Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 18.015
Filtrar
1.
Science ; 371(6535)2021 03 19.
Artículo en Inglés | MEDLINE | ID: mdl-33737460

RESUMEN

The intestine is a site of direct encounter with the external environment and must consequently balance barrier defense with nutrient uptake. To investigate how nutrient uptake is regulated in the small intestine, we tested the effect of diets with different macronutrient compositions on epithelial gene expression. We found that enzymes and transporters required for carbohydrate digestion and absorption were regulated by carbohydrate availability. The "on-demand" induction of this machinery required γδ T cells, which regulated this program through the suppression of interleukin-22 production by type 3 innate lymphoid cells. Nutrient availability altered the tissue localization and transcriptome of γδ T cells. Additionally, transcriptional responses to diet involved cellular remodeling of the epithelial compartment. Thus, this work identifies a role for γδ T cells in nutrient sensing.


Asunto(s)
Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Enterocitos/fisiología , Interleucinas/metabolismo , Mucosa Intestinal/fisiología , Receptores de Antígenos de Linfocitos T gamma-delta , Subgrupos de Linfocitos T/fisiología , Adaptación Fisiológica , Animales , Comunicación Celular , Proteínas en la Dieta/administración & dosificación , Digestión , Regulación de la Expresión Génica , Interleucinas/genética , Absorción Intestinal , Mucosa Intestinal/citología , Intestino Delgado/citología , Intestino Delgado/metabolismo , Ratones Endogámicos C57BL , Nutrientes/administración & dosificación , Nutrientes/metabolismo , Subgrupos de Linfocitos T/inmunología , Transcripción Genética , Transcriptoma
2.
Adv Exp Med Biol ; 1285: 29-42, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33770401

RESUMEN

Proteins have been recognized for a long time as an important dietary nutritional component for all animals. Most amino acids were isolated and characterized in the late nineteenth and early twentieth century. Initially dietary proteins were ranked high to low quality by growth and N balance studies. By the 1950s interest had shifted to studying the roles of individual amino acids in amino acid requirements by feeding studies with non-ruminants as rodents, poultry and pigs. The direct protein feeding approaches followed by measurements of nutritional outcomes were not possible however in ruminants (cattle and sheep). The development of measuring free amino acids by ion exchange chromatography enabled plasma amino acid analysis. It was thought that plasma amino acid profiles were useful in nutritional studies on proteins and amino acids. With non-ruminants, nutritional interpretations of plasma amino acid studies were possible. Unfortunately with beef cattle, protein/amino acid nutritional adequacy or requirements could not be routinely determined with plasma amino acid studies. In dairy cows, however, much valuable understanding was gained from amino acid studies. Concurrently, others studied amino acid transport in ruminant small intestines, the role of peptides in ruminant N metabolism, amino acid catabolism (in the animal) with emphasis on branched-chain amino acid catabolism. In addition, workable methodologies for studying protein turnover in ruminants were developed. By the 1990s, nutritionists could still not determine amino acid requirements with empirical experimental studies in beef cattle. Instead, computer software (expert systems) based on the accumulated knowledge in animal and ruminal amino acids, energy metabolism and protein production were realized and revised frequently. With these tools, the amino acid requirements, daily energy needs, ruminal and total gastrointestinal tract digestion and performance of growing beef cattle could be predicted.


Asunto(s)
Aminoácidos , Alimentación Animal , Alimentación Animal/análisis , Animales , Bovinos , Dieta , Proteínas en la Dieta , Digestión , Femenino , Ovinos , Porcinos
3.
Water Res ; 194: 116962, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33657493

RESUMEN

Anaerobic digestion is a commonly used process for the reduction and stabilization of wasted activated sludge generated in wastewater treatment plants. However, anaerobically-digested (AD) sludge is still a problematic waste stream due to its large volume and often poor quality. In this study, two aerobic digesters were set up to treat anaerobically-digested sludge, with one digester operated in self-generated acidic condition as the experimental reactor, and one at neutral pH as the control reactor. The acidic condition in the experimental reactor was driven by an inoculated special ammonia-oxidizing bacterium, 'Candidatus Nitrosoglobus', which can tolerate low pH. As a result of ammonium oxidation by Ca. Nitrosoglobus, the pH decreased to 4.8 ± 0.2 and nitrite accumulated to and stayed at 200.0 ± 17.2 mg N L-1, from which free nitrous acid (FNA) at 8.5 ± 1.8 mg HNO2N L-1 formed in-situ. As a combined effect of low pH and high concentration of FNA, the experimental reactor reduced the total solids (TS), volatile solids (VS) and non-volatile solids (NVS) in the AD sludge by 25.2 ± 7.0%, 29.8 ± 4.3%, and 22.6 ± 5.5%, respectively. In contrast, the control reactor without Ca. Nitrosoglobus inoculation (operated at a near-neutral pH of 6.8 ± 0.3 and no FNA formation) only reduced VS in the AD sludge by 10.4 ± 4.3%, along with negligible NVS reduction. Additionally, the acidic aerobic digestion in the experimental reactor significantly stabilized AD sludge, decreasing the specific oxygen uptake rate (SOUR) to 0.5 ± 0.1 mg O2 g-1VS h-1 and the most probable number (MPN) of Faecal Coliforms to 2.4 ± 0.1 log(MPN g-1TS), both of which meet USEPA standards for Class A biosolids. In comparison, the control reactor produced biosolids at Class B level only, with an SOUR of 1.8 ± 0.2 mg O2 g-1VS h-1 and a Faecal Coliforms MPN of 3.6 ± 0.1 log(MPN g-1TS). By reducing the volume and improving the quality of the AD sludge, the acidic aerobic digestion of AD sludge enabled by Ca. Nitrosoglobus has the potential to significantly save the sludge disposal costs in wastewater treatment.


Asunto(s)
Amoníaco , Aguas del Alcantarillado , Anaerobiosis , Reactores Biológicos , Digestión , Oxidación-Reducción , Eliminación de Residuos Líquidos
4.
Food Chem ; 352: 129332, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33690075

RESUMEN

In order to construct a novel and efficient calcium delivery system, a dextran- casein phosphopeptide (CPP) conjugates as calcium carrier was prepared by Maillard reaction of CPP and dextran. The preparation of the conjugates, construction of calcium delivery system and digestion in vitro were studied. The grafting rate of conjugates, which was confirmed by migration and intensity changes in the characteristic peaks using ultraviolet-visible and Fourier transform infrared spectroscopy, reached 48.88%. The microscopy showed CPP was coated with dextran, the conjugates with a kind of "shell-core" structure had excellent stability. Compared with CPP, the chelating rate of conjugates increased from 6.0% to 13.87%, and the calcium retention rate improved from 1.09% to 7.90% in vitro digestion. The calcium binding capacity and effect of controlled release of the conjugates were superior to those of CPP. Therefore, the conjugates could be used as an effective carrier for new calcium supplements.


Asunto(s)
Calcio/química , Caseínas/química , Dextranos/química , Digestión , Portadores de Fármacos/química , Fosfopéptidos/química , Calcio/metabolismo , Reacción de Maillard
5.
Food Chem ; 351: 129303, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33647689

RESUMEN

Herein, we investigated the impact of moist (steaming and boiling) and dry (baking and microwaving)-heat treatment processes on the structure and physicochemical properties of wheat starch (WS) supplemented with lauric acid (LA). Elemental composition analysis revealed the interplay between WS and LA. Scanning electron microscopy (SEM) and iodine staining revealed that lamellar crystalline structure of WS-LA complexes was improved after moist-heat treatment (relative to samples without any heat treatments); the finding which is at variance to dry-heat treatment process. Additionally, high resistance to thermal decomposition and a lower 1022/995 cm-1 absorbance ratio were observed in moist-heat treated WS-LA compared with dry-heat samples. Moreover, the V-type diffraction peak intensity and resistance to in vitro enzymatic hydrolysis of samples treated with moist-heat were increased to a greater extent than the dry-heat treated counterparts. In sum, this study would facilitate the application of functional starch-lipid complexes in food necessitated heat treatments.


Asunto(s)
Calor , Ácidos Láuricos/química , Almidón/química , Triticum/química , Digestión , Manipulación de Alimentos , Hidrólisis , Microscopía Electrónica de Rastreo , Almidón/metabolismo
6.
Food Chem ; 352: 129398, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33652197

RESUMEN

We investigated the effects of ultrasonic treatment (400 W, 20 kHz, 45.52 W/L) and storage time (0 d, 3 d, 7 d and 10 d) on functional properties, structural changes and in vitro digestion of actomyosin complex isolated from vacuum-packed pork. As storage time increased, turbidity, surface hydrophobicity, active sulfhydryl and total sulfhydryl of actomyosin complex increased, while protein solubility decreased. Ultrasonic treatment increased surface hydrophobicity, protein solubility and active sulfhydryl content but decreased turbidity and total sulfhydryl content compared with the control. Ultrasonic treatment caused a reduction in α-helix content on 0 day and the fluorescence intensity of tryptophan and tyrosine residues. It increased pancreatin digestibility of actomyosin complex and the number of peptides of smaller than 1 kDa. However, it decreased the number of peptides. The findings provide a new insight into the application of appropriate ultrasonic treatment to promote meat digestibility.


Asunto(s)
Actomiosina/química , Digestión , Almacenamiento de Alimentos , Carne/análisis , Ondas Ultrasónicas , Actomiosina/metabolismo , Animales , Interacciones Hidrofóbicas e Hidrofílicas , Solubilidad , Compuestos de Sulfhidrilo/química , Porcinos
7.
Food Chem ; 352: 129400, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33691213

RESUMEN

The microbial transglutaminase (mTG) was used to improve the stability of the naringenin-loaded ß-casein micelles (CNMs). The formation of cross-linked CNMs was confirmed by SDS-PAGE electrophoresis, showing a decrease in monomeric ß-CN levels with increasing crosslinking time. Dynamic light scattering (DLS) showed that after crosslinking the particle size distribution did not change upon dilution, suggesting occurrence of intra-crosslinking. Fluorescence spectroscopy and circular dichroism (CD) showed that crosslinking induced only minor changes in the structure. Finally, release of naringenin in buffer at pH 7.4 demonstrated a slower release from the cross-linked micelles compared to the untreated micelles. In addition, the cross-linked micelles exhibited a partial resistance to pepsin enzyme. We conclude that crosslinking with mTG is a suitable method to modulate naringenin release kinetics from ß-CN micelles and improves the potential of these micelles as delivery systems targeted to the small intestine.


Asunto(s)
Caseínas/química , Digestión , Portadores de Fármacos/química , Liberación de Fármacos , Flavanonas/química , Micelas , Transglutaminasas/metabolismo , Concentración de Iones de Hidrógeno
8.
Food Chem ; 352: 129456, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33711727

RESUMEN

Release of bioelements and phenolic compounds from edible mushrooms (Agaricus bisporus, Cantharellus cibarius, and Lentinula edodes) enriched with zinc, selenium, l-phenylalanine, alone and as a mixture was examined using a simulated human gastrointestinal digestion method. Due to the extensive amount of data obtained, in order to interpret them more precisely in the work, the methods of chemometric analysis (Cluster Analysis-CA and Principal Compenent Analysis-PCA) were additionally applied. The results showed mycelium of L. edodes has the best health-promoting properties and addition of mixture to the media increased significantly the synthesis of p-hydroxybenzoic and protocatechuic acid (267 and 16.3 mg/100 g d.w.). After extraction into artificial digestive juices, 97.4 mg/100 g d.w. p-hydroxybenzoic acid and 15.6 mg/100 g d.w. of protocatechuic acid were released. The greatest amounts of Se and Zn were extracted from enriched A. bisporus mycelium (32.3 and 342 mg/100 g d.w., respectively). This study confirmed that mycelium might prevent nutritional deficiencies in the diet through use of functional foods.


Asunto(s)
Agaricus/química , Basidiomycota/química , Micelio/química , Fenoles/análisis , Hongos Shiitake/química , Disponibilidad Biológica , Digestión , Humanos , Fenoles/metabolismo
9.
Carbohydr Polym ; 260: 117801, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712149

RESUMEN

Slowly digestible starches have received interest due to their lower increase of postprandial blood glucose and insulin levels and, hence, modification of starches towards slower digestibility has commercial interest. However, chemical characteristics driving enzymatic (digestive) degradation are not fully unraveled. The digestion properties of starches have been linked to their crystalline type, chain length distribution, amylose content or degree of branching, but content and length of relatively long side-chains in amylopectin has not been paid attention to. Therefore, this research focusses on the unique content and length of amylopectin side-chains from conventional and new starch sources (potato, corn, pea, and tulip) correlated to the enzymatic digestion. The rate of hydrolysis was found to be correlated with the crystalline type of starch, as previously suggested, however, the complete hydrolysis of all starches, independent of the crystalline type and source, was shown to be governed by the content of longer amylopectin chains.


Asunto(s)
Gelatina/química , Glucano 1,4-alfa-Glucosidasa/metabolismo , Almidón/metabolismo , alfa-Amilasas/metabolismo , Cristalización , Digestión , Hidrólisis , Espectroscopía de Resonancia Magnética , Guisantes/metabolismo , Solanum tuberosum/metabolismo , Almidón/química , Zea mays/metabolismo
10.
Food Chem ; 351: 129315, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33647686

RESUMEN

Mangiferin-loaded nanobilosomes (MGF-NBSs) were developed using microfluidic-based techniques to improve aqueous solubility, digestive stability, and cellular antioxidant activity (CAA) of mangiferin. Preliminary experiments showed that optimal formation conditions were 5:1 aqueous (water) to solvent (ethanol) phase ratio and 85 mL/min total flow rate. Further optimization using response surface methodology provided the optimal formulation (200 mg encapsulant consisting of 90.91% phosphatidylcholine and 9.09% sodium glycocholate, and 25.89 mg mangiferin), achieving 9.25% mangiferin loading and 80.65% encapsulation efficiency. Mono-dispersed MGF-NBSs with an average size of around 48.14 nm and zeta potential of -30.1 mV were obtained. FTIR and DSC results confirmed the successful encapsulation of mangiferin into the nanobilosomes and revealed interactions among the components. MGF-NBSs showed a 7-fold increase in the aqueous solubility compared with non-encapsulated mangiferin. CAA of MGF-NBSs in Caco-2 cells was 2 times higher than that of mangiferin and the in vitro digestive stability was improved.


Asunto(s)
Antioxidantes/química , Antioxidantes/farmacología , Nanoestructuras/química , Agua/química , Xantonas/química , Xantonas/farmacología , Antioxidantes/metabolismo , Células CACO-2 , Digestión , Composición de Medicamentos , Humanos , Dispositivos Laboratorio en un Chip , Tamaño de la Partícula , Solubilidad , Xantonas/metabolismo
11.
Food Chem ; 351: 129320, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33662906

RESUMEN

In this study, mushroom stems were separated from the fruiting body of two edible mushrooms, white button mushroom (WB, Agaricus bisporus) and oyster mushroom (OY, Pleurotus ostreatus), and their functionalities were compared in wheat flour noodles at fortification levels of 5, 10, 15%. The inclusion of WB led to higher protein content than OY, which had more dietary fibre, especially insoluble dietary fibre. The fortification of mushrooms decreased the area under the curve (AUC) of reducing sugars released during in vitro digestion significantly (p < 0.05). WB fortified noodles yielded higher antioxidant capacities than OY fortification, whereas the digesta following digestion of WB and OY groups shared similar free accessible weighted average antioxidants. Mushrooms derived insoluble dietary fibre was negatively correlated with AUC and positively correlated with antioxidants (p < 0.05), suggesting the efficacy of mushroom stems over post-prandial glucose release of foods and providing the antioxidant environment to the intestine.


Asunto(s)
Agaricus/química , Antioxidantes/metabolismo , Antioxidantes/farmacología , Fibras de la Dieta/metabolismo , Fibras de la Dieta/farmacología , Digestión , Carga Glucémica/efectos de los fármacos
12.
Medicine (Baltimore) ; 100(9): e24777, 2021 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-33655941

RESUMEN

INTRODUCTION: Knee osteoarthritis is a common condition that affects daily functioning and decreases the quality of life. There are many ways of treatment depending on the stage of the disease. Advanced cases are qualified for arthroplasty, which is an extensive and demanding surgical procedure. Less advanced stages are treated in various ways: from rehabilitation, through oral and intra-articular pharmacotherapy, to surgical treatment (arthroscopy, osteotomy). Because surgical treatment is risky, scientists focus on less invasive therapeutic methods. The most valuable management is based on regeneration. Mesenchymal stromal cells (MSC) derived from the adipose tissue have a great regenerative and anti-inflammatory potential, therefore an attempt is being made to take advantage of them in knee osteoarthritis treatment.The study aims to compare the clinical effects of treatment of knee osteoarthritis using adipose tissue MSC obtained by an enzymatic method with the outcomes of the therapy with the mechanically fragmented adipose tissue. METHODS: One hundred adults with primary knee osteoarthritis will undergo lipoaspiration under sterile conditions. The collected lipoaspirates will be further processed, depending on the randomly assigned group-enzymatically with the use of collagenase or mechanically using the Lipogems system. The preparations will be administered to the patients' knee joints in the operating room under ultrasound control.The results of treatment will be assessed using Knee Injury and Osteoarthritis Outcome Score, measuring the flexibility of the knee joint, evaluating joint gap in X-ray and the quality of cartilage in magnetic resonance T2-mapping during 1 year after treatment. DISCUSSION/CONCLUSION: Identification and functional analysis of the regenerative capacity of adipose-derived MSC depending on three variables (body weight, sex, and age) will help to develop a targeted therapy for different groups of patients and will determine the effectiveness of both methods of treatment. An attempt will be made to identify groups of patients with the greatest regenerative potential of the adipose tissue, and thus indicate those with the most probable improvement of the joint condition. TRIAL REGISTRATION: This study protocol has been approved by the Ethics Committee of Medical University of Warsaw and registered on www.clinicaltrials.gov: NCT04675359 (06 Jan 2021).


Asunto(s)
Tejido Adiposo/citología , Separación Celular/métodos , Trasplante de Células Madre Mesenquimatosas/métodos , Células Madre Mesenquimatosas/citología , Osteoartritis de la Rodilla/cirugía , Adulto , Técnicas de Cultivo de Célula , Colagenasas , Digestión/fisiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Medición de Resultados Informados por el Paciente , Ensayos Clínicos Controlados Aleatorios como Asunto , Estrés Mecánico , Resultado del Tratamiento
13.
Bioresour Technol ; 329: 124919, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33676353

RESUMEN

Links between synergy and microbial community characteristics in co-digestion of food waste (FW), cattle manure (CM) and corn straw (CS) were investigated. Mono-digestion of FW and CS were inhibited by organic acids. Co-digestion of FW with CM achieved greater synergistic rates (18.5% and 22.3%) than CM with CS (14.8% and 12.3%). Synergy resulted from coupling effects of improving nutrient balance, dilution of toxic compounds, higher buffering capacity, detoxification based on co-metabolism, which ultimately reflected in microbial community functions. Although co-digestion of FW with CS exhibited lowest synergistic rates (7.9% and 4.9%), detoxification based on co-metabolism of syntrophic communities of Syntrophomonadaceae with hydrogenotrophic methanogens accelerated system recovery. Digester with the greatest synergy (65% FW + 35% CM) maintained dominant growth of hydrogenotrophic methanogens (68.9%), highest methanogenic community diversity and relative abundance of Methanosarcina (14.6%), which sustained more diverse and switchable methanogenic pathways therefore ensured powerful methanogenic functions and vigorous methanogenic capability.


Asunto(s)
Microbiota , Eliminación de Residuos , Anaerobiosis , Animales , Biocombustibles , Reactores Biológicos , Bovinos , Digestión , Alimentos , Estiércol , Metano , Zea mays
14.
Bioresour Technol ; 330: 124970, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33735726

RESUMEN

Currently, anaerobic sludge digestion (ASD) is considered not only for treating residual sewage sludge and energy recovery but also for the reduction of antibiotic resistance genes (ARGs). The current review highlights the reasons why antibiotic resistant bacteria (ARB) and ARGs exist in ASD and how ASD performs in the reduction of ARB and ARGs. ARGs and ARB have been detected in ASD with some reports indicating some of the ARGs can be completely removed during the ASD process, while other studies reported the enrichment of ARB and ARGs after ASD. This paper reviews the performance of ASD based on operational parameters as well as environmental chemistry. More studies are needed to improve the performance of ASD in reducing ARGs that are difficult to handle and also differentiate between extracellular (eARGs) and intracellular ARGs (iARGs) to achieve more accurate quantification of the ARGs.


Asunto(s)
Genes Bacterianos , Aguas del Alcantarillado , Anaerobiosis , Antagonistas de Receptores de Angiotensina , Inhibidores de la Enzima Convertidora de Angiotensina , Antibacterianos/farmacología , Bacterias/genética , Digestión , Farmacorresistencia Microbiana/genética , Aguas Residuales
15.
Bioresour Technol ; 330: 124951, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33735734

RESUMEN

High solid anaerobic digestion (HSAD) was an emerging bioconversion technology which had the advantages of small digester, less digestate and low heating energy. A one-stage anaerobic system in CSTR by inoculating activated sludge of simultaneous propionate degradation and methanogenesis was proposed to improve the high-solid digestion performance and to stabilize the reaction process. Semi-continuous mode was successfully used to perform HSAD from cellulosic ethanol whole stillage at an initial substrate loading of 15.4% (w/w) dry matter content with different OLRs from 1.5 to 5.0 gVS·L-1 d-1 at an HRT of 30 days. The average methane yield during whole digestion reached 349.9 mL⋅gVS-1 with a total VS removal rate of 61.3%. The acclimation mechanism of multifunctional activated sludge was also explored by analyzing the functional property, physiological activity and microbial community structure. The results indicated the feasibility and efficiency of multifunctional activated sludge in a semi-continuous high-solid stirred tank reactor system.


Asunto(s)
Propionatos , Aguas del Alcantarillado , Anaerobiosis , Reactores Biológicos , Digestión , Etanol , Metano
16.
Bioresour Technol ; 330: 124966, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33744734

RESUMEN

The enhancement effects of biochar to an anaerobic membrane bioreactor (AnMBR) treating sewage at low temperatures was investigated in this study through analyzing organics removal, digestion performance, mixed liquor properties, membrane resistance, and foulant compositions. The chemical oxygen demand (COD) removal efficiency and the COD converted to methane rate increased by more than 12.5% at 10 °C, mainly because of the promotion of biochar to volatile fatty acids degradation. Although biochar caused higher dissolved organic matter (DOM) concentration in the AnMBR, it improved the filtration property of the bulk sludge and absorbed the hydrophobic DOM. The decreased filtration resistance assisted by biochar leads to a prolonged membrane operation duration over 200%. Surface foulants, especially cake foulants, were largely mitigated by the enhanced scouring intensity of mixed liquor at the membrane surface, and hence, decreasing the cake/gel foulants ratio.


Asunto(s)
Reactores Biológicos , Aguas del Alcantarillado , Anaerobiosis , Carbón Orgánico , Digestión , Membranas Artificiales , Temperatura , Eliminación de Residuos Líquidos , Aguas Residuales
17.
Bioresour Technol ; 330: 124978, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33770732

RESUMEN

The implementation of anaerobic membrane bioreactor as mainstream technology would reduce the load of sidestream anaerobic digesters. This research evaluated the techno-economic implications of co-digesting sewage sludge and food waste in such wastewater treatment plants to optimise the usage of the sludge line infrastructure. Three organic loading rates (1.0, 1.5 and 2.0 kg VS m-3 d-1) and different strategies to manage the additional nutrients backload were considered. Results showed that the higher electricity revenue from co-digesting food waste offsets the additional costs of food waste acceptance infrastructure and biosolids disposal. However, the higher electricity revenue did not offset the additional costs when the nutrients backload was treated in the sidestream (partial-nitritation/anammox and struvite precipitation). Biosolids disposal was identified as the most important gross cost contributor in all the scenarios. Finally, a sensitivity analysis showed that food waste gate fee had a noticeable influence on co-digestion economic feasibility.


Asunto(s)
Eliminación de Residuos , Purificación del Agua , Anaerobiosis , Reactores Biológicos , Digestión , Alimentos , Metano , Aguas del Alcantarillado , Eliminación de Residuos Líquidos , Aguas Residuales
18.
Bioresour Technol ; 330: 124994, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33773264

RESUMEN

Anaerobic co-digestion of primary sludge with two types of drinking water treatment sludge (DWTS), namely iron- or aluminum-rich DWTS (Fe- or Al-DWTS) were systematically evaluated by biochemical methane potential tests, kinetic modelling, downstream process parameters and microbial community analysis. Specific methane yields decreased approximately 19% to 123 mL·g-1 VS, while the hydrolysis constant kh decreased from 0.21 d-1 to 0.18 d-1 for Fe-DWTS at 10% to 40% dosages. On the contrary, specific methane yields decreased 45-55% for Al-DWTS, and kh decreased to 0.14 d-1 at 40% dosage. Significant removals (>95%) of phosphate and hydrogen sulfide were observed for Fe- and Al-DWTS additions at 40% dosage. Microbial community analysis revealed that Al-DWTS increased the abundance of most hydrogenotrophic methanogens, while Fe-DWTS increased the abundance of acetoclastic methanogens. Kinetic modelling further revealed that Fe- and Al-DWTS additions affected the hydrolysis and methanogenesis process kinetics and the methane yield differently.


Asunto(s)
Agua Potable , Purificación del Agua , Anaerobiosis , Reactores Biológicos , Digestión , Metano , Aguas del Alcantarillado
19.
Bioresour Technol ; 330: 125001, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33773269

RESUMEN

Anaerobic digestion is a long-established technology for the valorization of diverse organic wastes with concomitant generation of valuable resources. However, mono-digestion (i.e., anaerobic digestion using one feedstock) suffers from challenges associated with feedstock characteristics. Co-digestion using multiple feedstocks provides the potential to overcome these limitations. Significant research and development efforts have highlighted several inherent merits of co-digestion, including enhanced digestibility due to synergistic effects of co-substrates, better process stability, and higher nutrient value of the produced co-digestate. However, studies focused on the underlying effects of diverse co-feedstocks on digester performance and stability have not been synthesized so far. This review fills this gap by highlighting the limitations of mono-digestion and critically examining the benefits of co-digestion. Furthermore, this review discusses synergistic effect of co-substrates, characterization of microbial communities, the prediction of biogas production via different kinetic models, and highlights future research directions for the development of a sustainable biorefinery.


Asunto(s)
Reactores Biológicos , Microbiota , Anaerobiosis , Biocombustibles , Digestión , Metano
20.
Bioresour Technol ; 330: 124999, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33780712

RESUMEN

The energy potential of residue-derived biogas via electricity and biomethane production was assessed in an integrated 1G2G sugarcane biorefinery concept. The mono-digestion of 1G-vinasse (1G-VN) was compared with different co-digestion systems, namely, 1G-VN + filter cake (FC) + deacetylation liquor (DL) in the season and FC + DL in the off-season. Gross energy output values and the resulting sugarcane use efficiency were also assessed in different biorefinery schemes. Electricity production from 1G to VN (5.0 MW) could be increased by over 400% through its co-digestion with FC and DL (22.3 MW). Alternatively, biomethane could fully supply the diesel-powered fleet (1.8 × 106 Nm3 month-1) of a sugarcane plant processing 10 million tons of sugarcane per harvest, and the surplus biogas could flexibly provide 36 MW of extra electricity. Biomethane could enhance the energy output of 1G2G sugarcane biorefineries by 15%. However, 2G processes still require marked improvements to maximize energy production from sugarcane.


Asunto(s)
Saccharum , Anaerobiosis , Biocombustibles , Digestión , Electricidad , Metano
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...