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1.
Addict Behav ; 136: 107464, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36067636

RESUMEN

BACKGROUND: As daily engagement with social networking sites (SNS) increases globally, identifying and understanding the risk factors associated with problematic SNS use is of utmost importance. Researchers are interested in understanding internalizing symptoms as both a risk factor and a negative consequence of problematic SNS use. Prior research has proposed rumination alongside internalizing symptoms as a risk factor, though limited research has examined these associations across different cultures. OBJECTIVE: The present study examined the indirect associations between internalizing symptoms (specifically depressive and social anxiety symptoms) and problematic SNS use via rumination among a cross-cultural sample. METHOD: Participants were 8,912 (70.8 % female; Mean age = 20.25, SD = 3.95) college students recruited across seven countries (U.S., Canada, Spain, England, Argentina, Uruguay, and South Africa) who completed measures of internalizing symptoms, rumination, weekly SNS use, and problematic SNS use. RESULTS: We found that higher internalizing symptoms were associated with more problematic SNS use via higher ruminative thinking. Specifically, problem-focused thoughts (a rumination subtype) uniquely accounted for 22.89% and 28.15% of the total effect of depressive and social anxiety symptoms on problematic SNS use, respectively. Other rumination subtypes (i.e., anticipatory thoughts and repetitive thinking) also demonstrated significant indirect effects, though weaker effects than for problem-focused thoughts. Findings were invariant across countries. CONCLUSIONS: These findings provide support for further exploring the role rumination plays in determining and comparing problematic SNS use cross-culturally in longitudinal and experimental work.


Asunto(s)
Red Social , Estudiantes , Adulto , Argentina , Femenino , Humanos , Masculino , España , Uruguay/epidemiología , Adulto Joven
2.
Mol Cell Endocrinol ; 559: 111777, 2023 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-36210601

RESUMEN

Loss-of-function mutations in melanocortin-4 receptor (MC4R) are the most common cause of monogenic obesity, a severe type of early-onset obesity. Our aim was to determine the prevalence of MC4R mutations in a cohort of 97 Argentinian children with early-onset obesity. We found two novel mutations (p.V52E and p.G233S) and estimated a prevalence of 2.1%. We investigated the pathogenicity of mutations in HEK293T cells expressing wild-type or mutant MC4R and found that both mutants exhibited reduced plasma membrane expression and altered agonist-induced cAMP responses, with no changes in basal activity. Besides, MC4R G233S mutant demonstrated an altered agonist-dependent inhibition of voltage-gated calcium channels type 2.2. Results using a Gαs protein inhibitor suggest that the G233S mutation could be recruiting a different G-protein signaling pathway. The identification of new mutations in MC4R and characterization of their functional impact provide tools for the diagnosis and treatment of monogenic obesity.


Asunto(s)
Obesidad Pediátrica , Receptor de Melanocortina Tipo 4 , Niño , Humanos , Estudios de Cohortes , Células HEK293 , Mutación , Receptor de Melanocortina Tipo 4/genética , Obesidad Pediátrica/genética , Argentina
3.
s.l; CONETEC; 1 nov. 2022.
No convencional en Español | BRISA/RedTESA, BRISA/RedTESA | ID: biblio-1400075

RESUMEN

INTRODUCCIÓN: La epidermólisis bullosa o ampollosa es un grupo heterogéneo de enfermedades mecanoampollosas genéticas autosómicas, dominantes o recesivas, caracterizadas por diversos grados de fragilidad de la piel y las mucosas causada por mutaciones que afectan las proteínas estructurales de la piel. Se han descripto cuatro tipos principales de epidermólisis bullosa, según el nivel estructural de división del tejido en la piel: epidermólisis bullosa simple, de la unión, distrófica y de Kindler. La gravedad de la enfermedad está determinada por el nivel de ampollas y el tipo de mutación y es muy variable entre los subtipos. La epidermólisis bullosa simple es autosómica dominante de separación intraepidérmica, con ampollas con trauma leve que curan sin dejar cicatriz y suele presentarse al nacer o en la primera infancia y de curso crónico, pero la formación de ampollas tiende a disminuir con la edad. La de la unión es autosómica recesiva de separación intra-lamina lucida, es la variante más grave, con muerte prematura en la infancia debido a la desnutrición o infecciones, y se caracteriza por ampollas y erosiones generalizadas con tejido de granulación que no cicatriza; asociado con uñas ausentes, dientes displásicos, lesiones orales y estenosis pilórica. La distrófica es autosómica recesiva y dominante de separación de láminas sub-basales y suele comenzar al nacer, con ampollas generalizadas que curan con cicatrices extensas que traen un riesgo de presentar secuelas graves y con muerte en la edad adulta temprana por las complicaciones. Finalmente, la Kindler es autosómica recesiva de separación a nivel de los queratinocitos basales o de lámina sub-basal, con ampollas inducidas por traumatismos leves que pueden disminuir con la edad. El manejo de los pacientes con epidermólisis bullosa es principalmente de apoyo e incluye el cuidado de heridas y la prevención y tratamiento de complicaciones (control del dolor, nutrición, cuidado de lesiones extra-cutáneas, etc.) involucrando a un equipo multidisciplinario de profesionales, donde en la actualidad no existe una terapia dirigida para la epidermólisis bullosa. TECNOLOGÍA: Oleogel-S10 (Filsuvez®) es un gel cicatrizante que contiene extracto seco de la corteza de Betulae (corteza de abedul), también conocido como extracto triterpénico. El gel contiene 90% peso/peso de aceite de girasol y 10% peso/peso de extracto seco de corteza de abedul, de los cuales la mayoría es betulina (72 a 88% peso/peso). Las sustancias marcadoras activas adicionales incluyen ácido betulínico, lupeol, ácido oleanólico y eritrodiol. OBJETIVO: El objetivo del presente informe es evaluar rápidamente los parámetros de eficacia, seguridad, costos y recomendaciones disponibles acerca del empleo de Filsuvez® gel (Oleogel-S10) para el tratamiento de la epidermólisis bullosa. MÉTODOS: Se realizó una búsqueda bibliográfica en las principales bases de datos tales como PUBMED, LILACS, BRISA, COCHRANE, SCIELO, EMBASE, TRIPDATABASE como así también en sociedades científicas, agencias reguladoras, financiadores de salud y agencias de evaluación de tecnologías sanitarias. Se priorizó la inclusión de revisiones sistemáticas, ensayos clínicos controlados aleatorizados, evaluación de tecnología sanitaria y guías de práctica clínica de alta calidad metodológica. En PubMed se utilizó la estrategia de búsqueda que se detalla en el Anexo I. La fecha de búsqueda de información fue hasta el 01 de noviembre de 2022. Para la búsqueda en Pubmed se utilizó la siguiente estrategia de búsqueda: ((Filsuvez[tiab] OR Oleogel-S10[tiab] OR birch bark extract[tiab]) AND (Epidermolysis Bullosa[MeSH] OR Acantholysis Bullosa[tiab])). RECOMENDACIONES: No se hallaron guías de práctica clínica actualizadas en Argentina y en el Mundo que mencionen la tecnología en la indicación evaluada. El Instituto Nacional para la Excelencia en Salud y Atención (NICE su sigla del inglés, National Institute for Health and Care Excellence) de Reino Unido y la Agencia Canadiense de Medicamentos y Tecnologías en Salud (CADTH su sigla del inglés, Canadian Agency for Drugs & Technologies in Health) no han evaluado al tratamiento en la indicación evaluada. CONCLUSIONES: La evidencia que sustenta la aprobación de comercialización de Oleogel-S10 (Filsuvez®) para el tratamiento de heridas de espesor parcial asociadas con epidermólisis bullosa en personas a partir de los 6 meses de edad, se basa en un estudio clínico con resultados aún no publicados con control de pares. Este estudio demostraría que en personas sin complicaciones, el OleogelS10 sumado al cuidado estándar aumentaría la proporción de personas con cierre completo de la herida en personas con epidermólisis bullosa distrófica de herencia recesiva frente a placebo al corto plazo. No se observarían beneficios en personas con epidermólisis bullosa de la unión o con distrófica de herencia dominante, como tampoco para otros desenlaces. Los eventos adversos reportados más comunes fueron las complicaciones de heridas, reacciones en el lugar de aplicación, infecciones de heridas, prurito y reacciones de hipersensibilidad. La agencia regulatoria de los Estados Unidos todavía no ha autorizado su comercialización; mientras que la agencia europea la ha autorizado recientemente, junto con la designación de medicamento huérfano, para el tratamiento de heridas de espesor parcial asociadas con epidermólisis bullosa distrófica y de la unión en personas a partir de los 6 meses de edad. No se hallaron guías de práctica clínica actualizadas en Argentina y en el Mundo que mencionen la tecnología en la indicación evaluada. No se hallaron evaluaciones económicas publicadas, como tampoco precios de adquisición de referencia en el Mundo.


Asunto(s)
Humanos , Triterpenos/uso terapéutico , Cicatrización de Heridas , Epidermólisis Ampollosa/tratamiento farmacológico , Betula/efectos de los fármacos , Argentina , Eficacia , Análisis Costo-Beneficio
4.
Aquat Toxicol ; 253: 106342, 2022 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-36327688

RESUMEN

The lower Salado River basin receive agricultural, industrial and domestic waste water. So, the aim was to evaluate the quality of three sampling sites that belong to the Salado River basin (S1: Cululú stream; S2: Salado River, at Esperanza City, S3: Salado River at Santo Tomé City) based on physicochemical parameters, metals and pesticides analyses and ecotoxicity on Rhinella arenarum larvae. R. arenarum larvae (Gosner Stage -GS- 25) were chronically exposed (504h) to complex matrixes of surface water and sediment samples of each site for the determination of the survival rate. Biomarkers of oxidative stress, neurotoxicity and genotoxicity were analyzed in R. arenarum larvae (GS. 25) after exposure (96h) to the complex matrix of water and sediment. The water quality index showed a marginal quality for all sites, influenced mainly by low dissolved oxygen, high total suspended solid, phosphate, nitrite, conductivity, Pb, Cr and Cu levels. Metal concentrations were higher in sediment than in water samples (˜34-35000 times). In total, thirty different pesticides were detected in all water and sediment samples, S1 presented the greatest variety (26). Glyphosate and AMPA were detected in sediments from all sites, being higher in S3. N,N-Diethyl-meta-toluamide (DEET) and atrazine were detected in all water samples. Greatest mortality was observed in larvae exposed to samples from S1 from 288h (43.3%), reaching a maximum value of 50% at 408h. Oxidative stress and genotoxicity were observed in larvae exposed to S1 and S3 matrix samples. Neurotoxicity was observed in larvae exposed to all matrix samples. The integrated biomarker response index showed that larvae exposed to S1 and S3 were the most affected. According to the physicochemical data and the ecotoxicity assessment, this important river basin is significantly degraded and may represent a risk to aquatic biota, especially for R. arenarum larvae.


Asunto(s)
Plaguicidas , Contaminantes Químicos del Agua , Animales , Ríos , Larva , Argentina , Contaminantes Químicos del Agua/toxicidad , Plaguicidas/análisis , Bufo arenarum , Metales/análisis , Monitoreo del Ambiente , Sedimentos Geológicos/análisis
5.
An Acad Bras Cienc ; 94(suppl 3): e20211430, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36417611

RESUMEN

Digeneans are common parasites of small mammals. Dicrocoeliidae is a family with a cosmopolitan distribution, with 18 genera previously recorded from mammals in the Americas, six of them parasitizing rodents in Argentina. In this study, an updated compilation is provided of Dicrocoeliidae from rodents in the Americas. Also, a new Platynosomoides species is described parasitizing the cricetid rodent Akodon montensis of the Atlantic Forest in Argentina. Digital repositories were used to search for Dicrocoeliidae from rodents in the Americas. Rodents were collected in four localities of the Atlantic Forest, Argentina. Digeneans were removed from the rodent's bile duct, and conventional studies were used for the morphological description. A total of 15 Dicrocoeliidae species were found parasitizing 18 rodent species from eight countries in the Americas. The new species of Platynosomoides from Akodon montensis differs from the other two species of genus by the size of body, testes, ovary, cecum length and position and length of the vitelline bands. Dicrocoeliidae show growing diversity, and the compilation of species in a rodent host base allows a clearer comparison and identification of new taxa in the future.


Asunto(s)
Dicrocoeliidae , Enfermedades de los Roedores , Trematodos , Animales , Femenino , Roedores , Argentina , Enfermedades de los Roedores/parasitología , Sigmodontinae , Arvicolinae
6.
JAMA ; 328(17): 1699-1700, 2022 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-36318122

RESUMEN

This Viewpoint discusses passage and implementation of Law 27.610, which legalized abortion in Argentina under certain circumstances, and examines the ongoing clinical issues and legal challenges to the law.


Asunto(s)
Aborto Criminal , Aborto Inducido , Femenino , Humanos , Embarazo , Aborto Inducido/legislación & jurisprudencia , Aborto Inducido/tendencias , Aborto Legal/legislación & jurisprudencia , Aborto Legal/tendencias , Argentina , Aborto Criminal/legislación & jurisprudencia , Aborto Criminal/tendencias
7.
Sci Rep ; 12(1): 19198, 2022 Nov 10.
Artículo en Inglés | MEDLINE | ID: mdl-36357519

RESUMEN

The exploitation of both conventional and unconventional hydrocarbons may lead to still not well-known environmental consequences such as ground deformation and induced/triggered seismicity. Identifying and characterizing these effects is fundamental for prevention or mitigation purposes, especially when they impact populated areas. Two case studies of such effects on hydrocarbon-producing basins in Argentina, the Neuquén and the Golfo de San Jorge, are presented in this work. The intense hydrocarbon production activities in recent years and their potential link with the occurrence of two earthquakes of magnitude 4.9 and 5 near the operating well fields is assessed. A joint analysis of satellite radar interferometry and records of fluid injection and extraction demonstrate that, between 2017 and 2020, vertical ground displacements occurred in both study areas over active well fields that might indicate a correlation to hydrocarbon production activities. Coseismic deformation models of the two earthquakes constrain source depths to less than 2 km. The absence of seismicity before the beginning of the hydrocarbon activities in both areas, and the occurrence of the two largest and shallow earthquakes in the vicinity of the active well fields just after intensive production periods, points towards the potential association between both phenomena.


Asunto(s)
Terremotos , Argentina , Hidrocarburos , Radar , Interferometría
8.
Trop Anim Health Prod ; 54(6): 371, 2022 Nov 03.
Artículo en Inglés | MEDLINE | ID: mdl-36326987

RESUMEN

The aim of this study was to evaluate some physiological parameters as total locomotor activity and subcutaneous temperature in two different bovine breeds under tropical environment. In this study, total locomotor activity and subcutaneous temperature were evaluated in 18 clinically healthy female cows. All cows were divided into 3 groups: group A1 was constituted by 6 Holstein Bos taurus, group A2 was constituted by 6 female Herford Bos taurus cows, and group B was constituted by 6 female Bradford Bos indicus cows. Data recording of total locomotor activity (TLA) and subcutaneous temperature (ST) was performed by means of a subcutaneous thermometer (Thermocron) and an actimeter ACTIWATCH® (Cambridge Neurotechnology Ltd.) based on accelerometer technologies equipped on all subjects to record TLA. At the same time, thermal and hygrometric recording were carried out, considering the subtropical climate of Santa Fe. The application of GLM for statistical analysis showed a significant effect (P < 0.05) on statistical model and time of the day on TLA and ST for all groups; no significative effects on animal parameters were found for ST and TLA except for group B. Circadian parameters have been evaluated according to the single cosinor procedure of ST that showed a diurnal daily rhythmicity for all investigated groups and TLA which is focused almost during the photophase for groups A1 and A2 and during scotophase for group B. Considering different species and breeds, and different environmental conditions, this study suggested that some subjects may be much more able to adapt themselves to environmental stress than others. During their evolution from Bos taurus, zebu cattle (Bos indicus) have acquired genes that confer thermotolerance. Subjects from Bos indicus breeds are better able to regulate body temperature in response to heat stress than Bos taurus subjects.


Asunto(s)
Adaptación Fisiológica , Locomoción , Bovinos , Animales , Femenino , Temperatura , Argentina
9.
Curr Microbiol ; 79(12): 392, 2022 Nov 03.
Artículo en Inglés | MEDLINE | ID: mdl-36329271

RESUMEN

Streptococcus agalactiae (GBS) is a colonizing agent in pregnant women, the main cause of invasive neonatal infections, and the reason of serious diseases in non-pregnant adults. Several virulence determinants are involved in the pathogenesis. These include capsular polysaccharide, surface-localized proteins, and toxins. Penicillin is considered the first choice antibiotic for the treatment and prophylaxis; erythromycin, clindamycin and fluoroquinolones are recommended alternatives for penicillin-allergic GBS carriers or patients. Our objective was to investigate the virulence genetic characteristics and the antimicrobial susceptibility of 162 GBS colonizing and infective isolates recovered in Argentina. Serotypes Ia and III were the most prevalent ones, followed by Ib, II, V, IV and non-typeable. In relation to the 13 virulence genes screened, cpsA, cylE, hylB, lmb, and scpB were the most prevalent and could be postulated as vaccine epitopes; bca, rib, bac, hvgA, spb1, PI, PI-2a, and PI-2b were detected in lesser frequencies. No significant association was found between serotypes or virulence genes and colonizing or infective isolates but, on the contrary, significant association was observed between some genes and the most prevalent serotypes, la and III. The cluster analysis showed 52 virulence profiles and, antimicrobial resistance tests, 16 profiles, some with up to 4 resistances. Tetracycline resistance was significantly associated with colonizing isolates. Genes tetM and ermB conferring resistance to tetracyclines and macrolides, respectively, were the most commonly identified. Our findings show that GBS colonizing and infective isolates circulating in Argentina share similar features in terms of serotype and virulence genes and show a high level of antimicrobial resistance.


Asunto(s)
Infecciones Estreptocócicas , Streptococcus agalactiae , Adulto , Recién Nacido , Humanos , Femenino , Embarazo , Streptococcus agalactiae/genética , Virulencia/genética , Antibacterianos/farmacología , Argentina , Farmacorresistencia Bacteriana/genética , Genotipo , Penicilinas , Pruebas de Sensibilidad Microbiana
10.
BMC Prim Care ; 23(1): 277, 2022 11 08.
Artículo en Inglés | MEDLINE | ID: mdl-36348310

RESUMEN

BACKGROUND: Chagas is a public health problem, especially in Latin America, exacerbated by migratory movements and increasing urbanization. Argentina is among the countries with the highest estimated prevalence in the region, with 1,500,000 people infected, with mother to child as the main mode of transmission. Vertical transmission has been significantly reduced by treating women of childbearing age; several guidelines in the region recommend treatment as a primary prevention strategy for the child and a secondary prevention strategy for women and their families. Despite recommendations, women of childbearing age are not always treated, and children do not receive timely diagnosis and treatment. The objective of this research was to design an implementation strategy to improve using Chagas guidelines focused on attending women of childbearing age and children at the primary healthcare level and pilot it in three primary health care centers in Argentina. METHODS: We conducted a pilot feasibility study using the Consolidated Framework for Implementation Research. A qualitative process evaluation was conducted using semi-structured interviews with health care providers and observations in primary health care centers. RESULTS: We developed a multifaceted implementation strategy including training, flowcharts and reminders, a register of suspected and confirmed cases, and the selection of a management facilitator. The pilot study took place between September 2019 and May 2020. The implementation level was heterogeneous and varied depending on the components, being the facilitating factors, the simplicity of the intervention, professionals' willingness to expand the indication of serologic tests, and staff commitment to the adoption of intervention components. The main barriers found were the change of authorities at the local level, some professionals´ reluctance to administer etiological treatment, staff shortages, lack of diagnostic supplies, and the health emergency caused by the COVID-19 pandemic. CONCLUSIONS: Behavioral change strategies should be applied to improve implementation to address some of the main barriers, including support actions offered by opinion leaders, medical experts, and local health authorities. Rapid diagnostic tests should be readily available to maintain behavior changes. We suggest further refinement of the strategy and its implementation in more centers to assess outcomes prospectively with a hybrid implementation research design.


Asunto(s)
COVID-19 , Pandemias , Niño , Femenino , Humanos , Proyectos Piloto , Estudios de Factibilidad , COVID-19/epidemiología , Argentina/epidemiología , Transmisión Vertical de Enfermedad Infecciosa , Atención Primaria de Salud
11.
Epidemiol Infect ; 150: e187, 2022 Nov 03.
Artículo en Inglés | MEDLINE | ID: mdl-36325837

RESUMEN

The SARS-CoV-2 Omicron variant has increased infectivity and immune escape compared with previous variants, and caused the surge of massive COVID-19 waves globally. Despite a vast majority (~90%) of the population of Santa Fe city, Argentina had been vaccinated and/or had been infected by SARS-CoV-2 when Omicron emerged, the epidemic wave that followed its arrival was by far the largest one experienced in the city. A serosurvey conducted prior to the arrival of Omicron allowed to assess the acquired humoral defences preceding the wave and to conduct a longitudinal study to provide individual-level real-world data linking antibody levels and protection against COVID-19 during the wave. A very large proportion of 1455 sampled individuals had immunological memory against COVID-19 at the arrival of Omicron (almost 90%), and about half (48.9%) had high anti-spike immunoglobulin G levels (>200 UI/ml). However, the antibody titres varied greatly among the participants, and such variability depended mainly on the vaccine platform received, on having had COVID-19 previously and on the number of days elapsed since last antigen exposure (vaccine shot or natural infection). A follow-up of 514 participants provided real-world evidence of antibody-mediated protection against COVID-19 during a period of high risk of exposure to an immune-escaping highly transmissible variant. Pre-wave antibody titres were strongly negatively associated with COVID-19 incidence and severity of symptoms during the wave. Also, receiving a vaccine shot during the follow-up period reduced the COVID-19 risk drastically (15-fold). These results highlight the importance of maintaining high defences through vaccination at times of high risk of exposure to immune-escaping variants.


Asunto(s)
COVID-19 , Humanos , COVID-19/epidemiología , Argentina/epidemiología , Estudios Longitudinales , SARS-CoV-2 , Inmunoglobulina G
12.
Artículo en Inglés | MEDLINE | ID: mdl-36429984

RESUMEN

The aim of this study is to describe the discordance between the self-perceived risk and actual risk of HIV among young men who have sex with men (YMSM) and its associated factors. An online, cross-sectional study was conducted with 405 men recruited from an Argentinian NGO in 2017. Risk discordance (RD) was defined as the expression of the underestimation of risk, that is, as a lower self-perception of HIV risk, as measured with the Perceived Risk of HIV Scale, than the current risk of HIV infection, as measured by the HIV Incidence Risk Index. Multivariate logistic regression models were used to analyze the associations between the RD and the explanatory variables. High HIV risk was detected in 251 (62%), while 106 (26.2%) showed high self-perceived risk. RD was found in 230 (56.8%) YMSM. The predictors that increased RD were consistent condom use with casual partners (aOR = 3.8 [CI 95:1.5-11.0]), the use of Growler to meet partners (aOR = 10.38 [CI 95:161-121.94]), frequenting gay bars (aOR = 1.9 [95% CI:1.1-3.5]) and using LSD (aOR = 5.44 [CI 95:1.32-30.29]). Underestimation of HIV risk in YMSM is associated with standard HIV risk behavior and modulated by psychosocial aspects. Thus, prevention campaigns aimed at YMSM should include these factors, even though clinical practice does not. Health professionals should reconsider adapting their instruments to measure the risk of HIV in YMSM. It is unknown what score should be used for targeting high-risk YMSM, so more research is needed to fill this gap. Further research is needed to assess what score should be used for targeting high-risk in YMSM.


Asunto(s)
Infecciones por VIH , Minorías Sexuales y de Género , Masculino , Humanos , Infecciones por VIH/prevención & control , Homosexualidad Masculina/psicología , Conducta Sexual/psicología , Estudios Transversales , Argentina/epidemiología
13.
Molecules ; 27(22)2022 Nov 09.
Artículo en Inglés | MEDLINE | ID: mdl-36431788

RESUMEN

The chemical composition of propolis of four species of stingless bees (SLBs) from Argentina was determined, and its antibacterial and anticancer activity was evaluated on selected types of microbes and cancer cell lines. Volatile secretions of all propolis samples are formed by 174 C2-C15 organic compounds, mainly mono- and sesquiterpenes and their derivatives. The chromatograms of ether extracts showed 287 peaks, of which 210 were identified. The most representative groups in the extracts of various propolis samples were diterpenoids (mainly resin acids), triterpenoids and phenolic compounds: long-chain alkenyl phenols, resorcinols and salicylates. The composition of both volatile and extractive compounds turned out to be species-specific; however, in both cases, the pairwise similarity of the propolis of Scaptotrigona postica and Tetragonisca fiebrigi versus that of Tetragona clavipes and Melipona quadrifasciata quadrifasciata was observed, which indicated the similarity of the preferences of the respective species when choosing plant sources of resin. The composition of the studied extracts completely lacked flavonoids and phenolcarboxylic acids, which are usually associated with the biological activity and medicinal properties of propolis. However, tests on selected microbial species and cancer cell lines showed such activity. All propolis samples tested against Paenibacillus larvae, two species of Bacillus and E. coli showed biofilm inhibition unrelated to the inhibition of bacterial growth, leading to a decrease in their pathogenicity. Testing the anticancer activity of ether extracts using five types of cell cultures showed that all four types of propolis studied inhibit the growth of cancer cells in a dose- and time-dependent manner. Propolis harvested by T. clavipes demonstrated the highest cytotoxicity on all tested cell lines.


Asunto(s)
Ascomicetos , Própolis , Abejas , Animales , Própolis/farmacología , Própolis/química , Escherichia coli , Argentina , Flavonoides/química , Resinas de Plantas , Éteres
14.
Arch Cardiol Mex ; 92(4): 476-483, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36413701

RESUMEN

OBJECTIVE: To analyze the Peruvian scientific production on Heart Failure (HF) in the period 2000-2020. METHODS: We performed an observational bibliometric study, in the Scopus and Scielo database, of documents published on heart failure by an author with Peruvian affiliation, between the years 2000 and 2020. We describe variables of scientific production, number of annual publications, documents, characteristics of the publications, and institutions. The collaborative networks were analyzed by building a network of nodes using the VOSViewer v1.6.5 software. RESULTS: A 236 publications were found in Scopus and 55 in Scielo. The largest number of documents were original articles followed by review articles. The journal "The Lancet" and "Revista Peruana de Medicina Experimental y Salud Pública" were those that had the highest number of publications. Thirty-one countries were registered that had at least 5 documents published with an author from Peru. Scientific collaboration was mainly with the United States and at the regional level with Argentina. 55 documents were published with only Peruvian authors. CONCLUSION: The scientific production in HF by Peruvian authors is in exponential growth. And the Universidad Peruana Cayetano Heredia/CRÓNICAS is the Peruvian institution with the most publications on it.


OBJETIVO: Analizar la producción científica peruana sobre insuficiencia cardiaca (IC) en el periodo 2000-2020. MATERIAL Y MÉTODOS: Se realizó un estudio observacional bibliométrico en la base de datos de Scopus y Scielo de documentos publicados sobre IC por algún autor con filiación peruana entre los años 2000 y 2020. Se analizaron de manera descriptiva las variables de producción científica, número de publicaciones anuales, documentos, características de las publicaciones e instituciones. Se analizaron las redes colaborativas, construyéndose una red de nodos utilizando el software VOSViewer v1.6.5. RESULTADOS: Se encontró 236 publicaciones en Scopus y 55 en Scielo. El mayor número de documentos fueron artículos originales seguido de artículos de revisión. The Lancet y la Revista Peruana de Medicina Experimental y Salud Pública fueron las que tuvieron el mayor número de publicaciones. Se registraron 31 países que tuvieron al menos cinco documentos publicados con un autor del Perú. La colaboración científica fue principalmente con EE.UU. y a nivel regional con Argentina. Se publicaron 55 documentos con solo autores peruanos. CONCLUSIONES: La producción científica en IC de autores peruanos se encuentra en crecimiento exponencial. Y es la Universidad Peruana Cayetano Heredia/Crónicas la institución peruana con más publicaciones sobre esta.


Asunto(s)
Insuficiencia Cardíaca , Edición , Humanos , Perú , Bibliometría , Argentina
15.
PLoS One ; 17(11): e0276977, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36417347

RESUMEN

OBJECTIVE: The aim was to analyze the spatial association and relative risk (RR) of leprosy cases diagnosed in southern Brazil and in the Argentinean province of Misiones during 2010 to 2016. METHODS: This ecological-type epidemiological study analyzed data from the Health Ministries of both countries. The analysis included frequency measures, spatial autocorrelation, RR cluster analysis and map construction. RESULTS: A hyperendemic occurrence was identified in all study regions, in the state of Paraná 71.2% of the municipalities were hyperendemic and in Misiones, Argentina 41.2%. The GI* statistical analysis showed clusters of high incidence rates in the state of Paraná and low-risk clusters in much of the state of Rio Grande do Sul, both in Brazil. The analysis indicated an area with RR equal to 3.87 - (p < .0001) when considering the entire territory and an RR of 2.80 - (p < .0001) excluding the state of Paraná, with the number of departments of Misiones, Argentina included in the risk clusters increasing significantly. CONCLUSIONS: The findings indicate a high probability of similar illness in adjacent areas, according to their relative position in space, as the occurrence of the disease is influenced by neighboring clusters.


Asunto(s)
Lepra , Mariposas Nocturnas , Animales , Brasil/epidemiología , Argentina/epidemiología , Lepra/epidemiología , Análisis Espacial
16.
BMJ ; 379: e073070, 2022 11 30.
Artículo en Inglés | MEDLINE | ID: mdl-36450402

RESUMEN

OBJECTIVE: To estimate the effectiveness of a two dose vaccine schedule (mRNA-1273, BNT162b2, and BBIBP-CorV) against SARS-CoV-2 infection and covid-19 related death and short term waning of immunity in children (3-11 years old) and adolescents (12-17 years old) during periods of delta and omicron variant predominance in Argentina. DESIGN: Test negative, case-control study. SETTING: Database of the National Surveillance System and the Nominalized Federal Vaccination Registry of Argentina. PARTICIPANTS: 844 460 children and adolescents without previous SARS-CoV-2 infection eligible to receive primary vaccination schedule who were tested for SARS-CoV-2 by polymerase chain reaction or rapid antigen test from September 2021 to April 2022. After matching with their corresponding controls, 139 321 (60.3%) of 231 181 cases remained for analysis. EXPOSURES: Two dose mRNA-1273, BNT162b2, and BBIBP-CorV vaccination schedule. MAIN OUTCOME MEASURES: SARS-CoV-2 infection and covid-19 related death. Conditional logistic regression was used to estimate the odds of SARS-CoV-2 infection among two dose vaccinated and unvaccinated participants. Vaccine effectiveness was estimated as (1-odds ratio)×100%. RESULTS: Estimated vaccine effectiveness against SARS-CoV-2 infection was 61.2% (95% confidence interval 56.4% to 65.5%) in children and 66.8% (63.9% to 69.5%) in adolescents during the delta dominant period and 15.9% (13.2% to 18.6%) and 26.0% (23.2% to 28.8%), respectively, when omicron was dominant. Vaccine effectiveness declined over time, especially during the omicron period, from 37.6% (34.2% to 40.8%) at 15-30 days after vaccination to 2.0% (1.8% to 5.6%) after ≥60 days in children and from 55.8% (52.4% to 59.0%) to 12.4% (8.6% to 16.1%) in adolescents.Vaccine effectiveness against death related to SARS-CoV-2 infection during omicron predominance was 66.9% (6.4% to 89.8%) in children and 97.6% (81.0% to 99.7%) in adolescents. CONCLUSIONS: Vaccine effectiveness in preventing mortality remained high in children and adolescents regardless of the circulating variant. Vaccine effectiveness in preventing SARS-CoV-2 infection in the short term after vaccination was lower during omicron predominance and decreasing sharply over time. TRIAL REGISTRATION: National Registry of Health Research IS003720.


Asunto(s)
COVID-19 , Vacunas , Adolescente , Niño , Humanos , Preescolar , SARS-CoV-2 , COVID-19/epidemiología , COVID-19/prevención & control , Vacuna BNT162 , Vacuna nCoV-2019 mRNA-1273 , Estudios de Casos y Controles , Argentina/epidemiología
17.
An Acad Bras Cienc ; 94(suppl 4): e20200388, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36449854

RESUMEN

Homonota taragui is an endemic gecko of the northeast of Argentina. We estimate demographic parameters: number of individuals by populations, sex and ontogenetic stage; sexual dimorphism; survival of this species; and describe the use of microhabitat and movement patterns. We measured 11 morphological variables, determined the sex and marked individually. We found bigger head width in males. Some factors contribute to the vulnerability of this species such as its constrained geographical distribution, use of the specific microhabitat and the isolation of their populations. We highlight demography studies as an essential data source for management and conservation of critically endangered species.


Asunto(s)
Lagartos , Humanos , Masculino , Animales , Argentina , Ecología , Especies en Peligro de Extinción , Caracteres Sexuales
18.
An Acad Bras Cienc ; 94(4): e20200442, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36449891

RESUMEN

Acoustic communication plays a key role in the life of birds and it is useful in phylogenetic and evolutionary investigations. This study described the structure and function of Polioptila dumicola´s call repertoire in riparian environments from the Mid-eastern of Argentina. Male calls (N=644) were recorded in the field during the pairs nuptial interactions; nests exploration and previously capture of male. Then, specific function was assigned to different calls using standardized methods of acoustic classification and behavioral observations. Twelve different types of calls were classified. Alert and call to female were the most frequent calls and they represented 12.04% and 10.24% of recordings. Anguish call was also recorded in this species. These are the first results regarding to the calls and repertoire of P. dumicola. These data could be used for new studies on environmental selection pressures and for conservation of this species and its habitats.


Asunto(s)
Evolución Biológica , Comunicación , Femenino , Masculino , Humanos , Argentina , Filogenia
19.
BMC Plant Biol ; 22(1): 543, 2022 Nov 24.
Artículo en Inglés | MEDLINE | ID: mdl-36434507

RESUMEN

BACKGROUND: Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of the wheat crop. It causes significant reductions in both grain yield and grain quality. In recent years, new and more virulent races have overcome many of the known resistance genes in Argentinian germplasm. In order to identify loci conferring resistance to the local races of Pst for effective utilization in future breeding programs, a genome-wide association study (GWAS) was performed using a collection of 245 bread wheat lines genotyped with 90 K SNPs. RESULTS: To search for adult plant resistance (APR) the panel was evaluated for disease severity (DS) and area under disease progress curve (AUDPC) in field trials during two years under natural infection conditions. To look for seedling or all-stage resistance (ASR) the panel was evaluated to determine infection type (IT) under greenhouse conditions against two prevalent races in Argentina. The phenotypic data showed that the panel possessed enough genetic variability for searching for sources of resistance to Pst. Significant correlations between years were observed for Pst response in the field and high heritability values were found for DS (H2 = 0.89) and AUDPC (H2 = 0.93). Based on GWAS, eight markers associated with Pst resistance (FDR < 0.01) were identified, of these, five were associated with ASR (on chromosomes 1B, 2A, 3A and 5B) and three with APR (on chromosomes 3B and 7A). These markers explained between 2% and 32.62% of the phenotypic variation. Five of the markers corresponded with previously reported Yr genes/QTL, while the other three (QYr.Bce.1B.sd.1, QYr.Bce.3A.sd and QYr.Bce.3B.APR.2) might be novel resistance loci. CONCLUSION: Our results revealed high genetic variation for resistance to Argentinian stripe rust races in the germplasm used here. It constitutes a very promising step towards the improvement of Pst resistance of bread wheat in Argentina. Also, the identification of new resistance loci would represent a substantial advance for diversifying the current set of resistance genes and to advance in the improvement of the durable resistance to the disease.


Asunto(s)
Basidiomycota , Triticum , Triticum/genética , Estudio de Asociación del Genoma Completo , Pan , Resistencia a la Enfermedad/genética , Sitios de Carácter Cuantitativo , Argentina , Enfermedades de las Plantas/genética , Fitomejoramiento , Basidiomycota/fisiología
20.
J Vet Sci ; 23(6): e12, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-36448431

RESUMEN

BACKGROUND: Staphylococcus aureus and Streptococcus agalactiae are the main cause of clinical mastitis in dairy cattle in Argentina, whereas coagulase-negative staphylococci (CNS) and environmental streptococci are the main cause of subclinical mastitis. Bacteria isolated from infected animals show increasing antimicrobial resistance. OBJECTIVES: This study aims to determine the antimicrobial resistance of staphylococci and streptococci isolated from milk with mastitis, and to genotypically characterize the methicillin-resistant (MR) staphylococci. METHODS: Isolation was performed on blood agar and identification was based on biochemical reactions. Antimicrobial susceptibility was according to the Clinical and Laboratory Standards Institute guidelines. The antimicrobial resistance genes, SCCmec type and spa type were detected by the polymerase chain reaction method. RESULTS: We isolated a total of 185 staphylococci and 28 streptococci from 148 milk samples. Among the staphylococcal isolates, 154 were identified as CNS and 31 as S. aureus. Among the 154 CNS, 24.6% (n = 38) were resistant to penicillin, 14.9% (n = 23) to erythromycin, 17.5% (n = 27) to clindamycin, 6.5% (n = 10) to cefoxitin and oxacillin. Among the S. aureus isolates, 16.1% (n = 5) were resistant to penicillin, 3.2% (n = 1) to cefoxitin and oxacillin (MRSA). Six MR isolates (5 CNS and 1 MRSA) were positive to the mecA gene, and presented the SCCmec IVa. The MRSA strain presented the sequence type 83 and the spa type 002. Among the 28 streptococcal isolates, 14.3% (n = 4) were resistant to penicillin, 10.7% (n = 3) to erythromycin and 14.3% (n = 4) to clindamycin. CONCLUSIONS: The present findings of this study indicate a development of antimicrobial resistance in main bacteria isolated from cows with mastitis in Argentina.


Asunto(s)
Enfermedades de los Bovinos , Mastitis Bovina , Femenino , Bovinos , Animales , Staphylococcus , Antibacterianos/farmacología , Staphylococcus aureus , Cefoxitina , Clindamicina , Argentina/epidemiología , Farmacorresistencia Bacteriana , Mastitis Bovina/epidemiología , Streptococcus , Oxacilina , Eritromicina
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