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1.
Sci Total Environ ; 753: 141950, 2021 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-32906044

RESUMEN

2,4-dichlorophenoxyacetic acid (2,4-D), a widely used herbicide, is a small organic chemical pollutant in the environment. To develop a nanobody-based immunoassay for monitoring trace levels of 2,4-D, a step-wise strategy for the generation of nanobodies highly specific against this small chemical was employed. Firstly, we synthesized three novel haptens mimicking 2,4-D and assessed their influence on the sensitivity and specificity of the existing antibody-based assay. Polyclonal antibodies (pAb) from rabbits showed good sensitivity and moderate specificity for 2,4-D, pAb from llama based on selected haptens showed similar performance when compared to those from rabbits. Secondly, nanobodies derived from llama were generated for 2,4-D by an effective procedure, including serum monitoring and one-step library construction. One nanobody, NB3-9, exhibited good sensitivity against 2,4-D (IC50 = 29.2 ng/mL) had better specificity than the rabbit pAb#1518, with no cross-reactivities against the 2,4-D analogs tested. Thirdly, one-step fluorescent enzyme immunoassay (FLEIA) for 2,4-D based on a nanobody-alkaline phosphatase (AP) fusion was developed with IC50 of 1.9 ng/mL and a linear range of 0.4-8.6 ng/mL. Environmental water samples were analyzed by FLEIA and LC-MS/MS for comparison, and the results were consistent between both methods. Therefore, the proposed step-wise strategy from hapten design to nanobody-AP fusion production was successfully conducted, and the resulting nanobody based FLEIA was demonstrated as a convenient tool to monitor 2,4-D residuals in the environment.


Asunto(s)
Herbicidas , Agua , Ácido 2,4-Diclorofenoxiacético , Animales , Cromatografía Liquida , Ensayo de Inmunoadsorción Enzimática , Herbicidas/análisis , Conejos , Espectrometría de Masas en Tándem
2.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 34(11): 1446-1453, 2020 Nov 15.
Artículo en Chino | MEDLINE | ID: mdl-33191704

RESUMEN

Objective: To investigate the effect of microencapsulated transgenic bone marrow mesenchymal stem cells (BMSCs) transplantation on early steroid induced osteonecrosis of femoral head (SONFH) in rabbits. Methods: Alginate poly- L-lysine-sodium alginate (APA) microencapsulated transgenic BMSCs with high expression of Foxc2 were prepared by high-voltage electrostatic method. Part of the cells were cultured in osteoblasts and observed by alizarin red staining at 2 and 3 weeks. Forty New Zealand white rabbits were used to prepare SONFH models by using hormone and endotoxin. Thirty two rabbits who were successful modeling were screened out by MRI and randomly divided into 4 groups (groups A, B, C and D, n=8); another 6 normal rabbits were taken as normal control (group E). The rabbits in group A did not receive any treatment; and in groups B, C, and D were injected with normal saline, allogeneic BMSCs, and APA microencapsulated transgenic BMSCs respectively after core decompression. At 6 and 12 weeks after operation, specimens of femoral head were taken for HE staining to observe bone ingrowth; the expressions of osteocalcin (OCN), peroxisome proliferative activated receptor γ 2 (PPARγ-2), and vascular endothelial growth factor (VEGF) proteins were observed by immunohistochemistry staining. At 12 weeks after operation, the bone microstructure was observed by transmission electron microscope, and the maximum compressive strength and average elastic modulus of cancellous bone and subchondral bone were measured by biomechanics. Results: After 2 and 3 weeks of induction culture, alizarin red staining showed the formation of calcium nodules, and the number of calcium nodules increased at 3 weeks when compared with 2 weeks. The rabbits in each group survived until the experiment was completed. Compared with groups A, B, and C, the trabeculae of group D were more orderly, the empty bone lacunae were less, there were abundant functional organelles, and obvious osteogenesis was observed, and the necrotic area was completely repaired at 12 weeks. Immunohistochemical staining showed that, at 6 and 12 weeks after operation, the expressions of OCN and VEGF in groups A, B, and C were significantly lower than those in groups D and E, while those in groups B and C were significantly higher than those in group A, and in group E than in group D ( P<0.05). The expression of PPARγ-2 was significantly higher in groups A, B, and C than in groups D and E, and in group A than in groups B and C, and in group D than in group E ( P<0.05). At 12 weeks after operation, biomechanical test showed that the average elastic modulus and maximum compressive strength of cancellous bone and subchondral bone in groups D and E were significantly higher than those in groups A, B, and C ( P<0.05); there was no significant difference between groups A, B, and C and between groups D and E ( P>0.05). Conclusion: In vivo transplantation of microencapsulated transgenic BMSCs can repair early SONFH in rabbits.


Asunto(s)
Células Madre Mesenquimatosas , Osteonecrosis , Animales , Células de la Médula Ósea , Células Cultivadas , Cabeza Femoral , Osteogénesis , Conejos , Factor A de Crecimiento Endotelial Vascular
3.
Sheng Wu Gong Cheng Xue Bao ; 36(10): 2083-2091, 2020 Oct 25.
Artículo en Chino | MEDLINE | ID: mdl-33169573

RESUMEN

Rabbit haemorrhagic disease virus (RHDV) and myxoma virus (MYXV), are two pathogens that have harmful effect on rabbit breeding and population decline of European rabbits in their native range, causing rabbit haemorrhagic disease (rabbit fever) and myxomatosis, respectively. The capsid protein VP60 of the RHDV represents the major antigenic protein. To develop a recombinant bivalent vaccine candidate that can simultaneously prevent these two diseases, we used the nonessential gene TK (thymidine kinase) of MYXV as the insertion site to construct a recombinant shuttle vector p7.5-VP60-GFP expressing the RHDV major capsid protein (VP60) and the selectable marker GFP. Then the shuttle vector p7.5-VP60-GFP was transfected into rabbit kidney cell line RK13 which was previously infected with MYXV. After homologous recombination, the recombinant virus expressing GFP was screened under a fluorescence microscope and named as rMV-VP60-GFP. Finally, the specific gene-knock in and expression verification of the vp60 and gfp genes of the recombinant virus was confirmed by PCR and Western blotting. The results showed that these two genes were readily knocked into the MYXV genome and also successfully expressed, indicating that the recombinant MYXV expressing the vp60 of RHDV was generated. Protection against MYXV challenge showed that the recombinant virus induced detectable antibodies against MYXV which would shed light on development of the effective vaccine.


Asunto(s)
Infecciones por Caliciviridae , Virus de la Enfermedad Hemorrágica del Conejo , Vacunas Sintéticas , Proteínas Estructurales Virales , Animales , Western Blotting , Infecciones por Caliciviridae/prevención & control , Infecciones por Caliciviridae/veterinaria , Virus de la Enfermedad Hemorrágica del Conejo/genética , Virus de la Enfermedad Hemorrágica del Conejo/inmunología , Conejos , Vacunas Sintéticas/genética , Vacunas Sintéticas/inmunología , Proteínas Estructurales Virales/genética
4.
Acta Cir Bras ; 35(10): e202001001, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33206869

RESUMEN

PURPOSE: Incisional hernia (IH) is a frequent complication of median laparotomy. The use of prophylactic mesh to reduce IH incidence has gained increasing attention. We hypothesized that in an animal model, linea alba prophylactic reinforcement with a three-dimensional T-shaped polypropylene mesh results in greater abdominal wall resistance. METHODS: Study was performed in 27 rabbits. After abdominal midline incision, animals were divided into three groups according to the laparotomy closure method used: (1)3D T-shaped coated mesh; (2)3D T-shaped uncoated mesh; and (3) closure without mesh. After 4 months, each animal's abdominal wall was resected and tensiometric tests were applied. Results included IH occurrence, adhesions to the mesh, and wound complications. RESULTS: There was no significant difference between the groups in maximum tensile strength (p=0.250) or abdominal wall elongation under maximum stress (p=0.839). One rabbit from the control group developed IH (p=1.00). Small intestine and colon adhesions occurred only in the uncoated mesh group (p<0.001) and the degree of adhesions was higher in this group compared to the coated mesh group (p<0.05). CONCLUSION: Use of the current 3D T-shaped prophylactic mesh model did not result in a significant difference in tensiometric measurements when compared with simple abdominal wall closure in rabbits.


Asunto(s)
Pared Abdominal , Hernia Ventral , Hernia Incisional , Pared Abdominal/cirugía , Animales , Hernia Ventral/cirugía , Polipropilenos , Conejos , Mallas Quirúrgicas , Adherencias Tisulares/prevención & control
5.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(10): 1465-1471, 2020 Oct 30.
Artículo en Chino | MEDLINE | ID: mdl-33118515

RESUMEN

OBJECTIVE: To elucidate the pathogenic role of leukotriene B4 (LTB4) in pulmonary hyper-permeability and inflammation induced by lung-protective mechanical ventilation (LPMV) in rabbits. METHODS: Thirty-two healthy Japanese white rabbits were randomized into 4 groups for treatment with vehicle or bestatin (a leukotriene A4 hydrolase inhibitor that inhibits LTB4 production) administered intragastrically at the daily dose of 8 mg/kg for 5 days, followed by sham operation (group S and group BS, respectively, in which the rabbits were anesthetized only) or LPMV (group PM and group BPM, respectively, in which the rabbits received ventilation with 50% oxygen at a tidal volume of 8 mL/kg for 5 h). The concentrations of LTB4 and cyclic adenosine monophosphate (cAMP) in the lung tissues were analyzed by ELISA. cAMP content, protein kinase A (PKA) protein expression and the Rap1-GTP protein to total Rap1 protein ratio were determined to assess the activities of cAMP/PKA and Rap1 signaling pathways. The lung injury was evaluated by assessing lung permeability index, lung wet/dry weight ratio, polymorphonuclear leukocyte (PMN) count in bronchoalveolar lavage fluid (BALF), pulmonary myeloperoxidase (MPO) activity and lung histological scores. RESULTS: None of the examined parameters differed significantly between group S and group BS. All the parameters with the exception of lung histological score increased significantly in group PM and group BPM as compared to those in group S (P < 0.05). Compared with those in PM group, the rabbits in group BPM showed significantly reduced LTB4 production in the lungs (P < 0.05), up-regulated cAMP/ PKA and Rap1 signaling pathway activities (P < 0.05), and alleviated lung hyper-permeability and inflammation (P < 0.05). CONCLUSIONS: LPMV can induce LTB4 overproduction to down-regulate cAMP/PKA and Rap1 signaling pathways in the lungs of rabbits, which results in lung hyper-permeability and inflammation. Bestatin can inhibit LTB4 production in the lungs to protect against LPMV-induced lung hyper-permeability and inflammation.


Asunto(s)
Lesión Pulmonar , Animales , Líquido del Lavado Bronquioalveolar , Leucotrieno B4 , Pulmón , Lesión Pulmonar/etiología , Lesión Pulmonar/prevención & control , Neutrófilos , Conejos , Respiración Artificial/efectos adversos
6.
Arch Virol ; 165(12): 2847-2856, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-33034764

RESUMEN

Here, we investigated the fecal, oral, blood, and skin virome of 10 laboratory rabbits using a viral metagenomic method. In the oral samples, we detected a novel polyomavirus (RabPyV), and phylogenetic analysis based on the large T antigen, VP1 and VP2 regions indicated that the novel strain might have undergone a recombination event. Recombination analysis based on related genomes confirmed that RabPyV is a multiple recombinant between rodent-like and avian-like polyomaviruses. In fecal samples, three partial or complete genome sequences of viruses belonging to the families Picobirnaviridae, Parvoviridae, Microviridae and Coronaviridae were characterized, and phylogenetic trees were constructed based on the predicted amino acid sequences of viral proteins. This study increases the amount of genetic information on viruses present in laboratory rabbits.


Asunto(s)
Metagenoma , Poliomavirus/aislamiento & purificación , Conejos/virología , Proteínas Virales/genética , Virus/clasificación , Animales , Animales de Laboratorio/virología , Sangre/virología , Heces/virología , Genoma Viral , Boca/virología , Filogenia , Piel/virología , Virus/aislamiento & purificación , Secuenciación Completa del Genoma
7.
Int J Nanomedicine ; 15: 7215-7234, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33061377

RESUMEN

Background: With excellent shape memory and superelastic properties, shape memory alloy (SMA) is an ideal actuator, and it can form smart structure for different applications in medical field. However, SMA devices cause apparent thermal damage to the surrounding tissues when it works in vivo, making the application of smart structure that is composed of SMA actuator in vivo is greatly limited. Methods: In this paper, coating (APA) with PLA as the main body to limit the heat conduction, a multifunctional Ag nanoparticles (AgNPs)/polylactic acid (PLA)/Al2O3 was synthesized. The Al2O3 layer was formed by micro-arc oxidation (MAO) and AgNPs were synthesized by silver nitrate and ethylene glycol. Scanning electron microscopy, transmission electron microscope, and Fourier transform infrared spectra were applied to analyze the morphology and characterization of APA coating. The antimicrobial activity, thermal insulation activity, and biocompatibility of APA coating were furtherly explored and verified through animal experiments and immunohistochemistry. Results: With different particle sizes and concentrations of AgNPs, APA multi-functional films were successfully prepared. The Al2O3 layer was closely combined with SMA and formed a porous surface, so the PLA and AgNPs layers can firmly adhere to SMA, thus reducing the release of nickel ions in SMA. AgNPs gave APA coating excellent antibacterial activity and effectively inhibited the growth of Staphylococcus aureus. In addition, coupled with the low thermal conductivity of PLA and Al2O3, AgNPs were tightly anchored on the surface of PLA, which has high infrared reflectivity, making the APA coating obtain good thermal insulation performance. Conclusion: We have successfully prepared the APA coating and obtained the optimum amount of AgNPs, which makes it have good thermal insulation performance, good antibacterial activity and good biocompatibility, which provides a new prospect for the application of SMA.


Asunto(s)
Antibacterianos/farmacología , Materiales Biocompatibles Revestidos/farmacología , Níquel/farmacología , Temperatura , Titanio/farmacología , Óxido de Aluminio/farmacología , Animales , Línea Celular , Nanopartículas del Metal/química , Nanopartículas del Metal/ultraestructura , Ratones , Pruebas de Sensibilidad Microbiana , Poliésteres/farmacología , Conejos , Plata/farmacología , Staphylococcus aureus/efectos de los fármacos
8.
Arthroscopy ; 36(10): 2708-2709, 2020 10.
Artículo en Inglés | MEDLINE | ID: mdl-33039042

RESUMEN

It was thought that the meniscus together with articular cartilage would be among the first tissues to successfully engineer. However, despite extensive research in the field, this path was proven not to be easy. Tissue engineering of musculoskeletal tissues remains promising, and several advancements have further identified the role of biological treatments in meniscus repair and regeneration. To move forward, and advance from being promising to being forthcoming, an important step would be to improve the quality of presented studies. This editorial commentary proposes a checklist of necessary reported data for tissue engineering studies: macroscopic and histologic appearance, tissue composition, mechanical properties, and translational data.


Asunto(s)
Cartílago Articular , Menisco , Animales , Cartílago Articular/cirugía , Humanos , Menisco/cirugía , Conejos , Regeneración , Células Madre , Ingeniería de Tejidos
9.
Biol Res ; 53(1): 48, 2020 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-33081840

RESUMEN

BACKGROUND: Psoriasis is a common chronic inflammatory skin disease. Keratinocytes hyperproliferation and excessive inflammatory response contribute to psoriasis pathogenesis. The agents able to attenuate keratinocytes hyperproliferation and excessive inflammatory response are considered to be potentially useful for psoriasis treatment. Daphnetin exhibits broad bioactivities including anti-proliferation and anti-inflammatory. This study aims to evaluate the anti-psoriatic potential of daphnetin in vitro and in vivo, and explore underlying mechanisms. METHODS: HaCaT keratinocytes was stimulated with the mixture of IL-17A, IL-22, oncostatin M, IL-1α, and TNF-α (M5) to establish psoriatic keratinocyte model in vitro. Cell viability was measured using Cell Counting Kit-8 (CCK-8). Quantitative Real-Time PCR (qRT-PCR) was performed to measure the mRNA levels of hyperproliferative marker gene keratin 6 (KRT6), differentiation marker gene keratin 1 (KRT1) and inflammatory factors IL-1ß, IL-6, IL-8, TNF-α, IL-23A and MCP-1. Western blotting was used to detect the protein levels of p65 and p-p65. Indirect immunofluorescence assay (IFA) was carried out to detect p65 nuclear translocation. Imiquimod (IMQ) was used to construct psoriasis-like mouse model. Psoriasis severity (erythema, scaling) was scored based on Psoriasis Area Severity Index (PASI). Hematoxylin and eosin (H&E) staining was performed to examine histological change in skin lesion. The expression of inflammatory factors including IL-6, TNF-α, IL-23A and IL-17A in skin lesion was measured by qRT-PCR. RESULTS: Daphnetin attenuated M5-induced hyperproliferation in HaCaT keratinocytes. M5 stimulation significantly upregulated mRNA levels of IL-1ß, IL-6, IL-8, TNF-α, IL-23A and MCP-1. However, daphnetin treatment partially attenuated the upregulation of those inflammatory cytokines. Daphnetin was found to be able to inhibit p65 phosphorylation and nuclear translocation in HaCaT keratinocytes. In addition, daphnetin significantly ameliorate the severity of skin lesion (erythema, scaling and epidermal thickness, inflammatory cell infiltration) in IMQ-induced psoriasis-like mouse model. Daphnetin treatment attenuated IMQ-induced upregulation of inflammatory cytokines including IL-6, IL-23A and IL-17A in skin lesion of mice. CONCLUSIONS: Daphnetin was able to attenuate proliferation and inflammatory response induced by M5 in HaCaT keratinocytes through suppression of NF-κB signaling pathway. Daphnetin could ameliorate the severity of skin lesion and improve inflammation status in IMQ-induced psoriasis-like mouse model. Daphnetin could be an attractive candidate for future development as an anti-psoriatic agent.


Asunto(s)
Adyuvantes Inmunológicos , Antiinflamatorios , Imiquimod , Inflamación , Psoriasis , Umbeliferonas , Adyuvantes Inmunológicos/efectos adversos , Animales , Antiinflamatorios/farmacología , Proliferación Celular , Humanos , Imiquimod/efectos adversos , Inflamación/tratamiento farmacológico , Queratinocitos , Ratones , Ratones Endogámicos BALB C , Psoriasis/inducido químicamente , Psoriasis/tratamiento farmacológico , Conejos , Umbeliferonas/farmacología
10.
Am J Vet Res ; 81(11): 843-848, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33107754

RESUMEN

OBJECTIVE: To investigate use of a candidate maxillary nerve block in rabbits. ANIMALS: 13 healthy New Zealand White rabbits (Oryctolagus cuniculus). PROCEDURES: In phase 1, the maxillary nerve block procedure was performed in 7 sedated rabbits with 2 volumes (0.25 and 0.5 mL) of a saline (0.9% NaCl)-tissue marker dye solution (1 injection/side by random assignment). Rabbits were euthanized and dissected; numeric scales were used to rate injection accuracy and extent of staining. In phase 2, the nerve block was performed with articaine hydrochloride-epinephrine solution (0.5 mL) on a randomly assigned side in 6 sedated rabbits, with the contralateral side used as a control. Sensory function of the relevant dermatome was tested in triplicate with an algesiometer 0, 30, and 90 minutes after recovery from sedation. Statistical methods were used to compare results between injection volumes (phase 1) and between treated and control sides (phase 2). RESULTS: In phase 1, dye was in contact with the targeted nerve after 13 of 14 injections. Accuracy and extent of staining did not differ significantly between volumes. In phase 2, algesiometer-applied force tolerance differed significantly between treated and control sides 30 minutes after recovery from sedation (56 to 145 minutes after the nerve block procedure). No adverse effects were detected in either study phase. CONCLUSIONS AND CLINICAL RELEVANCE: The described technique for a maxillary nerve block was accurate and effective for desensitization of the relevant dermatome as assessed by algesiometry in healthy rabbits. Additional studies are needed to assess use of this procedure in rabbits of other breeds and its efficacy for clinical use. (Am J Vet Res 2020;81:843-848).


Asunto(s)
Nervio Maxilar , Bloqueo Nervioso , Animales , Inyecciones/veterinaria , Bloqueo Nervioso/veterinaria , Conejos
11.
Altern Lab Anim ; 48(3): 127-135, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-33006498

RESUMEN

The complement system consists of at least 50 proteins that serve as one of the first lines of defence against foreign, or damaged, cells and invading microorganisms. Its dysregulation underlies the pathophysiology of many different diseases, which makes functional assays of complement activity crucial; they are, however, underutilised. Standard haemolysis assays for the analysis of complement function employ sensitised non-human erythrocytes (e.g. from the sheep, guinea-pig or rabbit), the use of which raises animal welfare concerns. To provide an alternative to the use of such animal-derived products for complement function assays, we developed a method that employs modified human erythrocytes to evaluate the activity of complement pathways. Human erythrocytes were subjected to various chemical and/or proteolytic treatments involving 2,4,6-trinitrobenzene sulphonate (TNBS) and pancreatin. Haemolysis assays demonstrated that sequential treatment with TNBS and pancreatin resulted in significantly greater complement-mediated haemolysis, as compared to TNBS or pancreatin treatment alone. Evidence that lysis of the modified erythrocytes was complement-mediated was provided by the chelation and subsequent restoration of calcium in the plasma. Thus, such modified human erythrocytes could be used as an alternative to animal-derived erythrocytes in haemolysis assays, in order to evaluate complement activity in human plasma during, for example, the screening of patients for complement deficiencies and other abnormalities in a clinical setting.


Asunto(s)
Activación de Complemento , Hemólisis , Animales , Proteínas del Sistema Complemento , Eritrocitos/inmunología , Cobayas , Humanos , Conejos , Ovinos
12.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 414-417, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-33018016

RESUMEN

Atrial Fibrillation (AF) is most common sustained cardiac arrhythmia and a precursor to many fatal cardiac conditions. Catheter ablation, which is a minimally invasive treatment, is associated with limited success rates in patients with persistent AF. Rotors are believed to maintain AF and core of rotors are considered to be robust targets for ablation. Recently, multiscale entropy (MSE) was proposed to identify the core of rotors in ex-vivo rabbit hearts. However, MSE technique is sensitive to intrinsic parameters, such as scale factor and template dimension, that may lead to an imprecise estimation of entropy measures. The purpose of this research is optimize MSE approach to improve its accuracy and sensitivity in rotor core identification using simulated EGMs from human atrial model. Specifically, we have identified the optimal time scale factor (τopt) and optimal template dimension (Τopt) that are needed for efficient rotor core identification. The τopt was identified to be 10, using a convergence graph, and the Τopt (~20 ms) remained the same at different sampling rates, indicating that optimized MSE will be efficient in identifying core of the rotor irrespective of the signal acquisition system.


Asunto(s)
Fibrilación Atrial , Ablación por Catéter , Animales , Fibrilación Atrial/cirugía , Técnicas Electrofisiológicas Cardíacas , Entropía , Atrios Cardíacos , Humanos , Conejos
13.
Vestn Oftalmol ; 136(6): 19-25, 2020.
Artículo en Ruso | MEDLINE | ID: mdl-33084275

RESUMEN

Palpebral eyelid weight implants are currently considered as the gold standard method for lagophthalmos correction. Manufactured from different precious metals, they have both benefits and drawbacks. The article presents the results of an experimental morphological study of biointegration of different eyelid weight implants used for correction of paralytic lagophthalmos. PURPOSE: To investigate biointegration properties of implants made of gold, platinum and platinum-gold alloy. MATERIAL AND METHODS: The study enrolled 4 Chinchilla rabbits (8 eyelids). Palpebral implants manufactured of gold, platinum and platinum-gold alloy were placed into the eyelids of the experimental animals. The morphological examination was performed 1 and 6 months after the implantation using paraffin sections. RESULTS: Light microscopy revealed that the implant beds were surrounded by pronounced fibrovascular capsule of uneven thickness over the whole follow-up. The capsule formed after the placement of an implant made of gold and platinum alloy was more loose. However, the emerging connective tissue bridges of the capsule contributed to its immobilization in the tissues and lower risk of extrusion. No signs of an inflammatory and/or allergic reaction were observed in any of the cases. CONCLUSION: The obtained results show that implants made of a 90/10% platinum-gold alloy and 999.9 fine platinum have similar biointegration in terms of morphological features during 6 months of the follow-up period.


Asunto(s)
Enfermedades de los Párpados , Parálisis Facial , Animales , Enfermedades de los Párpados/etiología , Enfermedades de los Párpados/cirugía , Párpados/cirugía , Parálisis Facial/cirugía , Prótesis e Implantes , Implantación de Prótesis , Conejos
14.
Arkh Patol ; 82(5): 42-49, 2020.
Artículo en Ruso | MEDLINE | ID: mdl-33054031

RESUMEN

OBJECTIVE: To determine the prognostic value of the expression of Ki-67, p53, and Notch1 in the diagnosis of prostate cancer. MATERIAL AND METHODS: Surgical and diagnostic materials obtained from patients with benign prostatic hyperplasia, prostate intraepithelial neoplasia, and prostate cancer of different grades underwent studies; a total of 98 patients were examined. Cell antigens were determined using rabbit anti-Ki-67 monoclonal antibodies (Clone SP6), mouse anti-p53 monoclonal antibodies (Clone Y5), and recombinant anti-Notch1 antibodies (Clone EP1238Y). RESULTS: Comparing Ki-67, p53, and Notch1 expression indices revealed statistically significant differences. There was a significant direct correlation between Ki-67 and p53 expression indices and prostate cancer grade groups based on the morphological Gleason grading system (rs=0.736; p<0.001; rs=0.682; p=0.035, respectively) and a strong inverse correlation between Notch1 expression and prostate cancer grade groups (rs= -0.425, p<0.001). CONCLUSION: Notch1 expression in conjunction with the low proliferative activity of cells and the absence of p53 (Y5) protein may suggest that the mechanism of apoptosis is preserved in tumor cells, which expands indications for medical treatment of prostate cancer.


Asunto(s)
Apoptosis , Neoplasia Intraepitelial Prostática , Neoplasias de la Próstata , Animales , Humanos , Antígeno Ki-67/genética , Masculino , Ratones , Pronóstico , Neoplasias de la Próstata/genética , Conejos , Receptor Notch1/genética , Proteína p53 Supresora de Tumor/genética
15.
PLoS One ; 15(10): e0239317, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33027299

RESUMEN

The present study demonstrated a noninvasive preocular sensor system for the concurrent monitoring of diabetes and one of its prevalent complications, dry eye syndrome (DES), using tear fluids. Two distinct sensors, i.e., the glucose and DES sensors, were prepared and encased together in a single housing unit to produce the sensor system, and the tip was designed to be in contact with the eye surface noninvasively to collect and deliver tear fluid to the sensors. The glucose sensor was modified from a commercially available electrochemical sensor to allow for the measurement of glucose concentrations, even in a small amount of collected tear fluid. The DES sensor was equipped with a microchannel spaced with two parallel electrodes to determine the amount of collected tear fluid. In vivo experimental results revealed that with the collected tear fluid of about 0.6-1.0 µl, the sensor system estimated the blood glucose concentrations with acceptable accuracy compared with that of the glucometer in clinical use. The DES condition in animals was diagnosed with high sensitivity (91.7%) and specificity (83.3%).


Asunto(s)
Técnicas Biosensibles/métodos , Glucemia/análisis , Síndromes de Ojo Seco/diagnóstico , Lágrimas/química , Animales , Área Bajo la Curva , Técnicas Biosensibles/instrumentación , Electrodos , Masculino , Curva ROC , Conejos
16.
Int J Nanomedicine ; 15: 7937-7949, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33116510

RESUMEN

Background: Cyclosporine A (CsA) is an exceptional immunosuppressant used for the treatment of immune disorders. Niosomal vesicles are promising drug carriers that are formed by self-association of nonionic surfactants and cholesterol in an aqueous phase. The objective of the study was to formulate combined nonionic surfactant based vesicles and to evaluate their in vitro characterization, release studies and in vivo studies. Materials and Methods: Five niosomal formulations (F7 to F11) were prepared using the thin film hydration method. The molar ratio of cholesterol and non-ionic surfactant taken was 1:1. In formulation F10, the combination of surfactants Span 20 and Brij 35 was used. The niosomes were characterized by zeta sizer and SEM for particle size analysis, in vitro drug release and stability studies. The pharmacokinetic studies were conducted on healthy albino rabbits. Results: The size of niosome was found in the range of 427.1 nm to 972.3 nm. SEM image of optimized formulations F10 exhibit the spherical nature of niosomal vesicles. DSC thermograms of niosomal formulations exhibited a broadened endothermic peak. The stability study exhibited that all formulations are stable and negligible change of vesicle size and entrapment was observed with time. The percentage drug release was significantly higher as compared to CsA plain dispersion for all niosomal formulations at pH 1.2 and 7.4. The release kinetic behavior showed that all preparations were best described by zero order and can release active ingredient in a sustained manner. The pharmacokinetic data showed the test formulation (F10) possessed greater bioavailability as compared to the reference formulation (CsA aqueous dispersion). Conclusion: The formulation F10 demonstrated a comparatively more delayed rate of release with enhanced dissolution as compared to a single surfactant scheme. The F10 formulation can be a remarkable nanotechnology for prolonged delivery of CsA orally with improved dissolution profile and bioavailability.


Asunto(s)
Portadores de Fármacos/química , Inmunosupresores/química , Inmunosupresores/farmacología , Tensoactivos/química , Animales , Disponibilidad Biológica , Colesterol/química , Ciclosporina/administración & dosificación , Ciclosporina/química , Ciclosporina/farmacocinética , Ciclosporina/farmacología , Liberación de Fármacos , Inmunosupresores/administración & dosificación , Inmunosupresores/farmacocinética , Liposomas , Tamaño de la Partícula , Conejos
17.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 5220-5223, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-33019161

RESUMEN

The aim of this study is to design an implantable Lower Esophageal Sphincter (LES) stimulator connected and controlled by an Android Bluetooth for the treatment of the gastroesophageal reflux disease (GERD). Then the animal experiments are carried out to evaluate the function of the system. The LES stimulator is composed of an external controller, an Android application (APP) via a smart phone and an implantable electronic device (IED). The external controller is designed to receive the settings parameters information sent by the Android APP via a Bluetooth module, and then is programmed to generate specific electrical stimulation pulses to the LES. The Android APP controls the start and stop of stimulation and the settings of stimulation parameters. The in vivo IED consists of a bipolar stimulating lead, a bipolar head connector and a receiving module. The bipolar stimulating lead is constructed of biocompatible materials: platinum-iridium electrodes which are coated with parylene and an outer silicone rubber sheathing. The size of the receiving module has been significantly decreased to 20×20×2 mm3, which is packaged by polydimethylsiloxane (PDMS) and proposed to deliver stimulation pulses from the external controller to the implantable lead. The one-month implantation experiment on rabbits has been performed to evaluate the LES stimulator. The results indicate that the proposed LES stimulator meets the requirements of the functions, effectiveness and safety.


Asunto(s)
Terapia por Estimulación Eléctrica , Reflujo Gastroesofágico , Animales , Estimulación Eléctrica , Esfínter Esofágico Inferior , Reflujo Gastroesofágico/terapia , Prótesis e Implantes , Conejos
18.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 5244-5247, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-33019167

RESUMEN

This report describes a novel therapeutic technique called photo-mediated ultrasound therapy (PUT). PUT applies synchronized short pulse duration (nanosecond) laser and ultrasound burst on targeted tissue, offering high-precision localized treatment. PUT is based on controlled induction and promotion of micro-cavitation activity in the target tissue. PUT is able to safely and effectively treat retinal neovascularization in rabbits with persistent nonperfusion up to 4 weeks after PUT in the choroidal vasculature.Clinical Relevance- PUT can selectively remove retinal angiogenesis in clinically-relevant disease models in humansized eyes (rabbit) without damaging surrounding tissue.


Asunto(s)
Neovascularización Retiniana , Terapia por Ultrasonido , Animales , Coroides , Rayos Láser , Neovascularización Patológica , Conejos , Neovascularización Retiniana/terapia
19.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 4204-4207, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-33018924

RESUMEN

The gastrointestinal (GI) tract is in part controlled by slow wave electrical activity. Recordings of slow waves with high-resolution (HR) electrode arrays are used to characterize normal and abnormal conduction pathways. Improving the quality of these electrical recordings is important for developing a better understanding of abnormal activity. Contact pressure is one factor that can affect the quality of electrical recordings. We compared the performance of two pressure sensing devices for measuring HR electrode array contact pressure. A Velostat-based sensor array was custom designed and built in a 4 × 2 conguration (area: 30 mm2 per sensor) to be integrated into electrical recordings. Commercially available FlexiForce A201 sensors were used to compare to the Velostat-based sensors. Benchtop testing of these sensors was performed; the error of the Velostat-based sensors (14-31%) was better than that of the FlexiForce sensors (20-49%) within a range of 2666-6666 Pa. The Velostat-based sensors were also more repeatable than the FlexiForce sensors over the same pressure range. Simultaneous pressure and slow wave recordings were performed in vivo on a rabbit small intestine. The Velostat-based sensors were able to resolve spatiotemporal changes in contact pressure in the range of 0-10 000 Pa.


Asunto(s)
Microbioma Gastrointestinal , Tracto Gastrointestinal , Animales , Electricidad , Electrodos , Intestino Delgado , Conejos
20.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 4212-4216, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-33018926

RESUMEN

Frequent and accurate monitoring of intraocular pressure is an important aspect of glaucoma management and is central to timely therapeutic intervention and treatment optimization. Intraocular pressure is known to fluctuate not only throughout the day, but also as a function of the heart rate. This pulsatory pressure change behavior is known as the ocular pulse. In this study, we report on the measurement of the ocular pulse profile using a miniaturized intraocular pressure sensor implanted in the eye of a New Zealand White rabbit. The pressure sensor is based on the principle of interferometry and does not require an internal power source. The ocular pulse variation has been measured up to 5 Hz with an accuracy of +/- 0.15 mmHg using both a DSLR reader and a handheld smartphone reader.


Asunto(s)
Glaucoma , Presión Intraocular , Animales , Ojo , Glaucoma/diagnóstico , Frecuencia Cardíaca , Conejos , Tonometría Ocular
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