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1.
World J Microbiol Biotechnol ; 38(1): 18, 2022 Jan 03.
Artículo en Inglés | MEDLINE | ID: mdl-34977979

RESUMEN

Lantibiotics are a promising class of natural antimicrobial peptides. Lichenicidin is a two-peptide lantibiotic in which two mature peptides act synergistically to exhibit full bioactivity. Considering the two-peptide lantibiotics described so far, only cytolysin has been deeply characterized in terms of toxicity towards eukaryotic cells and it was found to be hemolytic and cytotoxic. This work aimed to improve the production of lichenicidin in vivo and characterize its antibacterial activity and toxicity against human cells. Peptides were purified and minimal inhibitory concentration (MIC) was determined against several strains; a time-kill assay was performed with Staphylococcus aureus. The hemolytic effect of lichenicidin was evaluated on blood samples from healthy donors and its toxicity towards human fibroblasts. The quantity of purified peptides was 1 mg/l Bliα and 0.4 mg/l Bliß. MIC for methicillin-sensitive and resistant S. aureus (MSSA and MRSA) strains were 16-32 µg/ml and 64-128 µg/ml, respectively. At the MIC, lichenicidin took less than 3 h to eliminate MSSA, indicating a strong bactericidal effect. It induces cell lysis at the highest concentration, an effect that might be potentiated by Bliß. Lichenicidin was not cytotoxic to human erythrocytes and fibroblasts. In this work, we evaluated the therapeutic potential of lichenicidin as a possible antimicrobial alternative.


Asunto(s)
Antiinfecciosos/farmacología , Bacterias/efectos de los fármacos , Infecciones Bacterianas/tratamiento farmacológico , Bacteriocinas/farmacología , Fibroblastos/efectos de los fármacos , Péptidos/farmacología , Secuencia de Aminoácidos , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Bacteriocinas/química , Bacteriocinas/aislamiento & purificación , Línea Celular , Relación Dosis-Respuesta a Droga , Sinergismo Farmacológico , Hemólisis , Humanos , Pruebas de Sensibilidad Microbiana
2.
Curr Microbiol ; 79(2): 64, 2022 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-35020062

RESUMEN

Secondary metabolites produced by microorganisms are the main source of antimicrobials and other pharmaceutical drugs. Soil microbes have been the primary discovery source for these secondary metabolites, often producing complex organic compounds with specific biological activities. Research suggests that secondary metabolism broadly shapes microbial ecological interactions, but little is known about the factors that shape the abundance, distribution, and diversity of biosynthetic gene clusters in the context of microbial communities. In this study, we investigate the role of nutrient availability on the abundance of biosynthetic gene clusters in soil-derived microbial consortia. Soil microbial consortia enriched in high sugar medium (150 mg/L of glucose and 200 mg/L of trehalose) had more biosynthetic gene clusters and higher inhibitory activity than those enriched in low sugar medium (15 mg/L of glucose + 20 mg/L of trehalose). Our results demonstrate that experimental microbial communities are a promising tool to study the ecology of specialized metabolites.


Asunto(s)
Microbiota , Suelo , Bacterias/genética , Nutrientes , Microbiología del Suelo
3.
J Environ Manage ; 304: 114321, 2022 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-35021593

RESUMEN

BACKGROUND: Mining activity in the Touissit district of Eastern Morocco has led to an unprecedented accumulation of heavy metals, mainly lead and zinc, in the tailing ponds of the open-air mines. This poses a real danger to both the environment and local population. OBJECTIVES: The goal of this work was to characterize the Plant Growth Promoting Rhizobacteria (PGPR) isolated from the rhizosphere soil of R. pseudoacacia plants grown wild in the abandoned Pb- and Zn-contaminated tailing ponds in the mining district of Touissit, in Eastern Morocco. MAIN RESULTS: One hundred bacterial strains were isolated from the rhizosphere of black locust (Robinia pseudoacacia L.) plants growing naturally in the Touissit mine tailings. Quantitative determination of indole-acetic and siderophores production, inorganic phosphate solubilization, hydrolysis of 1-aminocyclopropane-1-carboxylic acid (ACC deaminase activity), and ability to act as a biocontrol agent allowed selection of the 3 strains, 7MBT, 17MBT and 84MBT with improved PGP properties. The three strains grew well in the presence of high concentration of Pb-acetate and ZnCl2; and the addition of Pb or Zn to the culture medium differently affected the PGP properties analyzed. NOVELTY STATEMENT: Inoculation of black locust grown with the 3 selected strains, in the presence 1000 µg ml-1 of Pb-acetate, produced varying effects on the plant dry weight. The strain 84MBT alone or in combination with strains 7MBT and 17MBT increased significantly the dry weight of the plants by 91, 62, and 85% respectively. The 16S rRNA gene sequence analysis of each strain showed that the strains 7MBT 17MBT and 84MBT had 99.34, 100, and had 99.72% similarity with Priestia endophytica (formerly B. endophyticus), B. pumilus NBRC 12092T, and B. halotolerans NBRC 15718T, respectively.


Asunto(s)
Robinia , Contaminantes del Suelo , Bacterias/genética , Biodegradación Ambiental , Marruecos , ARN Ribosómico 16S/genética , Rizosfera , Suelo , Microbiología del Suelo , Contaminantes del Suelo/análisis
4.
Invest Ophthalmol Vis Sci ; 63(1): 5, 2022 01 03.
Artículo en Inglés | MEDLINE | ID: mdl-34985498

RESUMEN

Purpose: The gut microbiome has been linked to disease pathogenesis through their interaction in metabolic, endocrine, and immune functions. The goal of this study was to determine whether the gut and plasma microbiota could transfer microbes to the retina in type 1 diabetic mice with retinopathy. Methods: We analyzed the fecal, plasma, whole globe, and retina microbiome in Akita mice and compared with age-matched wild-type (WT) mice using 16S rRNA sequencing and metatranscriptomic analysis. To eliminate the contribution of the ocular surface and plasma microbiome, mice were perfused with sterile saline solution, the whole globes were extracted, and the neural retina was removed under sterile conditions for retinal microbiome. Results: Our microbiome analysis revealed that Akita mice demonstrated a distinct pattern of microbes within each source: feces, plasma, whole globes, and retina. WT mice and Akita mice experienced transient bacteremia in the plasma and retina. Bacteria were identified in the retina of the Akita mice, specifically Corynebacterium, Pseudomonas, Lactobacillus, Staphylococcus, Enterococcus, and Bacillus. Significantly increased levels of peptidoglycan (0.036 ± 0.001 vs. 0.023 ± 0.002; P < 0.002) and TLR2 (3.47 ± 0.15 vs. 1.99 ± 0.07; P < 0.0001) were observed in the retina of Akita mice compared to WT. Increased IBA+ cells in the retina, reduced a- and b-waves on electroretinography, and increased acellular capillary formation demonstrated the presence of retinopathy in the Akita cohort compared to WT mice. Conclusions: Together, our findings suggest that transient bacteremia exists in the plasma and retina of both cohorts. The bacteria found in Akita mice are distinct from WT mice and may contribute to development of retinal inflammation and barrier dysfunction in retinopathy.


Asunto(s)
Bacteriemia/microbiología , Bacterias/aislamiento & purificación , Retinopatía Diabética/microbiología , Heces/microbiología , Retina/microbiología , Animales , Bacterias/genética , Diabetes Mellitus Tipo 1/microbiología , Modelos Animales de Enfermedad , Electrorretinografía , Ensayo de Inmunoadsorción Enzimática , Ojo/microbiología , Microbioma Gastrointestinal/fisiología , Masculino , Ratones , Ratones Endogámicos C57BL , Microbiota/fisiología , ARN Ribosómico 16S/genética
5.
Arch Microbiol ; 204(2): 122, 2022 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-34994864

RESUMEN

Oxytetracycline (OTC), is a widely used veterinary antibiotic for treatment and prophylaxis in aquaculture. As an emerging pollutant, OTC in the environment exerts selective pressure on aquatic organisms causing proliferation of antibiotic resistant genes. In the present study, an OTC tolerant isolate labelled as pw2 was selected among the 11 OTC tolerant isolates, isolated from the aquaculture effluent, for investigating its OTC degrading potential. The cell morphology, biochemical characteristics, and 16S ribosomal RNA (rRNA) gene sequence of the isolated strain indicated that it belonged to the genus Planococcus. The OTC removal percentage was estimated through measuring its residual concentration in the culture medium with high performance liquid chromatography. The strain exhibited maximum removal efficiency of 90.62%, with initial OTC concentration of 10 µg/ml. The optimum degrading conditions were 35 °C and pH 7. The degradation rate of OTC with (biotic) and without strain pw2 (abiotic) was 3.253 and 1.149 mg/l/d, respectively. The half-life was recorded to be 2.13 d in the presence of strain pw2, in contrast to 6.03 days recorded without strain pw2. The total (biotic + abiotic) OTC degradation efficiency was 75.74, 83.93, 90.62, and 86.47% for the initial OTC concentrations of 1 to 25 µg/ml, respectively. Addition of carbon and nitrogen did not influence the OTC removal which indicates Planococcus sp. pw2 use OTC as sole energy source. Thus, Planococcus sp. pw2 plays a vital role in reducing the OTC concentration in the environment, offering a promising method for treatment of aquaculture effluent containing OTC.


Asunto(s)
Oxitetraciclina , Antibacterianos , Acuicultura , Bacterias , ARN Ribosómico 16S/genética
6.
Arch Microbiol ; 204(2): 123, 2022 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-34994917

RESUMEN

Several different techniques were employed for the isolation of Nannocystis from various sources. A polyphasic approach was used for identification. Twelve strains of N. pusilla, N. exedens, and N. konarekensis with distinctive distribution between climates were identified. The bioactivity was examined against a panel of eight bacteria, two yeasts, and one fungus; cytotoxicity was tested on the L929 fibroblast cell line. Eleven strains mainly inhibit Gram-positive bacteria, and only one isolate was cytotoxic. The extract analyses by HPLC and LC-MS were compared to Myxobase, and eight different compounds were detected; a correlation was observed between compounds and producing species. 70% of strains had the potential to produce structurally diverse compounds. Nannochelins and althiomycin were the most abundant metabolites. The discovery of a new species of Nannocystis and the high potentiality of strains to produce secondary metabolites encourage further sampling and in-depth analysis of extracts to find new active metabolites.


Asunto(s)
Antineoplásicos , Productos Biológicos , Myxococcales , Bacterias , Irán
7.
Zhonghua Jie He He Hu Xi Za Zhi ; 45(1): 78-82, 2022 Jan 12.
Artículo en Chino | MEDLINE | ID: mdl-35000310

RESUMEN

Respiratory tract infections (RTI) are a common and highly prevalent disease in the population, which can develop into acute respiratory distress syndrome (ARDS) in severe cases.A large variety of microorganisms can cause RTI, including bacteria, respiratory viruses, and fungi. The timely and accurate detection of these pathogens is the prerequisites of effective treatment of RTI. However, more than 50% of RTI patients failed to diagnosis of causative agents due to unavailability of qualified samples, antimicrobial treatment prior to sample collection, high variety of respiratory pathogens, and influence of the normal flora in respiratory tract. In recent years, progress on molecular diagnosis, especially the novel approaches such as clinical metagenomics and CRSIPR (Clustered regularly interspaced short palindromic repeats), has not only improved our capacity for RTI pathogen detection but also brought new challenges. In this review, we summed up the advances in RTI pathogen diagnosis in 2021 and discussed the clinical benefits and challenges from novel approaches, which provided a clinical perspective on the development and application of these diagnostic tools in the real world.


Asunto(s)
Trastornos Respiratorios , Infecciones del Sistema Respiratorio , Virus , Bacterias , Humanos , Sistema Respiratorio , Infecciones del Sistema Respiratorio/diagnóstico
8.
Clin Lab ; 68(1)2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-35023671

RESUMEN

BACKGROUND: Recently, microbiome of otitis media with effusion (OME) was investigated using high throughput sequencing (HTS) in children to discover unbiased causal bacteria and natural otitis media microbiomes. However, there are very few studies in the Asian population, and there are no studies in Koreans yet. METHODS: We investigated bacterial community of OME from 27 Korean children. Routine bacterial culture, PCR targeting six frequent bacteria, and 16S rRNA amplicon sequencing were performed on effusion samples. Medical records of patients were reviewed. RESULTS: The most common bacteria found in culture and PCR were coagulase negative Staphylococci and Hemophilus influenza, respectively. The most abundant taxon in 16S rRNA amplicon sequencing was Hemophilus. The bacteria that showed positive PCR were found to be the most abundant taxon in 16S rRNA amplicon sequencing. Alloiococcus was not found in all three methods. CONCLUSIONS: Our findings will contribute to a better understanding of causative agents of otitis media in children. The technical advancement of HTS in the clinical field will help further understanding.


Asunto(s)
Otitis Media con Derrame , Otitis Media , Bacterias/genética , Niño , Oído Medio , Humanos , Otitis Media con Derrame/diagnóstico , ARN Ribosómico 16S/genética
9.
Curr Microbiol ; 79(2): 60, 2022 Jan 04.
Artículo en Inglés | MEDLINE | ID: mdl-34982232

RESUMEN

The present study aims to carefully delineate the bacterial community composition in marine sediments from different geographical coastal regions of Palk Bay and Gulf of Mannar that are known for human recreational activities. Bacterial richness in different marine sediments was assessed using 16S rRNA gene-based Denaturing Gradient Gel Electrophoresis (DGGE) which is a widely deployed fingerprinting technique. The DGGE profiles revealed that the bacterial community profiles of sediment from different coastal regions were complex and dynamic. The most dominant phylum present in the marine sediment samples were Proteobacteria followed by Cyanobacteria, Bacteriodetes, Firmicutes, Acidobacteria, and Actinobacteria. Cosmopolitan presence of Thioalkalivibrio sp. was observed in all the marine sediments. Sequencing of the abundant band reveals the presence of Vibrio spp. in all the marine sediments. Comparative illumina data analysis revealed the presence of 51 different Vibrio species in which Vibrio alginolyticus holds the highest abundance (67.2%) followed by V. harveyi (13.5%). This is the one of the very few reports that compared the complex microbial community composition of the marine sediments of different geographical regions of unexplored coastal region. Further in-depth analysis needs to be taken to understand the presence of complex microbial compositions and their functions through high-throughput whole metagenome sequencing and metaproteomic approaches.


Asunto(s)
Bahías , Sedimentos Geológicos , Bacterias/genética , ADN Bacteriano/genética , ADN Ribosómico/genética , Humanos , Filogenia , ARN Ribosómico 16S/genética
10.
Curr Microbiol ; 79(2): 45, 2022 Jan 04.
Artículo en Inglés | MEDLINE | ID: mdl-34982248

RESUMEN

Caulobacter is a well-studied bacterial genus, but little is known about the plasmids that are found in some wild Caulobacter isolates. We used bioinformatic approaches to identify nine plasmids from seven different Caulobacter strains and grouped them based on their size and the similarity of their repABC, parAB, and mobAB genes. Protein pathway analysis of the genes on the K31p1 and K31p2 plasmids showed many metabolic pathways that would enhance the metabolic versatility of the host strain. In contrast, the CB4 plasmid contained 21 heavy metal resistance genes with the majority coding for proteins that enhance copper resistance. Growth assays of C. henricii CB4 demonstrated increased copper resistance and quantitative PCR showed an increase in the expression of eight heavy metal genes when induced with copper.


Asunto(s)
Caulobacter , Metales Pesados , Bacterias , Cobre , Plásmidos/genética
11.
J Agric Food Chem ; 70(2): 429-445, 2022 Jan 19.
Artículo en Inglés | MEDLINE | ID: mdl-34989570

RESUMEN

Quorum sensing (QS) is a signaling mechanism for cell-to-cell communication between bacteria, fungi, and even eukaryotic hosts such as plant and animal cells. Bacteria in real life do not exist as isolated organisms but are found in complex, dynamic, and microecological environments. The study of interspecies QS and interkingdom QS is a valuable approach for exploring bacteria-bacteria interactions and bacteria-host interaction mechanisms and has received considerable attention from researchers. The correct combination of QS signals and receptors is key to initiating the QS process. Compared with intraspecies QS, the signal regulation mechanism of interspecies QS and interkingdom QS is often more complicated, and the distribution of receptors is relatively wide. The present review focuses on the latest progress with respect to the distribution, structure, and signal transduction of interspecies and interkingdom QS receptors and provides a guide for the investigation of new QS receptors in the future.


Asunto(s)
Bacterias , Percepción de Quorum , Bacterias/genética , Plantas , Transducción de Señal
12.
Nutrients ; 14(1)2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-35011077

RESUMEN

Breastfeeding and introduction of solid food are the two major components of infant feeding practices that influence gut microbiota composition in early infancy. However, it is unclear whether additional factors influence the microbiota of infants either exclusively breastfed or not breastfed. We obtained 194 fecal samples from infants at 3-9 months of age, extracted DNA, and sequenced the V4 region of the 16S rRNA gene. Feeding practices and clinical information were collected by questionnaire and abstraction of birth certificates. The gut microbiota of infants who were exclusively breastfed displayed significantly lower Shannon diversity (p-adjust < 0.001) and different gut microbiota composition compared to infants who were not breastfed (p-value = 0.001). Among the exclusively breastfed infants, recipients of vitamin D supplements displayed significantly lower Shannon diversity (p-adjust = 0.007), and different gut microbiota composition structure than non-supplemented, breastfed infants (p-value = 0.02). MaAslin analysis identified microbial taxa that associated with breastfeeding and vitamin D supplementation. Breastfeeding and infant vitamin D supplement intake play an important role in shaping infant gut microbiota.


Asunto(s)
Lactancia Materna , Heces/microbiología , Microbiota/efectos de los fármacos , Vitamina D/administración & dosificación , Bacterias/clasificación , Índice de Masa Corporal , Suplementos Dietéticos , Femenino , Humanos , Lactante , Masculino
13.
PLoS One ; 17(1): e0262057, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35025938

RESUMEN

Respiratory tract infections (RTIs) are extremely common and can cause gastrointestinal tract symptoms and changes to the gut microbiota, yet these effects are poorly understood. We conducted a systematic review to evaluate the reported evidence of gut microbiome alterations in patients with a RTI compared to healthy controls (PROSPERO: CRD42019138853). We systematically searched Medline, Embase, Web of Science, Cochrane and the Clinical Trial Database for studies published between January 2015 and June 2021. Studies were eligible for inclusion if they were human cohorts describing the gut microbiome in patients with an RTI compared to healthy controls and the infection was caused by a viral or bacterial pathogen. Dual data screening and extraction with narrative synthesis was performed. We identified 1,593 articles and assessed 11 full texts for inclusion. Included studies (some nested) reported gut microbiome changes in the context of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) (n = 5), influenza (H1N1 and H7N9) (n = 2), Tuberculosis (TB) (n = 4), Community-Acquired Pneumonia CAP (n = 2) and recurrent RTIs (rRTI) (n = 1) infections. We found studies of patients with an RTI compared to controls reported a decrease in gut microbiome diversity (Shannon) of 1.45 units (95% CI, 0.15-2.50 [p, <0.0001]) and a lower abundance of taxa (p, 0.0086). Meta-analysis of the Shannon value showed considerable heterogeneity between studies (I2, 94.42). Unbiased analysis displayed as a funnel plot revealed a depletion of Lachnospiraceae, Ruminococcaceae and Ruminococcus and enrichment of Enterococcus. There was an important absence in the lack of cohort studies reporting gut microbiome changes and high heterogeneity between studies may be explained by variations in microbiome methods and confounder effects. Further human cohort studies are needed to understand RTI-induced gut microbiome changes to better understand interplay between microbes and respiratory health.


Asunto(s)
Microbioma Gastrointestinal/fisiología , Tracto Gastrointestinal/microbiología , Infecciones del Sistema Respiratorio/microbiología , Animales , Bacterias/crecimiento & desarrollo , Humanos
14.
Curr Microbiol ; 79(2): 58, 2022 Jan 04.
Artículo en Inglés | MEDLINE | ID: mdl-34982237

RESUMEN

The study aimed to evaluate the safety of copper ion sterilization based on copper ion residues in zebrafish (Brachydanio rerio), as well as bacterial community structure and diversity in recirculating aquaculture systems (RASs). The copper ion content was determined using national food safety standard GB 5009.13-2017. Bacterial community structures and alpha and beta diversity indexes were examined using the 16S rRNA gene sequences produced by Illumina HiSeq sequencing. The results revealed no significant copper ion enrichment in B. rerio when the copper ion concentration was 0.15 mg/L. The relative abundances of Erythrobacter, nitrite bacteria, and Flavanobacteria were clearly higher in the treatment group than in the control and differences in bacterial species richness and diversity were obvious. In addition, there was no sharp decrease in the microflora at the outflow of the copper ion generator. In conjunction with the changes in ammonia nitrogen, nitrate, and nitrite concentrations during the experiment, the results indicated that there were no significant effects on the purification efficacy of the biological filter, but the abundances of beneficial bacteria increased significantly. This is of great relevance in order to understand the response of bacterial communities affected by changing environmental conditions, such as copper ion sterilization.


Asunto(s)
Cobre , Pez Cebra , Animales , Acuicultura , Bacterias/genética , Cobre/farmacología , Agua Dulce , ARN Ribosómico 16S/genética , Esterilización
15.
World J Microbiol Biotechnol ; 38(2): 36, 2022 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-34993677

RESUMEN

4α-Glucanotransferase (4α-GTase) is unique in its ability to form cyclic oligosaccharides, some of which are of industrial importance. Generally, low amount of enzymes is produced by or isolated from their natural sources: animals, plants, and microorganisms. Heterologous expressions of these enzymes, in an attempt to increase their production for applicable uses, have been widely studied since 1980s; however, the expressions are mostly performed in the prokaryotic bacteria, mostly Escherichia coli. Site-directed mutagenesis has added more value to these expressed enzymes to display the desired properties beneficial for their applications. The search for further suitable properties for food application leads to an extended research in expression by another group of host organism, the generally-recognized as safe host including the Bacillus and the eukaryotic yeast systems. Herein, our review focuses on two types of 4α-GTase: the cyclodextrin glycosyltransferase and amylomaltase. The updated studies on the general structure and properties of the two enzymes with emphasis on heterologous expression, mutagenesis for property improvement, and their industrial applications are provided.


Asunto(s)
Sistema de la Enzima Desramificadora del Glucógeno/genética , Sistema de la Enzima Desramificadora del Glucógeno/metabolismo , Bacillus/enzimología , Bacillus/genética , Bacterias/enzimología , Escherichia coli/enzimología , Escherichia coli/genética , Escherichia coli/metabolismo , Glucosiltransferasas/genética , Glucosiltransferasas/metabolismo , Mutagénesis Sitio-Dirigida , Oligosacáridos , Levaduras/enzimología , Levaduras/genética , Levaduras/metabolismo
16.
World J Microbiol Biotechnol ; 38(3): 39, 2022 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-35018535

RESUMEN

The emergence of multidrug-resistant bacteria, viruses and tumors is a serious threat to public health. Among natural peptides, indolicidin, a 13-residue peptide belonging to the cathelicidin family, deserves special attention. Indolicidin has a broad spectrum of biological activity and is active against a wide range of targets, such as bacteria (Gram+ and Gram-), fungi and viruses. Here, we review the most important features of the biological activity, potential applications and perspectives of indolicidin and its analogs. Although not yet approved for commercialization, this peptide has great potential to be applied in different areas, including the medical, biomedical, food industry and other unexplored areas. To achieve this goal, a multidisciplinary team of researchers must work together to fine tune peptides that overall lead to novel analogs and formulations to combat existing and possibly future diseases.


Asunto(s)
Antibacterianos/farmacología , Péptidos Catiónicos Antimicrobianos/farmacología , Bacterias/efectos de los fármacos , Animales , Péptidos Catiónicos Antimicrobianos/genética , Biopelículas/efectos de los fármacos , Catelicidinas/genética , Catelicidinas/farmacología , Humanos , Pruebas de Sensibilidad Microbiana , Relación Estructura-Actividad
17.
BMC Bioinformatics ; 23(1): 22, 2022 Jan 06.
Artículo en Inglés | MEDLINE | ID: mdl-34991453

RESUMEN

BACKGROUND: As antibiotic resistance creates a significant global health threat, we need not only to accelerate the development of novel antibiotics but also to develop better treatment strategies using existing drugs to improve their efficacy and prevent the selection of further resistance. We require new tools to rationally design dosing regimens from data collected in early phases of antibiotic and dosing development. Mathematical models such as mechanistic pharmacodynamic drug-target binding explain mechanistic details of how the given drug concentration affects its targeted bacteria. However, there are no available tools in the literature that allow non-quantitative scientists to develop computational models to simulate antibiotic-target binding and its effects on bacteria. RESULTS: In this work, we have devised an extension of a mechanistic binding-kinetic model to incorporate clinical drug concentration data. Based on the extended model, we develop a novel and interactive web-based tool that allows non-quantitative scientists to create and visualize their own computational models of bacterial antibiotic target-binding based on their considered drugs and bacteria. We also demonstrate how Rifampicin affects bacterial populations of Tuberculosis bacteria using our vCOMBAT tool. CONCLUSIONS: The vCOMBAT online tool is publicly available at https://combat-bacteria.org/ .


Asunto(s)
Antibacterianos , Farmacorresistencia Bacteriana , Antibacterianos/farmacología , Bacterias/genética , Simulación por Computador , Modelos Biológicos
18.
Arch Microbiol ; 204(2): 138, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-35032195

RESUMEN

The numerous structural impacts on oil installations caused by corrosion make this issue a concern in the oil industry. Although chemical corrosion is relevant in this sector, it is indisputable that the microbial corrosion or bio-corrosion plays a preponderant role, with considerable economic losses. Microbial corrosion invariably depends on the formation of a biofilm on the attacked surface. Biofilm structures provide the conditions that favor the development of microbial groups related to corrosion. Despite the several microbial species are described as corrosive, certain groups, such as sulfate- and nitrate-reducing bacteria, acetogenic bacteria, and methanogenic archaea are the most commonly related. In spite of environmental factors influence the prevalence of certain species, it is increasingly accepted that the relationships between different species are determinant in corrosion. Such relationships can be evidenced by several surveys of microbial communities involved in bio-corrosion. Here, the main microbes related to corrosion in metallic structures used in oil installations are presented, as well as their metabolisms involved in the deterioration of metallic surfaces.


Asunto(s)
Archaea , Microbiota , Bacterias , Biopelículas , Corrosión
19.
Anal Chim Acta ; 1191: 339280, 2022 Jan 25.
Artículo en Inglés | MEDLINE | ID: mdl-35033245

RESUMEN

PGP-1 is a bacterial hydrolase that can hydrolyze the amide bond of the l-pyroglutamate (L-pGlu) residue at the amino terminus of proteins and peptides. Guided by the biological function of PGP-1, an off-on NIR fluorescent probe DDPA was developed for the visual sensing of PGP-1 by conjugating pyroglutamic acid (recognition group) and DDAN (fluorophore). Using intestinal bacteria cultivation, eight bacteria strains with active PGP-1 were identified and cultivated efficiently using DDPA. In addition, three natural inhibitors against PGP-1 were isolated from the medical herb Psoralea corylifolia, which could be used to interfere with bacterial metabolism in the gut. As such, the fluorescent probe DDPA provides an efficient method and potential tool for the investigation of intestinal microbiota.


Asunto(s)
Colorantes Fluorescentes , Microbioma Gastrointestinal , Bacterias
20.
ACS Appl Mater Interfaces ; 14(1): 49-56, 2022 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-34978405

RESUMEN

The development of low-cost, non-toxic, scalable antimicrobial textiles is needed to address the spread of deadly pathogens. Here, we report a polysiloxane textile coating that possesses two modes of antimicrobial inactivation, passive contact inactivation through amine/imine functionalities and active photodynamic inactivation through the generation of reactive oxygen species (ROS). This material can be coated and cross-linked onto natural and synthetic textiles through a simple soak procedure, followed by UV cure to afford materials exhibiting no aqueous leaching and only minimal leaching in organic solvents. This coating minimally impacts the mechanical properties of the fabric while also imparting hydrophobicity. Passive inactivation of Escherichia coli (E. coli) and methicillin-resistant Staphylococcus aureus (MRSA) is achieved with >98% inactivation after 24 h, with a 23× and 3× inactivation rate increase against E. coli and MRSA, respectively, when green light is used to generate ROS. Up to 90% decrease in the infectivity of SARS-CoV-2 after 2 h of irradiated incubation with the material is demonstrated. These results show that modifying textiles with dual-functional polymers results in robust and highly antimicrobial materials that are expected to find widespread use in combating the spread of deadly pathogens.


Asunto(s)
Antiinfecciosos/farmacología , Bacterias/efectos de los fármacos , Materiales Biocompatibles Revestidos/química , Polímeros/química , SARS-CoV-2/efectos de los fármacos , Textiles/análisis , Antiinfecciosos/química , COVID-19/prevención & control , COVID-19/virología , Materiales Biocompatibles Revestidos/farmacología , Escherichia coli/efectos de los fármacos , Humanos , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Fotoquimioterapia/métodos , Especies Reactivas de Oxígeno/metabolismo , SARS-CoV-2/aislamiento & purificación , Textiles/toxicidad , Rayos Ultravioleta
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