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1.
Recurso de Internet en Portugués | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-47009

RESUMEN

Os Coronavírus são uma família de vírus conhecidos por causar doença no ser humano. A infeção pode ser semelhante a uma gripe comum ou apresentar-se como doença mais grave, como pneumonia


Asunto(s)
Infecciones por Coronavirus/transmisión , Coronavirus del Síndrome Respiratorio de Oriente Medio , Betacoronavirus
2.
Recurso de Internet en Portugués | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-47011

RESUMEN

O novo coronavírus, intitulado 2019-nCoV, foi identificado pela primeira vez em janeiro de 2020 na China, na Cidade de Wuhan. Este novo agente nunca tinha sido previamente identificado em seres humanos, tendo causado um surto na cidade de Wuhan. A fonte da infeção é ainda desconhecida.


Asunto(s)
Betacoronavirus , Infecciones por Coronavirus/prevención & control , Coronavirus del Síndrome Respiratorio de Oriente Medio , Infecciones por Coronavirus/transmisión , Virus del SRAS
3.
; OMS.
Recurso de Internet en Inglés, Español, Francés, Arabe, Ruso, Chino | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-47014

RESUMEN

Un nuevo coronavirus (CoV) es una nueva cepa de coronavirus que no ‎se había identificado previamente en el ser humano. El nuevo ‎coronavirus, que ahora se conoce con el nombre de 2019-nCoV, no se ‎había detectado antes de que se notificara el brote en Wuhan (China) ‎en diciembre de 2019.‎


Asunto(s)
Betacoronavirus , Síndrome Respiratorio Agudo Grave/transmisión , Síndrome Respiratorio Agudo Grave/prevención & control , Coronavirus del Síndrome Respiratorio de Oriente Medio
4.
; Brasil.
Recurso de Internet en Portugués | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-47007

RESUMEN

A Organização Mundial da Saúde (OMS) anunciou o nome para a doença causada pelo novo coronavírus: COVID-19. Segundo o diretor-geral da OMS, Tedros Adhanom Ghebreyesus, o ato de nomear é fundamental para prevenir o uso de outros nomes que podem ser imprecisos ou gerar estigma.


Asunto(s)
Coronavirus , Coronavirus del Síndrome Respiratorio de Oriente Medio , Betacoronavirus
5.
Recurso de Internet en Inglés, Español | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-46987

RESUMEN

The Pan American Health Organization has updated an alert to its member countries on the Novel Coronavirus (nCoV), recommending that they strengthen surveillance activities to detect patients with acute respiratory disease, and that “health care workers have access to up to date information on the illness, be familiar with the principles and procedures for handling nCoV infections, and be trained to consult a patient’s travel history to link this information with clinical data.”


Asunto(s)
Virus del SRAS , Coronavirus del Síndrome Respiratorio de Oriente Medio , Control de Enfermedades Transmisibles , Infecciones por Coronavirus
6.
Recurso de Internet en Inglés, Español, Portugués | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-46976

RESUMEN

Em 30 de janeiro de 2020, a OMS declarou que o surto do novo coronavírus (2019-nCoV) constitui uma Emergência de Saúde Pública de Importância Internacional (ESPII).


Asunto(s)
Coronavirus del Síndrome Respiratorio de Oriente Medio , Virus del SRAS , Coronavirus
7.
Recurso de Internet en Portugués | LIS - Localizador de Información en Salud, LIS-bvsms | ID: lis-LISBR1.1-46980

RESUMEN

Em 30 de janeiro de 2020, a OMS declarou que o surto do novo coronavírus (2019-nCoV) constitui uma Emergência de Saúde Pública de Importância Internacional (ESPII).


Asunto(s)
Infecciones por Coronavirus , Coronavirus del Síndrome Respiratorio de Oriente Medio , Betacoronavirus , Virus del SRAS
8.
Medicine (Baltimore) ; 99(3): e18782, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-32011472

RESUMEN

Hemodialysis (HD) patients had a high rate of infection transmission and mortality during the middle east respiratory syndrome coronavirus (MERS-CoV) outbreak in Saudi Arabia. A standardized guideline on isolation technique for exposed HD patients is not available. Thus, this study aimed to evaluate the effect of different isolation strategies on the prevention of secondary viral transmission and clinical outcomes among exposed HD patients.During the 2015 MERS-CoV outbreak in Korea, 116 patients in 3 HD units were incidentally exposed to individuals with confirmed MERS-CoV infection and underwent different types of isolation, which were as follows: single-room isolation (n = 54, 47%), cohort isolation (n = 46, 40%), and self-imposed quarantine (n = 16, 13%). The primary outcome was rate of secondary viral transmission. The secondary outcome measures were changes in clinical and biochemical markers during the isolation period, difference in clinical and biochemical markers according to the types of isolation practice, and effect of isolation practice on patient survival.During a mean isolation period of 15 days, no further cases of secondary transmission were detected among HD patients. Plasma hemoglobin, serum calcium, and serum albumin levels and single-pool Kt/V decreased during the isolation period but normalized thereafter. Patients who were subjected to self-imposed quarantine had higher systolic and diastolic blood pressure, lower total cholesterol level, and lower Kt/V than those who underwent single-room or cohort isolation. During the 24-month follow-up period, 12 patients died. However, none of the deaths occurred during the isolation period, and no differences were observed in patient survival rate according to different isolation strategies.Although 116 participants in 3 HD units were incidentally exposed to MERS-CoV during the 2015 outbreak in Korea, strict patient surveillance and proper isolation practice prevented secondary transmission of the virus. Thus, a renal disaster protocol, which includes proper contact surveillance and isolation practice, must be established in the future to accommodate the needs of HD patients during disasters or outbreaks.


Asunto(s)
Infecciones por Coronavirus/prevención & control , Infección Hospitalaria/prevención & control , Coronavirus del Síndrome Respiratorio de Oriente Medio , Aislamiento de Pacientes , Diálisis Renal , Anciano , Infecciones por Coronavirus/sangre , Infecciones por Coronavirus/mortalidad , Infecciones por Coronavirus/transmisión , Infección Hospitalaria/sangre , Infección Hospitalaria/mortalidad , Infección Hospitalaria/transmisión , Femenino , Humanos , Fallo Renal Crónico/terapia , Masculino , Persona de Mediana Edad , Aislamiento de Pacientes/métodos , Estudios Prospectivos , Cuarentena , Resultado del Tratamiento
9.
Recurso de Internet en Inglés, Español, Francés, Arabe, Ruso, Chino | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-46952

RESUMEN

Coronaviruses (CoV) are a large family of viruses that cause illness ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). A novel coronavirus (nCoV) is a new strain that has not been previously identified in humans.


Asunto(s)
Infecciones por Coronavirus , Coronavirus del Síndrome Respiratorio de Oriente Medio , Síndrome Respiratorio Agudo Grave
10.
Recurso de Internet en Portugués | LIS - Localizador de Información en Salud | ID: lis-LISBR1.1-46945

RESUMEN

Site do Ministério da Saúde. (Brasil). Tirando dúvidas sobre o corona Virús. O que é o Virús, suas causas, sintomas, tratamento, diagnostico é prevenção Saiba mais sobre o Coronavirus...


Asunto(s)
Coronavirus del Síndrome Respiratorio de Oriente Medio/patogenicidad , Coronavirus Humano 229E/patogenicidad , Infecciones por Coronavirus/prevención & control
12.
BMC Infect Dis ; 19(1): 870, 2019 Oct 22.
Artículo en Inglés | MEDLINE | ID: mdl-31640578

RESUMEN

BACKGROUND: Mortality is high among patients with Middle East Respiratory Syndrome Coronavirus (MERS-CoV) infection. We aimed to determine hospital mortality and the factors associated with it in a cohort of MERS-CoV patients. METHODS: We reviewed hospital records of confirmed cases (detection of virus by polymerase chain reaction from respiratory tract samples) of MERS-CoV patients (n = 63) admitted to Buraidah Central Hospital in Al-Qassim, Saudi Arabia between 2014 and 2017. We abstracted data on demography, vital signs, associated conditions presented on admission, pre-existing chronic diseases, treatment, and vital status. Bi-variate comparisons and multiple logistic regressions were the choice of data analyses. RESULTS: The mean age was 60 years (SD = 18.2); most patients were male (74.6%) and Saudi citizens (81%). All but two patients were treated with Ribavirin plus Interferon. Hospital mortality was 25.4%. Patients who were admitted with septic shock and/or organ failure were significantly more likely to die than patients who were admitted with pneumonia and/or acute respiratory distress syndrome (OR = 47.9, 95% CI = 3.9, 585.5, p-value 0.002). Age, sex, and presence of chronic conditions were not significantly associated with mortality. CONCLUSION: Hospital mortality was 25%; septic shock/organ failure at admittance was a significant predictor of mortality.


Asunto(s)
Infecciones por Coronavirus/tratamiento farmacológico , Infecciones por Coronavirus/mortalidad , Mortalidad Hospitalaria , Adulto , Anciano , Antivirales/uso terapéutico , Estudios de Cohortes , Infecciones por Coronavirus/complicaciones , Femenino , Humanos , Pacientes Internos/estadística & datos numéricos , Interferón-alfa/uso terapéutico , Modelos Logísticos , Masculino , Persona de Mediana Edad , Coronavirus del Síndrome Respiratorio de Oriente Medio/genética , Neumonía/tratamiento farmacológico , Neumonía Viral/tratamiento farmacológico , Neumonía Viral/etiología , Neumonía Viral/mortalidad , Reacción en Cadena de la Polimerasa , Ribavirina/uso terapéutico , Arabia Saudita/epidemiología , Resultado del Tratamiento
13.
Emerg Microbes Infect ; 8(1): 1528-1534, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31645223

RESUMEN

Dromedary camels are important reservoir hosts of various coronaviruses, including Middle East respiratory syndrome coronavirus (MERS-CoV) that cause human infections. CoV genomes regularly undergo recombination during infection as observed in bat SARS-related CoVs. Here we report for the first time that only a small proportion of MERS-CoV receptor-binding domain positive (RBD) of spike protein positive camel sera in Kenya were also seropositive to MERS-CoV nucleocapsid (NP). In contrast, many of them contain antibodies against bat HKU8-related (HKU8r)-CoVs. Among 584 camel samples that were positive against MERS-CoV RBD, we found only 0.48 (8.22%) samples were also positive for NP. Furthermore, we found bat HKU8r-CoV NP antibody in 73 (12.5%) of the MERS-CoV RBD positive and NP negative samples, yet found only 3 (0.43%) of the HKU8r-CoV S1 antibody in the same samples. These findings may indicate co-infection with MERS-CoV and a HKU8r-CoV in camels. It may also raise the possibility of the circulation of a recombinant coronavirus virus with the spike of MERS-CoV and the NP of a HKU8r-CoV in Kenya. We failed to find molecular evidence of an HKU8r-CoV or a putative recombinant virus. Our findings should alert other investigators to look for molecular evidence of HKU8r-CoV or recombinants.


Asunto(s)
Camelus/virología , Infecciones por Coronavirus/veterinaria , Coronavirus/inmunología , Coronavirus del Síndrome Respiratorio de Oriente Medio/inmunología , Animales , Anticuerpos Antivirales/sangre , Camelus/sangre , Quirópteros/virología , Coronavirus/genética , Coronavirus/aislamiento & purificación , Infecciones por Coronavirus/sangre , Infecciones por Coronavirus/virología , Kenia , Coronavirus del Síndrome Respiratorio de Oriente Medio/genética , Coronavirus del Síndrome Respiratorio de Oriente Medio/aislamiento & purificación , Proteínas de la Nucleocápside/inmunología , Recombinación Genética , Glicoproteína de la Espiga del Coronavirus/inmunología
14.
Rev Sci Tech ; 38(1): 61-69, 2019 May.
Artículo en Inglés | MEDLINE | ID: mdl-31564740

RESUMEN

Middle East respiratory syndrome (MERS) is a zoonotic viral disease identified in both animals and human beings. More than 2,200 laboratory-confirmed cases have been reported in humans from 27 countries, with a crude case fatality rate of 35% since the disease's emergence in the Middle East in 2012. In the coming years, MERS will continue to pose a severe threat to economic development as well as to the elimination of poverty and advances in food security. An important gap in the effort to keep MERS at bay is the lack of surveillance of animals in the Middle East. The authors identify the need for international collaboration to conduct MERS coronavirus (CoV) surveillance in animals in the Middle East, since the emergence of new MERS-CoV variants with the ability to sustain efficient person-to-person transmission is a genuine threat. However, effective surveillance will be very difficult, if not impossible, to achieve. There are multiple obstacles in the region to overcome, including a lack of transparency as governments in the Middle East generally do not disclose detailed information on animal diseases. In addition, there is minimal collaboration between local and international agencies in both the human and animal health sectors and a limited number of readily available qualified laboratories to screen animals for MERS- CoV. Last, but not least, there is a lack of adequate active communication between all relevant laboratories, local and abroad. However, with the support of the Food and Agriculture Organization of the United Nations (FAO), the World Organisation for Animal Health (OIE), and other partners, the responsibility of the Mediterranean Zoonosis Control Centre in Athens, Greece, could be widened to include the countries of the Middle East. This would foster a stronger alliance and far more effective collaboration in the spirit of One Health.


Asunto(s)
Infecciones por Coronavirus , Coronavirus del Síndrome Respiratorio de Oriente Medio , Animales , Infecciones por Coronavirus/prevención & control , Humanos , Colaboración Intersectorial , Medio Oriente , Vigilancia de la Población , Zoonosis/prevención & control
15.
J Microbiol Biotechnol ; 29(8): 1316-1323, 2019 Aug 28.
Artículo en Inglés | MEDLINE | ID: mdl-31434175

RESUMEN

Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerging coronavirus which is zoonotic from bats and camels. Its infection in humans can be fatal especially in patients with preexisting conditions due to smoking and chronic obstructive pulmonary disease (COPD). Among the 25 proteins encoded by MERS-CoV, 5 accessory proteins seem to be involved in viral evasion of the host immune responses. Here we report that ORF4a, ORF4b, and ORF8b proteins, alone or in combination, effectively antagonize nuclear factor kappa B (NF-κB) activation. Interestingly, the inhibition of NF-κB by MERS-CoV accessory proteins was mostly at the level of pattern recognition receptors: melanoma differentiationassociated gene 5 (MDA5). ORF4a and ORF4b additively inhibit MDA5-mediated activation of NF-κB while that of retinoic acid-inducible gene 1 (RIG-I) is largely not perturbed. Of note, ORF8b was found to be a novel antagonist of MDA5-mediated NF-kκB activation. In addition, ORF8b also strongly inhibits Tank-binding kinase 1 (TBK1)-mediated induction of NF-κB signaling. Taken together, MERS-CoV accessory proteins are involved in viral escape of NF-κB-mediated antiviral immune responses.


Asunto(s)
Helicasa Inducida por Interferón IFIH1/metabolismo , Coronavirus del Síndrome Respiratorio de Oriente Medio/genética , FN-kappa B/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Transducción de Señal , Animales , Infecciones por Coronavirus/inmunología , Células HEK293 , Interacciones Huésped-Patógeno , Humanos , Helicasa Inducida por Interferón IFIH1/genética , Proteínas Serina-Treonina Quinasas/genética , Proteínas Virales/genética
16.
Nat Biotechnol ; 37(10): 1163-1173, 2019 10.
Artículo en Inglés | MEDLINE | ID: mdl-31451733

RESUMEN

A major limitation of current humanized mouse models is that they primarily enable the analysis of human-specific pathogens that infect hematopoietic cells. However, most human pathogens target other cell types, including epithelial, endothelial and mesenchymal cells. Here, we show that implantation of human lung tissue, which contains up to 40 cell types, including nonhematopoietic cells, into immunodeficient mice (lung-only mice) resulted in the development of a highly vascularized lung implant. We demonstrate that emerging and clinically relevant human pathogens such as Middle East respiratory syndrome coronavirus, Zika virus, respiratory syncytial virus and cytomegalovirus replicate in vivo in these lung implants. When incorporated into bone marrow/liver/thymus humanized mice, lung implants are repopulated with autologous human hematopoietic cells. We show robust antigen-specific humoral and T-cell responses following cytomegalovirus infection that control virus replication. Lung-only mice and bone marrow/liver/thymus-lung humanized mice substantially increase the number of human pathogens that can be studied in vivo, facilitating the in vivo testing of therapeutics.


Asunto(s)
Infecciones por Coronavirus/virología , Modelos Animales de Enfermedad , Pulmón/fisiología , Infección por el Virus Zika/virología , Animales , Anticuerpos Antivirales , Células Presentadoras de Antígenos , Infecciones por Coronavirus/inmunología , Citocinas/genética , Citocinas/metabolismo , Citomegalovirus/fisiología , Femenino , Regulación de la Expresión Génica , Humanos , Inmunohistoquímica , Masculino , Ratones , Ratones SCID , Coronavirus del Síndrome Respiratorio de Oriente Medio/inmunología , Tropismo/inmunología , Replicación Viral , Virus Zika/inmunología , Infección por el Virus Zika/inmunología
17.
J Microbiol ; 57(9): 803-811, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31452044

RESUMEN

Middle East respiratory syndrome coronavirus (MERS-CoV) is a causative agent of severe-to-fatal pneumonia especially in patients with pre-existing conditions, such as smoking and chronic obstructive pulmonary disease (COPD). MERS-CoV transmission continues to be reported in the Saudi Arabian Peninsula since its discovery in 2012. However, it has rarely been epidemic outside the area except one large outbreak in South Korea in May 2015. The genome of the epidemic MERS-CoV isolated from a Korean patient revealed its homology to previously reported strains. MERS-CoV encodes 5 accessory proteins and generally, they do not participate in the genome transcription and replication but rather are involved in viral evasion of the host innate immune responses. Here we report that ORF8b, an accessory protein of MERS-CoV, strongly inhibits both MDA5- and RIG-I-mediated activation of interferon beta promoter activity while downstream signaling molecules were left largely unaffected. Of note, MDA5 protein levels were significantly down-regulated by ORF8b and co-expression of ORF4a and ORF4b. These novel findings will facilitate elucidation of mechanisms of virus-encoded evasion strategies, thus helping design rationale antiviral countermeasures against deadly MERS-CoV infection.


Asunto(s)
Infecciones por Coronavirus/genética , Interferón beta/genética , Coronavirus del Síndrome Respiratorio de Oriente Medio/metabolismo , Regiones Promotoras Genéticas , Infecciones por Coronavirus/metabolismo , Infecciones por Coronavirus/virología , Proteína 58 DEAD Box/genética , Proteína 58 DEAD Box/metabolismo , Diseño de Drogas , Interacciones Huésped-Patógeno , Humanos , Helicasa Inducida por Interferón IFIH1/genética , Helicasa Inducida por Interferón IFIH1/metabolismo , Interferón beta/metabolismo , Coronavirus del Síndrome Respiratorio de Oriente Medio/genética , Arabia Saudita , Vacunas Virales/genética , Vacunas Virales/inmunología
18.
Nat Commun ; 10(1): 3068, 2019 07 11.
Artículo en Inglés | MEDLINE | ID: mdl-31296843

RESUMEN

Most neutralizing antibodies against Middle East respiratory syndrome coronavirus (MERS-CoV) target the receptor-binding domain (RBD) of the spike glycoprotein and block its binding to the cellular receptor dipeptidyl peptidase 4 (DPP4). The epitopes and mechanisms of mAbs targeting non-RBD regions have not been well characterized yet. Here we report the monoclonal antibody 7D10 that binds to the N-terminal domain (NTD) of the spike glycoprotein and inhibits the cell entry of MERS-CoV with high potency. Structure determination and mutagenesis experiments reveal the epitope and critical residues on the NTD for 7D10 binding and neutralization. Further experiments indicate that the neutralization by 7D10 is not solely dependent on the inhibition of DPP4 binding, but also acts after viral cell attachment, inhibiting the pre-fusion to post-fusion conformational change of the spike. These properties give 7D10 a wide neutralization breadth and help explain its synergistic effects with several RBD-targeting antibodies.


Asunto(s)
Anticuerpos Neutralizantes/inmunología , Anticuerpos Antivirales/inmunología , Infecciones por Coronavirus/inmunología , Coronavirus del Síndrome Respiratorio de Oriente Medio/inmunología , Glicoproteína de la Espiga del Coronavirus/inmunología , Animales , Anticuerpos Neutralizantes/sangre , Anticuerpos Neutralizantes/metabolismo , Anticuerpos Neutralizantes/ultraestructura , Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/metabolismo , Anticuerpos Antivirales/ultraestructura , Línea Celular Tumoral , Infecciones por Coronavirus/sangre , Infecciones por Coronavirus/virología , Cristalografía por Rayos X , Dipeptidil Peptidasa 4/metabolismo , Modelos Animales de Enfermedad , Mapeo Epitopo , Epítopos/inmunología , Femenino , Células HEK293 , Humanos , Ratones , Coronavirus del Síndrome Respiratorio de Oriente Medio/metabolismo , Pruebas de Neutralización , Unión Proteica/inmunología , Dominios Proteicos/inmunología , Proteínas Recombinantes/inmunología , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/ultraestructura , Glicoproteína de la Espiga del Coronavirus/aislamiento & purificación , Glicoproteína de la Espiga del Coronavirus/metabolismo , Glicoproteína de la Espiga del Coronavirus/ultraestructura , Células Vero , Internalización del Virus
20.
Am J Public Health ; 109(9): 1288-1293, 2019 09.
Artículo en Inglés | MEDLINE | ID: mdl-31318592

RESUMEN

Objectives. To explore complex associations among demographic factors, risk factors, health care, and fatality rates of Middle East respiratory syndrome coronavirus (MERS-CoV) in the Kingdom of Saudi Arabia.Methods. We based this study on analysis of a publicly accessible line listing of 1256 MERS-CoV cases (2013 to October 2018) available on the World Health Organization's Web site. For analyses of demographic factors (e.g., age, gender), access to health care, promptness of laboratory services, risk factors (comorbidity, exposure to camels and persons with MERS-CoV), occupation (health care), and outcome (fatality), we used descriptive statistics, risk ratio (RR), and the Pearson χ2 test.Results. Presence of comorbidity (RR = 3; 95% confidence interval [CI] = 2.2, 3.9), being male (RR = 1.6; 95% CI = 1.2, 2.1), exposure to dromedary camels (RR = 1.6; 95% CI = 1.3, 2.3), and consumption of camel milk (RR = 1.5; 95% CI = 0.9, 1.7) can significantly increase risk for fatality. Health care workers have significantly lower fatality (P < .001) than the rest of the persons with MERS-CoV.Conclusions. Policies that promote health awareness for the high-risk population and their prompt seeking of health care should be considered. Publicly accessible line lists of infectious diseases such as MERS-CoV can be valuable sources for epidemiological analysis.


Asunto(s)
Infecciones por Coronavirus/epidemiología , Coronavirus del Síndrome Respiratorio de Oriente Medio , Comorbilidad , Estudios Transversales , Femenino , Humanos , Masculino , Factores de Riesgo , Arabia Saudita/epidemiología
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