Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 840.906
Filtrar
2.
Zhonghua Yu Fang Yi Xue Za Zhi ; 54(3): 301-305, 2020 Mar 06.
Artículo en Chino | MEDLINE | ID: mdl-32187936

RESUMEN

Objective: To analyze the genomic recombination of the vibrio cholerae serogroup O1 El Tor pandemic strains. Methods: A total of 292 complete or draft genome sequences of Vibrio cholerae O1 serogroup El Tor strains isolated from 1937 to 2015 were selected from National Biotechnology Information Center database. The genome alignment of strains was computed by snippy software by using N16961 as reference sequence. Then ClonalFrameML software was used to do the recombinant analysis. The wilcox.test function in agricolae package was used to compare the number recombinant segments and the total length of recombinant regions between small and large chromosomes. The kruskal function was used to compare the number recombinant segments and the total length of recombinant regions among different isolation continents. The KOBAS tool was used to do the gene ontology enrichment analysis of recombinant hotspot genes. Results: Of all 292 strains of Vibrio cholerae, 163 strains (55.8%) were recombined. The median of normalized recombinant segment number of small chromosome was 4.7×10(-6) (9.3×10(-7), 2.0×10(-5)), which was significantly larger than that of large chromosome [2.4×10(-6) (3.4×10(-7), 5.7×10(-6))] (P<0.001). The median (P(25),P(75)) of recombinant segment number of strains isolated from Africa, Asia, Europe, North America and South America were 23(1.0,33.0), 1.0(0.0,34.0), 6.0(2.0,13.0), 0.0(0.0,1.0) and 29.5(6.8,56.8), respectively, and the difference was statistically significant (P<0.001). The median (P(25),P(75)) of total length of recombinant regions of strains isolated from Africa, Asia, Europe, North America and South America were 233.0(4.0, 461.0), 11.0(0.0, 695.5), 56.0(4.0,111.0), 0.0(0.0,9.0) and 347.5(132.8,1 323.5) bp, respectively, and the difference was statistically significant (P<0.001). Gene ontology Enrichment analysis showed that the functions of 62 recombinant hotspot genes were mainly enrichment in chemotaxis, taxis, response to external stimulus, receptor activity and molecular transducer activity. Conclustion: In this study, we found that there were significant differences in the number of recombinant fragments and the length of recombinant regions between large and small chromosomes of Vibrio cholerae El Tor. We also found significant differences in the number of recombinant fragments and the total length of recombinant regions among different continents.


Asunto(s)
Cólera/epidemiología , Genoma Viral , Pandemias , Recombinación Genética , Vibrio cholerae O1/genética , Cólera/diagnóstico , Cólera/microbiología , Variación Genética , Genómica , Humanos , Análisis de Secuencia de ADN , Vibrio cholerae O1/clasificación , Vibrio cholerae O1/virología
3.
Zhonghua Yu Fang Yi Xue Za Zhi ; 54(3): 323-326, 2020 Mar 06.
Artículo en Chino | MEDLINE | ID: mdl-32187940

RESUMEN

To conduct outbreak identification and transmission factor analysis of typhoid epidemic occurred in Xinqiao town, Jiangyin city from June to September 2016. A total of 14 strains of Salmonella typhi isolated from confirmed cases were collected, and 65 external environment samples and 13 food samples related to the outbreak were taken. Real-time PCR was used to detect specific gene of Salmonella typhi in the samples. Conventional method was used to isolate strains. The strains isolated from both the samples and patients in the epidemic were subjected to antimicrobial susceptibility testing and PFGE molecular characteristics. Salmonella typhi strain was isolated from one external sample (well water of a deli processing plant). The results of drug susceptibility showed that 15 strains were resistant to nalidixic acid. A total of 15 strains of Salmonella typhi were divided into 2 molecular patterns by pulsed field gel electrophoresis. The fingerprints of PFGE from the 13 patients and the environmental isolate were completely consistent, and there was one band difference from the other patient isolate. Combined with the epidemiological investigation and laboratory test results, it was determined that the outbreak was caused by genetic clone of the same Salmonella typhi. Food processing plant should be one of the key links.


Asunto(s)
ADN Bacteriano/genética , Brotes de Enfermedades , Epidemias , Salmonella enterica/genética , Salmonella typhi/genética , Fiebre Tifoidea/epidemiología , Antibacterianos/uso terapéutico , China/epidemiología , Farmacorresistencia Microbiana , Electroforesis en Gel de Campo Pulsado , Humanos , Pruebas de Sensibilidad Microbiana , Reacción en Cadena en Tiempo Real de la Polimerasa , Salmonella enterica/efectos de los fármacos , Salmonella enterica/aislamiento & purificación , Salmonella typhi/clasificación , Salmonella typhi/efectos de los fármacos , Salmonella typhi/aislamiento & purificación , Fiebre Tifoidea/diagnóstico , Fiebre Tifoidea/tratamiento farmacológico , Fiebre Tifoidea/microbiología
4.
Zhonghua Yu Fang Yi Xue Za Zhi ; 54(3): 327-331, 2020 Mar 06.
Artículo en Chino | MEDLINE | ID: mdl-32187941

RESUMEN

In recent years, the high infection rate of Helicobacter pylori and its impact on cardiovascular disease have attracted public attention. It may directly affect coronary heart disease, stroke, etc. through various mechanisms such as inflammation, immune response, and damage to endothelial cells. It could also play an important role in the formation of cardiovascular disease risk factors such as atherosclerosis, hypertension, hyperhomocysteinemia, and dyslipidemia. However, domestic and international research results are still inconsistent, and a large number of experiments are still required to confirm it to take effective measures to control the incidence of cardiovascular disease. This article reviews the prevalence of Helicobacter pylori and cardiovascular disease, the interaction mechanisms and the status of relevant domestic and international researches.


Asunto(s)
Enfermedades Cardiovasculares/microbiología , Infecciones por Helicobacter/complicaciones , Helicobacter pylori/aislamiento & purificación , Enfermedades Cardiovasculares/complicaciones , Células Endoteliales , Infecciones por Helicobacter/diagnóstico , Infecciones por Helicobacter/microbiología , Humanos , Factores de Riesgo
5.
Environ Monit Assess ; 192(4): 241, 2020 Mar 18.
Artículo en Inglés | MEDLINE | ID: mdl-32189082

RESUMEN

The present study aimed to assess and monitor the therapeutic potential of antimicrobial metabolites from marine sponge-associated bacteria collected from the southeast coast of India against multidrug-resistant clinical bacterial isolates. Five sponge samples were collected and the metabolite-producing bacteria were screened from the Gulf of Mannar, India, and their antibacterial potential was studied against drug-resistant clinical bacterial isolates obtained from the hospitals. The two metabolite-producing bacteria (IS1 and IS2) were characterized by standard microbiology protocols and 16S rRNA sequencing. The antibacterial metabolites were characterized by liquid chromatography mass spectrometry (LCMS) analysis. The study suggested that marine sponges such as Spheciospongia spp., Haliclona spp., Mycale spp., Tedania spp., and SS-01 were associated with 30 ± 2, 26 ± 2, 23 ± 3, 21 ± 2, and 20 ± 2% of antibacterial metabolite-producing bacteria, respectively. The LCMS analysis of metabolites extracted from IS1 (4,6-dimethyl-2-pyrimidinamine; 4,5-dimethyl-2-propylsilyl-1H-imidazole) and IS2 (caproyl amide, 2-imidazoline) associated with Spheciospongia spp. exhibited significant antibacterial properties against drug-resistant bacteria. IS1 showed antimicrobial potential against the clinical isolates of Proteus spp., and IS2 showed antibacterial potential against isolates of both Proteus mirabilis and Salmonella typhi. IS1 and IS2 were identified by 16S rRNA sequencing and designated as Klebsiella spp. DSCE-bt01 and Pseudomonas spp. DSCE-bt02, respectively. The current study concluded that the assessment and monitoring of novel isolates from sponge-associated bacteria from marine coastal areas probably offer latest breakthrough in curtailing the global antimicrobial resistance and the study of such ecosystems adds value addition to the searching of novel bioactive compounds from terrestrial ecosystems.


Asunto(s)
Bacterias , Ecosistema , Monitoreo del Ambiente , Poríferos , ARN Ribosómico 16S , Animales , Antibacterianos , Monitoreo del Ambiente/métodos , India , Pruebas de Sensibilidad Microbiana , Filogenia , Poríferos/microbiología
6.
J Insect Sci ; 20(2)2020 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-32112650

RESUMEN

Nine strains of five species of entomopathogenic hypocrealean fungi were tested against adults of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky). These strains have been developed as commercial biopesticide products in the United States, Brazil, South Korea, or the European Union (EU). Metarhizium anisopliae (Metschnikoff) (Hypocreales: Clavicipitaceae) ESALQ E-9 and Metarhizium brunneum (Petch) F52 (formerly M. anisopliae F52) (Hypocreales: Clavicipitaceae) killed 100% of treated beetles with the shortest survival times. Virulence differed among the five strains of Beauveria bassiana (Balsamo) (Hypocreales: Cordycipitaceae) tested, ranging from 0 to 77.3% mortality within 28 d. Two Isaria fumosorosea (Wize, 1904) (Hypocreales: Cordycipitaceae) (formerly Paecilomyces fumosoroseus) strains and the Lecanicillium muscarium (Petch) Zare & Gams (Hypocreales: Cordycipitaceae) strain used in Mycotal were not pathogenic to A. glabripennis adults. Within the entomopathogenic fungi tested, the Metarhizium strains may be the most appropriate for further evaluation.


Asunto(s)
Beauveria/patogenicidad , Escarabajos/microbiología , Metarhizium/patogenicidad , Control Biológico de Vectores/métodos , Animales , Hypocreales/patogenicidad , Virulencia
7.
World J Microbiol Biotechnol ; 36(3): 47, 2020 Mar 06.
Artículo en Inglés | MEDLINE | ID: mdl-32144505

RESUMEN

Beauveria bassiana (Bals.) Vuillemin (B. bassiana) is an entomopathogenic fungus that establishes endophytic symbiosis with plants. In the present study, the effects of B. bassiana strains colonization in growing Zea mays L. (Z. mays), crop production, and drought tolerance were evaluated. Z. mays seeds were inoculated with B. bassiana strains (GHA, PTG4, and PTG6), using 1 × 106 blastospores/mL and methyl cellulose (MC) or cornstarch (CS) as adherents. Colonization was determined by B. bassiana recovery from plant tissues plated on PDA medium. Plant height, fresh and dry weight, and flowering time were analyzed to assess plant performance. Drought tolerance was evaluated by stopping watering for 10 days, watering again, and determining vigor recovery after 24 h. Results showed 100% endophytic roots colonization, regardless of adherent type or strain tested. Colonization was variable in shoots and leaves, but GHA strain achieved the highest inoculation rates, including 88% in stems and 50% in leaves, which did not depend on adherent type used; for PTG4 strain, adherent type had an important effect (MC = 100% stems and leaves; CS = 63% stems and 25% leaves). For PTG6 strain, the best adherent type was CS (71% stems and 75% leaves), whereas MC showed variable inoculation percentage (25% stems and 75% leaves). Interestingly, only MCPTG4 treatment showed consistent positive effects on germination percentage (day 5 = 46 ± 2%; day 14 = 87 ± 7%) compared with controls (CC = 63 ± 4%, CMC = 50 ± 3%, CCS = 47 ± 0%). In addition, the other treatments showed low germination percentages at day 5 (7 ± 7% to 46 ± 2%), which recovered at day 14 (53 ± 0% to 73 ± 8%), except for MCPTG6 treatment with 23 ± 10% germination. About plant performance, not significant effects on plant height and fresh/dry weight in all the treatments were observed. However, B. bassiana-treated plants, using either GHA, PTG4 or PTG6 strains, and MC as adherent, showed tolerance to drought and flowered one to two weeks earlier, providing evidence supporting further applications of these seed treatments in agricultural systems, for abiotic stress sustainable management practices.


Asunto(s)
Beauveria/fisiología , Sequías , Endófitos/fisiología , Flores/crecimiento & desarrollo , Estrés Fisiológico , Zea mays/crecimiento & desarrollo , Germinación , Desarrollo de la Planta , Hojas de la Planta/microbiología , Semillas/crecimiento & desarrollo , Semillas/microbiología , Simbiosis , Zea mays/microbiología
8.
World J Microbiol Biotechnol ; 36(3): 51, 2020 Mar 10.
Artículo en Inglés | MEDLINE | ID: mdl-32157408

RESUMEN

Culture-independent molecular-based approaches can be used to identify genes of interest from environmental sources that have desirable properties such as thermo activity. For this study, a putative thermo stable endoglucanase gene was identified from a mixed culture resulting from the inoculation of Brock-CMcellulose (1%) broth with mudspring water from Mt. Makiling, Laguna, Philippines that had been incubated at 90 °C. Genomic DNA was extracted from the cellulose-enriched mixed culture and endo1949 forward and reverse primers were used to amplify the endoglucanase gene, which was cloned into pCR-script plasmid vector. Blastn alignment of the sequenced insert revealed 99.69% similarity to the glycosyl hydrolase, sso1354 (CelA1; Q97YG7) from Saccharolobus solfataricus. The endoglucanase gene (GenBank accession number MK984682) was determined to be 1,021 nucleotide bases in length, corresponding to 333 amino acids with a molecular mass of ~ 37 kDa. The endoglucanase gene was inserted into a pET21 vector and transformed in E. coli BL21 for expression. Partially purified recombinant Mt. Makiling endoglucanase (MM-Engl) showed a specific activity of 187.61 U/mg and demonstrated heat stability up to 80 °C. The thermo-acid stable endoglucanase can be used in a supplementary hydrolysis step to further hydrolyze the lignocellulosic materials that were previously treated under high temperature-dilute acid conditions, thereby enhancing the release of more glucose sugars for bioethanol production.


Asunto(s)
Celulasa/genética , Celulasa/metabolismo , Celulosa/metabolismo , ADN , Genómica , Agua/metabolismo , Secuencia de Aminoácidos , Archaea/enzimología , Archaea/genética , Bacterias/enzimología , Bacterias/genética , Secuencia de Bases , Clonación Molecular , Estabilidad de Enzimas , Escherichia coli/genética , Concentración de Iones de Hidrógeno , Peso Molecular , Filipinas , Proteínas Recombinantes , Alineación de Secuencia , Sulfolobales/enzimología , Sulfolobales/genética , Temperatura Ambiental , Microbiología del Agua
9.
Biomed Khim ; 66(1): 54-63, 2020 Jan.
Artículo en Ruso | MEDLINE | ID: mdl-32116226

RESUMEN

Numerous studies confirm the high degree of involvement of the intestinal microbiota in most processes in the human body. There is evidence for the effect of intestinal microbiota on the success of chemo and immunotherapy of oncological diseases. It is assumed that the intestinal microbiota exhibits an indirect effect on the antitumor therapy through such mechanisms as general immunomodulation, an increase in cells that specifically respond to antigens of both microbial and tumor origin, metabolism, degradation (utilization) of drug compounds. The intestinal microbiota is currently considered as an additional, but important target for studying the effective use of antitumor therapy and reducing its toxicity, as well as a predictor of the success of immunotherapy. In this review, we highlight the results of studies published to date that confirm the relationship between gut microbiome and antitumor efficacy of immune checkpoint inhibitors. Despite the promising and theoretically substantiated conclusions, there are still some discrepancies among the existing data that will have to be addressed in order to facilitate the further development of this direction.


Asunto(s)
Microbioma Gastrointestinal , Inmunoterapia , Intestinos/microbiología , Neoplasias/terapia , Humanos
10.
Medicine (Baltimore) ; 99(10): e19185, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32150056

RESUMEN

To investigate the characteristics of spiral computed tomography (CT), positron emission tomography-computed tomography (PET/CT) and clinical manifestations of talaromycosis to improve the diagnostic level and deepen its recognition in radiology.Radiological, clinical, and pathological manifestations of 15 patients of non-HIV talaromycosis confirmed by bronchofiberscope lung biopsy and/or abscess puncture fluid culture and/or blood culture and/or sputum culture were analyzed retrospectively. All patients underwent chest CT, among them, six had a brain MRI, and six had a PET/CT scan before treatment.On plain CT scan, there were multiple patches and massive consolidation in 6 patients, multiple patchy consolidations and patchy ground-glass opacities in 3 patients, solitary or multiple nodules and masses in 3 patients, multiple cavities and small nodules in 3 patients. Multiple lymphadenectasis appeared in bilateral hila, mediastinum, and neck in 10 patients. In contrast CT scan, the parenchyma of the lesions had a slight enhancement in 10 patients, moderate enhancement in 3 patients, obvious enhancement in 2 patients. Seven cases had bone destruction and hyperplasia, cranial involvement in 1 patient and liver involvement in 3 patients, respectively. On PET/CT, five patients showed elevated standard uptake value (SUV).The radiological manifestations of non-HIV talaromycosis show multiple consolidations, ground-glass opacities, multiple nodules or masses in bilateral lungs, deep-seated enlarged lymph nodes and bone destruction in multiple systems. The final diagnosis should be based on the culture of talaromycosis.


Asunto(s)
Enfermedades Pulmonares Fúngicas/diagnóstico por imagen , Micosis/diagnóstico por imagen , Adolescente , Adulto , Anciano , Biopsia , Niño , Preescolar , Diagnóstico Diferencial , Diagnóstico Precoz , Femenino , Fluorodesoxiglucosa F18 , Infecciones por VIH , Humanos , Lactante , Enfermedades Pulmonares Fúngicas/microbiología , Enfermedades Pulmonares Fúngicas/patología , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Micosis/microbiología , Micosis/patología , Tomografía Computarizada por Tomografía de Emisión de Positrones , Radiofármacos , Estudios Retrospectivos , Tomografía Computarizada por Rayos X , Adulto Joven
11.
Adv Exp Med Biol ; 1204: 75-127, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32152944

RESUMEN

Collectins are collagen-containing C-type (calcium-dependent) lectins which are important pathogen pattern recognising innate immune molecules. Their primary structure is characterised by an N-terminal, triple-helical collagenous region made up of Gly-X-Y repeats, an a-helical coiled-coil trimerising neck region, and a C-terminal C-type lectin or carbohydrate recognition domain (CRD). Further oligomerisation of this primary structure can give rise to more complex and multimeric structures that can be seen under electron microscope. Collectins can be found in serum as well as in a range of tissues at the mucosal surfaces. Mannanbinding lectin can activate the complement system while other members of the collectin family are extremely versatile in recognising a diverse range of pathogens via their CRDs and bring about effector functions designed at the clearance of invading pathogens. These mechanisms include opsonisation, enhancement of phagocytosis, triggering superoxidative burst and nitric oxide production. Collectins can also potentiate the adaptive immune response via antigen presenting cells such as macrophages and dendritic cells through modulation of cytokines and chemokines, thus they can act as a link between innate and adaptive immunity. This chapter describes the structure-function relationships of collectins, their diverse functions, and their interaction with viruses, bacteria, fungi and parasites.


Asunto(s)
Colectinas/inmunología , Inmunidad Innata , Inmunidad Adaptativa , Animales , Bacterias/inmunología , Hongos/inmunología , Humanos , Parásitos/inmunología , Virus/inmunología
12.
Adv Exp Med Biol ; 1204: 129-140, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32152945

RESUMEN

C-type lectins (CTLs) are a family of carbohydrate-recognition domain (CRD)-containing proteins that bind to ligands in a calcium-dependent manner. CTLs act as important components of insect innate immune responses, such as pattern recognition, agglutination, encapsulation, melanization, phagocytosis and prophenoloxidase activation, as well as gut microbiome homeostasis maintenance, to defend against pathogens. Besides, some insect CTLs can facilitate pathogen infection and colonization. In this review, we describe the properties of insect CTLs and focus on explaining their role in viral, bacterial, parasitic and fungal infections.


Asunto(s)
Inmunidad Innata/inmunología , Insectos/inmunología , Insectos/microbiología , Lectinas Tipo C/inmunología , Aglutinación/inmunología , Animales , Insectos/química
13.
Nature ; 579(7798): 250-255, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32161389

RESUMEN

The lithified lower oceanic crust is one of Earth's last biological frontiers as it is difficult to access. It is challenging for microbiota that live in marine subsurface sediments or igneous basement to obtain sufficient carbon resources and energy to support growth1-3 or to meet basal power requirements4 during periods of resource scarcity. Here we show how limited and unpredictable sources of carbon and energy dictate survival strategies used by low-biomass microbial communities that live 10-750 m below the seafloor at Atlantis Bank, Indian Ocean, where Earth's lower crust is exposed at the seafloor. Assays of enzyme activities, lipid biomarkers, marker genes and microscopy indicate heterogeneously distributed and viable biomass with ultralow cell densities (fewer than 2,000 cells per cm3). Expression of genes involved in unexpected heterotrophic processes includes those with a role in the degradation of polyaromatic hydrocarbons, use of polyhydroxyalkanoates as carbon-storage molecules and recycling of amino acids to produce compounds that can participate in redox reactions and energy production. Our study provides insights into how microorganisms in the plutonic crust are able to survive within fractures or porous substrates by coupling sources of energy to organic and inorganic carbon resources that are probably delivered through the circulation of subseafloor fluids or seawater.


Asunto(s)
Organismos Acuáticos/genética , Organismos Acuáticos/metabolismo , Metabolismo Energético/genética , Sedimentos Geológicos/microbiología , Microbiota/genética , Océanos y Mares , Ciclo del Carbono/genética , Perfilación de la Expresión Génica
14.
Br J Nurs ; 29(5): S6-S13, 2020 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-32167817

RESUMEN

Hard-to-heal wounds are a major public health problem that incur high economic costs. A major source of morbidity, they can have an overwhelming impact on patients, caregivers and society. In contrast to acute wound healing, which follows an 'orderly and timely reparative process', the healing of hard-to-heal wounds is delayed because the usual biological progression is interrupted. This article discusses hard-to-heal wounds, the impact they have on patients and healthcare systems, and how biofilms and other factors affect the wound-healing process. Controlling and preventing infection is of utmost importance for normal wound healing. Rational use of anti-infectious agents is crucial and is particularly relevant in the context of rising healthcare costs. Knowledge of the complex relationship between hard-to-heal wounds, biofilm formation and wound healing is vital for efficient management of hard-to-heal wounds.


Asunto(s)
Antiinfecciosos Locales/uso terapéutico , Biopelículas , Cicatrización de Heridas/fisiología , Infección de Heridas , Biopelículas/efectos de los fármacos , Biopelículas/crecimiento & desarrollo , Humanos , Infección de Heridas/microbiología , Infección de Heridas/prevención & control , Infección de Heridas/terapia
15.
Bone Joint J ; 102-B(3): 329-335, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32114807

RESUMEN

AIMS: Biopsy of the periprosthetic tissue is an important diagnostic tool for prosthetic joint infection (PJI) as it enables the detection of the responsible microorganism with its sensitivity to antibiotics. We aimed to investigate how often the bacteria identified in the tissue analysis differed between samples obtained from preoperative biopsy and intraoperative revision surgery in cases of late PJI; and whether there was a therapeutic consequence. METHODS: A total of 508 patients who required revision surgery of total hip arthroplasty (THA) (n = 231) or total knee arthroplasty (TKA) (n = 277) because of component loosening underwent biopsy before revision surgery. The tissue samples collected at biopsy and during revision surgery were analyzed according to the criteria of the Musculoskeletal Infection Society (MSIS). RESULTS: In total, 178 (113 THA, 65 TKA) were classified as infected. The biopsy procedure had a sensitivity of 93.8%, a specificity of 97.3%, a positive predictive value (PPV) of 94.9%, a negative predictive value (NPV) of 96.7%, and an accuracy of 96.1%. Of the 178 infected patients, 26 showed a difference in the detected bacteria from the biopsy and the revision surgery (14.6%). This difference required a change to antibiotic therapy in only two cases (1.1%). CONCLUSION: Biopsy is a useful tool to diagnose PJI, but there may be a difference in the detected bacteria between the biopsy and revision surgery. However, this did not affect the choice of antibiotic therapy in most cases, rendering the clinical relevance of this phenomenon as low. Cite this article: Bone Joint J 2020;102-B(3):329-335.


Asunto(s)
Artroplastia de Reemplazo de Cadera/efectos adversos , Artroplastia de Reemplazo de Rodilla/efectos adversos , Biopsia/métodos , Diagnóstico Tardío , Infecciones Relacionadas con Prótesis/diagnóstico , Adulto , Anciano , Anciano de 80 o más Años , Bacterias/aislamiento & purificación , Femenino , Estudios de Seguimiento , Articulación de la Cadera/microbiología , Articulación de la Cadera/patología , Articulación de la Cadera/cirugía , Prótesis de Cadera/efectos adversos , Prótesis de Cadera/microbiología , Humanos , Articulación de la Rodilla/microbiología , Articulación de la Rodilla/patología , Articulación de la Rodilla/cirugía , Prótesis de la Rodilla/efectos adversos , Prótesis de la Rodilla/microbiología , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Infecciones Relacionadas con Prótesis/microbiología , Reproducibilidad de los Resultados
16.
Bone Joint J ; 102-B(3): 336-344, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32114816

RESUMEN

AIMS: In the absence of an identified organism, single-stage revision is contraindicated in prosthetic joint infection (PJI). However, no studies have examined the use of intra-articular antibiotics in combination with single-stage revision in these cases. In this study, we present the results of single-stage revision using intra-articular antibiotic infusion for treating culture-negative (CN) PJI. METHODS: A retrospective analysis between 2009 and 2016 included 51 patients with CN PJI who underwent single-stage revision using intra-articular antibiotic infusion; these were compared with 192 culture-positive (CP) patients. CN patients were treated according to a protocol including intravenous vancomycin and a direct intra-articular infusion of imipenem and vancomycin alternately used in the morning and afternoon. In the CP patients, pathogen-sensitive intravenous (IV) antibiotics were administered for a mean of 16 days (12 to 21), and for resistant cases, additional intra-articular antibiotics were used. The infection healing rate, Harris Hip Score (HHS), and Hospital for Special Surgery (HSS) knee score were compared between CN and CP groups. RESULTS: Of 51 CN patients, 46 (90.2%) required no additional medical treatment for recurrent infection at a mean of 53.2 months (24 to 72) of follow-up. Impaired kidney function occurred in two patients, and one patient had a local skin rash. No significant difference in the infection control rate was observed between CN and CP PJIs (90.2% (46/51) versus 94.3% (181/192); p = 0.297). The HHS of the CN group showed no substantial difference from that of CP cases (79 versus 81; p = 0.359). However, the CN group showed a mean HSS inferior to that of the CP group (76 versus 80; p = 0.027). CONCLUSION: Single-stage revision with direct intra-articular antibiotic infusion can be effective in treating CN PJI, and can achieve an infection control rate similar to that in CP patients. However, in view of systemic toxicity, local adverse reactions, and higher costs, additional strong evidence is needed to verify these treatment regimens. Cite this article: Bone Joint J 2020;102-B(3):336-344.


Asunto(s)
Artritis Infecciosa/tratamiento farmacológico , Artroplastia de Reemplazo de Cadera/efectos adversos , Artroplastia de Reemplazo de Rodilla/efectos adversos , Imipenem/administración & dosificación , Cuidados Posoperatorios/métodos , Infecciones Relacionadas con Prótesis/tratamiento farmacológico , Vancomicina/administración & dosificación , Antibacterianos/administración & dosificación , Bacterias/aislamiento & purificación , Relación Dosis-Respuesta a Droga , Quimioterapia Combinada , Femenino , Estudios de Seguimiento , Articulación de la Cadera , Humanos , Inyecciones Intraarticulares , Articulación de la Rodilla , Masculino , Persona de Mediana Edad , Reoperación , Estudios Retrospectivos , Factores de Tiempo , Resultado del Tratamiento
17.
Bioresour Technol ; 304: 123064, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32115346

RESUMEN

In this study, a novel psychrotrophic lignocelluloses degrading microbial consortium LTF-27 was successfully obtained from cold perennial forest soil by successive enrichment culture under facultative anaerobic static conditions. The microbial consortium showed efficient degradation of rice straw, which cellulose, hemicelluloses and lignin lost 71.7%, 65.6% and 12.5% of its weigh, respectively, in 20 days at 15 °C. The predominant liquid products were acetic acid and butyric acid during degrading lignocellulose in anaerobic digestion (AD) process inoculated with the LTF-27. The consortium mainly composed of Parabacteroides, Alcaligenes, Lysinibacillus, Sphingobacterium, and Clostridium, along with some unclassified uncultured bacteria, indicating powerful synergistic interaction in AD process. A multi-species lignocellulolytic enzyme system working cooperatingly on lignocelluolse degradation was revealed by proteomics analysis of cellulose bound fraction of the crude extracellular enzyme, which provides key theoretical base for further exploration and application of LTF-27.


Asunto(s)
Consorcios Microbianos , Oryza , Bacterias , Celulosa , Lignina , Temperatura Ambiental
19.
Emerg Microbes Infect ; 9(1): 535-538, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32122270

RESUMEN

Worldwide emergence of Salmonella enterica serovar Newport (S. Newport) infection in humans, in parallel with a significant increasing prevalence of antimicrobial resistance (AR), is a serious public health concern. However, the prevalence of S. Newport resistance in China remains largely unknown. A retrospective study of 287 S. Newport clinical isolates collected during 1997-2018 was undertaken for characterization of AR profiles using the micro-dilution assay. We found a recent emergence of colistin resistance in four Chinese clinical isolates, including mcr-1-positive isolates. Importantly, phylogenomic and microbiological investigations indicate multiple independent clonal transmission of colistin-resistant S. Newport isolates of different seafood origins. Our study highlights potential reservoirs for transmission of colistin resistance and suggests that the global food supply chain may facilitate this dissemination.


Asunto(s)
Colistina/farmacología , Infecciones por Salmonella/microbiología , Salmonella enterica/aislamiento & purificación , Farmacorresistencia Bacteriana , Humanos , Salmonella enterica/efectos de los fármacos
20.
Infect Dis Poverty ; 9(1): 26, 2020 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-32160927

RESUMEN

BACKGROUND: Yaws is a chronic relapsing disease caused by Treponema pallidum subspecies pertunue, which can result in severe disability and deformities. Children below the age of 15 years in resource-poor communities are the most affected. Several non-specific factors facilitate the continuous transmission and resurgence of the disease. Endemic communities in rural Ghana continue to report cases despite the roll out of several intervention strategies in the past years. The objective of this study was to determine the factors associated with cutaneous ulcers among children in two yaws-endemic districts in Ghana. METHODS: A community-based unmatched 1:2 case-control study was conducted among children between 1 and 15 years. Data on socio-demographic, environmental and behavioral factors were collected using a structured questionnaire. Active case search and confirmation was done using the Dual Path Platform (DPP) Syphilis Screen and Confirm test kit. Data were analyzed using STATA 15. Logistic regression was done to determine the exposures that were associated with yaws infection at 0.05 significant level. RESULTS: Sixty-two cases and 124 controls were recruited for the study. The adjusted multivariable logistic regression model showed that yaws infection was more likely among individuals who reside in overcrowded compound houses (aOR = 25.42, 95% CI: 6.15-105.09) and with poor handwashing habits (aOR = 6.46, 95% CI: 1.89-22.04). Male (aOR = 4.15, 95% CI: 1.29-13.36) and increasing age (aOR = 5.90, 95% CI: 1.97-17.67) were also associated with yaws infection. CONCLUSIONS: Poor personal hygiene, overcrowding and lack of access to improved sanitary facilities are the factors that facilitate the transmission of yaws in the Awutu Senya West and Upper West Akyem districts. Yaws was also more common among males and school-aged children. Improving living conditions, access to good sanitary facilities and encouraging good personal hygiene practices should be core features of eradication programs in endemic communities.


Asunto(s)
Población Rural , Úlcera Cutánea/microbiología , Treponema pallidum/aislamiento & purificación , Buba , Adolescente , Estudios de Casos y Controles , Niño , Preescolar , Aglomeración , Enfermedades Endémicas , Femenino , Ghana/epidemiología , Humanos , Higiene , Lactante , Masculino , Vigilancia de la Población , Úlcera Cutánea/diagnóstico , Úlcera Cutánea/epidemiología , Encuestas y Cuestionarios , Serodiagnóstico de la Sífilis , Buba/diagnóstico , Buba/epidemiología , Buba/prevención & control , Buba/transmisión
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA