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1.
Nihon Yakurigaku Zasshi ; 157(5): 352-355, 2022.
Artículo en Japonés | MEDLINE | ID: mdl-36047152

RESUMEN

The epidermal growth factor receptor (EGFR) is the most extensively examined receptor tyrosine kinase. Several EGFR mutations and modifications have been shown to induce self-activation, which plays a central role in carcinogenesis. Recently, environmental chemicals such as PM2.5 can also activate EGFR and become risk factors for cancer. Although, the detailed mechanism remains unknown. In this study, we focused on 1,2-naphthoquinone (1,2-NQ) which is a secondary metabolite of naphthalene. Humans are exposed to 1,2-NQ through the combustion of fossil and diesel fuel and from tobacco smoke and PM2.5. Here, we demonstrate that 1,2-NQ is a novel EGFR-specific activator. We found that 1,2-NQ forms a covalent bond called N-arylation with EGFR Lys80 which is in the extracellular domain by LC-MS/MS. This modification activates the EGFR-Akt signaling pathway, which inhibits serum deprivation-induced apoptosis in A549 cells. Our study reveals an original mode of EGFR activation via covalent binding. We propose the correlation between EGFR activation without ligands and environmental pollutant-associated diseases such as cancer.


Asunto(s)
Contaminantes Ambientales , Cromatografía Liquida , Contaminantes Ambientales/toxicidad , Receptores ErbB/genética , Receptores ErbB/metabolismo , Humanos , Naftoquinonas , Material Particulado , Fosforilación , Transducción de Señal , Espectrometría de Masas en Tándem
2.
Eur J Dermatol ; 32(3): 305-311, 2022 05 01.
Artículo en Inglés | MEDLINE | ID: mdl-36065535

RESUMEN

Short- and long-term exposure to atmospheric pollution has significant health effects. The skin is the organ directly in contact with pollutants and is responsible for protection of the organism. Particulate matter (PM) such as polycyclic aromatic hydrocarbons (PAHs) are the basis of certain pulmonary as well as dermatological complications. Pollution exacerbates certain illnesses such as atopic dermatitis and cancer, and it may also participate in delaying wound healing and in the occurrence of chronic ailments such as diabetes. The aryl hydrocarbon receptor (AhR) transcription factor, at the core of these responses to pollutants, is expressed by all cells of the skin. The AhR is subject to tight regulation that depends on its ligand. Pollutants act in a deleterious manner via the AhR, influencing the behaviour of keratinocytes as well as fibroblasts. Natural ligands, on the other hand, allow the noxious effects of pollution to be countered. This non-systematic review of the literature shows that modulation of AhR appears to be an excellent therapeutic approach to improve or stop the cutaneous problems linked to pollution.


Asunto(s)
Dermatitis Atópica , Contaminantes Ambientales , Receptores de Hidrocarburo de Aril , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Dermatitis Atópica/metabolismo , Contaminantes Ambientales/toxicidad , Humanos , Queratinocitos/efectos de los fármacos , Queratinocitos/metabolismo , Receptores de Hidrocarburo de Aril/fisiología , Piel/efectos de los fármacos , Piel/metabolismo
3.
World J Microbiol Biotechnol ; 38(12): 222, 2022 Sep 14.
Artículo en Inglés | MEDLINE | ID: mdl-36100774

RESUMEN

Intestinal peristalsis is essential for gastrointestinal function, which could maintain the appropriate progression and digestion of food and reduce bacterial aggregation through mixing function. Even though certain ingredients of foodstuff are known to increase or decrease intestinal peristalsis, the role of environmental pollutants on intestinal peristalsis is relatively unknown. Therefore, the effects of four typical environmental pollutants (oxytetracycline, arsenic, polychlorinated biphenyls and chlorpyrifos) on intestinal peristalsis in the zebrafish model and then tested the recovery effect of the constipation-resistant probiotic. The results showed that 4-day environmental pollutants exposures on the zebrafish embryos at 1 day post fertilization clearly decreased the intestinal peristalsis through decreasing the serotonin (5-HT) production and down-regulating the expression of key genes involved in 5-HT synthesis. Pollutants-evoked change of gut motility could be normalized in the presence of Lactobacillus rhamnosus GG (LGG) via increasing 5-HT secretion. Exogenous 5-hydroxytryptophan (100 µg/L) could also rescue the dysfunction of gut motility in pollutants-treated zebrfish. The data identified that LGG normalized disorder of intestinal peristalsis induced by environmental pollutants through increasing 5-HT level. The stimulant effect of LGG on peristalsis may be associated with 5-HT system, which could provide references for the application of probiotics in regulation of gut dysmotility.


Asunto(s)
Contaminantes Ambientales , Lactobacillus rhamnosus , Animales , Contaminantes Ambientales/metabolismo , Contaminantes Ambientales/toxicidad , Lactobacillus rhamnosus/genética , Lactobacillus rhamnosus/metabolismo , Larva , Serotonina/metabolismo , Pez Cebra
4.
Metabolomics ; 18(9): 73, 2022 Sep 09.
Artículo en Inglés | MEDLINE | ID: mdl-36083566

RESUMEN

INTRODUCTION: Work-related exposures to harmful agents or factors are associated with an increase in incidence of occupational diseases. These exposures often represent a complex mixture of different stressors, challenging the ability to delineate the mechanisms and risk factors underlying exposure-disease relationships. The use of omics measurement approaches that enable characterization of biological marker patterns provide internal indicators of molecular alterations, which could be used to identify bioeffects following exposure to a toxicant. Metabolomics is the comprehensive analysis of small molecule present in biological samples, and allows identification of potential modes of action and altered pathways by systematic measurement of metabolites. OBJECTIVES: The aim of this study is to review the application of metabolomics studies for use in occupational health, with a focus on applying metabolomics for exposure monitoring and its relationship to occupational diseases. METHODS: PubMed, Web of Science, Embase and Scopus electronic databases were systematically searched for relevant studies published up to 2021. RESULTS: Most of reviewed studies included worker populations exposed to heavy metals such as As, Cd, Pb, Cr, Ni, Mn and organic compounds such as tetrachlorodibenzo-p-dioxin, trichloroethylene, polyfluoroalkyl, acrylamide, polyvinyl chloride. Occupational exposures were associated with changes in metabolites and pathways, and provided novel insight into the relationship between exposure and disease outcomes. The reviewed studies demonstrate that metabolomics provides a powerful ability to identify metabolic phenotypes and bioeffect of occupational exposures. CONCLUSION: Continued application to worker populations has the potential to enable characterization of thousands of chemical signals in biological samples, which could lead to discovery of new biomarkers of exposure for chemicals, identify possible toxicological mechanisms, and improved understanding of biological effects increasing disease risk associated with occupational exposure.


Asunto(s)
Contaminantes Ambientales , Enfermedades Profesionales , Exposición Profesional , Biomarcadores , Contaminantes Ambientales/análisis , Contaminantes Ambientales/toxicidad , Humanos , Metabolómica , Enfermedades Profesionales/etiología , Exposición Profesional/efectos adversos , Exposición Profesional/análisis
6.
Ecotoxicol Environ Saf ; 242: 113933, 2022 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-35930840

RESUMEN

In the more than 100 years since the invention of plastics, various plastic polymers have been developed that exhibit different characteristics and have been widely used in production and life. In 2020 alone, nearly 400 million tons of plastics were produced globally. However, while plastic such as polyethylene brings us convenience, it also threatens environmental sustainability and human health. Due to insufficient recycling efficiency, millions of tons of polyethylene pollutants accumulate in terrestrial or marine environments each year. Polyethylene is elastic, chemically stable, and non-biodegradable, and the traditional disposal methods include landfilling and incineration. These methods are costly, unsustainable, and further increase the burden on the environment. Therefore, recent research has increasingly focused on the biodegradation of polyethylene. In this work, we briefly summarized polyethylene's properties and environmental toxicity. We also reviewed the recent advances in the biodegradation of polyethylene with a summary of traditional abiotic methods. Finally, we proposed a brief research direction in polyethylene study with the aspect of environmental toxicology and industrial applications of decomposition technology.


Asunto(s)
Contaminantes Ambientales , Polietileno , Biodegradación Ambiental , Contaminantes Ambientales/química , Contaminantes Ambientales/toxicidad , Sustancias Peligrosas , Humanos , Plásticos/química , Polietileno/metabolismo , Polietileno/toxicidad , Reciclaje
7.
Ying Yong Sheng Tai Xue Bao ; 33(8): 2297-2304, 2022 Aug.
Artículo en Chino | MEDLINE | ID: mdl-36043839

RESUMEN

Oil and its pollutants, which enter environment through natural oil seepage and many human activities, have considerable impacts on birds. We summarized the research advances in how oil pollutants influence birds and the cleaning technology of polluted birds and their habitats. The toxicity and destruction to feather structure are the major impacts of oil pollution on birds. Oil pollution can lead to birds' death, and also produce many chronic harms, including causing hemolytic anemia, reducing their immunity, disrupting thermal insulation and waterproo-fing performance of feather. It is an important way to reduce the impacts of oil pollution on birds by timely cleaning up the oil in bird habitats as well as carrying out the clean and repair work to the polluted birds. As a big oil-consuming country, China has been left behind by foreign countries in the studies of the effects of oil pollution on birds. More attention should be paid on the short-term and long-term impacts of oil pollution on birds and the cleaning and remediation technologies of the polluted birds and their habitats.


Asunto(s)
Contaminantes Ambientales , Contaminación por Petróleo , Contaminantes Químicos del Agua , Animales , Aves , Ecosistema , Contaminantes Ambientales/toxicidad , Humanos , Contaminantes Químicos del Agua/análisis
8.
Environ Res ; 214(Pt 3): 114110, 2022 11.
Artículo en Inglés | MEDLINE | ID: mdl-35985486

RESUMEN

The ever-increasing number of chemicals and complex mixtures demands a time-saving and cost-effective platform for environmental risk assessment. However, there is limit promising tool for evaluating the contribution of each component to the total toxicity effects of the mixture. Here, four widely distributed environmental pollutants with different mode-of-actions, i.e., cadmium chloride (Cd), nitrofurazone (NFZ), triclosan (TCS), and tris(2-chloroethyl) phosphate (TCEP), were selected as components of artificial mixture. Integration of leave-one-out method and high-dimensional live cell array system was used to explore relative contribution of each component from the mixture. A quaternary mixture (All_4_chems) and four ternary mixtures (Leave_Cd, Leave_NFZ, Leave_TCS and Leave_TCEP) were investigated by Escherichia coli (E. coli) live cell array system with 90 environmental stress genes modified by green fluorescent protein (GFP) expressing reporter vectors. E. coli cytotoxicity tests demonstrated that TCS has antagonism effect with other three chemicals (Cd, NFZ and TCEP), while it was additive effect in other three binary combinations. A total of 26, 23, 13, 31 and 23 genes were significantly altered with fold-change greater than 2 over the 4 h exposure by All_4_chems, Leave_Cd, Leave_NFZ, Leave_TCS and Leave_TCEP, respectively. Clustering analysis based on time-series gene expression patterns and transcriptional effect level index (TELI) showed that Leave_TCEP has similar profiles with All_4_chems, demonstrating TCEP has the least contribution among four components to the quaternary mixture. Leave_NFZ has the least number of significantly altered genes, implying NFZ has the largest toxicity effect contribution to the quaternary mixture. The relative contribution in different pathways indicated that Cd has the most contribution to the mixture in redox stress, while TCS has the least contribution in DNA stress pathway. Collectively, our results demonstrated the utility of high-dimensional toxicogenomics data and leave-one-out method in prioritizing the relative contribution of each component in mixture.


Asunto(s)
Contaminantes Ambientales , Triclosán , Cadmio , Contaminantes Ambientales/toxicidad , Escherichia coli/genética
9.
Environ Res ; 214(Pt 4): 114115, 2022 11.
Artículo en Inglés | MEDLINE | ID: mdl-35988832

RESUMEN

INTRODUCTION: We have recently shown that sperm epigenetic age (SEA), a surrogate measure of biological aging in sperm, is associated with couples' time-to-pregnancy (TTP). Advanced SEA was also observed among smokers, suggesting its susceptibility to environmental exposures. Therefore, we assessed the association between urinary phthalate metabolites and SEA in male partners of couples planning to conceive among the general population. METHOD: The Longitudinal Investigation of Fertility and the Environment (LIFE) Study was a prospective multi-site and general population cohort study of couples who were interested in becoming pregnant. Among male partners (n = 333), eleven urinary phthalate metabolites were measured and SEA was previously developed using Super Learner ensemble algorithm. Multivariable linear regression was used to evaluate associations of SEA with individual metabolites. Bayesian kernel machine regression (BKMR), quantile g-computation (qgcomp) and weighted quantile sum (WQS) models were used for mixture analyses. Covariates included were BMI, cotinine, race and urinary creatinine. RESULT: In the single metabolite multivariate analyses, nine (82%) phthalate metabolites displayed positive trends with SEA (range: 0.05-0.47 years). Of these metabolites, advanced SEA was significantly associated with interquartile range increases in exposure of three phthalates [MEHHP (ß = 0.23, 95% CI: 0.03, 0.43, p = 0.03), MMP (ß = 0.24, 95% CI: 0.01, 0.47, p = 0.04), and MiBP (ß = 0.47, 95% CI: 0.14, 0.81, p = 0.01)]. Additionally, in BKMR and qgcomp (p = 0.06), but not WQS models, phthalate mixtures showed an overall positive trend with SEA, with MiBP, MMP and MBzP as major drivers of the mixture effects. CONCLUSION: This is the first study that combined single exposure and mixture models to associate male phthalate exposures with advanced epigenetic aging of sperm in men planning to conceive among the general population. Our findings suggest that phthalate exposure may contribute to the acceleration of biological aging of sperm.


Asunto(s)
Contaminantes Ambientales , Ácidos Ftálicos , Envejecimiento , Teorema de Bayes , Estudios de Cohortes , Exposición a Riesgos Ambientales , Contaminantes Ambientales/toxicidad , Contaminantes Ambientales/orina , Epigénesis Genética , Femenino , Humanos , Masculino , Ácidos Ftálicos/orina , Embarazo , Estudios Prospectivos , Semen , Espermatozoides
10.
Toxicol Sci ; 189(2): 155-174, 2022 Sep 24.
Artículo en Inglés | MEDLINE | ID: mdl-35951756

RESUMEN

Lipophilic persistent environmental chemicals (LPECs) can accumulate in a woman's body and transfer to her developing child across the placenta and via breast milk. To assess health risks associated with developmental exposures to LPECs, we developed a pharmacokinetic (PK) model that quantifies mother-to-offspring transfer of LPECs during pregnancy and lactation and facilitates internal dosimetry calculations for offspring. We parameterized the model for mice, rats, and humans using time-varying functions for body mass and milk consumption rates. The only required substance-specific parameter is the elimination half-life of the LPEC in the animal species of interest. We used the model to estimate whole-body concentrations in mothers and offspring following maternal exposures to hexachlorobenzene (HCB) and 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153) and compared these with measured concentrations from animal studies. We also compared estimated concentrations for humans to those generated using a previously published human LPEC PK model. Finally, we compared human equivalent doses (HEDs) calculated using our model and an allometric scaling method. Estimated and observed whole-body concentrations of HCB and PCB 153 in offspring followed similar trends and differed by less than 60%. Simulations of human exposure yielded concentration estimates comparable to those generated using the previously published model, with concentrations in offspring differing by less than 12%. HEDs calculated using our PK model were about 2 orders of magnitude lower than those generated using allometric scaling. Our PK model can be used to calculate internal dose metrics for offspring and corresponding HEDs and thus informs assessment of developmental toxicity risks associated with LPECs.


Asunto(s)
Contaminantes Ambientales , Hexaclorobenceno , Animales , Niño , Contaminantes Ambientales/farmacocinética , Contaminantes Ambientales/toxicidad , Femenino , Hexaclorobenceno/toxicidad , Humanos , Lactancia , Ratones , Leche Humana/química , Modelos Biológicos , Madres , Bifenilos Policlorados , Embarazo , Ratas
11.
Sci Total Environ ; 849: 157951, 2022 Nov 25.
Artículo en Inglés | MEDLINE | ID: mdl-35961392

RESUMEN

Microplastics (MPs), an emerging pollutant, are of global concern due to their wide distribution and large quantities. In addition to MPs themselves, various additives within MPs (such as plasticizers, flame retardants, antioxidants and heavy metals) may also have harmful effects on the environment. Most of these additives are physically bound to plastics and can therefore be leached from the plastic and released into the environment. Aging of MPs in the actual environment can affect the migration and release of additives, further increasing the ecotoxicological risk of additives to organisms. This work reviews the functions of several commonly used additives in MPs, and summarizes the representative characterization methods. Furthermore, the migration and leaching of additives in the human environment and marine environment are outlined. As aging promotes the internal chain breaking of MPs and the increase of specific surface area, it in turn stimulates the release of additives. The hazards of additive exposure have been elucidated, and various studies from the laboratory have shown that more toxic additives such as phthalates and brominated flame retardants can disrupt a variety of biological processes in organisms, including metabolism, skeletal development and so on. Increase of MPs ecological risk caused by the leaching of toxic additives is discussed, especially under the effect of aging. This study presents a systematic summary of various functional and environmental behaviors of additives in plastics, using weathering forces as the main factor, which helps to better assess the environmental impact and potential risks of MPs.


Asunto(s)
Contaminantes Ambientales , Retardadores de Llama , Metales Pesados , Contaminantes Químicos del Agua , Envejecimiento , Antioxidantes , Contaminantes Ambientales/toxicidad , Retardadores de Llama/toxicidad , Humanos , Microplásticos , Plastificantes/toxicidad , Plásticos/toxicidad , Contaminantes Químicos del Agua/análisis
12.
Chem Res Toxicol ; 35(8): 1312-1333, 2022 08 15.
Artículo en Inglés | MEDLINE | ID: mdl-35921496

RESUMEN

Per- and polyfluoroalkyl substances (PFAS) are a group of persistent environmental pollutants that are ubiquitously found in the environment and virtually in all living organisms, including humans. PFAS cross the blood-brain barrier and accumulate in the brain. Thus, PFAS are a likely risk for neurotoxicity. Studies that measured PFAS levels in the brains of humans, polar bears, and rats have demonstrated that some areas of the brain accumulate greater amounts of PFAS. Moreover, in humans, there is evidence that PFAS exposure is associated with attention-deficit/hyperactivity disorder (ADHD) in children and an increased cause of death from Parkinson's disease and Alzheimer's disease in elderly populations. Given possible links to neurological disease, critical analyses of possible mechanisms of neurotoxic action are necessary to advance the field. This paper critically reviews studies that investigated potential mechanistic causes for neurotoxicity including (1) a change in neurotransmitter levels, (2) dysfunction of synaptic calcium homeostasis, and (3) alteration of synaptic and neuronal protein expression and function. We found growing evidence that PFAS exposure causes neurotoxicity through the disruption of neurotransmission, particularly the dopamine and glutamate systems, which are implicated in age-related psychiatric illnesses and neurodegenerative diseases. Evaluated research has shown there are highly reproduced increased glutamate levels in the hippocampus and catecholamine levels in the hypothalamus and decreased dopamine in the whole brain after PFAS exposure. There are significant gaps in the literature relative to the assessment of the nigrostriatal system (striatum and ventral midbrain) among other regions associated with PFAS-associated neurologic dysfunction observed in humans. In conclusion, evidence suggests that PFAS may be neurotoxic and associated with chronic and age-related psychiatric illnesses and neurodegenerative diseases. Thus, it is imperative that future mechanistic studies assess the impact of PFAS and PFAS mixtures on the mechanism of neurotransmission and the consequential functional effects.


Asunto(s)
Contaminantes Ambientales , Fluorocarburos , Síndromes de Neurotoxicidad , Anciano , Animales , Niño , Dopamina/metabolismo , Contaminantes Ambientales/metabolismo , Contaminantes Ambientales/toxicidad , Fluorocarburos/toxicidad , Glutamatos , Humanos , Síndromes de Neurotoxicidad/metabolismo , Ratas , Transmisión Sináptica
13.
Ecotoxicol Environ Saf ; 242: 113930, 2022 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-35914397

RESUMEN

PURPOSE: This study aimed to investigate the relationship between phthalate metabolites and renal function. METHODS: We analyzed data from 9989 participants who took part in the National Health and Nutrition Examination Survey (NHANES) from 2005 to 2018. Renal function was reflected by estimated glomerular filtration rate (eGFR), urinary albumin-to-creatinine ratio (UACR), and hypertension. We used generalized linear regression to estimate the correlation between covariate-adjusted creatinine-normalized phthalate metabolites and renal function. In addition, subgroup analysis was used to further compare the effect differences between various populations. RESULTS: In the adjusted model, we found differential associations between phthalates and plasticizers metabolites and renal function. We found that Mono-benzyl phthalate, Mono-(3-carboxypropyl) phthalate, and Mono-(2-ethyl-5-oxohexyl) phthalate were positively associated with lower eGFR with odds ratios (95% confidence intervals) of 1.38 (1.14, 1.67), 1.30 (1.09, 1.57), and 1.27 (1.04, 1.53). While Mono-ethyl phthalate, Mono-(2-ethyl)-hexyl phthalate, Mono-isononyl phthalate and Mono-isobutyl phthalate were negatively associated with lower eGFR with OR values of 0.79 (0.69, 0.90), 0.64 (0.52, 0.78), 0.65 (0.51, 0.82) and 0.80 (0.63, 1.00), respectively. In addition, we found that Mono(carboxyoctyl) phthalate and Mono-isobutyl phthalate were negatively associated with hypertension with ORs of 0.86 (0.78, 0.96) and 0.84 (0.72, 0.98). But phthalates and plasticizers metabolites were not associated with UACR. CONCLUSION: This study found differences in the effects of phthalates and plasticizers metabolites on kidney function, which may raise concerns about possible changes in kidney function resulting from exposure to current levels of plasticizers.


Asunto(s)
Contaminantes Ambientales , Hipertensión , Ácidos Ftálicos , Adulto , Creatinina , Exposición a Riesgos Ambientales , Contaminantes Ambientales/toxicidad , Contaminantes Ambientales/orina , Humanos , Riñón/metabolismo , Encuestas Nutricionales , Ácidos Ftálicos/orina , Plastificantes/toxicidad
14.
Environ Pollut ; 311: 119927, 2022 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-35970344

RESUMEN

Environmental pollutants such as heavy metals, nano/microparticles, and organic compounds have been detected in a wide range of environmental media, causing long-term exposure in various organisms and even humans through breathing, contacting, ingestion, and other routes. Long-term exposure to environmental pollutants in organisms or humans promotes exposure of offspring to parental and environmental pollutants, and subsequently results in multiple biological defects in the offspring. This review dialectically summarizes and discusses the existing studies using Caenorhabditis elegans (C. elegans) as a model organism to explore the multi/transgenerational toxicity and potential underlying molecular mechanisms induced by environmental pollutants following parental or successive exposure patterns. Parental and successive exposure to environmental pollutants induces various biological defects in C. elegans across multiple generations, including multi/transgenerational developmental toxicity, neurotoxicity, reproductive toxicity, and metabolic disturbances, which may be transmitted to progeny through reactive oxygen species-induced damage, epigenetic mechanisms, insulin/insulin-like growth factor-1 signaling pathway. This review aims to arouse researchers' interest in the multi/transgenerational toxicity of pollutants and hopes to explore the possible long-term effects of environmental pollutants on organisms and even humans, as well as to provide constructive suggestions for the safety and management of emerging alternatives.


Asunto(s)
Contaminantes Ambientales , Metales Pesados , Animales , Caenorhabditis elegans , Contaminantes Ambientales/toxicidad , Epigénesis Genética , Humanos , Metales Pesados/toxicidad , Reproducción
15.
Environ Pollut ; 311: 119851, 2022 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-35987286

RESUMEN

Polycyclic aromatic hydrocarbons (PAHs) constitute a significant environmental pollution group that reaches toxic levels with anthropogenic activities. The adverse effects of nanoplastics accumulating in ecosystems with the degradation of plastic wastes are also a growing concern. Previous studies have generally focused on the impact of single PAH or plastic fragments exposure on plants. However, it is well recognized that these contaminants co-exist at varying rates in agricultural soil and water resources. Therefore, it is critical to elucidate the phytotoxicity and interaction mechanisms of mixed pollutants. The current study was designed to comparatively investigate the single and combined effects of anthracene (ANT, 100 mg L-1), fluorene (FLU, 100 mg L-1) and polystyrene nanoplastics (PS, 100 mg L-1) contaminations in wheat. Plants exposed to single ANT, FLU and PS treatments demonstrated decline in growth, water content, high stomatal limitations and oxidative damage. The effect of ANT + FLU on these parameters was more detrimental. In addition, ANT and/or FLU treatments significantly suppressed photosynthetic capacity as determined by carbon assimilation rate (A) and chlorophyll a fluorescence transient. The antioxidant system was not fully activated (decreased superoxide dismutase, peroxidase and glutathione reductase) under ANT + FLU, then hydrogen peroxide (H2O2) content (by 2.7-fold) and thiobarbituric acid reactive substances (TBARS) (by 2.8-fold) increased. Interestingly, ANT + PS and FLU + PS improved the growth, water relations and gas exchange parameters. The presence of nanoplastics recovered the adverse effects of ANT and FLU on growth by protecting the photosynthetic photochemistry and reducing oxidative stress. PAH plus PS reduced the ANT and FLU accumulation in wheat leaves. In parallel, the increased antioxidant system, regeneration of ascorbate, glutathione and glutathione redox status observed under ANT + PS and FLU + PS. These findings will provide an information about the phytotoxicity mechanisms of mixed pollutants in the environment.


Asunto(s)
Contaminantes Ambientales , Hidrocarburos Policíclicos Aromáticos , Antioxidantes/metabolismo , Clorofila/metabolismo , Clorofila A , Ecosistema , Contaminantes Ambientales/toxicidad , Fluorescencia , Glutatión , Peróxido de Hidrógeno/metabolismo , Microplásticos , Plásticos , Hidrocarburos Policíclicos Aromáticos/química , Hidrocarburos Policíclicos Aromáticos/toxicidad , Poliestirenos/toxicidad , Triticum/metabolismo , Agua , Abastecimiento de Agua
16.
Toxicology ; 479: 153292, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-35995124

RESUMEN

The genetic variability of toxicant responses among indisviduals in humans and mammalian models requires practically untenable sample sizes to create comprehensive chemical hazard risk evaluations. To address this need, tractable model systems enable reproducible and efficient experimental workflows to collect high-replication measurements of exposure cohorts. Caenorhabditis elegans is a premier toxicology model that has revolutionized our understanding of cellular responses to environmental pollutants and boasts robust genomic resources and high levels of genetic variation across the species. In this study, we performed dose-response analysis across 23 environmental toxicants using eight C. elegans strains representative of species-wide genetic diversity. We observed substantial variation in EC10 estimates and slope parameter estimates of dose-response curves of different strains, demonstrating that genetic background is a significant driver of differential toxicant susceptibility. We also showed that, across all toxicants, at least one C. elegans strain exhibited a significantly different EC10 or slope estimate compared to the reference strain, N2 (PD1074), indicating that population-wide differences among strains are necessary to understand responses to toxicants. Moreover, we quantified the heritability of responses (phenotypic variance attributable to genetic differences between individuals) to each toxicant exposure and observed a correlation between the exposure closest to the species-agnostic EC10 estimate and the exposure that exhibited the most heritable response. At least 20% of the variance in susceptibility to at least one exposure level of each compound was explained by genetic differences among the eight C. elegans strains. Taken together, these results provide robust evidence that heritable genetic variation explains differential susceptibility across an array of environmental pollutants and that genetically diverse C. elegans strains should be deployed to aid high-throughput toxicological screening efforts.


Asunto(s)
Caenorhabditis elegans , Contaminantes Ambientales , Animales , Caenorhabditis elegans/genética , Contaminantes Ambientales/toxicidad , Genómica , Sustancias Peligrosas , Mamíferos , Modelos Biológicos
17.
J Hazard Mater ; 440: 129768, 2022 10 15.
Artículo en Inglés | MEDLINE | ID: mdl-36027754

RESUMEN

Every two years, the Pollutant Toxic Ions and Molecules Conference, PTIM, meets the environmentalist, biologist, chemists and health researchers in Costa de Caparica, Portugal, to showcase the latest technologies, methodologies and research advances in pollution detection, contamination control, remediation, and related health issues, as well as policy implications.


Asunto(s)
Contaminantes Ambientales , Restauración y Remediación Ambiental , Contaminantes Ambientales/toxicidad , Contaminación Ambiental , Iones , Pandemias
18.
Aquat Toxicol ; 251: 106274, 2022 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-36037606

RESUMEN

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) are widely used and considered as emerging persistent pollutants, posing a potential threat to the aquatic ecosystem due to their metabolic toxicity. However, the effects of early-life PFOA and PFOS exposure on metabolic disruption and underlying mechanisms are not fully understood. Therefore, we investigated the effects of early-life PFOA or PFOS exposure on lipid accumulation, feeding behaviors, fatty acids composition, and possible genetic regulation using the nematode Caenorhabditis elegans as an in vivo model. Our results showed that low concentrations of PFOA and PFOS (0.1 and 1 µM) induced obesity in C. elegans, which was not due to the increased feeding rate. The altered fatty acid composition illustrated the decrease of saturated fatty acids and the increase of polyunsaturated fatty acids. Furthermore, the mutant assay and mRNA levels revealed that fatty acid desaturation related genes mdt-15, nhr-49, fat-6 as well as fatty acid (fasn-1) and triglyceride (TG) (dgat-2) synthesis related genes, were associated with the increased body fat, TG, and lipid droplet (LD) contents in C. elegans exposed to PFOA and PFOS. Hence, this present study provides the genetic regulatory information of PFOA and PFOS induced metabolic disruption of lipid metabolism and obesity.


Asunto(s)
Ácidos Alcanesulfónicos , Contaminantes Ambientales , Fluorocarburos , Contaminantes Químicos del Agua , Ácidos Alcanesulfónicos/toxicidad , Animales , Caenorhabditis elegans/genética , Caprilatos/toxicidad , Ecosistema , Contaminantes Ambientales/toxicidad , Ácidos Grasos , Fluorocarburos/toxicidad , Metabolismo de los Lípidos , Obesidad , ARN Mensajero , Triglicéridos , Contaminantes Químicos del Agua/toxicidad
19.
Neurotoxicology ; 92: 131-155, 2022 09.
Artículo en Inglés | MEDLINE | ID: mdl-35914637

RESUMEN

Investigation of the toxicity triggered by chemicals on the human brain has traditionally relied on approaches using rodent in vivo models and in vitro cell models including primary neuronal cultures and cell lines from rodents. The issues of species differences between humans and rodents, the animal ethical concerns and the time and cost required for neurotoxicity studies on in vivo animal models, do limit the use of animal-based models in neurotoxicology. In this context, human cell models appear relevant in elucidating cellular and molecular impacts of neurotoxicants and facilitating prioritization of in vivo testing. The SH-SY5Y human neuroblastoma cell line (ATCC® CRL-2266™) is one of the most used cell lines in neurosciences, either undifferentiated or differentiated into neuron-like cells. This review presents the characteristics of the SH-SY5Y cell line and proposes the results of a systematic review of literature on the use of this in vitro cell model for neurotoxicity research by focusing on organic environmental pollutants including pesticides, 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD), flame retardants, PFASs, parabens, bisphenols, phthalates, and PAHs. Organic environmental pollutants are widely present in the environment and increasingly known to cause clinical neurotoxic effects during fetal & child development and adulthood. Their effects on cultured SH-SY5Y cells include autophagy, cell death (apoptosis, pyroptosis, necroptosis, or necrosis), increased oxidative stress, mitochondrial dysfunction, disruption of neurotransmitter homeostasis, and alteration of neuritic length. Finally, the inherent advantages and limitations of the SH-SY5Y cell model are discussed in the context of chemical testing.


Asunto(s)
Contaminantes Ambientales , Retardadores de Llama , Fluorocarburos , Neuroblastoma , Síndromes de Neurotoxicidad , Plaguicidas , Dibenzodioxinas Policloradas , Adulto , Animales , Línea Celular Tumoral , Supervivencia Celular , Niño , Contaminantes Ambientales/toxicidad , Retardadores de Llama/farmacología , Fluorocarburos/farmacología , Humanos , Neuroblastoma/metabolismo , Síndromes de Neurotoxicidad/etiología , Parabenos/farmacología , Plaguicidas/farmacología , Dibenzodioxinas Policloradas/farmacología
20.
Food Chem Toxicol ; 168: 113377, 2022 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-35995078

RESUMEN

Per- and polyfluoroalkyl substances (PFAS) are organofluorine compounds has been manufactured for more than five decades and used in different purposes. Among persistent organic pollutants, PFAS are toxic, bioaccumulative in humans, wildlife, and global environment. As per environmental protection agency (EPA) guidelines, the perfluorooctanoate and perfluorooctane sulfonate permissible limit was 0.07 ng/L in drinking water. When the concentration exceeds the acceptable limit, it has negative consequences for humans. In such a case, PFAS monitoring is critical, and a quick detection technique are highly needed. Health departments and regulatory agencies have interests in monitoring of PFAS presences and exposures. For the detection of PFAS, numerous highly precise and sensitive chromatographic methods are available. However, the drawbacks of analytical techniques include timely sample preparations and the lack of on-site applicability. As a result, there is an increasing demand for simple sensor systems for monitoring of PFAS in real field samples. In this review, we first describe the sample pre-treatment and analytical techniques for the detection of PFAS. Second, we broadly discussed available sensor system for the quantification of PFAS in different filed samples. Finally, future trends in PFASs sensor are also presented.


Asunto(s)
Agua Potable , Contaminantes Ambientales , Fluorocarburos , Agua Potable/análisis , Contaminantes Ambientales/análisis , Contaminantes Ambientales/toxicidad , Fluorocarburos/análisis , Fluorocarburos/toxicidad , Humanos , Contaminantes Orgánicos Persistentes , Estados Unidos , United States Environmental Protection Agency
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