Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 10.918
Filtrar
1.
Pan Afr Med J ; 38: 132, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33912302

RESUMEN

Introduction: we present a rat experimental model used to evaluate the possible reduction in the extent of pancreatic tissue injury in acute pancreatitis cases, after administration of eugenol. Methods: one hundred and twenty Wistar rats were used, which were randomly assigned in 3 groups: sham (n=20), control (n=50) and eugenol (n=50). Acute pancreatitis was induced by biliopancreatic ligation in the control and eugenol groups, but not in the Sham group. In the eugenol group, eugenol was administered per-os. Five histopathological parameters, such as edema, inflammatory infiltration, duct dilatation, hemorrhage and acinar necrosis were evaluated. Results: at 72 h from acute pancreatitis induction, the total histological score was diminished in the eugenol group (p<0.0005) and duct dilatation and inflammatory infiltration were reduced compared to the control group (p<0.05). In addition, at 72 h, eugenol reduced pancreatic myeloperoxidase activity (p<0.0005). Conclusion: eugenol, a highly free radical scavenger agent, may have a preventive role in acute pancreatic injury, as it was evident in our rat experimental model.


Asunto(s)
Eugenol/farmacología , Depuradores de Radicales Libres/farmacología , Pancreatitis/prevención & control , Peroxidasa/metabolismo , Enfermedad Aguda , Animales , Modelos Animales de Enfermedad , Masculino , Páncreas/efectos de los fármacos , Páncreas/enzimología , Ratas , Ratas Wistar
2.
Molecules ; 26(6)2021 Mar 14.
Artículo en Inglés | MEDLINE | ID: mdl-33799363

RESUMEN

Quercetin is a flavonoid that is found in many plant materials, including commonly eaten fruits and vegetables. The compound is well known for its wide range of biological activities. In this study, 5-O-acyl derivatives of quercetin were synthesised and assessed for their antiproliferative activity against the HCT116 colon cancer and MDA-MB-231 breast cancer cell lines; and their radical scavenging activity against the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical species. Four derivatives were found to have improved the antiproliferative activity compared to quercetin whilst retaining radical scavenging activity.


Asunto(s)
Proliferación Celular/efectos de los fármacos , Depuradores de Radicales Libres/síntesis química , Depuradores de Radicales Libres/farmacología , Quercetina/síntesis química , Quercetina/farmacología , Benzotiazoles/química , Compuestos de Bifenilo/química , Línea Celular Tumoral , Flavonoides/síntesis química , Flavonoides/farmacología , Células HCT116 , Humanos , Picratos/química , Ácidos Sulfónicos/química
3.
Molecules ; 26(6)2021 Mar 18.
Artículo en Inglés | MEDLINE | ID: mdl-33803643

RESUMEN

Unprotected exposure of skin to solar ultraviolet radiation (UVR) may damage the DNA of skin cells and can lead to skin cancer. Sunscreens are topical formulations used to protect skin against UVR. The active ingredients of sunscreens are UV filters that absorb, scatter, and/or reflect UVR. Preventing the formation of free radicals and repairing DNA damages, natural antioxidants are also added to sunscreens as a second fold of protection against UVR. Antioxidants can help stabilise these formulations during the manufacturing process and upon application on skin. However, UV filters and antioxidants are both susceptible to degradation upon exposure to sunlight and oxygen. Additionally, due to their poor water solubility, natural antioxidants are challenging to formulate and exhibit limited penetration and bioavailability in the site of action (i.e., deeper skin layers). Cyclodextrins (CDs) are cyclic oligosaccharides that are capable of forming inclusion complexes with poorly soluble drugs, such as antioxidants. In this review, we discuss the use of CDs inclusion complexes to enhance the aqueous solubility of antioxidants and chemical UV filters and provide a protective shield against degradative factors. The role of CDs in providing a controlled drug release profile from sunscreens is also discussed. Finally, incorporating CDs inclusion complexes into sunscreens has the potential to increase their efficiency and hence improve their skin cancer prevention.


Asunto(s)
Ciclodextrinas/farmacología , Neoplasias Cutáneas/prevención & control , Protectores Solares/farmacología , Antioxidantes/administración & dosificación , Antioxidantes/química , Antioxidantes/farmacología , Ciclodextrinas/administración & dosificación , Ciclodextrinas/química , Daño del ADN , Preparaciones de Acción Retardada , Composición de Medicamentos , Estabilidad de Medicamentos , Depuradores de Radicales Libres/administración & dosificación , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Humanos , Estructura Molecular , Piel/efectos de los fármacos , Piel/metabolismo , Piel/efectos de la radiación , Solubilidad , Protectores Solares/administración & dosificación , Protectores Solares/química , Rayos Ultravioleta/efectos adversos
4.
Molecules ; 26(4)2021 Feb 13.
Artículo en Inglés | MEDLINE | ID: mdl-33668450

RESUMEN

Greek oregano and common oregano were compared in respect of the antioxidant and antibacterial activity of the corresponding essential oils and hydroethanolic extracts in relation with their chemical profile. The chemical composition of essential oils was determined by GC-MS and GC-FID, while extracts (phenolic acids and flavonoids fractions) were analyzed by HPLC-DAD. Based on given volatiles, the investigated subspecies represented two chemotypes: a carvacrol/γ-terpinene/p-cymene type in the case of Greek oregano and a sabinyl/cymyl type rich in terpinen-4-ol in common oregano. Within non-volatile phenolic compounds, rosmarinic acid appeared to dominate in both subspecies. Lithospermic acid B, chlorogenic acid and isovitexin were present only in Greek oregano extracts. However, the total content of flavonoids was higher in common oregano extracts. The essential oil and extract of Greek oregano revealed visibly stronger antibacterial activity (expressed as MIC and MBC) than common oregano, whereas the antioxidant potential (determined by DPPH, ABTS and FRAP) of these extracts was almost equal for both subspecies. In the case of Origanum plants, the potential application of essential oils and extracts as antiseptic and antioxidant agents in the food industry should be preceded by subspecies identification followed by recognition of their chemotype concerning both terpene and phenolics composition.


Asunto(s)
Antibacterianos/farmacología , Antioxidantes/farmacología , Etanol/química , Aceites Volátiles/farmacología , Origanum/química , Extractos Vegetales/farmacología , Agua/química , Benzotiazoles/química , Compuestos de Bifenilo/química , Cromatografía Líquida de Alta Presión , Flavonoides/análisis , Depuradores de Radicales Libres/farmacología , Pruebas de Sensibilidad Microbiana , Fenoles/análisis , Picratos/química , Reproducibilidad de los Resultados , Ácidos Sulfónicos/química
5.
Molecules ; 26(4)2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-33672029

RESUMEN

Exposure to reactive oxygen species can easily result in serious diseases, such as hyperproliferative skin disorders or skin cancer. Herbal extracts are widely used as antioxidant sources in different compositions. The importance of antioxidant therapy in inflammatory conditions has increased. Innovative formulations can be used to improve the effects of these phytopharmacons. The bioactive compounds of Plantago lanceolata (PL) possess different effects, such as anti-inflammatory, antioxidant, and bactericidal pharmacological effects. The objective of this study was to formulate novel liquid crystal (LC) compositions to protect Plantago lanceolata extract from hydrolysis and to improve its effect. Since safety is an important aspect of pharmaceutical formulations, the biological properties of applied excipients and blends were evaluated using assorted in vitro methods on HaCaT cells. According to the antecedent toxicity screening evaluation, three surfactants were selected (Gelucire 44/14, Labrasol, and Lauroglycol 90) for the formulation. The dissolution rate of PL from the PL-LC systems was evaluated using a Franz diffusion chamber apparatus. The antioxidant properties of the PL-LC systems were evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and malondialdehyde (MDA) assessments. Our results suggest that these compositions use a nontraditional, rapid-permeation pathway for the delivery of drugs, as the applied penetration enhancers reversibly alter the barrier properties of the outer stratum corneum. These excipients can be safe and highly tolerable thus, they could improve the patient's experience and promote adherence.


Asunto(s)
Composición de Medicamentos , Cristales Líquidos/química , Extractos Vegetales/farmacología , Plantago/química , Piel/efectos de los fármacos , Compuestos de Bifenilo/química , Proliferación Celular/efectos de los fármacos , Proliferación Celular/efectos de la radiación , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Impedancia Eléctrica , Depuradores de Radicales Libres/farmacología , Humanos , Peroxidación de Lípido/efectos de los fármacos , Peroxidación de Lípido/efectos de la radiación , Malondialdehído/metabolismo , Permeabilidad , Picratos/química , Piel/efectos de la radiación , Rayos Ultravioleta
6.
Molecules ; 26(4)2021 Feb 18.
Artículo en Inglés | MEDLINE | ID: mdl-33670764

RESUMEN

Phytochemical investigation of leaves and stembark of Artocarpus lacucha collected in Thailand resulted in three yet undescribed isomeric flavan-3-ol derivatives (1-3), the four known compounds gambircatechol (4), (+)-catechin (5), (+)-afzelechin (6) and the stilbene oxyresveratrol (7). Compounds 1 to 3 feature 6/6/5/6/5/6 core structures. All structures were deduced by NMR and MS, while density functional theory (DFT) calculations on B3LYP theory level were performed of compounds 1 to 3 to support the stereochemistry in positions 2 and 3 in the C-ring. Possible biosynthetic pathways leading to 4 are discussed. The DPPH assay revealed high radical scavenging activities for 1 (EC50 = 9.4 ± 1.0 µmol mL-1), 2 (12.2 ± 1.1), 3 (10.0 ± 1.5) and 4 (19.0 ± 2.6), remarkably lower than ascorbic acid (EC50 = 34.9) and α-tocopherol (EC50 = 48.6). A cytotoxicity assay revealed moderate but consistent antiproliferative properties of 1 in CH1/PA-1 (ovarian teratocarcinoma) and SW480 (colon carcinoma) cells, with IC50 values of 25 ± 6 and 34 ± 4 µM, respectively, whereas effects in A549 (non-small cell lung cancer) cells were rather negligible. The performed DCFH-DA assay of 1 in the former cell lines confirmed potent antioxidative effects even in the cellular environment.


Asunto(s)
Artocarpus/química , Flavonoides/farmacología , Depuradores de Radicales Libres/farmacología , Antioxidantes/farmacología , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Simulación por Computador , Flavonoides/química , Humanos , Espectroscopía de Resonancia Magnética , Conformación Molecular , Oxidación-Reducción , Estereoisomerismo
7.
Molecules ; 26(4)2021 Feb 12.
Artículo en Inglés | MEDLINE | ID: mdl-33673167

RESUMEN

The leaves of Homalomena aromatica are traditionally used in Bangladesh for the treatment of different chronic ailments. The purpose of this study was to explore in vitro antioxidant, thrombolytic activities, and in vivo neuropharmacological effects of methanolic extract of Homalomena aromatica (MEHA) leaves. Antioxidant activity of MEHA was assessed by a DPPH free radical scavenging assay and total phenolics content, total flavonoids content were also measured. The thrombolytic activity was determined by percentage of clot lysis and neuropharmacological activities by hole board, tail suspension, forced swimming and elevated plus maze tests. The results showed that the IC50 value of the extract against DPPH was 199.51 µg/mL. Quantitative analysis displayed higher contents of phenolics and flavonoids (147.71 mg gallic acid equivalent/g & 66.65 mg quercetin equivalent/g dried extract, respectively). The extract also showed a significant clot lysis (33.31%) activity. In case of anxiolytic activity, the elevate plus maze (EPM) test demonstrated an increase in time spent in open arms, and in case of hole board test, the number of head dipping was also significantly increased (p < 0.05). All the test compared with control (1% Tween in water) and standard (diazepam 1 mg/kg), significant dose (200 & 400 mg/kg) dependent anxiolytic activity was found. In antidepressant activity, there was a significant decrease in period of immobility in both test models (tail suspension and forced swimming) (p < 0.05). Moreover, 13 compounds were identified as bioactive, showed good binding affinities to xanthine oxidoreductase, tissue plasminogen activator receptor, potassium channel receptor, human serotonin receptor targets in molecular docking experiments. Furthermore, ADME/T analysis revealed their drug-likeness, likely pharmacological actions and non-toxic upon consumption. Taken together, our finding support the traditional medicinal use of this plant, which may provide a potential source for future drug discovery.


Asunto(s)
Antioxidantes/química , Araceae/química , Fibrinolíticos/química , Extractos Vegetales/química , Animales , Antidepresivos/química , Antidepresivos/farmacología , Antioxidantes/farmacología , Compuestos de Bifenilo/química , Simulación por Computador , Tiempo de Lisis del Coágulo de Fibrina , Fibrinolíticos/farmacología , Flavonoides/química , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Humanos , Ratones , Simulación del Acoplamiento Molecular , Neurofarmacología , Fenoles/química , Picratos/química , Extractos Vegetales/farmacología , Hojas de la Planta/química , Receptores de Serotonina/química , Antagonistas de la Serotonina/química , Antagonistas de la Serotonina/farmacología , Natación
8.
Medicine (Baltimore) ; 100(8): e24811, 2021 Feb 26.
Artículo en Inglés | MEDLINE | ID: mdl-33663100

RESUMEN

BACKGROUND: We performed a randomized clinical trial protocol to assess the effectiveness of edaravone for acute stroke. We hypothesized that edaravone is beneficial in improving neurological impairment resulting from acute stroke. METHOD: The protocol was reviewed and approved by the Research Ethics Board of Affiliated Hospital of Chengde Medical University (0092-2394), each participant signed a written consent before participating, and SPIRIT guidelines were followed throughout. The inclusion criteria for patients were as follows: diagnosed as acute stroke (ischemic stroke or intracerebral hemorrhage) by head CT or MRI within 72 hours; age greater than 18; motor function disorder; Glasgow Coma Scale greater than 12. Patients with the following symptoms were excluded: concurrent serious complications, such as coma, drug allergy, mental disorder, and other severe organic lesions in the brain. Sixty patients were finally included in the study. The control group accepted conventional treatment, while the treatment group received edaravone treatment on top of the conventional treatment of the control group. After treatment, the differences in functional movement, living ability score, neurological score, treatment effect, and adverse reaction of these 2 groups were tested and compared. DISCUSSION: As aging worsens, the incidence of acute stroke continues to increase. Brain damage will induce the production of oxygen radicals, which can damage the cytomembrane of brain cells and finally damage the nervous system and cause cerebral injury as well as the cerebral edema. Edaravone is an antioxidant and oxygen radical scavenger that can inhibit lipid peroxidation during the scavenging of oxygen free radicals. Besides, it can also elicit anti-inflammatory protective effects for nerve cells, increase cerebral blood flow volume, prevent the aggravation of cerebral hypoperfusion toward necrosis, reduce nerve damage, and improve neurological functions and prognosis. This is the first randomized controlled trial to assess the efficacy of edaravone for treating acute stroke. High quality, large sample size, multicenter randomized trials are still required. TRIAL REGISTRATION: researchregistry6492.


Asunto(s)
Edaravona/uso terapéutico , Depuradores de Radicales Libres/uso terapéutico , Fármacos Neuroprotectores/uso terapéutico , Accidente Cerebrovascular/tratamiento farmacológico , Actividades Cotidianas , Circulación Cerebrovascular/efectos de los fármacos , Edaravona/farmacología , Femenino , Depuradores de Radicales Libres/farmacología , Humanos , Peroxidación de Lípido/efectos de los fármacos , Masculino , Fármacos Neuroprotectores/farmacología , Ensayos Clínicos Controlados Aleatorios como Asunto
9.
Molecules ; 26(4)2021 Feb 08.
Artículo en Inglés | MEDLINE | ID: mdl-33567661

RESUMEN

The study was undertaken to investigate the antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from Ipomoea obscura (L.) Ker Gawl. aqueous leaf extract. The UV-visible spectral analysis of the ZnO-NPs showed an absorption peak at 304 nm with a bandgap energy of 3.54 eV, which are characteristics of zinc nanoparticles. Moreover, the particles were of nano-size (~24.26 nm) with 88.11% purity and were agglomerated as observed through Scanning Electron Microscopy (SEM). The phyto-fabricated ZnO-NPs offered radical scavenging activity (RSA) in a dose-dependent manner with an IC50 of 0.45 mg mL-1. In addition, the genotoxicity studies of ZnO-NPs carried out on onion root tips revealed that the particles were able to significantly inhibit the cell division at the mitotic stage with a mitotic index of 39.49%. Further, the cytotoxic studies on HT-29 cells showed that the phyto-fabricated ZnO-NPs could arrest the cell division as early as in the G0/G1 phase (with 92.14%) with 73.14% cells showing early apoptotic symptoms after 24 h of incubation. The results of the study affirm the ability of phyto-fabricated ZnO-NPs from aqueous leaf extract of I. obscura is beneficial in the cytotoxic application.


Asunto(s)
Ipomoea/metabolismo , Nanopartículas/química , Óxido de Zinc/química , Óxido de Zinc/farmacología , Antineoplásicos/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacología , Antineoplásicos/toxicidad , Compuestos de Bifenilo/química , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/metabolismo , Depuradores de Radicales Libres/farmacología , Depuradores de Radicales Libres/toxicidad , Tecnología Química Verde , Células HT29 , Humanos , Pruebas de Mutagenicidad , Cebollas/efectos de los fármacos , Cebollas/genética , Picratos/química , Óxido de Zinc/metabolismo , Óxido de Zinc/toxicidad
10.
Int J Nanomedicine ; 16: 15-29, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33447027

RESUMEN

Purpose: The present study investigates the phytosynthesis of silver nanoparticles (AgNPs) using Perilla frutescens leaf extract, which acts as a reducing agent for the conversion of silver ions (Ag+) into AgNPs. P. frutescens leaf synthesized AgNPs (PF@AgNPs) were evaluated for biomedical properties including antibacterial, antioxidant and anticancer activities. Materials and Methods: PF@AgNPs were synthesized using P. frutescens leaf extract and silver nitrate solution. The morphology and physical properties of PF@AgNPs were studied by spectroscopic techniques including, UV-Vis, FTIR, TEM, XRD, DLS, and TGA. Antibacterial activity of PF@AgNPs was evaluated by disk diffusion assay. Antioxidant activity of PF@AgNPs was checked by 2.2-diphenyl-1-picrylhydrazyl (DPPH), and 2.2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assays. Anticancer activity of PF@AgNPs was checked by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Cytotoxic effects of PF@AgNPs on most susceptible cancer cell lines were observed by phase contrast microscopy. Results: PF@AgNPs showed surface plasmon resonance peak at 461 nm. XRD pattern showed that the PF@AgNPs were face-centered cubic crystals with a mean size of 25.71 nm. TEM analysis revealed the different shapes (spherical, rhombic, triangle, and rod) of PF@AgNPs. Zeta potential value (-25.83 mV) indicated that PF@AgNPs were long-term stable and not agglomerated. A low polydispersity index value (0.389) indicated the monodispersity of PF@AgNPs. TGA revealed the high thermal stability of PF@AgNPs. PF@AgNPs exhibited maximum inhibition against Escherichia coli, followed by Bacillus subtilis and Staphylococcus aureus. PF@AgNPs showed maximum inhibition of 68.02 and 62.93% against DPPH and ABTS-free radicals, respectively. PF@AgNPs showed significant anticancer activity against human colon cancer (COLO205) and prostate adenocarcinoma (LNCaP). PF@AgNPs exhibited apoptotic effects on LNCaP cells including cell shrinkage, membrane blebbing, chromatin condensation, fragmentation of nuclei, and formation of apoptotic bodies. Conclusion: The present study reports the successful synthesis of PF@AgNPs using P. frutescens leaf extract. The synthesized PF@AgNPs are FCC crystals, monodispersed, long-term stable, and non-agglomerated. The observed antibacterial, antioxidant, and anticancer activities demonstrate the potential biomedical applications of PF@AgNPs.


Asunto(s)
Antibacterianos/farmacología , Antineoplásicos/farmacología , Antioxidantes/farmacología , Nanopartículas del Metal/química , Perilla frutescens/química , Extractos Vegetales/química , Hojas de la Planta/química , Plata/farmacología , Bacillus subtilis/efectos de los fármacos , Compuestos de Bifenilo/química , Línea Celular Tumoral , Color , Dispersión Dinámica de Luz , Escherichia coli/efectos de los fármacos , Depuradores de Radicales Libres/farmacología , Humanos , Nanopartículas del Metal/ultraestructura , Pruebas de Sensibilidad Microbiana , Picratos/química , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Staphylococcus aureus/efectos de los fármacos , Electricidad Estática , Termogravimetría , Difracción de Rayos X
11.
Mar Drugs ; 19(2)2021 Jan 27.
Artículo en Inglés | MEDLINE | ID: mdl-33513822

RESUMEN

Inorganic polyphosphate (polyP) is a widely distributed polymer found from bacteria to animals, including marine species. This polymer exhibits morphogenetic as well as antiviral activity and releases metabolic energy after enzymatic hydrolysis also in human cells. In the pathogenesis of the coronavirus disease 2019 (COVID-19), the platelets are at the frontline of this syndrome. Platelets release a set of molecules, among them polyP. In addition, the production of airway mucus, the first line of body defense, is impaired in those patients. Therefore, in this study, amorphous nanoparticles of the magnesium salt of polyP (Mg-polyP-NP), matching the size of the coronavirus SARS-CoV-2, were prepared and loaded with the secondary plant metabolite quercetin or with dexamethasone to study their effects on the respiratory epithelium using human alveolar basal epithelial A549 cells as a model. The results revealed that both compounds embedded into the polyP nanoparticles significantly increased the steady-state-expression of the MUC5AC gene. This mucin species is the major mucus glycoprotein present in the secreted gel-forming mucus. The level of gene expression caused by quercetin or with dexamethasone, if caged into polyP NP, is significantly higher compared to the individual drugs alone. Both quercetin and dexamethasone did not impair the growth-supporting effect of polyP on A549 cells even at concentrations of quercetin which are cytotoxic for the cells. A possible mechanism of the effects of the two drugs together with polyP on mucin expression is proposed based on the scavenging of free oxygen species and the generation of ADP/ATP from the polyP, which is needed for the organization of the protective mucin-based mucus layer.


Asunto(s)
Antiinflamatorios/farmacología , Antioxidantes/farmacología , Dexametasona/farmacología , Mucina 5AC/biosíntesis , Mucina 5AC/efectos de los fármacos , Quercetina/farmacología , Células A549 , Antiinflamatorios/química , Antioxidantes/química , Dexametasona/química , Depuradores de Radicales Libres/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Magnesio/química , Mucina 5AC/genética , Mucinas/biosíntesis , Mucinas/química , Nanopartículas , Tamaño de la Partícula , Plantas/química , Polifosfatos/química , Quercetina/química , Especies Reactivas de Oxígeno
12.
Molecules ; 26(2)2021 Jan 14.
Artículo en Inglés | MEDLINE | ID: mdl-33466694

RESUMEN

This study was focused on extraction, radical scavenging activities, and chemical composition identification of total flavonoids in sunflower (Helianthus annuus L.) receptacles (TFSR). We investigated the optimal extract parameters of TFSR using response surface methodology. The highest yield of TFSR was 1.04% with the ethanol concentration 58%, the material-to-liquid ratio 1:20 (v/w), the extraction time 2.6 h, and the extraction temperature 67 °C. The results of radical scavenging activities showed that ethyl acetate fraction (EAF) was the strongest by using 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azino-bis (3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS) and iron ion reducing analysis. The EAF had the highest flavonoids contents. Four fractions A, B, C and D were enrichment from EAF by polyamide resin. Fraction B had the highest flavonoids content. Thirteen chemical components of flavonoids in fraction B were first identified by Ultimate 3000 Nano LC System coupled to a Q Exactive HF benchtop Orbitrap mass spectrometer (UHPLC-HRMS/MS). Among of the thirteen chemical components, isoquercetin and daidzein were identified accurately by comparing with standard samples. Radical scavenging analysis showed that isoquercetin and EAF had strong activities. Therefore, sunflower receptacles can be used as a source of natural flavonoids. TFSR as a natural radical scavenger has potential applications in pharmaceutical industry.


Asunto(s)
Acetatos/química , Antioxidantes/farmacología , Flavonoides/química , Flavonoides/farmacología , Depuradores de Radicales Libres/farmacología , Helianthus/química , Extractos Vegetales/farmacología , Flavonoides/aislamiento & purificación
13.
Int J Biol Macromol ; 171: 320-330, 2021 Feb 28.
Artículo en Inglés | MEDLINE | ID: mdl-33421468

RESUMEN

The water-soluble non-starch polysaccharides isolated from natural resources have become research hotpots in the field of food science and human health due to widely distributed in nature and low toxicity. It has indicated that the health-promoting effect of water-soluble non-starch polysaccharides were partly attributable to against excessive oxidative stress. Indeed, excessive oxidative stress in the body has been reported in occurrence of disease. The water-soluble non-starch polysaccharides from natural resources exhibit antioxidant activity to against oxidative stress via scavenging free radicals promoting antioxidant enzymes activity and/or regulating antioxidant signaling pathways. In this review, the water-soluble non-starch polysaccharides as medicine agent and the factor affecting antioxidant as well as the relationship between oxidative stress and disease are summarized, and the mechanisms of water-soluble non-starch polysaccharides therapy in disease are also discussed. It will provide a theoretical basis for natural polysaccharides used for the treatment of diseases.


Asunto(s)
Antioxidantes/farmacología , Productos Biológicos/farmacología , Estrés Oxidativo/efectos de los fármacos , Polisacáridos/farmacología , Antioxidantes/aislamiento & purificación , Antioxidantes/uso terapéutico , Productos Biológicos/aislamiento & purificación , Productos Biológicos/uso terapéutico , Enfermedades Cardiovasculares/tratamiento farmacológico , Fraccionamiento Químico/métodos , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Humanos , Enfermedades Renales/tratamiento farmacológico , Hepatopatías/tratamiento farmacológico , Enfermedades Pulmonares/tratamiento farmacológico , Estructura Molecular , Enfermedades Neurodegenerativas/tratamiento farmacológico , Orgánulos/efectos de los fármacos , Polisacáridos/aislamiento & purificación , Polisacáridos/uso terapéutico , Transducción de Señal/efectos de los fármacos , Enfermedades de la Piel/tratamiento farmacológico , Solubilidad , Agua
14.
Nat Commun ; 12(1): 640, 2021 01 28.
Artículo en Inglés | MEDLINE | ID: mdl-33510167

RESUMEN

Neural stem/progenitor cells (NSPCs) persist over the lifespan while encountering constant challenges from age or injury related brain environmental changes like elevated oxidative stress. But how oxidative stress regulates NSPC and its neurogenic differentiation is less clear. Here we report that acutely elevated cellular oxidative stress in NSPCs modulates neurogenic differentiation through induction of Forkhead box protein O3 (FOXO3)-mediated cGAS/STING and type I interferon (IFN-I) responses. We show that oxidative stress activates FOXO3 and its transcriptional target glycine-N-methyltransferase (GNMT) whose upregulation triggers depletion of s-adenosylmethionine (SAM), a key co-substrate involved in methyl group transfer reactions. Mechanistically, we demonstrate that reduced intracellular SAM availability disrupts carboxymethylation and maturation of nuclear lamin, which induce cytosolic release of chromatin fragments and subsequent activation of the cGAS/STING-IFN-I cascade to suppress neurogenic differentiation. Together, our findings suggest the FOXO3-GNMT/SAM-lamin-cGAS/STING-IFN-I signaling cascade as a critical stress response program that regulates long-term regenerative potential.


Asunto(s)
Proteína Forkhead Box O3/metabolismo , Interferón Tipo I/metabolismo , Laminas/metabolismo , Estrés Oxidativo , Procesamiento Proteico-Postraduccional , Acetilcisteína/farmacología , Animales , Diferenciación Celular/efectos de los fármacos , Células Cultivadas , Depuradores de Radicales Libres/farmacología , Glicina N-Metiltransferasa/metabolismo , Células HEK293 , Herbicidas/farmacología , Humanos , Ratones , Células-Madre Neurales/citología , Células-Madre Neurales/efectos de los fármacos , Células-Madre Neurales/metabolismo , Paraquat/farmacología , S-Adenosilmetionina/metabolismo , Transducción de Señal
15.
Acta Pharm ; 71(1): 131-141, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-32697745

RESUMEN

This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTS∙+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 µmol L-1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy.


Asunto(s)
Antioxidantes/farmacología , Daño del ADN , Peróxido de Hidrógeno/antagonistas & inhibidores , Peróxido de Hidrógeno/toxicidad , Iridoides/farmacología , Linfocitos/efectos de los fármacos , Aceite de Oliva/química , Alcohol Feniletílico/análogos & derivados , Antioxidantes/química , Relación Dosis-Respuesta a Droga , Depuradores de Radicales Libres/farmacología , Humanos , Iridoides/química , Alcohol Feniletílico/química , Alcohol Feniletílico/farmacología
16.
Carbohydr Polym ; 251: 117078, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-33142621

RESUMEN

The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose. The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-ß-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.


Asunto(s)
Antioxidantes/farmacología , Depuradores de Radicales Libres/farmacología , Radical Hidroxilo/química , Monosacáridos/química , Pinus/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Antioxidantes/química , Arabinosa/química , Depuradores de Radicales Libres/química , Alimentos Funcionales , Galactosa/química , Glucosa/química , Manosa/química , Oxidación-Reducción , Polisacáridos/química , Ramnosa/química
17.
Carbohydr Polym ; 251: 117125, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-33142657

RESUMEN

Polysaccharide-based nanofibers from Tragacanth Gum (TG) and polyethylene terephthalate (PET) were post-treated with selenium nanoparticles (Se NPs) and also stabilized with TG (SeNPs/TG). DLS, FE-SEM, EDX, TEM, and XRD were employed to verify the synthesis of Se NPs. The relatively narrow size distribution of SeNPs/TG showed through TEM and DLS investigations comparing with Se NPs. The Se NPs formation with and without TG was studied with FTIR confirmed the final stabilized solution due to the bonded hydroxyl groups of TG with Se NPs. Also, a relatively higher antioxidant reported on SeNPs/TG at 0.5-5 mg/mL using DPPH scavenging ability. The Se NPs and SeNPs/TG solutions specified remarkable inhibition against Staphylococcus aureus and Candida albicans; however, no significant antibacterial activities observed on the treated nanofibers. Finally, the uniform migration of fibroblast cells in wound healing of the treated nanofibers with SeNPs/TG proved the value of the products in medical applications.


Asunto(s)
Antiinfecciosos/farmacología , Depuradores de Radicales Libres/farmacología , Nanocompuestos/administración & dosificación , Nanofibras/química , Tereftalatos Polietilenos/química , Selenio/química , Tragacanto/química , Antiinfecciosos/química , Candida albicans/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Depuradores de Radicales Libres/química , Humanos , Nanocompuestos/química , Polisacáridos/química , Staphylococcus aureus/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos
18.
Carbohydr Polym ; 254: 117199, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-33357840

RESUMEN

Hydroxybutyl chitosan (HBC) with different degree of substitution (DS) were prepared using a homogeneous reaction system (KOH/urea), which could achieve temperature-dependent reversible morphological transition in aqueous solution. During hydrophobic assembly, amino groups on HBC chains exposed on the surface of nanoparticles formed a poly-cationic structure. The structure of HBCs was characterized by FTIR, 13C NMR, XRD, TGA and rheology. The morphology and assembly mechanism of HBC nanoparticles were studied by TEM, AFM and DLS. Also, the results of coagulation, bacteriostatic, superoxide anion clearance and anionic contaminant removal tests suggested that HBC nanoparticles had excellent flocculation and removal effect of anionic composites. Moreover, the cytocompatibility test indicated that HBC could effectively promote proliferation and division of mouse fibroblast, mouse embryonic fibroblast and rat bone marrow mesenchymal stem cells. These cationic HBC nanoparticles exhibited great potential in multi-functional applications.


Asunto(s)
Quitosano/análogos & derivados , Nanopartículas/química , Animales , Antibacterianos/química , Antibacterianos/farmacología , Cationes/química , Línea Celular , Proliferación Celular/efectos de los fármacos , Quitosano/química , Quitosano/farmacología , Quitosano/toxicidad , Escherichia coli/efectos de los fármacos , Floculación/efectos de los fármacos , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Tecnología Química Verde , Hemólisis/efectos de los fármacos , Interacciones Hidrofóbicas e Hidrofílicas , Técnicas In Vitro , Ensayo de Materiales , Células Madre Mesenquimatosas/efectos de los fármacos , Ratones , Estructura Molecular , Células 3T3 NIH , Nanopartículas/toxicidad , Conejos , Ratas , Staphylococcus aureus/efectos de los fármacos , Temperatura , Temperatura de Transición
19.
Food Chem ; 340: 128123, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33010645

RESUMEN

Six commercial red sorghum varieties (Tong Za 117, 141, 142 and 143, Chi Za 109 and 101) were investigated for their triacylglycerol (TAG) profiles, soluble and bound phenolics, and radical scavenging and anti-inflammatory activities. A total of 21 TAGs were identified in red sorghum oils for the first time. Total phenolic (TPC) and flavonoid contents (TFC) in the soluble or bound phenolic fractions differed among red sorghums. Significant correlation among TPC, TFC and DPPH radical scavenging activities was observed in both fractions. Except for caffeic acid, most of phenolic acids in red sorghums are in the bound form. Soluble 3-deoxyanthocyanidins contents (2.12-57.14 µg/g) were significantly higher than those of bound forms (0.01-0.18 µg/g) regardless of sorghum varieties and types of 3-deoxyanthocyanidins. Moreover, the stronger anti-inflammatory capacity of soluble phenolic fraction in Tong Za 117 correlated with its higher TPC, TFC and radical scavenging activity than those of its bound counterpart.


Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Depuradores de Radicales Libres/farmacología , Sorghum/química , Triglicéridos/análisis , Triglicéridos/farmacología , Animales , Antiinflamatorios no Esteroideos/química , Antioxidantes/química , Ácidos Cafeicos/análisis , Ácidos Cafeicos/química , Diterpenos/análisis , Flavonoides/análisis , Depuradores de Radicales Libres/química , Hidrólisis , Hidroxibenzoatos/análisis , Hidroxibenzoatos/química , Ratones , Fenoles , Extractos Vegetales/química , Aceites Vegetales/análisis , Aceites Vegetales/química , Células RAW 264.7
20.
J Ethnopharmacol ; 266: 113408, 2021 Feb 10.
Artículo en Inglés | MEDLINE | ID: mdl-32979409

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: The preparations of Phlomis aerial parts are used traditionally in Anatolia for wound healing and in inflammatory disorders. METHODS: For the identification of the active fraction, the air dried aerial parts of Phlomis rigida Labill. were extracted by methanol and fractionated successively by n-hexane, dichloromethane and ethyl acetate, respectively. The phenolic constituents were characterized by the Folin-Ciocaltheu method; the antioxidant activity was performed by ABTS and DPPH radical scavenging assays. In vitro anti-inflammatory activity was evaluated by LOX enzyme inhibition, spectrophotometrically as well as cell cultures. The wound healing properties of P. rigida extract gels were studied via in vitro cell culture methods and in vivo by excisional wound model using Balb-c mice. The P. rigida extract was analyzed and characterized by GC-FID, GC-MS, and LC-MS. RESULTS: The P. rigida methanol extract showed moderate LOX inhibitory at IC50 = 19.5 ± 2.8 µg/mL whereas the antioxidant activity was by DPPH• IC50 = 0.89 mg/mL, and by ABTS• IC50 = 0.99 mg/mL, respectively. In addition, a remarkable P. rigida extracts anti-inflammatory activity was observed in the cell culture assay, which was then confirmed by the in vitro wound healing activity applied at 0.125-0.5 mg/mL concentrations, resulting in a dose-dependent increase in wound closure at the final stage. The P. rigida gel formulation was prepared to evaluate the extract in vivo, whereas the experimental results of the new gel formulation supported the findings of the in vitro wound healing activity. CONCLUSION: The findings of this in vitro and in vivo study suggest that the wound healing and anti-inflammatory properties provide a scientific evidence of the ethnopharmacological application of Phlomis species.


Asunto(s)
Analgésicos/farmacología , Antiinflamatorios/farmacología , Phlomis/química , Extractos Vegetales/farmacología , Analgésicos/administración & dosificación , Analgésicos/aislamiento & purificación , Animales , Antiinflamatorios/administración & dosificación , Antiinflamatorios/aislamiento & purificación , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Depuradores de Radicales Libres/administración & dosificación , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Inflamación/tratamiento farmacológico , Inflamación/patología , Concentración 50 Inhibidora , Ratones , Ratones Endogámicos BALB C , Extractos Vegetales/administración & dosificación , Células RAW 264.7 , Cicatrización de Heridas/efectos de los fármacos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...