Sublethal toxicity of carbofuran in cattle egret (Bubulcus ibis coromandus): hematological, biochemical, and histopathological alterations / Toxicidade subletal do carbofurano na garça-vaqueira (Bubulcus ibis coromandus): alterações hematológicas, bioquímicas e histopatológicas

Abstract This study was aimed to investigate Carbofuran (CF)-induced pathological changes in cattle egret. Two hundred cattle egrets were reared and equally divided into four groups and given different CF concentrations (0.03 mg/L, 0.02 mg/L, 0.01 mg/L and 0 mg/L (control group)). Hematology, serum biochemistry, histopathology, and immunological markers were studied. Our results confirm that CF induces anemic conditions, leukocytosis, elevated liver enzymatic activity, and alterations in renal biomarkers. Moreover, specific microscopic lesions such as multifocal necrosis, pyknotic nuclei, hemorrhages, congestion, and inflammatory cell proliferation were observed in the liver, kidney, spleen, and thymus. These findings suggest that CF can induce harmful effects, so the application of this pesticide in the field must be strictly monitored to mitigate the possibility of exposure to non-target species.

Resumo Este estudo teve como objetivo investigar as alterações patológicas induzidas por carbofurano (CF) em garças-vaqueiras. Duzentas dessas garças foram criadas e divididas igualmente em quatro grupos e receberam diferentes concentrações de CF: 0,03 mg/L; 0,02 mg/L; 0,01 mg/L; e 0 mg/L (grupo controle). Foram realizadas análises de hematologia, bioquímica sérica, histopatologia e marcadores imunológicos. Nossos resultados confirmaram que CF induz condições anêmicas, leucocitose, atividade enzimática hepática elevada e alterações nos biomarcadores renais. Além disso, lesões microscópicas específicas, como necrose multifocal, núcleos picnóticos, hemorragias, congestão e proliferação de células inflamatórias, foram observadas no fígado, rim, baço e timo. Esses achados sugerem que o CF pode causar efeitos nocivos, portanto a aplicação desse agrotóxico no campo deve ser rigorosamente monitorada para mitigar a possibilidade de exposição a espécies não alvo.

Effects of Pasturella Multocida B: 2 and its immunogens (LPS and OMP) on reproductive hormones in Nili-Ravi Buffaloes / Efeitos de Pasteurella multocida B: 2 e seus imunógenos (LPS e OMP) em hormônios reprodutivos em búfalos Nili-Ravi

Abstract Livestock is a fundamental part of the agriculture industry in Pakistan and contributes more than 11.53% to GDP. Among livestock species, the buffaloes are regarded as the black gold of Pakistan. Being the highest milk producers globally, Nili-Ravi buffaloes are the most famous ones. Buffaloes are affected by many endemic diseases, and "Hemorrhagic septicemia" (HS) is one of them. This study was designed to ascertain the effects of experimental exposure ofP. multocida B:2 (oral) and its immunogens, i.e., LPS (oral and intravenous) and OMP (oral and subcutaneous) on reproductive hormonal profiles in Nili-Ravi buffaloes. Repeated serum samples were collected from the jugular vein of experimental animals for 21 days (0, 02, 04, 08, 12, 16, 20, 24, 36, 48, 72, 120, 168, 216, 264, 360, 456 and 504 hours). Hormonal assays to determine the serum concentrations of Gonadotropin-releasing hormone (GnRH), Follicle-stimulating hormone (FSH), Luteinizing hormone (LH), Estrogen (E2) and progesterone (P4) were performed using (MyBioSource) commercial Elisa kits. The hormonal profile of all treatment groups of the buffalo heifers exhibited significant (P<0.05) variations as compared to the control group (G-1). These results indicate suppression in Nili-Ravi buffaloes' reproductive hormonal profile on exposure to P. multocida B:2 and its immunogens. This influence warrants that exposure to H.S may be a possible reason for delayed puberty and poor reproduction performance in Nili-Ravi buffaloes.

Resumo A pecuária é uma parte fundamental da indústria agrícola no Paquistão e contribui com 11,53% do PIB nacional. Entre as espécies de gado, os búfalos são considerados o ouro negro do Paquistão. Sendo os maiores produtores de leite em todo o mundo, os búfalos Nili-Ravi são os mais famosos. Os búfalos são afetados por muitas doenças endêmicas, entre as quais a "septicemia hemorrágica" (SH). Este estudo busca verificar os efeitos da exposição experimental de P. multocida B:2 (oral) e seus imunógenos, ou seja, LPS (oral e intravenoso) e OMP (oral e subcutâneo), nos perfis hormonais reprodutivos em búfalos Nili-Ravi. Amostras de soro repetidas foram coletadas da veia jugular de animais experimentais por 21 dias (0, 2, 4, 8, 12, 16, 20, 24, 36, 48, 72, 120, 168, 216, 264, 360, 456 e 504 horas). Os ensaios hormonais para determinar as concentrações séricas do hormônio liberador de gonadotrofina (GnRH), hormônio foliculoestimulante (FSH), hormônio luteinizante (LH), estrogênio (E2) e progesterona (P4) foram realizados usando kits comerciais Elisa (MyBioSource). O perfil hormonal de todos os grupos de tratamento das novilhas bubalinas apresentou variações significativas (P < 0,05) em relação ao grupo controle (G-1). Esses resultados indicam supressão no perfil hormonal reprodutivo de búfalos Nili-Ravi na exposição a P. multocida B:2 e seus imunógenos. Essa influência garante que a exposição à SH possa ser uma possível razão para o atraso da puberdade e o baixo desempenho reprodutivo em búfalos Nili-Ravi.

Uterine microbial ecology and disease in cattle: A review.

Due to the critical contribution of the uterine-associated microbiota in reproductive health, physiology, and performance, culture-independent methods have been increasingly employed to unravel key aspects of microbial ecology in the uterus of cattle. Nowadays, we know that bacterial diversity is crucial to maintain uterine health, however, there is still no consensus on the exact composition of a healthy uterine microbiota (or eubiosis). Generally, loss of bacterial diversity (or dysbiosis) contributes to the development of uterine infections, associated with increased relative abundances of Bacteroides, Fusobacterium, Trueperella, and Porphyromonas. Uterine infections are highly prevalent and gravely influence the profitability of cattle operations, animal welfare, and public health. Thus, understanding the dynamics of uterine microbial ecology is essential to develop effective strategies focused on preventing and mitigating the adverse effects of uterine dysbiosis as well as assisting in the process of restoring the core, healthy uterine microbiota. The aim of this review is to summarize research conducted in the microbial ecology of bovine uteri. We discuss the origin of the uterine microflora of healthy cows and the factors influencing its composition. In addition, we review the biology of specific pathogens that are known to increase in abundance during the occurrence of uterine disease. Lastly, we provide an overview of the bacterial biofilm in the bovine endometrium, and we briefly summarize the rationale for the use of probiotics to prevent uterine disease in cattle.

Sphingosine-1-phosphate mediates FSH-induced cell viability but not steroidogenesis in bovine granulosa cells.

Follicle-stimulating hormone (FSH) stimulates the proliferation, survival, and estradiol synthesis of granulosa cells by binding to their G protein-coupled receptors. Although FSH activates sphingosine kinase-1 (SPHK1) to induce sphingosine-1-phosphate (S1P) synthesis, which is required to mediate the proliferative and survival effect of this gonadotrophin, the mechanisms, and the role of S1P in estradiol synthesis have not been reported. This study aimed to evaluate the importance of FSH-induced S1P synthesis as a mediator of the effects of this gonadotrophin on granulosa cell viability and steroidogenesis and to determine if FSH-induced S1P synthesis depends on estradiol, cAMP, PKA, or PKC. To achieve these objectives, we tested the effects of FSH, a sphingosine kinase-1 inhibitor (SKI-178), estradiol and inhibitors of aromatase, cAMP, PKA, and PKC (Formestane, MDL-12330A, H-89 dihydrochloride hydrate and Calphostin C respectively), on granulosa cell viability, S1P and estradiol production, and the mRNA expression of CYP19A1 and STAR in four in vitro culture experiments. The addition of FSH (1 ng/mL) increased (P < 0.05) granulosa cells number and S1P concentration in the culture media. Conversely, the addition of SKI-178 (10 µM) reduced (P < 0.05) S1P concentration negating the effect of FSH on cell viability. Inhibition of PKC and PKA, but not cAMP, reduced (P < 0.05) S1P secretion of FSH treated granulosa cells. It is important to note that the reduction in S1P secretion was strong (49 %) with the use of the PKC inhibitor. The use of formestane (10 µg) did not modify (P > 0.05) S1P secretion in FSH-treated cells; however, the addition of 5 or 10 ng/mL of estradiol increased (P < 0.05) S1P secretion. Finally, FSH increased (P < 0.05) estradiol concentration in the culture media, but this effect was not blocked by the inhibition of S1P synthesis. Similarly, FSH, SKI-178 or their combination did not modify the mRNA expression of CYP19A1 and STAR. In conclusion, S1P synthesis is stimulated FSH in granulosa cells and mediated mainly by PKC. S1P in turn promotes the granulosa cell viability, however, this does not influence estradiol synthesis. Additionally, estradiol synthesis induced by FSH is not essential for S1P synthesis, however high estradiol concentration may stimulate S1P production by granulosa cells.

Disposable and instrument-free nucleic acid lateral flow cassette for rapid and on-site identification of adulterated goat milk.

Species identification has become a significant concern due to the growing use of food alternatives that may cause allergies and reduce nutritional value. To address the issue of fraudulent adulteration of goat milk products with cow milk, we have developed an affordable, portable, and user-friendly platform called microfluidic-integrated nucleic acid lateral flow strips (LFS). This platform enables simultaneous detection of components derived from both goats and cows in goat milk. In this study, we have introduced an innovative nucleic acid labeling method. The loop primers of loop-mediated isothermal amplification (LAMP) have been modified with amplification terminator spacer C3 and an oligonucleotide sequence, thus eliminating the requirement for costly antibodies in traditional nucleic acid LFS. This modification not only lowers costs but also enables multiple detections. Additionally, we have integrated the LAMP and LFS assay steps into a microfluidic chip, allowing convenient on-site detection while effectively preventing aerosol contamination of LAMP products. The testing process includes rapid DNA extraction, followed by a short nucleic acid addition and incubation for visualized results in about 50 min. This platform is user-friendly, requiring no specialized equipment or extensive training, making it suitable for rapid on-site detection of dairy products by personnel in diverse fields.

Catchment-scale rapid transfer of livestock pharmaceuticals under Mediterranean climate.

Various pharmaceuticals are essential for livestock farming, but some are highly toxic to aquatic life if they reach surface water bodies. Mediterranean Climate is characterized by dry summers followed by intense autumn storms. We studied the effect of these climatic conditions on the risk of pharmaceutical residues transfer to streams at the catchment-scale. Pharmaceutical products routinely used in the study area, as well as their application frequency and season, were identified through interviews with farmers. As a proof a concept, three veterinary pharmaceuticals (Fenbendazole (FBZ), Mebendazole (MBZ) and Ivermectin (IVM)) were chosen as model chemicals based on their relatively high usage, their specificity to represent different types of livestock (swine, sheep and cattle), and their ability to be analyzed using the same analytical method. Stream water was analyzed during low flow periods and at high frequency (up to 2 h-1) during flood events. The selected veterinary pharmaceuticals were not detected during low flow, but FBZ and MBZ reached high concentrations for short periods during floods. Due to the event-driven nature of their transfer, a significant load of veterinary pharmaceuticals can reach the river and cause temporary but significant degradation of water quality (e.g. for FBZ, the water concentration reached up to 355 times the predicted no effect concentration (PNEC)). This indicates that special care should be taken to avoid keeping freshly treated livestock on pastures that may become hydrologically connected under wet conditions. In addition, it suggests that low-frequency monitoring is not sufficient to detect those high concentration levels that exist during very short periods.

Evaluation of two beef cow fixed-time AI protocols that utilize presynchronization.

Presynchronization was evaluated as a method to improve estrus response before fixed-time AI (FTAI). The objective was to compare FTAI results in beef cows from two different presynchronization approaches. Blood samples were collected on Day -14 (Day 0 = CIDR removal) to determine progesterone concentration (≥1 ng/mL = high, <1 ng/mL = low). In a subset (n = 1289), an additional blood sample was collected between Day -21 and -29 to determine cyclicity (if both the Day -14 and Day -21 to -29 samples were classified as low progesterone cows were classified as noncycling). Cows (n = 1388) from 30 herds were grouped by days postpartum (DPP) and age, and randomly assigned to either of two protocols. Cows assigned to the PG 6-day CIDR & FTAI protocol (PG6d) received prostaglandin F2α (PG) on Day -9, CIDR insertion and GnRH on Day -6, and CIDR removal and PG on Day 0. Cows assigned to the 7&7 Synch protocol (7&7) were administered PG and CIDR insertion on Day -14, GnRH on Day -7, and CIDR removal and PG on Day 0. For both protocols, FTAI occurred concurrently with GnRH 66 h after second PG. Pregnancy was determined by transrectal ultrasonography 30-40 d after FTAI. The GLIMMIX procedure of SAS was used to detect differences in estrus response and pregnancy success with herd as a random variable. Estrus response (0-66 h) was analyzed with two models, one included cyclicity and another replaced cyclicity with progesterone concentration at Day -14. In both models, cows assigned to the 7&7 protocol had greater (P < 0.01) estrus response than cows assigned to the PG6d protocol. The model including cyclicity, estrus response was impacted by the cyclicity by DPP interaction (P = 0.03), cyclicity by protocol interaction (P = 0.04), and the tendency of BCS by protocol interaction (P = 0.08). In the estrus response model that included progesterone concentration at Day -14, significant variables included the protocol by progesterone concentration at Day -14 (P = 0.01), and BCS (P < 0.01), while DPP (P = 0.08) and progesterone concentration at Day -14 (P = 0.07) were tendencies. Pregnancy success was influenced by estrual status (P < 0.01), body condition score (P = 0.04), and cycling status (P = 0.02), but was not influenced by protocol (P = 0.75; PG6d = 38 ± 5% and 7&7 = 37 ± 5%). In conclusion, effectiveness of presynchronization method depended on a cows' physiological status, and the 7&7 protocol increased estrus response compared with PG6d, but there was no difference in pregnancy success.

Comparative metabolomic analysis and antigenicity comparison of cow milk and enzymatically treated cow milk.

BACKGROUND: Amino acids (AAs) are important protein building blocks that play a critical role in the function of the immune system. However, comprehensive comparative metabolomics and antigenicity analyses of cow milk (CM) and enzymatically treated CM are relatively scarce. This study analyzed the AAs in the CM and Flavourzyme-treated milk groups (FT), and their antigenicity was also explored. RESULTS: Overall, 50 AAs were detected in the CM and FT groups, with 23 significantly different AAs. The interaction network of these significantly different AAs was analyzed, and 34 significantly different metabolic pathways were found to be involved. It was also found that the antigenicity of the FT group was significantly reduced in comparison with that of the CM group. CONCLUSION: These results enhance our understanding of AAs and antigenicity regarding CM and FT, and provide new ideas and directions for the development of high-quality hypoallergenic dairy products. © 2023 Society of Chemical Industry.

Tracking the binding site of anticancer drug fluxoridin with Fe-related proteins to achieve intelligent drug delivery.

In cancer cells that need a lot of iron for growth and metastasis, halo-transferrin (TF-containing iron) enters the cell with the help of the transferrin receptor 1 (TFR1) protein. If the anticancer drug can bind to the iron site by interacting with apo-transferrin (iron-free FT), it can enter the cancer cell by the same mechanism. Two iron-related proteins, Bovine liver catalase (BLC) and apo-Transferrin (TF), that are important in cancer patients were selected and their interaction with the anti-cancer drug Floxuridine (FUDR) was investigated. Here, the protective role of FUDR was evaluated by several variables such as drug concentration, interaction time, and temperature-induced degradation of enzyme function. The results showed that the protective effect of the FUDR is greater in high concentrations (in 5 × 10-5 M:1.78 % and 2.59 % after 24 and 48 h). The interaction of the FUDR with both proteins can reduce the intensity of the fluorescence emission by a static mechanism. The binding strength of the FUDR with both proteins was almost similar and with the order of 104 M-1 (Kb = 3.90 ± 0.41 × 104 M-1 for BLC-FUDR and 5.01 ± 0.36 × 104 M-1 for TF-FUDR at 310 K). The thermodynamic calculations (in agreement with the docking results) indicated that FUDR-protein complex formation was exothermic and the main binding forces in the binding process were van der Waals interactions and hydrogen bonds. Both fluorophores tryptophan (Trp) and tyrosine (Tyr) of both proteins had significant roles in fluorescence quenching and the interaction process, the polarity of their microenvironment changed. CD results showed that the secondary structure changes of TF are slightly more than BLC. Molecular docking showed that the binding of the FUDR to TF is very close to the Fe-specific site and is placed in the cavity among the wrapping domain, N-Terminal arm, and ß-barrel in BLC.

Study of relations between chemical, colour and fluorescence properties of raw and dried meat powders of cow and yak (Bos grunniens).

Gluteus medius, Longissimus thoracis, and Semitendinosus muscles from the cow and yak (Bos grunniens) reared in the Kyrgyz Republic were dried by convective (hot air) drying and freeze drying. The dried muscles were grinded into fine powders and along with raw muscles were evaluated for water activity (aW), chemical composition (moisture, fat, protein), colour characteristics (L, a, and b values), fluorescence of intrinsic fluorophores (tryptophan, vitamin A, and riboflavin). Processing of measured data tables using common components and specific weights analysis (CCSWA) showed close relations among the chemical, colour, and fluorescence data. CCSWA discriminated muscles depending on chemical composition, animal type, and drying technique applied based on the chemical properties, colour characteristics, and fluorescence spectra. The freeze drying was approved as a preferable dehydration technique comparing with convective drying as the one causing less discoloration to meat. Partial Least Squares Regression (PLSR) models developed using fluorescence spectra allowed accurate quantitative predicting of water activity (aW), moisture, fat, and protein contents, and colour characteristics (L, a, and b values).

Shiga toxin-producing Escherichia coli (STEC) in meat and leafy greens available in the Swedish retail market - Occurrence and diversity of stx subtypes and serotypes.

Shiga toxin-producing Escherichia coli (STEC) is a major cause of foodborne illness, ranging from mild diarrhea to permanent kidney failure. This study summarizes the results of four surveys performed at different time periods, which investigated the occurrence and characteristics of STEC in beef, lamb and leafy greens available in the Swedish retail market. Such data is required when assessing the public health risk of varying types of STEC in different foods, and for establishing risk management measures. Samples from domestic and imported products were collected based on their availability in the retail market. The occurrence of STEC was investigated in 477 samples of beef, 330 samples of lamb and 630 samples of leafy greens. The detection of virulence genes (stx1, stx2, eae) was performed using real-time PCR followed by the isolation of bacteria from stx-positive enriched samples using immunomagnetic separation or an immunoblotting method. All STEC isolated from the food samples was further characterised in terms of stx subtyping and serotyping through whole genome sequencing. STEC was isolated from 2 to 14 % of beef samples and 20 to 61 % of lamb samples, depending on the region of origin. STEC was not isolated from samples of leafy greens, although stx genes were detected in 11 (2 %) of the samples tested. In total, 5 of the 151 sequenced STEC isolates from meat contained stx2 and eae, of which 4 such combinations had the stx2a subtype. The stx2 gene, stx2a in particular, is strongly associated with serious disease in humans, especially in combination with the eae gene. The isolates belonged to 20 different serotypes. Two isolates from beef and one from lamb belonged to the serotype O157:H7 and contained genes for stx2 and eae. Overall, several combinations of stx subtypes were found in isolates from beef, whereas stx1c, either alone or together with stx2b, was the dominant combination found in STEC from lamb. In conclusion, STEC was rare in whole meat samples of domestic beef in the Swedish retail market, whereas such bacteria were frequently found in minced meat and whole meat samples of imported beef and domestic and imported lamb. Although the number of isolates containing genes linked to an increased risk of severe disease was low, beef and lamb in the Swedish retail market is a common source of human exposure to potentially pathogenic STEC.

Canola meal as a supplement for grass-fed beef cattle: Effects on growth rates, carcase and meat quality, and consumer sensory evaluations.

The current study examined the growth rates, carcase characteristics, meat quality, and consumer sensory evaluation of the longissimus lumborum muscle (striploin) from steers that were supplemented with either canola meal or grain-based pellets. Forty Angus and Hereford × Angus steers received one of these two supplements with ad libitum lucerne hay for 60 d prior to slaughter. Average daily weight gain was not affected by dietary treatment; however, hot standard carcase weight was significantly lower for steers offered canola meal compared with steers on the grain-based pellets. Dietary treatment did not affect the carcase characteristics, meat quality traits, and consumer sensory evaluation, irrespective of ageing periods. Therefore, canola meal can be used as an approved Pasturefed Cattle Assurance System (PCAS) supplement on moderate dry quality forages without negatively affecting carcase and meat quality traits.

Enzymatic construction Au NPs-rGO based MIP electrochemical sensor for adulteration detection of bovine-derived allergen in camel milk.

In this work, a high-performance molecularly imprinted polymer (MIP) sensor for the determination of ß-lactoglobulin (ß-LG) was fabricated by using trypsin as a template removal reagent. Gold nanoparticles (Au NPs) and reduced graphene oxide (rGO) designed for electrode modification accelerate the heterogeneous electron transfer rate to enhance the sensitivity of the prepared sensor. With enzymatic hydrolysis, ß-LG templates were effectively digested into short peptides without damage to the MIP so that the imprinted cavities of the MIP were preserved with a complete spatial structure exhibiting high selectivity. Based on the optimization of the protein removal time and pH, the prepared MIP electrochemical sensor could recognize ß-LG in the range of 4-100 ng/mL with a low detection limit (3.58 ng/mL). The sensor also expressed excellent selectivity and was successfully applied to real sample detection. The results demonstrate that the proposed MIP electrochemical sensor may be a promising candidate for camel milk adulteration detection.

Synthesis and characterisation of Manuka and rosemary oil-based nano-entities and their application in meat.

Manuka (MO) and rosemary oils (RO) -containing nanoemulsions and nanocapsules made of sodium alginate and whey protein, were designed and compared for their antioxidant effect. Manuka oil-nanoemulsions and nanocapsules had smaller particle sizes (343 and 330 nm), less negative zeta potential (-12 mV and -10 mV), higher phenolic content, and antiradical characteristics than RO-nano-entities. However, nano-entities of both oils showed more thermostability and sustained release than free oils. Further, the antioxidant effect of essential oils and their nano-entities was compared against sodium nitrite (SN)-added and without antioxidants-added (controls) and Wagyu and crossbred beef pastes (14 days refrigerated storage). No significant difference among MO, RO and their nano-entities was noticed in crossbred pastes, while in Wagyu, nanoemulsions showed the lowest oxidation values than controls and SN-added pastes. Hence, nano-entities can be alternatives to chemical preservatives as natural antioxidants in meat preservation, along with improved thermal stability and release than free oils.

Preparation of food-grade EDC/NHS-crosslinked gelatin nanoparticles and their application for Pickering emulsion stabilization.

Herein, a safe desolvation and crosslinking method was developed to prepare food-grade bovine bone gelatin (BBG) nanoparticles for Pickering emulsion stabilization. The nanoparticle-like structures were formed by adjusting pH 9.0 and adding ethanol, and then stable nanoparticles were formed by using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) as crosslinker. Compared with other pH (2.5, 5.0, 7.0, and 12.0), pH 9.0 was the appropriate pH to prepare BBG nanoparticles. Individual nanoparticles (6.50 nm in height), oligomeric nanoparticles (13.42-22.52 nm in height), and polymeric nanoparticles (obvious liquid-precipitate separation) were formed at EDC·HCl/NHS concentrations of 6, 9-12, and 15-20 mg/mL, respectively. The oligomeric nanoparticles induced the highest emulsion creaming stability. The emulsion creaming ability increased with the increase of BBG nanoparticle concentrations. Low NaCl concentration (e.g., 100 mmol/L) could increase the emulsion creaming stability. Finally, 4 °C was the best storage temperature for fish oil-loaded Pickering emulsions.

Designing tannic acid-polyethyleneimine-modified electrode and novel affinity peptide for ß-lactoglobulin detection in milk.

Harmful substances that cause food allergies can pose a significant threat to consumers along with food safety. According to the World Health Organization (WHO), approximately 10 % of the global population is currently affected by food allergies. Therefore, there is an urgent need for the development of more accurate and precise biosensors capable of detecting these hazardous substances including beta-lactoglobulin. Although numerous detection and analysis methods have been developed, they still suffer from various limitations. In this study, a tannic acid-polyethyleneimine (TA-PEI) network modified screen-printed electrodes (SPE) are newly developed and the binding sequence of peptide against ß-LG was successfully screened using random peptide library. A novel affinity peptide with the desired sequence of S-L-S-P-S-L-W-Q-V-S-M-L-G-G-G-G-E-P-L-Q-L-K-M against ß-lactoglobulin (ß-LG) is designed and synthesized. The synthesized affinity peptide was immobilized on TA-PEI modified SPE to develop peptide-based sensor against ß-LG for the first time. Under successful optimization, the developed sensor exhibited a linear relationship between 50 and 750 ng, with a Kd of 213.9 ng. In addition, the sensor was able to detect ß-LG in cow and goat milk, with average recoveries of 88.5 % and 92.2 %, respectively.

The use of olive cake in the diet of dairy cows improves the mineral elements of Provola cheese.

Mineral elements (Ca, Na, K, Mg, Zn, Ti, Sr, Fe, Ni, Ba, Cr, Mn, Cu, Se, Cd, Mo, B, V, As, Pb and Hg) in Provola cheeses obtained from dairy cows fed with two different integrated diets (Biotrak) and without olive cake (Control) were determined to discriminate between the two different cheeses. The results showed that cheeses from the Biotrak group presented higher values of essential elements. Selenium (Se) was found to be the most interesting: in Biotrak cheeses the content of Se was in the range of 0.112 to 0.281 mg/kg, about twice the content of Se in cheeses from the Control group. Among the toxic elements, only Cd was found in the samples, but at low levels (in average lower than 0.11 mg/kg). Therefore, the use of olive cake in animal feed is a good strategy to improve the mineral profile of the product obtained.

Effect of static magnetic field-assisted thawing on the quality, water status, and myofibrillar protein characteristics of frozen beef steaks.

This study investigated the effect of static magnetic field-assisted thawing (SMAT) at varying intensities (0, 1, 2, and 3 mT) on the quality, water status, and myofibrillar protein (MP) characteristics of frozen beef steaks. The thawing times of SMAT-1, 2, and 3 treatments could be shortened by approximately 10.9 %, 20.0 %, and 8.5 %, respectively, compared to the control. The results indicated that SMAT treatment significantly decreased thawing loss, maintained color stability, and reduced the degree of lipid oxidation in beef steaks compared to the control group (P < 0.05). Low-field nuclear magnetic resonance results confirmed that SMAT treatment enhanced the water-holding capacity of muscle. Furthermore, SMAT-2 treatment protected the muscle microstructure, decreased carbonyl content, and increased total sulfhydryl content (P < 0.05) compared to the control group. In conclusion, SMAT treatment effectively improved the beef quality and the characteristics of MP after thawing, especially in 2 mT.

A ratiometric fluorescence platform for on-site screening meat freshness.

Meat freshness is related to food safety and human health. Developing a simple and effective method for on-site detection of meat freshness is essential to ensure food safety. This study aimed to explore a ratiometric fluorescence platform for on-site screening of meat freshness. We synthesized a series of benzothiazole-based fluorescent compounds (BM, BHM and BTH), each with different recognition groups for detecting meat freshness biomarkers cadaverine (Cad) and putrescine (Pte). The optimized 2-(2'-hydroxyphenyl-3-aldehyde-5-1,3-indanedione) benzothiazole (BTH) demonstrated a noticeable color and fluorescence change, a fast response (<15 min), and high selectivity and sensitivity (LOD = 70 nM) to Cad. Portable test strips based on BTH were prepared for rapid visual detection of meat freshness, which exhibited visible color and fluorescen color changes to Cad and Pte. Furthermore, a portable smartphone-based fluorescence device integrated with a self-programmed Python program was fabricated and used on-site to monitor Cad and Pte within 5 min. The BTH-loaded portable test strips were successfully employed as low-cost, high-contrast, fast-response, and smartphone-adaptable fluorescent labels for detecting Cad and Pte in meat samples under different temperatures (25 °C, 4 °C, and -20 °C). This enabled consumers and food supply chain stakeholders to quickly and visually monitor the meat freshness in real beef, chicken, and pork products.

Authenticity identification of animal species in characteristic milk by integration of shotgun proteomics and scheduled multiple reaction monitoring (MRM) based on tandem mass spectrometry.

Milk is one of the oldest natural dairies with high value, which has different species including cow, camel, donkey, goat, sheep, buffalo, yak and et al. However, economically motivated adulteration of non-cow milk with cheaper cow milk occurs frequently. To develop a high-throughput approach for milk species authentication, integration of shotgun proteomics and scheduled multiple reaction monitoring (MRM) was developed. In total, 37 specific peptides were screened as unique to different species. Specific peptides processing stability was investigated under different treatment (heat, pressure, fermentation). Subsequently, four quantitative ion pairs of peptides from cow milk and six quantitative ion pairs of peptides from six non-cow milks were selected for the adulteration quantitative analysis. The method is capable of detection adulteration in the range of 1%-100%, and the quantitative recoveries ranged from 91.07% to 111.75%. The results suggested that combination of shotgun proteomics and MRM had potential for the milk species authentication.