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1.
Bioresour Technol ; 329: 124919, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33676353

RESUMEN

Links between synergy and microbial community characteristics in co-digestion of food waste (FW), cattle manure (CM) and corn straw (CS) were investigated. Mono-digestion of FW and CS were inhibited by organic acids. Co-digestion of FW with CM achieved greater synergistic rates (18.5% and 22.3%) than CM with CS (14.8% and 12.3%). Synergy resulted from coupling effects of improving nutrient balance, dilution of toxic compounds, higher buffering capacity, detoxification based on co-metabolism, which ultimately reflected in microbial community functions. Although co-digestion of FW with CS exhibited lowest synergistic rates (7.9% and 4.9%), detoxification based on co-metabolism of syntrophic communities of Syntrophomonadaceae with hydrogenotrophic methanogens accelerated system recovery. Digester with the greatest synergy (65% FW + 35% CM) maintained dominant growth of hydrogenotrophic methanogens (68.9%), highest methanogenic community diversity and relative abundance of Methanosarcina (14.6%), which sustained more diverse and switchable methanogenic pathways therefore ensured powerful methanogenic functions and vigorous methanogenic capability.


Asunto(s)
Microbiota , Eliminación de Residuos , Anaerobiosis , Animales , Biocombustibles , Reactores Biológicos , Bovinos , Digestión , Alimentos , Estiércol , Metano , Zea mays
2.
Bioresour Technol ; 329: 124892, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33676356

RESUMEN

Subcritical dimethyl ether, a green solvent, was used to extract lipids from microalgae. The effect of the water content on the process was firstly investigated. Secondly, microalgal samples were subjected to five cell disruptions, and the effects on raw lipid and fatty acid methyl ester, and its profile were evaluated. Among them, heating, microwave, and ultrasonic treatments greatly improved extraction. Mechanism analysis revealed the improvements by the three treatments were due to increased cell wall permeability rather than to complete cell disruption. After the extraction, microalgal cells with lipid being well-extracted were shriveled with extensive surface folds, indicating a loss of intracellular substances, but the cell structure was undamaged. As for dewatering performance, extraction process removed almost all of the free water but left bound water. Finally, the potential of the residues after lipid extraction to serve as solid fuel was evaluated by combustion characteristics and heating value calculation.


Asunto(s)
Microalgas , Biocombustibles , Biomasa , Lípidos , Éteres Metílicos , Agua
3.
J Environ Manage ; 286: 112272, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33677337

RESUMEN

The recycling of biogas residues resulting from the anaerobic digestion of organic waste on agricultural land is among the means to reduce chemical fertilizer use and combat climate change. This in sacco decomposition study investigates (1) the potential of the granulated biogas residue fraction to provide nutrients and enhance soil carbon sequestration when utilized as exogenous organic matter in grassland soils, and (2) the impact of different nitrogen fertilizers on the organic matter decomposition and nutrient release processes. The experiment was conducted in two permanent grasslands of the Greater Region over one management period using rooibos tea as a comparator material. The decomposition and chemical changes of the two materials after incubation in the soil were assessed by measuring the mass loss, total carbon and nitrogen status, and fibre composition in cellulose, hemicellulose and lignin. Overall, after the incubation period, granulated biogas residue maintained up to 68% of its total mass, organic matter and total carbon; increased its content in recalcitrant organic matter by up to 45% and released 45% of its total nitrogen. Granulated biogas residue demonstrated resilience and a higher response uniformity when exposed to different nitrogen fertilizers, as opposed to the comparator material of rooibos tea. However, the magnitude of fertilizer-type effect varied, with ammonium nitrate and the combinatorial treatment of raw biogas residue mixed with urea leading to the highest organic matter loss from the bags. Our findings suggest that granulated biogas residue is a biofertilizer with the potential to supply nutrients to soil biota over time, and promote carbon sequestration in grassland soils, and thereby advance agricultural sustainability while contributing to climate change mitigation.


Asunto(s)
Secuestro de Carbono , Suelo , Agricultura , Biocombustibles , Carbono , Fertilizantes/análisis , Pradera , Nitrógeno/análisis
4.
Bioresour Technol ; 329: 124899, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33677422

RESUMEN

Chain elongation produce medium chain carboxylates, which are important precursors to many pharmaceuticals, antimicrobials and biofuels. Results in the presented investigations show that the supply of nano zero-valent iron (NZVI) can enhance caproate production. The highest caproate concentration achieved amounted to 27.2 mmol/L when 5 g/L NZVI were added, which was about 100% higher than the control. The study also showed increase of ethanol oxidation and decrease of butyrate and butanol with NZVI addition. Mechanism study showed NZVI can stimulate caproate production by preventing pH to fall below 5.4 through displacement reaction. Electron balance analysis displayed that NZVI provides extra electron by promoting ethanol oxidation and its dissolution. H2 was the potential electron shuttle between NZVI and chain elongators; High throughput sequencing showed function of NZVI on reshaping of microbial communities, especially enriching Oscillibacter Marseille-P3260, a kind of chain elongator and Corynebacterium which possesses fatty acid biosynthesis and iron utilization.


Asunto(s)
Hierro , Contaminantes Químicos del Agua , Biocombustibles , Electrones , Concentración de Iones de Hidrógeno
5.
Bioresour Technol ; 329: 124908, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33690058

RESUMEN

The cyanobacterial phycobiliproteins (PBPs) are an important natural colorant for nutraceutical industries. Here, a multi-objective hybrid machine learning-based optimization approach was used for enhanced cell biomass and PBPs production simultaneously in Nostoc sp. CCC-403. A central composite design (CCD) was employed to design an experimental setup for four input parameters, including three BG-11 medium components and pH. We achieved a 61.76% increase in total PBPs production and an almost 90% increase in cell biomass by our prediction model. We also established a test genome-scale metabolic network (GSMN) for Nostoc sp. and identified potential metabolic fluxes contributing to PBPs enhanced production. This study highlights the advantage of the hybrid machine learning approach and GSMN to achieve optimization for more than one objective and serves as the foundation for future efforts to convert cyanobacteria as an economically viable source for biofuels and natural products.


Asunto(s)
Nostoc , Ficobiliproteínas , Biocombustibles , Biomasa , Aprendizaje Automático
6.
Bioresour Technol ; 329: 124898, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33691204

RESUMEN

A novel strategy involving sodium dodecylsulfate (SDS) (SDS assisted tris (2-hydroxyethyl) methyl- ammonium methyl sulphate ([TMA][MeSO4], ionic liquid) pretreatment of Saccharum spontaneum biomass (SSB) following its enzymatic saccharification, and conversion into ethanol-biofuel in a consolidated bioprocess (CBP) was developed. Ionic liquid stable enzyme preparation developed from Bacillus subtilis G2 was used for saccharification. Optimized pretreatment and saccharification variables enhanced the sugar yield (2.35-fold), which was fermented to ethanol content of 104.42 mg/g biomass with an efficiency of 35.73%. The pretreated biomass was examined for textural/ultrastructural alterations by scanning electron microscopy (SEM), 1H/13C nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), surface area measurements, water retention value, and cellulase adsorption isotherms. The combined [TMA][MeSO4] and SDS pretreatment disrupted the lignocellulosic microfibrils, and increased the porosity and surface area. The study provides new mechanistic insights on combined IL and surfactant pretreatment of biomass for its efficient conversion to biofuel.


Asunto(s)
Celulasa , Saccharum , Biocombustibles , Biomasa , Etanol , Hidrólisis , Lignina , Espectroscopía Infrarroja por Transformada de Fourier
7.
Bioresour Technol ; 329: 124913, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33711716

RESUMEN

This study investigated the tolerance, defensive response and methanogenic pathways of anaerobic granular slugde and anaerobic suspended sludge (AGS and ASS) exposed to different LCM concentrations. AGS presented a higher tolerance to LCM stress, accompanied with 20.8 ± 2.6% enhancement in methane production at 1000 mg/L LCM, which was likely attributed to the less cell deaths and extracellular polymeric substances (EPSs) protection. In the acidification stage, acetate accumulation was stimulated and the activity of acetate kinase was promoted by LCM. In the methanogenesis stage, propionate and butyrate utilization for methane production were impaired after LCM addition. LCM also improved the activity of pyruvate-ferredoxin oxidoreductase and strengthened the process of hydrogenotrophic methanogenesis, likely by accelerating interspecies electron transfer mediated by hydrogen. ErmB and ermF were the dominate LCM resistance genes in AGS under LCM pressure conferring the resistance mechanism of ribosomal protection.


Asunto(s)
Biocombustibles , Aguas del Alcantarillado , Anaerobiosis , Reactores Biológicos , Lincomicina , Metano
8.
Bioresour Technol ; 329: 124932, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33713901

RESUMEN

Electro-fermentation system (EFS) emerges its effectiveness on treating microalgae for biodiesel production, but much is unknown about biodegradation behaviors, biodiesel characteristics, and microbial community. Compared with conventional fermentation system (CFS), microbial electrolysis cell-based EFS (MEC-EFS) and microbial fuel cell-based EFS (MFC-EFS) were investigated for the performance while treating microalgae Nannochloropsis sp. Results indicated that MEC-EFS presented much higher first-order decomposition rate coefficients of carbohydrates and proteins (1.212/d and 0.951/d) than those of CFS (0.615/d and 0.794/d) and MFC-EFS (0.518/d and 0.415/d). Compared with MFC-EFS, MEC-EFS showed better electrochemical performance (2.17 A/m3vs. 0.95 A/m3). Moreover, MEC-EFS reached the highest extracted lipid to biomass ratio (43.3%), followed by MFC-EFS (32.3%) and CFS (27.7%). By strengthened microbial biohydrogenation, MEC-EFS and MFC-EFS had higher saturated fatty acids ratio (78.8% and 70.6%) than that of CFS (56.1%). For MEC-EFS, enriched Ruminococcus and Geobacter in anodic biofilm might contribute to favorable biohydrogenation and electrochemical performance.


Asunto(s)
Fuentes de Energía Bioeléctrica , Microalgas , Microbiota , Estramenopilos , Biocombustibles , Fermentación
9.
Bioresour Technol ; 329: 124935, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33713900

RESUMEN

Sargassum spp is an invasive macroalgae and an alternative feedstock for bioethanol production. Sargassum spp biomass was subjected to high-pressure technology for biomass fractionation under different operating conditions of temperature and residence time to obtain glucan enriched pretreated solids (32.22 g/100 g of raw material). Enzyme hydrolysis process at high pretreated solid loading (13%, w/v) and enzyme loading of 10 FPU/g of glucan was performed, obtaining 43.01 g/L of glucose corresponding to a conversion yield of 92.12%. Finally, a pre-simultaneous saccharification and fermentation strategy (PSSF) was performed to produce bioethanol. This operational strategy produced 45.66 g/L of glucose in the pre-saccharification stage, and 18.14 g/L of bioethanol was produced with a glucose to bioethanol conversion yield of 76.23%. The development of this process highlights the feasibility of bioethanol production from macroalgal biomass in the biorefinery concept.


Asunto(s)
Sargassum , Biocombustibles , Biomasa , Etanol , Fermentación , Hidrólisis , Tecnología
10.
Bioresour Technol ; 330: 124953, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33725519

RESUMEN

To enhance microalgal lipid production, canonical two-step cultivation strategy that by transferring the microalgal cells grown in nutrient-replete medium to nutrient-depleted medium is widely used. However, the harvesting step during the transfer raises the production cost. To avoid the harvesting step, this study developed a continuous two-step (CTS) cultivation strategy. In the strategy, Chlorella sacchrarophila was grown in bioreactor while a xylanolytic bacterium Cellvibrio pealriver grown in an inner bag that embedded in the bioreactor; after the first-step co-cultivation, the inner bag is removed which then start the second-step cultivation of C. sacchrarophila. Based on the strategy, the lipid production was determined as 825.34-929.79 mg·L-1, which were 1.7-1.9 times higher than that of cultivation in canonical two-step strategy using glucose as feedstock. During the CTS strategy, the co-cultivation using xylan as feedstock promotes the microalgal growth and the removal of inner bag produces nutrient-depleted condition for enhancing microalgal lipid production.


Asunto(s)
Chlorella , Microalgas , Biocombustibles , Biomasa , Lípidos
11.
Bioresour Technol ; 330: 124965, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33735725

RESUMEN

With the increasing of data in wastewater treatment, data-driven machine learning models are useful for modeling biological processes and complex reactions. However, few data-driven models have been developed for simulating the microbial electrolysis cells (MECs) and traditional models are too ambiguous to comprehend the mechanisms. In this study, a new general data-driven two-stage model was firstly developed to predict CH4 production from in-situ biogas upgrading in the biocathode MECs via direct electron transfer (DET), named NARX-BP hybrid neural networks. Compared with traditional one-stage model, the model could well predict methane production via DET with excellent performance (all R2 and MES of 0.918 and 6.52 × 10-2, respectively) and reveal the mechanisms of biogas upgrading, for the new systematical modeling approach could improve the versatility and applicability by inputting significant intermediate variables. In addition, the model is generally available to support long-term prediction and optimal operation for anaerobic digestion or complex MEC systems.


Asunto(s)
Biocombustibles , Metano , Anaerobiosis , Reactores Biológicos , Electrólisis , Electrones , Aprendizaje Automático , Redes Neurales de la Computación
12.
Bioresour Technol ; 330: 124971, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33740584

RESUMEN

This study investigates the suitability of continuous hybrid fixed bed anaerobic filter reactor for treating sewage and agro-industrial digestate hydrothermal carbonization (HTC) products; hydrochar and HTC liquor (HTCL). The reactor was operated for 300 days under mesophilic conditions at different organic loading rates (OLR); maximum OLRs of 7.4 and 10 gCOD/L/d were reached while treating HTC liquor and slurry, respectively. 15 g/L hydrochar were added to the reactor as a supplement while treating HTCL solely thus increasing the biogas production up to 153%. The reactor was fed with HTCL and hydrochar with an increasing mixing ratio, and the co-digestion impact was dependent on hydrochar concentrations. The results of the study indicate that the hybrid fixed bed anaerobic filter reactor is a promising anaerobic digestion configuration for treating HTCL and overcoming the HTC upscaling challenges, and the suitability of digestate hydrochar utilization as supplement material for anaerobic digestion.


Asunto(s)
Biocombustibles , Aguas del Alcantarillado , Anaerobiosis , Suplementos Dietéticos
13.
Bioresour Technol ; 330: 124962, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33740585

RESUMEN

This study aimed to investigate the effect of bioaugmentation on microbial community and function in a continuous anaerobic process treating lignocellulosic cow manure. One reactor (Rb) received bioaugmentation dosage for a certain period (d100-d170) and stopped afterward (d170-d220), while the same applied to the control (Rc) except sterilized bioaugmentation dosage was introduced. Samples were taken on day130, 170 and 220 from both reactors for metatranscriptomic analysis. The results underlined the promotive effect of bioaugmentation on indigenous microorganisms regarding hydrolysis and methanogenesis. Bioaugmentation contributed to the enrichment of Clostridium, Cellvibrio, Cellulomonas, Bacillus, Fibrobacter, resulting in enhanced cellulase activity (Rb: 0.917-1.081; Rc: 0.551-0.677). Moreover, bioaugmentation brought Rb the prosperity of uncultured_ Bathyarchaeia, a prominent archaeal group responsible for the improved methyl-coenzyme M reductase activity, thus accelerated methanogenesis. Unique metabolic pathways (autotrophic carbon fixation and methanogenesis) in uncultured_ Bathyarchaeia broadened the horizon of its fundamental role as acetogens and methanogens in anaerobic digestion.


Asunto(s)
Euryarchaeota , Estiércol , Anaerobiosis , Animales , Biocombustibles , Reactores Biológicos , Bovinos , Femenino , Metano
14.
Bioresour Technol ; 330: 124986, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33744738

RESUMEN

The aim of this work was to study an innovative alkaline process on two cover crops. CaO load of 60 g.kgTS-1 was implemented to combine the functions of storage and pretreatment. Lab-scale reactors were monitored for 180 days to assess the effect of this process on the physico-chemical properties of the biomass. From the first days, pH was not maintained in an alkaline zone and microbial fermentation activity was observed with the degradation of available carbohydrates and production of metabolites, CO2 and H2. High butyric acid accumulation was observed and mass losses of 18.1% and 9.0% of initial VS occurred for oat and rye, respectively. However, no methane potential loss was recorded in the short and long term and the crops were efficiently preserved. The pretreatment had no major impact on fiber solubilization, and no increase in BMP was obtained, which was attributed to the short duration of the alkaline conditions.


Asunto(s)
Productos Agrícolas , Metano , Anaerobiosis , Biocombustibles , Biomasa , Fermentación
15.
Bioresour Technol ; 330: 125001, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33773269

RESUMEN

Anaerobic digestion is a long-established technology for the valorization of diverse organic wastes with concomitant generation of valuable resources. However, mono-digestion (i.e., anaerobic digestion using one feedstock) suffers from challenges associated with feedstock characteristics. Co-digestion using multiple feedstocks provides the potential to overcome these limitations. Significant research and development efforts have highlighted several inherent merits of co-digestion, including enhanced digestibility due to synergistic effects of co-substrates, better process stability, and higher nutrient value of the produced co-digestate. However, studies focused on the underlying effects of diverse co-feedstocks on digester performance and stability have not been synthesized so far. This review fills this gap by highlighting the limitations of mono-digestion and critically examining the benefits of co-digestion. Furthermore, this review discusses synergistic effect of co-substrates, characterization of microbial communities, the prediction of biogas production via different kinetic models, and highlights future research directions for the development of a sustainable biorefinery.


Asunto(s)
Reactores Biológicos , Microbiota , Anaerobiosis , Biocombustibles , Digestión , Metano
16.
Bioresour Technol ; 330: 124999, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33780712

RESUMEN

The energy potential of residue-derived biogas via electricity and biomethane production was assessed in an integrated 1G2G sugarcane biorefinery concept. The mono-digestion of 1G-vinasse (1G-VN) was compared with different co-digestion systems, namely, 1G-VN + filter cake (FC) + deacetylation liquor (DL) in the season and FC + DL in the off-season. Gross energy output values and the resulting sugarcane use efficiency were also assessed in different biorefinery schemes. Electricity production from 1G to VN (5.0 MW) could be increased by over 400% through its co-digestion with FC and DL (22.3 MW). Alternatively, biomethane could fully supply the diesel-powered fleet (1.8 × 106 Nm3 month-1) of a sugarcane plant processing 10 million tons of sugarcane per harvest, and the surplus biogas could flexibly provide 36 MW of extra electricity. Biomethane could enhance the energy output of 1G2G sugarcane biorefineries by 15%. However, 2G processes still require marked improvements to maximize energy production from sugarcane.


Asunto(s)
Saccharum , Anaerobiosis , Biocombustibles , Digestión , Electricidad , Metano
17.
Huan Jing Ke Xue ; 42(4): 1979-1988, 2021 Apr 08.
Artículo en Chino | MEDLINE | ID: mdl-33742833

RESUMEN

In order to reveal the impact of the application of biogas slurry instead of chemical nitrogen fertilizer on the environmental risk of heavy metals in the soil by returning straw to the field, four treatments, without biogas slurry and without straw applications (CK), biogas slurry application without straw (B), straw application without biogas slurry (S), and biogas slurry combined with straw applications (BS), were applied in a typical coastal reclaimed farmland (rice-wheat rotation) in Jiangsu province. The migration and morphological characteristics of Cu, Zn, Cd, and Pb in different soil layers were observed, and the potential environmental risks were estimated. The results showed that:① The total amounts of Zn and Pb in the surface soils (0-20 cm) in the rice and wheat fields under the BS treatment decreased significantly (P<0.05). The four heavy metals in the paddy soils migrated 6%-11% from the surface to the middle and lower layers (20-60 cm), and Cu, Cd, and Pb in the wheat soils migrate down from the surface by 25% to 33%. This indicated that the combined use of biogas slurry and straw accelerates the vertical downward movement of heavy metals in the surface soil. ② Under the BS treatment, the contents of the weak acid extraction of Cu in the surface soil of the paddy field decreased by 8.8%, and the residual state of Zn, Cd, and Pb decreased by 7.0% to 14.2%. This revealed that Cu was passivated, but Zn, Cd, and Pb tended to be activated. In comparison, the reduction in Cu residues in wheat field surface soil was 2.8 times that of the weak acid extraction, indicating that Cu was activated. Furthermore, the residue state of Cd increased, the weak acid extraction state of Pb decreased, and Cd and Pb were passivated. ③ The ecological risk assessment of heavy metals showed that there is no ecological risk in the soils under the BS treatment, and the risk indices were significantly lower than those of the B and S treatments (P<0.05). Therefore, the combined use of biogas slurry and straw helps to significantly reduce the risk of heavy metal pollution in the soils in the coastal reclamation areas.


Asunto(s)
Metales Pesados , Oryza , Contaminantes del Suelo , Biocombustibles , China , Monitoreo del Ambiente , Metales Pesados/análisis , Rotación , Suelo , Contaminantes del Suelo/análisis , Triticum
18.
Water Res ; 196: 117038, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33751972

RESUMEN

It is necessary to develop sustainable technologies for centrate wastewater (CW) and biogas treatment from sludge anaerobic digestion (AD) systems in an environmentally friendly and economical manner. The microalgae-based bioremediation approach presents a competitive alternative due to its capacity for nutrient recovery and carbon sequestration. However, process instabilities and operating challenges limit its development and implementation largely due to the complexities in the CW and biogas. In this study, the evolved native microalgal consortium (ENMC) was firstly developed using the gradual stress increase method to enhance their adaptation in high ammonium condition. The supplementation of local snow (with Ca2+ and Mg2+) and biogas into CW significantly enhanced ENMC growth through batch tests. Subsequently, an integrated ENMC-snow (ENMCS) system was proposed consisting of a hydrolysis-acidification reactor (HAR), biogas upgrade reactor, and photobioreactor (PBR). The ENMCS system was systematically investigated under both batch and semi-continuous operations, by adjusting primary process parameters including the fill ratio, feeding time, hydraulic retention time (HRT), wastewater pretreatment, and PBR type. It was eventually optimized as a 24 h, 70% fermented CW diluted with 30% snow water, semi-continuous feeding system with a fill ratio of 50% and HRT of 6 d in an open-PBR. Long-term operation (310 days) showed superior biomass yield (0.3059 ± 0.0039 g/(L•d)) and nutrient removal efficiencies (95.6 ± 0.13% and 90.8 ± 0.44% for NH4+-N and PO43--P removal). Meanwhile, biogas was upgraded with an 82.2% CO2 reduction. The economic and environmental analysis further demonstrated the ENMCS system as an effective alternative for the bioremediation of AD effluents while simultaneously producing value-added biomass, especially applicable to snowy regions.


Asunto(s)
Microalgas , Biodegradación Ambiental , Biocombustibles/análisis , Biomasa , Nieve , Aguas Residuales
19.
Sheng Wu Gong Cheng Xue Bao ; 37(3): 806-815, 2021 Mar 25.
Artículo en Chino | MEDLINE | ID: mdl-33783151

RESUMEN

Yeast are comprised of diverse single-cell fungal species including budding yeast Saccharomyces cerevisiae and various nonconventional yeasts. Budding yeast is well known as an important industrial microorganism, which has been widely applied in various fields, such as biopharmaceutical and health industry, food, light industry and biofuels production. In the recent years, various yeast strains from different ecological environments have been isolated and characterized. Novel species have been continuously identified, and strains with diverse physiological characteristics such as stress resistance and production of bioactive compounds were selected, which proved abundant biodiversity of natural yeast resources. Genome mining of yeast strains, as well as multi-omics analyses (transcriptome, proteome and metabolome, etc.) can reveal diverse genetic diversity for strain engineering. The genetic resources including genes encoding various enzymes and regulatory proteins, promoters, and other elements, can be employed for development of robust strains. In addition to exploration of yeast natural diversity, phenotypes that are more suitable for industrial applications can be obtained by generation of a variety of genetic diversity through mutagenesis, laboratory adaptation, metabolic engineering, and synthetic biology design. The optimized genetic elements can be used to efficiently improve strain performance. Exploration of yeast biodiversity and genetic diversity can be employed to build efficient cell factories and produce biological enzymes, vaccines, various natural products as well as other valuable products. In this review, progress on yeast diversity is summarized, and the future prospects on efficient development and utilization of yeast biodiversity are proposed. The methods and schemes described in this review also provide a reference for exploration of diversity of other industrial microorganisms and development of efficient strains.


Asunto(s)
Ingeniería Metabólica , Saccharomyces cerevisiae , Biodiversidad , Biocombustibles , Microbiología Industrial , Saccharomyces cerevisiae/genética , Biología Sintética
20.
Sheng Wu Gong Cheng Xue Bao ; 37(3): 816-830, 2021 Mar 25.
Artículo en Chino | MEDLINE | ID: mdl-33783152

RESUMEN

Due to abundant availability of shale gas and biogas, methane has been considered as one of the most potential carbon sources for industrial biotechnology. Methanotrophs carrying the native methane monooxygenase are capable of using methane as a sole energy and carbon source, which provides a novel strategy for reducing greenhouse gas emission and substituting edible substrates used in bioconversion processes. With the rapid development of genetic engineering tools and biosynthesis techniques, various strategies for improving the efficiency of methane bioconversion have been achieved to produce a variety of commodity bio-based products. Herein, we summarize several important aspects related with methane utilization and metabolic engineering of methanotrophs, including the modification of methane oxidation pathways, the construction of efficient cell factories, and biosynthesis of chemicals and fuels. Finally, the prospects and challenges of the future development of methane bioconversion are also discussed.


Asunto(s)
Biocombustibles , Metano , Biotecnología , Ingeniería Metabólica , Oxidación-Reducción
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