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1.
Estima (Online) ; 19(1): e0121, jan.-dez. 2021.
Artículo en Portugués | BDENF - Enfermería | ID: biblio-1151133

RESUMEN

Objetivo:avaliar a qualidade de vida de pessoas com úlcera venosa e correlacionar com as características clínicas e sintomas associados à ferida. Método: estudo quantitativo, transversal e analítico. Para as análises das associações das variáveis qualitativas foram aplicados os testes Qui-quadrado e exato de Fisher, e para as variáveis quantitativas, o coeficiente de correlação de Spearman. Utilizou-se o instrumento Pressure Ulcer Scale for Healing para a avaliação da ferida e o Freiburg Life Quality Assessment Wound-wk para análise da qualidade de vida. Fizeram parte do estudo 103 sujeitos com tempo de feridas superior a seis semanas, de etiologia venosa. Resultados: houve correlação das características clínicas da úlcera venosa e sintomas associados com a qualidade de vida. Nas dimensões sintomas físicos, pessoas com úlcera venosa apresentraram pior escore em relação ao sintoma dor (p<0,0001). Associadas a isso, as características clínicas como a aparência (p< 0,0001) e a área (p=0,0037) da ferida também manifestaram correlação. Em relação à escala de avaliação da ferida (área, tecido na ferida e exsudato), o pior escore interferiu nos sintomas físicos. Conclusão: a dor, tamanho da ferida e seu aspecto influenciam negativamente na qualidade de vida de pessoas com úlcera venosa


Asunto(s)
Calidad de Vida , Úlcera Varicosa , Cicatrización de Heridas , Enfermería
2.
Int J Pharm Compd ; 25(2): 100-103, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33798108

RESUMEN

Healthy human skin performs a constellation of functions essential to good health, and the consequences of disruption to that effective external defense system have been recognized since antiquity. The earliest treatments for dermal injuries and diseases were compounded: They were prepared for each patient and could be modified to address progressive phases of healing. The benefits of that therapeutic approach continue today, made immeasurably more effective by modern pharmaceutical compounding. In this article, which is the first of several in a series that presents healing-drug profiles, various agents that can be compounded to enable dermal healing are described. Those drugs are not commercially available in the most safe and effective combinations at the time of this writing, but a skilled compounding pharmacist can incorporate compatible agents in preparations designed to ensure best outcomes. Formulations that promote dermal healing are provided for easy reference.


Asunto(s)
Preparaciones Farmacéuticas , Cicatrización de Heridas , Composición de Medicamentos , Humanos , Farmacéuticos , Piel
3.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(2): 629-632, 2021 Apr.
Artículo en Chino | MEDLINE | ID: mdl-33812442

RESUMEN

Mesenchymal stem cells (MSC) are capable of supporting hematopoiesis, regulating immune responses, promoting tissue regeneration and homing to damaged tissues, but their efficacy cannot completely exploit due to various factors. Studies in recent years have shown that the biological characteristics of mesenchymal stem cells have plasticity. Researchers can enhance the biological performance of MSC by pretreatment with hypoxia, bioactive molecules, genetic modification, and mechanical stimulation, as well as adjusting MSC transplantation strategies, which has great significance for promoting the transformation of MSC. Therefore, in this review, the recent research advances in the plasticity of the biological characteristics of MSC are summarized briefly.


Asunto(s)
Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas , Cicatrización de Heridas
4.
Acta Cir Bras ; 36(3): e360303, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33825787

RESUMEN

PURPOSE: This study aimed to elaborate a hydrogel constituted by carboxymethyl chitosan (CMC), hyaluronic acid (HA) and silver (Ag) and to evaluate its healing effect on partial-thickness burn wounds experimentally induced in rats. METHODS: CMC was obtained by chitosan reacting with monochloroacetic acid. The carboxymethylation was confirmed by Fourier-transform infrared spectroscopy and hydrogen nuclear magnetic resonance (NMR). Scanning electron microscopy was used to determine the morphologicalcharacteristics of chitosan and CMC. After the experimental burn wound induction, the animals (n = 126) were treated with different CMC formulations, had their occlusive dressings changed daily and were followed through 7, 14 and 30 days. Morphometric, macroscopic and microscopic aspects and collagen quantification were evaluated. RESULTS: Significative wound contraction, granulation tissue formation, inflammatory infiltration and collagen fibers deposit throughout different phases of the healing process were observed in the CMC hydrogels treated groups. CONCLUSIONS: The results showed that, in the initial phase of the healing process, the most adequate product was the CMC/HA/Ag association, while in the other phases the CMC/HA association was the best one to promote the healing of burn wounds.


Asunto(s)
Quemaduras , Quitosano , Animales , Quemaduras/tratamiento farmacológico , Colágeno , Hidrogeles , Ratas , Cicatrización de Heridas
5.
Medicine (Baltimore) ; 100(14): e25395, 2021 Apr 09.
Artículo en Inglés | MEDLINE | ID: mdl-33832132

RESUMEN

ABSTRACT: Skin grafts are not suitable for closing tendon- or bone-exposing wounds, which require flap surgery. Dermal regeneration templates have value for closing such wounds, but the disadvantages of the technique include implantation failures because of infection, hematoma formation, or inappropriate immobilization. Negative-pressure wound therapy was reported to increase graft acceptance in difficult wounds.This retrospective case series of 65 patients evaluated negative-pressure therapy combined with artificial dermis for the treatment of acute or chronic tendon- or bone-exposing wounds. The artificial dermis was placed after adequate wound-bed preparation, with simultaneous application of a vacuum-assisted closure system. Split-thickness skin grafting was performed after the implanted artificial dermis had become established.The overall success rate was 88.1% (59/67): 88.6% (39/44) in the chronic wounds group and 87% (20/23) in the acute-trauma group separately. The overall mean survival time of artificial dermis in success cases was 13.24 ±â€Š7.14 days. In separately, the survival time of artificial dermis had no statistically difference in chronic wound group (13.64 ±â€Š7.53 vs 12.60 ±â€Š5.86. P = .943), but had significant statistical difference in acute trauma group (12.45 ±â€Š6.44 days vs 23.33 ±â€Š4.04 days, P = .018). Also, comorbidity of PAOD was found a strong risk factor of failure in chronic wound group (100% vs 23.1%, P < 0.001).We concluded that artificial dermis combined with negative-pressure therapy followed by split-thickness skin grafting might be a reliable and effective option for surgical reconstruction of tendon- or bone-exposing wounds, and could decreasing waiting periods of autologous skin graft.


Asunto(s)
Terapia de Presión Negativa para Heridas/métodos , Trasplante de Piel/métodos , Piel Artificial/normas , Heridas y Traumatismos/cirugía , Adulto , Anciano , Arteriopatías Oclusivas/complicaciones , Arteriopatías Oclusivas/epidemiología , Autoinjertos/trasplante , Estudios de Casos y Controles , Terapia Combinada/efectos adversos , Terapia Combinada/métodos , Comorbilidad , Femenino , Humanos , Masculino , Persona de Mediana Edad , Terapia de Presión Negativa para Heridas/efectos adversos , Enfermedad Arterial Periférica/complicaciones , Enfermedad Arterial Periférica/epidemiología , Procedimientos Quirúrgicos Reconstructivos/métodos , Estudios Retrospectivos , Factores de Riesgo , Trasplante de Piel/efectos adversos , Piel Artificial/efectos adversos , Colgajos Quirúrgicos/trasplante , Tendones/patología , Tendones/cirugía , Resultado del Tratamiento , Cicatrización de Heridas/fisiología , Heridas y Traumatismos/complicaciones , Heridas y Traumatismos/patología
6.
Wiad Lek ; 74(1): 112-117, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33851599

RESUMEN

OBJECTIVE: The aim of the study is to reveal in the experiment the morphological features of the infected skin wounds healing, which are a manifestation of acne vulgaris severe and very severe forms, using a gel with carbon dioxide extract of hops. PATIENTS AND METHODS: Materials and methods: An experimental study was carried out on 80 male WAG rats of three months of age. The animals were divided into 9 groups. Group 1 consisted of intact animals (n=6). Group 2 was represented by animals (n=6), which had hair epilation on the dorsal surface of the body in an area of 1 сm2, followed by application of 2 ml of placebo gel to this area. Group 3 included animals (n=6), which were epilated on the dorsal surface of the body in an area of 1 сm2 and applied 2 ml of 1 % gel with carbon dioxide extract of hops. Group 4 included rats (n=6), which were epilated on the dorsal surface of the body in an area of 1 сm2 and simulated thermal damage. Group 5 was represented by 10 rats, who were epilated on the dorsal surface of the body in an area of 1 сm2, simulated thermal damage, followed by applying 2 ml of placebo gel to the wound surface. Group 6 included rats (n=10), who underwent measures similar to group 5, followed by application of 2 ml of 1 % gel with carbon dioxide extract of hops to the wound surface. In group 7, there were 6 rats, which were epilated on the dorsal surface of the body in an area of 1 сm2, thermal damage to the skin with underlying soft tissues was simulated, followed by application to the wound surface the reference strains of Staphylococcus aureus, Streptococcus pyogenes, Proteus vulgaris, Propionibacterium acnes, Escherichia coli, Pseudomonas aeruginosa, Malassezia slooffiae, Malassezia pachydermatis, Candida albicans, Candida parapsilosis. In groups 8 and 9, there were 15 rats each, which underwent measures similar to group 7, followed by applying 2 ml of placebo gel on its surface on the next day after infection of the wound in group 8, and in group 9 - 2 ml of 1 % gel with carbon dioxide extract hops. The material for the study was the skin with underlying soft tissues. It was used histological, histochemical, morphometric and statistical methods. RESULTS: Results: This experimentally created gel with carbon dioxide extract of hops activates separation processes of horny masses from the surface of the epidermis, cleaning the pores of the skin; stimulating the proliferative activity of the epidermis, which is located in the marginal sections of the wound or covers the surface of the regenerate; activating the processes of cleansing the wound from necrotic tissue; activating the growth and maturation of granulation tissue with its subsequent transformation into connective tissue. It has anti-inflammatory, bactericidal and antimycotic effects, normalizing skin microbiocenosis. CONCLUSION: Conclusions: The complex morphological study has showed that gel with carbon dioxide extract of hops is a highly effective drug in treatment of severe and very severe acne vulgaris, characterized by the development of deep and infected wound defects.


Asunto(s)
Acné Vulgar , Humulus , Infección de Heridas , Animales , Dióxido de Carbono , Malassezia , Masculino , Ratas , Cicatrización de Heridas
7.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 35(4): 496-501, 2021 Apr 15.
Artículo en Chino | MEDLINE | ID: mdl-33855836

RESUMEN

Objective: To review the clinical research progress of mesenchymal stem cells (MSCs) in the treatment of chronic wounds. Methods: The literature related to the chronic wound repair with MSCs at home and abroad in recent years was extensively reviewed, and the possible mechanism of MSCs in the treatment of chronic wounds, as well as its application and existing problems were summarized. Results: MSCs can participate in all aspects of chronic wound healing to promote wound healing, and has shown broad application prospects in clinical trials. MSCs commonly used in clinical research include bone marrow-derived MSCs, adipose-derived tissue MSCs, and umbilical cord-derived MSCs. Conclusion: MSCs treatment is a promising strategy for the chronic wounds, but there are still many problems in its widespread clinical application that require further research.


Asunto(s)
Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas , Cordón Umbilical , Cicatrización de Heridas
8.
Angiol Sosud Khir ; 27(1): 24-32, 2021.
Artículo en Ruso | MEDLINE | ID: mdl-33825725

RESUMEN

AIM: The study was aimed at comparing efficacy of conventional conservative therapy and comprehensive treatment including a plasmid VEGF-165-gene therapy drug in 'no-option' chronic limb-threatening ischaemia with different prevalence of trophic ulcers and infection during a 1-year follow-up period. PATIENTS AND METHODS: A total of 101 patients (54% being men and 46% women, mean age 69 years) with 'no-option' chronic limb-threatening ischaemia underwent comprehensive conservative treatment. They were subdivided into 4 groups according to the WIFI classification: WIFI 130 (n=38), 131 (n=23), 230 (n=16), 231 (n=24). The control group patients (n=58) received standard treatment using a PGE1 analogue (Vasaprostan) and the study group patients (n=43) underwent standard conservative treatment (SCT) in combination with gene therapy. The end points of the study were as follows: major amputation rate, amputation-free survival, total mortality, and ulcer healing rate during a 1-year of follow up. RESULTS: Major amputation rate in the control and study groups amounted to 35 and 28% (p=0.48), respectively, with amputation-free survival of 53 and 63% (p=0.35), total mortality of 21 and 12% (p=0.23), ulcer healing rate of 31 and 51% (p=0.04), respectively. The WIfI classification made it possible to single out a subgroup of patients (WIfI combination 130) yielding other statistically significant results: major amputation rate 27% and 0% (p=0.03), amputation-free survival 59 and 94% (p=0.025), ulcer healing rate 50 and 88% (p=0.016), respectively. CONCLUSION: Using plasmid-based VEGF-165 gene therapy in the subgroup with the WIfI combination 130 decreases the major amputation rate (p=0.03), increases amputation-free survival (p=0.025) and promotes ulcer healing (p=0.016) compared with the standard therapy during 1-year follow up. No significant differences in the compared groups were revealed by all endpoints of the study for other combinations analysed. The total mortality rate in patients with limb-threatening ischaemia does not depend on either the initial severity of ulcer or the selcted methods of conservative treatment.


Asunto(s)
Tratamiento Conservador , Recuperación del Miembro , Anciano , Amputación , Femenino , Humanos , Isquemia/cirugía , Estimación de Kaplan-Meier , Masculino , Valor Predictivo de las Pruebas , Estudios Retrospectivos , Factores de Riesgo , Factores de Tiempo , Resultado del Tratamiento , Cicatrización de Heridas
9.
Bone Joint J ; 103-B(4): 755-761, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33789474

RESUMEN

AIMS: This study sought to compare the rate of deep surgical site infection (SSI), as measured by the Centers for Disease Control and Prevention (CDC) definition, after surgery for a fracture of the hip between patients treated with standard dressings and those treated with incisional negative pressure wound therapy (iNPWT). Secondary objectives included determining the rate of recruitment and willingness to participate in the trial. METHODS: The study was a two-arm multicentre randomized controlled feasibility trial that was embedded in the World Hip Trauma Evaluation cohort study. Any patient aged > 65 years having surgery for hip fracture at five recruitment centres in the UK was considered to be eligible. They were randomly allocated to have either a standard dressing or iNPWT after closure of the wound. The primary outcome measure was deep SSI at 30 and 90 days, diagnosed according to the CDC criteria. Secondary outcomes were: rate of recruitment; further surgery within 120 days; health-related quality of life (HRQoL) using the EuroQol five-level five-dimension questionnaire (EQ-5D-5L); and related complications within 120 days as well as mobility and residential status at this time. RESULTS: A total of 462 valid randomizations were carried out (232 and 230 in the standard dressing and iNPWT groups, respectively). In the standard dressing group, 14 of 218 patients (6.4%) developed deep SSI. In the iNPWT group, four of 214 patients (1.9%) developed deep SSI. This gives a total rate of SSI of 4.2% (95% confidence interval (CI) 2.7% to 6.5%). Patients and surgeons were willing to participate in the study with 462 patients being recruited from a possible 749 (62.3%). CONCLUSION: The rate of deep SSI 30 days after surgery for a fracture of the hip was 4%, which makes a study comparing the clinical effectiveness of standard dressings and iNPWT feasible. Cite this article: Bone Joint J 2021;103-B(4):755-761.


Asunto(s)
Vendajes , Fracturas de Cadera/cirugía , Terapia de Presión Negativa para Heridas , Infección de la Herida Quirúrgica/prevención & control , Cicatrización de Heridas , Anciano , Anciano de 80 o más Años , Estudios de Factibilidad , Femenino , Humanos , Masculino , Reino Unido
10.
Adv Exp Med Biol ; 1310: 495-507, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33834447

RESUMEN

Skin is vulnerable to various external insults such as burn, severe injury, or inflammation, which necessitates a better strategy for wound repair. Mesenchymal stem cells (MSCs) can self-renew and differentiate into various supporting tissues including cartilage, bone, muscle, and adipose tissue. Along with their unique multipotent capacity, they secrete various paracrine mediators such as growth factors, cytokines, and membrane-enclosed particles called extracellular vesicles (EVs). Herein, we discussed the general traits of EVs such as cell-to-cell communicator, and highlighted the recent preclinical outcomes, with a focus on the application of MSC-derived EVs in wound repair. This chapter provides insights into developing novel strategies for skin wound healing in a cell-free manner.


Asunto(s)
Vesículas Extracelulares , Células Madre Mesenquimatosas , Citocinas , Humanos , Inflamación , Cicatrización de Heridas
11.
Bone Joint J ; 103-B(3): 456-461, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33641428

RESUMEN

AIMS: To clarify the effectiveness of the induced membrane technique (IMT) using beta-tricalcium phosphate (ß-TCP) for reconstruction of segmental bone defects by evaluating clinical and radiological outcomes, and the effect of defect size and operated site on surgical outcomes. METHODS: A review of the medical records was conducted of consecutive 35 lower limbs (30 males and five females; median age 46 years (interquartile range (IQR) 40 to 61)) treated with IMT using ß-TCP between 2014 and 2018. Lower Extremity Functional Score (LEFS) was examined preoperatively and at final follow-up to clarify patient-centered outcomes. Bone healing was assessed radiologically, and time from the second stage to bone healing was also evaluated. Patients were divided into ≥ 50 mm and < 50 mm defect groups and into femoral reconstruction, tibial reconstruction, and ankle arthrodesis groups. RESULTS: There were ten and 25 defects in the femur and tibia, respectively. Median LEFS improved significantly from 8 (IQR 1.5 to 19.3) preoperatively to 63.5 (IQR 57 to 73.3) at final follow-up (p < 0.001). Bone healing was achieved in all limbs, and median time from the second stage to bone healing was six months (IQR 5 to 10). Median time to bone healing, preoperative LEFS, or postoperative LEFS did not differ significantly between the defect size groups or among the treatment groups. CONCLUSION: IMT using ß-TCP provided satisfactory clinical and radiological outcomes for segmental bone defects in the lower limbs; surgical outcomes were not influenced by bone defect size or operated part. Cite this article: Bone Joint J 2021;103-B(3):456-461.


Asunto(s)
Trasplante Óseo/métodos , Fosfatos de Calcio/farmacología , Fémur/cirugía , Ilion/trasplante , Procedimientos Quirúrgicos Reconstructivos/métodos , Tibia/cirugía , Adulto , Artrodesis , Desbridamiento , Femenino , Fémur/lesiones , Fémur/patología , Humanos , Japón , Masculino , Persona de Mediana Edad , Polimetil Metacrilato , Estudios Retrospectivos , Tibia/lesiones , Tibia/patología , Cicatrización de Heridas/efectos de los fármacos
12.
Zhonghua Shao Shang Za Zhi ; 37(2): 191-195, 2021 Feb 20.
Artículo en Chino | MEDLINE | ID: mdl-33648331

RESUMEN

Exosomes are a kind of membrane vesicle with a diameter of 30-150 nm. It is formed by the budding of multiple vesicles in cells, which can fuse with the cell membrane and be released into the extracellular matrix. Adipose derived stem cells (ADSCs) have the potential of self-renewal and multi-directional differentiation. They can transport the active substance, regulate the inflammatory response, cell migration, proliferation, differentiation and angiogenesis via the action of paracrine exosomes, so as to enhance the ability of wound repair, promote wound healing, and inhibit the formation of scars. Chronic wounds refer to the wounds that can not reach the anatomic and functional integrity through the normal, orderly, and timely repair process, and the course of the wound healing is more than 4 weeks. At present, there are various treatment methods for chronic wounds, among which ADSCs, although showing a good application prospect, have some limitations due to ethical issues, while exosomes can avoid this problem. This article reviews the treatment of chronic wounds with ADSC exosomes.


Asunto(s)
Exosomas , Células Madre Mesenquimatosas , Adipocitos , Tejido Adiposo , Células Madre , Cicatrización de Heridas
13.
Carbohydr Polym ; 260: 117767, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712125

RESUMEN

Wound healing is a dynamic and intricate process, and newly dressings are urgently needed to promote wound healing over the multiple stages. Herein, two water-soluble adenine-modified chitosan (CS-A) derivatives were synthesized in aqueous solutions and freeze-dried to obtain porous sponge-like dressings. The novel derivatives displayed antibacterial activities against S. aureus and E. coli. Moreover, CS-A derivatives demonstrated excellent hemocompatibility and cytocompatibility, as well as promoted the proliferation of the wound cells by shortening the G1 phase and improving DNA duplication efficiency. The ability of CS-A sponges to promote wound healing was studied in a full-thickness skin defect model. The histological analysis and immunohistochemical staining showed that the wounds treated with CS-A sponges displayed fewer inflammatory cells, and faster regeneration of epithelial tissue, collagen deposition and neovascularization. Therefore, CS-A derivatives have potential application in wound dressings and provide new ideas for the design of multifunctional biomaterials.


Asunto(s)
Adenina/química , Materiales Biocompatibles/química , Quitosano/química , Animales , Vendajes , Materiales Biocompatibles/farmacología , Puntos de Control del Ciclo Celular/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Liofilización , Masculino , Ratones , Porosidad , Ratas , Ratas Sprague-Dawley , Piel/efectos de los fármacos , Piel/patología , Cicatrización de Heridas/efectos de los fármacos
14.
Pan Afr Med J ; 38: 23, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33777291

RESUMEN

Fournier's gangrene (FG) is a rapidly progressive necrotizing bacterial dermo-hypodermitis of the perineum and external genitalia. It represents a real medical and surgical emergency requiring multidisciplinary care. Our study was based on the retrospective analysis of 18 cases of FG, collected in the Department of General and Visceral Surgery of Fattouma Bourguiba University Hospital in Monastir over an 18-year period extending from January 2000 to December 2018. Our series included 18 cases of FG collected over an 18-year period, an annual incidence of one case per year. The average age of our patients was 58 years (36 to 77). The male prevalence was clear. Diabetes and old age were found to be the major risk factors. The treatment was based on an aggressive surgical debridement remains to be the cornerstone of therapy and is commonly preceded by patient preparation for the surgical act by perioperative resuscitation and broad-spectrum antibiotic therapy, possibly accompanied by hyperbaric oxygen therapy (HBOT). The vaccum assisted closure (VAC) therapy is also used, which is a non-invasive system that promotes open wound healing. Healing techniques can be once the septic risk is controlled. Dressings topical treatments, such as fatty substances or calcium alginate, in addition to skin grafts, musculo-neurotic or musculo-cutaneous cover flaps can be used. During the follow-up period, no reccurrence occurred in 14 out of the 18 cases (2 patients were lost to follow-up and 2 patients died). A colostomy was closed in 10 out of 11 cases with simple follow-ups. Restorative surgery (partial thickness skin graft) at the perineal level was performed in only one case. Despite the better understanding of its etiopathogenesis, the advent of targeted antibiotic therapy, the establishment of a better codification of surgical procedures, the contribution of hyperbaric oxygenation and reconstruction techniques, mortality rates are still high and FG remains a real health threat, thus constituting a real medical and surgical emergency.


Asunto(s)
Desbridamiento/métodos , Gangrena de Fournier/terapia , Cicatrización de Heridas , Adulto , Anciano , Antibacterianos/administración & dosificación , Vendajes , Terapia Combinada , Femenino , Estudios de Seguimiento , Gangrena de Fournier/patología , Humanos , Oxigenación Hiperbárica/métodos , Masculino , Persona de Mediana Edad , Terapia de Presión Negativa para Heridas/métodos , Estudios Retrospectivos , Factores de Riesgo
15.
Life Sci ; 273: 119271, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33652035

RESUMEN

A growing body of evidence has shown that extracellular vesicles can be efficient as experimental therapeutics in pre-clinical models of skin wounds, but there is a significant unmet need to translate this to clinical utilization. The objectives of the current systematic review were to identify the strength of the therapeutic effects of EVs derived from stem cells in cutaneous wounds and to assess which EV-mediated mechanisms could be involved in the therapeutic response. PubMed, ISI Web of Science, and Scopus databases were systematically searched. We retrieved English-language articles published through June 2020. In vivo studies which applied stem cell-derived EVs were included for further analysis. The Risk of bias was assessed by the SYRCLE tool. We identified thirty-nine pre-clinical studies that evaluated the effects of EVs on the wound healing process. The included studies varied greatly in EVs isolation techniques, route of administration, EVs producing cells, and follow-up time. In vivo application revealed beneficial effects of EVs on accelerating wound closure and re-epithelialization in a dose-dependent manner. Elevated angiogenesis was reported in twelve eligible studies through multiple signaling pathways such as PI3K/Akt, MAPK/ERK, and JAK/STAT. The well-known signaling pathway to inhibit scar formation was TGF-ß2/SMAD2. However, all included studies were not blinded enough which may have introduced bias. Therefore, the transition of EV's efficacy into the clinics is deeply rooted in the following important factors: 1) pre-clinical studies with a lower risk of bias and longer follow-up time, and 2) consistent, reproducible, and feasible manufacturing of EVs production in a large-scale commercial program.


Asunto(s)
Vesículas Extracelulares/trasplante , Piel/lesiones , Células Madre/citología , Cicatrización de Heridas , Heridas y Traumatismos/terapia , Animales , Humanos , Piel/patología
16.
Molecules ; 26(4)2021 Feb 22.
Artículo en Inglés | MEDLINE | ID: mdl-33671791

RESUMEN

Peptide materials have recently been considered for use in various industrial fields. Because of their efficacy, safety, and low cost, therapeutic peptides are studied for various diseases, including atopic dermatitis (AD). AD is a common inflammatory skin disease impairing the patient's quality of life. Various therapies, such as treatments with corticosteroids, calcineurin inhibitors, and antibody drugs, have been applied, but numerous side effects have been reported, including skin atrophy, burning, and infection. In the case of antibody drugs, immunogenicity against the drugs can be a problem. To overcome these side effects, small peptides are considered therapeutic agents. We previously identified the small wound healing peptide AES16-2M with a sequence of REGRT, and examined its effects on AD in this study. Interestingly, the administration of AES16-2M downregulated the AD disease score, ear thickness, serum IgE, and thymic stromal lymphopoietin (TSLP) in AD mice. The thickness of the epidermal layer was also improved by AES16-2M treatment. In addition, quantities of IL-4-, IL-13-, and IL-17-producing CD4 T cells from peripheral lymph nodes and spleens were reduced by injection of AES16-2M. Furthermore, the expression of TSLP was significantly reduced in AES16-2M-treated human keratinocytes. Therefore, these results suggest that AES16-2M can be a novel candidate for AD treatment.


Asunto(s)
Dermatitis Atópica/tratamiento farmacológico , Péptidos/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Línea Celular , Dermatophagoides farinae , Modelos Animales de Enfermedad , Femenino , Humanos , Ratones , Péptidos/síntesis química , Péptidos/química
17.
Carbohydr Polym ; 260: 117808, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712154

RESUMEN

In the present study, a novel synthetic tissue adhesive material capable of sealing wounds without the use of any crosslinking agent was developed by conjugating thermosensitive hexanoyl glycol chitosan (HGC) with gallic acid (GA). The degree of N-gallylation was manipulated to prepare GA-HGCs with different GA contents. GA-HGCs demonstrated thermosensitive sol-gel transition behavior and formed irreversible hydrogels upon natural oxidation of the pyrogallol moieties in GA, possibly leading to GA-HGC crosslinks through intra/intermolecular hydrogen bonding and chemical bonds. The GA-HGC hydrogels exhibited self-healing properties, high compressive strength, strong tissue adhesive strength and biodegradability that were adjustable according to the GA content. GA-HGCs also presented excellent biocompatibility and wound healing effects. The results of in vivo wound healing efficacy studies on GA-HGC hydrogels indicated that they significantly promote wound closure and tissue regeneration by upregulating growth factors and recruiting fibroblasts compared to the untreated control group.


Asunto(s)
Materiales Biocompatibles/química , Quitosano/química , Ácido Gálico/química , Animales , Materiales Biocompatibles/farmacología , Fuerza Compresiva , Hidrogeles/química , Hidrogeles/farmacología , Reología , Porcinos , Adhesivos Tisulares/química , Cicatrización de Heridas/efectos de los fármacos
18.
Science ; 371(6534): 1102-1103, 2021 03 12.
Artículo en Inglés | MEDLINE | ID: mdl-33707253
19.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 35(3): 392-398, 2021 Mar 15.
Artículo en Chino | MEDLINE | ID: mdl-33719251

RESUMEN

Objective: To summary the standardized management in research and applications of platelet derivatives for tissue regeneration. Methods: The related literature about bottlenecks and standardized management of platelet derivatives in recent years was reviewed and analyzed. Results: Although the platelet derivatives are increasingly used to accelerate the regenerative processes of injured joint, skin, nerve, ligament/tendon, and alveolar bone, etc., the large variation in preparation methods, diverse nomenclature, incomplete reporting system, and lack of quantitative and standardized management of the preparation process have caused uncertainty and incomparability of research and application results. In recent years, there has been a trend towards standardized research and management of platelet derivatives. Conclusion: The implementation of standardized research and quality management will contribute to promote the research and application of platelet derivatives in the field of tissue regeneration.


Asunto(s)
Plasma Rico en Plaquetas , Plaquetas , Regeneración Ósea , Tendones , Cicatrización de Heridas
20.
Methods Mol Biol ; 2265: 65-71, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33704705

RESUMEN

Cell migration is a critical process involved in morphogenesis, inflammation, and cancer metastasis. Wound healing assay is a simple, non-expensive, and highly reproducible method to study cancer cell migration in vitro. It is based on the observation that cells growing in a monolayer migrate to re-establish cell contacts after the development of an artificial wound. The assay involves creation of a wound in a monolayer, image acquisition during wound closure, and comparison of migrated area at initial and final time points.


Asunto(s)
Movimiento Celular , Melanoma/patología , Cicatrización de Heridas , Técnicas de Cultivo de Célula/métodos , Línea Celular Tumoral , Movimiento Celular/efectos de los fármacos , Factor de Crecimiento Epidérmico/farmacología , Humanos , Cicatrización de Heridas/efectos de los fármacos
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