysis-multiple linear regression (PCA-MLR) method suggested that municipal wastewater treatment plants were primary sources of antibiotics. These results would provide important information for the environmental protect.


Asunto(s)
Ríos , Contaminantes Químicos del Agua , Antibacterianos/análisis , China , Ciudades , Ecosistema , Monitoreo del Ambiente , Medición de Riesgo , Agua , Contaminantes Químicos del Agua/análisis
16.
Food Chem ; 346: 128920, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-33387836

RESUMEN

A high-throughput method using a new ZIF-8@GO thin-film microextraction coating was established for determining macrolides and lincosamides in honey. The coating preparation parameters (ZIF-8@GO synthesis conditions, coating material proportions, dipping time) and analysis parameters (sample diluent solvent, adsorption and desorption conditions using the ZIF-8@GO coating) were optimized. The optimized parameters were: diluent solvent sodium carbonate/sodium bicarbonate buffer solution (pH 9), adsorption time 45 min, desorption time 5 min, desorption solvent 45:40:15 v/v/v methanol/acetonitrile/water. The extracted targets were determined by ultra-high performance liquid chromatography tandem mass spectrometry. The recoveries of 10 analytes were 67.5-107.2% and the detection and quantification limits were 0.1-0.4 and 0.4-1.4 µg/kg, respectively. The method could analyze 96 samples per run. The minimal manual time and effort is required since the bulk of the sample processing is fully automated. It was a useful and efficient method for monitoring drug residues and was successfully used to analyze real samples.


Asunto(s)
Antibacterianos/análisis , Miel/análisis , Lincosamidas/análisis , Microextracción en Fase Líquida/métodos , Macrólidos/análisis , Acetonitrilos , Cromatografía Líquida de Alta Presión/métodos , Límite de Detección , Espectrometría de Masas en Tándem/métodos
17.
ACS Appl Mater Interfaces ; 13(4): 5357-5367, 2021 Feb 03.
Artículo en Inglés | MEDLINE | ID: mdl-33471500

RESUMEN

Herein, the hydrophobic carboxyl-functionalized ionic liquid (IL-COOH) was encapsulated into the prepared Fe3O4@Zr-MOFs, and the novel water-stable IL-COOH/Fe3O4@Zr-MOF nanocomposites were first synthesized. The polydopamine-functionalized Fe3O4 was introduced to construct the core-shell structure via layer-by-layer modification, and the controlled growth of Zr-MOFs was achieved, which realized the adjustment of charged properties of nanocomposites and simplified the adsorption or extraction process. The IL-COOH/Fe3O4@Zr-MOFs were fully studied by IR, HNMR, XRD, N2 adsorption-desorption isotherms, TEM, EDS mapping, VSM, and so on. Then, they were employed for the selective adsorption and detection of fluoroquinolone antibiotics (FQs). The adsorption isotherms and kinetics demonstrated that the adsorption process followed a pseudo-second-order kinetic model and the Langmuir isotherm model. Among them, IL-COOH/Fe3O4@UiO-67-bpydc showed the best adsorption performance, and the maximum adsorption capacity of ofloxacin was 438.5 mg g-1. Coupled magnetic solid-phase extraction with HPLC-DAD, a convenient, sensitive, and efficient method for extraction and detection of FQs in environmental water, was developed based on IL-COOH/Fe3O4@UiO-67-bpydc. The recoveries of environmental water were ranging from 90.0 to 110.0%, and the detection limits were lower than 0.02 µg L-1. The novel functionalized composites served as solid-phase adsorbents and liquid-phase extractants. This study also provided a promising strategy for designing and preparing multi-functionalized nanocomposites for the removal or detection of pollutants in environmental samples.


Asunto(s)
Antibacterianos/análisis , Fluoroquinolonas/análisis , Líquidos Iónicos/química , Estructuras Metalorgánicas/química , Nanocompuestos/química , Contaminantes Químicos del Agua/análisis , Adsorción , Antibacterianos/aislamiento & purificación , Cromatografía Líquida de Alta Presión/métodos , Fluoroquinolonas/aislamiento & purificación , Límite de Detección , Nanopartículas de Magnetita/química , Extracción en Fase Sólida/métodos , Agua/análisis , Contaminantes Químicos del Agua/aislamiento & purificación
18.
Sci Total Environ ; 768: 144526, 2021 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-33450684

RESUMEN

Many pathogenic bacteria are adapted to live in aquatic habitats, which makes rivers possible sources and spread pathways of antibiotic resistance, since they usually receive effluents from wastewater treatment plants (WWTP), possibly containing antibiotic residues and also antibiotic-resistant bacteria. This study investigates different monitoring strategies to identify the occurrence of antibiotic-resistant bacteria in rivers. We analyzed the presence of 13 antibiotic resistance genes (ARGs) and seven gene markers for facultative pathogenic bacteria (FPB) with qPCR in sampling sites upstream and downstream of a small WWTP in Southern Germany. Five sampling campaigns were conducted from February to June 2019. Surface water, sediment, and biofilm samples were analyzed. The biofilm was collected from an artificial sampler placed in the river. blaTEM, ermB, tetM, and sul1 genes were detected in all samples analyzed. The results showed there was a previous background in the river, but the WWTP and the water quality of the river influenced the concentration and occurrence of ARGs and FPB. Genes representing resistance against strong or last-resort antibiotics, such as mecA, blaCMY-2, blaKPC-3, and mcr-1, and multidrug resistance were also detected, mainly in samples collected downstream of the WWTP. Downstream of the WWTP, the occurrence of ARG and FPB correlated with ammoniacal nitrogen, while upstream of the WWTP correlated with turbidity, suspended solids, and seasonal factors such as UVA radiation and the presence of macrophytes. Biofilm samples presented higher abundances of ARGs and FPB. The biofilm sampler was efficient and allowed to collect biofilms from specific periods, which helped to identify seasonal patterns.


Asunto(s)
Antibacterianos , Purificación del Agua , Antibacterianos/análisis , Biopelículas , Farmacorresistencia Microbiana/genética , Genes Bacterianos , Alemania , Aguas Residuales , Agua , Calidad del Agua
19.
Environ Sci Pollut Res Int ; 28(13): 16380-16393, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33387316

RESUMEN

This study investigates the presence of the different classes of micro-pollutants such as pharmaceutical active compounds (PhACs) (20 antibiotics, 8 analgesics and anti-inflammatories, 5 cytostatic agents, 7 ß-blockers, 4 lipid regulators, 13 psychiatrics, 1 antidiabetic, 1 receptor antagonist, 1 local anaesthetic, 1 antihypertensive and their 5 metabolites), hormones (8 compounds), X-ray contrast agents (6 compounds), benzotriazoles (3 compounds) and pesticides (6 compounds), and antibiotic resistance in hospital wastewater (HWW) of a medical faculty in Istanbul, Turkey. In addition, the seasonal variations of the selected PhACs and X-ray contrast agents and antibiotic resistance were evaluated for 2 years in a total of eight samples. In the PhACs, sulfamethoxazole and its metabolite (4 N-acethyl-sulfamethoxazole) in the antibiotic group and paracetamol in the analgesic and anti-inflammatory group were found at 100% of frequency and the highest concentrations as 35, 43 and 210 µg/L, respectively. The mean concentrations of psychiatric compounds were found less than 0.25 µg/L except carbamazepine (1.36 µg/L). Bisphenol A in hormone group had the highest concentration up to 14 µg/L. In the hormone group compounds, 17-α-Ethinylestradiol and 17-ß-Estradiol were detected at lower mean concentrations of 0.2 and 0.05 µg/L, respectively. 1H-benzotriazole had the highest concentration with the mean concentration of 24.8 µg/L in benzotriazole group compounds. The compounds in X-ray contrast agents group were noted as compounds detected at the highest concentration in HWW up to 3000 µg/L. Antibiotic resistance against azithromycin, clindamycin and trimethoprim-sulfamethoxazole antibiotics was observed around 50% in the winter period. The seasonal variation was detected for the most of the investigated PhACs, especially in antibiotic group which was in line with those significant differences in antibiotic resistance rates in the studied antibiotics between winter and summer seasons.


Asunto(s)
Preparaciones Farmacéuticas , Contaminantes Químicos del Agua , Antibacterianos/análisis , Farmacorresistencia Microbiana , Monitoreo del Ambiente , Hospitales , Estaciones del Año , Turquia , Aguas Residuales/análisis , Contaminantes Químicos del Agua/análisis
20.
Food Chem ; 348: 129097, 2021 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-33515941

RESUMEN

The aim of this study was to isolate and identify antibacterial peptides (ABPs) produced by lactic acid bacteria (LAB) in Chinese pickles. The cell-free supernatant collected from the culture of LAB with antibacterial activity against Staphylococcus aureus was used to purify ABPs. A total of 14 strains of LAB were found to have antibacterial activity. Among them, Lactobacillus fermentum (L. fermentum) SHY10 exhibited the most effective antibacterial activity. The antibacterial activity of cell-free supernatant reached the highest level after 20 h of L. fermentum SHY10 culture. Three novel ABPs were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In particular, the NQGPLGNAHR peptide showed antibacterial activity with an IC50 value of 0.957 mg/mL. In addition, molecular docking analysis revealed that this peptide interacted with DNA gyrase and dihydrofolate reductase by salt bridge formation, hydrogen bond interactions, and metal contact.


Asunto(s)
Antibacterianos/análisis , Antibacterianos/aislamiento & purificación , Lactobacillus fermentum/metabolismo , Péptidos/análisis , Péptidos/aislamiento & purificación , Antibacterianos/biosíntesis , Alimentos y Bebidas Fermentados/microbiología , Péptidos/metabolismo
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