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1.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4677-4684, 2019 Nov.
Artículo en Chino | MEDLINE | ID: mdl-31872664

RESUMEN

Chinese herbal medicine ultrafine powder has become a research hotspot for the addition of cosmetic raw materials. Dendrobium candidum is a traditional Chinese herbal medicine. Its extract and stem extract are already cosmetic raw materials and its water extract has the effect of preventing photoaging,but D. candidum ultrafine powder has not been accepted as a raw material for cosmetics,and no relevant research on photoaging prevention has been reported. In this experiment,the ultra-fine powder and fine powder of D. candidum to prevent photoaging were observed and compared,and its mechanism of action was discussed to provide a basis for the prevention of skin photoaging products. Seventy-two female ICR mice were randomly divided into normal group,model group,solvent group,titanium dioxide(Ti O2) group,isooctyl salicylate(2-ES) group,D. candidum ultrafine powder 1(DP1),ultrafine powder 2(DP2) and fine powder(DP3) groups. The photoaging model was established by ultraviolet irradiation for 8 weeks,and the model was intervened while modeling. The skin wrinkle grade,elastic parameters,skin microcirculation blood flow,skin structure and pathological changes(skin thickness,skin collagen fiber,elastic fiber) were observed,the skin transforming growth factor-ß1(TGF-ß1),Smad3 levels were determined,and the type Ⅰ and type Ⅲ collagen,matrix metalloproteinase-1(MMP-1),activated protein-1(AP-1),VEGF expression were detected. The results showed that ultrafine powder(DP1,DP2) significantly reduced the wrinkle level and skin blood flow of the model mice(P<0. 05,P<0. 01); DP1,DP2 and DP3 could significantly reduce the thickness of the epidermis(P<0. 001),improve collagen fiber,elastic fiber hyperplasia,and distortion and decrease VEGF expression,and DP1 is better than DP2 and DP3; each group could up-regulate type Ⅰ collagen,down-regulate type Ⅲ collagen,AP-1,MMP-1 protein expression,and DP1 improvement optimal. However,it has no obvious effect on TGF-ß1 and Smad3. The ultrafine powder and fine powder of D. candidum have certain preventive effect on photoaging,and the effect of ultrafine powder is better than that of fine powder. Ultrafine powder may down-regulate the expression of type Ⅲ collagen,AP-1 and MMP-1 by up-regulating type Ⅰ collagen. Inhibition of collagen degradation plays a role in preventing photoaging.


Asunto(s)
Dendrobium , Envejecimiento de la Piel , Animales , Femenino , Ratones , Ratones Pelados , Ratones Endogámicos ICR , Piel , Rayos Ultravioleta
2.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4896-4904, 2019 Nov.
Artículo en Chino | MEDLINE | ID: mdl-31872598

RESUMEN

Previous studies of Dendrobium officinale on anti-hypertension effect always focused only on the blood pressure,while polysaccharides of D. officinale( DOP) have been traditionally considered as one of the main effective substances. This study aimed to evaluate the effect of ethanol extract from D. officinale( DOE) on blood pressure,Glu and lipid profile in metabolic hypertensive rats induced by comprehensive dietary factors,and elucidate the composition of effective fractions from DOE. A metabolic hypertension model of rat induced by high-sugar,high-fat diet and alcohol drinking was adopted to evaluate the effect of DOE on hypertension and other metabolic disorders. Blood pressure,Glu and lipid profile were detected to find the features and differences of DOE and DOP on metabolic hypertension. Furthermore,DOE was separated with three different common solvents according to the polarity. Along with blood pressure,Glu,UA and lipid profile,hemorheology,oxidative index and aortas structure changes were adopted to evaluate the comprehensive effects of the most effective fractions on metabolic hypertension. Finally,HPLC-DAD-MS was adopted to identify the components of the most effective fraction. The SBP and Glu of models were decreased significantly after administration of DOE and DOP for 6 weeks,while TG in DOE groups also reduced dramatically. The DOE was separated with ether,n-butanol respectively and named NAF,NBF and NCF. SBP,TG,Glu,UA of model rats were decreased significantly after 4 weeks administration with NBF. The level of MDA in serum was down-regulated,while GSH-Px and T-AOC were up-regulated obviously after 12 weeks.And the blood viscosity also obviously decreased,with less collagen deposition of aortas by Masson's trichrome staining. NBF was mainly composed of phenols and flavone C-glycosides,whose aglycone was apigenin,and monosaccharide was connected to C-6 and C-8. Ethanol extract from D.officinale has an positive effect in alleviating hypertension and metabolic disorders in metabolic hypertension. Medium polarity fraction was the effective fraction of alcohol extraction from D. officinale,and mainly composed of phenols and flavone C-glycosides.


Asunto(s)
Dendrobium , Hipertensión/tratamiento farmacológico , Extractos Vegetales/uso terapéutico , Animales , Presión Sanguínea , Etanol , Extractos Vegetales/farmacología , Ratas
3.
Zhongguo Zhong Yao Za Zhi ; 44(18): 3954-3959, 2019 Sep.
Artículo en Chino | MEDLINE | ID: mdl-31872730

RESUMEN

Through investigation,it was found that the main disease of leaves was grey mold on Dendrobium officinale in Hubei province,which has a great impact on the yield and quality of D. officinale. The identification of morphological and molecular biological was used to prove that the pathogen was Botrytis cinerea. Through test the effect of 5 plant source fungicides and 4 antibiotic fungicides on mycelial growth of strain HS1,which proved 0. 3% eugenol had the best inhibitory effect,EC50 was 0. 29 mg·L-1,the second was1% osthol and EC50 was 1. 12 mg·L-1,the EC50 of 0. 5% matrine was 9. 16 mg·L-1,the EC50 of the other six fungicides was higher than 10 mg·L-1. The field control effect test proved that 0. 3% eugenol had the best control effect,reaching 89. 44%,secondly for 1%osthole,which was 77. 17%,0. 5% matrine was in the third place with 62. 37% of effective rate. However,the control effect of the other fungicides was less than 60%. The three plant-derived fungicides were safe for the produce of D. officinale and showed no phytotoxicity. The effect of these fungicides on the growth of D. candidum was tested,and proved that all the fungicides were safe and harmless to D. candidum. This study provides a research basis for the safe and effective prevention and control gray mold of D. officinale.


Asunto(s)
Botrytis/patogenicidad , Dendrobium/microbiología , Fungicidas Industriales , Enfermedades de las Plantas/microbiología , Alcaloides , Botrytis/efectos de los fármacos , Cumarinas , Eugenol , Enfermedades de las Plantas/prevención & control , Hojas de la Planta/microbiología , Quinolizinas
4.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3221-3225, 2019 Aug.
Artículo en Chino | MEDLINE | ID: mdl-31602875

RESUMEN

The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum. In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation. The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method. The contents of starches,non-starch polysaccharides and monosaccharides were analyzed. In addition,the system suitability was tested. Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20. 9%,58. 8% and 1. 6% respectively. The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable. Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum. Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.


Asunto(s)
Dendrobium/química , Polisacáridos/análisis , Monosacáridos/análisis , Fitoquímicos/análisis , Almidón/análisis
5.
Plant Sci ; 287: 110179, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31481215

RESUMEN

Stigmatic mucilage plays a crucial role in pollen-grain adhesion on the stigma in flowering plants. Little information is available regarding mucilage biosynthesis in orchid plants. In the present study, stigmatic mucilage is rich in galactose-containing polysaccharides, mainly consisted of galactose and arabinose in Dendrobium officinale. Thirteen galactosyltransferases involved in biosynthesis of the ß-1,3-galactose linkage polysaccharides, belonging to the CAZY GT31 family, were identified from D. officinale genome. A positive correlation between the mucilage content and the DoGALT2 expression at different stages was observed. DoGALT2 expressed overall sampled tissues with the highest in D. officinale stigmatic mucilage that contributes to pollen adhesion and elongation. DoGALT2 was targeted to Golgi, and had a GALT domain (PF01762) that was homologous to the characterized GALT2 in Arabidopsis. Compared to wild-type Arabidopsis, DoGALT2 overexpressing plants showed a higher content of galactose and galactose-containing alcohol-insoluble residues, and enhanced tolerance to abiotic stress. DoGALT2 complemented Arabidopsis GALT2 mutant (galt2-1), with an equivalent galactose with wild-type Arabidopsis but significantly higher than galt2-1. These findings provide evidence that DoGALT2 might be involved in regulating the biosynthesis of galactose-containing polysaccharides during D. officinale pollen development.


Asunto(s)
Dendrobium/enzimología , Flores/enzimología , Galactosiltransferasas/metabolismo , Genes de Plantas/fisiología , Mucílago de Planta/biosíntesis , Proteínas de Plantas/metabolismo , Arabidopsis , Clonación Molecular , Dendrobium/genética , Dendrobium/metabolismo , Flores/química , Flores/metabolismo , Galactosa/metabolismo , Galactosiltransferasas/genética , Mucílago de Planta/química , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Polisacáridos/análisis , Transcriptoma
6.
Fitoterapia ; 138: 104351, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31476401

RESUMEN

Three new sesquiterpenoids, (+)-(1R,2S,3R,4S,5R,6S,9R)-3,11,12-trihydroxypicrotoxane-2(15)-lactone (1), (-)-(1S,2R,3S,4R,5S,6R,9S,12R)-3,11,13-trihydroxypicrotoxane-2(15)-lactone (2), and (+)-(1R,5R,6S,8R,9R)-8,12-dihydroxy-copacamphan-3-en-2-one (3), together with five known compounds, were isolated from the n-butanol soluble fraction of a 95% EtOH extract of the stems of Dendrobium nobile. Their structures were determined by extensive spectroscopic analysis. Particularly, to solve difficult stereochemical problems, electronic circular dichroism calculations, NMR data calculations, and a single-crystal X-ray diffraction were performed. Interestingly, compounds 1 and 2 were picrotoxinin-type sesquiterpenoids with an unusual C15,2-lactone ring. All new sesquiterpenoids (1-3) showed a significant neuroprotective activity against H2O2-induced oxidative damage in PC12 cells. Notably, at 25 and 50 µM, compounds 1 and 2 showed the best protective effects, even better than the positive control (vitamin E).


Asunto(s)
Dendrobium/química , Fármacos Neuroprotectores/farmacología , Tallos de la Planta/química , Sesquiterpenos/farmacología , Animales , China , Estructura Molecular , Fármacos Neuroprotectores/aislamiento & purificación , Células PC12 , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología , Ratas , Sesquiterpenos/aislamiento & purificación
7.
Molecules ; 24(17)2019 Aug 23.
Artículo en Inglés | MEDLINE | ID: mdl-31450800

RESUMEN

Two new indolizidine alkaloids, crepidatumines C (1) and D (2), together with crepidine (3), isocrepidamine (4), and crepidamine (5) were isolated from the Dendrobium crepidatum Lindl. ex Paxt. X-ray diffraction experiments established the absolute configurations of known compounds 3 and 4. The planar structures and relative configurations of new compounds 1 and 2 were elucidated by extensive spectra analysis including HR-ESI-MS, NMR (1H, 13C, 1H-1H COSY, HSQC, HMBC, and NOESY spectra), and the absolute configurations of 1 and 2 were suggested on the basis of possible biosynthetic pathways. The biological results confirmed that isocrepidamine (4) displayed a potent hypoglycemic effect in vitro without cytotoxicity.


Asunto(s)
Alcaloides/química , Alcaloides/farmacología , Dendrobium/química , Indolicidinas/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Humanos , Hipoglucemiantes/química , Hipoglucemiantes/farmacología , Espectroscopía de Resonancia Magnética , Estructura Molecular , Difracción de Rayos X
8.
J Chromatogr A ; 1607: 460388, 2019 Dec 06.
Artículo en Inglés | MEDLINE | ID: mdl-31351593

RESUMEN

Qualitative and quantitative analysis of polysaccharides in herb formula remain challenge due to the limited choices of analytical methods concerning the intrinsic characteristics of large molecular mass. Herein, an oligosaccharide-marker approach was newly developed for quality assessment of polysaccharides in herbal materials, using Dendrobium officinale as a case study. This method involved partial acid hydrolysis of D. officinale polysaccharide (DOP) followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Two ABEE-labeled oligosaccharides namely, Te-Man-ABEE and Pen-Man-ABEE, were selected as chemical markers due to their high specificity in herb formula. The linear relationship between the content of these two markers and the content of DOP was then successfully established respectively. The linear relationship was further transformed to that between peak area of chemical markers and DOP content so that chemical markers were not necessary to be isolated for analysis. This linear relationship was systemically validated in terms of precision and accuracy. The results showed that these two oligosaccharide-markers presented a good linear relationship with DOP (R2 ≥ 0.997) in the range of 0.68-16.02 µg. These markers also demonstrated satisfactory precision (RSD < 7.0%), and recovery (91.41%-118.30%) in real sample determination. Additionally, there was no significant difference between the results given by the two chemical markers as the RSD values were not more than 7.0%. While concerning the results given by the oligosaccharide-markers and the previously-published polysaccharide marker, the RSD value was not more than 6.4%. These suggest that the oligosaccharide-marker approach is a simple, quick, and reliable method to qualitatively and quantitatively determine of specific polysaccharide in herb formula.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Dendrobium/química , Espectrometría de Masas/métodos , Oligosacáridos/química , Extractos Vegetales/química , Fluorescencia , Hidrólisis , Peso Molecular
9.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1789-1792, 2019 May.
Artículo en Chino | MEDLINE | ID: mdl-31342703

RESUMEN

In order to scientifically prevent and control Dendrobium catenatum southern blight disease,the main factors related to this disease occurrence,the pathogen( Sclerotium delphinii),environmental factors( temperature and humidity) and D. catenatum germplasms,were investigated. The results showed that reaching 25-30 ℃ temperature and over 95% humidity simultaneously should be the main conditions for the occurrence and prevalence of D. catenatum southern blight disease. Moreover,the S. delphinii-infected plants and their contaminated substrates were the disease spreading sources. Therefore,removing the infected plants,dealing with the contaminated substrates,keeping air ventilation,and reducing air humidity are the effective ways to prevent and control the occurrence and prevalence of D. catenatum southern blight disease. The research also indicated that D. catenatum has different resistances to the southern blight disease depending on germplasm. The present study lays important foundations for the breeding of D. catenatum diseaseresistant varieties and the further analysis of the infection and resistance mechanisms underlying southern blight disease.


Asunto(s)
Basidiomycota/patogenicidad , Dendrobium/microbiología , Enfermedades de las Plantas/microbiología , Humedad , Temperatura Ambiental
10.
Carbohydr Polym ; 222: 115028, 2019 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-31320099

RESUMEN

A homogenous polysaccharide (GXG) from Dendrobium huoshanense with stable digestive behavior and effective immunoregulatory function was employed to explore its underlying molecular basis regulating intestinal mucosal immune response from the view of interaction between GXG and intestinal epithelial cells (IECs). Using in vitro established co-culture system consisting of IECs and lamina propria cells (LPCs), we found the immune response of LPCs could be effectively regulated by GXG-stimulated IECs, and three cytokines including IL-6, MCP-1 and CINC-1 produced from GXG-stimulated IECs were the main factors involved in modulating immune response of LPCs. Toll-like receptor 4 (TLR4) was identified as an essential receptor for IECs to directly bind GXG. Receptor intervention experiments demonstrated that TLR4 mediated GXG-induced activation of IECs, which further induces immunomodulating effects on LPCs. These results suggest that GXG could modulate the immune response in LPCs by the direct interaction with IECs via TLR4.


Asunto(s)
Células Epiteliales/efectos de los fármacos , Inmunidad Celular/efectos de los fármacos , Factores Inmunológicos/farmacología , Mucosa Intestinal/efectos de los fármacos , Polisacáridos/farmacología , Receptor Toll-Like 4/metabolismo , Animales , Línea Celular , Citocinas/metabolismo , Células Dendríticas/efectos de los fármacos , Dendrobium/química , Factores Inmunológicos/metabolismo , Linfocitos/efectos de los fármacos , Masculino , Polisacáridos/metabolismo , Ratas Sprague-Dawley
11.
Genetica ; 147(3-4): 303-313, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31292836

RESUMEN

Dendrobium officinale, a herb with highly medicinal and ornamental value, is widely distributed in China. MADS-box genes encode transcription factors that regulate various growth and developmental processes in plants, particular in flowering. However, the MADS-box genes in D. officinale are largely unknown. In our study, expression profiling analyses of selected MADS-box genes in D. officinale were performed. In total, 16 DnMADS-box genes with full-length ORF were identified and named according to their phylogenetic relationships with model plants. The transient expression of eight selected MADS-box genes in the epidermal cells of tobacco leaves showed that these DnMADS-box proteins localized to the nucleus. Tissue-specific expression analysis pointed out eight flower-specific expressed MADS-box genes in D. officinale. Furthermore, expression patterns of DnMADS-box genes were investigated during the floral transition process. DnMADS3, DnMADS8 and DnMADS22 were significantly up-regulated in the reproductive phase compared with the vegetative phase, suggesting putative roles of these DnMADS-box genes in flowering. Our data showed that the expressions of MADS-box genes in D. officinale were controlled by diverse exogenous phytohormones. Together, these findings will facilitate further studies of MADS-box genes in Orchids and broaden our understanding of the genetics of flowering.


Asunto(s)
Dendrobium/genética , Proteínas de Dominio MADS/genética , Proteínas de Plantas/genética , China , Dendrobium/metabolismo , Flores/metabolismo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Proteínas de Dominio MADS/metabolismo , Especificidad de Órganos , Filogenia , Proteínas de Plantas/metabolismo
13.
Molecules ; 24(14)2019 Jul 23.
Artículo en Inglés | MEDLINE | ID: mdl-31340453

RESUMEN

Dendrobium officinale is a herb in traditional Chinese medicine where D. officinale polysaccharides (DOP) are the main active ingredient. This study aimed at evaluating DOP efficiency at inhibiting 1-Methyl-2-nitro-1-nitrosoguanidine (MNNG) induced precancerous lesions of gastric cancer (PLGC) in rats through the Wnt/b-catenin pathway and analyzing the variations of serum endogenous metabolites. PLGC was established in male Sprague-Dawley (SD) rats by administering 150 µg/mL MNNG in drinking water for 7 months and giving 0.1 mL of 10% NaCl once weekly during the initial 20 weeks. Treatment with DOP inhibited the progress of PLGC through decreasing the expression of ß-catenin by immunohistochemical analysis. The futher study indicated DOP downregulated gene expression of Wnt2ß, Gsk3ß, PCNA, CyclinD1, and ß-catenin, as well as protein expression of Wnt2ß, PCNA, and ß-catenin. On the other hand, there were nine endogenous metabolites identified after the DOP treatment. Among these, the most significant one is betaine because of its strong antioxidant activity, leading to an anti-tumor effect. DOP can inhibit MNNG-induced PLGC models via regulating Wnt/ß-catenin pathway and by changing endogenous metabolites.


Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Dendrobium/química , Regulación Neoplásica de la Expresión Génica , Polisacáridos/farmacología , Lesiones Precancerosas/prevención & control , Neoplasias Gástricas/prevención & control , Animales , Antineoplásicos Fitogénicos/aislamiento & purificación , Betaína/sangre , Ciclina D1/genética , Ciclina D1/metabolismo , Glucógeno Sintasa Quinasa 3 beta/genética , Glucógeno Sintasa Quinasa 3 beta/metabolismo , Masculino , Metaboloma/genética , Metilnitronitrosoguanidina/toxicidad , Extractos Vegetales/química , Polisacáridos/aislamiento & purificación , Lesiones Precancerosas/inducido químicamente , Lesiones Precancerosas/genética , Lesiones Precancerosas/patología , Antígeno Nuclear de Célula en Proliferación/genética , Antígeno Nuclear de Célula en Proliferación/metabolismo , Ratas , Ratas Sprague-Dawley , Transducción de Señal , Neoplasias Gástricas/inducido químicamente , Neoplasias Gástricas/genética , Neoplasias Gástricas/patología , Proteínas Wnt/genética , Proteínas Wnt/metabolismo , beta Catenina/genética , beta Catenina/metabolismo
14.
Molecules ; 24(14)2019 Jul 23.
Artículo en Inglés | MEDLINE | ID: mdl-31340585

RESUMEN

The stems of Dendrobium loddigesii, a Chinese herb, are often used to treat diabetes and its polar extract is rich in shihunine, a water-soluble Orchidaceae alkaloid, but little is known about the anti-diabetes effects and mechanism of shihunine. This study investigated the anti-diabetic effect of a shihunine-rich extract of D. loddigesii (DLS) based on 3T3-L1 cells and db/db mice. The underlying mechanisms were primarily explored using Western blot analysis and immunohistochemical staining. The 3T3-L1 cell experiments showed that DLS can reduce the intracellular accumulation of oil droplets as well as triglycerides (p < 0.001) and promote the 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2deoxyglucose (2-NBDG) uptake of 3T3-L1 cells (p < 0.001). The animal experiments confirmed that after 8 weeks of DLS treatment, the body weight, fasting blood sugar, and serum lipid levels of mice were significantly lowered, and the oral glucose tolerance test and serum insulin level were significantly improved compared to the no-treatment diabetes mellitus group. Further histomorphology observation led to the conclusion that the quantities of islet cells were significantly increased and the increase in adipose cell size was significantly suppressed. The immunohistochemical test of pancreatic tissue revealed that DLS inhibited the expression of cleaved cysteine aspartic acid-specific protease 3 (cleaved caspase-3). Western blot experiments showed that DLS had agonistic effects on adenosine monophosphate (AMP)-activated protein kinase phosphorylation (p-AMPK) and increased the expression levels of peroxisome proliferator-activated receptor α (PPARα) and glucose transporter 4 (GLUT4) in liver or adipose tissues. These data suggest that the shihunine-rich extract of D. loddigesii is an anti-diabetic fraction of D. loddigesii. Under our experimental condition, DLS at a dose of 50 mg/kg has good anti-diabetic efficacy.


Asunto(s)
Glucemia/efectos de los fármacos , Dendrobium/química , Diabetes Mellitus Experimental/tratamiento farmacológico , Hipoglucemiantes/farmacología , Lactonas/farmacología , Extractos Vegetales/farmacología , Pirrolidinas/farmacología , Células 3T3-L1 , 4-Cloro-7-nitrobenzofurazano/análogos & derivados , 4-Cloro-7-nitrobenzofurazano/metabolismo , Proteínas Quinasas Activadas por AMP/genética , Proteínas Quinasas Activadas por AMP/metabolismo , Animales , Transporte Biológico , Glucemia/metabolismo , Caspasa 3/genética , Caspasa 3/metabolismo , Desoxiglucosa/análogos & derivados , Desoxiglucosa/metabolismo , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/fisiopatología , Ayuno , Regulación de la Expresión Génica , Prueba de Tolerancia a la Glucosa , Transportador de Glucosa de Tipo 4/genética , Transportador de Glucosa de Tipo 4/metabolismo , Hipoglucemiantes/aislamiento & purificación , Lactonas/aislamiento & purificación , Gotas Lipídicas/química , Gotas Lipídicas/efectos de los fármacos , Masculino , Ratones , Ratones Transgénicos , PPAR alfa/genética , PPAR alfa/metabolismo , Extractos Vegetales/química , Tallos de la Planta/química , Pirrolidinas/aislamiento & purificación , Transducción de Señal , Triglicéridos/antagonistas & inhibidores , Triglicéridos/metabolismo
15.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2600-2606, 2019 Jun.
Artículo en Chino | MEDLINE | ID: mdl-31359730

RESUMEN

Books on Chinese herbal medicines have shown that Dendrobium has the effect of nourishing Yin and reinforcing Yin,usually used for constipation induced by spleen Yin deficiency in clinical application. D. huoshanense,as an independent species among many species of Dendrobium,has no experimental studies about its effects on spleen Yin deficiency-type constipation. The purpose of this experiment was to illustrate the therapeutic effect of D. huoshanense on the constipation of spleen Yin deficiency type in rats,investigate its preliminary mechanism,and compare it with the D. officinale and D. nobile contained in the Chinese Pharmacopoeia to clarify its characteristics. The spleen Yin deficiency model was replicated in 70 rats by the composite factor method,and then the model rats were randomly divided into 7 groups: model group,Liuwei Dihuang Pills group( LWDHP),D. huoshanense high( DHS-H),medium( DHS-M),low( DHS-L) dose groups,D. nobile group( DNS),and D. officinale group( DOS),and another 10 rats were used as normal group( Normal). After 7 continuous days of administration,the fecal water content and intestine propulsion rate of each group were detected. HE staining was used to observe the pathological damage of ileum and colon in each group. Immunohistochemistry and Western blot were used to detect aquaporin 3( AQP3) expressions,while the expression levels of the somatostatin( SS) and motilin( MTL) in the ileum of each group were detected by enzyme-linked immunosorbent assay. The results showed that as compared with the model group,the rats in each drug-administered group had increased number of fecal pellets,increased fecal water content,and the increased intestinal propulsion rate( P<0. 01),while the pathological damage of the ileum and colon was significantly reduced; the expression of AQP3 protein was significantly decreased( P<0. 01); the level of MTL was significantly increased and the level of SS was decreased( P<0. 01). All DHS groups showed a good dose-effect relationship,and the same dose treatment effect was equivalent to that of DOS,but it was superior to DNS. Therefore,DHS has a significant therapeutic effect on constipation of spleen Yin deficiency type,and its mechanism may be related to intestinal motility and water-liquid metabolism,with a good therapeutic effect.


Asunto(s)
Estreñimiento/tratamiento farmacológico , Dendrobium/química , Medicamentos Herbarios Chinos/farmacología , Plantas Medicinales/química , Deficiencia Yin/tratamiento farmacológico , Animales , Intestinos , Distribución Aleatoria , Ratas , Bazo
16.
Int J Mol Sci ; 20(11)2019 Jun 03.
Artículo en Inglés | MEDLINE | ID: mdl-31163611

RESUMEN

Time to flower, a process either referring to juvenile-adult phase change or vegetative-reproductive transition, is strictly controlled by an intricate regulatory network involving at least both FT/TFL1 and the micro RNA (miR)156-regulated SPL family members. Despite substantial progresses recently achieved in Arabidopsis and other plant species, information regarding the involvement of these genes during orchid development and flowering competence is still limited. Dendrobium catenatum, a popular orchid species, exhibits a juvenile phase of at least three years. Here, through whole-genome mining and whole-family expression profiling, we analyzed the homologous genes of FT/TFL1, miR156, and SPL with special reference to the developmental stages. The FT/TFL1 family contains nine members; among them, DcHd3b transcribes abundantly in young and juvenile tissues but not in adult, contrasting with the low levels of others. We also found that mature miR156, encoded by a single locus, accumulated in large quantity in protocorms and declined by seedling development, coincident with an increase in transcripts of three of its targeted SPL members, namely DcSPL14, DcSPL7, and DcSPL18. Moreover, among the seven predicted miR156-targeted SPLs, only DcSPL3 was significantly expressed in adult plants and was associated with plant maturation. Our results might suggest that the juvenile phase change or maturation in this orchid plant likely involves both the repressive action of a TFL1-like pathway and the promotive effect from an SPL3-mediated mechanism.


Asunto(s)
Proteínas de Unión al ADN/genética , Dendrobium/genética , Regulación de la Expresión Génica de las Plantas , MicroARNs/genética , Desarrollo de la Planta/genética , Proteínas de Plantas/genética , Regiones no Traducidas 3' , Secuencia de Aminoácidos , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/metabolismo , Dendrobium/clasificación , Familia de Multigenes , Fenotipo , Filogenia , Proteínas de Plantas/química , Plantas Modificadas Genéticamente , Análisis de Secuencia de ADN
17.
Chin J Nat Med ; 17(5): 337-345, 2019 May 20.
Artículo en Inglés | MEDLINE | ID: mdl-31171268

RESUMEN

Dendrobium officinale is not only an ornamental plant, but also a valuable medicinal herb that is widely used in traditional Chinese medicine. However, distinguishing D. officinale from other Dendrobium species is usually a difficult task. In this study, we developed a rapid identification protocol for D. officinale using the loop-mediated isothermal amplification (LAMP) method. A set of primers were specifically designed to detect a modified internal transcribed spacer region of D. officinale at 65 °C within 40 min after adding SYBR® Green I, which was used for the detection of D. officinale. Unlike commonly used adulterants, reaction mixtures containing D. officinale DNA changed from orange to green, and this color change was easily observed with the naked eye. Thus, this methodology can be used to accurately differentiate D. officinale from other Dendrobium species, is quick as all D. officinale samples were amplified within 40 min, and specific as samples of the adulterants were not amplified. The specificity of this LAMP-based method was confirmed by testing 17 samples of D. officinale and 32 adulterant samples from other Dendrobium species. This LAMP-based rapid identification method does not require expensive equipment or specialized techniques and can be used in field surveys for accurate and fast on-site identification.


Asunto(s)
Dendrobium/genética , Técnicas de Amplificación de Ácido Nucleico , Plantas Medicinales/genética , ADN de Plantas/genética , ADN Espaciador Ribosómico/genética , Dendrobium/clasificación , Medicina China Tradicional , Plantas Medicinales/clasificación , Especificidad de la Especie
18.
Int J Biol Macromol ; 135: 1034-1042, 2019 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-31173832

RESUMEN

In this study, a novel lactic acid probiotic named Bacillus sp. DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated. Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1). After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92 × 105 Da (UDP-1) to 5.21 × 105 Da. Fermentation increased the proportions of mannose in FDP-1 by 51.38% compared with that in UDP-1. FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1ß (IL-1ß) production in RAW 264.7 cells. Probiotic fermentation by Bacillus sp. DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp. DU-106-fermented D. officinale polysaccharides as auxiliary functional material in immunotherapy.


Asunto(s)
Bacillus/metabolismo , Dendrobium/química , Fermentación , Polisacáridos/metabolismo , Animales , Biomarcadores , Supervivencia Celular/efectos de los fármacos , Fenómenos Químicos , Interleucina-1beta/biosíntesis , Ratones , Estructura Molecular , Fagocitosis , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Células RAW 264.7 , Análisis Espectral
19.
BMC Plant Biol ; 19(1): 245, 2019 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-31182022

RESUMEN

BACKGROUND: Studies have demonstrated that BBX (B-BOX) genes play crucial roles in regulatory networks controlling plant growth, developmental processes and stress response. Nevertheless, comprehensive study of BBX genes in orchids (Orchidaceae) is not well studied. The newly released genome sequences of Dendrobium officinale and Phalaenopsis equestris have allowed a systematic analysis of these important BBX genes in orchids. RESULTS: Here we identified 19 (DoBBX01-19) and 16 (PeBBX01-16) BBX genes from D. officinale and P. equestris, respectively, and clustered into five clades (I-V) according to phylogenetic analysis. Thirteen orthologous, two DoBBXs paralogous and two PeBBXs paralogous gene pairs were validated. This gene family mainly underwent purifying selection, but five domains experienced positive selection during evolution. Noteworthy, the expression patterns of root, root_tips, stem, leaf, speal, column, lip, and flower_buds revealed that they might contribution to the formation of these tissues. According to the cis-regulatory elements analysis of BBX genes, qRT-PCR experiments were carried out using D. officinale PLBs (protocorm-like bodies) and displayed that these BBX genes were differentially regulated under AgNO3, MeJA (Methyl Jasmonate), ABA (abscisic acid) and SA (salicylic acid) treatments. CONCLUSIONS: Our analysis exposed that DoBBX genes play significant roles in plant growth and development, and response to different environmental stress conditions of D. officinale, which provide aid in the selection of appropriate candidate genes for further functional characterization of BBX genes in plants.


Asunto(s)
Dendrobium/genética , Reguladores del Crecimiento de las Plantas , Proteínas de Plantas/genética , Factores de Transcripción/genética , Transcriptoma , Ácido Abscísico/administración & dosificación , Acetatos/administración & dosificación , Secuencia de Aminoácidos , Ciclopentanos/administración & dosificación , Dendrobium/efectos de los fármacos , Perfilación de la Expresión Génica , Familia de Multigenes/efectos de los fármacos , Oxilipinas/administración & dosificación , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Ácido Salicílico/administración & dosificación , Nitrato de Plata/administración & dosificación , Factores de Transcripción/química , Factores de Transcripción/metabolismo
20.
Biomed Pharmacother ; 117: 109073, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31212129

RESUMEN

Dendrobium nobile Lindl. alkaloids (DNLA), the active ingredients of Dendrobium, has been shown to possess anti-oxidative effects. The nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant signaling pathway plays a critical role in the cellular response to oxidative stress. Oxidative damage has been implicated in the mechanism of various hepatotoxins induced liver injury. The present study aimed to examine the protective effects of DNLA on CCl4-induced acute liver injury, and to explore the role of the Nrf2 pathway in the protective action of DNLA. Wild-type (WT) and Nrf2-knockout (Nrf2-/-) mice were administrated with DNLA (20 mg/kg/day, ig) for 7 days, and then challenged with CCl4 (20 µL/kg, ip). In WT mice, DNLA reduced CCl4 induced liver injury, as evidenced by the reduction in the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), attenuation of malondialdehyde (MDA) production, and improved ultrastructural morphology in hepatocytes. However, the protective effect was diminished in Nrf2-/- mice, indicating an essential role of Nrf2 in DNLA-mediated protection over CCl4 liver injury. Furthermore, it was found that DNLA enhanced Nrf2 expression and nuclear accumulation and increased the expression of Nrf2 regulated downstream proteins. These results demonstrate that DNLA protects mice from CCl4 induced liver injury, probably through the activation of the Nrf2 signaling pathway.


Asunto(s)
Alcaloides/farmacología , Dendrobium/química , Hígado/lesiones , Factor 2 Relacionado con NF-E2/metabolismo , Sustancias Protectoras/farmacología , Transducción de Señal , Enfermedad Aguda , Animales , Tetracloruro de Carbono , Hígado/efectos de los fármacos , Hígado/patología , Hígado/ultraestructura , Masculino , Malondialdehído/metabolismo , Ratones Endogámicos C57BL , Factor 2 Relacionado con NF-E2/deficiencia
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