Is it me or others who matter? The interplay between consumer values vis-à-vis status and affiliation motives as shapers of meat alternative interest.

This study is about the role of consumers' personal values (Self-enhancement, Openness to change, Self-transcendence, and Conservation) in consumers' interest towards meat alternatives. In addition, the underlying role of two social motives, status and group affiliation are analysed. A conceptual model with hypotheses was developed and validated, and the hypotheses were tested through PLS-SEM with data from four European countries (Finland, the UK, Germany, and Sweden, total N = 3600). The results show that self-focused personal values (Self-enhancement and Openness to change) are not associated with consumers' interest towards meat alternatives. The case is different with other-focused values. Self-transcendence had a positive connection to interest while Conservation had a negative relationship. Finally, the data suggest an underlying role of social motive status between Self-enhancement and interest and the same for group affiliation between Self-transcendence and Conservation and interest. Based on the results, strategies to support meat alternative adoption such as value activation through priming, cognition and emotion-driven marketing are proposed.

The ecological footprint of outdoor activities: Factors affecting human-vectored seed dispersal on clothing.

In the Anthropocene, humans are among the most abundant long-distance seed dispersal vectors globally, due to our increasing mobility and the growing global population. However, there are several knowledge gaps related to the process of human-vectored dispersal (HVD) on clothing. In a multi-site field experiment covering various habitat types in three countries of Central-Europe, we involved 88 volunteer participants and collected 251 HVD samples and 2008 subsamples from their socks and shoes. We analysed the number of diaspores and species in the samples. Specifically, we studied the effects of site characteristics (variables related to habitat types and season), vector characteristics (activity type, gender, clothing type, shoe type) and plant characteristics (species pool of the visited habitats and plant traits) on the number of diaspores and array of species dispersed. We assessed the habits of people that could be relevant for HVD with a questionnaire survey. A total of 35,935 diaspores of 229 plant taxa were identified from the samples, which indicates a huge potential of HVD in dispersing diaspores across habitats and regions. Most diaspores were recorded in grassland habitats, and more diaspores were dispersed during fieldwork than excursions. Clothing type also played a decisive role: there were more diaspores and species when wearing short-top shoes and short trousers than long ones. Even though our study was carried out mainly in natural or semi-natural habitats, a large number of dispersed species were disturbance-tolerants and weeds and only a few were specialists, suggesting the controversial role of HVD in conservation. At the individual level, people can reduce the number of diaspores through their clothing choices and diaspore removal habits, while providing adequate equipment for staff, operating cleaning stations, and increasing awareness of employees are main ways in which unintended diaspore dispersal can be tackled at the institutional level.

Tracking long-term shifts in non-native freshwater macroinvertebrates across three European countries.

Non-native species introductions have been acknowledged as one of the main drivers of freshwater biodiversity decline worldwide, compromising provided ecosystem services and functioning. Despite growing introduction numbers of non-native species, their impacts in conjunction with anthropogenic stressors remain poorly documented. To fill this gap, we studied temporal changes in α (local scale) and γ (regional scale), as well as ß (ratio between γ and α) diversity of non-native freshwater macroinvertebrate species in three European countries (the Netherlands, England and Hungary) using long-term time series data of up to 17 years (2003-2019). We further calculated four ecological and four biological trait metrics to identify changes in trait occurrences over time. We found that α and γ diversities of non-native species were increasing across all countries whereas ß diversity remained stable. We did not identify any significant changes in any trait metric over time, while the predictors tested (land use, climatic predictors, site-specific factor) were similar across countries (e.g., site characteristics or climatic predictors on non-native species trends). Additionally, we projected trends of α, ß, and γ diversity and trait metrics until 2040, which indicated that non-native species will decline across all countries to lower levels except in England for γ diversity and the Netherlands for α diversity where an increase was observed. Thus, our findings indicate shifts in non-native freshwater macroinvertebrate diversity at both local and regional scales in response to the various growing anthropogenic pressures. Our findings underscore the continuous dynamics of non-native species distribution, with the diversity of individual communities and overall landscapes witnessing changes. However, the differentiation in species composition between communities remains unaltered. This could have profound implications for conservation strategies and ecological management in the face of continuously changing biodiversity patterns.

Pathways and drivers of canopy accession across primary temperate forests of Europe.

Canopy accession strategies reveal much about tree life histories and forest stand dynamics. However, the protracted nature of ascending to the canopy makes direct observation challenging. We use a reconstructive approach based on an extensive tree ring database to study the variability of canopy accession patterns of dominant tree species (Abies alba, Acer pseudoplatanus, Fagus sylvatica, Picea abies) in temperate mountain forests of Europe and elucidate how disturbance histories, climate, and topography affect canopy accession. All four species exhibited high variability of radial growth histories leading to canopy accession and indicated varying levels of shade tolerance. Individuals of all four species survived at least 100 years of initial suppression. Fir and particularly beech, however, survived longer periods of initial suppression, exhibited more release events, and reached the canopy later on average, with a larger share of trees accessing the canopy after initially suppressed growth. These results indicate the superior shade tolerance of beech and fir compared to spruce and maple. The two less shade-tolerant species conversely relied on faster growth rates, revealing their competitive advantage in non-suppressed conditions. Additionally, spruce from higher-elevation spruce-dominated forests survived shorter periods of initial shading and exhibited fewer releases, with a larger share of trees reaching the canopy after open canopy recruitment (i.e. in absence of suppression) and no subsequent releases compared to spruce growing in lower-elevation mixed forests. Finally, disturbance factors were identified as the primary driver of canopy accession, whereby disturbances accelerate canopy accession and consequently regulate competitive interactions. Intensifying disturbance regimes could thus promote shifts in species composition, particularly in favour of faster-growing, more light-demanding species.

A Scientometric Overview of the Current Status and Trends of Holistic Nursing Studies.

Holistic nursing care, which has been discussed a lot recently, provides better quality and more effective patient care. This study was planned to analyze the studies conducted on holistic care, by using the bibliometric method and to reveal which types of studies are needed to increase the effectiveness of holistic nursing care and to make it more widespread. The study has the potential to guide scientists who would like to work on this subject. This study describes the current published evidence about holistic nursing between January,1, 1979 and March 18, 2023. The study's data obtained from the Web of Science (WOS) database were analyzed using both quantitative and qualitative measures. The Excel program was used to analyze the study's data, and VOSviewer was used to visualize it. A total of 3887 publications (reviews and articles) obtained in the search for holistic nursing have been published. A total of 2716 (69 875%) of all publications on holistic nursing were published since 2013, and these publications were cited 45 843 times, and 77.832% of all citations were cited to publications published since 2013. The top 5 countries that made the highest number of contributions were the United States (1259 documents), England (457 documents), Australia (380 documents), Canada (253 documents), and Sweden (183 documents). The University of London in England was the top institute focused on holistic nursing. Holistic nursing is a growing and developing subject, especially considering the number of citations and publications in the last 10 years. Most research and cooperation has occurred in a few developed nations: America, Australia, and European countries.

Shifting climatic responses of tree rings and NDVI along environmental gradients.

Variations in the growth of aboveground biomass compartments such as tree stem and foliage significantly influence the carbon cycle of forest ecosystems. Yet the patterns of climate-driven responses of stem and foliage and their modulating factors remain poorly understood. In this study, we investigate the climatic response of Norway spruce (Picea abies) at 138 sites covering wide spatial and site fertility gradients in temperate forests in Central Europe. To characterize the annual growth rate of stem biomass and seasonal canopy vigor, we used tree-ring chronologies and time-series of NDVI derived from Landsat imagery. We calculated correlations of tree-ring width and NDVI with mean growing season temperature and standardized precipitation evapotranspiration index (SPEI). We evaluated how these climate responses varied with aridity index, soil category, stand age, and topographical factors. The results show that the climate-growth responses of tree rings shift from positive to negative for SPEI and from negative to positive for temperature from dry (warm) to wet (cold) areas. By contrast, NDVI revealed a negative response to temperature across the entire climatic gradient. The negative response of NDVI to temperature likely results from drought effects in warm areas and supporting effects of cloudy conditions on foliage greenness in wet areas. Contrary to NDVI, climate responses of tree rings differed according to stand age and were unaffected by local topographical features and soil conditions. Our findings demonstrate that the decoupling of stem and foliage climatic responses may result from their different climatic limitation along environmental gradients. These results imply that in temperate forest ecosystems, the canopy vigor may show different trends compared to stem growth under ongoing climate change.

Supporting Nature-Based Solutions via Nature-Based Thinking across European and Latin American cities.

Nature-Based Solutions concepts and practices are being used worldwide as part of attempts to address societal challenges but have also been criticised for not dealing with deeper transformations needed to face urgent issues including biodiversity loss, climate change and inclusion. In this paper, we explore how an inclusive, integrated and long-sighted approach, emphasising a more radical integration of nature within cities, might support the transformations needed to endure major contemporary challenges. Addressing important emerging critiques of Nature-Based Solutions, we consider the potential of a more incisive form of Nature-Based Thinking (NBT) in cities, based on more holistic perspectives. The paper draws on a reflective and iterative research process that engaged both the research and practice communities through a symposium and a series of futures workshops that together explored the potential of NBT to develop future nature-cities relations in Europe and Latin America. The results of the reflective process suggest that notions of nature with people-not for people- new organisational structures, and the intention and capacity to apply long-term perspectives, are needed when planning for NBS interventions aimed at sustainable urban development. This includes developing a cultural-structural change based on new and inclusive understandings of human-nature relations, and novel governance paradigms that allow cross-sectoral coordination and engagement of local stakeholders beyond formal organisational structures.

Can natural forest expansion contribute to Europe's restoration policy agenda? An interdisciplinary assessment.

Natural forest expansion (NFE), that is, the establishment of secondary forest on non-forested land through natural succession, has substantially contributed to the widespread expansion of forests in Europe over the last few decades. So far, EU policies have largely neglected the potential of NFE for meeting policy objectives on restoration. Synthesising recent interdisciplinary research, this paper assesses the challenges and opportunities of NFE in view of contributing to European forest and ecosystem restoration. Specifically, we discuss the potential for supporting climate change mitigation and adaptation, biodiversity conservation, and forestry and economic use, summarize the current knowledge about societal perceptions and the policymaking on NFE, and make policy recommendations to better use the potential of NFE. We conclude that NFE has the potential to contribute to the European restoration policy agenda if local contexts and possible trade-offs are properly considered.

Climate change adaptation and mitigation strategies for production forests: Trade-offs, synergies, and uncertainties in biodiversity and ecosystem services delivery in Northern Europe.

Climate change adaptation and mitigation strategies (CCAMS) are changes to the management of production forests motivated by the need to mitigate climate change, or adapt production forests to climate change risks. Sweden is employing CCAMS with unclear implications for biodiversity and forest ecosystem services (ES). Here, we synthesized evidence from 51 published scientific reviews, to evaluate the potential implications for biodiversity and a range of provisioning, regulating, and cultural ES, from the adoption of CCAMS relative to standard forestry practice. The CCAMS assessed were the adoption of (i) mixed-species stands, (ii) continuous cover forestry, (iii) altered rotation lengths, (iv) conversion to introduced tree species, (v) logging residue extraction, (vi) stand fertilization, and (vii) altered ditching/draining practices. We highlight the complexity of biodiversity and ES outcomes, identify knowledge gaps, and emphasize the importance of evidence-based decision making and landscape-scale planning when navigating choices involving the widespread adoption of CCAMS.

Social Norms About Dating and Relationship Violence and Gender Among Adolescents: Systematic Review of Measures Used in Dating and Relationship Violence Research.

Adolescent dating and relationship violence (DRV) is widespread and associated with increased risk of subsequent poor mental health outcomes and partner violence. Shifting social norms (i.e., descriptive norms of perceived behavior and injunctive norms of acceptable behavior among a reference group of important others) may be important for reducing DRV. However, few DRV studies assess norms, measurement varies, and evidence on measure quality is diffuse. We aimed to map and assess how studies examining DRV measured social norms concerning DRV and gender. We conducted a systematic review of DRV literature reporting on the use and validity of such measures among participants aged 10-18 years. Searches included English peer-reviewed and grey literature identified via nine databases; Google Scholar; organization websites; reference checking; known studies; and expert requests. We identified 24 eligible studies from the Americas (N = 15), Africa (N = 4), and Europe (N = 5) using 40 eligible measures of DRV norms (descriptive: N = 19; injunctive: N = 14) and gender norms (descriptive: N = 1; injunctive: N = 6). No measure was shared across studies. Most measures were significantly associated with DRV outcomes and most had a defined reference group. Other evidence of quality was mixed. DRV norms measures sometimes specified heterosexual relationships but rarely separated norms governing DRV perpetrated by girls and boys. None specified sexual-minority relationships. Gender norms measures tended to focus on violence, but missed broader gendered expectations underpinning DRV. Future research should develop valid, reliable DRV norms and gender norms measures, and assess whether interventions' impact on norms mediates impact on DRV.

Comparison of Listeria monocytogenes alternative detection methods for food microbiology official controls in Europe.

Listeriosis remains one of the most severe foodborne diseases regarding mortality rate. L. monocytogenes can grow under stressful conditions and contaminate various food categories. Regulation (EC) No 2073/2005 modified on microbiological criteria for foodstuffs includes either qualitative or quantitative food safety criteria on L. monocytogenes and mentions EN ISO 11290-1 Standard as the reference detection method. The use of alternative certified methods is allowed at European level for own checks and by certain European countries in the frame of official controls. Here we report the comparison of alternative certified methods for the detection of L. monocytogenes, according to the criteria expressed by the National Reference Laboratories (NRLs) for L. monocytogenes network in Europe, through an enquiry conducted in 2022 by the European Union Reference Laboratory (EURL) for L. monocytogenes. Emerging detection methods make it possible to diversify the panel of available detection methods. Each method has advantages and limitations and the choice of a method depends on the needs, organization and objectives of the laboratory. Our study focused on a comparison by category in order to provide an overview of the differences between the methods as complete and simple as possible, mainly by examining the certification files of the alternative methods, which constitute a reliable and publicly accessible source of information about performances of the methods.

Reasons for non-adherence with antidepressants using the Medication Adherence Reasons Scale in five European countries and United States.

BACKGROUND: Depression affects approximately 280 million people worldwide. 50 % of individuals with depression discontinue antidepressant therapy within six months of initiation. The study objective was to determine the extent and reasons for non-adherence with antidepressants in the United States (US) and five European Countries (EU). METHODS: Data from the National Health and Wellness Study (NHWS), a self-administered, internet-based cross-sectional survey of US adults in 2019 and 5 EU countries in 2020 was included. NHWS participants who self-reported taking daily prescription medication(s) to treat depression responded to the 19 reasons for non-adherence and one global item in the Medication Adherence Reasons Scale (MAR-Scale). Frequencies were used to identify the reasons for non-adherence. RESULTS: Based on data from 7506 US patients and 4230 EU patients, 46.19 % of patients reported non-adherence to at least one reason in the EU, and 42.9 % in the US. The most common reason for non-adherence in the EU was concern about long-term effects and possible side effects from the medication; and in the US, it was simple forgetfulness. Non-adherence lasted longer due to lack of beliefs in needing medication anymore in both US and EU. LIMITATIONS: The self-reported MAR-Scale can have recall bias and an underestimation of non-adherence. The cross-sectional study design captures the adherence behavior only at one point of time. CONCLUSION: Individuals on antidepressants have to be educated about the need for the medication and their concerns about long term effects and possible side effects from the medication have to be addressed.

Impact of Prostate Cancer in Eastern Europe and Approaches to Treatment and Policy.

Prostate cancer is among the most prevalent cancer globally and within Eastern Europe, where there are also higher levels of mortality compared with Western Europe. Cancer control plans exist in most countries in the region. Attention should be given to devising and implementing optimal screening initiatives. Our review has identified that a lack of resources and health system dysfunctions hamper progress in ameliorating the burden of prostate cancer. Regional cooperation is needed as well as drawing on guidelines and findings from elsewhere. Health institutions must also know the latest developments and set up systems that allow swift adoption.

Representation of women in musculoskeletal radiology conferences in Europe and North and South Americas: an estimation and comparative analysis.

OBJECTIVE: To assess gender distribution and disparities in the musculoskeletal field at radiology conferences and to identify factors associated with the imbalance of women speakers. MATERIALS AND METHODS: This cross-sectional study evaluated publicly available data online from musculoskeletal radiology conference programs from radiological societies across Europe, North America (NA), and South America (SA) from 2016 to 2020. The gender of invited speakers, moderators, and planning committee members and the occurrence of single-gender panels for musculoskeletal and plenary sessions were assessed. RESULTS: A total of 531 sessions, 2580 speakers, 603 moderators, and 231 planning committee members were assessed. Women accounted for 26.6% of total speakers (p<0.001), 33.3% of moderators (p<0.001), and 31.2% of planning committee members (p=0.381). All-male panels were 26.7%, with 21.1% of women moderating these panels (p<0.001). The proportion of women speakers in musculoskeletal and plenary sessions was 29.7% and 34.6% in NA (p=0.035, p=0.052), 26.6% and 25.0% in Europe (p<0.001, p=0.199), and 12.9% and 13.6% in SA (p<0.001). The proportion of women moderators was 35.0% in NA (p=0.002), 37.1% in Europe (p=0.914), and 13.8% in SA (p<0.001). A linear relationship was observed in the proportion of women speakers, moderators, and members of the planning committee (p<0.05). CONCLUSION: We evaluated the participation of women speakers in musculoskeletal radiology conference programs, with significant differences in Europe and South America in all years evaluated, and the participation of women moderators with significant differences in South America and in all-male panels in all regions. Acknowledging gender biases and increasing the number of women on planning committees may help address gender imbalance and promote gender equity.

Platform for Better Oral health in Europe / La plateforme pour la santé orale en Europe

The Platform for Better Oral Health in Europe brings together five European organizations (Council of European Chief Dental Officers, Association for Dental Education in Europe, European Association of Dental Public Health, Pan European-International Association For Dental Research, Oral Health Foundation-UK) along with eighteen other associated European or national organizations. The platform aims to encourage oral health promotion and the prevention of oral diseases as fundamental components of good general health. The aim is thus to strengthen oral health promotion in Europe through integrating oral health into the relevant public health policies. It also aims to address the issue of oral health inequality, particularly among vulnerable populations such as children and adolescents, older adults, and people with particular needs. The platform is therefore a European-level resource for providing evidence-based information on best practice in oral health promotion and for guiding oral health policies. It also works to reinforce communication at the European level between stakeholders, policy makers, health professionals, and the public, in order to improve awareness of oral health issues.

La « plateforme pour une meilleure santé orale en Europe ¼ réunit six associations européennes (Council of European Chief Dental Officers, Association for Dental Education in Europe, European Association of Dental Public Health, Pan European-International Association For Dental Research, Oral Health Fondation-European Federation of Periodontology) en lien avec dix-neuf organisations européennes ou nationales associées. Elle a pour objectif d'encourager la promotion de la santé orale et la prévention des maladies bucco-dentaires en tant qu'éléments fondamentaux d'une bonne santé générale. L'objectif est aussi de renforcer la politique de promotion de la santé orale en Europe, y compris par l'intégration de la santé orale dans des politiques de santé publique pertinentes. Il s'agit également de prendre en compte la question des inégalités en matière de santé orale, notamment au sein des populations vulnérables comme les enfants et les adolescents, les personnes âgées et les personnes ayant des besoins spécifiques. La plateforme constitue ainsi une ressource au niveau européen pour fournir des informations fondées sur des preuves concernant les meilleures pratiques en promotion de la santé orale et pour l'orientation des politiques en matière de santé orale. Elle travaille aussi à développer les relations au niveau européen avec les parties prenantes, les décideurs politiques, les professionnels de santé, le public, afin d'améliorer la prise en compte des enjeux concernant la santé orale en Europe.

Introduction of invasive mosquito species into Europe and prospects for arbovirus transmission and vector control in an era of globalization.

BACKGROUND: Mosquito research in Europe has a long history, primarily focused on malaria vectors. In recent years, invasive mosquito species like the Asian tiger mosquito (Aedes albopictus) and the spread of arboviruses like dengue virus, chikungunya virus or bluetongue virus have led to an intensification of research and monitoring in Europe. The risk of further dissemination of exotic species and mosquito-borne pathogens is expected to increase with ongoing globalization, human mobility, transport geography, and climate warming. Researchers have conducted various studies to understand the ecology, biology, and effective control strategies of mosquitoes and associated pathogens. MAIN BODY: Three invasive mosquito species are established in Europe: Asian tiger mosquito (Aedes albopictus), Japanese bush mosquito (Ae. japonicus), and Korean bush mosquito (Aedes koreicus). Ae. albopictus is the most invasive species and has been established in Europe since 1990. Over the past two decades, there has been an increasing number of outbreaks of infections by mosquito-borne viruses in particular chikungunya virus, dengue virus or Zika virus in Europe primary driven by Ae. albopictus. At the same time, climate change with rising temperatures results in increasing threat of invasive mosquito-borne viruses, in particular Usutu virus and West Nile virus transmitted by native Culex mosquito species. Effective mosquito control programs require a high level of community participation, going along with comprehensive information campaigns, to ensure source reduction and successful control. Control strategies for container breeding mosquitoes like Ae. albopictus or Culex species involve community participation, door-to-door control activities in private areas. Further measures can involve integration of sterile insect techniques, applying indigenous copepods, Wolbachia sp. bacteria, or genetically modified mosquitoes, which is very unlike to be practiced as standard method in the near future. CONCLUSIONS: Climate change and globalization resulting in the increased establishment of invasive mosquitoes in particular of the Asian tiger mosquito Ae. albopictus in Europe within the last 30 years and increasing outbreaks of infections by mosquito-borne viruses warrants intensification of research and monitoring. Further, effective future mosquito control programs require increase in intense community and private participation, applying physical, chemical, biological, and genetical control activities.

HIV diagnoses among people born in Ukraine reported by EU/EEA countries in 2022: impact on regional HIV trends and implications for healthcare planning.

Following Russia's invasion in 2022, over 4.1 million Ukrainians sought refuge in the EU/EEA. We assessed how this impacted HIV case reporting by EU/EEA countries. Ukrainian refugees constituted 10.2% (n = 2,338) of all 2022 HIV diagnoses, a 10-fold increase from 2021. Of these, 9.3% (n = 217) were new diagnoses, 58.5% (n = 1,368) were previously identified; 32.2% had unknown status. Displacement of Ukrainians has partly contributed to increasing HIV diagnoses in EU/EEA countries in 2022, highlighting the importance of prevention, testing and care.

Ecological and clinical evidence of the establishment of West Nile virus in a large urban area in Europe, Berlin, Germany, 2021 to 2022.

BackgroundWest Nile virus (WNV), found in Berlin in birds since 2018 and humans since 2019, is a mosquito-borne virus that can manifest in humans as West Nile fever (WNF) or neuroinvasive disease (WNND). However, human WNV infections and associated disease are likely underdiagnosed.AimWe aimed to identify and genetically characterise WNV infections in humans and mosquitoes in Berlin.MethodsWe investigated acute WNV infection cases reported to the State Office for Health and Social Affairs Berlin in 2021 and analysed cerebrospinal fluid (CSF) samples from patients with encephalitis of unknown aetiology (n = 489) for the presence of WNV. Mosquitoes were trapped at identified potential exposure sites of cases and examined for WNV infection.ResultsWest Nile virus was isolated and sequenced from a blood donor with WNF, a symptomatic patient with WNND and a WNND case retrospectively identified from testing CSF. All cases occurred in 2021 and had no history of travel 14 days prior to symptom onset (incubation period of the disease). We detected WNV in Culex pipiens mosquitoes sampled at the exposure site of one case in 2021, and in 2022. Genome analyses revealed a monophyletic Berlin-specific virus clade in which two enzootic mosquito-associated variants can be delineated based on tree topology and presence of single nucleotide variants. Both variants have highly identical counterparts in human cases indicating local acquisition of infection.ConclusionOur study provides evidence that autochthonous WNV lineage 2 infections occurred in Berlin and the virus has established an endemic maintenance cycle.

Collaborative study for the establishment of replacement batches of Ph. Eur. Heparin Low-Molecular-Mass for Calibration CRS.

An international collaborative study was run within the framework of the Biological Standardisation Programme (BSP) of the Council of Europe and the Commission of the European Union to establish replacement batches for European Pharmacopoeia (Ph. Eur.) Heparin Low-Molecular-Mass (LMM) for calibration Chemical Reference Substance batch 3 (CRS3) used for the characterisation of LMM heparins by high performance size-exclusion chromatography. Two candidate batches (A, cCRS4 and B, cCRS5) were filled using the same material as the existing official calibrants, adopted with either an assigned number-average molecular mass (Mna) or a broad standard table (BST). Fifteen laboratories evaluated the suitability of these candidate batches for use as calibrants with the pharmacopoeial dual refractive index/ultraviolet (RI/UV) detector calibration method, as well as with a modified mobile phase and the BST calibration method. Seven preparations of LMM heparin were tested. The results confirmed that the proposed batches are suitable for use with the same characteristic Mna as CRS3 and with the BST established for the World Health Organization (WHO) 2nd International Standard (IS). The BST calibration method gave comparable results to the RI/UV method, while showing better reproducibility, being easier to perform and requiring no calibrant with UV absorbance. The modified mobile phase had no impact on the calculated values while improving separation between the calibrant and salt peaks. The two candidate batches were adopted as Ph. Eur. Heparin LMM for calibration CRS batches 4 and 5, respectively, with the assigned Mna value of 3800 and a BST. In anticipation of the depletion of the calibrant required for use with the RI/UV method, and taking into account the unlikely procurement of a new lot of suitable starting material, it was recommended to include the BST method in Ph. Eur. monograph 0828, Heparins, low-molecular-mass. In order to improve peak separation, it was also recommended to include the use of ammonium acetate solution as mobile phase in the monograph, both for the Ph. Eur. RI/UV and the proposed BST calibration methods. Further to this study, Ph. Eur. monograph 0828 was revised to replace the RI/UV method by the BST method. This contributed to the harmonisation of methods across regions, thereby facilitating a concerted global action for the development and establishment of the next batches of calibrants for the quality control of LMM heparins.

19th Century Deciduous Tooth Boxes from France and Belgium.

An encounter 50 years ago led to an interesting collection of tooth boxes. This paper will highlight many of those unique treasures emanating from Northwestern Europe.