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Gene ; 766: 145117, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32920039


The extracellular vesicles (EVs) of uterine flushing fluids (UFs) mediate intrauterine communication between conceptus and uterus in pigs. The small RNAs of UFs-EVs are widely recognized as important factors that influence embryonic implantation. However, small RNAs expression profiles of porcine UFs-EVs during peri-implantation are still unknown. In this study, cup-shaped EVs of porcine UFs on days 10 (D10), 13 (D13) and 18 (D18) of pregnancy were isolated and characterized. The expression of small RNAs in these EVs was comprehensively profiled through sequencing. A total of 152 known microRNAs (miRNAs), 43 novel miRNAs, 6248 known Piwi-interacting RNAs (piRNAs) and 110 novel piRNAs were identified. Among these small RNAs, RT-qRCR results indicated that ssc-let-7f-5p, ssc-let-7i-5p and ssc-let-7g were differentially expressed during the three stages. Bioinformatics analysis showed that the miRNAs differentially expressed in the three comparisons (D10 vs D13, D13 vs D18 and D10 vs D18) were involved in important processes and pathways related to immunization, endometrial receptivity and embryo development, which play important roles in embryonic implantation. Our results reveal that EVs from porcine UFs contain various small RNAs with potentially vital effects on implantation. This research also provides resources for studies of miRNAs and piRNAs in the cross-talk between embryo and endometrium.

Implantación del Embrión/genética , Vesículas Extracelulares/genética , MicroARNs/genética , Útero/fisiología , Animales , Desarrollo Embrionario/genética , Endometrio/fisiología , Femenino , Embarazo , ARN Interferente Pequeño/genética , RNA-Seq/métodos , Porcinos
Sci Total Environ ; 751: 142235, 2021 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-33181987


Inhaled particulate matter (PM) from combustion- and friction-sourced air pollution adversely affects organs distant from the lung. A putative mechanism for the remote effect of inhaled PM is that ultrafine, nano-sized fraction (<100 nm) translocates across the air-tissue barrier, directly interacting with phagocytic tissue cells. Although PM is reported in other tissues, whether it is phagocytosed by non-respiratory tissue resident cells is unclear. Using the placenta as an accessible organ for phagocytic cells, we sought to seek evidence for air pollution-derived PM in tissue resident phagocytes. Macrophage-enriched placental cells (MEPCs) were isolated, and examined by light and electron microscopy. MEPC carbon was assessed by image analysis (mean µm2/1000 cells); particle composition and numbers were investigated using magnetic analyses and energy dispersive X-ray spectroscopy. MEPCs phagocytic capacity was assessed by culture with diesel exhaust PM in vitro. Fifteen placentas were analysed. Black inclusions morphologically compatible with inhaled PM were identified within MEPCs from all samples (mean ± SEM carbon loading, 1000 MEPCs/participant of 0.004 ± 0.001 µm2). High resolution scanning/transmission electron microscopy revealed abundant nano-sized particle aggregates within MEPCs. MEPC PM was predominantly carbonaceous but also co-associated with a range of trace metals, indicative of high temperature (i.e. exogenous) generation. MEPCs contained readily-measurable amounts of iron-rich, ferrimagnetic particles, in concentrations/particle number concentrations ranging, respectively, from 8 to 50 ng/g and 10 to 60.107 magnetic particles/g (wet wt) MEPCs. Extracted MEPCs (n = 20/ placenta) were phagocytic for PM since all cells showed increased carbon area after culture with diesel PM in vitro (mean ± SEM increase 7.55 ± 1.26 µm2 carbon PM). These findings demonstrate that inhaled, metal-bearing, air pollution-derived PM can not only translocate to distant organs, but is taken up by tissue resident phagocytes in vivo. The human placenta, and hence probably the fetus, thus appears to be a target for such particles.

Contaminantes Atmosféricos , Contaminación del Aire , Nanopartículas , Contaminantes Atmosféricos/análisis , Contaminantes Atmosféricos/toxicidad , Contaminación del Aire/análisis , Femenino , Humanos , Tamaño de la Partícula , Material Particulado/análisis , Embarazo , Emisiones de Vehículos/análisis , Emisiones de Vehículos/toxicidad
Chemosphere ; 262: 127880, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-32777607


BACKGROUND: Bisphenol A (BPA) is a well-known endocrine disruptor that affects male fertility. However, the main biological events through which BPA affects spermatogenesis remain to be identified. METHODS: Adult male mice were treated by feeding with drinking water containing BPA (0.2 µg/ml, 20 µg/ml, 200 µg/ml, respectively) for two months. Testes were collected for protein extraction or for immunohistochemical analysis. Epididymal spermatozoa were collected for sperm quality evaluation and male fertility assay by in vitro fertility (IVF). Serums were collected for detection of testosterone levels. Proteins associated with germ cell proliferation, meiosis, blood-testis barrier, and steroidogenesis production were examined in BPA-treated and control mice testes. CCK8 assay was used to detect the effect of BPA on the proliferation of GC-1 and GC-2 cells. RESULTS: The BPA-treated mice were characterized by decreased sperm quality, serum testosterone levels and, sub-fertile phenotype characterizing with low pregnancy rates and reduced fertilization efficiency. In lower BPA (0.2 µg/ml) treatment, PCNA and PLZF were down-expressed that indicated impaired germ cell proliferation. SYCP3 was down-expressed in BPA-treated mice, but expressions of other proteins associated with meiosis and blood-testis barrier were not significantly altered. CYP11A1 and HSD3B1 were down-expressed in BPA-treated mice that demonstrated reduced steroidogenesis activity. BPA has a concentration-dependent inhibition effect on the proliferation of GC-1 and GC-2 cells. Conclusively, low doses BPA exposure reduced mice sperm quality mainly by impairing germ cell proliferation, leading to reduced male fertility. The study would provide relevant information for investigation on molecular mechanisms and protective strategy on male production.

Compuestos de Bencidrilo/toxicidad , Disruptores Endocrinos/toxicidad , Fenoles/toxicidad , Espermatozoides/fisiología , Animales , Proliferación Celular/efectos de los fármacos , Disruptores Endocrinos/metabolismo , Epidídimo/efectos de los fármacos , Femenino , Fertilidad/efectos de los fármacos , Masculino , Ratones , Embarazo , Espermatogénesis/efectos de los fármacos , Espermatozoides/efectos de los fármacos , Testículo/efectos de los fármacos , Pruebas de Toxicidad Crónica
Sci Total Environ ; 750: 141579, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32853937


BACKGROUND: Understanding the effects of environmental factors on birth outcomes is crucial for public health because newborns' birth size affects their likelihood of childhood survival, risk of perinatal morbidity, and subsequent health and growth. Therefore, we investigated the associations of birth outcomes with prenatal air pollutant exposure and residential land use characteristics in the Greater Taipei Area. METHODS: Participants were selected from the Longitudinal Examination across Prenatal and Postpartum Health in Taiwan study, which is an ongoing prospective study launched in July 2011. Parental sociodemographic data and medical histories were collected using standardized questionnaires. Mean air pollutant levels during each trimester were estimated using the spatial interpolation technique (Ordinary Kriging). Land use types surrounding participants' homes were evaluated within a designated radius of their residential addresses. We used multiple regressions to examine relationships between birth outcomes (i.e., birth weight, height, and head circumference) and environmental factors after adjustment for parental characteristics. RESULTS: A total of 436 pregnant women-infant pairs were included. Birth weight was negatively associated with commercial land and greenhouse areas near the residence. Living near greenhouse areas negatively affected birth height, but higher greenness level within 100 m of the residence had a positive effect. Birth head circumference was only associated with sociodemographic factors in the multivariate model. CONCLUSION: Land use types near the homes of pregnant women, but not exposure to air pollutants, were significantly associated with birth weight and height in the Greater Taipei Area. Increased greenness level was positively associated with birth height, and living near commercial or greenhouse areas had adverse effects on birth outcomes. Living in a healthy neighborhood is critical for the birth outcomes of infants and presumably their health in early childhood.

Contaminantes Atmosféricos , Contaminación del Aire , Contaminantes Atmosféricos/análisis , Contaminación del Aire/análisis , Peso al Nacer , Niño , Preescolar , Femenino , Humanos , Recién Nacido , Embarazo , Estudios Prospectivos , Taiwán/epidemiología
Sci Total Environ ; 751: 141476, 2021 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-32871309


BACKGROUND: More women in reproductive ages are entering occupations where exposure to whole body vibrations (WBV) is common (e.g. in transportation and construction). Previous studies based on self-assessed exposure suggest increased risks of adverse birth outcomes, but it is unclear at what exposure levels and if the current exposure guidelines are appropriate during pregnancy. OBJECTIVES: To investigate whether occupational WBV-exposure increases the risk of preterm birth, low birth weight, and/or small-for-gestational age, in a large, nationwide, prospective, cohort study. DESIGN: The Fetal Air Pollution Exposure cohort (FAIR) was formed by merging data from multiple, national registers, and the present study includes singletons born 1994-2014 to working women in Sweden (n = 1,091,080 births). WBV-exposure was assessed quantitatively using a job-exposure matrix based on measurements, and calculated odds ratios were adjusted for potential confounders such as smoking and BMI, and other occupational exposures like noise, combustion particles, and physically and psychologically strenuous work. Data on absence from work (full-/part time, sick leave, parental leave, etc.) was also used. RESULTS: Exposure to WBV during pregnancy, among women with low absence from work (n = 476,419), was associated with an increased risk of preterm birth, below the occupational exposure limit (1.15 m/s2). Compared to unexposed mothers, the OR was 1.38 (95% CI: 1.05, 1.83) for exposure ≥0.5 m/s2, corresponding to an increase from 47/1000 cases to 65/1000 cases. No increased risk was found for small-for-gestational age. CONCLUSIONS: Exposure to WBV was associated with an increased risk of preterm birth. The results suggest that the current permissible exposure and action levels for WBV-exposure do not adequately protect pregnant women with continuous exposure.

Exposición Profesional , Nacimiento Prematuro , Estudios de Cohortes , Femenino , Humanos , Recién Nacido , Recién Nacido Pequeño para la Edad Gestacional , Exposición Profesional/efectos adversos , Embarazo , Resultado del Embarazo/epidemiología , Nacimiento Prematuro/epidemiología , Estudios Prospectivos , Suecia/epidemiología , Vibración/efectos adversos
Chemosphere ; 262: 128369, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33182099


Due to the mounting evidence that phthalates, specifically di-2-ethylhexyl phthalate and dibutyl phthalate, produce adverse endocrine effects in humans and wildlife, the use of other chemicals as replacements has increased. One of the most commonly encountered phthalate replacements is di(isononyl)cyclohexane-1,2-dicarboxylate (DINCH). Currently, little is known about the prevalence of human exposure, bioactivity, and endocrine disrupting potential of DINCH. We sampled urine from 100 pregnant women during the second trimester of pregnancy living in Charleston, SC between 2011 and 2014 and measured the following DINCH metabolites by LC-MS/MS: cyclohexane-1,2-dicarboxylic acid-mono(hydroxy-isononyl) ester (OH-MINCH), cyclohexane-1,2-dicarboxylic acid-mono(oxo-isononyl) ester (oxo-MINCH), and cyclohexane-1,2-dicarboxylic acid-monocarboxy isooctyl ester (cx-MINCH). These metabolites were also tested on human estrogen receptor alpha and progesterone receptor beta transactivation assays in vitro. OH-MINCH was detected in 98% of urine samples. The specific gravity-adjusted median (interquartile range) OH-MINCH concentration was 0.20 (0.25) ng/mL, and concentrations were significantly higher in African American women compared to Caucasian women (p = 0.01). DINCH metabolite concentrations were consistent between years, and they did not exhibit estrogenic or progestogenic activity in vitro. Human exposure to these emerging compounds should continue to be monitored, especially in vulnerable populations, to ensure the replacement of phthalates by DINCH is not a case of regrettable substitution.

Exposición Materna/estadística & datos numéricos , Adulto , Monitoreo Biológico , Cromatografía Liquida , Ácidos Ciclohexanocarboxílicos/metabolismo , Ciclohexanos , Dibutil Ftalato , Ácidos Dicarboxílicos/metabolismo , Dietilhexil Ftalato , Disruptores Endocrinos , Exposición a Riesgos Ambientales/análisis , Ésteres , Femenino , Humanos , Ácidos Ftálicos , Plastificantes/análisis , Embarazo , South Carolina , Espectrometría de Masas en Tándem
Chemosphere ; 262: 128404, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33182127


BACKGROUND: Reduced growth velocity before birth increases the risk of adverse health outcomes in adult life. However, until recently, there has been a lack of studies demonstrating the impact of prenatal PM2.5 exposure on fetal growth velocity. METHODS: The current study was embedded in a previous cohort built between January 1, 2014, and April 30, 2015, in Shanghai First Maternity and Infant Hospital, China, in 6129 eligible singleton pregnancies. The PM2.5 concentration was estimated by an inverse distance weighted method according to the residential addresses of the participants. Repeated fetal biometry measurements, including head circumference (HC), abdominal circumference (AC), femur length (FL), and biparietal diameter (BPD), were measured through ultrasound between 14 and 41 gestational weeks. A principal component analysis through conditional expectation for sparse longitudinal data was used to estimate the corresponding velocities. RESULTS: A total of 22782 ultrasound measurements were conducted among 6129 participants with a median of 2 and a maximum of 9 measurements. With each 10 µg/m3 increase in cumulative PM2.5 exposure, the velocity of HC, AC FL and BPD decreased by 0.12 mm/week, 0.17 mm/week, 0.02 mm/week and 0.02 mm/week, respectively, on average. The results of the Generalized Functional Concurrent Model showed that the velocity decreased significantly with PM2.5 exposure between 22 and 32 gestational weeks, which might be the potential sensitive exposure window. CONCLUSIONS: There are negative associations between prenatal exposure to PM2.5 and fetal growth velocity, and the late second trimester and early third trimester might be the potential sensitive window.

Contaminantes Atmosféricos/toxicidad , Exposición Materna , Material Particulado/toxicidad , Efectos Tardíos de la Exposición Prenatal , Adulto , China , Estudios de Cohortes , Femenino , Desarrollo Fetal , Edad Gestacional , Humanos , Masculino , Material Particulado/análisis , Embarazo , Segundo Trimestre del Embarazo , Tercer Trimestre del Embarazo , Ultrasonografía Prenatal/métodos
Sci Total Environ ; 752: 141784, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32889265


Emerging evidence suggests that perinatal dioxin exposure affects neurodevelopment and impairs multiple brain functions, including cognitive, language, learning and emotion, in the offspring. However, the impacts of gestational and lactational exposure to dioxin on behavior and related molecular events are still not fully understood. In this study, female C57BL/6J mice were orally administered three doses of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) (0.1 or 10 µg/kg body weight (bw)) during the pregnancy and lactation periods. The locomotion, exploration and anxiety-related behaviors were examined by an open field test of the young adult female offspring at postnatal day 68. We found that the maternal TCDD exposure, particularly at a low dose, increased movement ability, novelty-exploration and certain anxiety-related behaviors in the offspring. Such hyperactivity-like behaviors were accompanied by the upregulation of certain genes associated with cholinergic neurotransmission or synaptogenesis in the offspring brain. In accordance with the potential enhancement of cholinergic neurotransmission due to the gene upregulations, the enzymatic activity of acetylcholinesterase was decreased, which might lead to excess acetylcholine and consequent hyper-excitation at the synapses. Thus, we found that gestational and lactational TCDD exposure at low dose caused hyperactivity-like behaviors in young adult female offspring and speculated the enhancement of cholinergic neurotransmission and synaptogenesis as potential molecular events underlying the neurobehavioral effects.

Dibenzodioxinas Policloradas , Efectos Tardíos de la Exposición Prenatal , Animales , Femenino , Humanos , Lactancia , Exposición Materna/efectos adversos , Ratones , Ratones Endogámicos C57BL , Dibenzodioxinas Policloradas/toxicidad , Embarazo , Efectos Tardíos de la Exposición Prenatal/inducido químicamente
Sci Total Environ ; 754: 142085, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-32898782


BACKGROUND: Endocrine-disrupting chemicals (EDCs) are considered to be related to diabetes, but studies of the association between phenolic EDCs and gestational diabetes mellitus (GDM) are limited. OBJECTIVES: To assess associations of maternal urinary bisphenol A (BPA), nonylphenol (NP), and 2-tert-octylphenol (2-t-OP) with GDM occurrence. METHODS: A cross-sectional study was performed among 390 Chinese women at 24-28 weeks of gestation. GDM was diagnosed with a 2-h 75-g oral glucose tolerance test (OGTT). BPA, NP, and 2-t-OP concentrations were determined in urine samples. Linear and logistic regression tests evaluated associations of BPA, NP, and 2-t-OP with blood glucose levels and GDM prevalence. RESULTS: The 2-t-OP concentrations in GDM patients were significantly higher than in non-GDM women with median values of 2.23 µg/g Cr and 1.79 µg/g Cr, respectively. No significant difference was observed in BPA and NP. Urinary 2-t-OP was positively associated with blood glucose levels after adjustment for several confounding factors and urinary BPA and NP. Higher 2-t-OP levels were associated with higher odds of GDM (OR: 5.78; 95% CI: 2.04, 16.37), whereas higher NP levels were associated with lower odds (OR: 0.22; 95% CI: 0.05, 0.85) in the adjusted models. In addition, compared to the first quartile of 2-t-OP, the adjusted odds ratios (ORs) and 95% confidence intervals (95% CIs) for GDM in the second, third, and fourth quartiles were 2.81 (1.23, 6.42), 3.01 (1.30, 6.93), and 5.49 (2.24, 13.46), respectively. CONCLUSION: Our study indicates that, for the first time to our knowledge, exposure to 2-t-OP is associated with a higher risk of GDM. However, higher NP exposure is associated with lower GDM risk. Further studies are necessary to affirm the associations of 2-t-OP and NP with GDM, and to elucidate the causality of these findings.

Diabetes Gestacional , Disruptores Endocrinos , Glucemia , Estudios Transversales , Diabetes Gestacional/inducido químicamente , Diabetes Gestacional/epidemiología , Estrógenos , Femenino , Humanos , Embarazo
Sci Total Environ ; 754: 142167, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-32916497


BACKGROUND: Previous studies on environmental pollutant exposure during pregnancy have mostly focused on individual chemical substances or single urine measurements. Thus, our understanding of the potential cumulative or interactive effects of exposure is limited. OBJECTIVE: We aimed to ascertain the characteristics and predictors of exposure to environmental chemicals over three trimesters among pregnant women. METHODS: We measured the concentrations of 34 chemicals in spot urine samples provided by 745 participants in their early, middle, and late pregnancy. We calculated Spearman correlation coefficients (SCC) between exposure levels of multiple chemicals in each trimester. K-means clustering and principal components analysis (PCA) were applied to classify the populations and reduce data dimensionality. We used generalized linear models (GLM) to confirm predictors of each cluster and principal component. RESULTS: SCC showed that the correlations of chemical concentrations from the same classes were higher than those among concentrations of different classes. Cluster analysis categorized participants into three clusters, and each cluster represented different chemical concentrations. We restricted the principal components to six, which explained more than 50% of the data variations. Several physiological, socio-demographic factors, and behavior patterns were related to different clusters and principal components. CONCLUSION: Distinct exposure patterns and dominant exposure components of multiple environmental chemicals among pregnant women might help research the potential health effects of exposure to chemical mixtures and develop relevant public health interventions.

Contaminantes Ambientales , China , Análisis por Conglomerados , Exposición a Riesgos Ambientales/análisis , Femenino , Humanos , Embarazo , Trimestres del Embarazo
Chemosphere ; 262: 127792, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-32805656


Tebuconazole is a triazole fungicide, used in agriculture to treat phytopathogenic fungi, and as a biocide, has been reported to be related to reproductive and developmental toxicity. The purpose of this study was to investigate the effect of tebuconazole exposure on rat fetal Leydig cells and fetal testis during pregnancy. Pregnant Sprague-Dawley rats were randomly divided into 4 groups, daily gavaged with corn oil (as a control), 25, 50, and 100 mg/kg body weight tebuconazole for 10 days (from the 12th day of pregnancy). Tebuconazole increased fetal serum testosterone and progesterone levels at a dose of 100 mg/kg. Exposure to 100 mg/kg tebuconazole significantly caused an increase in the number of fetal Leydig cells per testis without inducing cell aggregation. Tebuconazole up-regulated the expression of Star, Cyp11a1, Hsd17b3, and Fshr and their proteins. Further investigation found that tebuconazole caused increased phosphorylation of AKT1, ERK1/2, and mTOR, the level of BCL2, as well as the decrease of Beclin1, LC3B, and BAX, which may contribute to the fetal Leydig cell autophagy and proliferation. In conclusion, in utero exposure of tebuconazole causes the proliferation of fetal Leydig cells.

Fungicidas Industriales/toxicidad , Células Intersticiales del Testículo/efectos de los fármacos , Efectos Tardíos de la Exposición Prenatal/inducido químicamente , Reproducción/efectos de los fármacos , Triazoles/toxicidad , Animales , Femenino , Células Intersticiales del Testículo/metabolismo , Masculino , Fosfoproteínas/genética , Fosforilación , Embarazo , Efectos Tardíos de la Exposición Prenatal/patología , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Testículo/efectos de los fármacos , Testículo/embriología , Testículo/patología , Testosterona/sangre , Regulación hacia Arriba
Chemosphere ; 262: 128009, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33182144


Increasing evidence has highlighted the critical role of early life environment in shaping the future health outcomes of individuals in subsequent generations. Bisphenol S (BPS) has been widely used as a substitute for various plastic materials due to the limited application of Bisphenol A (BPA) which is an endocrine disruptor. However, the lack of efficient evaluation of BPS leaves doubts about the relevant substitute of BPA. Few studies of transgenerational inheritance have examined the effects of environmental exposures to endocrine disruptors on the immune system. In this study, we analyzed the transgenerational effects of BPS on intestinal inflammation and its consequence in metabolism. In this study, only F0 pregnant mice were exposed to BPS (1.5 µg/kg bw/day) from gestational day 0 until weaning of offspring. In this work, both F1 and F2 male offspring developed an inflammatory response in the ileum and colon at adulthood after F0 mothers were exposed to BPS; this phenomenon disappeared in F3. This inflammatory response in F1 male offspring is associated with a significant decrease of blood cholesterol without modification of metabolic status. Further, in F3 offspring male, the decrease of gut inflammatory response is associated with a decrease of fat weight and with an increase of blood glucose and cholesterol level. A sex-specific profile is observed in female offspring. We also observed that early life exposure to BPS was associated with strong abnormal intestinal immune status. The study presented here demonstrates that the immune system, like other organ systems, is vulnerable to transgenerational effects caused by environmental exposures.

Citocinas/análisis , Disruptores Endocrinos/toxicidad , Intestinos/inmunología , Fenoles/toxicidad , Efectos Tardíos de la Exposición Prenatal/inducido químicamente , Sulfonas/toxicidad , Animales , Glucemia/análisis , Peso Corporal/efectos de los fármacos , Heces/química , Femenino , Inflamación , Intestinos/crecimiento & desarrollo , Masculino , Ratones , Ratones Endogámicos C57BL , Embarazo , Efectos Tardíos de la Exposición Prenatal/inmunología
Rev. enferm. UERJ ; 28: e36283, jan.-dez. 2020.
Artículo en Inglés, Portugués | LILACS, BDENF - Enfermería | ID: biblio-1103400


Objetivo: analisar a influência das composições familiares na ocorrência da gravidez na adolescência. Método:estudo caso-controle, realizado com 74 gestantes adolescentes, grupo de casos, e 74 adultas jovens sem história pregressa de gravidez na adolescência, grupo controle, pareadas pela variável renda familiar. Os dados foram coletados por meio de entrevistas estruturadas realizadas no período deagosto a outubro de 2016 em Cuiabá, Mato Grosso, e em seguida analisados pelos métodos estatísticos descritivo e inferencial. Resultados: identificou-se associação entre a ocorrência do desfecho com pertencer a famílias não nucleares, não permanecer a mesma família durante a infância e adolescência, e a constituição de uma família própria no período da adolescência. Conclusão:verificou-se que adolescentes inseridas em famílias não nucleares estão mais expostas a fatores de risco para ocorrência da gravidez na adolescência, quando comparadas às jovensprovenientes de famílias com ambos os pais.

Objective: to analyze the influence of family compositions in the occurrence of pregnancy in adolescence. Method:this is a case-control study performed with 74 pregnant adolescents, group of cases, and 74 young adults without background history of pregnancy during adolescence, group control, paired by family income. Data were collected through structured interviews conducted in the period from August to October 2016 in Cuiabá, Mato Grosso, and then analyzed by descriptive and inferential statistical methods. Results:we identified an association between the occurrence of the outcome and the belonging to non-nuclear families, as well as the non-belonging to the same family during childhood and adolescence, besides the constitution of an own family in the period of adolescence. Conclusion: checked that adolescents inserted in nonnuclear families are more exposed to risk factors for the occurrence of pregnancy in adolescence when compared to young people coming from families with both parents.

Objetivo: analizar la influencia de las composiciones familiares en la ocurrencia del embarazo adolescente. Método: estudio caso-control efectuado con 74 adolescentes embarazadas, grupo de casos, y 74 jóvenes adultas sin historia anterior de embarazo en la adolescencia, grupo de control, agrupadas por sus ingresos familiares. Los datos se recopilaron mediante entrevistas estructuradas conducidas en el periodo de agosto a octubre de 2016 en Cuiabá, Mato Grosso, y posteriormente analizados por los métodos estadísticos descriptivo e inferencial. Resultados:se identificó una asociación entre la ocurrencia del desenlace y la pertenencia a las familias no nucleares, no permanencia en la misma familia durante niñez y adolescencia, y la constitución de una familia propia en el periodo de la adolescencia. Conclusion: comprobado eso que las adolescentes insertadas en familias no nucleares están más expuestas a los factores de riesgo para la ocurrencia del embarazo adolescente en comparación con las jóvenes provenientes de familias con ambos padres biológicos.

Humanos , Femenino , Embarazo , Adolescente , Adulto Joven , Embarazo en Adolescencia/psicología , Composición Familiar , Factores de Riesgo , Conducta del Adolescente/psicología , Relaciones Familiares/psicología , Brasil/epidemiología , Núcleo Familiar/psicología , Estudios de Casos y Controles , Relaciones Padre-Hijo , Relaciones Madre-Hijo
Rev. enferm. UERJ ; 28: e43407, jan.-dez. 2020.
Artículo en Inglés, Portugués | LILACS, BDENF - Enfermería | ID: biblio-1119599


Objetivo: analisar as representações sociais da presença do genitor no pré-natal para as mulheres gestantes. Método: estudo descritivo e qualitativo, fundamentado na Teoria da Representações Sociais. Contribuíram com o estudo 28 gestantes que realizavam as consultas do pré-natal e responderam a um roteiro de entrevista em profundidade contendo três questões abertas, cujas respostas foram submetidas à Análise de Conteúdo Lexical, possibilitada pelo software IRAMUTEQ. Resultados: a análise aponta a palavra 'não" como a mais latente no sistema cognitivo das gestantes, sendo percebida a alta frequência no Dendograma de Classes, presença no eixo de intersecção entre as ordenadas e abscissas no Mapa Fatorial de Correspondência, além de ser central e fazer as maiores forças de conexidade com as demais palavras na árvore máxima de similitude. Conclusão: as representações sociais das gestantes sobre a presença do genitor durante as consultas de pré-natal foram elaboradas a partir da negação, evidenciadas nos discursos do grupo no termo "não".

Objective: to examine social representations held by pregnant women of the fathers' presence in prenatal care. Method: this qualitative, descriptive study, based on Social Representations Theory, involved 28 pregnant women undergoing prenatal consultations, who answered an in-depth, scripted interview containing three open questions. Their responses were analyzed using Lexical Content Analysis, made possible by IRAMUTEQ software. Results: the analysis pointed to the word "no" as the most latent in the pregnant women's cognitive system: it was found at high frequency in the Dendrogram of Classes, was present at the axis of intersection between ordinates and abscissas on the Factorial Correspondence Map, besides being central and showing strongest connectedness with the other words in the similarity tree. Conclusion: the pregnant women's social representations of the fathers' presence at prenatal appointments were elaborated on the basis of denial, evidenced in the group's discourse in the term "no".

Objetivo: analizar las representaciones sociales que tienen las mujeres embarazadas sobre la presencia del padre en la atención prenatal. Método: este estudio cualitativo, descriptivo, basado en la Teoría de las Representaciones Sociales, involucró a 28 gestantes en consulta prenatal, quienes respondieron una entrevista en profundidad y guionizada que contenía tres preguntas abiertas. Sus respuestas se analizaron mediante el análisis de contenido léxico, posible gracias al software IRAMUTEQ. Resultados: el análisis apuntó a la palabra "no" como la más latente en el sistema cognitivo de la gestante: se encontró con alta frecuencia en el Dendrograma de Clases, estuvo presente en el eje de intersección entre ordenadas y abscisas en el Mapa de Correspondencia Factorial , además de ser central y mostrar una conexión más fuerte con las otras palabras en el árbol de similitudes. Conclusión: las representaciones sociales de las mujeres embarazadas sobre la presencia de los padres en las citas prenatales se elaboraron sobre la base de la negación, evidenciada en el discurso del grupo en el término "no".

Humanos , Femenino , Embarazo , Adolescente , Adulto , Adulto Joven , Atención Prenatal/psicología , Mujeres Embarazadas/psicología , Relaciones Padre-Hijo , Padre/psicología , Brasil , Epidemiología Descriptiva , Investigación Cualitativa , Teoría Social , Enfermería Obstétrica
Goiânia; s.n; 06 nov. 2020. 1-23 p. ilus, tab, mapas, graf.(Boletim Epidemiológico Covid-19, 31).
Monografía en Portugués | Coleciona SUS, CONASS, SES-GO | ID: biblio-1128737


O Boletim epidemiológico COVID-19, da Secretaria de Estado da Saúde do Estado de Goiás (SES-GO) objetiva apresentar as informações oficiais da situação epidemiológica referente ao período compreendido entre 04 de fevereiro à 31 de outubro de 2020. No Estado de Goiás, 255.744 (35,3%) foram confirmados sendo 244.877 (95,8%) por critério laboratorial, 7.152 (2,8%) pelo critério clínico epidemiológico, 1.054 (0,4%) por critério clínico-imagem e 2.106 (0,8%) pelo critério clínico, 226.894 (31,3%) foram descartados e 242.166 (33,4%) continuam como suspeitos. Na SE 44 foram realizados 601 testes (26,9% a menos do que na SE anterior), sendo 120 (19,9%) positivos e 480 (79,9%) negativos e 1 (0,2) inconclusivo

The epidemiological bulletin COVID-19, of the State Department of Health of the State of Goiás (SES-GO) aims to present the official information of the epidemiological situation referring to the period from February 4 to October 31, 2020. In the State of Goiás, 255,744 (35.3%) were confirmed, with 244,877 (95.8%) by laboratory criterion, 7,152 (2.8%) epidemiological clinical criterion, 1,054 (0.4%) by clinical-imaging criterion and 2,106 (0.8%) by clinical criterion, 226,894 (31.3%) were discarded and 242,166 (33.4%) remain as suspects. In the SE 44, 601 tests were performed (26.9% less than in the previous SE), 120 (19.9%) positive and 480 (79.9%) negative and 1 (0.2) inconclusive

Humanos , Masculino , Femenino , Embarazo , Recién Nacido , Lactante , Preescolar , Niño , Adolescente , Adulto , Persona de Mediana Edad , Anciano , Anciano de 80 o más Años , Adulto Joven , Neumonía Viral/epidemiología , Infecciones por Coronavirus/epidemiología , Pandemias , Betacoronavirus , Neumonía Viral/diagnóstico , Neumonía Viral/mortalidad , Brasil/epidemiología , Incidencia , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/mortalidad , Monitoreo Epidemiológico
Goiânia; s.n; Nov. 13, 2020. 1-22 p. ilus, mapas, graf, tab.(Boletim Epidemiológico Covid-19, 32).
Monografía en Portugués | Coleciona SUS, CONASS, SES-GO | ID: biblio-1128966


O Boletim epidemiológico COVID-19, da Secretaria de Estado da Saúde do Estado de Goiás (SES-GO) objetiva apresentar as informações oficiais da situação epidemiológica do Estado de Goiás referente ao período compreendido entre 04 de fevereiro a 07 de novembro de 2020. Nesta última semana epidemiológica (SE 45) houve a confirmação de 5.895 casos novos, representando uma redução de 29%, inferior a redução observada no Brasil, 75%. No Estado, 261.639 (35,3%) foram confirmados sendo 250.066 (95,6%) por critério laboratorial, 7.544 (2,9%) pelo critério clínico epidemiológico, 1.157 (0,4%) por critério clínico-imagem e 2.262 (0,9%) pelo critério clínico, 235.601 (31,8%) foram descartados e 243.031 (32,8%) continuam como suspeitos. Na SE 45 foram realizados 460 testes (23,4% a menos do que na SE anterior), sendo 80 (17,3%) positivos, 379 (82,3%) negativos e 2 (0,4) inconclusivos

The epidemiological bulletin COVID-19, of the State Department of Health of the State of Goiás (SES-GO) aims to present the official information of the epidemiological situation of the State of Goiás for the period from February 4 to November 7, 2020. In this last epidemiological week (SE 45) there was confirmation of 5,895 new cases, representing a reduction of 29%, lower than the reduction observed in Brazil, 75%. In the State, 261,639 (35.3%) were confirmed being 250,066 (95.6%) by laboratory criterion, 7,544 (2.9%) by epidemiological clinical criterion, 1,157 (0.4%) by clinical-imaging criterion and 2,262 (0.9%) by clinical criterion, 235,601 (31.8%) were discarded and 243,031 (32.8%) remain as suspects. In the SE 45, 460 tests were performed (23.4% less than in the previous SE), 80 (17.3%) positive, 379 (82.3%) negative and 2 (0.4) inconclusive

Humanos , Embarazo , Recién Nacido , Lactante , Preescolar , Niño , Adolescente , Adulto , Persona de Mediana Edad , Anciano , Anciano de 80 o más Años , Adulto Joven , Neumonía Viral/epidemiología , Infecciones por Coronavirus/epidemiología , Pandemias , Betacoronavirus , Neumonía Viral/diagnóstico , Neumonía Viral/mortalidad , Brasil/epidemiología , Incidencia , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/mortalidad , Monitoreo Epidemiológico
Rev. bioét. derecho ; (50): 167-188, nov. 2020. graf
Artículo en Español | IBECS | ID: ibc-191352


La llegada de la pandemia por coronavirus SARS-CoV-2 (la enfermedad de la COVID-19) a España también ha repercutido en el acceso parental a las Unidades de Cuidados Intensivos Neonatales (UCIN). Al comienzo de la pandemia se tomaron las medidas que, fundamentadas en criterios de salud pública, se consideraron necesarias. A medida que avanzaba el conocimiento científico sobre la COVID-19, y en base a los nuevos datos obtenidos, se comenzaron a adaptar las medidas inicialmente establecidas. Sin embargo, creemos que la adaptación de alguno de estos protocolos, en concreto el del acceso parental a las UCIN, no ha sido la idónea desde el punto de vista bioético y del interés superior del menor. A lo largo de este artículo exponemos esas insuficiencias

The arrival of the SARS-CoV-2 coronavirus pandemic (COVID-19 disease) in Spain has also had an impact on parental access to Neonatal Intensive Care Units (NICU). At the beginning of the pandemic, measures were taken that, based on public health criteria, and were considered necessary. As scientific knowledge about COVID-19 advanced, and based on the new data obtained, the measures initially established began to be adapted. However, we believe that the adaptation of some of these protocols, specifically that of parental access to NICU, has not been the ideal one from the bioethical point of view and in the best interest of the minor. Throughout this article we expose these insufficiencies

L'arribada de la pandèmia per coronavirus SARS-CoV-2 (la malaltia de la COVID-19) a Espanya també ha repercutit en l'accés parental a les Unitats de Cures Intensives Neonatals (UCIN). A l'inici de la pandèmia es van prendre les mesures que, fonamentades en criteris de salut pública, es van considerar necessàries. A mesura que avançava el coneixement científic sobre la COVID-19, I en base a les noves dades obtingudes, es van començar a adaptar, les mesures inicialment establertes. No obstant això, creiem que l'adaptació d'algun d'aquests protocols, en concret el de l'accés parental a les UCIN, no ha estat la idònia des del punt de vista bioètic I de l'interès superior del menor. Al llarg d'aquest article exposem aquestes insuficiències

Humanos , Masculino , Femenino , Embarazo , Recién Nacido , Cuidado Intensivo Neonatal/ética , Infecciones por Coronavirus/terapia , Neumonía Viral/terapia , Pandemias , Padres , Aislamiento de Pacientes , Chaperones Médicos/ética , Chaperones Médicos/legislación & jurisprudencia , Humanización de la Atención
Wiad Lek ; 73(9 cz. 2): 1895-1899, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33148830


OBJECTIVE: The aim: To determine the morphometric parameters of the parenchymal and stromal liver components of healthy newborns. PATIENTS AND METHODS: Material and methods: The morphometric investigation included 45 liver tissue biopsies of healthy newborns. All morphometric parameters of the parenchymal and stromal liver components were calculated using the Avtandilov microscopic morphometric grid. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found on the corresponding types of parenchymal and stromal liver components was calculated. In every case, it was selected 10 random microscopic areas and then all data were obtained, calculated and presented as percentages. RESULTS: Results: Morphometric parameters of hepatocytes: mononuclear hepatocytes - 93.5±7.1 %, two-nuclear hepatocytes - 6.5±1.2 %, BMHC (bi-/mononuclear hepatocytes coefficient) - 0.06±0.01, hepatocytes with fat vacuoles - 0.5±0.2 %. Parenchymal and stromal liver components: parenchyma - 74.2±4.3 %, stroma (including blood vessels and bile ducts) - 25.8±2.6 %, SPI (stroma/parenchyma index) - 0.34±0.01. Morphometric parameters of all of the liver components: hepatocytes - 74.2±4.3 %, portal tracts - 3.1±0.6 %, central veins - 9.3±1.4 %, sinusoids - 10.5±1.3 %, bile ducts - 2.9±0.2 %. Expression level parameters: fibronectin - 17.3±2.5 %, collagen type I - 9.7±1.9 %, collagen type III - 10.1±0.9 %, collagen type IV - 5.9±0.2 %. Parameters of liver fibrosis biomarkers: APRI (index) - 0.19±0.01, а FIB-4 (index) - 0.022±0.001. CONCLUSION: Conclusions: The morphometric parameters of the parenchymal and stromal liver components of healthy newborns can be used as a control group in the study of any pathological conditions of the liver of newborns.

Tejido Conectivo , Madres , Femenino , Hepatocitos , Humanos , Recién Nacido , Cirrosis Hepática , Embarazo