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1.
Anim Biotechnol ; 35(1): 2371519, 2024 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-38990689

RESUMEN

The present study aimed to evaluate the effect of dry turmeric rhizomes on in vitro biogas production and diet fermentability. Turmeric rhizomes were included at gradually increased levels: 0, 0.5, 1, 1.5 and 2% of a diet containing per kg dr matter (DM): 500 g concentrate feed mixture, 400 g berseem hay and 100 g rice straw, and incubated for 48 h. Gas chromatography-mass spectrometry analysis showed that ar-turmerone, α-turmerone and ß-turmerone were the major bioactive compounds in the rhizomes. Turmeric rhizomes increased (p < 0.01) asymptotic gas production (GP) and rate and lag of CH4 production and decreased (p < 0.01) rate of GP, lag of GP, asymptotic CH4 production and proportion of CH4 production. Turmeric rhizome administration linearly increased (p < 0.01) DM and fiber degradability and concentrations of total short-chain fatty acids, acetic and propionic acids and ammonia-N and quadratically (p < 0.05) decreased fermentation pH. It is concluded that including up to 2% turmeric rhizomes improved in vitro ruminal fermentation and decreased CH4 production.


Asunto(s)
Curcuma , Fermentación , Metano , Rizoma , Curcuma/química , Rizoma/química , Animales , Metano/metabolismo , Rumen/metabolismo , Alimentación Animal/análisis , Dieta/veterinaria , Digestión/efectos de los fármacos
2.
Trop Anim Health Prod ; 56(6): 215, 2024 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-39004693

RESUMEN

This study evaluated the effect of cobiotic (CO) composed of organic fructans powder of Agave tequilana and turmeric powder of Curcuma longa L. as an alternative of antibiotic growth promoters (AGPs) on growth performance, blood parameters, intestinal pH, oxidative stress, and cytokines serum levels of broiler chickens. A total of 135 one-day-old Ross 308 broilers distributed to five experimental groups, which included starter or finisher standard diets without AGPs (CON), CON + 0.25 COLI-ZIN g/kg feed (AGP), CON + 0.1 g Agave fructans/kg feed (AF), CON + 0.5 g turmeric powder/kg feed (TP) and CON + 0.1 g AF + 0.5 g TP /kg feed (CO), for 49 days. AF followed by TP, decreased feed intake, obtaining the best FCR. AGP increased the heterophil-lymphocyte ratio compared to other groups. CO significantly decreased the pH of the cecal content. AF increased IL-10 levels, while TP decreased it. AF decreased the IL-1ß levels. The present study showed that including a cobiotic based on AF and TP or components separately in a broilers diet improved growth performance, modified intestinal and cecum pH, and stimulated the immune system, which suggests CO as a safe alternative to AGP.


Asunto(s)
Agave , Alimentación Animal , Pollos , Dieta , Suplementos Dietéticos , Fructanos , Estrés Oxidativo , Animales , Pollos/crecimiento & desarrollo , Pollos/inmunología , Pollos/sangre , Fructanos/administración & dosificación , Fructanos/farmacología , Alimentación Animal/análisis , Suplementos Dietéticos/análisis , Estrés Oxidativo/efectos de los fármacos , Agave/química , Dieta/veterinaria , Citocinas/sangre , Citocinas/metabolismo , Masculino , Curcuma/química , Fenómenos Fisiológicos Nutricionales de los Animales/efectos de los fármacos
3.
Molecules ; 29(12)2024 Jun 12.
Artículo en Inglés | MEDLINE | ID: mdl-38930859

RESUMEN

Turmeric (Curcuma longa) contains curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). Nevertheless, curcumin is the most researched active ingredient for its numerous pharmacological effects. We investigated the impact of these curcuminoids found in Ryudai gold, an approved cultivar of Curcuma longa, on wound healing, inflammation, and diabetes. Sub-planter injections of carrageenan induced acute paw inflammation in rats. The wound-healing ability of 1% curcuminoids was examined by making a 6 mm round wound on the shaved dorsum of the mice with a biopsy punch. A single intraperitoneal injection of streptozotocin (50 mg/kg) was used to induce diabetes in mice. Curcuminoids at a dose rate of 100 mg/kg body weight were used with feed and as a gastric gavage to treat diabetes and inflammation in experimental animals. Paw thickness was measured at 1, 3, and 6 h following carrageenan injection. After three hours, mean paw volume was 58% in carrageenan-injected mice, which was 35%, 37%, and 31% in the curcumin, DMC, and BDMC groups, respectively. Histopathology of the paw tissue demonstrated severe infiltration of inflammatory cells and thickening of the dermis, which were remarkably improved by the curcuminoids. The wound-healing abilities were significantly higher in the curcumin- (95.0%), DMC- (93.17%), and BDMC-treated (89.0%) groups, in comparison to that of the control (65.09%) group at day nine. There were no significant differences in wound-healing activity among the groups treated with 1% curcuminoids throughout the study. Streptozotocin-induced diabetes was characterized by an increased blood glucose (552.2 mg/dL) and decreased body weight (31.2 g), compared to that of the control rats (145.6 mg/dL and 46.8 g blood glucose and body weight, respectively). It also caused an increase in serum alanine aminotransferase (ALT; 44.2 U/L) and aspartate aminotransferase (AST; 55.8 U/L) compared to that of the control group (18.6 U/L and 20.1 U/L, respectively). Histopathological examination of the liver showed that diabetes caused hepatic cellular necrosis, congestion of the central vein, and parenchymatous degeneration. However, all three curcuminoids significantly decreased blood glucose levels, ALT, and AST and improved the histopathological score of the liver. These results evidenced that not only curcumin but also DMC and BDMC have potent anti-inflammatory, wound healing, and anti-diabetic efficacy, and the Ryudai gold variety of turmeric could be used as a functional food supplement.


Asunto(s)
Antiinflamatorios , Curcuma , Curcumina , Diabetes Mellitus Experimental , Hipoglucemiantes , Cicatrización de Heridas , Animales , Curcuma/química , Cicatrización de Heridas/efectos de los fármacos , Ratones , Ratas , Diabetes Mellitus Experimental/tratamiento farmacológico , Antiinflamatorios/farmacología , Antiinflamatorios/química , Hipoglucemiantes/farmacología , Hipoglucemiantes/química , Curcumina/farmacología , Curcumina/análogos & derivados , Masculino , Extractos Vegetales/farmacología , Extractos Vegetales/química , Carragenina , Inflamación/tratamiento farmacológico , Inflamación/patología , Diarilheptanoides/farmacología , Diarilheptanoides/química
4.
Sci Rep ; 14(1): 14709, 2024 06 26.
Artículo en Inglés | MEDLINE | ID: mdl-38926602

RESUMEN

Natural spices play an essential role in human nutrition and well-being. However, their processing on different scales can expose them to potential sources of contamination. This study aimed to describe the bacterial community genomic footprint in spices sold in Senegal. Spice samples were collected in August 2022 in Saint-Louis, Senegal. The genomic region coding bacterial 16S rRNA was then amplified and sequenced using Oxford Nanopore Technology (ONT). Sequencing was carried out on two batches of samples, one containing part of the "Local Spices or Herbs" (n = 10), and the other, a mixture of 7 spices, Curcuma, Thyme and the other part of the "Local Spices or Herbs" (n = 39). Results showed high bacterial diversity and the predominance of Escherichia coli and Salmonella enterica in samples, with total reads of 65,744 and 165,325 for the two batches, respectively. The sample category "Homemade mixture of food condiments ", which includes all "Local Spices or Herbs" samples, showed remarkable bacterial diversity. These were followed by Curcuma, a blend of 7 spices and thyme. Also, the different categories of spices studied show similarities in their bacterial composition. These results highlight the microbial community's highly diverse genomic profile, including pathogenic bacteria, in spice samples.


Asunto(s)
Metagenómica , ARN Ribosómico 16S , Especias , Especias/microbiología , Senegal , Metagenómica/métodos , ARN Ribosómico 16S/genética , Bacterias/genética , Bacterias/clasificación , Bacterias/aislamiento & purificación , Humanos , Metagenoma , Microbiota/genética , Curcuma/genética , Curcuma/microbiología
5.
Sci Rep ; 14(1): 13032, 2024 06 06.
Artículo en Inglés | MEDLINE | ID: mdl-38844676

RESUMEN

Green products such as plant tints are becoming more and more well-known worldwide due to their superior biological and ayurvedic properties. In this work, colorant from Amba Haldi (Curcuma aromatica) was isolated using microwave (MW), and bio-mordants were added to produce colorfast shades. Response surface methodology was used to develop a central composite design (CCD), which maximizes coloring variables statistically. The findings from 32 series of experiments show that excellent color depth (K/S = 12.595) was established onto MW-treated silk fabric (RS = 4 min) by employing 65 mL of radiated aqueous extract (RE = 4 min) of 5 pH cutting-edge the existence of 1.5 g/100 mL used sodium chloride at 75 °C for 45 min. It was discovered that acacia (keekar) extract (1%), pomegranate extract (2%), and pistachio extract (1.5%) were present before coloring by the use of bio-mordants. On the other hand, upon dyeing, acacia extract (1.5%), pomegranate extract (1.5%), and pistachio extract (2%) have all shown extremely strong colorfast colors. Comparatively, before dyeing, salts of Al3+ (1.5%), Fe2+ (2%), and TA (1.5%) gave good results; after dyeing, salts of Al3+ (1%) and Fe2+ (1.5%) and TA (2%) gave good results. When applied to silk fabric, MW radiation has increased the production of dyes recovered from rhizomes. Additionally, the right amount of chemical and biological mordants have been added, resulting in color fastness ratings ranging from outstanding to good. Therefore, the natural color extracted from Amba Haldi can be a sustainable option for the dyeing of silk fabric in the textile dyeing and finishing industries.


Asunto(s)
Colorantes , Curcuma , Extractos Vegetales , Rizoma , Seda , Curcuma/química , Rizoma/química , Colorantes/química , Extractos Vegetales/química , Seda/química , Microondas , Color , Tecnología Química Verde/métodos
6.
J Appl Oral Sci ; 32: e20230291, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38865512

RESUMEN

The prevalence of gingivitis is substantial within the general population, necessitating rigorous oral hygiene maintenance. OBJECTIVE: This study assessed a Garcinia indica (GI) fruit extract-based mouthrinse, comparing it to a 0.1% turmeric mouthrinse and a 0.2% Chlorhexidine (CHX) mouthrinse. The evaluation encompassed substantivity, staining potential, antimicrobial efficacy and cytocompatibility. METHODOLOGY: The study employed 182 tooth sections. For antimicrobial analysis, 64 extracted human teeth coated with a polymicrobial biofilm were divided into four groups, each receiving an experimental mouthrinse or serving as a control group with distilled water. Microbial reduction was assessed through colony forming units (CFU). Substantivity was evaluated on 54 human tooth sections using a UV spectrophotometer, while staining potential was examined on 64 tooth sections. Cytocompatibility was tested using colorimetric assay to determine non-toxic levels of 0.2% GI fruit extract, 0.1% Turmeric, and 0.2% CHX. RESULTS: Data were analysed with one-way ANOVA (α=0.05). Cell viability was highly significant (p<0.001) in the 0.2% GI group (64.1±0.29) compared to 0.1% Turmeric (40.2±0.34) and 0.2% CHX (10.95±1.40). For antimicrobial activity, both 0.2% GI (20.18±4.81) and 0.2% CHX (28.22±5.41) exhibited no significant difference (P>0.05) at end of 12 hours. However, 0.1% Turmeric showed minimal CFU reduction (P<0.001). Substantivity results at 360 minutes indicated statistically significant higher mean release rate in 0.1%Turmeric (12.47±5.84 ) when compared to 0.2% GI (5.02±3.04) and 0.2% CHX (4.13±2.25) (p<0.001). The overall discoloration changes (∆E) were more prominent in the 0.2% CHX group (18.65±8.3) compared to 0.2% GI (7.61±2.4) and 0.1% Turmeric (7.32±4.9) (P<0.001). CONCLUSION: This study supports 0.2% GI and 0.1% Turmeric mouth rinses as potential natural alternatives to chemical mouth rinses. These findings highlight viability of these natural supplements in oral healthcare.


Asunto(s)
Biopelículas , Clorhexidina , Curcuma , Frutas , Garcinia , Antisépticos Bucales , Higiene Bucal , Extractos Vegetales , Extractos Vegetales/farmacología , Humanos , Antisépticos Bucales/farmacología , Clorhexidina/farmacología , Garcinia/química , Curcuma/química , Biopelículas/efectos de los fármacos , Higiene Bucal/métodos , Frutas/química , Análisis de Varianza , Recuento de Colonia Microbiana , Reproducibilidad de los Resultados , Supervivencia Celular/efectos de los fármacos , Antiinfecciosos Locales/farmacología , Espectrofotometría Ultravioleta , Colorimetría , Ensayo de Materiales , Factores de Tiempo
7.
Food Funct ; 15(13): 6798-6824, 2024 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-38836693

RESUMEN

In recent decades, natural compounds derived from herbal medicine or dietary sources have played important roles in prevention and treatment of various diseases and have attracted more and more attention. Curcumin, extracted from the Curcumae Longae Rhizoma and widely used as food spice and coloring agent, has been proven to possess high pharmacological value. However, the pharmacological application of curcumin is limited due to its poor systemic bioavailability. As a major active metabolite of curcumin, tetrahydrocurcumin (THC) has higher bioavailability and stability than curcumin. Increasing evidence confirmed that THC had a wide range of biological activities and significant treatment effects on diseases. In this paper, we reviewed the research progress on the biological activities and therapeutic potential of THC on different diseases such as neurological disorders, metabolic syndromes, cancers, and inflammatory diseases. The extensive pharmacological effects of THC involve the modulation of various signaling transduction pathways including MAPK, JAK/STAT, NF-κB, Nrf2, PI3K/Akt/mTOR, AMPK, Wnt/ß-catenin. In addition, the pharmacokinetics, drug combination and toxicology of THC were discussed, thus providing scientific basis for the safe application of THC and the development of its dietary supplements and drugs.


Asunto(s)
Curcumina , Curcumina/farmacología , Curcumina/análogos & derivados , Curcumina/química , Humanos , Animales , Neoplasias/tratamiento farmacológico , Neoplasias/prevención & control , Neoplasias/metabolismo , Transducción de Señal/efectos de los fármacos , Enfermedades del Sistema Nervioso/tratamiento farmacológico , Enfermedades del Sistema Nervioso/prevención & control , Curcuma/química , Inflamación/tratamiento farmacológico , Inflamación/prevención & control , Enfermedades Metabólicas/prevención & control , Enfermedades Metabólicas/tratamiento farmacológico , Enfermedades Metabólicas/metabolismo
8.
Planta ; 260(1): 26, 2024 Jun 11.
Artículo en Inglés | MEDLINE | ID: mdl-38861179

RESUMEN

MAIN CONCLUSION: CaTPS2 and CaTPS3 were significantly expressed in flowers of Curcuma alismatifolia 'Shadow' and demonstrated bifunctional enzyme activity, CaTPS2 generated linalool and nerolidol as products, and CaTPS3 catalyzed ß-myrcene and ß-farnesene formation. This study presents the discovery and functional characterization of floral terpene synthase (TPS) genes in Curcuma alismatifolia 'Shadow', a cultivar renowned for its unique fragrance. Addressing the gap in understanding the genetic basis of floral scent in this species, we identified eight TPS genes through comprehensive transcriptome sequencing. Among these, CaTPS2 and CaTPS3 were significantly expressed in floral tissues and demonstrated bifunctional enzyme activity corresponding to the major volatile compounds detected in 'Shadow'. Functional analyses, including in vitro assays complemented with rigorous controls and alternative identification methods, elucidated the roles of these TPS genes in terpenoid biosynthesis. In vitro studies were conducted via heterologous expression in E. coli, followed by purification of the recombinant protein using affinity chromatography, enzyme assays were performed with GPP/FPP as the substrate, and volatile products were inserted into the GC-MS for analysis. Partially purified recombinant protein of CaTPS2 catalyzed GPP and FPP to produce linalool and nerolidol, respectively, while partially purified recombinant protein of CaTPS3 generated ß-myrcene and ß-farnesene with GPP and FPP as substrates, respectively. Real-time quantitative PCR further validated the expression patterns of these genes, correlating with terpenoid accumulation in different plant tissues. Our findings illuminate the molecular mechanisms underpinning floral fragrance in C. alismatifolia and provide a foundation for future genetic enhancements of floral scent in ornamental plants. This study, therefore, contributes to the broader understanding of terpenoid biosynthesis in plant fragrances, paving the way for biotechnological applications in horticulture plant breeding.


Asunto(s)
Monoterpenos Acíclicos , Transferasas Alquil y Aril , Curcuma , Flores , Sesquiterpenos , Transferasas Alquil y Aril/genética , Transferasas Alquil y Aril/metabolismo , Flores/genética , Flores/enzimología , Flores/metabolismo , Sesquiterpenos/metabolismo , Monoterpenos Acíclicos/metabolismo , Curcuma/genética , Curcuma/enzimología , Curcuma/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regulación de la Expresión Génica de las Plantas , Terpenos/metabolismo , Compuestos Orgánicos Volátiles/metabolismo , Filogenia , Odorantes
10.
Molecules ; 29(9)2024 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-38731583

RESUMEN

Xanthorrhizol, an important marker of Curcuma xanthorrhiza, has been recognized for its different pharmacological activities. A green strategy for selective xanthorrhizol extraction is required. Herein, natural deep eutectic solvents (NADESs) based on glucose and organic acids (lactic acid, malic acid, and citric acid) were screened for the extraction of xanthorrhizol from Curcuma xanthorrhiza. Ultrasound-assisted extraction using glucose/lactic acid (1:3) (GluLA) gave the best yield of xanthorrhizol. The response surface methodology with a Box-Behnken Design was used to optimize the interacting variables of water content, solid-to-liquid (S/L) ratio, and extraction to optimize the extraction. The optimum conditions of 30% water content in GluLA, 1/15 g/mL (S/L), and a 20 min extraction time yielded selective xanthorrhizol extraction (17.62 mg/g) over curcuminoids (6.64 mg/g). This study indicates the protective effect of GluLA and GluLA extracts against oxidation-induced DNA damage, which was comparable with those obtained for ethanol extract. In addition, the stability of the xanthorrhizol extract over 90 days was revealed when stored at -20 and 4 °C. The FTIR and NMR spectra confirmed the hydrogen bond formation in GluLA. Our study reported, for the first time, the feasibility of using glucose/lactic acid (1:3, 30% water v/v) for the sustainable extraction of xanthorrhizol.


Asunto(s)
Antioxidantes , Curcuma , Fenoles , Extractos Vegetales , Rizoma , Curcuma/química , Antioxidantes/química , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Rizoma/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Fenoles/química , Fenoles/aislamiento & purificación , Fenoles/farmacología , Disolventes Eutécticos Profundos/química , Ondas Ultrasónicas
11.
J Mass Spectrom ; 59(6): e5036, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38726689

RESUMEN

Turmeric and ginger are extensively employed as functional ingredients due to their high content of curcuminoids and gingerols, considered the key bioactive compounds found in these roots. In this study, we present an innovative and fast method for the assay of curcuminoids and gingerols in different foods containing the two spices, with the aim of monitoring the quality of products from a nutraceutical perspective. The proposed approach is based on paper spray tandem mass spectrometry coupled with the use of a labeled internal standard, which has permitted to achieve the best results in terms of specificity and accuracy. All the calculated analytical parameters were satisfactory; accuracy values are around 100% for all spiked samples and the precision data result lower than 15%. The protocol was applied to several real samples, and to demonstrate its robustness and reliability, the results were compared to those arising from the common liquid chromatographic method.


Asunto(s)
Curcuma , Alcoholes Grasos , Espectrometría de Masas en Tándem , Zingiber officinale , Zingiber officinale/química , Curcuma/química , Espectrometría de Masas en Tándem/métodos , Alcoholes Grasos/análisis , Reproducibilidad de los Resultados , Límite de Detección , Catecoles/análisis , Análisis de los Alimentos/métodos , Curcumina/análisis , Curcumina/análogos & derivados , Papel
13.
Plant Physiol Biochem ; 211: 108644, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38710114

RESUMEN

In this study, we have investigated the effect of carbon quantum dots (FM-CQDs) synthesized from marine fungal extract on Curcuma longa to improve the plant growth and curcumin production. The isolated fungus, Aspergillus flavus has produced a high amount of indole-3-acetic acid (IAA) (0.025 mg g-1), when treated with tryptophan. CQDs were synthesized from the A. flavus extract and it was characterized using ultraviolet visible spectrophotometer (UV-Vis) and high-resolution transmission electron microscopy (HR-TEM). The synthesized CQDs were excited at 365 nm in an UV-Vis and the HR-TEM analysis showed approximately 7.4 nm in size with a spherical shape. Both fungal crude extract (FCE) at 0-100 mg L-1 and FM-CQDs 0-5 mg L-1 concentrations were tested on C. longa. About 80 mg L-1 concentration FCE treated plants has shown a maximum height of 21 cm and FM-CQDs at 4 mg L-1 exhibited a maximum height of 25 cm compared to control. The FM-CQDs significantly increased the photosynthetic pigments such as total chlorophyll (1.08 mg g-1 FW) and carotenoids (17.32 mg g-1 FW) in C. longa. Further, antioxidant enzyme analysis confirmed that the optimum concentrations of both extracts did not have any toxic effects on the plants. FM-CQDs treated plants increased the curcumin content up to 0.060 mg g-1 by HPLC analysis. Semi quantitative analysis revealed that FCE and FM-CQDs significantly upregulated ClCURS1 gene expression in curcumin production.


Asunto(s)
Aspergillus flavus , Carbono , Curcuma , Curcumina , Puntos Cuánticos , Puntos Cuánticos/química , Curcuma/metabolismo , Curcuma/microbiología , Carbono/metabolismo , Carbono/farmacología , Curcumina/metabolismo , Curcumina/farmacología , Aspergillus flavus/metabolismo , Aspergillus flavus/crecimiento & desarrollo , Ácidos Indolacéticos/metabolismo , Endófitos/metabolismo
14.
Sci Rep ; 14(1): 10696, 2024 05 10.
Artículo en Inglés | MEDLINE | ID: mdl-38730068

RESUMEN

COVID-19, caused by SARS-CoV-2, affects neuronal cells, causing several symptoms such as memory loss, anosmia and brain inflammation. Curcuminoids (Me08 e Me23) and curcumin (CUR) are derived from Curcuma Longa extract (EXT). Many therapeutic actions have been linked to these compounds, including antiviral action. Given the severe implications of COVID-19, especially within the central nervous system, our study aims to shed light on the therapeutic potential of curcuminoids against SARS-CoV-2 infection, particularly in neuronal cells. Here, we investigated the effects of CUR, EXT, Me08 and Me23 in human neuroblastoma SH-SY5Y. We observed that Me23 significantly decreased the expression of plasma membrane-associated transmembrane protease serine 2 (TMPRSS2) and TMPRSS11D, consequently mitigating the elevated ROS levels induced by SARS-CoV-2. Furthermore, Me23 exhibited antioxidative properties by increasing NRF2 gene expression and restoring NQO1 activity following SARS-CoV-2 infection. Both Me08 and Me23 effectively reduced SARS-CoV-2 replication in SH-SY5Y cells overexpressing ACE2 (SH-ACE2). Additionally, all of these compounds demonstrated the ability to decrease proinflammatory cytokines such as IL-6, TNF-α, and IL-17, while Me08 specifically reduced INF-γ levels. Our findings suggest that curcuminoid Me23 could serve as a potential agent for mitigating the impact of COVID-19, particularly within the context of central nervous system involvement.


Asunto(s)
Antiinflamatorios , Antioxidantes , Antivirales , Tratamiento Farmacológico de COVID-19 , Curcumina , SARS-CoV-2 , Humanos , Curcumina/farmacología , Curcumina/análogos & derivados , Antioxidantes/farmacología , Antivirales/farmacología , SARS-CoV-2/efectos de los fármacos , SARS-CoV-2/fisiología , Antiinflamatorios/farmacología , Línea Celular Tumoral , Curcuma/química , Serina Endopeptidasas/metabolismo , COVID-19/virología , COVID-19/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , Extractos Vegetales/farmacología , Citocinas/metabolismo , NAD(P)H Deshidrogenasa (Quinona)/metabolismo , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Neuronas/virología
15.
Food Chem ; 452: 139561, 2024 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-38728897

RESUMEN

The utilization of essential oils as natural antioxidants and preservatives is limited by high volatility, poor water solubility, and long-term instability. To address this, a novel ultrasonic-assisted method was used to prepare and stabilize a nanoemulsion of turmeric essential oil-in-water, incorporating bioactive components extracted from Spirulina platensis. Ultrasonic treatment enhanced the extraction efficacy and nanoemulsion stability. Algal biomass subjected to ultrasonic treatment (30 min at 80% amplitude) yielded a dry extract of 73.66 ± 3.05%, with the highest protein, phenolic, phycocyanin, and allophycocyanin content, as well as maximum emulsifying activity. The resulting nanoemulsion (5% oil, 0.3% extract, 10 min ultrasonic treatment) showed reduced particle size (173.31 ± 2.24 nm), zeta potential (-36.33 ± 1.10 mV), low polydispersity index, and enhanced antioxidant and antibacterial properties. Rheology analysis indicated shear-thinning behavior, while microscopy and spectroscopy confirmed structural changes induced by ultrasonic treatment and extract concentration. This initiative developed a novel ultrasonic-assisted algal-based nanoemulsion with antioxidant and antibacterial properties.


Asunto(s)
Antibacterianos , Antioxidantes , Curcuma , Emulsiones , Aceites Volátiles , Spirulina , Spirulina/química , Antioxidantes/química , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Emulsiones/química , Aceites Volátiles/química , Aceites Volátiles/farmacología , Curcuma/química , Antibacterianos/farmacología , Antibacterianos/química , Tecnología Química Verde , Ultrasonido , Tamaño de la Partícula , Extractos Vegetales/química , Extractos Vegetales/farmacología , Extractos Vegetales/aislamiento & purificación , Agua/química
16.
Lett Appl Microbiol ; 77(6)2024 Jun 03.
Artículo en Inglés | MEDLINE | ID: mdl-38794899

RESUMEN

Non-antibiotic adjuncts may improve Helicobacter pylori infection control. Our aim was to emphasize curcumin benefits in controlling H. pylori infection. We discussed publications in English mostly published since 2020 using keyword search. Curcumin is the main bioactive substance in turmeric. Curcumin inhibited H. pylori growth, urease activity, three cag genes, and biofilms through dose- and strain-dependent activities. Curcumin also displayed numerous anticancer activities such as apoptosis induction, anti-inflammatory and anti-angiogenic effects, caspase-3 upregulation, Bax protein enhancement, p53 gene activation, and chemosensitization. Supplementing triple regimens, the agent increased H. pylori eradication success in three Iranian studies. Bioavailability was improved by liposomal preparations, lipid conjugates, electrospray-encapsulation, and nano-complexation with proteins. The agent was safe at doses of 0.5->4 g daily, the most common (in 16% of the users) adverse effect being gastrointestinal upset. Notably, curcumin favorably influences the intestinal microbiota and inhibits Clostridioides difficile. Previous reports showed the inhibitory effect of curcumin on H pylori growth. Curcumin may become an additive in the therapy of H. pylori infection, an adjunct for gastric cancer control, and an agent beneficial to the intestinal microbiota. Further examination is necessary to determine its optimal dosage, synergy with antibiotics, supplementation to various eradication regimens, and prophylactic potential.


Asunto(s)
Antibacterianos , Curcuma , Curcumina , Infecciones por Helicobacter , Helicobacter pylori , Curcumina/farmacología , Infecciones por Helicobacter/tratamiento farmacológico , Infecciones por Helicobacter/microbiología , Helicobacter pylori/efectos de los fármacos , Helicobacter pylori/genética , Humanos , Antibacterianos/farmacología , Microbioma Gastrointestinal/efectos de los fármacos
17.
Altern Ther Health Med ; 30(4): 18-23, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38702159

RESUMEN

Objective: Curcuminoids, the major component of which is curcumin, are natural polyphenolic compounds from the rhizome of Curcuma longa Linn. and possess extensive biopharmacological properties that are limited in humans due to poor bioavailability. Currently, most commercial bioavailable turmeric extracts use synthetic excipients or the addition of piperine to enhance bioavailability, and are needed in multiple daily doses to achieve clinical efficacy. This study was conducted to compare the bioavailability of a natural, water-dispersible turmeric extract containing 60% natural curcuminoids, the test product, WDTE60N (1 × 250 mg per day), with the reference product, turmeric extract capsules (500 mg curcuminoids and 5 mg piperine, CPC; 3 × 500 mg per day). Methods: Sixteen healthy adult male subjects fasted overnight for 10 hours and then were dosed with either one capsule of the test product WDTE60N or three capsules of reference product CPC orally (One capsule administered at every 6 hours interval i.e. at 0.00 hrs, 6.00 hrs and at 12.00 hrs) in each study period. Blood sampling before and after dosing was carried out at defined time points at -12.00, -02.00, 00.00 (within 10 minutes prior to dosing) hours in morning before dosing and post-dose (First dose) at 00.50, 01.00, 02.00, 03.00, 04.00, 05.00, 06.50, 07.00, 08.00, 09.00, 10.00, 11.00, 12.50, 13.00, 14.00, 16.00, 18.00, 20.00 and 24.00 hours in each period. Plasma concentration of curcuminoids was determined using a validated liquid chromatography with tandem mass spectrometry bioanalytical method. Results: The Cmax (GLSM) for the test product WDTE60N was observed to be 74.56 ng/mL; and same for the reference CPC was 22.75 ng/mL. AUC0-t (GLSM) for test WDTE60N was 419.00 h∙ng/mL; and for reference CPC it was 359.86 h∙ng/mL for total curcuminoids. Conclusion: The test formulation WDTE60N showed improved relative absorption and equivalent exposure at a 10-fold-lower dose of actives than the reference formulation CPC.


Asunto(s)
Alcaloides , Benzodioxoles , Estudios Cruzados , Curcuma , Curcumina , Piperidinas , Extractos Vegetales , Humanos , Masculino , Extractos Vegetales/farmacología , Extractos Vegetales/farmacocinética , Curcuma/química , Adulto , Alcaloides/farmacocinética , Alcaloides/farmacología , Benzodioxoles/farmacocinética , Benzodioxoles/farmacología , Curcumina/farmacocinética , Curcumina/farmacología , Piperidinas/farmacocinética , Piperidinas/farmacología , Disponibilidad Biológica , Adulto Joven , Alcamidas Poliinsaturadas/farmacología , Alcamidas Poliinsaturadas/farmacocinética
18.
Photochem Photobiol Sci ; 23(6): 1179-1194, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38771468

RESUMEN

In this study, photostability and photodynamic antimicrobial performance of dye extracts from Hibiscus sabdariffa (HS) calyces, Sorghum bicolor (SB) leaf sheaths, Lawsonia inermis (LI) leaves and Curcuma longa (CL) roots were investigated in Acetate-HCl (AH) Buffer (pH 4.6), Tris Base-HCl (TBH) Buffer (pH 8.6), distilled water (dH2O), and Phosphate Buffer Saline (PBS, pH 7.2) using Bacillus subtilis as model for gram positive bacteria, Escherichia coli as model for gram negative bacteria, phage MS2 as model for non-envelope viruses and phage phi6 as model for envelope viruses including SARS CoV-2 which is the causative agent of COVID-19. Our results showed that the photostability of the dye extracts is in the decreasing order of LI > CL > SB > HS. The dye extract-HS is photostable in dH2O but bleaches in buffers-AH, TBH and PBS. The rate of bleaching is higher in AH compared to in TBH and PBS. The bleaching and buffers affected the photodynamic and non-photodynamic antimicrobial activity of the dye extracts. The photodynamic antibacterial activity of the dye extracts is in the decreasing order of CL > HS > LI > SB while the non-photodynamic antibacterial activity is in the decreasing order of LI > CL > HS > SB. The non-photodynamic antiviral activity pattern observed is the same as that of non-photodynamic antibacterial activity observed. However, the photodynamic antiviral activity of the dye extracts is in the decreasing order of CL > LI > HS > SB. Given their performance, the dye extracts maybe mostly suitable for environmental applications including fresh produce and food disinfection, sanitation of hands and contact surfaces where water can serve as diluent for the extracts and the microenvironment is free of salts.


Asunto(s)
Extractos Vegetales , Extractos Vegetales/química , Extractos Vegetales/farmacología , Sorghum/química , Hibiscus/química , Curcuma/química , Escherichia coli/efectos de los fármacos , Levivirus/efectos de los fármacos , Fármacos Fotosensibilizantes/farmacología , Fármacos Fotosensibilizantes/química , Bacillus subtilis/efectos de los fármacos , Desinfección , Antibacterianos/farmacología , Antibacterianos/química , SARS-CoV-2/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Colorantes/química , Colorantes/farmacología , COVID-19 , Hojas de la Planta/química , Antiinfecciosos/farmacología , Antiinfecciosos/química , Luz
19.
ACS Appl Bio Mater ; 7(6): 3854-3864, 2024 Jun 17.
Artículo en Inglés | MEDLINE | ID: mdl-38820558

RESUMEN

Nanotechnology offers an innovative application as an eco-friendly food packaging film fabricated along with a degradable active mixture (AM). The AM is an assortment of alloyed metal oxide nanoparticles (Ag-ZnO), citron powder (AA), and Curcuma peel powder (CPP). Alloyed nanoparticles (NPs) were observed to exhibit a hexagonal structure from the experimental X-ray diffraction. Compositional and morphological study of the NPs (22.69 nm) and AM (32 nm) was done using energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and ζ- potential was observed to be -14.7 mV, indicating the stability of NPs. The prepared film was observed to be more effective with antibacterial analysis against Escherichia coli, exhibiting 72% of inhibition and antioxidant activity with IC50: 51.56% using the 2,2 diphenyl-1-picrylhydrazyl (DPPH) assay. Film 1, Film 2, Film 3, and Film 4 were fabricated with the AM and observed to be perfectly encapsulated by PVA using XRD. FESEM images of the film exhibit the aggregation of NPs with biocomposites in perfect distribution. The mechanical properties such as Young's modulus, elongation at break, tensile strength, and ultimate tensile strength (UTS- 5.37 MPa) were experimented for the films. The degradation rate was observed to be 6.12% for film 1 using the soil burial method. The study emphasizes that NPs along with biocomposite upgrade the sustainability of the packaging film with improved mechanical and physicochemical properties. The synthesized film with biomaterials could be used as a "green" food package to store fruits, vegetables, and sweets in the food industry.


Asunto(s)
Antibacterianos , Materiales Biocompatibles , Escherichia coli , Ensayo de Materiales , Tamaño de la Partícula , Plata , Óxido de Zinc , Óxido de Zinc/química , Óxido de Zinc/farmacología , Escherichia coli/efectos de los fármacos , Antibacterianos/química , Antibacterianos/farmacología , Antibacterianos/síntesis química , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Materiales Biocompatibles/síntesis química , Plata/química , Plata/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Antioxidantes/síntesis química , Pruebas de Sensibilidad Microbiana , Curcuma/química , Embalaje de Alimentos , Nanopartículas del Metal/química , Tecnología Química Verde , Citrus/química
20.
Zhongguo Zhong Yao Za Zhi ; 49(7): 1872-1881, 2024 Apr.
Artículo en Chino | MEDLINE | ID: mdl-38812199

RESUMEN

This study aims to identify the active constituents of essential oil from the rhizomes of Curcuma phaeocaulis for the treatment of dysmenorrhea. The compounds were separated and purified by molecular distillation, silica gel and Sephadex LH-20 column chromatography, preparative thin layer chromatography, and semi-preparative high performance liquid chromatography. Their structures were identified by mass spectrometry and nuclear magnetic resonance spectroscopy. The animal model of primary dysmenorrhea and the contraction model of isolated uterine smooth muscle of rats were established to examine the active constituents in the essential oil for treating dysmenorrhea. Six sesquiterpenes were isolated and identified as dehydrocommiterpene A(1), comosone Ⅱ(2), 5α(H)-eudesma-3(4),7(11)-dien-9ß-ol-6-one(3), guaia-6(7)-en-11-ol(4), curcumenol(5), and isocurcumenol(6), among which compound 1 was a novel compound. The animal experiments showed that the essential oil from C. phaeocaulis significantly lowered the level of PGF_(2α) in uterine tissue compared with the model group. The experiment with the contraction model of isolated uterine smooth muscle demonstrated that the components with high boiling points outperformed those with low boiling points in relaxing the uterine smooth muscle, and compounds 1, 2, 5, and 6 isolated from the fraction with a high boiling point had the effect of relaxing the uterine smooth muscle. Among them, compounds 5 and 6 inhibited the extracellular Ca~(2+) influx and intracellular Ca~(2+) release to relax the uterine smooth muscle. In conclusion, the components with high boiling points and sesquiterpenes are the active components in the essential oil of C. phaeocaulis for treating dysmenorrhea.


Asunto(s)
Curcuma , Dismenorrea , Aceites Volátiles , Dismenorrea/tratamiento farmacológico , Femenino , Aceites Volátiles/química , Aceites Volátiles/farmacología , Animales , Curcuma/química , Ratas , Ratas Sprague-Dawley , Humanos , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Útero/efectos de los fármacos , Rizoma/química
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