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1.
J Cell Sci ; 135(5)2022 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-33414166

RESUMEN

Ferroptosis is a regulated, non-apoptotic form of cell death, characterized by hydroxy-peroxidation of discrete phospholipid hydroperoxides, particularly hydroperoxyl (Hp) forms of arachidonoyl- and adrenoyl-phosphatidylethanolamine, with a downstream cascade of oxidative damage to membrane lipids, proteins and DNA, culminating in cell death. We recently showed that human trophoblasts are particularly sensitive to ferroptosis caused by depletion or inhibition of glutathione peroxidase 4 (GPX4) or the lipase PLA2G6. Here, we show that trophoblastic ferroptosis is accompanied by a dramatic change in the trophoblast plasma membrane, with macro-blebbing and vesiculation. Immunofluorescence revealed that ferroptotic cell-derived blebs stained positive for F-actin, but negative for cytoplasmic organelle markers. Transfer of conditioned medium that contained detached macrovesicles or co-culture of wild-type target cells with blebbing cells did not stimulate ferroptosis in target cells. Molecular modeling showed that the presence of Hp-phosphatidylethanolamine in the cell membrane promoted its cell ability to be stretched. Together, our data establish that membrane macro-blebbing is characteristic of trophoblast ferroptosis and can serve as a useful marker of this process. Whether or not these blebs are physiologically functional remains to be established.


Asunto(s)
Ferroptosis , Femenino , Humanos , Peroxidación de Lípido , Fosfolípido Hidroperóxido Glutatión Peroxidasa , Placenta , Embarazo , Trofoblastos
2.
BMC Genomics ; 22(1): 489, 2021 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-34193041

RESUMEN

BACKGROUND: Preterm birth is the leading cause of neonatal morbidity and mortality, but research efforts in neonatology are complicated due to the unavailability of large volume blood samples. Whole blood assays can be used to overcome this problem by performing both functional and gene expression studies using small amounts of blood. Gene expression studies using RT-qPCR estimate mRNA-levels of target genes normalized to reference genes. The goal of this study was to identify and validate stable reference genes applicable to cord blood samples obtained from developing neonates of different gestational age groups as well as to adult peripheral blood samples. Eight reference gene candidates (ACTB, B2M, GAPDH, GUSB, HPRT, PPIB, RPLP0, RPL13) were analyzed using the three published software algorithms Bestkeeper, GeNorm and NormFinder. RESULTS: A normalization factor consisting of ACTB and PPIB allows for comparative expression analyses of neonatal samples from different gestational age groups. Normalization factors consisting of GAPDH and PPIB or ACTB and GAPDH are suitable when samples from preterm and full-term neonates and adults are compared. However, all candidate reference genes except RPLP0 exhibited significant intergroup gene expression variance and a higher gene expression towards an older age which resulted in a small but statistically significant systematic bias. Systematic analysis of RNA-seq data revealed new reference gene candidates with potentially superior stability. CONCLUSIONS: The current study identified suitable normalization factors and proposed the use of the additional single gene RPLP0 to avoid systematic bias. This combination will enable comparative analyses not only between neonates of different gestational ages, but also between neonates and adults, as it facilitates more detailed investigations of developmental gene expression changes. The use of software algorithms did not prevent unintended systematic bias. This generally highlights the need for careful validation of such results to prevent false interpretation of potential age-dependent changes in gene expression. To identify the most stable reference genes in the future, RNA-seq based global approaches are recommended.


Asunto(s)
Sangre Fetal , Nacimiento Prematuro , Adulto , Anciano , Femenino , Expresión Génica , Perfilación de la Expresión Génica , Humanos , Recién Nacido , Proteínas de Neoplasias , Embarazo , Reacción en Cadena en Tiempo Real de la Polimerasa , Estándares de Referencia , Proteínas Ribosómicas/genética
3.
Nihon Yakurigaku Zasshi ; 156(4): 244-249, 2021.
Artículo en Japonés | MEDLINE | ID: mdl-34193705

RESUMEN

Evidence from our recent studies points to the notion that adult neurogenesis in the hippocampus may serve as a sensitive endpoint to detect developmental neurotoxicity. Adult neurogenesis is the postnatal process of continued generation of new neurons through the adult stage in the brain. Monitoring of granule cell lineages generated from the subgranular zone and γ-aminobutyric acid (GABA)-ergic interneurons in the hilus of the dentate gyrus as major players consisting of hippocampal neurogenesis is effective for detecting target cell populations of developmental neurotoxicants. Especially, reelin-expressing GABAergic interneurons are a useful marker to predict disruption of migration and correct positioning of newborn neurons following disruption of neurogenesis. Because axon terminal toxicants target granule cell lineage population showing neurite outgrowth, there may be common target mechanisms between the developmental and adult-type neurotoxicity. Because adult neurogenesis continues through the adult stage, developmental neurotoxicity could be detected in regular toxicity studies, such as in a 28-day repeated dose study. Alternatively, adult-type neurotoxicity could be detected by measuring the cellular responses in neurogenesis. Moreover, it should be stressed that there may be epigenetic toxicity mechanisms to affect the process of neurogenesis involving neuronal stem cells and interneuron subpopulations, showing continued disruption through the adult stage. These findings suggest that hippocampal neurogenesis is considered to be a critical target of neurotoxicity of both developmental and adult types.


Asunto(s)
Efectos Tardíos de la Exposición Prenatal , Apoptosis , Giro Dentado , Femenino , Hipocampo , Humanos , Recién Nacido , Interneuronas , Neurogénesis , Embarazo
5.
BMJ Case Rep ; 14(7)2021 Jul 05.
Artículo en Inglés | MEDLINE | ID: mdl-34226253

RESUMEN

A 49-year-old woman, G8P7, presented with 1 week of worsening vaginal bleeding and abdominal cramps in the setting of a recently discovered unplanned pregnancy. Vaginal ultrasound findings and a significantly elevated human chorionic gonadotropin (hCG) level were concerning for molar pregnancy. She developed signs of hyperthyroidism on the night of admission, for which the endocrinology team was consulted. Laboratory data were consistent with hyperthyroidism. The patient was believed to have thyrotoxicosis secondary to molar pregnancy with concern for impending thyroid storm. Her mental health disorder and bacteraemia made taking care of her further challenging. She was started on a beta-blocker, antithyroid agent and intravenous corticosteroids. She underwent an uncomplicated suction dilation and curettage (D&C), with resolution of her symptoms a few days after. At a follow-up appointment, the patient continued to be asymptomatic and was feeling well.


Asunto(s)
Mola Hidatiforme , Crisis Tiroidea , Tirotoxicosis , Antitiroideos , Femenino , Humanos , Mola Hidatiforme/diagnóstico , Mola Hidatiforme/diagnóstico por imagen , Persona de Mediana Edad , Embarazo , Tirotoxicosis/complicaciones , Tirotoxicosis/diagnóstico , Tirotoxicosis/tratamiento farmacológico , Legrado por Aspiración
6.
BMJ Case Rep ; 14(7)2021 Jul 05.
Artículo en Inglés | MEDLINE | ID: mdl-34226256

RESUMEN

We document a case of a 24-year-old gravida 2, para 1 (1001), on her second trimester, who was referred to the surgical oncology service for a palpable right hemiabdominal mass. She previously underwent wide excision of a retroperitoneal atypical lipomatous tumour 2 years ago. Her current case was successfully managed by a multidisciplinary team of doctors from the preoperative phase, the actual surgery up to the adjuvant treatment. She had a two-staged surgery: an elective lower-segment caesarean section at 34 weeks age of gestation for which she delivered a healthy baby girl and, 2 weeks after, she had excision of the huge retroperitoneal tumour recurrence. Histopathology revealed a 35 cm×25 cm×22 cm dedifferentiated liposarcoma, for which she was started on adjuvant systemic chemotherapy.


Asunto(s)
Liposarcoma , Neoplasias Retroperitoneales , Adulto , Cesárea , Femenino , Humanos , Recién Nacido , Liposarcoma/diagnóstico por imagen , Liposarcoma/cirugía , Recurrencia Local de Neoplasia/diagnóstico por imagen , Recurrencia Local de Neoplasia/cirugía , Embarazo , Segundo Trimestre del Embarazo , Neoplasias Retroperitoneales/diagnóstico por imagen , Neoplasias Retroperitoneales/cirugía , Adulto Joven
7.
BMJ Case Rep ; 14(7)2021 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-34233868

RESUMEN

Intrahepatic cholestasis of pregnancy (ICP) generally presents in the third trimester with pruritus without a rash, characterised by elevated bile acids, with or without transaminitis and hyperbilirubinaemia. Risk factors include a family history of cholestasis, South Asian ethnicity, multifetal gestation, in vitro fertilisation (IVF) and history of hepatitis or biliary disorders.IVF involves the use of high dose gonadotropin stimulation and human chorionic gonadotropin trigger. High doses of progesterone supplementation are additionally given after embryo transfer. The increase in oestrogen and progesterone levels early on in the pregnancy is a possible explanation for the development of ICP in IVF pregnancies at earlier gestations.We present a rare case of iatrogenic ICP presenting in the first trimester in a pregnancy conceived by IVF. Unlike other cases reported, our patient did not have recurrence of ICP in the third trimester, and also had no history of ICP in her first pregnancy.


Asunto(s)
Colestasis Intrahepática , Complicaciones del Embarazo , Colestasis Intrahepática/diagnóstico , Femenino , Fertilización In Vitro , Humanos , Embarazo , Primer Trimestre del Embarazo
9.
BMJ Case Rep ; 14(7)2021 Jul 09.
Artículo en Inglés | MEDLINE | ID: mdl-34244192

RESUMEN

Uterine scar dehiscence with underlying placenta is often misdiagnosed as placenta accreta spectrum both prenatally and intraoperatively due to the absence of myometrial tissue in the area. Misdiagnosis generates obstetric anxiety and results in overtreatment which carries a risk of iatrogenic injury. We present a case of the antenatal diagnosis of uterine dehiscence in a 36-year-old woman with a history of two caesarean deliveries and a low-lying placenta. We further describe the sonographic features useful for differentiating this condition from placenta accreta spectrum in instances where the placenta lies under an area of full thickness uterine scar dehiscence.


Asunto(s)
Placenta Accreta , Placenta Previa , Adulto , Cesárea/efectos adversos , Femenino , Humanos , Placenta/diagnóstico por imagen , Placenta Accreta/diagnóstico por imagen , Placenta Accreta/cirugía , Placenta Previa/diagnóstico por imagen , Placenta Previa/cirugía , Embarazo , Diagnóstico Prenatal , Ultrasonografía Prenatal
11.
Isr Med Assoc J ; 23(7): 437-440, 2021 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-34251127

RESUMEN

BACKGROUND: Pregnant women with Marfan syndrome (MS) have a high risk of aortic dissection around delivery and their optimal management requires a multi-disciplinary approach, including proper cardio-obstetric care and adequate pain management during labor, which may be difficult due to the high prevalence of dural ectasia (DE) in these patients. OBJECTIVES: To evaluate the multidisciplinary management of MS patients during labor. METHODS: Nineteen pregnant women (31 pregnancies) with MS were followed by a multi-disciplinary team (cardiologist, obstetrician, anesthesiologist) prior to delivery. RESULTS: Two patients had kyphoscoliosis; none had previous spine surgery nor complaints compatible with DE. In eight pregnancies (7 patients), aortic root diameter (ARd) before pregnancy was 40 to 46 mm. In this high-risk group, one patient underwent elective termination, two underwent an urgent cesarean section (CS) under general anesthesia, and five had elective CS; two under general anesthesia (GA), and three under spinal anesthesia. In 23 pregnancies (12 patients), ARd was < 40 mm. In this non-high-risk group three pregnancies (1 patient) were electively terminated. Of the remaining 20 deliveries (11 patients), 14 were vaginal deliveries, 9 with epidural analgesia and 5 without. Six patients had a CS; four under GA and two2 under spinal anesthesia. There were no epidural placement failures and no failed responses. There were 2 cases of aortic dissection, unrelated to the anesthetic management. CONCLUSIONS: The optimal anesthetic strategy during labor in MS patients should be decided by a multi-disciplinary team. Anesthetic complications due to DE were not encountered during neuraxial block.


Asunto(s)
Anestesia Obstétrica , Aneurisma Disecante , Enfermedades de la Aorta , Parto Obstétrico , Síndrome de Marfan , Complicaciones del Trabajo de Parto , Complicaciones del Embarazo , Adulto , Anestesia Obstétrica/efectos adversos , Anestesia Obstétrica/métodos , Anestesia Obstétrica/estadística & datos numéricos , Aneurisma Disecante/diagnóstico , Aneurisma Disecante/prevención & control , Enfermedades de la Aorta/complicaciones , Enfermedades de la Aorta/diagnóstico , Enfermedades de la Aorta/etiología , Parto Obstétrico/efectos adversos , Parto Obstétrico/métodos , Parto Obstétrico/estadística & datos numéricos , Femenino , Humanos , Comunicación Interdisciplinaria , Israel/epidemiología , Síndrome de Marfan/complicaciones , Síndrome de Marfan/epidemiología , Síndrome de Marfan/fisiopatología , Monitoreo Fisiológico/métodos , Complicaciones del Trabajo de Parto/diagnóstico , Complicaciones del Trabajo de Parto/etiología , Complicaciones del Trabajo de Parto/prevención & control , Evaluación de Procesos y Resultados en Atención de Salud , Embarazo , Complicaciones del Embarazo/diagnóstico , Complicaciones del Embarazo/fisiopatología , Complicaciones del Embarazo/terapia , Resultado del Embarazo/epidemiología , Embarazo de Alto Riesgo
12.
JAMA ; 326(2): 154-164, 2021 07 13.
Artículo en Inglés | MEDLINE | ID: mdl-34255008

RESUMEN

Importance: There is limited information about trends in the treatment of opioid use disorder (OUD) among Medicaid enrollees. Objective: To examine the use of medications for OUD and potential indicators of quality of care in multiple states. Design, Setting, and Participants: Exploratory serial cross-sectional study of 1 024 301 Medicaid enrollees in 11 states aged 12 through 64 years (not eligible for Medicare) with International Classification of Diseases, Ninth Revision (ICD-9 or ICD-10) codes for OUD from 2014 through 2018. Each state used generalized estimating equations to estimate associations between enrollee characteristics and outcome measure prevalence, subsequently pooled to generate global estimates using random effects meta-analyses. Exposures: Calendar year, demographic characteristics, eligibility groups, and comorbidities. Main Outcomes and Measures: Use of medications for OUD (buprenorphine, methadone, or naltrexone); potential indicators of good quality (OUD medication continuity for 180 days, behavioral health counseling, urine drug tests); potential indicators of poor quality (prescribing of opioid analgesics and benzodiazepines). Results: In 2018, 41.7% of Medicaid enrollees with OUD were aged 21 through 34 years, 51.2% were female, 76.1% were non-Hispanic White, 50.7% were eligible through Medicaid expansion, and 50.6% had other substance use disorders. Prevalence of OUD increased in these 11 states from 3.3% (290 628 of 8 737 082) in 2014 to 5.0% (527 983 of 10 585 790) in 2018. The pooled prevalence of enrollees with OUD receiving medication treatment increased from 47.8% in 2014 (range across states, 35.3% to 74.5%) to 57.1% in 2018 (range, 45.7% to 71.7%). The overall prevalence of enrollees receiving 180 days of continuous medications for OUD did not significantly change from the 2014-2015 to 2017-2018 periods (-0.01 prevalence difference, 95% CI, -0.03 to 0.02) with state variability in trend (90% prediction interval, -0.08 to 0.06). Non-Hispanic Black enrollees had lower OUD medication use than White enrollees (prevalence ratio [PR], 0.72; 95% CI, 0.64 to 0.81; P < .001; 90% prediction interval, 0.52 to 1.00). Pregnant women had higher use of OUD medications (PR, 1.18; 95% CI, 1.11-1.25; P < .001; 90% prediction interval, 1.01-1.38) and medication continuity (PR, 1.14; 95% CI, 1.10-1.17, P < .001; 90% prediction interval, 1.06-1.22) than did other eligibility groups. Conclusions and Relevance: Among US Medicaid enrollees in 11 states, the prevalence of medication use for treatment of opioid use disorder increased from 2014 through 2018. The pattern in other states requires further research.


Asunto(s)
Antagonistas de Narcóticos/uso terapéutico , Tratamiento de Sustitución de Opiáceos/tendencias , Trastornos Relacionados con Opioides/tratamiento farmacológico , Adolescente , Adulto , Analgésicos Opioides/uso terapéutico , Buprenorfina/uso terapéutico , Niño , Estudios Transversales , Femenino , Humanos , Masculino , Medicaid , Metadona/uso terapéutico , Persona de Mediana Edad , Naltrexona/uso terapéutico , Embarazo , Complicaciones del Embarazo/tratamiento farmacológico , Estados Unidos , Adulto Joven
13.
Vet Clin North Am Equine Pract ; 37(2): 339-366, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-34243877

RESUMEN

Selected emergency conditions of male and female reproductive tracts in horses are described, including injuries affecting the external genitalia of male horses and emergent conditions arising during gestation in mares. Conditions affecting male horses are discussed in the context of breeding stallions, but kicks or other mechanisms of trauma in the groin can also affect geldings. Priapism, paraphimosis, trauma to the scrotum and testicles, and penile injury are discussed. In mares, traumatic vestibular injury, placentitis, hydropsic conditions, prepubic tendon and abdominal wall compromise, and uterine torsion are included. Clinical recognition of the problem, diagnostic procedures, and treatments are summarized.


Asunto(s)
Enfermedades de los Caballos/diagnóstico , Parafimosis/veterinaria , Priapismo/veterinaria , Reproducción , Animales , Cruzamiento , Urgencias Médicas/veterinaria , Femenino , Genitales Femeninos/lesiones , Genitales Masculinos/lesiones , Enfermedades de los Caballos/terapia , Caballos , Masculino , Parafimosis/diagnóstico , Parafimosis/terapia , Embarazo , Priapismo/diagnóstico , Priapismo/terapia , Heridas y Lesiones/veterinaria
14.
Vet Clin North Am Equine Pract ; 37(2): 367-405, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-34243878

RESUMEN

Selected conditions affecting broodmares are discussed, including arterial rupture, dystocia, foal support with ex utero intrapartum treatment, uterine prolapse, postpartum colic, the metritis/sepsis/systemic inflammatory response syndrome complex, and retained fetal membranes. Postpartum colic beyond third-stage labor contractions should prompt comprehensive evaluation for direct injuries to the reproductive tract or indirect injury of the intestinal tract. Mares with perforation or rupture of the uterus are typically recognized 1 to 3 days after foaling, with depression, fever, and leukopenia; laminitis and progression to founder can be fulminant. The same concerns are relevant in mares with retention of fetal membranes.


Asunto(s)
Distocia/veterinaria , Enfermedades de los Caballos/diagnóstico por imagen , Parálisis de la Parturienta/diagnóstico por imagen , Retención de la Placenta/veterinaria , Reproducción , Prolapso Uterino/veterinaria , Animales , Distocia/diagnóstico por imagen , Distocia/terapia , Diagnóstico por Imagen de Elasticidad/veterinaria , Urgencias Médicas/veterinaria , Membranas Extraembrionarias/diagnóstico por imagen , Femenino , Hemorragia/veterinaria , Enfermedades de los Caballos/terapia , Caballos , Parálisis de la Parturienta/terapia , Parto , Retención de la Placenta/diagnóstico por imagen , Retención de la Placenta/terapia , Periodo Posparto , Embarazo , Prolapso Uterino/diagnóstico por imagen , Prolapso Uterino/terapia , Útero/diagnóstico por imagen
15.
Nanoscale ; 13(28): 12279-12287, 2021 Jul 28.
Artículo en Inglés | MEDLINE | ID: mdl-34251003

RESUMEN

Rapid diagnosis of preeclampsia is necessary to ensure timely administration of appropriate care and prevent the potentially catastrophic complications of the condition affecting both mothers and babies. While the diagnostic superiority of angiogenic blood biomarkers such as placental growth factor has recently been demonstrated, there is an urgent need to develop point-of-care (PoC) technologies that allow rapid, quantitative, and accurate testing for these markers within local communities. Towards addressing this need, here we report on a fully integrated biodiagnostic platform based on nanoscale indium oxide field effect transistor (FET) sensors. The high-performance FET sensors are integrated with blood sample processing cartridges that minimize the need for operator intervention during the assay and eliminate the need for analytical equipment. Within 40 minutes and from 30 µL of blood, the FET platform could reliably measure PlGF with a limit of detection of 0.06 pg mL-1 and a five order of magnitudes dynamic range. Pilot clinical validation in four preeclamptic pregnancies confirmed that the accuracy and reliability of the FET platform, paving the way for further development to a much-needed point-of-care preeclampsia testing.


Asunto(s)
Preeclampsia , Biomarcadores , Femenino , Humanos , Factor de Crecimiento Placentario , Pruebas en el Punto de Atención , Preeclampsia/diagnóstico , Embarazo , Reproducibilidad de los Resultados
16.
Sci Rep ; 11(1): 14390, 2021 07 13.
Artículo en Inglés | MEDLINE | ID: mdl-34257394

RESUMEN

The coronavirus disease 2019 (COVID-19) pandemic affected people at all ages. Whereas pregnant women seemed to have a worse course of disease than age-matched non-pregnant women, the risk of feto-placental infection is low. Using a cohort of 66 COVID-19-positive women in late pregnancy, we correlated clinical parameters with disease severity, placental histopathology, and the expression of viral entry and Interferon-induced transmembrane (IFITM) antiviral transcripts. All newborns were negative for SARS-CoV-2. None of the demographic parameters or placental histopathological characteristics were associated with disease severity. The fetal-maternal transfer ratio for IgG against the N or S viral proteins was commonly less than one, as recently reported. We found that the expression level of placental ACE2, but not TMPRSS2 or Furin, was higher in women with severe COVID-19. Placental expression of IFITM1 and IFITM3, which have been implicated in antiviral response, was higher in participants with severe disease. We also showed that IFITM3 protein expression, which localized to early and late endosomes, was enhanced in severe COVID-19. Our data suggest an association between disease severity and placental SARS-CoV-2 processing and antiviral pathways, implying a role for these proteins in placental response to SARS-CoV-2.


Asunto(s)
COVID-19/metabolismo , Placenta/metabolismo , SARS-CoV-2/patogenicidad , Adulto , Enzima Convertidora de Angiotensina 2/metabolismo , Femenino , Furina/metabolismo , Humanos , Inmunoglobulina G/metabolismo , Transmisión Vertical de Enfermedad Infecciosa , Masculino , Proteínas de la Nucleocápside/metabolismo , Embarazo , Complicaciones Infecciosas del Embarazo/metabolismo , Complicaciones Infecciosas del Embarazo/virología , Serina Endopeptidasas/metabolismo , Glicoproteína de la Espiga del Coronavirus/metabolismo , Adulto Joven
17.
Pediatr Cardiol ; 42(6): 1405-1409, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-34258648

RESUMEN

The objective of this study was to explore a new method for the differential diagnosis between fetal double aortic arch (DAA) and right aortic arch with mirror-image branches (RAA-MB). Clinical data and prenatal echocardiographic features of the DAA (n = 22) and RAA-MB (n = 65) confirmed by postnatal or autopsy findings were analyzed retrospectively. The angles between the two aortic arches in the DAA group and between the right aortic arch and the mirror branch were measured. The differences between the two groups and differential diagnosis value of the angles were compared and analyzed based on the receiver operating characteristic curve. The proportion of left-sided ductal arteriosus (100%) was higher in the DAA group than that (32.3%) in the RAA-MB group, (P < 0.05). The proportion of conotruncal anomalies is higher in the RAA-MB group (64.6%) than in the DAA group (18.2%) (P < 0.05). There was a significant difference in the angles between the groups (DAA: 50.3° ± 8.3° vs. RAA-MB: 82.9° ± 13.8°) (P < 0.01). When the cutoff value was 62.8°, the sensitivity and specificity of the differential diagnosis were 95.5% and 96.9%, respectively. Distinguishing the angle measurement between DAA and RAA-MB is helpful in prenatal prognosis. We recommend a cutoff value of 62.8°.


Asunto(s)
Aorta Torácica/diagnóstico por imagen , Síndromes del Arco Aórtico/diagnóstico , Conducto Arterial/diagnóstico por imagen , Ecocardiografía/métodos , Ultrasonografía Prenatal/métodos , Anillo Vascular/diagnóstico , Adulto , Aorta Torácica/anomalías , Diagnóstico Diferencial , Femenino , Humanos , Masculino , Embarazo , Estudios Retrospectivos , Adulto Joven
18.
Sci Rep ; 11(1): 14448, 2021 07 14.
Artículo en Inglés | MEDLINE | ID: mdl-34262075

RESUMEN

Faces hold a substantial value for effective social interactions and sharing. Covering faces with masks, due to COVID-19 regulations, may lead to difficulties in using social signals, in particular, in individuals with neurodevelopmental conditions. Daily-life social participation of individuals who were born preterm is of immense importance for their quality of life. Here we examined face tuning in individuals (aged 12.79 ± 1.89 years) who were born preterm and exhibited signs of periventricular leukomalacia (PVL), a dominant form of brain injury in preterm birth survivors. For assessing the face sensitivity in this population, we implemented a recently developed experimental tool, a set of Face-n-Food images bordering on the style of Giuseppe Arcimboldo. The key benefit of these images is that single components do not trigger face processing. Although a coarse face schema is thought to be hardwired in the brain, former preterms exhibit substantial shortages in the face tuning not only compared with typically developing controls but also with individuals with autistic spectrum disorders. The lack of correlations between the face sensitivity and other cognitive abilities indicates that these deficits are domain-specific. This underscores impact of preterm birth sequelae for social functioning at large. Comparison of the findings with data in individuals with other neurodevelopmental and neuropsychiatric conditions provides novel insights into the origins of deficient face processing.


Asunto(s)
Encéfalo/fisiología , Reconocimiento Facial , Reconocimiento Visual de Modelos , Nacimiento Prematuro , Cognición Social , Adolescente , Trastorno del Espectro Autista , COVID-19 , Niño , Cognición , Neurociencia Cognitiva , Expresión Facial , Femenino , Humanos , Leucomalacia Periventricular , Embarazo , Calidad de Vida , Reconocimiento en Psicología/fisiología , Factores Sexuales , Conducta Social , Percepción Visual/fisiología
19.
Int J Mol Sci ; 22(13)2021 Jul 05.
Artículo en Inglés | MEDLINE | ID: mdl-34281280

RESUMEN

Cold shock Y-box binding protein-1 (YB-1) coordinates several molecular processes between the nucleus and the cytoplasm and plays a crucial role in cell function. Moreover, it is involved in cancer progression, invasion, and metastasis. As trophoblast cells share similar characteristics with cancer cells, we hypothesized that YB-1 might also be necessary for trophoblast functionality. In samples of patients with intrauterine growth restriction, YB-1 mRNA levels were decreased, while they were increased in preeclampsia and unchanged in spontaneous abortions when compared to normal pregnant controls. Studies with overexpression and downregulation of YB-1 were performed to assess the key trophoblast processes in two trophoblast cell lines HTR8/SVneo and JEG3. Overexpression of YB-1 or exposure of trophoblast cells to recombinant YB-1 caused enhanced proliferation, while knockdown of YB-1 lead to proliferative disadvantage in JEG3 or HTR8/SVneo cells. The invasion and migration properties were affected at different degrees among the trophoblast cell lines. Trophoblast expression of genes mediating migration, invasion, apoptosis, and inflammation was altered upon YB-1 downregulation. Moreover, IL-6 secretion was excessively increased in HTR8/SVneo. Ultimately, YB-1 directly binds to NF-κB enhancer mark in HTR8/SVneo cells. Our data show that YB-1 protein is important for trophoblast cell functioning and, when downregulated, leads to trophoblast disadvantage that at least in part is mediated by NF-κB.


Asunto(s)
Complicaciones del Embarazo/metabolismo , Trofoblastos/metabolismo , Aborto Espontáneo/genética , Aborto Espontáneo/metabolismo , Aborto Espontáneo/patología , Adulto , Apoptosis , Estudios de Casos y Controles , Línea Celular , Movimiento Celular , Proliferación Celular , Regulación hacia Abajo , Femenino , Retardo del Crecimiento Fetal/genética , Retardo del Crecimiento Fetal/metabolismo , Retardo del Crecimiento Fetal/patología , Técnicas de Silenciamiento del Gen , Humanos , Técnicas In Vitro , Masculino , FN-kappa B/metabolismo , Preeclampsia/genética , Preeclampsia/metabolismo , Preeclampsia/patología , Embarazo , Complicaciones del Embarazo/genética , Complicaciones del Embarazo/patología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Trofoblastos/patología , Regulación hacia Arriba , Proteína 1 de Unión a la Caja Y/antagonistas & inhibidores , Proteína 1 de Unión a la Caja Y/genética , Proteína 1 de Unión a la Caja Y/metabolismo , Adulto Joven
20.
BMC Pregnancy Childbirth ; 21(1): 515, 2021 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-34281501

RESUMEN

BACKGROUND: In light of the pandemic, pregnant women are particularly vulnerable to increased psychological distress and in need of imperative preventive measures. This study aimed to investigate the impact of the pandemic on mental health, lifestyle adaptations, and their determinants among pregnant women in the United Arab Emirates. METHODS: A survey was conducted electronically between June and August 2020. Pregnant women were recruited from prenatal clinics in the UAE and invited to participate in an online survey developed on Google Forms. The questionnaire included socio-demographic characteristics, the Impact of Event Scale- Revised, the Perceived Support Scale and lifestyle-related factors. RESULTS: A total of 384 pregnant women completed the questionnaire of whom 20.6% were in their 1st trimester, 46.1% in their 2nd and 33.3% in their 3rd trimester. The mean IES-R score for the respondents was 26.15 ± 13.55, corresponding to a mild stressful impact, which did not differ significantly among trimesters of pregnancy. Pregnant women expressed increased stress from staying home (64%), work (40%), feeling frightened (66%) and apprehensive (59%). Women reported increased support and sharing their feelings with family members (59%), mainly in the 1st and 3rd trimester of pregnancy (P < 0.05). There was a greater attention to mental health (48%), resting time (55.3%), and relaxing time (57.3%); while a decreased amount of time was spent engaging in physical activities (53.6%), which differed significantly between trimesters (P = 0.02). CONCLUSIONS: The COVID-19 pandemic was associated with a mild stressful impact among pregnant women in the UAE, braced by strong family support and self-care mental health behaviors.


Asunto(s)
Adaptación Fisiológica , Adaptación Psicológica , COVID-19 , Mujeres Embarazadas/psicología , Adulto , Control de Enfermedades Transmisibles , Estudios Transversales , Ejercicio Físico , Femenino , Conductas Relacionadas con la Salud , Humanos , Embarazo , Apoyo Social , Estrés Psicológico/etiología , Encuestas y Cuestionarios , Emiratos Árabes Unidos
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