Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 73.651
Filtrar
1.
Planta ; 253(4): 86, 2021 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-33792791

RESUMEN

MAIN CONCLUSIONS: While two lineages of retrotransposons were more abundant in larger Passiflora genomes, the satellitome was more diverse and abundant in the smallest genome analysed. Repetitive sequences are ubiquitous and fast-evolving elements responsible for size variation and large-scale organization of plant genomes. Within Passiflora genus, a tenfold variation in genome size, not attributed to polyploidy, is known. Here, we applied a combined in silico and cytological approach to study the organization and diversification of repetitive elements in three species of this genus representing its known range in genome size variation. Sequences were classified in terms of type and repetitiveness and the most abundant were mapped to chromosomes. We identified long terminal repeat (LTR) retrotransposons as the most abundant elements in the three genomes, showing a considerable variation among species. Satellite DNAs (satDNAs) were less representative, but highly diverse between subgenera. Our results clearly confirm that the largest genome species (Passiflora quadrangularis) presents a higher accumulation of repetitive DNA sequences, specially Angela and Tekay elements, making up most of its genome. Passiflora cincinnata, with intermediate genome and from the same subgenus, showed similarity with P. quadrangularis regarding the families of repetitive DNA sequences, but in different proportions. On the other hand, Passiflora organensis, the smallest genome, from a different subgenus, presented greater diversity and the highest proportion of satDNA. Altogether, our data indicates that while large genomes evolved by an accumulation of retrotransposons, the smallest genome known for the genus has evolved by diversification of different repeat types, particularly satDNAs.


Asunto(s)
ADN Satélite/genética , Genoma de Planta , Passiflora/genética , Retroelementos , Evolución Molecular , Variación Genética , Filogenia
2.
Science ; 371(6535): 1225-1232, 2021 Mar 19.
Artículo en Inglés | MEDLINE | ID: mdl-33737482

RESUMEN

Early life is thought to have required the self-replication of RNA by RNA replicases. However, how such replicases evolved and subsequently enabled gene expression remains largely unexplored. We engineered and selected a holopolymerase ribozyme that uses a sigma factor-like specificity primer to first recognize an RNA promoter sequence and then, in a second step, rearrange to a processive elongation form. Using its own sequence, the polymerase can also program itself to polymerize from certain RNA promoters and not others. This selective promoter-based polymerization could allow an RNA replicase ribozyme to define "self" from "nonself," an important development for the avoidance of replicative parasites. Moreover, the clamp-like mechanism of this polymerase could eventually enable strand invasion, a critical requirement for replication in the early evolution of life.


Asunto(s)
Regiones Promotoras Genéticas , ARN Catalítico , ARN/química , ARN/metabolismo , Evolución Molecular Dirigida , Evolución Molecular , Mutación , Conformación de Ácido Nucleico , Polimerizacion , Dominios Proteicos , ARN/genética , ARN Catalítico/química , ARN Catalítico/genética , ARN Catalítico/metabolismo , /genética , Factor sigma/metabolismo
3.
Nat Commun ; 12(1): 1396, 2021 03 02.
Artículo en Inglés | MEDLINE | ID: mdl-33654096

RESUMEN

Increasing numbers of protein interactions have been identified in high-throughput experiments, but only a small proportion have solved structures. Recently, sequence coevolution-based approaches have led to a breakthrough in predicting monomer protein structures and protein interaction interfaces. Here, we address the challenges of large-scale interaction prediction at residue resolution with a fast alignment concatenation method and a probabilistic score for the interaction of residues. Importantly, this method (EVcomplex2) is able to assess the likelihood of a protein interaction, as we show here applied to large-scale experimental datasets where the pairwise interactions are unknown. We predict 504 interactions de novo in the E. coli membrane proteome, including 243 that are newly discovered. While EVcomplex2 does not require available structures, coevolving residue pairs can be used to produce structural models of protein interactions, as done here for membrane complexes including the Flagellar Hook-Filament Junction and the Tol/Pal complex.


Asunto(s)
Aminoácidos/genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Evolución Molecular , Genoma Bacteriano , Mapeo de Interacción de Proteínas , Proteínas Bacterianas/química , Secuencia de Bases , Escherichia coli/genética , Células Eucariotas/metabolismo , Proteínas de la Membrana/metabolismo , Simulación del Acoplamiento Molecular , Unión Proteica , Proteoma/metabolismo
4.
Nat Commun ; 12(1): 1447, 2021 03 04.
Artículo en Inglés | MEDLINE | ID: mdl-33664263

RESUMEN

Identifying the genetic factors that underlie complex traits is central to understanding the mechanistic underpinnings of evolution. Cave-dwelling Astyanax mexicanus populations are well adapted to subterranean life and many populations appear to have evolved troglomorphic traits independently, while the surface-dwelling populations can be used as a proxy for the ancestral form. Here we present a high-resolution, chromosome-level surface fish genome, enabling the first genome-wide comparison between surface fish and cavefish populations. Using this resource, we performed quantitative trait locus (QTL) mapping analyses and found new candidate genes for eye loss such as dusp26. We used CRISPR gene editing in A. mexicanus to confirm the essential role of a gene within an eye size QTL, rx3, in eye formation. We also generated the first genome-wide evaluation of deletion variability across cavefish populations to gain insight into this potential source of cave adaptation. The surface fish genome reference now provides a more complete resource for comparative, functional and genetic studies of drastic trait differences within a species.


Asunto(s)
Adaptación Fisiológica/genética , Characidae/embriología , Characidae/genética , Ojo/embriología , Herencia Multifactorial/genética , Animales , Evolución Biológica , Cuevas , Mapeo Cromosómico , Evolución Molecular , Edición Génica , Genoma/genética , Proteínas de Homeodominio/genética , Fosfatasas de la Proteína Quinasa Activada por Mitógenos/genética , Sitios de Carácter Cuantitativo/genética
5.
Hawaii J Health Soc Welf ; 80(3): 52-61, 2021 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-33718878

RESUMEN

The COVID-19 pandemic has ravaged the world, caused over 1.8 million deaths in its first year, and severely affected the global economy. Hawai'i has not been spared from the transmission of SARS-CoV-2 in the local population, including high infection rates in racial and ethnic minorities. Early in the pandemic, we described in this journal various technologies used for the detection of SARS-CoV-2. Herein we characterize a 969-bp SARS-CoV-2 segment of the S gene downstream of the receptor-binding domain. At the John A. Burns School of Medicine Biocontainment Facility, RNA was extracted from an oropharyngeal swab and a nasal swab from 2 patients from Hawai'i who were infected with SARS-CoV-2 in August 2020. Following PCR, the 2 viral strains were sequenced using Sanger sequencing, and phylogenetic trees were generated using MEGAX. Phylogenetic tree results indicate that the virus has been introduced to Hawai'i from multiple sources. Further, we decoded 13 single nucleotide polymorphisms across 13 unique SARS-CoV-2 genomes within this region of the S gene, with 1 non-synonymous mutation (P681H) found in the 2 Hawai'i strains. The P681H mutation has unique and emerging characteristics with a significant exponential increase in worldwide frequency when compared to the plateauing of the now universal D614G mutation. The P681H mutation is also characteristic of the new SARS-CoV-2 variants from the United Kingdom and Nigeria. Additionally, several mutations resulting in cysteine residues were detected, potentially resulting in disruption of the disulfide bridges in and around the receptor-binding domain. Targeted sequence characterization is warranted to determine the origin of multiple introductions of SARS-CoV-2 circulating in Hawai'i.


Asunto(s)
/virología , Genoma Viral/genética , Mutación/genética , /genética , Evolución Molecular , Hawaii/epidemiología , Humanos , Filogenia , Análisis de Secuencia
6.
Nat Commun ; 12(1): 1607, 2021 03 11.
Artículo en Inglés | MEDLINE | ID: mdl-33707453

RESUMEN

In recognizing the host cellular receptor and mediating fusion of virus and cell membranes, the spike (S) glycoprotein of coronaviruses is the most critical viral protein for cross-species transmission and infection. Here we determined the cryo-EM structures of the spikes from bat (RaTG13) and pangolin (PCoV_GX) coronaviruses, which are closely related to SARS-CoV-2. All three receptor-binding domains (RBDs) of these two spike trimers are in the "down" conformation, indicating they are more prone to adopt the receptor-binding inactive state. However, we found that the PCoV_GX, but not the RaTG13, spike is comparable to the SARS-CoV-2 spike in binding the human ACE2 receptor and supporting pseudovirus cell entry. We further identified critical residues in the RBD underlying different activities of the RaTG13 and PCoV_GX/SARS-CoV-2 spikes. These results collectively indicate that tight RBD-ACE2 binding and efficient RBD conformational sampling are required for the evolution of SARS-CoV-2 to gain highly efficient infection.


Asunto(s)
/virología , Quirópteros/virología , Coronavirus/química , Coronavirus/genética , /química , Glicoproteína de la Espiga del Coronavirus/química , Glicoproteína de la Espiga del Coronavirus/genética , Secuencia de Aminoácidos , Animales , /transmisión , Microscopía por Crioelectrón , Evolución Molecular , Interacciones Microbiota-Huesped , Humanos , Modelos Moleculares , Pandemias , Dominios Proteicos , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Glicoproteína de la Espiga del Coronavirus/ultraestructura
7.
Nat Commun ; 12(1): 1361, 2021 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-33649327

RESUMEN

Sperm contributes diverse RNAs to the zygote. While sperm small RNAs have been shown to impact offspring phenotypes, our knowledge of the sperm transcriptome, especially the composition of long RNAs, has been limited by the lack of sensitive, high-throughput experimental techniques that can distinguish intact RNAs from fragmented RNAs, known to abound in sperm. Here, we integrate single-molecule long-read sequencing with short-read sequencing to detect sperm intact RNAs (spiRNAs). We identify 3440 spiRNA species in mice and 4100 in humans. The spiRNA profile consists of both mRNAs and long non-coding RNAs, is evolutionarily conserved between mice and humans, and displays an enrichment in mRNAs encoding for ribosome. In sum, we characterize the landscape of intact long RNAs in sperm, paving the way for future studies on their biogenesis and functions. Our experimental and bioinformatics approaches can be applied to other tissues and organisms to detect intact transcripts.


Asunto(s)
Secuencia Conservada/genética , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , ARN/genética , Imagen Individual de Molécula , Espermatozoides/metabolismo , Animales , Evolución Molecular , Ontología de Genes , Humanos , Masculino , Ratones Endogámicos C57BL , ARN/metabolismo , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ribosomas/metabolismo , Testículo/metabolismo , Transcriptoma/genética
8.
Yi Chuan ; 43(3): 215-225, 2021 Mar 16.
Artículo en Inglés | MEDLINE | ID: mdl-33724206

RESUMEN

Transcription factors are a class of proteins that regulate gene transcription and expression by binding to gene-specific sequences and play an essential role in regulating the biological activities of cells. The RHR (Rel-homology region) transcription factor family is the primary member of the IF (immunoglobulin fold) transcription factor superfamily, whose members contain the conserved Rel domain and IPT (immunoglobulin-like fold) domain. As an ancient transcription factor family, the RHR family continues differentiation on gene gain and loss through gene duplication, mutation, and silencing, accompanied with the evolution of diverse species. Natural selection has led to different rates of evolution among members of the family, and some domains of the protein family have shown unique mechanisms of evolution. However, the current reviews about the origin and differentiation of RHR family are rare. In this review, we summarize the research results on the distribution, classification, function, and evolution of the members of the RHR family in order to provide a reference and new idea for studying the evolution mechanism of the whole transcription factor family and the evolutionary relationship among species.


Asunto(s)
Regulación de la Expresión Génica , Factores de Transcripción , Evolución Molecular , Familia de Multigenes , Filogenia , Selección Genética , Factores de Transcripción/genética
10.
PLoS One ; 16(3): e0247799, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33662015

RESUMEN

COVID-19 has greatly affected public health and world economy. In this study, we analyzed 129 full-length genomes of SARS-CoV-2 viruses of a Canadian population during early phase of the pandemic. Phylogenetic analysis revealed three major paths of transmission of SARS-CoV-2 viruses into Canada. Twenty-one substitutions that have frequencies greater than 3% of viral population were identified. Analysis of these substitutions indicated that P1427I (ORF1b), Y1464C (ORF1b), and Q57H (ORF3a) might affect functions of the corresponding SARS-CoV-2 encoded proteins. Additionally, we found the evidence of positive selection on the ORF3a and codon 614 of Spike protein, suggesting the viral components responsible for host entry and activation of inflammation response were targeted by host immune responses. The study showed genomic variation and evolution of SARS-CoV-2 in a Canadian population. These information may help develop preventive strategies and be used for further study of SARS-CoV-2 pathogenesis and therapeutics development.


Asunto(s)
/virología , Genoma Viral , /genética , /epidemiología , Canadá/epidemiología , Evolución Molecular , Variación Genética , Humanos , Filogenia , Glicoproteína de la Espiga del Coronavirus/genética , Proteínas Virales/genética
11.
Nat Commun ; 12(1): 1422, 2021 03 03.
Artículo en Inglés | MEDLINE | ID: mdl-33658492

RESUMEN

Trans-acyltransferase polyketide synthases (trans-AT PKSs) are bacterial multimodular enzymes that biosynthesize diverse pharmaceutically and ecologically important polyketides. A notable feature of this natural product class is the existence of chemical hybrids that combine core moieties from different polyketide structures. To understand the prevalence, biosynthetic basis, and evolutionary patterns of this phenomenon, we developed transPACT, a phylogenomic algorithm to automate global classification of trans-AT PKS modules across bacteria and applied it to 1782 trans-AT PKS gene clusters. These analyses reveal widespread exchange patterns suggesting recombination of extended PKS module series as an important mechanism for metabolic diversification in this natural product class. For three plant-associated bacteria, i.e., the root colonizer Gynuella sunshinyii and the pathogens Xanthomonas cannabis and Pseudomonas syringae, we demonstrate the utility of this computational approach for uncovering cryptic relationships between polyketides, accelerating polyketide mining from fragmented genome sequences, and discovering polyketide variants with conserved moieties of interest. As natural combinatorial hybrids are rare among the more commonly studied cis-AT PKSs, this study paves the way towards evolutionarily informed, rational PKS engineering to produce chimeric trans-AT PKS-derived polyketides.


Asunto(s)
Aciltransferasas/genética , Proteínas Bacterianas/genética , Filogenia , Sintasas Poliquetidas/genética , Policétidos/metabolismo , Aciltransferasas/metabolismo , Algoritmos , Arabidopsis/microbiología , Proteínas Bacterianas/metabolismo , Evolución Molecular , Genoma Bacteriano , Células HeLa , Humanos , Lactonas/metabolismo , Macrólidos/metabolismo , Familia de Multigenes , Piperidonas/química , Plantas/microbiología , Sintasas Poliquetidas/metabolismo , Policétidos/química , Pseudomonas syringae/metabolismo , Xanthomonas/metabolismo , Xanthomonas/patogenicidad
12.
PLoS Biol ; 19(3): e3001115, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33711012

RESUMEN

Virus host shifts are generally associated with novel adaptations to exploit the cells of the new host species optimally. Surprisingly, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has apparently required little to no significant adaptation to humans since the start of the Coronavirus Disease 2019 (COVID-19) pandemic and to October 2020. Here we assess the types of natural selection taking place in Sarbecoviruses in horseshoe bats versus the early SARS-CoV-2 evolution in humans. While there is moderate evidence of diversifying positive selection in SARS-CoV-2 in humans, it is limited to the early phase of the pandemic, and purifying selection is much weaker in SARS-CoV-2 than in related bat Sarbecoviruses. In contrast, our analysis detects evidence for significant positive episodic diversifying selection acting at the base of the bat virus lineage SARS-CoV-2 emerged from, accompanied by an adaptive depletion in CpG composition presumed to be linked to the action of antiviral mechanisms in these ancestral bat hosts. The closest bat virus to SARS-CoV-2, RmYN02 (sharing an ancestor about 1976), is a recombinant with a structure that includes differential CpG content in Spike; clear evidence of coinfection and evolution in bats without involvement of other species. While an undiscovered "facilitating" intermediate species cannot be discounted, collectively, our results support the progenitor of SARS-CoV-2 being capable of efficient human-human transmission as a consequence of its adaptive evolutionary history in bats, not humans, which created a relatively generalist virus.


Asunto(s)
/virología , Quirópteros/virología , /virología , Animales , /transmisión , Evolución Molecular , Genoma Viral , Especificidad del Huésped , Humanos , Pandemias , Filogenia , Receptores Virales/genética , Selección Genética , /transmisión
14.
Science ; 371(6533)2021 03 05.
Artículo en Inglés | MEDLINE | ID: mdl-33674467

RESUMEN

The mechanisms that underly the adaptation of enzyme activities and stabilities to temperature are fundamental to our understanding of molecular evolution and how enzymes work. Here, we investigate the molecular and evolutionary mechanisms of enzyme temperature adaption, combining deep mechanistic studies with comprehensive sequence analyses of thousands of enzymes. We show that temperature adaptation in ketosteroid isomerase (KSI) arises primarily from one residue change with limited, local epistasis, and we establish the underlying physical mechanisms. This residue change occurs in diverse KSI backgrounds, suggesting parallel adaptation to temperature. We identify residues associated with organismal growth temperature across 1005 diverse bacterial enzyme families, suggesting widespread parallel adaptation to temperature. We assess the residue properties, molecular interactions, and interaction networks that appear to underly temperature adaptation.


Asunto(s)
Adaptación Fisiológica , Proteínas Bacterianas/química , Evolución Molecular , Esteroide Isomerasas/química , Sustitución de Aminoácidos , Proteínas Bacterianas/genética , Estabilidad de Enzimas , Mutación , Esteroide Isomerasas/genética , Temperatura
16.
Nat Commun ; 12(1): 1852, 2021 03 25.
Artículo en Inglés | MEDLINE | ID: mdl-33767175

RESUMEN

TEM-1 ß-lactamase degrades ß-lactam antibiotics with a strong preference for penicillins. Sequence reconstruction studies indicate that it evolved from ancestral enzymes that degraded a variety of ß-lactam antibiotics with moderate efficiency. This generalist to specialist conversion involved more than 100 mutational changes, but conserved fold and catalytic residues, suggesting a role for dynamics in enzyme evolution. Here, we develop a conformational dynamics computational approach to rationally mold a protein flexibility profile on the basis of a hinge-shift mechanism. By deliberately weighting and altering the conformational dynamics of a putative Precambrian ß-lactamase, we engineer enzyme specificity that mimics the modern TEM-1 ß-lactamase with only 21 amino acid replacements. Our conformational dynamics design thus re-enacts the evolutionary process and provides a rational allosteric approach for manipulating function while conserving the enzyme active site.


Asunto(s)
beta-Lactamasas/genética , beta-Lactamasas/metabolismo , Secuencia de Aminoácidos/genética , Dominio Catalítico/genética , Biología Computacional , Escherichia coli/efectos de los fármacos , Escherichia coli/enzimología , Evolución Molecular , Simulación de Dinámica Molecular , Penicilinas/metabolismo , Conformación Proteica , Especificidad por Sustrato
17.
Nat Commun ; 12(1): 1879, 2021 03 25.
Artículo en Inglés | MEDLINE | ID: mdl-33767194

RESUMEN

In modern oceans, eukaryotic phytoplankton is dominated by lineages with red algal-derived plastids such as diatoms, dinoflagellates, and coccolithophores. Despite the ecological importance of these groups and many others representing a huge diversity of forms and lifestyles, we still lack a comprehensive understanding of their evolution and how they obtained their plastids. New hypotheses have emerged to explain the acquisition of red algal-derived plastids by serial endosymbiosis, but the chronology of these putative independent plastid acquisitions remains untested. Here, we establish a timeframe for the origin of red algal-derived plastids under scenarios of serial endosymbiosis, using Bayesian molecular clock analyses applied on a phylogenomic dataset with broad sampling of eukaryote diversity. We find that the hypotheses of serial endosymbiosis are chronologically possible, as the stem lineages of all red plastid-containing groups overlap in time. This period in the Meso- and Neoproterozoic Eras set the stage for the later expansion to dominance of red algal-derived primary production in the contemporary oceans, which profoundly altered the global geochemical and ecological conditions of the Earth.


Asunto(s)
Evolución Molecular , Plastidios/genética , Rhodophyta/genética , Evolución Biológica , Diatomeas/genética , Dinoflagelados/genética , Haptophyta/genética , Océanos y Mares , Fotosíntesis/genética , Fotosíntesis/fisiología , Plastidios/metabolismo , Simbiosis/genética
18.
Biochem Biophys Res Commun ; 550: 8-14, 2021 04 23.
Artículo en Inglés | MEDLINE | ID: mdl-33676232

RESUMEN

The SARS-CoV-2 Variant of Concern 202012/01 (VOC-202012/01) emerged in southeast England and rapidly spread worldwide. This variant is believed to be more transmissible, with all attention being given to its spike mutations. However, VOC-202012/01 has also a mutation (Q27stop) that truncates the ORF8, a likely immune evasion protein. Removal of ORF8 changes the clinical outset of the disease, which may affect the virus transmissibility. Here I provide a detailed analysis of all reported ORF8-deficient lineages found in the background of relevant spike mutations, identified among 231,433 SARS-CoV-2 genomes. I found 19 ORF8 nonsense mutations, most of them occurring in the 5' half of the gene. The ORF8-deficient lineages were rare, representing 0.67% of sequenced genomes. Nevertheless, I identified two clusters of related sequences that emerged recently and spread in different countries. The widespread D614G spike mutation was found in most ORF-deficient lineages. Although less frequent, HV69-70del and L5F spike mutations occurred in the background of six different ORF8 nonsense mutations. I also confirmed that VOC-202012/01 is the ORF8-deficient variant with more spike mutations reported to date, although other variants could have up to six spike mutations, some of putative biological relevance. Overall, these results suggest that monitoring ORF8-deficient lineages is important for the progression of the COVID-19 pandemic, particularly when associated with relevant spike mutations.


Asunto(s)
/transmisión , Monitoreo Epidemiológico , Eliminación de Gen , Glicoproteína de la Espiga del Coronavirus/genética , Proteínas Virales/genética , /epidemiología , Codón sin Sentido , Codón de Terminación/genética , Evolución Molecular , Genes Virales/genética , Humanos , Filogenia , Selección Genética , Factores de Tiempo , Reino Unido/epidemiología
19.
BMC Microbiol ; 21(1): 89, 2021 03 23.
Artículo en Inglés | MEDLINE | ID: mdl-33757449

RESUMEN

BACKGROUND: When a virus that has grown in a nonhuman host starts an epidemic in the human population, human cells may not provide growth conditions ideal for the virus. Therefore, the invasion of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), which is usually prevalent in the bat population, into the human population is thought to have necessitated changes in the viral genome for efficient growth in the new environment. In the present study, to understand host-dependent changes in coronavirus genomes, we focused on the mono- and oligonucleotide compositions of SARS-CoV-2 genomes and investigated how these compositions changed time-dependently in the human cellular environment. We also compared the oligonucleotide compositions of SARS-CoV-2 and other coronaviruses prevalent in humans or bats to investigate the causes of changes in the host environment. RESULTS: Time-series analyses of changes in the nucleotide compositions of SARS-CoV-2 genomes revealed a group of mono- and oligonucleotides whose compositions changed in a common direction for all clades, even though viruses belonging to different clades should evolve independently. Interestingly, the compositions of these oligonucleotides changed towards those of coronaviruses that have been prevalent in humans for a long period and away from those of bat coronaviruses. CONCLUSIONS: Clade-independent, time-dependent changes are thought to have biological significance and should relate to viral adaptation to a new host environment, providing important clues for understanding viral host adaptation mechanisms.


Asunto(s)
Composición de Base , Evolución Molecular , Genoma Viral , /genética , Animales , Quirópteros/virología , Humanos , Oligonucleótidos
20.
Nat Commun ; 12(1): 1667, 2021 03 12.
Artículo en Inglés | MEDLINE | ID: mdl-33712569

RESUMEN

Comparative genomic studies have repeatedly shown that new protein-coding genes can emerge de novo from noncoding DNA. Still unknown is how and when the structures of encoded de novo proteins emerge and evolve. Combining biochemical, genetic and evolutionary analyses, we elucidate the function and structure of goddard, a gene which appears to have evolved de novo at least 50 million years ago within the Drosophila genus. Previous studies found that goddard is required for male fertility. Here, we show that Goddard protein localizes to elongating sperm axonemes and that in its absence, elongated spermatids fail to undergo individualization. Combining modelling, NMR and circular dichroism (CD) data, we show that Goddard protein contains a large central α-helix, but is otherwise partially disordered. We find similar results for Goddard's orthologs from divergent fly species and their reconstructed ancestral sequences. Accordingly, Goddard's structure appears to have been maintained with only minor changes over millions of years.


Asunto(s)
Drosophila/genética , Evolución Molecular , Animales , Fertilidad/genética , Regulación del Desarrollo de la Expresión Génica , Genómica , Masculino , Simulación de Dinámica Molecular , Proteínas/metabolismo , Espermátides , Espermatozoides , Transgenes
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...