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1.
Medicine (Baltimore) ; 98(48): e18115, 2019 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-31770237

RESUMEN

INTRODUCTION: The aim of this report is to present a case of an apically involved tooth with successful regeneration by only applying enamel matrix derivative. The root of the tooth was planed and the defect area was well debrided using various instruments, including curettes and an ultrasonic scaler, and the root surface of the tooth and the defect area were loaded with enamel matrix derivative. PATIENT CONCERNS: A 32-year-old man visited the clinic due to a referral for the evaluation of his mandibular left first molar. DIAGNOSIS: The clinical and radiographic assessment displayed the loss of the periodontium around the tested tooth with apical involvement of the mesial root. Bleeding upon probing was noted at the mandibular first molar, with the deepest periodontal probing depth of 15 mm. INTERVENTIONS: A nonsurgical approach was firstly performed on the tooth, and the deepest probing depth was reduced to 12 mm. After re-evaluation, elevation of a full-thickness flap was done, the root of the tooth was planed, and the defect area was well debrided using various instruments, including curettes and an ultrasonic scaler. The defect area on the mandibular left first molar was grafted with enamel matrix derivative. OUTCOMES: The 7-month postoperative clinical and radiographic evaluation showed healthy gingiva and an increase in radiopacity. The final 1-year and 9-month postoperative evaluation showed that regeneration of bony defect was well maintained up to the final evaluation with reduction of probing depth. CONCLUSION: In conclusion, a case of apically involved tooth can be treated only with enamel matrix derivative after meticulous debridement with curettes and an ultrasonic scaler.


Asunto(s)
Productos Biológicos/uso terapéutico , Matriz Ósea/trasplante , Esmalte Dental/trasplante , Ápice del Diente/cirugía , Odontopatías/cirugía , Adulto , Raspado Dental/métodos , Humanos , Masculino , Mandíbula/cirugía , Diente Molar/patología , Diente Molar/cirugía , Periodoncio/patología , Periodoncio/cirugía , Ápice del Diente/patología , Odontopatías/patología
2.
Medicine (Baltimore) ; 98(40): e17356, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31577730

RESUMEN

Molar-incisor malformation (MIM) is a recently defined dental abnormality of molar root and incisors, and introduced as one of the causes of periapical and periodontal abscess. The purpose of this study was to investigate the clinical and radiological features of MIM, with special emphasis on various medical history. A total of 38 patients with MIM were included in this study. Radiographic features and clinical data, including medical history, chief complaint, associated complications, treatment, and prognosis, were retrospectively investigated. On radiographs, the affected molars showed short, slender, underdeveloped roots and constricted pulp chambers. All affected incisors and canines exhibited dilacerated short roots, wedge-shaped defect on the cervical part of the crown. Complications included periodontal bone loss (52.6%), endodontic lesion (50.0%), and endodontic-periodontal lesion (28.9%). The medical histories of the patients with MIM indicate that almost all (94.7%) were hospitalized due to problems during the neonatal period. MIM may cause various dental problems, such as periapical and periodontal abscess and early loss of the affected teeth. The early diagnosis of MIM on radiographs and appropriate treatment will contribute to a favorable prognosis, especially for young and adolescent patients.


Asunto(s)
Incisivo/diagnóstico por imagen , Incisivo/patología , Diente Molar/diagnóstico por imagen , Diente Molar/patología , Anomalías Dentarias/diagnóstico por imagen , Anomalías Dentarias/patología , Adolescente , Pérdida de Hueso Alveolar/etiología , Niño , Preescolar , Cavidad Pulpar/diagnóstico por imagen , Cavidad Pulpar/patología , Femenino , Humanos , Masculino , Radiografía Dental , Estudios Retrospectivos , Anomalías Dentarias/complicaciones , Anomalías Dentarias/diagnóstico , Raíz del Diente/diagnóstico por imagen , Raíz del Diente/patología , Adulto Joven
3.
J Ayub Med Coll Abbottabad ; 31(3): 418-421, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31535519

RESUMEN

BACKGROUND: This study was conducted to compare the adaptation of resin-based sealants with that of Resin modified glass ionome-based sealants in various tooth fissure morphologies. METHODS: It was an in vitro experimental study done at the Dow University and NED University, Karachi, Pakistan. Ten extracted human molars were randomly assigned to two groups, (n=5) each. Fissure sealant material (Resin based sealant or resin modified glass ionomer-based sealant) was applied on the occlusal surface of the tooth according to manufacturer's recommendations. Specimens were thermocycled and then sectioned into three longitudinal parts in the bucco- lingual direction. Specimens were examined using scanning electron microscope for the adaptation of the sealant in the occlusal fissure. Mann-Whitney -U test and Kruskall-Wallis test were applied to compare the adaptability scores of sealant materials in the tooth fissure. Level of significance was kept at 0.05. RESULTS: There were no significant differences in the adaptability scores among U-shaped (p-value=0.35), V-shaped (p-value=0.89), IK-shaped (p-value=0.52), I-shaped (p-value- =0.41) and Y-shaped (p-value=1.00) fissure patterns. Similarly, there were no significant differences observed between the resin-based sealant (p-value=0.95) versus RMGIC based sealant (p-value=0.63) for the adaptability scores in various tooth fissure morphologies. CONCLUSIONS: No significant difference was found between resin-based sealants and resin modified glass ionomerbased sealants for the adaptation in various tooth fissure patterns.


Asunto(s)
Fisuras Dentales/patología , Cementos de Ionómero Vitreo , Diente Molar , Selladores de Fosas y Fisuras , Cementos de Ionómero Vitreo/química , Humanos , Diente Molar/efectos de los fármacos , Diente Molar/patología , Pakistán
4.
Am J Orthod Dentofacial Orthop ; 156(3): 355-364, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31474265

RESUMEN

INTRODUCTION: The aim of this work was to analyze data of patients with failed or delayed eruption of first and second permanent molars, to assess the effectiveness of the treatment methods used. METHODS: Epidemiologic and clinical data of 125 patients (mean age 14.08 ± 4.04 years) with 197 affected molars (30 first and 167 second molars) were retrospectively analyzed. The treatment outcome was known in 161 molars after patient drop-out (20 patients with 36 molars). The cases were categorized into 8 groups according to the choice of treatment: orthodontic uprighting, surgical-orthodontic uprighting, surgical uprighting, surgical repositioning, surgical exposure, first or second molar extraction, third molar extraction, or removal of pathologic conditions. RESULTS: The overall treatment outcome was positive in 141 molars (87.6%). It was positive in all cases treated with orthodontic uprighting (7 molars), surgical exposure (10 molars), surgical uprighting (38 molars), and surgical repositioning (8 molars), but it was significantly lower for surgical-orthodontic uprighting (34/48 molars, 70.8%). The positive outcome was significantly lower for inclusion (52/68 molars, 76.5%) than for early-diagnosed condition (11/11 molars, 100%) and retention (78/82 molars, 95.1%), and for total bone crown coverage (21/28 molars, 75.0%) than for osteomucosal or mucosal crown coverage (120/133 molars, 90.2%). CONCLUSIONS: This study demonstrates that an early diagnosis results in a better outcome regardless of the treatment used, with the number of cases with a positive outcome being higher in younger patients.


Asunto(s)
Diente Molar/patología , Anomalías Dentarias , Erupción Dental , Técnicas de Movimiento Dental/métodos , Diente Impactado/terapia , Adolescente , Adulto , Niño , Preescolar , Femenino , Humanos , Italia , Modelos Logísticos , Masculino , Diente Molar/cirugía , Tercer Molar/cirugía , Estudios Retrospectivos , Diente Impactado/diagnóstico , Diente Impactado/cirugía , Resultado del Tratamiento , Adulto Joven
5.
Braz Oral Res ; 33: e048, 2019 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-31432924

RESUMEN

This cross-sectional study aimed to assess the prevalence of molar incisor hypomineralization (MIH) and its relationship with the number of primary teeth with developmental defects of enamel (DDE). A representative population-based sample of 731 schoolchildren was randomly selected from the public school system in Curitiba, Brazil. Schoolchildren aged 8 years with fully erupted permanent first molars and incisors were eligible for the study. MIH and DDE were classified by four calibrated examiners (kappa > 0.75) according to EAPD criteria and to the FDI-modified DDE index. Clinical data were collected in a school environment. Socioeconomic information was collected through a self-administered semistructured questionnaire applied to the children's caregivers. Statistical analyses were carried out using Poisson multiple regression with robust variance (α = 0.05). MIH prevalence was 12.1% (95%CI: 10-15), and opacities were the most prevalent defect. Socioeconomic factors were not associated with MIH. Children with demarcated opacity in primary teeth presented a higher prevalence of MIH than those without DDE in primary teeth. In the multiple analysis, the increase of one primary tooth affected by demarcated opacity increased the prevalence of MIH by 33% (PR = 1.33, 95%CI: 1.15-1.53, p < 0.001). Asian children had a higher prevalence of MIH (PR = 2.91, 95%CI: 1.08-8.09 p = 0.035) than did Caucasian children.Conclusion: Based on these findings, the prevalence of MIH in Curitiba was 12.1%. Demarcated opacity in primary teeth could be considered a predictor of MIH.


Asunto(s)
Hipoplasia del Esmalte Dental/epidemiología , Diente Primario , Brasil/epidemiología , Niño , Estudios Transversales , Hipoplasia del Esmalte Dental/patología , Femenino , Humanos , Incisivo/patología , Masculino , Diente Molar/patología , Prevalencia , Análisis de Regresión , Factores de Riesgo , Autoinforme , Índice de Severidad de la Enfermedad , Factores Socioeconómicos , Diente Primario/patología
6.
Am J Orthod Dentofacial Orthop ; 156(2): 248-256.e2, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31375235

RESUMEN

INTRODUCTION: The treatment options for adults with increased overbite are limited to dentoalveolar changes that camouflage the condition. Because of high relapse tendency, defining the problem area is important when creating a treatment plan. This study aimed to evaluate dentoskeletal morphology in skeletal Class I and II anomalies associated with Angle Class I, Class II Division 1 (Class II/1), and Class II Division 2 (Class II/2) malocclusions with increased overbite compared with normal occlusion. METHODS: Pretreatment cephalograms of 306 patients (131 men, 175 women; overall ages 18-45 years) were evaluated. Four groups were constructed. Three groups had increased overbite (>4.5 mm): group 1 (n = 96) skeletal Class I (ANB = 0.5°-4°), group 2 (n = 85) skeletal Class II (ANB >4.5°) with Class II/1; and group 3 (n = 79) skeletal Class II with Class II/2 malocclusion. Group 4 as a control (n = 46) skeletal Class I normal overbite. Dental and skeletal characteristics of the groups were compared by sex. For statistical evaluations, analysis of variance followed by Tukey post hoc, Mann-Whitney U, and Kruskall-Wallis tests were used. Additionally correlation coefficients between overbite and skeletal/dental parameters were calculated. RESULTS: Between sexes, with regard to skeletal parameters, the men had greater values in millimetric measurements, and the women had higher SN/GoGn values. Maxillary/mandibular molar heights and the mandibular incisor heights were higher in men. In group 1, decreased lower anterior facial height (LAFH), retrusive mandibular incisors, and increased interincisal degree were determined. The maxillary molars were intrusive, whereas the vertical position of the mandibular molars and incisors in both jaws were normal. In group 2, retrognathic mandible, increased LAFH and mandibular plane angle, extrusive maxillary/mandibular incisors, protrusive mandibular incisors, and decreased interincisal degree were found. In group 3, decreased LAFH, increased interincisal degree, and retrusive incisors in both jaws were determined. There were significant negative correlations between SN/GoGN, palatal plane, and overbite in group 2 and between ANS-SN and overbite in group 3, and positive correlation between interinsical angle and overbite in all increased overbite groups. CONCLUSIONS: Dental morphology seems to be the main factor of increased overbite. Differences between groups were related primarily to inclinations and vertical positions of the incisors, rather than molar positions.


Asunto(s)
Maloclusión de Angle Clase II/patología , Maloclusión de Angle Clase I/patología , Sobremordida/epidemiología , Sobremordida/patología , Adolescente , Adulto , Análisis de Varianza , Puntos Anatómicos de Referencia , Cefalometría , Femenino , Humanos , Incisivo/patología , Masculino , Maloclusión de Angle Clase III/patología , Mandíbula/patología , Maxilar/patología , Persona de Mediana Edad , Diente Molar/patología , Retrognatismo/patología , Factores Sexuales , Turquia , Adulto Joven
7.
BMJ Case Rep ; 12(7)2019 Jul 23.
Artículo en Inglés | MEDLINE | ID: mdl-31340944

RESUMEN

A 6-year-old child with an episodic history of ulcerations over buccal mucosa was found to have severe inflammation on the palatal aspect of permanent first molars with grade 2 mobility bilaterally. Radiographical features were suggestive of bone loss around permanent molars extending to the distal aspect of the deciduous first molars. The clinical and radiographical findings were indicative of periodontal degeneration without any apparent cause visible intraorally. Further biopsy was done from the rashes present on the malar prominences, which showed nodular aggregates of atypical cells in superficial dermis. These large histiocytic cells with vesicular nuclei and nuclear grooves were immunopositive for CD1a and S100, concluding the diagnosis of Langerhans cell histiocytosis. For treatment, patient was referred to Department of Haemato-oncology and chemotherapy was suggested as per protocol.


Asunto(s)
Histiocitosis de Células de Langerhans/patología , Histiocitosis de Células de Langerhans/terapia , Úlceras Bucales/patología , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Biopsia con Aguja , Niño , Terapia Combinada , Diagnóstico Diferencial , Estudios de Seguimiento , Histiocitosis de Células de Langerhans/diagnóstico , Humanos , Inmunohistoquímica , Masculino , Diente Molar/patología , Mucosa Bucal/patología , Úlceras Bucales/diagnóstico , Radioterapia Ayuvante , Medición de Riesgo , Odontalgia/diagnóstico , Odontalgia/patología , Resultado del Tratamiento
8.
Biomed Res Int ; 2019: 4934128, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31317031

RESUMEN

Introduction: With an increasing demand for orthodontic treatment for adult patients, orthodontic professionals are constantly seeking novel strategies and technologies that can accelerate tooth movement in order to shorten the treatment period. For instance, in recent years, the influences of different surgical techniques on orthodontic tooth movement in the ipsilateral side of surgery were intensively investigated. Here, we attempt to examine if corticotomy could also affect the rate of tooth movement in the contralateral side of the surgery by using a rodent model. Materials and Methods: 72 eight-week-old Sprague-Dawley rats were randomly divided into three groups as follows: the Control group (orthodontic treatment devices delivered only, no tooth movement), the orthodontic tooth movement (OTM) group (orthodontic treatment devices delivered and orthodontic treatment performed), and the Corticotomy + OTM group (remote corticotomy performed, orthodontic treatment devices delivered, followed by orthodontic treatment). The surgical procedure was conducted on the right side of the maxilla at the time of appliance placement and a force of 60 g was applied between the maxillary left first molar and maxillary incisors using nickel-titanium springs to stimulate OTM. The OTM distance and speed were tracked at 3, 7, 14, and 28 days post-surgery, followed by histological and immunohistochemical assessments. Results: In comparison with orthodontic treatment only, the contralateral corticotomy significantly accelerated OTM. Furthermore, animals undergoing corticotomy + OTM presented with a greater number of osteoclasts on the compression side, stronger staining of the osteogenic marker on the tension side, and higher expression of an inflammatory marker than the OTM group animals. Conclusion: Our current study demonstrates that remote corticotomy effectively accelerates alveolar bone remodeling and OTM. The study enriches our understanding of the regional acceleratory phenomenon (RAP) and offers an alternative strategy for accelerating OTM to shorten the orthodontic treatment period.


Asunto(s)
Remodelación Ósea/efectos de los fármacos , Resorción Ósea/cirugía , Incisivo/cirugía , Osteogénesis/efectos de los fármacos , Animales , Modelos Animales de Enfermedad , Humanos , Incisivo/crecimiento & desarrollo , Incisivo/patología , Maxilar/crecimiento & desarrollo , Maxilar/patología , Maxilar/cirugía , Diente Molar/crecimiento & desarrollo , Diente Molar/patología , Diente Molar/cirugía , Níquel/uso terapéutico , Alambres para Ortodoncia , Osteoclastos/metabolismo , Osteoclastos/patología , Ratas , Titanio/uso terapéutico , Técnicas de Movimiento Dental
9.
Acta Odontol Latinoam ; 32(1): 44-48, 2019 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-31206574

RESUMEN

Clinically, Molar-Incisor Hypomineralization (MIH) lesions are not distributed symmetrically, and their severity varies even within the same arcade. AIM: To estimate the frequency of asymmetries in hypomineralized lesions on permanent molars and incisors of children with MIH. METHODS: Three pediatric dentists, calibrated following the diagnostic criteria of Mathu-Muju and Wright (2006) (Kappa 0.87) identified presence and severity of opacities on molars and incisors of patients with MIH. Six pairs of teeth (permanent maxillary and mandibular first molars, central and lateral incisors) were evaluated in each patient. Degree of lesion severity (0-none, 1-mild, 2-moderate, 3-severe) was recorded for each tooth. For each pair containing any affected teeth, asymmetry of presence (one tooth in the pair with lesion and the other intact), asymmetry of severity (both teeth with lesions but with different degrees of severity) or symmetry of severity (both affected teeth with the same degree of severity) were evaluated. The recorded values were entered into a database to calculate percentages, 95% confidence intervals and Chi-Square test for comparisons. RESULTS: The sample consisted of475 of the 1032 pairs of teeth evaluated in the 172 patients included in the study, mean age 11±2.2 years, and 50% female. Asymmetry was found for 67.5% (63.1 - 71.7) of the pairs of the studied teeth. There was a significant relationship between asymmetries and symmetries (p=0.038). A total 50.1% of the pairs were asymmetrical for presence of opacities. Of these, 62.2% scored severity 1 (mild). Symmetry of severity was found for 32.5% of the lesions. Among the pairs of affected teeth, the most frequently observed degrees of lesion severity were mild and moderate, with the exception of lower molars, in which 49% had severe lesions. CONCLUSIONS: In this study, MIH lesions were asymmetrical both in presence and severity for all tooth types.


Asunto(s)
Hipoplasia del Esmalte Dental/epidemiología , Incisivo/patología , Maxilar/patología , Diente Molar/patología , Desmineralización Dental/epidemiología , Adolescente , Argentina/epidemiología , Niño , Hipoplasia del Esmalte Dental/clasificación , Femenino , Humanos , Masculino , Prevalencia , Índice de Severidad de la Enfermedad , Desmineralización Dental/clasificación
10.
J Vet Dent ; 36(1): 32-39, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31138050

RESUMEN

Mandibular fractures at the level of the first molar tooth (M1) were assessed in 29 dogs. Patients included in this study demonstrated fractures involving the M1 tooth, tooth bud, or alveolus (if tooth was absent). Diagnostic imaging evaluation included intraoral dental radiography and/or computed tomography (CT) with 3D reconstruction. The distal root was involved in 55.2% of cases, mesial root involvement in 34.5% of cases, and the tooth was absent in 10.3% of cases. Fractures were described in the rostral-to-caudal direction. Fractures tended to occur in the caudoventral direction ( P = .057). Cases with CT imaging were also evaluated in the buccolingual direction. Fractures were found to occur significantly more frequently in the caudolingual direction ( P = .022). When classifying fracture patterns along M1 according to a previously published fracture classification system, it was noted that fractures occurred significantly more frequently in either the mesial ( P < .001) or distal ( P < .001) roots by coursing along the periodontal ligament space and communicating with the periapical region. Active or nonworsening periodontitis was described as radiographic or tomographic evidence of (>25%) bone loss in the vertical or horizontal direction. Periodontitis was associated with 7 (24.1%) cases. These results help frame the challenges associated with fracture repair at the M1 location. Treatment planning considerations should include limited structural support caudal to fractures involving the distal root, more frequent involvement of the distal root over the mesial root, risk for poor endodontic prognosis, and the predilection for unfavorable fracture patterns to occur.


Asunto(s)
Enfermedades de los Perros/patología , Fracturas Mandibulares/veterinaria , Diente Molar/patología , Animales , Enfermedades de los Perros/diagnóstico por imagen , Perros , Fracturas Mandibulares/diagnóstico por imagen , Fracturas Mandibulares/patología , Diente Molar/diagnóstico por imagen , Radiografía Dental/veterinaria , Wisconsin
11.
BMJ Case Rep ; 12(4)2019 Apr 03.
Artículo en Inglés | MEDLINE | ID: mdl-30948389

RESUMEN

Mucoepidermoid carcinoma (MEC) can be rarely found as a primarily intraosseous lesion and mistaken for other intraosseous or odontogenic pathology. A 65-year-old man had a poorly defined radiolucency distal to the left mandibular second molar root. Periapical radiographs demonstrated a minor radiolucency from 2.5 years prior. An oral and maxillofacial surgeon felt the radiolucency represented periodontal disease, extracting tooth #18. The differential diagnosis of mixed radiolucent/radio-opaque mandibular lesions includes: (1) fibro-osseous lesion, (2) odontogenic and non-odontogenic cyst, (3) infection and inflammatory lesion, or (4) benign or malignant neoplasm (odontogenic, non-odontogenic, or metastatic). Histological analysis revealed low-grade MEC. A composite resection was performed with a 1 cm margin from first molar to ascending ramus. A buccal fat pad advancement flap covered the defect with an iliac crest bone graft placed later for a resulting osseous defect. Careful examination and diagnostic work-up for odontogenic cysts should be provided as they may harbour malignant tumours.


Asunto(s)
Carcinoma Mucoepidermoide/diagnóstico por imagen , Neoplasias Mandibulares/diagnóstico por imagen , Radiografía , Anciano , Carcinoma Mucoepidermoide/patología , Diagnóstico Diferencial , Humanos , Masculino , Mandíbula/diagnóstico por imagen , Mandíbula/patología , Neoplasias Mandibulares/patología , Diente Molar/diagnóstico por imagen , Diente Molar/patología , Quistes Odontogénicos/diagnóstico por imagen , Tumores Odontogénicos/diagnóstico por imagen
12.
Biomed Res Int ; 2019: 8528719, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30949512

RESUMEN

Aim: Cyclophilin A (CypA)/CD147 signaling plays critical roles in the regulation of inflammation and bone metabolism. This study aimed to investigate the participation of CypA/CD147 in mice periapical lesions progression and its relationship with bone resorption. Methodology: Periapical lesions were induced by pulp exposure in the first lower molars of 40 C57BL/6J mice. The mice were sacrificed on days 0, 7, 14, 21, 28, 35, 42, and 49. Mandibles were harvested for X-ray imaging, microcomputed tomography scanning, histologic observation, immunohistochemistry, enzyme histochemistry, and double immunofluorescence analysis. Western blot was employed to further detect the related molecular signaling pathways in LPS-stimulated RAW 264.7 cells treated with CypA inhibitor. Results: The volume and area of the periapical lesions increased from day 0 to day 35 and remained comparably stable until day 49. Immunohistochemistry demonstrated that the CypA expression levels also increased from day 0 to day 35 and decreased until day 49, similar to CD147 expression (R 2 = 0.4423, P < 0.05), osteoclast number (R 2 = 0.5101, P < 0.01), and the expression of osteoclastogenesis-related matrix metalloproteinase 9 (MMP-9) (R 2 = 0.4715, P < 0.05). Serial sections further confirmed the colocalization of CypA and CD147 on osteoclasts with immunohistochemistry. And the distribution of CypA-positive or CD147-positive cells was positively correlated with the dynamics of MMP-9-positive cells by using immunofluorescence analysis. Furthermore, CD147 and MMP-9 expression in RAW 264.7 cells were both downregulated with CypA inhibitor treatment (P < 0.05). Conclusions: The present study reveals the positive correlation of CypA/CD147 signaling and osteoclast-related MMP-9 expression in mice inflammatory periapical lesions progression. Therefore, intervention of CypA/CD147 signaling could probably provide a potential therapeutic target for attenuating inflammatory bone resorption.


Asunto(s)
Pérdida de Hueso Alveolar/metabolismo , Basigina/metabolismo , Ciclofilina A/metabolismo , Regulación Enzimológica de la Expresión Génica , Metaloproteinasa 9 de la Matriz/biosíntesis , Diente Molar/metabolismo , Transducción de Señal , Pérdida de Hueso Alveolar/inducido químicamente , Pérdida de Hueso Alveolar/patología , Animales , Lipopolisacáridos/toxicidad , Masculino , Ratones , Diente Molar/patología , Células RAW 264.7
13.
J Appl Biomater Funct Mater ; 17(1): 2280800019827798, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30808229

RESUMEN

Nowadays, dental caries is one of the most common oral health problems, affecting most individuals. It has been found that, by remineralizing enamel at an early stage in the formation of enamel caries, teeth can be effectively protected from dental caries. In this work, a peptide with eight repetitive sequences of aspartate-serine-serine (8DSS) is applied as the bio-mineralizer in an in-vivo rat enamel caries model. Nondestructive quantitative light-induced fluorescence-digital (QLF-D) imaging and micro-computed tomography (micro-CT) are used to evaluate the remineralization of enamel carious lesions by measuring the total fluorescence radiance loss of the molar area (Δ QTotal), acquired using QLF-D imaging, and the mineral density and residual molar enamel volume, acquired using micro-CT. Correlations are explored between Δ QTotal and mineral density (strong correlation, r = 0.8000, p < 0.001) and Δ QTotal and residual molar enamel volume (moderate correlation, r = 0.6375, p < 0.001). Our results demonstrate that 8DSS is a promising in-vivo remineralization agent that exhibits comparable effects to NaF ( p < 0.05), which has been verified using the classical Keyes method. Moreover, the nondestructive QLF-D and micro-CT methods can be combined to quantify the remineralization of enamel carious lesions three-dimensionally in vivo, making them broadly applicable in quantifying hard tissues.


Asunto(s)
Diente Molar/efectos de los fármacos , Péptidos/farmacocinética , Remineralización Dental/métodos , Secuencia de Aminoácidos , Animales , Caries Dental/tratamiento farmacológico , Caries Dental/patología , Modelos Animales de Enfermedad , Diente Molar/diagnóstico por imagen , Diente Molar/patología , Péptidos/química , Péptidos/farmacología , Péptidos/uso terapéutico , Fluorescencia Cuantitativa Inducida por la Luz , Ratas , Índice de Severidad de la Enfermedad , Microtomografía por Rayos X
14.
PLoS One ; 14(2): e0211928, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30735554

RESUMEN

Osteonecrosis of the jaw induced by administration of bisphosphonates (BPs), BP-related osteonecrosis (BRONJ), typically develops after tooth extraction and is medically challenging. As BPs inhibit oral mucosal cell growth, we hypothesized that suppression of the wound healing-inhibiting effects could prevent BRONJ onset after tooth extraction. Since basic fibroblast growth factor (bFGF) promotes wound healing, but has a short half-life, we examined whether the initiation of BRONJ could be prevented by applying a bFGF-containing gelatin hydrogel over the extraction sockets of BRONJ model rats. Forty-three rats, received two intravenous injections of zoledronic acid 60 µg/kg, once per week for a period of 2 weeks, underwent extraction of a unilateral lower first molar. The rats here were randomly assigned to the bFGF group (n = 15 rats, gelatin hydrogel sheets with incorporated bFGF applied over the sockets); the phosphate-buffered saline (PBS) group (n = 14 rats, gelatin hydrogel sheets without bFGF applied over the sockets); or the control group (n = 14 rats, nothing applied over the sockets). One rat in the bFGF group was sacrificed immediately after tooth extraction. Twenty-one rats were sacrificed at 3 weeks, and the remaining 21 rats were sacrificed at 8 weeks after tooth extractions. The harvested mandibles were analyzed using micro-computed tomography and sections were evaluated qualitatively for mucosal disruption and osteonecrosis. The incidence of osteonecrosis at 8 weeks after tooth extraction was 0% in the bFGF group, 100% in the PBS group, and 85.7% in the control group. The frequency of complete coverage of the extraction socket by mucosal tissue was significantly greater in the bFGF group than in the other groups. These results suggest that application of bFGF in the extraction socket promoted socket healing, which prevented BRONJ development. The growth-stimulating effects of bFGF may have offset the inhibition of wound healing by BP.


Asunto(s)
Preparaciones de Acción Retardada/química , Factor 2 de Crecimiento de Fibroblastos/farmacología , Mucosa Bucal/efectos de los fármacos , Extracción Dental/efectos adversos , Cicatrización de Heridas/efectos de los fármacos , Animales , Osteonecrosis de los Maxilares Asociada a Difosfonatos/diagnóstico por imagen , Osteonecrosis de los Maxilares Asociada a Difosfonatos/tratamiento farmacológico , Osteonecrosis de los Maxilares Asociada a Difosfonatos/etiología , Osteonecrosis de los Maxilares Asociada a Difosfonatos/patología , Femenino , Factor 2 de Crecimiento de Fibroblastos/farmacocinética , Gelatina/química , Humanos , Hidrogeles/química , Mandíbula/diagnóstico por imagen , Mandíbula/efectos de los fármacos , Mandíbula/patología , Diente Molar/efectos de los fármacos , Diente Molar/patología , Diente Molar/cirugía , Mucosa Bucal/diagnóstico por imagen , Mucosa Bucal/patología , Ratas , Ratas Sprague-Dawley , Alveolo Dental/diagnóstico por imagen , Alveolo Dental/efectos de los fármacos , Alveolo Dental/patología , Microtomografía por Rayos X , Ácido Zoledrónico/administración & dosificación
15.
Clin Oral Investig ; 23(10): 3789-3799, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-30759283

RESUMEN

OBJECTIVES: The aim of this randomized, three-arm parallel, single-blinded clinical trial was to evaluate the clinical and microbiological effectiveness of the ozone application in two-visit indirect pulp therapy. MATERIALS AND METHODS: One hundred five lower first molar teeth with deep caries lesion were included and randomly assigned three groups to apply the two-visit indirect pulp therapy. Treatment procedure was applied without any disinfectant (control), with 60-s 2% chlorhexidine digluconate (CHX) or 60-s ozone application. In four different stages (after initial excavation, ozone/CHX application before the temporary restoration, 4 months later immediately after removing temporary restoration, and final excavation), dentin humidity, consistency, and color properties were recorded to evaluate the clinical characteristics of the tissue, and standard dentin samples were collected for the microbiological analysis of mutans streptococci, lactobacilli, and the total number of colony-forming units. The data were analyzed by using Mann-Whitney U test for multiple comparisons. RESULTS: The remaining dentin became harder, drier, and darker after 4 months in all groups. However, CHX and ozone application were statistically better than the control group (p < 0.05). There was a gradual decrease in the total number of microorganisms in all groups. While cavity disinfectant applications were improved the antibacterial efficacy (control, 79.11%; CHX, 98.39%; ozone, 93.33%), CHX application exhibited a greater significant reduction than both groups (p = 0.000). CONCLUSION: The two-visit indirect pulp therapy yielded successful results for all study groups. However, CHX would be conveniently preferable due to improving the treatment success. CLINICAL RELEVANCE: The two-visit indirect pulp therapy applied with cavity disinfectant is a proper alternative treatment procedure in deep carious lesions, instead of conventional technique.


Asunto(s)
Caries Dental/terapia , Desinfectantes Dentales/uso terapéutico , Diente Molar/patología , Ozono/uso terapéutico , Exposición de la Pulpa Dental , Dentina , Humanos , Streptococcus mutans
16.
J Indian Soc Pedod Prev Dent ; 37(1): 107-109, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30804316

RESUMEN

Teeth which erupt in the 1st month of postnatal life are known as "neonatal tooth." The incidence of these teeth ranges from 1:2000 to 1:3500 live births. Natal teeth are more common in mandibular central incisor region, followed by maxillary incisor region and mandibular canine region. The neonatal or natal teeth in the maxillary molar region are a rare occurrence. This article represents a rare case of the neonatal tooth with Langerhans cell histiocytosis.


Asunto(s)
Histiocitosis de Células de Langerhans/patología , Diente Molar/anomalías , Erupción Dental , Humanos , Lactante , Masculino , Maxilar/anomalías , Diente Molar/crecimiento & desarrollo , Diente Molar/patología
17.
Lasers Med Sci ; 34(6): 1235-1241, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-30673922

RESUMEN

This in vitro study aimed to compare the performance of a light-emitting diode (LED) device (Midwest Caries I.D.: MID), International Caries Detection and Assessment System (ICDAS) visual criteria, and fluorescence-based devices (DIAGNOdent: LF; DIAGNOdent pen: LFpen; and Quantitative Light-induced Fluorescence: QLF) in detecting occlusal caries in the primary molars. Eighty-eight primary molars with sound occlusal surfaces or carious lesions at different stages were assessed twice, with a 1-week interval in between, by one examiner using all three methods. Subsequently, the teeth were sectioned and lesion depth was verified using stereomicroscopy as a gold standard. Sensitivity, specificity, and accuracy were calculated at D1 (all carious lesions-enamel and dentin) and D3 (dentin lesions) thresholds. Correlation with histological analysis was evaluated using Spearman's rank correlation coefficients (rho). Weighted Kappa and intraclass-correlation (ICC) coefficients were calculated to assess intra-examiner reproducibility. At D1 threshold, ICDAS and LFpen showed higher sensitivity than the other methods, whereas ICDAS, LF, and QLF showed higher specificity (p < 0.05), and MID showed lower accuracy. At D3 threshold, ICDAS, LFpen, and QLF showed higher sensitivity than MID, whereas ICDAS, LF, and MID showed higher specificity (p < 0.05). All methods, except MID, showed statistically similar accuracy values (p < 0.05). Correlations with histopathological analysis varied from 0.15 (MID) to 0.57 (ICDAS). Intra-examiner reproducibility varied from 0.30 (MID) to 0.92 (ICDAS, LF, and QLF). The MID device exhibited a poor performance in detecting occlusal carious lesions in the primary molars, and ICDAS visual criteria exhibited greater accuracy than LF, LFpen, and QLF devices.


Asunto(s)
Caries Dental/diagnóstico , Oclusión Dental , Luz , Diente Molar/patología , Humanos , Sensibilidad y Especificidad , Estadísticas no Paramétricas
18.
Mol Genet Metab ; 126(4): 504-512, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30691926

RESUMEN

Mutations of the TRPS1 gene cause trichorhinophalangeal syndrome (TRPS), a skeletal dysplasia with dental abnormalities. TRPS dental phenotypes suggest that TRPS1 regulates multiple aspects of odontogenesis, including the tooth number and size. Previous studies delineating Trps1 expression throughout embryonic tooth development in mice detected strong Trps1 expression in dental mesenchyme, preodontoblasts, and dental follicles, suggesting that TRPS dental phenotypes result from abnormalities in early developmental processes. In this study, Trps1+/- and Trps1-/- mice were analyzed to determine consequences of Trps1 deficiency on odontogenesis. We focused on the aspects of tooth formation that are disturbed in TRPS and on potential molecular abnormalities underlying TRPS dental phenotypes. Microcomputed tomography analyses of molars were used to determine tooth size, crown shape, and mineralization of dental tissues. These analyses uncovered that disruption of one Trps1 allele is sufficient to impair mineralization of dentin in both male and female mice. Enamel mineral density was decreased only in males, while mineralization of the root dental tissues was decreased only in females. In addition, significantly smaller teeth were detected in Trps1+/- females. Histomorphometric analyses of tooth organs showed reduced anterior-posterior diameter in Trps1-/- mice. BrdU-incorporation assay detected reduced proliferation of mesenchymal and epithelial cells in Trps1-/- tooth organs. Immunohistochemistry for Runx2 and Osx osteogenic transcription factors revealed changes in their spatial distribution in Trps1-/- tooth organs and uncovered cell-type specific requirements of Trps1 for Osx expression. In conclusion, this study has demonstrated that Trps1 is a positive regulator of cell proliferation in both dental mesenchyme and epithelium, suggesting that the microdontia in TRPS is likely due to decreased cell proliferation in developing tooth organs. Furthermore, the reduced mineralization observed in Trps1+/- mice may provide some explanation for the extensive dental caries reported in TRPS patients.


Asunto(s)
Proliferación Celular , Factores de Transcripción GATA/genética , Regulación de la Expresión Génica , Odontogénesis , Calcificación de Dientes , Alelos , Animales , Diferenciación Celular , Caries Dental/etiología , Células Epiteliales , Femenino , Dedos/anomalías , Enfermedades del Cabello/complicaciones , Enfermedades del Cabello/genética , Síndrome de Langer-Giedion/complicaciones , Síndrome de Langer-Giedion/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Diente Molar/patología , Nariz/anomalías , Microtomografía por Rayos X
19.
Photodiagnosis Photodyn Ther ; 25: 265-270, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30611863

RESUMEN

BACKGROUND: The difference in autofluorescence between enamel and dentine layer has prompted recommendations to use the quantitative light-induced fluorescence (QLF) method for quantifying tooth wear (TW). This study investigated the potential of QLF for distinguishing the severity of occlusal TW based on differences in the autofluorescence intensity. METHODS: In total, 106 extracted permanent molars and premolars having suspected wear without pulp exposure were used. The severity of wear was determined by visually examining all teeth using the tooth wear index (TWI) of Smith and Knight. QLF images were captured and converted into 8-bit grayscale images. The difference in the fluorescence intensity (ΔG) was calculated by comparing mean grayscale levels between sound and worn areas. Finally, histological examination was conducted by stereomicroscope to confirm the presence of dentine exposure. RESULTS: 100 teeth were included in the final analysis without six teeth having enamel cracks around worn area. The ΔG values increased with the severity of TW as quantified using conventional TWI codes, and differed significantly between the sound and enamel- and dentine-wear teeth (P < 0.001). The histology indicated that enamel remained on 57 teeth, while 43 teeth had dentine-exposed wear and showed significant differences in ΔG compared with enamel-remained teeth. CONCLUSIONS: The fluorescence intensity differed significantly depending on the presence of dentine exposure. ΔG could be used to distinguish between sound and enamel- and dentine-wear teeth with a significant correlation. These findings indicate that QLF could be useful for determining the severity of TW of occlusal surfaces noninvasively.


Asunto(s)
Esmalte Dental/patología , Dentina/patología , Fluorescencia Cuantitativa Inducida por la Luz/métodos , Desgaste de los Dientes/diagnóstico , Diente Premolar , Humanos , Diente Molar/patología
20.
Photodiagnosis Photodyn Ther ; 25: 142-147, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30508664

RESUMEN

BACKGROUND: This study compared two fluorescence parameters (fluorescence loss [ΔF] and red fluorescence gain [ΔR]) among three generations of quantitative light-induced fluorescence (QLF) systems with the aim of determining the validities of these parameters in the three devices for differentiating the severity of enamel caries. METHODS: Forty-one extracted human premolars and molars with suspected enamel caries were selected. Fluorescence images of all teeth were obtained using first-, second-, and third-generation QLF systems (Inspektor Pro, QLF-D, and Qraycam, respectively). Fluorescence parameters were then calculated using proprietary software. All of the specimens were also categorized histologically using polarized-light microscopy (PLM) based on histological levels related to the lesion depth into sound enamel (S), caries limited to the outer half of the enamel (E1), and caries involving the inner half of the enamel (E2). The Mann-Whitney test with Bonferroni correction was used to compare fluorescence parameters among the three generations of systems. The sensitivity, specificity, and area under the receiver operating characteristics curve (AUC) at two thresholds (S/E1 for detecting enamel caries lesions and E1/E2 for differentiating the caries severity) were calculated for evaluating the validities of the fluorescence parameters obtained using all three generations of QLF devices. RESULTS: ΔF did not differ significantly between the devices at any histological level. In addition, ΔF showed large AUCs at the thresholds of S/E1 and E1/E2 (0.97-0.98 and 0.89-0.90, respectively). On the other hand, ΔR was significantly higher for the third-generation device than for the first- and second-generation devices for E2 lesions (P < 0.001). At the S/E1 threshold, ΔR values of the first- and third-generation devices showed larger AUCs (0.96-0.97) compared with that of the second-generation device (0.91), whereas at the E1/E2 threshold the AUC was the largest for the third-generation device (0.87). CONCLUSIONS: The ΔF fluorescence parameter did not differ between the three generations of QLF devices, and showed high validity values. In terms of ΔR, the devices of all generations also showed good diagnostic performance for quantifying and detecting enamel caries lesions, but the third-generation QLF system produced superior results.


Asunto(s)
Caries Dental/diagnóstico , Caries Dental/patología , Esmalte Dental/patología , Fluorescencia Cuantitativa Inducida por la Luz/instrumentación , Diente Premolar/patología , Humanos , Diente Molar/patología , Fluorescencia Cuantitativa Inducida por la Luz/normas
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