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2.
Oral Health Prev Dent ; 18(4): 713-718, 2020 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-32895654

RESUMEN

PURPOSE: To investigate dentin abrasivity and cleaning efficacy of novel/alternative toothpastes containing diamond particles, active carbon, sea salt or organic oils. MATERIALS AND METHODS: Seventy-two bovine dentin samples (for measuring abrasivity) and 60 human dentin samples (for assessing cleaning efficacy) were used in this study. Samples were divided into six groups as follows: group 1: Elmex Kariesschutz (hydrated silica); group 2: Lavera Neutral Zahngel (sea salt); group 3: Curaprox Black is White (active carbon); group 4: Swiss Smile Diamond Glow (diamond powder); group 5: Ringana Fresh Tooth Oil (hydrated silica); and group 6: artificial saliva. Samples were brushed for a total of 26 min at 120 strokes/min, replacing slurries (1 part respective toothpaste and 2 parts artificial saliva) every 2 min. Finally, abrasive dentin wear was measured profilometrically and cleaning efficacy planimetrically. RESULTS: The highest abrasivity values were observed for Lavera Neutral Zahngel (sea salt 9.2 µm) and Elmex Kariesschutz group (hydrated silica 6.0 µm). The lowest abrasivity value was observed for Ringana Fresh Tooth Oil group (hydrated silica 1.3 µm). The highest cleaning efficacy was observed for Elmex Kariesschutz group (86.7%) and the lowest cleaning efficacy was observed for Ringana Fresh Tooth Oil group (31.3%). CONCLUSION: The addition of diamond powder or active carbon to toothpastes could offer high cleaning efficacy with low dentin abrasivity. The addition of sea salt to traditional abrasives might cause high abrasive dentin wear without adding further cleaning benefit.


Asunto(s)
Abrasión de los Dientes , Pastas de Dientes , Animales , Bovinos , Dentina , Humanos , Saliva Artificial , Cepillado Dental
3.
Oral Health Prev Dent ; 18(4): 807-814, 2020 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-32895665

RESUMEN

PURPOSE: To investigate whether toothpastes with diamond powder vs those with traditional abrasives abrade dentin and enamel differently and to determine the relative dentin abrasivity (RDA) and relative enamel abrasivity (REA) values of those toothpastes. MATERIALS AND METHODS: Dentin and enamel samples of bovine permanent incisors were randomly allocated into groups of eight, brushed with 20 different toothpastes (three of which contained diamond powder) and analysed for their RDA and REA values. RESULTS: Toothpastes with diamond powder exhibit low RDA values but high REA values. Some RDA values exceeded the ones declared by the manufacturer. CONCLUSION: Diamond powder as an abrasive might have a mild action on dentin, but it is highly abrasive on enamel.


Asunto(s)
Abrasión de los Dientes , Pastas de Dientes , Animales , Bovinos , Esmalte Dental , Dentina , Diamante , Cepillado Dental
4.
Braz Dent J ; 31(4): 409-416, 2020 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-32901718

RESUMEN

This study was conducted to evaluate whether antibacterial pretreatment irrigation with silver nanoparticles (SNPs) and silver diamine fluoride (SDF) has any effect on bond strength of fiber posts cemented with three types of resin cements in root canal space. Fifty-four endodontically treated maxillary central incisor roots were prepared for fiber post (FRC Postec Plus NO.3, Ivoclar Vivadent) cementation and divided into nine groups in terms of three cement types and two pretreatments with silver antibacterial agents. The cements were as follows: an etch-and-rinse cement (ER, Excite DSC/Variolink N), a self-etch cement (SE, ED Primer/Panavia F2.0), and a self-adhesive cement (SA, Panavia SA Luting Plus). For each cement, the control group was with no treatment and two experimental groups were with SNPs and SDF treatments that were used after acid-etching for ER cement and after EDTA treatment for SE and SA cements. After fiber post cementation, each bonded root was horizontally sectioned into 1-mm thickness microslices to create two slices for each root region (apical, middle and coronal) and underwent push-out bond strength (PBS) test. Data in MPa were analyzed with two-way ANOVA and Tukey test (p=0.05). The interaction of the pretreatment type and cement type was significant (p<0.001). SNPs and SDF significantly increased PBS with ER cement (p≤0.04). This positive effect was also marginally significant for SDF with SE cement (p=0.049). For SA cement, SNPs showed a significant positive effect, but SDF had a significant adverse effect on PBS (p<0.001). The effect of pretreatment with silver antibacterial agents prior to adhesive cementation of fiber posts depends on the resin cement used. Contrary to SNPs with beneficial or no significant effect on bonding for all cements, SDF exhibited a deleterious effect with self-adhesive cement.


Asunto(s)
Recubrimiento Dental Adhesivo , Nanopartículas del Metal , Técnica de Perno Muñón , Antibacterianos , Dentina , Plata
5.
Ned Tijdschr Tandheelkd ; 127(7-08): 424-433, 2020.
Artículo en Holandés | MEDLINE | ID: mdl-32840498

RESUMEN

The International Caries Consensus Collaboration (ICCC) presented recommendations on terminology, methods of carious tissue removal and managing cavitated carious lesions. It identified 'dental caries' as the disease that dentists should manage by controlling the activity of existing cavitated lesions by preserving as much hard tissue as possible, maintaining pulp sensibility and retaining functional teeth in the long-term. The ICCC recommended the level of hardness as the criterion for determining the clinical consequences of the process of demineralisation and defined new strategies for the selective removal of carious tissue. The starting point is to effectively remove the biofilm from cavitated carious lesions. Only when cavitated carious lesions are either non-cleansable or can no longer be sealed, are restorative interventions indicated, with due regard for the principles of a minimally invasive approach. Applying a restoration facilitates biofilm removal, guards the pulpodental complex and restores form, function and aesthetics.


Asunto(s)
Caries Dental , Biopelículas , Consenso , Dentina , Humanos
6.
Ned Tijdschr Tandheelkd ; 127(7-08): 417-423, 2020.
Artículo en Holandés | MEDLINE | ID: mdl-32840497

RESUMEN

This in vitro study investigated whether aging of restorative materials influences secondary caries development. The research question was whether antibacterial bonding maintained its effectiveness after aging. 60 enamel-dentine blocks were restored using 3 different restorative materials (n = 20 per material): composite with conventional bonding, composite with antibacterial bonding and amalgam. Half of the samples in each group (n = 10) were artificially aged through exposure to a sucrose biofilm model. Lesion depth was subsequently measured using transversal microradiography. Aging led to deeper lesions in the composite with antibacterial bonding group (CAB), compared to fresh samples. These fresh samples showed lesions less deep than fresh samples of composite with conventional bonding. After aging, this effect was absent. Within the limitations of this short-term in vitro study, it can be concluded that aging of restorative materials can influence caries progression. Antibacterial bonding materials lose their effectiveness over time.


Asunto(s)
Caries Dental , Recubrimientos Dentinarios , Envejecimiento , Antibacterianos , Resinas Compuestas , Restauración Dental Permanente , Dentina , Humanos
7.
Int J Nanomedicine ; 15: 4755-4762, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32753864

RESUMEN

Aim: This study was conducted to evaluate the effects of three nanoparticle solutions used as dentin pretreatments on the microshear bond strength (µSBS) of a conventional glass ionomer cement (GIC) to dentin. Materials and Methods: Ninety intact human molars were used after sectioning their occlusal surfaces to expose flat dentin surfaces. The specimens were randomly assigned to nine groups (n = 10). Group A was the control group (without using the cavity disinfectant). In groups B, C, D, and E, the prepared dentin surfaces were treated with 1 cc 2% chlorhexidine (CHX), 0.1% silver nanoparticle (SNP), 0.1% titanium dioxide nanoparticle (TNP), and 0.1% zinc oxide nanoparticle (ZNP) solutions for 1 minute, respectively, before applying the conditioner. CHX, SNPs, TNPs, and ZNPs were applied for 1 minute after applying the conditioner in groups F, G, H, and I, respectively. The specimens were restored with a conventional GIC and underwent µSBS testing after 24 hours. The data were analyzed using the one-way analysis of variance and Tukey's test (p=0.05). Results: The applications of the nanoparticles (SNP, TNP, and ZNP) after the conditioner were associated with significantly greater µSBS values compared to that of the control group (p values < 0.05). Significantly higher µSBS values were observed when TNP or ZNP was applied after the conditioner compared to their applications before the conditioner (p values < 0.05). The highest µSBS values were observed when TNP was applied after the conditioner. Conclusion: Dentin pretreatment with the nanoparticles after applying the conditioner enhanced the bond strength of the GIC to dentin compared with the control group. The best results were obtained for the TNPs applied after the conditioner.


Asunto(s)
Recubrimiento Dental Adhesivo , Dentina/efectos de los fármacos , Cementos de Ionómero Vitreo/farmacología , Nanopartículas del Metal/química , Plata/farmacología , Titanio/farmacología , Óxido de Zinc/farmacología , Clorhexidina/farmacología , Cementos de Ionómero Vitreo/química , Humanos , Ensayo de Materiales , Propiedades de Superficie
8.
J Dent Child (Chic) ; 87(2): 69-76, 2020 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-32787999

RESUMEN

Purpose: To evaluate the morphological alterations in enamel and dentin of primary teeth following radiotherapy (RT) and to determine the best adhesive technique and time to carry out restorative procedures.
Methods: Enamel and dentin fragments of primary teeth were randomly assigned into four groups (n=30): G1 (control)-non-irradiated, only restorative procedure; G2- restorative procedure immediately before RT; G3-restorative procedure 24 hours after RT; and G4-restorative procedure six months after RT. Each group was divided into one of two subgroups according to the adhesive system used for restoration: (1) AdperSingle Bond 2 (SB); and (2) ClearfillSE Bond (CL). The specimens were submitted to fractionated RT until they reached the final dose of 60 Gy. They were then subjected to confocal microscopy and the shear bond strength test. Data were analyzed using two-way analysis of variance followed by Tukey's tests ( α = five percent).
Results: Morphological changes were first observed in enamel and dentin after 40 Gy of irradiation. G4 bond strength values were similar to G1 in the CL and SB groups for enamel and in the CL group for dentin (P >0.05). G2 showed the lowest values for enamel and dentin (P <0.05). In G3, CL presented the highest strength values in enamel; for G4, the highest values were found in dentin (P <0.05).
Conclusions: Radiotherapy affected the morphological surface of enamel and dentin. The restorations placed immediately after RT had the weakest shear bond strength, and the restorations placed six months after RT had similar means of bond strength compared to the nonirradiated teeth in enamel, regardless of the adhesive system used. In dentin, CL showed better performance than SB.


Asunto(s)
Recubrimiento Dental Adhesivo , Recubrimientos Dentinarios , Resinas Compuestas , Cementos Dentales , Dentina , Humanos , Ensayo de Materiales , Cementos de Resina , Resistencia al Corte , Diente Primario
9.
J Dent Child (Chic) ; 87(2): 83-89, 2020 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-32788001

RESUMEN

Purpose: Radiation-related caries is characterized by enamel delamination near the dentinoenamel junction (DEJ). We investigated the activity and expression of the matrix metalloproteinases (MMPs) -2 and -9 in order to understand disease pathogenesis in teeth submitted or not to radiotherapy (RT).
Methods: In situ zymography and immunofluorescence assays were performed to evaluate the activity and expression of MMPs -2 and -9, respectively. Twelve primary second molars were randomly assigned into two experimental subgroups: irradiated and nonirradiated. Dental fragments were exposed to radiation at a dose fraction of two Gy for five consecutive days until reaching the total dose of 60 Gy. The percentage of fluorescence in the DEJ was evaluated in three distinct regions of the tooth (cervical, cusp, and pit). The regions were photographed under fluorescence microscopy at 1.25× and 5× magnification.
Results: The intensity of fluorescence per mm 2 in the DEJ was higher in the cervical region of irradiated primary teeth (P <0.05) versus nonirradiated ones. In these areas, immunofluorescence revealed expression of MMPs -2 and -9.
Conclusion: Radiotherapy can increase the activity of MMPs -2 and -9 in the cervical region of the DEJ of primary teeth.


Asunto(s)
Dentina , Metaloproteinasas de la Matriz , Esmalte Dental , Humanos , Diente Molar , Diente Primario
10.
Am J Dent ; 33(4): 196-200, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32794394

RESUMEN

PURPOSE: To evaluate the effect of silver diamine fluoride (SDF) application on the microshear bond strength (MBS) of glass-ionomer cements (GIC) to caries-affected dentin (Part 1) and dentin cleaning methods to reduce SDF's potential effect on MBS (Part 2). METHODS: For Part 1, 56 extracted human teeth were randomly divided into eight groups with GIC, 38% SDF application and dentin substrate. Samples of artificial caries-affected human dentin were treated or not with 38% SDF and restored with conventional or resin-modified GIC. The same procedures were performed in sound dentin tested for MBS test after 24 hours. In Part 2, different dentin cleaning agents (water, aluminum oxide, and pumice slurry) were tested after SDF application. The procedure was performed on the group that presented the worst values for MBS in Part 1. Fracture mode was evaluated under scanning electron microscope. Data were statistically analyzed by ANOVA. RESULTS: MBS was affected by the presence of caries and the type of material, with the conventional GIC the most affected (P< 0.05). Pumice slurry was superior in comparison to the other agents in cleaning SDF-treated dentin. Fracture evaluation showed more mixed failures in all the groups. CLINICAL SIGNIFICANCE: Clinicians should have caution when selecting the glass-ionomer cement (GIC) for restorations in silver diamine fluoride (SDF)-treated dentin. The mechanical properties of conventional GIC restorations were more affected than resin-modified GICs. Pumice slurry was the most effective cleaning method to minimize the negative effect of SDF on dentin.


Asunto(s)
Recubrimiento Dental Adhesivo , Cementos de Ionómero Vitreo , Dentina , Fluoruros Tópicos , Humanos , Ensayo de Materiales , Compuestos de Amonio Cuaternario , Cementos de Resina , Compuestos de Plata
11.
Am J Dent ; 33(4): 206-212, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32794396

RESUMEN

PURPOSE: This double-blind, randomized clinical trial evaluated the influence of dentin moisture on postoperative sensitivity (POS) in posterior restorations using a simplified etch-and-rinse adhesive, until 12 months of clinical service. METHODS: 90 restorations were inserted in 45 patients to treat carious lesions or to replace existing posterior restorations with a depth ≥ 3 mm. After cavity preparation, the simplified etch-and-rinse adhesive (Adper Single Bond 2) was applied on dry or wet dentin followed by a bulk-fill resin composite (Filtek Bulk Fill) under rubber dam isolation. The patient's spontaneous and stimulated POS was evaluated at baseline and after 7 days, 6 months, and 12 months of clinical evaluation. The secondary parameters (marginal discoloration, marginal adaptation, fracture and recurrence of caries) were evaluated by World Dental Federation (FDI) criteria after 7 days, 6 and 12 months of clinical evaluation. RESULTS: No significant spontaneous and stimulated POS was observed when dry and wet dentin were compared (P> 0.05). A significant and higher risk of spontaneous POS (18.6%; 95% CI 9.7 to 32.6) occurred up to 48 hours after restoration placement for both groups when compared to all evaluation times (P< 0.03). However, the intensity of POS was mild at up to 48 hours with a difference between the dry and wet dentin groups (P> 0.79). When secondary parameters were evaluated, no significant difference between the groups were observed (P> 0.05). CLINICAL SIGNIFICANCE: The moisture level of the dentin substrate in posterior restorations does not influence POS in bulk-fill resin composite posterior restorations when associated with an etch-and-rinse ethanol-based adhesive system.


Asunto(s)
Caries Dental , Sensibilidad de la Dentina , Resinas Compuestas , Adaptación Marginal Dental , Restauración Dental Permanente , Dentina , Recubrimientos Dentinarios , Método Doble Ciego , Humanos , Cementos de Resina
12.
Braz Oral Res ; 34: e081, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32696905

RESUMEN

The aim of this study was to investigate the influence of shortening of etching time for dentin on the restoration survival after selective carious tissue removal in primary molars. This two-arm randomized clinical trial included sixty-two subjects (5-8 year-old) and 100 primary molars presenting moderate dentin carious lesions on occlusal surface. The sample was randomly assigned into groups previously to adhesive application (Adper Single Bond 2; 3M ESPE): etching time recommended by manufacturer (15 s) or reduced (7 s). Resin composite (Filtek Bulk Fill Posterior Restorative; 3M ESPE) was inserted in a single increment for all restorations. Restorations were evaluated at 1, 6, 12, and 18 months using FDI criteria. Survival estimates for restorations' longevity were evaluated with Kaplan-Meier method. Multivariate Cox regression analysis with shared frailty was used to assess the factors associated with failures (p < 0.05). The etching time did not influence the restorations' survival (HR 0.35 95%CI 0.11-1.12; p = 0.06). Mean estimated time of survival was 17.6 months (95%CI, 17.2-17.9). The survival rates at the 18-month follow-up were 75.7% and 91.4% (AFR: 16.9% and 5.7%) when primary dentin was acid etched for 15 and 7 s, respectively (log-rank p = 0.06). In conclusion, the etching time for dentin did not influence the clinical behavior of adhesives restorations. However, there was a tendency for better clinical outcome when using etching time of 7 s.


Asunto(s)
Diente Primario , Resinas Compuestas , Restauración Dental Permanente , Dentina , Recubrimientos Dentinarios , Diente Molar
13.
J Endod ; 46(9): 1302-1308, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32615175

RESUMEN

INTRODUCTION: This study aimed to evaluate the effect of ultrasonic activation (UA) of endodontic sealers on dentin tubule penetration and the bond strength to root dentin. METHOD: One hundred single-rooted teeth were prepared with 40.06 nickel-titanium instruments and divided into 2 groups: with or without UA. Three resin-based sealers (MTA Fillapex [Angelus Dental Solutions, Londrina, PR, Brazil], Sealer Plus [MK Life Medical and Dental Products, Porto Alegre, RS, Brazil], and AH Plus [Dentsply, DeTrey GmbH, Konstanz, Germany]; n = 20) and 2 calcium silicate-based sealers (Sealer Plus BC [MK Life Medical and Dental Products] and EndoSequence BC [Brasseler, Savannah, GA], n = 20) were used and subdivided (n = 10) according to the protocols. Fluo-3 (Thermo Fisher Scientific, Waltham, MA) and rhodamine B dyes were added to the calcium silicate- and resin-based sealers, respectively. In the UA groups, the activation was performed for 40 seconds followed by lateral compaction. Samples were transversely sectioned to evaluate the dentin tubule penetration and the bond strength to root dentin. The penetration data were analyzed with the Student t test, 1-factor analysis of variance, and Bonferroni tests. Bond strength was evaluated using the Student t test, Kruskal-Wallis, and Dunn post hoc test. RESULTS: Resin-based sealers showed the highest tubule penetration without UA (P < .05). UA significantly enhanced MTA Fillapex and Endosequence BC dentin tubule penetration (P < .05). AH Plus and Sealer Plus BC improved their bond strength to root dentin after UA (P < .05). AH Plus/UA, Sealer Plus/UA, and Sealer Plus BC/UA presented the highest bond strength values (P < .05). Adhesive failures were predominant in all groups regardless of the use of ultrasound. CONCLUSIONS: UA interferes with tubule penetration and the bond strength to root dentin of resin- and calcium silicate-based sealers.


Asunto(s)
Recubrimiento Dental Adhesivo , Materiales de Obturación del Conducto Radicular , Brasil , Compuestos de Calcio , Dentina , Resinas Epoxi , Alemania , Humanos , Ensayo de Materiales , Silicatos , Raíz del Diente
15.
Braz Oral Res ; 34: e064, 2020 Jun 24.
Artículo en Inglés | MEDLINE | ID: mdl-32609233

RESUMEN

This study evaluated the interference of enamel and coronal dentin thickness and ambient light in pulse oximetry interpretation, using SaO2the human finger as a reference. Forty-two intact human permanent mandibular molars were sectioned mesiodistally in the central portion. Buccal and lingual dentin surfaces were drilled, and 4 mm, 3 mm and 2 mm enamel/dentin thicknesses were interposed between finger and device, after which SaO2 levels were measured by finger pulse oximetry. A reference device was designed to align pulse oximeter light-emitting diode, buccal surface of tooth, little finger, lingual surface of tooth and photodiode. Variables were described as means and standard deviation, and the confidence interval was reported. Repeated measures analysis of variance (ANOVA) was used to compare the groups, followed by Bonferroni correction. Student t test for paired samples was used to determine presence of ambient light (α = 0.05). Mean SaO2 level was lower at 4.0 ± 0.2 mm thickness, regardless of presence or absence of ambient light (92.7% and 89.3%). The other thicknesses yielded values of 95.5% and 94.5% at 3.0 ± 0.2 mm, and 96.4% and 96.0% at 2.0 ± 0.2 mm (p < 0.001). There were significant differences between SaO2 values at 4.0 ± 0.2 mm, 3.0 ± 0.2 mm and 2.0 ± 0.2 mm thicknesses, in the presence of ambient light. Mean SaO2 level in positive control was 96.3%, and mean pulse rate was 69.5 bpm in presence of ambient light; in the absence of light, these values were 96% and 70.5 bpm. Enamel and dentin thickness interfere with SaO2, regardless of presence or absence of ambient light. The lowest SaO2 levels were found for the thickest tooth samples.


Asunto(s)
Esmalte Dental , Oximetría , Dentina , Humanos , Diente Molar , Oxígeno
16.
Niger J Clin Pract ; 23(7): 950-956, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32620724

RESUMEN

Background: During post restoration, different root structures require several types of posts to increase duration of their clinical use. Several materials have been investigated to enhance their quality and optimize their length according to the available root canal. Aims: The aim of this study was to determine the effect of zirconia, fiber, and ceromer posts with 3- and 6-mm post sizes on the bonding strength of them to root canal dentinal wall with the means of pull-out bond strength test. Methods: Forty-eight single-rooted mandibular human premolar teeth were collected and prepared for this in vitro study. With resin cement, 3- and 6-mm study posts including zirconia, fiber, and ceromer were luted to prepare teeth. For the retention testing, the pull-out force was applied to each specimen parallel to longitudinal axis of both the post and tooth. Results: Both type of materials and size of posts changed the value of bonding strength. In all the post types, 6-mm ones performed better. Overall, the best bonding strength was obtained with fiber posts and the better bonding strength was obtained with zirconia; however, ceromer provided the least bonding strength. Conclusion: Current experiments supported that 6-mm post size can increase the bonding between root canal dentin and studied posts. When considering post materials, fiber provided the best bonding strength in current laboratory setup. Second, zirconia had meaningfully acceptable bonding strength; however, the bonding strength of ceromer posts was not favorable. Further studies optimizing post fabrication techniques of root materials may increase the bonding strength of posts to human dentin to an acceptable clinical degree.


Asunto(s)
Recubrimiento Dental Adhesivo , Cavidad Pulpar , Dentina , Técnica de Perno Muñón , Cementos de Resina/química , Tratamiento del Conducto Radicular/métodos , Raíz del Diente/cirugía , Cerámica , Resinas Compuestas , Vidrio/química , Humanos , Ensayo de Materiales , Circonio
17.
PLoS One ; 15(7): e0235080, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32639959

RESUMEN

Detailed information about the lives and deaths of children in antiquity is often in short supply. Childhood dietary histories are, however, recorded and maintained in the teeth of both juveniles and adults. Primary tooth dentinal collagen does not turn over, preserving a sequential record of dietary changes. The use of nitrogen (δ15N) and carbon (δ13C) isotope values of incrementally sampled dentin are used in the study of breastfeeding practices but evidence for the addition of weaning foods, both in terms of mode and, particularly, duration, has remained analytically inaccessible to date. Here, we demonstrate how the novel use hydrogen isotope (δ2H) values of sequentially micro-sampled dentin collagen, measured from individuals excavated from a Punic cemetery, in Sardinia, Italy, can serve as a proxy for weaning food type and duration in ancient childhood diet. The weaning rate and age, based on the decline in δ15N and δ13C values of permanent first molars and the concomitant increase in δ2H, appears to be broadly similar among six individuals. Hydrogen isotopes vary systematically from a low value soon after birth, rising through early childhood. The early post-birth values can be explained by the influence of 2H-depleted lipids from mother's breastmilk and the later δ2H rise is consistent with, among other things, a substantial portion of boiled foodstuffs, such as the higher δ2H values observed in porridge. Overall δ2H in dentin shows great promise to elucidate infant and childhood feeding practices, and especially the introduction of supplementary foods during the weaning process.


Asunto(s)
Dieta/historia , Lactancia Materna/historia , Preescolar , Colágeno/análisis , Dentina/química , Deuterio/análisis , Femenino , Historia Antigua , Humanos , Lactante , Italia , Destete
18.
PLoS One ; 15(7): e0235283, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32614918

RESUMEN

Recurrent caries, the development of carious lesions at the interface between the restorative material and the tooth structure, is highly prevalent and represents the primary cause for failure of dental restorations. Correspondingly, we exploited the self-assembly and strong antibiofilm activity of amphipathic antimicrobial peptides (AAMPs) to form novel coatings on dentin that aimed to prevent recurrent caries at susceptible cavosurface margins. AAMPs are alternative to traditional antimicrobial agents and antibiotics with the ability to target the complex and heterogeneous organization of microbial communities. Unlike approaches that have focused on using these AAMPs in aqueous solutions for a transient activity, here we assess the effects on microcosm biofilms of a long-acting AAMPs-based antibiofilm coating to protect the tooth-composite interface. Genomewise, we studied the impact of AAMPs coatings on the dental plaque microbial community. We found that non-native all D-amino acids AAMPs coatings induced a marked shift in the plaque community and selectively targeted three primary acidogenic colonizers, including the most common taxa around Class II composite restorations. Accordingly, we investigated the translational potential of our antibiofilm dentin using multiphoton pulsed near infra-red laser for deep bioimaging to assess the impact of AAMPs-coated dentin on plaque biofilms along dentin-composite interfaces. Multiphoton enabled us to record the antibiofilm potency of AAMPs-coated dentin on plaque biofilms throughout exaggeratedly failed interfaces. In conclusion, AAMPs-coatings on dentin showed selective and long-acting antibiofilm activity against three dominant acidogenic colonizers and potential to resist recurrent caries to promote and sustain the interfacial integrity of adhesive-based interfaces.


Asunto(s)
Antiinfecciosos/administración & dosificación , Péptidos Catiónicos Antimicrobianos/administración & dosificación , Biopelículas/efectos de los fármacos , Placa Dental/tratamiento farmacológico , Antiinfecciosos/farmacología , Péptidos Catiónicos Antimicrobianos/farmacología , Biopelículas/crecimiento & desarrollo , Materiales Biocompatibles Revestidos/química , Dentina/química , Dentina/microbiología , Durapatita/química , Humanos , Proteínas Inmovilizadas/administración & dosificación , Proteínas Inmovilizadas/farmacología , Microbiota/efectos de los fármacos
19.
Acta Odontol Latinoam ; 33(1): 27-32, 2020 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-32621596

RESUMEN

The aim of this study was to assess the influence of different pre-treatment approaches on glass ionomer cement (GIC) bond strength (BS) to dentine. Sixty bovine incisors were allocated into six groups according to substrate - sound or caries-affected dentine; and substrate pre-treatment approach - no conditioning (control), polyacrylic acid for 10 s and phosphoric acid for 7 s. Teeth in the caries-affected dentine group were previously submitted to cariogenic pH-cycling challenge. After dentine pre-treatment, according to experimental groups, polyethylene tubes were placed on flat dentine surfaces and filled with GIC. Teeth were stored in distilled water for 24 h at 37 °C and then submitted to microshear test (0.5 mm/min). Failure pattern analysis was performed under stereomicroscope (400x). Data were analysed using two-way ANOVA and Tukey's test (α=5%). Statistically significant differences were found for the pre-treatment approach, regardless of substrate (p<0.001). The polyacrylic acid group and control group had similar BS values, and were both better than the phosphoric acid group. In general, GIC had better bonding performance in sound dentine than in caries-affected dentine. In conclusion, dentine pre-treatment with polyacrylic acid did not improve the performance of GIC restoration on clinically relevant substrates.


Asunto(s)
Resinas Acrílicas/farmacología , Recubrimiento Dental Adhesivo/métodos , Dentina/efectos de los fármacos , Cementos de Ionómero Vitreo/química , Animales , Bovinos , Filtración Dental/prevención & control , Dentina/diagnóstico por imagen , Dentina/patología , Ensayo de Materiales
20.
Shanghai Kou Qiang Yi Xue ; 29(2): 213-216, 2020 Apr.
Artículo en Chino | MEDLINE | ID: mdl-32626888

RESUMEN

PURPOSE: To investigate the efficacy of autologous dentin particles combined with platelet-rich fibrin membrane (PRF) in the treatment of root bifurcation lesions of mandibular first molar. METHODS: Ninety-three patients (93 teeth) with mandibular first molar root bifurcation lesions were selected from our department from February 2016 to October 2017. They were randomly divided into 2 groups. Forty-six patients with 46 teeth in the experimental group underwent autologous dentin particles combined with platelet-rich fibrin membrane, while patents in the control group (47 patients with 47 teeth) were treated with Bio-Oss implanted in the bone defect area covered with collagen membrane. The patients were revisted at 1, 3, 6 and 12 months after operation. The success rate of the operation group, the depth of periodontal pocket (PD), the loss of attachment (AL), the depth of penetration of the root bifurcation (HPD), and the bone density of the root bifurcation area before and after treatment. The data were recorded and compared with SPSS25.0 software package. RESULTS: The success rate was 97.83%(45/46) in the experimental group, 85.11%(40/47) in the control group, the difference between the two groups was significant(P<0.05). After treatment, PD, AL and HPD decreased significantly (P<0.05), and MGVs increased gradually. There was no significant difference in MGVs before treatment and 1 month after treatment in the experimental group (P>0.05). MGVs at other time points were significantly higher than those of the control group (P<0.05). PD, AL and HPD of the experimental group were lower significantly than the control group at each time point after treatment (P<0.05), and MGVs value was significantly higher than the control group (P<0.05). There was no significant difference in the incidence of complications(4.35% vs 6.38%, χ2=0.189, P>0.05). CONCLUSIONS: Autologous dentin particles combined with platelet-rich fibrin membrane is effective for the treatment of root bifurcation lesions of mandibular first molar, which is worthy of wide application.


Asunto(s)
Fibrina Rica en Plaquetas , Densidad Ósea , Dentina , Humanos , Diente Molar , Bolsa Periodontal
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