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1.
Aquat Toxicol ; 234: 105813, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33812311

RESUMEN

The production of large amounts of synthetic industrial and biomedical compounds, together with environmental pollutants, poses a risk to our ecosystem and induces negative effects on the health of wildlife and human beings. With the emergence of the global problem of chemical contamination, the adverse biological effects of these chemicals are gaining attention among the scientific communities, industry, governments, and the public. Among these chemicals, endocrine disrupting chemicals (EDCs) are regarded as one of the major global issues that potentially affecting our health. There is an urgent need of understanding the potential hazards of such chemicals. Zebrafish have been widely used in the aquatic toxicology. In this review, we first discuss the strategy of transgenic lines that used in the toxicological studies, followed by summarizing the current omics approaches (transcriptomics, proteomics, metabolomics, and epigenomics) on toxicities of EDCs in this model. We will also discuss the possible transgenerational effects in zebrafish and future prospective of the integrated omics approaches with customized transgenic organism. To conclude, we summarize the current findings in the field, and provide our opinions on future environmental toxicity research in the zebrafish model.


Asunto(s)
Epigenómica , Metabolómica , Proteómica , Contaminantes Químicos del Agua/toxicidad , Pez Cebra/metabolismo , Animales , Animales Modificados Genéticamente/metabolismo , Ecosistema , Disruptores Endocrinos/toxicidad , Modelos Animales , Pez Cebra/crecimiento & desarrollo
2.
Artículo en Inglés | MEDLINE | ID: mdl-33801606

RESUMEN

Studying the human health impacts of pesticides and their endocrine disruptor (ED) effects is a public health concern. The aim of this study is to identify phytopharmaceutical active substances (PAS) that are an ED or are toxic on endocrine glands (TEG), and to propose an ED/TEG effect indicator. Five international official databases were analyzed to identify the occurrence of health outcomes for 458 PAS. Health outcomes targeting seven endocrine systems were selected. For each substance, the level of evidence of the collected information and the number of outcomes were used to affect a level of concern about ED/TEG effects. Among the substances studied, 10% had a global ED/TEG effect classified as 'high concern', 55% as 'medium concern', 9% as 'low concern', and 26% as 'unknown'. Ten of the high ED/TEG concern substances and 170 medium or low concern substances were licensed in 2018 in France. The outcomes were mainly on the reproductive organs, thyroid, and adrenal glands. Eight of the 41 biocontrol products studied were classified: 5 were 'high' or 'medium concern' and 3 had 'unknown effect'. Although the proposed ED/TEG indicator is not an official classification, it can be used as an epidemiological tool for classifying the occupational and environmental risks of substances in retrospective population studies and be useful for occupational health physicians.


Asunto(s)
Disruptores Endocrinos , Glándulas Endocrinas , Agricultura , Protección de Cultivos , Disruptores Endocrinos/toxicidad , Sistema Endocrino , Francia , Humanos , Estudios Retrospectivos
3.
Artículo en Inglés | MEDLINE | ID: mdl-33807420

RESUMEN

Endometriosis is an inflammatory and estrogen-dependent gynecological disease associated with exposure to environmental endocrine disruptors. n-Butyl benzyl phthalate (BBP), a ubiquitous plasticizer, has weak estrogenic activity, and exposure to BBP is associated with endometriosis. We aimed to elucidate the immunomodulatory effect of BBP on endometriosis development. We previously established a surgery-induced endometriosis-like murine model. In the present study, we exposed those mice to BBP 10 days prior to surgery and 4 weeks after surgery at physiologically relevant doses to mimic human exposure. Chronic exposure to BBP did not promote the growth of endometriotic lesions; however, the lesion survival rate in BBP-treated mice did increase significantly compared with control mice. Multiparametric flow cytometry showed that BBP exposure did not affect the homeostasis of infiltrated immune subsets in lesions but did enhance CD44 (adhesion marker) expression on plasmacytoid dendritic cells (pDCs). Blocking CD44 interactions locally inhibited endometriotic lesion growth. Immunofluorescence results further confirmed that CD44 blocking inhibited pDC infiltration and reduced the frequency of CD44+ pDCs in endometriotic tissues. BBP also disrupted the estrus cycle in these mice. This study suggests that chronic exposure to low-dose BBP may promote survival of endometriotic tissue through CD44-expressing pDCs.


Asunto(s)
Disruptores Endocrinos , Endometriosis , Ácidos Ftálicos , Animales , Femenino , Humanos , Ratones , Plastificantes/toxicidad
4.
Int J Mol Sci ; 22(6)2021 Mar 11.
Artículo en Inglés | MEDLINE | ID: mdl-33799614

RESUMEN

The estrogen receptors α (ERα) are transcription factors involved in several physiological processes belonging to the nuclear receptors (NRs) protein family. Besides the endogenous ligands, several other chemicals are able to bind to those receptors. Among them are endocrine disrupting chemicals (EDCs) that can trigger toxicological pathways. Many studies have focused on predicting EDCs based on their ability to bind NRs; mainly, estrogen receptors (ER), thyroid hormones receptors (TR), androgen receptors (AR), glucocorticoid receptors (GR), and peroxisome proliferator-activated receptors gamma (PPARγ). In this work, we suggest a pipeline designed for the prediction of ERα binding activity. The flagged compounds can be further explored using experimental techniques to assess their potential to be EDCs. The pipeline is a combination of structure based (docking and pharmacophore models) and ligand based (pharmacophore models) methods. The models have been constructed using the Environmental Protection Agency (EPA) data encompassing a large number of structurally diverse compounds. A validation step was then achieved using two external databases: the NR-DBIND (Nuclear Receptors DataBase Including Negative Data) and the EADB (Estrogenic Activity DataBase). Different combination protocols were explored. Results showed that the combination of models performed better than each model taken individually. The consensus protocol that reached values of 0.81 and 0.54 for sensitivity and specificity, respectively, was the best suited for our toxicological study. Insights and recommendations were drawn to alleviate the screening quality of other projects focusing on ERα binding predictions.


Asunto(s)
Disruptores Endocrinos/química , Receptor alfa de Estrógeno/química , Simulación del Acoplamiento Molecular , Sitios de Unión , Bases de Datos de Compuestos Químicos , Conjuntos de Datos como Asunto , Disruptores Endocrinos/clasificación , Disruptores Endocrinos/metabolismo , Receptor alfa de Estrógeno/metabolismo , Humanos , Ligandos , Unión Proteica , Proyectos de Investigación , Sensibilidad y Especificidad , Relación Estructura-Actividad , Estados Unidos , United States Environmental Protection Agency
5.
Environ Monit Assess ; 193(5): 246, 2021 Apr 06.
Artículo en Inglés | MEDLINE | ID: mdl-33821337

RESUMEN

This study aimed to investigate the occurrence of drugs and endocrine disrupters in water supplies and in water for human consumption. Twelve sampling campaigns were carried out during the rainy and dry season at four sampling points in the Bolonha Complex, in the city of Belém, northern region of Brazil: Bolonha reservoir (catchment) and Water Treatment Plant (WTP) Bolonha (filtered water chamber, treated water tank, and washing water from the filters). The determination of the compounds was performed by solid phase extraction followed by gas and liquid chromatography coupled to mass spectrometry. The results confirmed the anthropic influence that the reservoir and WTP-Bolonha have been suffering, as consequence of the discharge of domestic sewage in natura. Among 25 microcontaminants analyzed, 12 were quantified in raw water and 10 in treated water. The antiallergic Loratadine (LRT) was the contaminant that occurred most frequently in all sample points, having been poorly removed (median 12%) in the conventional treatment used. Losartana (LST), 4-octylphenol (4-OP), and Bisphenol A (BPA) also occurred very frequently in raw water with concentrations ranging from 3.7 to 194 ng L-1. Although such contaminants occurred in treated water in concentrations varying from 4.0 to 135 ng L-1, the estimated margin of exposure ranged from 55 to 3333 times which indicates low risk of human exposure to such contaminants through ingestion of treated water.


Asunto(s)
Disruptores Endocrinos , Preparaciones Farmacéuticas , Contaminantes Químicos del Agua , Purificación del Agua , Brasil , Disruptores Endocrinos/análisis , Monitoreo del Ambiente , Humanos , Contaminantes Químicos del Agua/análisis
6.
J Environ Pathol Toxicol Oncol ; 40(2): 65-79, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33822518

RESUMEN

Environmental pollution (EP) is a well-known threat to wild animals, but its toxicological impact is poorly understood. In vitro toxicity evaluation using cells of lower predators could be a promising way to assess and monitor the effects of EPs on whole wildlife populations that are related in the food web. Here, we describe EPs' toxic effect and mechanism in the primary fibroblast derived from the embryo of the striped field mouse, Apodemus agrarius. Characterization of the primary fibroblast was via morphology, genetics, immunocytochemistry, and stable culture conditions for optimal toxicity screening. Cell viability assays-MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and lactate dehydrogenase (LDH)-were performed to observe cytotoxicity, and quantitative PCR was conducted to confirm gene alteration by EP exposure. MTT and LDH assays confirmed the cytotoxicity of transfluthrin (TF), benzyl butyl phthalate (BBP), and 17ß-estradiol (E2) with IC50 values of 10.56 µM, 10.82 µM, and 24.08 µM, respectively, following 48-h exposures. mRNA expression of androgen-binding protein, growth hormone receptor, cytochrome C oxidase, and cytochrome P450-1A1 was induced after exposure to TF, BBP, and E2. We unveiled new EP mechanisms at the mammalian cellular level and discovered potential biomarker genes for monitoring of EPs. Based on our findings, we propose the primary fibroblast of A. agrarius as a valuable model to assess the toxicological effects of EP on wildlife.


Asunto(s)
Ciclopropanos/toxicidad , Disruptores Endocrinos/toxicidad , Estradiol/toxicidad , Estrógenos/toxicidad , Fibroblastos/efectos de los fármacos , Fluorobencenos/toxicidad , Insecticidas/toxicidad , Ácidos Ftálicos/toxicidad , Proteína de Unión a Andrógenos/genética , Animales , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Ciclooxigenasa 1/genética , Citocromo P-450 CYP1A1/genética , Embrión de Mamíferos/citología , Fibroblastos/metabolismo , Murinae , Receptores de Somatotropina/genética
7.
Int J Mol Sci ; 22(6)2021 Mar 19.
Artículo en Inglés | MEDLINE | ID: mdl-33808818

RESUMEN

Dichlorodiphenyltrichloroethane (DDT) is the most widespread, persistent pollutant and endocrine disruptor on the planet. Although DDT has been found to block androgen receptors, the effects of its low-dose exposure in different periods of ontogeny on the male reproductive system remain unclear. We evaluate sex steroid hormone production in the pubertal period and after maturation in male Wistar rats exposed to low doses of o,p'-DDT, either during prenatal and postnatal development or postnatal development alone. Prenatally and postnatally exposed rats exhibit lower testosterone production and increased estradiol and estriol serum levels after maturation, associated with the delayed growth of gonads. Postnatally exposed rats demonstrate accelerated growth of gonads and higher testosterone production in the pubertal period. In contrast to the previous group, they do not present raised estradiol production. All of the exposed animals exhibit a reduced conversion of progesterone to 17OH-progesterone after sexual maturation, which indicates putative attenuation of sex steroid production. Thus, the study reveals age-dependent outcomes of low-dose exposure to DDT. Prenatal onset of exposure results in the later onset of androgen production and the enhanced conversion of androgens to estrogens after puberty, while postnatal exposure induces the earlier onset of androgen secretion.


Asunto(s)
Andrógenos/biosíntesis , DDT/farmacología , Disruptores Endocrinos/farmacología , Exposición a Riesgos Ambientales/efectos adversos , Estrógenos/biosíntesis , Animales , DDT/administración & dosificación , Disruptores Endocrinos/administración & dosificación , Femenino , Genitales Masculinos/efectos de los fármacos , Genitales Masculinos/metabolismo , Hormonas Esteroides Gonadales/biosíntesis , Gónadas/efectos de los fármacos , Gónadas/metabolismo , Masculino , Ratas
8.
Environ Sci Pollut Res Int ; 28(16): 19643-19663, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33666848

RESUMEN

Bisphenol A [BPA; (CH3)2C(C6H4OH)2] is a synthetic chemical used as a precursor material for the manufacturing of plastics and resins. It gained attention due to its high chances of human exposure and predisposing individuals at extremely low doses to diseases, including cancer. It enters the human body via oral, inhaled, and dermal routes as leach-out products. BPA may be anticipated as a probable human carcinogen. Studies using in vitro cell lines, rodent models, and epidemiological analysis have convincingly shown the increasing susceptibility to cancer at doses below the oral reference dose set by the Environmental Protection Agency for BPA. Furthermore, BPA exerts its toxicological effects at the genetic and epigenetic levels, influencing various cell signaling pathways. The present review summarizes the available data on BPA and its potential impact on cancer and its clinical outcome.


Asunto(s)
Disruptores Endocrinos , Compuestos de Bencidrilo/toxicidad , Carcinógenos/toxicidad , Fenoles/toxicidad
9.
Ecotoxicology ; 30(4): 658-666, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33730294

RESUMEN

A new class of biosurfactants, namely quaternary ammonium sophorolipids (SQAS), suitable for pharmaceutical applications, was tested for the evaluation of their (anti)estrogenic and (anti)androgenic potency with the help of YES/YAS assays. Also their toxicity towards yeasts (Saccharomyces cerevisiae) and bacteria (Escherichia coli) was checked. The results achieved for SQAS, which can be regarded as potential micropollutants, were compared with those obtained for two well-known micropollutants diclofenac and 17α-ethinylestradiol subjected to the same testing procedures. This work demonstrated that acetylation of the hydroxyl group of the carbohydrate head of SQAS decreased the toxicity of this class of biosurfactants towards Saccharomyces cerevisiae. Furthermore, it contributed to the decrease of their endocrine disrupting potency. None of the SQAS studied showed clear agonist activity for female or male hormones. SQAS1 and SQAS2 revealed weak antiestrogenic and antiandrogenic potency. All of these properties were weaker, not only to the potency of the appropriate positive control in the antagonists bioassays, but also compared to the potency of other tested compounds, i.e. DCF and EE2. SQAS3 possessed most probably inhibitory activity towards male hormones. Moreover, cytotoxicity of two out of four studied SQAS at the highest concentrations towards the strains of Saccharomyces cerevisiae interfered with the endocrine disruption activity. It would be also worth studying it with the use of another endocrine activity test.


Asunto(s)
Compuestos de Amonio , Disruptores Endocrinos , Disruptores Endocrinos/toxicidad , Etinilestradiol , Femenino , Masculino , Ácidos Oléicos , Sales (Química)
10.
Ecotoxicol Environ Saf ; 215: 112136, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-33735605

RESUMEN

The combined toxicological assessment provides a realistic approach for hazard evaluation of chemical cocktails that co-existed in the environment. This review provides a holistic insight into the studies highlighting the mixture toxicity of the endocrine-disrupting chemicals (EDCs), especially focusing on the screening of biochemical pathways and other toxicogenetic endpoints. Reviewed literature showed that numerous multiplexed toxicogenomic techniques were applied to determine reproductive effects in vertebrates, but limited studies were found in non-mammalian species after mixture chemical exposure. Further, we found that the experimental design and concentration selection are the two important parameters in mixture toxicity studies that should be time- and cost-effective, highly precise, and environmentally relevant. A summary of EDC mixtures affecting the thyroid axis, estrogen axis, androgen axis, growth stress, and immune system via in vivo bioassays was also presented. It is interesting to mention that majority of estrogenic effects of the mixtures were sex-dependent, particularly observed in male fish as compared to female fish. Further, the androgen axis was perturbed with serious malformations in male rat testis (epididymal or gubernacular lesions, and deciduous spermatids). Also, transgenerational epigenetic effects were promoted in the F3 and F4 generations in the form of DNA methylation epimutations in sperm, increasing polycystic ovaries and reducing the offspring. Similarly, increased oxidative stress, high antioxidant enzymatic activities, disturbed estrous cycle, and decreased steroidogenesis were the commonly found effects after acute or chronic exposure to EDC mixtures. Importantly, the concentration addition (CA) and independent action (IA) models became more prevalent and suitable predictive models to unveil the prominence of synergistic estrogenic and anti-androgenic effects of chemical mixtures. More importantly, this review encompasses the research challenges and gaps in the existing knowledge and specific future research perspectives on combined toxicity.


Asunto(s)
Disruptores Endocrinos/toxicidad , Antagonistas de Andrógenos , Animales , Bioensayo , Metilación de ADN , Estrógenos/toxicidad , Femenino , Peces , Masculino , Ratas , Reproducción , Espermatozoides , Glándula Tiroides
11.
Environ Pollut ; 278: 116853, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-33740605

RESUMEN

In the last few decades, Endocrine Disrupting Chemicals (EDCs) have taken significant roles in creating harmful effects to aquatic organisms. Many proposed treatment applications are time consuming, expensive and focus mainly on waste water treatment plants (WWTP), which are indeed a major aquatic polluting source. Nonetheless, the marine environment is the ultimate sink of many pollutants, e.g. EDCs, and has been largely neglected mainly due to the challenge in treating such salty and immense open natural ecosystems. In this study we describe the bromination and the yet unpresented degradation process of high concentrations (5 mg/L) of phenolic EDCs, by the marine red macroalgaeGracilaria sp. As shown, 17α-Ethinylestradiol (EE2), a well-known contraceptive drug, and one of the most persistent phenol EDCs in the environment, was eliminated from both the medium and tissues of the macroalga, in addition to the degradation of all metabolites as verified by the nil estrogenic activity recorded in the medium. Validation of the proposed bromination-degradation route was reinforced by identifying Bisphenol A (BPA) brominated degradation products only, following 168H of incubation in the presence of Gracilaria sp. As demonstrated in this assay for EE2, BPA and finally for paracetamol, it is likely that the phenol scavenging activity is nonspecific and, thus, possibly even a wider scope of various other phenol-based pollutants might be treated in coastal waters. As far as we know, Gracilaria sp. is the only marine sessile organism able of degrading various phenol based pollutants. The worldwide distribution of many Gracilaria species and their wide aquaculture knowhow, suggest that bioremediation based on these seaweeds is a possible cost effective progressive solution to the treatment of a wide scope of phenols at the marine environment.


Asunto(s)
Disruptores Endocrinos , Contaminantes Ambientales , Gracilaria , Algas Marinas , Contaminantes Químicos del Agua , Compuestos de Bencidrilo , Biodegradación Ambiental , Ecosistema , Disruptores Endocrinos/análisis , Fenol , Fenoles/análisis , Contaminantes Químicos del Agua/análisis
12.
Environ Sci Technol ; 55(6): 3736-3746, 2021 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-33650859

RESUMEN

A wide range of pharmaceuticals and endocrine disrupting compounds enter freshwaters globally. As these contaminants are transported through aquatic food webs, understanding their impacts on both aquatic and terrestrial ecosystems remains a major challenge. Here, we provide the first direct evidence of the transfer of pharmaceuticals and endocrine disruptors through the aquatic-terrestrial habitat linkage by emerging aquatic insects. We also show that the type of insect metamorphosis and feeding behavior determine the bioaccumulation patterns of these contaminants. Adult Trichoptera, an important food source for riparian predators, showed an increased body burden of pharmaceuticals and endocrine disruptors. This implies that terrestrial predators, such as spiders, birds, and bats, are exposed to mixtures of pharmaceuticals and endocrine disruptors of aquatic origin, which may impact their physiology and population dynamics. Overall, our study provides valuable insights into the bioaccumulation patterns and trophic cross-ecosystem transfer of these contaminants, from aquatic primary producers to terrestrial predators.


Asunto(s)
Disruptores Endocrinos , Preparaciones Farmacéuticas , Animales , Ecosistema , Cadena Alimentaria , Insectos
13.
Artículo en Inglés | MEDLINE | ID: mdl-33672279

RESUMEN

Background: Differential exposure to endocrine-disrupting chemicals, including phthalate diesters, may contribute to persistent racial/ethnic disparities in women's reproductive health outcomes. We sought to characterize sources of gestational exposure to these agents that may differ according to maternal race. Methods: We enrolled pregnant Black (n = 198), including African American, and White (n = 197) women during the second trimester, and measured eight phthalate monoester metabolites in urine. We assessed confounder-adjusted associations between multiple food and beverage consumption habits, summarized using a principal component analysis, as predictors of maternal urinary phthalate metabolite levels, stratified by race. Results: Whites reported significantly greater unprocessed food consumption (42.5% vs. 32.0%; p < 0.001) and storage of food in clear unbreakable plastic containers (66.5% vs. 49.3%; p < 0.001) than Blacks, while Blacks consumed more canned fruits and vegetables (23.5% vs. 12.2%; p < 0.001) than Whites. Using plastics for food storage, microwaving in plastic containers, and using hard plastic water bottles was associated with urinary phthalate concentrations, especially DEHP metabolites (e.g., mean difference = 5.13%; 95% CI: 3.05, 7.25). These associations were driven primarily by Black pregnant women. Conclusions: Targeted interventions to reduce maternal exposure to phthalates need to be designed with specific attention to differences in food and beverage consumption behaviors among Black and White women.


Asunto(s)
Disruptores Endocrinos , Contaminantes Ambientales , Ácidos Ftálicos , Bebidas , Exposición a Riesgos Ambientales/análisis , Femenino , Humanos , Exposición Materna , Ácidos Ftálicos/análisis , Embarazo
14.
Int J Mol Sci ; 22(4)2021 Feb 21.
Artículo en Inglés | MEDLINE | ID: mdl-33670069

RESUMEN

Endocrine disruptors are a group of chemical compounds that, even in low concentrations, cause a hormonal imbalance in the body, contributing to the development of various harmful health disorders. Many industry compounds, due to their important commercial value and numerous applications, are produced on a global scale, while the mechanism of their endocrine action has not been fully understood. In recent years, per- and polyfluoroalkyl substances (PFASs) have gained the interest of major international health organizations, and thus more and more studies have been aimed to explain the toxicity of these compounds. PFASs were firstly synthesized in the 1950s and broadly used in the industry in the production of firefighting agents, cosmetics and herbicides. The numerous industrial applications of PFASs, combined with the exceptionally long half-life of these substances in the human body and extreme environmental persistence, result in a common and chronic exposure of the general population to their action. Available data have suggested that human exposure to PFASs can occur during different stages of development and may cause short- or/and long-term health effects. This paper synthetizes the current literature reports on the presence, bioaccumulation and, particularly, endocrine toxicity of selected long- and short-chain PFASs, with a special emphasis on the mechanisms underlying their endocrine actions.


Asunto(s)
Disruptores Endocrinos/toxicidad , Fluorocarburos/toxicidad , Animales , Biotransformación/efectos de los fármacos , Fenómenos Químicos , Disruptores Endocrinos/química , Fluorocarburos/sangre , Fluorocarburos/química , Fluorocarburos/orina , Humanos , Modelos Biológicos
17.
Ecotoxicol Environ Saf ; 214: 112114, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33711575

RESUMEN

Endocrine disrupting chemicals can mimic, block, or interfere with hormones in organisms and subsequently affect their development and reproduction, which has raised significant public concern over the past several decades. To investigate (quantitative) structure-activity relationship, 8280 compounds were compiled from the Tox21 10K compound library. The results show that 50% activity concentrations of agonists are poorly related to that of antagonists because many compounds have considerably different activity concentrations between the agonists and antagonists. Analysis on the chemical classes based on mode of action (MOA) reveals that estrogen receptor (ER) is not the main target site in the acute toxicity to aquatic organisms. Binomial analysis of active and inactive ER agonists/antagonists reveals that ER activity of compounds is dominated by octanol/water partition coefficient and excess molar refraction. The binomial equation developed from the two descriptors can classify well active and inactive ER chemicals with an overall prediction accuracy of 73%. The classification equation developed from the molecular descriptors indicates that estrogens react with the receptor through hydrophobic and π-n electron interactions. At the same time, molecular ionization, polarity, and hydrogen bonding ability can also affect the chemical ER activity. A decision tree developed from chemical structures and their applications reveals that many hormones, proton pump inhibitors, PAHs, progestin, insecticides, fungicides, steroid and chemotherapy medications are active ER agonists/antagonists. On the other hand, many monocyclic/nonaromatic chain compounds and herbicides are inactive ER compounds. The decision tree and binomial equation developed here are valuable tools to predict active and inactive ER compounds.


Asunto(s)
Disruptores Endocrinos/clasificación , Antagonistas de Estrógenos/clasificación , Estrógenos/clasificación , Receptores Estrogénicos/antagonistas & inhibidores , Árboles de Decisión , Disruptores Endocrinos/química , Disruptores Endocrinos/farmacología , Antagonistas de Estrógenos/química , Antagonistas de Estrógenos/farmacología , Estrógenos/química , Estrógenos/farmacología , Relación Estructura-Actividad Cuantitativa , Bibliotecas de Moléculas Pequeñas
18.
Mar Pollut Bull ; 166: 112241, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33711611

RESUMEN

The occurrence of endocrine disrupting chemicals (EDCs) has been determined in two widely consumed fish species from Persian Gulf i.e., Epinephelus coioides and Platycephalus indicus by applying a validated analytical for the simultaneous detection of fourteen EDCs. The concentrations of all detected EDCs were greater in the liver than in the muscle (except for bisphenol A in P. indicus), suggesting a prolonged exposure of the fishes to these pollutants in the Persian Gulf. Specifically, the results showed that di (2-ethylhexyl) phthalate (DEHP) was the compound detected most frequently and at the highest concentration in both species. DEHP levels in ranged from 6.68 to 297.48 µg g-dw-1 and from 13.32 to 350.52 µg g-dw-1, in muscle and in liver, respectively. A risk assessment study was conducted, and demonstrated that consuming two fish based- meals per week may result in a moderate risk especially for vulnerable population groups.


Asunto(s)
Disruptores Endocrinos , Contaminantes Químicos del Agua , Animales , Disruptores Endocrinos/análisis , Monitoreo del Ambiente , Humanos , Océano Índico , Medición de Riesgo , Contaminantes Químicos del Agua/análisis
19.
Sci Total Environ ; 768: 144750, 2021 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-33736315

RESUMEN

Recent urban public water supply contamination events emphasize the importance of screening treated drinking water quality after distribution. In vitro bioassays, when run concurrently with analytical chemistry methods, are effective tools to evaluating the efficacy of water treatment processes and water quality. We tested 49 water samples representing the Chicago Department of Water Management service areas for estrogen, (anti)androgen, glucocorticoid receptor-activating contaminants and cytotoxicity. We present a tiered screening approach suitable to samples with anticipated low-level activity and initially tested all extracts for statistically identifiable endocrine activity; performing a secondary dilution-response analysis to determine sample EC50 and biological equivalency values (BioEq). Estrogenic activity was detected in untreated Lake Michigan intake water samples using mammalian (5/49; median: 0.21 ng E2Eq/L) and yeast cell (5/49; 1.78 ng E2Eq/L) bioassays. A highly sensitive (anti)androgenic activity bioassay was applied for the first time to water quality screening and androgenic activity was detected in untreated intake and treated pre-distribution samples (4/49; 0.93 ng DHTEq/L). No activity was identified above method detection limits in the yeast androgenic, mammalian anti-androgenic, and both glucocorticoid bioassays. Known estrogen receptor agonists were detected using HPLC/MS-MS (estrone: 0.72-1.4 ng/L; 17α-estradiol: 1.3-1.5 ng/L; 17ß-estradiol: 1.4 ng/L; equol: 8.8 ng/L), however occurrence did not correlate with estrogenic bioassay results. Many studies have applied bioassays to water quality monitoring using only relatively small samples sets often collected from surface and/or wastewater effluent. However, to realistically adapt these tools to treated water quality monitoring, water quality managers must have the capacity to screen potentially hundreds of samples in short timeframes. Therefore, we provided a tiered screening model that increased sample screening speed, without sacrificing statistical stringency, and detected estrogenic and androgenic activity only in pre-distribution Chicago area samples.


Asunto(s)
Disruptores Endocrinos , Contaminantes Químicos del Agua , Animales , Bioensayo , Chicago , Disruptores Endocrinos/análisis , Monitoreo del Ambiente , Estrógenos/análisis , Michigan , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad , Calidad del Agua
20.
Arch Environ Contam Toxicol ; 80(4): 726-734, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33774695

RESUMEN

The thyroid hormones, thyroxine (T4) and triiodothyronine (T3), are required to regulate complex developmental processes in vertebrates and are highly sensitive to endocrine-disrupting compounds. Previous studies demonstrate that dioctyl sodium sulfosuccinate (DOSS), a common constituent of pharmaceuticals, cosmetics, and food products, disrupts canonical signaling of adipocyte differentiation by binding a nuclear hormone receptor in the same superfamily as thyroid hormone (TH) receptors. The present study was designed to determine whether DOSS is capable of disrupting TH signaling using the American bullfrog, Rana (Lithobates) catesbeiana-a cosmopolitan frog species that undergoes TH-dependent metamorphosis to transition from an aquatic tadpole to a terrestrial juvenile frog. Premetamorphic R. catesbeiana tadpoles were injected with 2 pmol/g body weight T3 or 10 pmol/g body weight T4 to induce precocious metamorphosis, then exposed for 48 h to environmentally or clinically relevant DOSS concentrations (0.5, 5, and 50 mg/L). Gene expression of three classical TH-responsive targets (thra, thrb, and thibz) was measured in tadpole liver and tail fin tissue through reverse transcription quantitative polymerase chain reaction (RT-qPCR). DOSS disrupted gene expression in liver and tail fin tissue at all three concentrations tested but the patterns of expression differed by tissue, gene transcript, and TH treatment status. To our knowledge, this is the first demonstration that DOSS can alter TH signaling. Further exploration into DOSS disruption of TH signaling is warranted, because exposure may affect other TH-dependent processes, such as salmon smoltification and perinatal human development.


Asunto(s)
Disruptores Endocrinos , Animales , Disruptores Endocrinos/toxicidad , Humanos , Larva , Metamorfosis Biológica , Rana catesbeiana , Ranidae , Sodio , Succinatos , Hormonas Tiroideas , Estados Unidos
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