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1.
Ann R Coll Surg Engl ; 102(8): e213-e215, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-32588651

RESUMEN

Synovial chondromatosis is a rare benign condition. It most commonly affects the large joints. Presentation in the temporomandibular joint is rare. Our case was an incidental radiological finding and not diagnosed immediately, highlighting the ease with which conditions such as this can be missed, particularly in asymptomatic patients. Only 45% of patients with synovial chondromatosis show radiographic changes. Findings as significant as ours are unusual. An increased professional awareness of the radiological signs of synovial chondromatosis would be beneficial to improve diagnosis and prognosis for patients.


Asunto(s)
Condromatosis Sinovial , Articulación Temporomandibular , Humanos , Masculino , Persona de Mediana Edad , Articulación Temporomandibular/diagnóstico por imagen , Articulación Temporomandibular/patología , Articulación Temporomandibular/cirugía
2.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(5): 343-347, 2020 May 09.
Artículo en Chino | MEDLINE | ID: mdl-32392978

RESUMEN

Objective: To observe the effect of autophagy of condylar chondrocytes on apoptosis in temporomandibular joint osteoarthritis (TMJOA) of rats. Methods: Fourty male 2-month-old SPF SD rats were equally divided into sham group (n=20) and experimental group (n=20). UAC metal prosthesis was cemented to the left incisors of maxilla and mandible of the rats in experimental group rats. After 8 weeks, all rats were sacrificed and the temporomandibular joint was taken. Two groups of rat condylar chondrocytes were extracted and cultured in vitro to the third generation. Immunofluorescence technique was used to detect the levels of collagen Ⅱ and matrix metalloproteinase-13 (MMP-13) in chondrocytes. The level of light chain-3 (LC-3), an autophagy marker of chondrocytes, was detected. Immunohistochemical technique was used to detect the level glycogenin-1, a glycogen formation marker of chondrocyte, was detected. The level of caspase-3, an apoptosis marker of chondrocyte, was also detected. Tunel technique was used to detect the apoptosis rate of the two groups at 72 h. Cracking cell extraction of total protein, Western-blotting (WB) technology to detect the levels of collagen Ⅱ, MMP -13, LC-3, glycogenin-1, caspase-3 and make gray analysis. Results: Compared with sham group, the level of collagen Ⅱ decreased, MMP-13 increased, LC-3 decreased, glycogenin-1 increased and caspase-3 increased in experimental group. The apoptosis rate of chondrocytes in experimentaal group [ (17.3±4.4) %] at 72h was higher than that in control group [ (5.6±2.1) %](t=10.732, P<0.001) .WB bands gray statistical results show that the level of collagen Ⅱ in chondrocytes of experimental group (0.43±0.21) was lower than that of control group (0.71±0.26) (t=2.409, P=0.043) , the level of MMP-13 in chondrocytes of experimental group (0.73±0.31) was higher than that of control group (0.24±0.10) (t=3.364, P=0.010) , the level of LC-3 in chondrocytes of experimental group (0.09±0.04) was lower than that of control group (0.39±0.18) (t=3.638, P=0.007) , the level of glycogenin-1 in chondrocytes of experimental group (0.68±0.30) was higher than that of control group (0.29±0.17) (t=2.529, P=0.035) , the level of caspase-3 in chondrocytes of experimental group (0.19±0.08) was higher than that of control group (0.05±0.02) (t=3.796, P=0.005) . Conclusions: The level of autophagy of condylar chondrocytes in temporomandibular joint of rats decreased, glycogen accumulation increased, the rate of chondrocyte apoptosis increased, and the number of chondrocytes decreased, resulting in degeneration of condylar cartilage tissue.


Asunto(s)
Apoptosis , Autofagia , Condrocitos/citología , Osteoartritis/patología , Articulación Temporomandibular/patología , Animales , Cartílago Articular/citología , Implantes Dentales , Glucógeno/análisis , Masculino , Ratas , Ratas Sprague-Dawley , Articulación Temporomandibular/citología
3.
PLoS One ; 15(4): e0231734, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32294140

RESUMEN

Osteoarthritis is a degenerative disease that causes substantial changes in joint tissues, such as cartilage degeneration and subchondral bone sclerosis. Chondroitin sulfate and glucosamine are commonly used products for the symptomatic treatment of osteoarthritis. The aim of the present study was to investigate the effects of these products when used as structure-modifying drugs on the progression of osteoarthritis in the rabbit temporomandibular joint. Thirty-six New Zealand rabbits were divided into 3 groups (n = 12/group): control (no disease); osteoarthritis (disease induction); and treatment (disease induction and administration of chondroitin sulfate and glucosamine). Osteoarthritis was induced by intra-articular injection of monosodium iodoacetate. Animals were killed at 30 and 90 days after initiation of therapy. The treatment was effective in reducing disease severity, with late effects and changes in the concentration of glycosaminoglycans in the articular disc. The results indicate that chondroitin sulfate and glucosamine may have a structure-modifying effect on the tissues of rabbit temporomandibular joints altered by osteoarthritis.


Asunto(s)
Artritis Experimental/tratamiento farmacológico , Sulfatos de Condroitina/administración & dosificación , Glucosamina/administración & dosificación , Osteoartritis/tratamiento farmacológico , Articulación Temporomandibular/efectos de los fármacos , Animales , Artritis Experimental/inducido químicamente , Artritis Experimental/diagnóstico , Artritis Experimental/patología , Cartílago Articular/citología , Cartílago Articular/efectos de los fármacos , Cartílago Articular/patología , Modelos Animales de Enfermedad , Quimioterapia Combinada/métodos , Matriz Extracelular/efectos de los fármacos , Matriz Extracelular/patología , Humanos , Inyecciones Intraarticulares , Inyecciones Subcutáneas , Ácido Yodoacético/administración & dosificación , Ácido Yodoacético/toxicidad , Masculino , Osteoartritis/inducido químicamente , Osteoartritis/diagnóstico , Osteoartritis/patología , Conejos , Índice de Severidad de la Enfermedad , Articulación Temporomandibular/patología
4.
Cell Prolif ; 53(4): e12755, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32154622

RESUMEN

OBJECTIVES: Ageing could be a contributing factor to the progression of temporomandibular joint osteoarthritis (TMJ OA), whereas its pathogenesis and potential therapeutic strategy have not been comprehensively investigated. MATERIALS AND METHODS: We generated ageing mouse models (45-week and 60-week; 12-week mice as control) and intermittently injected 45-week mice with parathyroid hormone (PTH(1-34)) or vehicle for 4 weeks. Cartilage and subchondral bone of TMJ were analysed by microCT, histological and immunostaining. Western blot, qRT-PCR, ChIP, ELISA and immunohistochemical analysis were utilized to examination the mechanism of PTH(1-34)'s function. RESULTS: We showed apparent OA-like phenotypes in ageing mice. PTH treatment could ameliorate the degenerative changes and improve bone microarchitecture in the subchondral bone by activating bone remodelling. Moreover, PTH inhibited phosphorylation level of Smad3, which can combine with p16ink4a gene promoter region, resulting in reduced senescent cells accumulation and increased cellular proliferation of marrow mesenchymal stem cells (MSCs). ELISA also showed relieved levels of specific senescent-associated secretory phenotype (SASP) in ageing mice after PTH treatment. CONCLUSIONS: In summary, PTH may reduce the accumulation of senescent cells in subchondral bone by inhibiting p16ink4a and improve bone marrow microenvironment to active bone remodelling process, indicating PTH administration could be a potential preventative and therapeutic treatment for age-related TMJ OA.


Asunto(s)
Hormonas y Agentes Reguladores de Calcio/uso terapéutico , Osteoartritis/tratamiento farmacológico , Hormona Paratiroidea/uso terapéutico , Articulación Temporomandibular/efectos de los fármacos , Envejecimiento , Animales , Células Cultivadas , Modelos Animales de Enfermedad , Masculino , Ratones , Ratones Endogámicos C57BL , Osteoartritis/patología , Osteoartritis/fisiopatología , Osteogénesis/efectos de los fármacos , Articulación Temporomandibular/patología , Articulación Temporomandibular/fisiopatología
5.
Int. arch. otorhinolaryngol. (Impr.) ; 24(1): 68-72, Jan.-Mar. 2020. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1090546

RESUMEN

Abstract Introduction The prevalence of tinnitus is higher in individuals with temporoman- dibular joint disorder (TMD) than in the general population. Magnetic resonance imaging (MRI) of the temporomandibular joint (TMJ) is the method of choice for investigation, and it has been hypothesized that specific MRI findings might be observed in TMD with comorbid tinnitus. Objective To comparatively describe MRI findings in patients with TMD with and without tinnitus, identifying the most common TMJ alterations and determining whether a correlation exists between severity of TMD and tinnitus. Methods A cross-sectional study of 53 adult patients with bilateral or unilateral TMD (30 with and 23 without tinnitus). The association between tinnitus and morphological aspects of TMD (changes in condylar morphology, articular eminence morphology, and disc morphology), disc displacement (with/without reduction), condylar translation, and intra-articular effusion was analyzed on MRI images. Results The mean patient age was 46.12 ± 16.1 years. Disc displacement was the most common finding in both groups (24 patients with tinnitus versus 15 without; p = 0.043). Only the frequency of disc displacement with reduction was significantly different between groups. Conclusion Additional imaging techniques should be explored to detect specific aspects of the relationship between tinnitus and TMD.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Articulación Temporomandibular/diagnóstico por imagen , Acúfeno/diagnóstico por imagen , Imagen por Resonancia Magnética , Trastornos de la Articulación Temporomandibular/diagnóstico por imagen , Audiometría de Respuesta Evocada , Audiometría de Tonos Puros , Articulación Temporomandibular/patología , Acúfeno/diagnóstico , Acúfeno/etiología , Índice de Severidad de la Enfermedad , Trastornos de la Articulación Temporomandibular/complicaciones , Trastornos de la Articulación Temporomandibular/patología , Estudios Transversales
6.
Int J Mol Med ; 45(2): 607-614, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31894302

RESUMEN

The morbidity of temporomandibular joint osteoarthritis (TMJOA) increases with age. Condylar articular cartilage degradation, which causes TMJOA, is known to be involved in articular chondrocyte metabolic imbalances in the temporomandibular joint (TMJ) and in other joints of the body. Epigenetic regulation, such as the chemical modification of DNA and histones, is implicated in cartilage homeostasis. However, few studies have been conducted on the epigenetic regulation of condylar articular cartilage degradation. The present study investigated the regulation of histone H3 lysine 9 (H3K9) methylation and its effects on the pathogenesis of degenerative TMJ cartilage disorders. The histone H3K9 methylation level was decreased in degenerated condylar articular cartilage in aged mice. Treatment with chaetocin (a selective H3K9 methylation inhibitor) reduced cell viability and promoted caspase­3/7 activity in ATDC5 mouse chondroprogenitor cells. The inhibition of H3K9 methylation increased matrix metalloproteinase (Mmp)1 and Mmp13 mRNA expression in these cells. Furthermore, the expression levels of Sox9 and collagen α1(II) (Col2a1) mRNA, which are anabolic factors for chondrogenic differentiation, were also decreased by treatment with chaetocin, which is an inhibitor of histone methyltransferases. These results indicated that histone H3K9 methylation regulates chondrocyte homeostasis in terms of cell growth, apoptosis and gene expression, and highlighted a possible future therapy option for TMJOA.


Asunto(s)
Histonas/metabolismo , Osteoartritis/patología , Articulación Temporomandibular/patología , Envejecimiento , Animales , Apoptosis , Proliferación Celular , Femenino , Lisina/metabolismo , Metilación , Ratones , Ratones Endogámicos C57BL , Osteoartritis/metabolismo , Articulación Temporomandibular/metabolismo
7.
Vet Radiol Ultrasound ; 61(3): 346-352, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31896164

RESUMEN

Equine temporomandibular joint (TMJ) diseases are increasingly recognized as a problem for the well-being and performance of horses. Diagnosis is confounded by overlap of clinical signs associated with pathology of the oral cavity, poll, and cervical vertebrae. Arthrocentesis for intra-articular analgesia, sampling of synovial fluid, and medication is needed for diagnostic and therapeutic purposes. Ultrasound features of the normal TMJ and a blind arthrocentesis technique have been described, but a systematic approach to ultrasound-guided (USG) arthrocentesis has not been reported. Ultrasound guidance allows visualization of the TMJ that may prove beneficial in cases when pathology, abnormal anatomy, or clinician inexperience make blind arthrocentesis difficult. We hypothesized that USG arthrocentesis would result in fewer needle repositions than blind arthrocentesis. We also aimed to assess synovial fluid parameters for normal equine TMJs. A prospective randomized method comparison with crossover experimental design compared the number of needle positionings required for accurate injection of the TMJ using each technique. Arthrocentesis technique and operator experience were tested using cadavers and two operators. Injection success was confirmed using CT. The radiologist then applied both techniques in normal live horses. No statistically significant difference was noted between arthrocentesis techniques or operators (P > .05). No complications were observed in live horses following either technique. Synovial fluid parameters were largely within the normal range expected for other synovial joints. Either blind or USG arthrocentesis of the equine TMJ can be performed with minimal prior operator experience. Ultrasound-guided arthrocentesis is an alternative method and can be considered in cases with altered anatomy.


Asunto(s)
Artrocentesis/veterinaria , Enfermedades de los Caballos/diagnóstico por imagen , Trastornos de la Articulación Temporomandibular/veterinaria , Articulación Temporomandibular/diagnóstico por imagen , Ultrasonografía Intervencional/veterinaria , Animales , Artrocentesis/métodos , Cadáver , Enfermedades de los Caballos/patología , Caballos , Estudios Prospectivos , Líquido Sinovial , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/diagnóstico por imagen , Trastornos de la Articulación Temporomandibular/terapia
8.
In Vivo ; 34(1): 95-99, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31882467

RESUMEN

BACKGROUND/AIM: Few studies have performed magnetic resonance (MR) imaging on live animals. The aim of this study was to perform 7T MR microimaging of the temporomandibular joint (TMJ) multiple times in the same living mice with malocclusion, and to compare between MR imaging and histopathological findings. MATERIALS AND METHODS: Mice were examined by MR imaging at 3-4, 6 and 12 weeks following the attachment of a metal tube on the left mandibular incisor. Histopathological examination was done at 3, 6 and 12 weeks. RESULTS: The detailed structure of the TMJ was evident from MR microimaging. The histopathological examination showed some changes in the cartilage, but no changes in the bone structure of these mice. CONCLUSION: We successfully performed multiple 7T MR imaging in living mice. Even if the TMJ showed no obvious changes on MR images, minute changes may be present in the cartilage.


Asunto(s)
Articulación Temporomandibular/patología , Animales , Cartílago Articular/patología , Modelos Animales de Enfermedad , Imagen por Resonancia Magnética/métodos , Masculino , Maloclusión/patología , Ratones , Ratones Endogámicos C57BL
9.
Oral Maxillofac Surg Clin North Am ; 32(1): 117-134, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31699581

RESUMEN

Temporomandibular joint (TMJ) arthritis impacts mandibular growth and development. This can result in skeletal deformity, such as facial asymmetry and/or malocclusion asymmetry. This article reviews the unique properties of TMJ and dentofacial growth and development in the setting of juvenile idiopathic arthritis (JIA). Specific orthopedic/orthodontic and surgical management of children with JIA and TMJ arthritis is discussed. The importance of interdisciplinary collaboration is highlighted.


Asunto(s)
Artritis Juvenil/complicaciones , Deformidades Dentofaciales , Maloclusión , Procedimientos Quirúrgicos Ortognáticos/métodos , Trastornos de la Articulación Temporomandibular/complicaciones , Articulación Temporomandibular/patología , Niño , Humanos , Desarrollo Maxilofacial
10.
Am J Vet Res ; 80(12): 1107-1113, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31763947

RESUMEN

OBJECTIVE: To describe histologic changes in the temporomandibular joints (TMJs) of horses of various ages. SAMPLE: 22 TMJs from cadavers of 11 horses. PROCEDURES: Horses were categorized into 3 age groups (group 1, 2 to 10 years old [n = 3]; group 2, 11 to 20 years old [3]; and group 3, > 20 years old [5]). Each TMJ was sectioned into 5-mm slices, preserved in formalin, decalcified in formic acid, and routinely processed for histologic analysis. Joints were systematically assessed by use of previously described methods. Multilevel mixed-effects models were used to examine the data. RESULTS: The number of changes was significantly fewer and degree of changes was significantly less within the TMJs of group 1 horses, compared with those of group 3 horses. Comparison among groups revealed that the combination of temporal and mandibular scores for group 1 was significantly lower than for groups 2 or 3. Disk score did not differ significantly between groups 1 and 2, but disk scores of groups 1 and 2 were significantly lower than the disk score of group 3. CONCLUSIONS AND CLINICAL RELEVANCE: The assessed lesions were associated with osteoarthritis, and they accumulated in the TMJs as horses aged. In the absence of signs of pain manifested as changes in mastication, behavior, or performance, it would be difficult to determine the point at which accrued pathological changes represented the onset of clinically important osteoarthritis of the TMJs.


Asunto(s)
Caballos , Osteoartritis/veterinaria , Trastornos de la Articulación Temporomandibular/patología , Articulación Temporomandibular/patología , Factores de Edad , Animales , Femenino , Masculino , Osteoartritis/patología , Articulación Temporomandibular/anatomía & histología
11.
PLoS One ; 14(10): e0223244, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31603905

RESUMEN

The temporomandibular joint (TMJ) is a fibrocartilaginous tissue critical for chewing and speaking. In patients with temporomandibular disorders (TMDs), permanent tissue loss can occur. Recapitulating the complexity of TMDs in animal models is difficult, yet critical for the advent of new therapies. Synovial fluid from diseased human samples revealed elevated levels of tumor necrosis factor alpha (TNF-alpha). Here, we propose to recapitulate these findings in mice by subjecting murine TMJs with TNF-alpha or CFA (Complete Freund's Adjuvant) in mandibular condyle explant cultures and by local delivery in vivo using TMJ intra-articular injections. Both TNF-alpha and CFA delivery to whole mandibular explants and in vivo increased extracellular matrix deposition and increased cartilage thickness, while TNF-alpha treated explants had increased expression of inflammatory cytokines and degradative enzymes. Moreover, the application of TNF-alpha or CFA in both models reduced cell number. CFA delivery in vivo caused soft tissue inflammation, including pannus formation. Our work provides two methods of chemically induced TMJ inflammatory arthritis through a condyle explant model and intra-articular injection model that replicate findings seen in synovial fluid of human patients, which can be used for further studies delineating the mechanisms underlying TMJ pathology.


Asunto(s)
Artritis Experimental/inmunología , Cartílago Articular/inmunología , Matriz Extracelular/inmunología , Trastornos de la Articulación Temporomandibular/inmunología , Articulación Temporomandibular/inmunología , Proteína ADAMTS5/genética , Proteína ADAMTS5/inmunología , Adolescente , Adulto , Anciano , Animales , Artritis Experimental/inducido químicamente , Artritis Experimental/genética , Cartílago Articular/efectos de los fármacos , Cartílago Articular/patología , Colágeno Tipo II/genética , Colágeno Tipo II/inmunología , Colágeno Tipo X/genética , Colágeno Tipo X/inmunología , Modelos Animales de Enfermedad , Matriz Extracelular/efectos de los fármacos , Matriz Extracelular/patología , Femenino , Adyuvante de Freund/administración & dosificación , Expresión Génica/efectos de los fármacos , Expresión Génica/inmunología , Humanos , Interleucinas/genética , Interleucinas/inmunología , Masculino , Cóndilo Mandibular/efectos de los fármacos , Cóndilo Mandibular/inmunología , Cóndilo Mandibular/patología , Ratones , Ratones Endogámicos C57BL , Persona de Mediana Edad , Líquido Sinovial/inmunología , Articulación Temporomandibular/efectos de los fármacos , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/genética , Trastornos de la Articulación Temporomandibular/patología , Técnicas de Cultivo de Tejidos , Factor de Necrosis Tumoral alfa/administración & dosificación
13.
Inflamm Res ; 68(10): 889-900, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31372663

RESUMEN

OBJECTIVE: To investigate the participation of canonical Wnt and NF-κB signaling pathways in an experimental model of chronic arthritis induced by methylated bovine serum albumin (mBSA) in rat temporomandibular joint (TMJ). MATERIALS AND METHODS: Wistar rats were sensitized by mBSA+Complete Freund Adjuvant (CFA)/Incomplete Freund Adjuvant (IFA) on the first 14 days (1 ×/week). Subsequently, they received 1, 2 or 3 mBSA or saline solution injections into the TMJ (1 ×/week). Hypernociceptive threshold was assessed during the whole experimental period. 24 h after the mBSA injections, the TMJs were removed for histopathological and immunohistochemical analyses for TNF-α, IL-1ß, NF-κB, RANKL, Wnt-10b, ß-catenin and DKK1. RESULTS: The nociceptive threshold was significantly reduced after mBSA injections. An inflammatory infiltrate and thickening of the synovial membrane were observed only after mBSA booster injections. Immunolabeling of TNF-α, IL-1ß and Wnt-10b was increased in the synovial membrane in arthritic groups. The immunoexpression of nuclear ß-catenin was significantly higher only in the group that received 2 booster TMJ injections. However, NF-κB, RANKL and DKK1 immunoexpression were increased only in animals with 3 mBSA intra-articular injections. CONCLUSION: Our results suggest that canonical Wnt and NF-κB signaling pathways participate in the hypernociception and inflammatory response in TMJ synovial membrane during the development of rheumatoid arthritis in rats.


Asunto(s)
Artritis Experimental/inmunología , Artritis Reumatoide/inmunología , Hiperalgesia/inmunología , FN-kappa B/inmunología , Articulación Temporomandibular/inmunología , Vía de Señalización Wnt , Animales , Artritis Experimental/patología , Artritis Reumatoide/patología , Adyuvante de Freund , Hiperalgesia/patología , Péptidos y Proteínas de Señalización Intercelular/inmunología , Interleucina-1beta/inmunología , Lípidos , Masculino , Ligando RANK/inmunología , Ratas Wistar , Albúmina Sérica Bovina , Membrana Sinovial/inmunología , Membrana Sinovial/patología , Articulación Temporomandibular/patología , Factor de Necrosis Tumoral alfa/inmunología , Proteínas Wnt/inmunología
14.
Pan Afr Med J ; 32: 151, 2019.
Artículo en Francés | MEDLINE | ID: mdl-31303922

RESUMEN

Ankylosis of the temporomandibular joint (TMJ) is a joint stiffness with an oral aperture of less than 30 mm measured between the incisors, occurring because of a bony, fibrous or fibro-osseous fusion. Arthrosis is a rare cause of the ankylosis of the temporomandibular joint. We report a case of ankylosis of the TMJ due to osteoarthrosis, in order to highlight the diagnostic and therapeutic features of this quite uncommon disease.


Asunto(s)
Anquilosis/diagnóstico , Osteoartritis/diagnóstico , Trastornos de la Articulación Temporomandibular/diagnóstico , Anquilosis/patología , Femenino , Humanos , Persona de Mediana Edad , Osteoartritis/patología , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/patología
16.
Int J Mol Sci ; 20(9)2019 May 07.
Artículo en Inglés | MEDLINE | ID: mdl-31067826

RESUMEN

The temporomandibular joint (TMJ), which differs anatomically and biochemically from hyaline cartilage-covered joints, is an under-recognized joint in arthritic disease, even though TMJ damage can have deleterious effects on physical appearance, pain and function. Here, we analyzed the effect of IL-1ß, a cytokine highly expressed in arthritic joints, on TMJ fibrocartilage-derived cells, and we investigated the modulatory effect of mechanical loading on IL-1ß-induced expression of catabolic enzymes. TMJ cartilage degradation was analyzed in 8-11-week-old mice deficient for IL-1 receptor antagonist (IL-1RA-/-) and wild-type controls. Cells were isolated from the juvenile porcine condyle, fossa, and disc, grown in agarose gels, and subjected to IL-1ß (0.1-10 ng/mL) for 6 or 24 h. Expression of catabolic enzymes (ADAMTS and MMPs) was quantified by RT-qPCR and immunohistochemistry. Porcine condylar cells were stimulated with IL-1ß for 12 h with IL-1ß, followed by 8 h of 6% dynamic mechanical (tensile) strain, and gene expression of MMPs was quantified. Early signs of condylar cartilage damage were apparent in IL-1RA-/- mice. In porcine cells, IL-1ß strongly increased expression of the aggrecanases ADAMTS4 and ADAMTS5 by fibrochondrocytes from the fossa (13-fold and 7-fold) and enhanced the number of MMP-13 protein-expressing condylar cells (8-fold). Mechanical loading significantly lowered (3-fold) IL-1ß-induced MMP-13 gene expression by condylar fibrochondrocytes. IL-1ß induces TMJ condylar cartilage damage, possibly by enhancing MMP-13 production. Mechanical loading reduces IL-1ß-induced MMP-13 gene expression, suggesting that mechanical stimuli may prevent cartilage damage of the TMJ in arthritic patients.


Asunto(s)
Artritis Juvenil/metabolismo , Condrocitos/efectos de los fármacos , Interleucina-1beta/farmacología , Cóndilo Mandibular/metabolismo , Metaloproteinasa 13 de la Matriz/genética , Articulación Temporomandibular/metabolismo , Proteína ADAMTS4 , Proteína ADAMTS5/genética , Proteína ADAMTS5/metabolismo , Animales , Células Cultivadas , Condrocitos/metabolismo , Proteína Antagonista del Receptor de Interleucina 1/deficiencia , Interleucina-1beta/metabolismo , Cóndilo Mandibular/patología , Metaloproteinasa 13 de la Matriz/metabolismo , Ratones , Estrés Mecánico , Porcinos , Articulación Temporomandibular/patología
17.
Dent Med Probl ; 56(1): 81-87, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30951623

RESUMEN

BACKGROUND: Temporomandibular disorders (TMD) are the most common reason of non-dental pain in the orofacial region. A clinical examination of the temporomandibular joint (TMJ) with additional imaging is the most recommended procedure for TMD diagnosis. OBJECTIVES: The objective of this study was to evaluate the association between TMD and the condylar position in the glenoid fossa by examining a group of patients suffering from TMD compared with a control group of patients without TMD. In this study, we used cone-beam computed tomography (CBCT) images for measurements. MATERIAL AND METHODS: Sixty-five symptomatic joints were selected from 48 patients with TMD. Sixtyfive joints were selected from a total of 96 asymptomatic joints in the control group. The superior, anterior and posterior area of the joint, and the steepness of the articular eminence were measured on the CBCT images. The data was analyzed using Pearson's χ2 test. RESULTS: The position of the condyle was significantly more posterior in the joints with TMD, and more anterior and centric in the asymptomatic joints. Statistically, the vertical position of the condyle and the steepness of the articular eminence had no significant relation with the occurrence of TMD. CONCLUSIONS: In this study, we observed that the posterior condylar position is more common in TMD patients, but it is not the reason for diagnosing TMD, and the reason of the posterior position of the condyle should be investigated before any decisions pertaining to treatment are made. In future, studies should focus on evaluating how the position of the condyle will change after the treatment of patients with TMD.


Asunto(s)
Cóndilo Mandibular , Trastornos de la Articulación Temporomandibular , Tomografía Computarizada de Haz Cónico , Humanos , Cóndilo Mandibular/diagnóstico por imagen , Articulación Temporomandibular/diagnóstico por imagen , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/diagnóstico por imagen , Trastornos de la Articulación Temporomandibular/patología
18.
Diagn Cytopathol ; 47(8): 803-807, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-30908901

RESUMEN

Tophaceous pseudogout is a calcium pyrophosphate dihydrate crystal (CPPD) deposition disease that frequently affects elderly patient in the temporomandibular joint (TMJ). A diagnosis of CPPD deposition disease in the TMJ is challenging due to its mimicking of other benign and malignant entities. Surgical exploration followed by histologic examination is by far the most frequently used diagnostic modality. We present a case of an 87-year-old female who presented with a right TMJ mass. A final diagnosis of tophaceous pseudogout was made on cellular material obtained by ultrasound-guided fine-needle aspiration (US-guided FNA). Based on our case and current available literature, ultrasound-guided FNA is a reliable tool for diagnosing tophaceous pseudogout of the TMJ.


Asunto(s)
Condrocalcinosis/diagnóstico por imagen , Condrocalcinosis/patología , Articulación Temporomandibular/diagnóstico por imagen , Articulación Temporomandibular/patología , Ultrasonografía , Anciano de 80 o más Años , Biopsia con Aguja Fina , Femenino , Humanos , Biopsia Guiada por Imagen
19.
PLoS One ; 14(3): e0214072, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30897138

RESUMEN

End stage temporomandibular joint osteoarthritis (TMJ-OA) is characterized by fibrillations, fissures, clefts, and erosion of the mandibular condylar cartilage. The goal of this study was to define changes in pericellular and interterritorial delineations of the extracellular matrix (ECM) that occur preceding and concurrent with the development of this end stage degeneration in a murine surgical instability model. Two-photon fluorescence (TPF) and second harmonic generation (SHG) microscopy was used to evaluate TMJ-OA mediated changes in the ECM. We illustrate that TPF/SHG microscopy reconstructs the three-dimensional network of key fibrillar and micro-fibrillar collagens altered during the progression of TMJ-OA. This method not only generates spatially distinct pericellular and interterritorial delineations of the ECM but distinguishes early and end stage TMJ-OA by signal organization, orientation, and composition. Early stage TMJ-OA at 4- and 8-weeks post-injury is characterized by two structurally distinct regions containing dense, large fiber collagens and superficial, small fiber collagens rich in types I, III, and VI collagen oriented along the mesiodistal axis of the condyle. At 8-weeks post-injury, type VI collagen is locally diminished on the central and medial condyle, but the type I/III rich superficial layer is still present. Twelve- and 16-weeks post-injury mandibular cartilage is characteristic of end-stage disease, with hypocellularity and fibrillations, fissures, and clefts in the articular layer that propagate along the mediolateral axis of the MCC. We hypothesize that the localized depletion of interterritorial and pericellular type VI collagen may signify an early marker for the transition from early to end stage TMJ-OA, influence the injury response of the tissue, and underlie patterns of degeneration that follow attritional modes of failure.


Asunto(s)
Matriz Extracelular/patología , Osteoartritis/patología , Articulación Temporomandibular/patología , Animales , Colágeno/análisis , Masculino , Ratones , Microscopía de Fluorescencia por Excitación Multifotónica , Osteoartritis/diagnóstico por imagen , Microscopía de Generación del Segundo Armónico , Articulación Temporomandibular/diagnóstico por imagen , Articulación Temporomandibular/lesiones
20.
J Oral Sci ; 61(1): 82-88, 2019 Mar 28.
Artículo en Inglés | MEDLINE | ID: mdl-30814387

RESUMEN

This study evaluated the long-term effectiveness of intra-articular temporomandibular joint arthrocentesis for patients with osteoarthritis and compared arthrocentesis/lavage alone with arthrocentesis/lavage and injected hyaluronic acid. Forty patients met the inclusion criteria, and 37 completed long-term follow-up (approximately 4 years). The patients were randomly allocated to two groups: arthrocentesis with lavage alone (A-group, n = 17) or combined with hyaluronic acid treatment (AS-group, n = 20). Standard two-needle arthrocentesis was performed. Pain and joint sounds were measured at baseline and approximately 4 years after treatment. Reported pain, as indicated by visual analogue scale (VAS) score, significantly decreased from baseline to the final follow-up examination in both groups. Mean VAS score decreased from 64 to 16 (P < 0.001) in the A-group and from 63 to 25 (P < 0.001) in the AS-group. Average maximum incisor opening increased significantly in both groups but did not significantly differ between groups (P = 0.223). Joint sounds did not significantly improve within groups (A-group, P = 0.495; AS-group, P = 0.236). Both methods resulted in significant long-term improvements in pain and jaw function.


Asunto(s)
Artrocentesis/normas , Ácido Hialurónico/administración & dosificación , Osteoartritis/tratamiento farmacológico , Osteoartritis/cirugía , Articulación Temporomandibular/patología , Adulto , Femenino , Humanos , Ácido Hialurónico/uso terapéutico , Inyecciones Intraarticulares , Masculino , Persona de Mediana Edad
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