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1.
Food Chem ; 335: 127331, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32739802

RESUMEN

Teff is currently being incorporated into a range of foodstuffs, especially gluten-free flakes and extruded products. The main objective of this work was to assess the effects of different treatments (industrial milling, formation of flakes and extrusion) on the phenolic composition of two types of teff grains differing in their colour (white and brown). Fifty-nine phenolic compounds were detected by HPLC-DAD-MSn. C-glycosyl flavones accounted for more than 90% of the total phenolic contents in both teff types. White teff mostly contained apigenin-derived flavones (86-92%), whereas luteolin derivatives prevailed in brown teff (91-94%). The industrial processes, mainly flaking and extrusion, caused marked changes in the phenolic composition, some of which were dependent on the teff type. In both teff types, processing changed the phenolic profiles similarly by increasing C-monoglycosyl flavones and decreasing acylated derivatives. However, the total content was increased in flakes and extruded products made only from brown teff grains.


Asunto(s)
Eragrostis/química , Manipulación de Alimentos , Industrias , Fenoles/química , Cromatografía Líquida de Alta Presión , Fenoles/análisis
2.
Food Chem ; 336: 127730, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32768900

RESUMEN

Phenols are responsible for the only health claim of virgin olive oil (VOO) recognized by the European Commission EU 432/2012 and the European Food Safety Authority. In this research, we studied the decrease in the phenolic content of 160 extra VOOs (EVOOs) after 12 months storage in darkness at 20 °C. Phenolic concentration was decreased 42.0 ± 24.3% after this period and this reduction strongly depended on the initial phenolic profile. Hence, EVOOs with predominance in oleacein and oleocanthal experienced a larger decrease in phenolic content than oils enriched in other phenols. Complementarily, hydroxytyrosol and oleocanthalic acid increased significantly in aged EVOOs, which allowed their discrimination from recently produced EVOOs. These changes are explained by degradation of main secoiridoids during storage due to their antioxidant properties. Hydroxytyrosol and oleocanthalic acid can be considered markers of olive oil ageing, although they can also provide information about quality or stability.


Asunto(s)
Almacenamiento de Alimentos/métodos , Aceite de Oliva/química , Fenoles/química , Antioxidantes/química , Área Bajo la Curva , Cromatografía Líquida de Alta Presión , Iridoides/análisis , Fenoles/análisis , Alcohol Feniletílico/análogos & derivados , Alcohol Feniletílico/análisis , Curva ROC , Espectrometría de Masas en Tándem , Factores de Tiempo
3.
Food Chem ; 336: 127722, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32771902

RESUMEN

In this article we describe a new and simple analytical method based on the Quick, Easy, Cheap, Effective, Rugged and Safe technique followed by dispersive solid-phase extraction clean-up with C18 and Lipifiltr® and LC-HRMS for simultaneously extracting six phthalate diesters and six of their metabolites (phthalate monoesters) from highly consumed seafood species. The method was validated for seafood with high and low lipid contents. Apparent recoveries were up to 79% for all compounds. Matrix effect values ranged from -8 to -48% for all compounds in both types of matrices. Method limits of detection were 1-25 ng g-1 dry weight (d.w.) for most compounds. Five seafood species were analysed using this method, and several phthalate diesters and monoesters were successfully quantified. Phthalate diesters were found at concentrations of up to 982 ng g-1 (d.w.) and phthalate monoesters were found at concentrations of up to 178 ng g-1 (d.w.).


Asunto(s)
Espectrometría de Masas/métodos , Ácidos Ftálicos/análisis , Alimentos Marinos/análisis , Cromatografía Líquida de Alta Presión , Ésteres/química , Límite de Detección , Ácidos Ftálicos/aislamiento & purificación , Ácidos Ftálicos/metabolismo , Extracción en Fase Sólida
4.
Food Chem ; 334: 127471, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32688174

RESUMEN

Optimal QuEChERS (quick, easy, cheap, effective, rugged and safe) conditions with good accuracy, repeatability and precision were established to rapidly extract the European Union (EU) priority polycyclic aromatic hydrocarbons (PAHs) from various food matrices (Category: Poultry and Meat, Fish and seafood, Grains, Soy beans and products, Root vegetables and Coffee). The QuEChERS conditions combined with the established high performance liquid chromatography-fluorescence detection conditions were used to rapidly determine the PAHs in 19 popular cooked foods in Taiwan and their corresponding original materials. These conditions also meet the EU and Taiwan Food and Drug Administration specifications. Charcoal grilled, gas stove grilled and smoked foods had higher PAHs contents, while fried and electric oven-baked/baked foods had lower PAHs contents. In addition to the effects of cooking methods, the contamination of original materials by PAHs in the environment should also have an important impact on the contents of PAHs in these cooked foods.


Asunto(s)
Análisis de los Alimentos/métodos , Contaminación de Alimentos/análisis , Hidrocarburos Policíclicos Aromáticos/análisis , Carbón Orgánico , Fraccionamiento Químico/métodos , Cromatografía Líquida de Alta Presión , Culinaria , Unión Europea , Cromatografía de Gases y Espectrometría de Masas/métodos , Límite de Detección , Carne/análisis , Reproducibilidad de los Resultados , Alimentos Marinos/análisis , Taiwán , Verduras/química
5.
Food Chem ; 337: 127752, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-32777573

RESUMEN

The Chinese Yam (Dioscorea opposita) is cultivated widely in China. However, there are potential safety risks associated with herbicide residues in Chinese Yam because of its characteristics and unregulated use of pesticides. We developed an efficient sample preparation technique for simultaneous analysis of atrazine and its degradation products in Chinese Yam using accelerated solvent extraction and pipette tip solid-phase extraction followed by UPLC-DAD analysis. Molecularly imprinted polymer (MIP) were used as the adsorbent material. Method performance was evaluated and our results showed the limit of quantification was 1-3 µg/ kg, with recoveries in the range 79.2-103.8%. When compared with other methods, this method provided superior performance in terms of sensitivity, accuracy, and precision. This method is, therefore, expected to be useful in supervised residue analysis.


Asunto(s)
Atrazina/análisis , Atrazina/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Dioscorea/química , Extracción en Fase Sólida/métodos , Adsorción , Atrazina/química , Impresión Molecular , Polímeros/síntesis química , Polímeros/química , Solventes/química
6.
Food Chem ; 335: 127600, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32736155

RESUMEN

Toosendanin (TSN), as an important Chinese traditional insecticide, has been registered and commercialized in China. In this report, the residual analytical methods, residue dynamics and final residues of TSN in tobacco, cabbage and soil under field condition were studied by IC-ELISA and HPLC. The sensitivity, precision and repeatability of IC-ELISA method were more suitable in comparison with HPLC for the demand of TSN residue analysis. Using IC-ELISA, the half-lives (t1/2) of TSN were found to be 1.30 days in cabbage, 1.70 days in tabacco and 0.71 days in soil, respectively. At the recommended dose, the final residues of TSN detection by IC-ELISA was 0.009 mg·kg-1 in cabbage and 0.043 mg·kg-1 in tobacco, as well as was not detected in soil. Therefore, TSN is easily degradable, and IC-ELISA could be a convenient and supplemental analytical tool for monitoring TSN residue in crops and environment.


Asunto(s)
Brassica/metabolismo , Medicamentos Herbarios Chinos/metabolismo , Ensayo de Inmunoadsorción Enzimática/métodos , Contaminantes del Suelo/metabolismo , Suelo/química , China , Cromatografía Líquida de Alta Presión/métodos , Semivida , Cinética , Residuos de Plaguicidas/análisis , Tabaco
7.
Food Chem ; 335: 127602, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32739807

RESUMEN

Bioactive phenolics primarily contribute to versatile health benefits of pigeon pea. For the first time, an UPLC-QqQ-MS/MS method was developed for the quantitative analysis of eleven bioactive phenolic compounds in pigeon pea natural resources (seeds, leaves, and roots) and in vitro cultures (calli and hairy roots). The proposed method could be achieved within 6 min of running time, and displayed the satisfactory linearity, sensitivity, precision, accuracy, and stability. According to analytical results, the distribution of eleven target compounds in different organs of pigeon pea was clarified. Also, it was surprisingly found that pigeon pea in vitro cultures exhibited superiority in contents of genistin and cajaninstilbene acid as compared with natural resources. Overall, the present work provided a rapid and sensitive analysis approach, which could be useful not only for quality control of pigeon pea natural resources, but also for applicability and safety evaluation of pigeon pea in vitro cultures.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Guisantes/química , Fenoles/análisis , Espectrometría de Masas en Tándem/métodos , Medicamentos Herbarios Chinos/química , Hojas de la Planta/química , Control de Calidad , Reproducibilidad de los Resultados , Semillas/química
8.
Food Chem ; 336: 127539, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32763730

RESUMEN

Hesperidin hydrolysates (HHS) was produced by the hydrolysis of hesperidin (HDN) in previous studies. The potential components in HHS were identified by LC-MS, and minor components (MCS) in HHS were isolated. Antioxidant activities by radical-scavenging capacities, reducing capacity and ß-carotene-linoleate assay, anti-inflammatory effects by inhibiting NO production of RAW 264.7 cells, and α-glucosidase inhibitory effects of HDN, HHS, MCS and henperetin (HTN) were investigated in present study. HHS showed higher radical scavenging activities, higher reducing capacity, and higher inhibitory activity in the ß-carotene-linoleate assay than HDN. HHS inhibited the production of NO and pro-inflammatory cytokines of RAW 264.7 cells more strongly than HDN. HHS also intensively inhibited α-glucosidase activity whereas HDN showed little activity. In addition, the effects of MCS on above activities showed it play a synergistic part with HTN. This work suggested that hydrolyzation of HDN enhance the activities, and provided valuable information on effective utilization of HDN.


Asunto(s)
Antiinflamatorios/química , Antioxidantes/química , Citrus/química , Inhibidores Enzimáticos/química , Hesperidina/análisis , Animales , Antiinflamatorios/farmacología , Catálisis , Supervivencia Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Citrus/metabolismo , Inhibidores Enzimáticos/metabolismo , Hesperidina/metabolismo , Hesperidina/farmacología , Hidrólisis , Macrófagos/citología , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Espectrometría de Masas , Ratones , Óxido Nítrico/metabolismo , Células RAW 264.7 , alfa-Glucosidasas/química , alfa-Glucosidasas/metabolismo
9.
Food Chem ; 336: 127616, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32763733

RESUMEN

This study is an example of apple by-products (AP) recycling through a designed fermentation by selected autochthonous Lactobacillus plantarum AFI5 and Lactobacillus fabifermentans ALI6 used singly or as binary cultures with the selected Saccharomyces cerevisiae AYI7. Compared to Raw-, Unstarted- and Chemically Acidified-AP, Fermented-AP promoted the highest levels of total and insoluble dietary fibers, DPPH scavenging capacity, and free phenolics. The binary culture of L. plantarum AFI5 and S. cerevisiae AYI7 had the best effect on the bioavailability phenolic compounds as resulted by the LC-MS/MS validated method. The accumulation of phenolic acids derivatives highlighted the microbial metabolism during AP fermentation. Bio-converted phenolics were likely responsible for the increased DPPH scavenging capacity. The potential health-promoting effects of Fermented-AP were highlighted using Caco-2 cells. With variations among single and binary cultures, fermented-AP counteracted the inflammatory processes and the effects of oxidative stress in Caco-2 cells, and preserved the integrity of tight junctions.


Asunto(s)
Suplementos Dietéticos/análisis , Malus/química , Fenoles/química , Antiinflamatorios/análisis , Antiinflamatorios/química , Antiinflamatorios/farmacología , Antioxidantes/química , Técnicas de Cultivo Celular por Lotes , Células CACO-2 , Cromatografía Líquida de Alta Presión , Humanos , Lactobacillus/crecimiento & desarrollo , Lactobacillus/metabolismo , Malus/metabolismo , Estrés Oxidativo/efectos de los fármacos , Fenoles/análisis , Fenoles/farmacología , Especies Reactivas de Oxígeno/metabolismo , Saccharomyces cerevisiae/crecimiento & desarrollo , Saccharomyces cerevisiae/metabolismo , Espectrometría de Masas en Tándem
10.
Food Chem ; 336: 127551, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32795783

RESUMEN

The inhibitory effects of Portulaca oleracea L. (PO) and its flavonoid ingredients on the formation of heterocyclic amines (HAs) in roast beef patties were investigated. Ten HAs were found in control patties, and the total content was 212.73 ± 7.13 ng/g. With the addition of PO (1%, 5%, and 10%, w/w), HAs decreased by 62.39%, 68.03%, and 73.75%, respectively. The main flavonoid ingredients (rutin, hesperidin, and flavanone) also present a similar inhibitory effect. The Density Function Theory (DFT) methods were adopted to investigate the inhibitory mechanism. These ingredients bonded with the intermediate to block the formation of norharman. Both experimental and calculated data of the ingredients were analyzed on their HAs inhibitory capacity. Our results provide a novel and valuable strategy to reduce HAs via a low additive level of medicinal and edible plants. And the correlation between experimental and calculated data could be applied to predict the inhibitory ability of inhibitors.


Asunto(s)
Aminas/química , Flavonoides/química , Portulaca/química , Carne Roja/análisis , Aminas/análisis , Aminas/metabolismo , Animales , Antioxidantes/química , Bovinos , Cromatografía Líquida de Alta Presión , Teoría Funcional de la Densidad , Compuestos Heterocíclicos/química , Portulaca/metabolismo , Espectrometría de Masas en Tándem
11.
Food Chem ; 334: 127498, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32688179

RESUMEN

In this work, ultrasound-assisted enzymatic extraction (UAEE) conditions of umami compounds from Labeo rohita head (LRH) was optimised using the combination of fractional factorial design (FFD) and central composite design (CCD). Six factors that can potentially influence the degree of hydrolysis (DH), equivalent umami concentration (EUC), and extraction yield (EY) of umami extract were screened through FFD. The three most significant variables were further optimised using the CCD. The developed models were successfully fitted to the experimental data and used to determine optimal extraction conditions. The optimum DH (22.73%), EUC (6.61 g MSG/100 g protein), and EY (76.34%) of umami extract were achieved with a liquid-solid ratio of 3 (v/w), ultrasonication time of 19.20 min and hydrolysis time of 140.45 min. Besides, UAEE umami extract exhibited higher DH, EUC, EY, and umami taste compared to that of the conventional enzymatic extraction (CEE).


Asunto(s)
Productos Pesqueros/análisis , Sonicación , Aminoácidos/análisis , Animales , Cromatografía Líquida de Alta Presión , Cyprinidae , Proteínas de Peces/metabolismo , Hidrólisis , Proyectos de Investigación
12.
Food Chem ; 334: 127511, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32707367

RESUMEN

The sustainable exploitation of fruit and cereal processing is being conducted to produce novel food products with promising nutritional properties and high content in added value compounds. Herein, three bilberry fruit-based snacks supplemented with edible petals and fruits were characterized for their nutritional properties and chemical composition. The phenolic profile, antioxidant, antibacterial and hepatotoxic properties were analyzed. Protein (3-4 g/100 g dw) and carbohydrates (94.3-94.8 g/100 g dw) represented the major macronutrients. The combination of bilberry fruits with edible petals, calendula and rose, improved the nutritional and phytochemical input in organic acids and tocopherols content, respectively. Also, the supplementation with apple and goji fruits provided higher content in phenolic acids and anthocyanins (up to a 9-fold higher concentration, 199.7 µg/g of extract), also resulting in a higher antioxidant and antibacterial activities. The results obtained can contribute for the development of novel sustainable and healthier snacks for the food industry.


Asunto(s)
Valor Nutritivo , Vaccinium myrtillus/química , Antocianinas/química , Antioxidantes/química , Carbohidratos/análisis , Cromatografía Líquida de Alta Presión , Ácidos Grasos/análisis , Frutas/química , Frutas/metabolismo , Fenoles/análisis , Bocadillos , Espectrometría de Masa por Ionización de Electrospray , Tocoferoles/análisis , Vaccinium myrtillus/metabolismo
13.
Food Chem ; 334: 127484, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711263

RESUMEN

This study investigated the soymilk coagulation induced by fermented yellow whey (FYW), which is extensively used as a natural tofu coagulant in China. The aggregations involving proteins and isoflavone particles caused by FYW were analyzed using the proteomic technology and high-performance liquid chromatography, respectively. As indicated, the FYW-induced coagulation of soy proteins mainly occurred at pH 5.80-5.90. When the pH of soymilk decreased, the 7S ß, 11S A3 and some of 11S A1a subunits and SBP, Bd, lectin and TA aggregated the earliest, and later did the 11S A4, other 11S A1a, 11S A2 and 11S A1b subunits. The 7S α and α' subunits and TB showed an obvious delay in aggregation. Moreover, isoflavones in the form of aglycones were more likely to coprecipitate with proteins, compared with glycosides. These results could provide an important reference and assistance for future research on the development of traditional FYW-tofu.


Asunto(s)
Isoflavonas/análisis , Lactobacillales/crecimiento & desarrollo , Agregado de Proteínas/fisiología , Leche de Soja/química , Proteínas de Soja/análisis , Suero Lácteo/química , Reactores Biológicos , Cromatografía Líquida de Alta Presión , Electroforesis en Gel Bidimensional , Concentración de Iones de Hidrógeno , Proteómica , Leche de Soja/metabolismo , Suero Lácteo/metabolismo
14.
Food Chem ; 334: 127496, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711264

RESUMEN

With booming of computer technology, it is feasible to quantitatively extract valuable information from readily available image of objects, which dramatically reduces the cost and improves the efficiency. In this work, a structural classification method, based on data extracted from color formation process on thin layer chromatography (TLC) plates through computer processing, was established for natural products. Representative natural products exhibited good clustering and separation according to the extracted color feature, and structure of natural products can be classified by the distribution region in the color system. This method provides structural information for typical types of natural products directly from the formed color on TLC, which is very efficient and make portable device-based structure analysis of natural products possible. In addition, a general mechanism of color formation was proposed. This method is free from special instrument, high-throughput, and would facilitate large-scale screening of bioactives from natural sources.


Asunto(s)
Productos Biológicos/análisis , Cromatografía en Capa Delgada/métodos , Productos Biológicos/química , Cromatografía Líquida de Alta Presión , Análisis por Conglomerados , Color , Espectrometría de Masas
15.
Food Chem ; 334: 127517, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711266

RESUMEN

To facilitate selective breeding of polyphenol-rich peanuts, we looked for mass spectrometry-based proteomic evidence, investigating a subset of recombinant inbred lines (RILs) developed by the Australian peanut breeding program. To do this, we used label-free shotgun proteomics for protein and peptide quantitation, statistically analyzed normalized spectral abundance factors using R-package, as well as assayed important antioxidants. Results revealed statistically significant protein expression changes in 82 proteins classified between high or low polyphenols expressing RILs. Metabolic changes in polyphenol-rich RIL p27-362 point towards increased enzymatic breakdown of sugars and phenylalanine biosynthesis. The study revealed phenylpropanoid pathway overexpression resulting in increased polyphenols biosynthesis. Overexpression of antioxidant enzymes such as catalase, by 73.4 fold was also observed. A strong metabolic correlation exists with the observed phenotypic traits. Peanut RIL p27-362 presents a superior nutritional composition with antioxidant-rich peanut phenotype and could yield commercial profits. Data are available via ProteomeXchange with identifierPXD015493.


Asunto(s)
Arachis/metabolismo , Proteínas de Plantas/metabolismo , Polifenoles/química , Proteómica/métodos , Aminoácidos/metabolismo , Antioxidantes/química , Arachis/química , Biomarcadores/metabolismo , Cruzamiento , Catalasa/metabolismo , Cromatografía Líquida de Alta Presión , Fenotipo , Proteínas de Plantas/análisis , Polifenoles/aislamiento & purificación , Polifenoles/metabolismo , Extracción en Fase Sólida , Espectrometría de Masas en Tándem
16.
Food Chem ; 334: 127519, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32721832

RESUMEN

We aimed to characterize and quantify glucosinolate compounds and contents in broccoli, and a total of 80 genotypes and eight developmental organs were analyzed with UHPLC-Triple-TOF-MS. The method was validated in terms of performance, and the coefficients of determination (R2) were 0.97 and 0.99 for glucoraphanin and gluconapin, respectively. In 80 genotypes, twelve glucosinolates were found in broccoli florets ranging from 0.467 to 57.156 µmol/g DW, with the highest glucosinolate content being approximately 122-fold higher than the lowest value. The principal component of glucobrassicin, neoglucobrassicin and glucoraphanin explained 60.53% of the total variance. There were positive correlations among hydroxyglucobrassicin, methoxyglucobrassicin, glucobrassicin, glucoerucin, gluconasturtiin, glucoraphanin, and glucotropaeolin (P < 0.05). The root contained 43% of total glucosinolates in 80 genotypes, and glucoraphanin represented 29% of the total glucosinolate content in different organs. The mutant broccoli genotypes were found by analysis of gluconapin contents in different organs.


Asunto(s)
Brassica/metabolismo , Glucosinolatos/química , Espectrometría de Masa por Ionización de Electrospray/métodos , Brassica/genética , Cromatografía Líquida de Alta Presión , Genotipo , Glucosinolatos/análisis , Imidoésteres/análisis , Indoles/análisis
17.
Food Chem ; 336: 127713, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32768909

RESUMEN

The presence of multiple mycotoxins in the agricultural products poses a serious threat to the health of humans and animals. Citrinin (CIT) causes slow growth in animals and damages the kidney function. Zearalenone (ZEN) causes chronic poisoning, abnormal functioning and even death in animals. Herein, a dual fluorescent immunochromatographic assay (DF-ICA) based on europium nanoparticles (EuNPs) was developed for the simultaneous detection of CIT and ZEN in the corn samples. After optimization, the limits of detection (LODs), IC50 and average recoveries for the simultaneous determination of CIT and ZEN were 0.06 and 0.11 ng/mL, 0.35 and 0.76 ng/mL, from 86.3% to 111.6% and from 86.6% to 114.4%, respectively. Moreover, the DF-ICA was validated by high performance liquid chromatography (HPLC) analyses, and a satisfactory consistency was obtained. In brief, this work demonstrates the feasibility of DF-ICA for simultaneous monitoring of CIT and ZEN in the corn samples.


Asunto(s)
Citrinina/análisis , Contaminación de Alimentos/análisis , Inmunoensayo/métodos , Zea mays/química , Zearalenona/análisis , Animales , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/inmunología , Cromatografía Líquida de Alta Presión , Citrinina/inmunología , Europio/química , Fluorescencia , Análisis de los Alimentos/instrumentación , Análisis de los Alimentos/métodos , Humanos , Límite de Detección , Nanopartículas del Metal/química , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Zearalenona/inmunología
18.
Food Chem ; 336: 127660, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32771898

RESUMEN

Curcumin is a powerful coloring agent widely used in the food industry. Its extraction from the plant Curcuma longa is commonly done with aqueous solvent solutions. In contrast to the conventional extraction methods, the present study aimed to compare two different green and bio-based surfactant-free microemulsion (SFME) extraction systems, which are approved for food and yield a higher extracting power of curcuminoids. Two SFMEs, water/ethanol/triacetin and water/diacetin/triacetin, were investigated via dynamic light scattering. Curcumin solubility in binary mixtures consisting of ethanol/triacetin or diacetin/triacetin was studied both experimentally and theoretically using UV-Vis measurements and COSMO-RS. The SFMEs were further examined and compared to a common ethanol/water (80/20) extraction mixture with respect to their extracting ability using high performance liquid chromatography. The SFMEs containing ethanol were found to extract ~18% more curcuminoids than the SFMEs containing diacetin and ~53% more than the ordinary ethanol/water mixture.


Asunto(s)
Curcuma/química , Curcumina/química , Curcumina/aislamiento & purificación , Emulsiones/química , Cromatografía Líquida de Alta Presión/métodos , Curcumina/análisis , Diarilheptanoides/química , Dispersión Dinámica de Luz , Etanol/química , Tecnología Química Verde , Extractos Vegetales/química , Solubilidad , Solventes/química , Espectrofotometría Ultravioleta , Tensoactivos/química , Triacetina/química , Agua/química
19.
Food Chem ; 336: 127701, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32781354

RESUMEN

This study presents innovative research for comparison of the effect of the different dehydration techniques and methods of extraction on the antioxidant potential and bioactive compounds of Conilon and Arabica coffee flowers. The compounds were analyzed by high performance liquid chromatography and the antioxidant capacity evaluated by the 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide assays. Among the compounds evaluated, trigonelline, gallic acid, chlorogenic acid, and caffeine were identified, with trigonelline and caffeine being those with the highest concentration. The investigated factors significantly influenced the profile of the bioactive compounds identified, and the antioxidant capacity. The 92 °C infusion of freeze-dried Conilon coffee flowers, in general, showed greater antioxidant capacity by ABTS and DPPH assays, as well as total phenolic content. Lyophilization had a positive influence on maintaining the content of phenolic compounds and antioxidant capacity of the samples. Coffee flowers proved to be a potential raw material for making tea-like drinks.


Asunto(s)
Antioxidantes/farmacología , Coffea/química , Flores/química , Alcaloides/análisis , Antioxidantes/química , Cafeína/análisis , Ácido Clorogénico/análisis , Cromatografía Líquida de Alta Presión , Liofilización , Fenoles/análisis , Extractos Vegetales/química
20.
Food Chem ; 334: 127521, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32693333

RESUMEN

The objective of this study was to evaluate the optimal Subcritical Water Extraction (SWE) conditions of antioxidants and polyphenols from chestnut shells using Response Surface Methodology (RSM). A central composite design (CCD) was conducted to analyse the time (6-30 min) and temperature (51-249 °C) effects in antioxidant activity (ABTS, DPPH and FRAP) and Total Phenolic Compounds (TPC). TPC ranged from 315.21 to 496.80 mg gallic acid equivalents (GAE)/g DW; the DPPH from 549.23 to 1125.68 mg Trolox equivalents (TE)/g DW; ABTS varied between 631.16 and 965.45 mg ascorbic acid equivalents (AAE)/g DW and FRAP from 2793.95 to 11393.97 mg ferrous sulphate equivalents (FSE)/g DW. The optimal extraction conditions were 30 min/220 °C, revealing excelling scavenging efficiencies against HOCl (IC50 = 0.79 µg/mL) and O2- (IC50 = 12.92 µg/mL) without toxicity on intestinal cells (0.1 µg/mL). The phenolic composition revealed high amounts of pyrogallol and protocatechuic acid. SWE can be a useful extraction technique for the recovery of polyphenolics from chestnut shells.


Asunto(s)
Aesculus/química , Antioxidantes/química , Fenoles/química , Aesculus/metabolismo , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Humanos , Nueces/química , Nueces/metabolismo , Fenoles/análisis , Fenoles/farmacología , Extractos Vegetales/química , Polifenoles/análisis , Espectrometría de Masas en Tándem , Temperatura , Agua/química
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