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1.
Tumour Biol ; 43(1): 37-55, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33935122

RESUMEN

BACKGROUND: Green synthesized nanoparticles have been earmarked for use in nanomedicine including for the development of better anticancer drugs. OBJECTIVE: The aim of this study was to undertake biochemical evaluation of anticancer activities of green synthesized silver nanoparticles (AgNPs) from ethanolic extracts of fruits (AgNPs-F) and leaves (AgNPs-L) of Annona muricata. METHODS: Previously synthesized silver nanoparticles were used for the study. The effects of the AgNPs and 5-Fluorouracil were studied on PC3, HeLa and PNT1A cells. The resazurin, migration and colonogenic assays as well as qRT-PCR were employed. RESULTS: The AgNPs-F displayed significant antiproliferative effects against HeLa cells with an IC50 of 38.58µg/ml and PC3 cells with an IC50 of 48.17µg/ml but selectively spared normal PNT1A cells (selectivity index of 7.8), in comparison with first line drug 5FU and AgNPs-L whose selectivity index were 3.56 and 2.26 respectively. The migration assay revealed potential inhibition of the metastatic activity of the cells by the AgNPs-F while the colonogenic assay indicated the permanent effect of the AgNPs-F on the cancer cells yet being reversible on the normal cells in contrast with 5FU and AgNPs-L. CASP9 was significantly over expressed in all HeLa cells treated with the AgNPs-F (1.53-fold), AgNPs-L (1.52-fold) and 5FU (4.30-fold). CXCL1 was under expressed in HeLa cells treated with AgNPs-F (0.69-fold) and AgNPs-L (0.58-fold) and over expressed in cells treated with 5FU (4.95-fold), but the difference was not statistically significant. CXCR2 was significantly over expressed in HeLa cells treated with 5FU (8.66-fold) and AgNPs-F (1.12-fold) but under expressed in cells treated with AgNPs-L (0.76-fold). CONCLUSIONS: Here we show that biosynthesized AgNPs especially AgNPs-F can be used in the development of novel and better anticancer drugs. The mechanism of action of the AgNPs involves activation of the intrinsic apoptosis pathway through upregulation of CASP9 and concerted down regulation of the CXCL1/ CXCR2 gene axis.


Asunto(s)
Annona/química , Antineoplásicos/farmacología , Caspasa 9/genética , Quimiocina CXCL1/genética , Nanopartículas del Metal , Receptores de Interleucina-8B/genética , Plata/farmacología , Adenocarcinoma/patología , Línea Celular , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Femenino , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Tecnología Química Verde , Humanos , Masculino , Extractos Vegetales/química , Extractos Vegetales/farmacología , Neoplasias de la Próstata/patología , Neoplasias del Cuello Uterino/patología
2.
Nat Prod Res ; 35(9): 1544-1549, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33938336

RESUMEN

One new secoiridoid compound swertiamarin B (1), along with a known compound lytanthosalin (2), were isolated from ethanol extract of the aerial parts of Swertia mussotii. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. All compounds were first isolated from the Swertia genus. Their antitumor activities were evaluated for four human tumor cell lines (HCT-116, HepG2, MGC-803 and A549). Compounds 1 and 2 showed excellent cytotoxic activities toward the MGC-803 cell lines with IC50 values 3.61 and 12.04 µM, respectively.


Asunto(s)
Iridoides/aislamiento & purificación , Iridoides/farmacología , Componentes Aéreos de las Plantas/química , Swertia/química , Espectroscopía de Resonancia Magnética con Carbono-13 , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Humanos , Concentración 50 Inhibidora , Glucósidos Iridoides/química , Glucósidos Iridoides/aislamiento & purificación , Glucósidos Iridoides/farmacología , Iridoides/química , Extractos Vegetales/química , Espectroscopía de Protones por Resonancia Magnética , Pironas/química , Pironas/aislamiento & purificación , Pironas/farmacología
3.
Georgian Med News ; (312): 138-145, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33964842

RESUMEN

The article presents the results of biochemical studies of blood and morphological characteristics of liver changes in laboratory animals (rats) under experimental conditions of paracetamol hepatitis and intragastric administration of a new substance G10 from the «Zhuzgun¼ plant in various doses. The obtained data open up prospects for further studies of the pharmacological properties of the substance G10, the possibility of including it as a phytotherapeutic agent in the complex of preventive and therapeutic measures for acute toxic hepatitis. The study of the hepatoprotective properties was conducted in the "Educational and Research Pharmacological Laboratory" of the Department of General Pharmacology of the Astana Medical University. The object of the study was the substance G. 10 from the Juzgun plant, which is a brown powder, odorless, poorly soluble in water. Тhe analysis of the results of our own research allows us to conclude that the substance G10 obtained from the plant «Zhuzgun¼ in various doses has a significant positive effect on the dynamics of biochemical parameters of blood serum in animals with experimental acute paracetamol hepatitis.The results of pathomorphological examination of the internal organs of laboratory animals (rats) with intragastric administration of substance G10 also allow us to conclude that it has a hepatoprotective effect. The results of microscopic and biochemical studies of laboratory animals (rats) in acute toxic hepatitis with a new substance from the plant Calligonum allow us to conclude that the substance G10 has a hepatoprotective property. The obtained preliminary data on the hepatoprotective efficacy of substance G10 open up new prospects for further studies of its pharmacological properties.


Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas , Extractos Vegetales , Acetaminofén/toxicidad , Animales , Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Hígado , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Ratas , Ratas Wistar
4.
Georgian Med News ; (312): 145-149, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33964843

RESUMEN

The aim of the study was to determine the stable diagnostic traits of a Caucasian endemic plant Daphne axilliflora (Keissl.) Pobed. and study its phytochemical composition. The following stable diagnostic characteristic were identified during the microstructural analysis: leaf surface glabrous, hypostomatic, dorsoventral; epidermal cells chaotic; curved with curved walls on both the upper and lower epidermis; stomata anomocytic; well visible chloroplasts, rhomboid and needle shaped crystals in lower leaf epidermis; leaf vascular bundles reverse-collateral; vascular system monocyclic, bilateral; wood diffuse-porous; xylem parenchyma is apotracheal, scanty metatracheal; vessel walls are predominantly characterized by spiral and pitted thickening; collenchyma lamellar; radial rays in single rows, heterogeneous. Phytochemical analysis showed the presence of terpene and phenolic substances, including coumarins. Two well-known coumarins (daphnin and daphnetin) were isolated and identified.


Asunto(s)
Daphne , Cumarinas , Fitoquímicos , Extractos Vegetales , Hojas de la Planta , Tallos de la Planta
5.
An Acad Bras Cienc ; 93(2): e20190366, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33950151

RESUMEN

Candida spp. are naturally opportunistic and can promote infections. These yeasts can form biofilm, after penetration and adhesion to the biotic or abiotic surfaces. Preexisting diseases, treatments with drugs and radiation therapy, medical procedures, and parafunctional habits favor the installation of a fungal infection. Increased resistance to the available antifungals has become a concern. Therefore, alternative methods to control them have been evaluated, including the use of plant substances. In this study, the antibiofilm effect of R. officinalis L. extract was analyzed on C. albicans, C. dubliniensis, C. glabrata, C. krusei, and C. tropicalis. A phytochemical analysis of the extract was performed. Biofilms were formed for 48 h and exposed to the different concentrations of the extract (50, 100, and 200 mg/mL) for 5 min or 24 h. The effect of the plant extract was compared to the antifungal nystatin. Rosmarinus officinalis L. extract was constituted of phenols and flavonoids, highlighting the presence of chlorogenic acid derivatives in its composition. Biofilm reductions were observed after exposure to the plant extract for both periods. The plant extract provided a reduction similar to the antifungal. Thus, R. officinalis L. extract showed antibiofilm effect on Candida spp. comparable to the nystatin.


Asunto(s)
Candida , Rosmarinus , Antifúngicos/farmacología , Biopelículas , Candida albicans , Pruebas de Sensibilidad Microbiana , Nistatina/farmacología , Extractos Vegetales/farmacología
6.
Molecules ; 26(7)2021 Apr 06.
Artículo en Inglés | MEDLINE | ID: mdl-33917440

RESUMEN

Periodontitis is a set of chronic inflammatory diseases caused by the accumulation of Gram-negative bacteria on teeth, resulting in gingivitis, pocket formation, alveolar bone loss, tissue destruction, and tooth loss. In this study, the contents of ginsenosides isolated from Panax ginseng fruit extract were quantitatively analyzed, and the anti-inflammatory effects were evaluated in human periodontal ligament cells. The major ginsenosides, Re, Ra8, and Rf, present in ginseng fruit were simultaneously analyzed by a validated method using high-performance liquid chromatography with a diode-array detector; Re, Ra8, and Rf content per 1 g of P. ginseng fruit extract was 1.01 ± 0.03, 0.33 ± 0.01, and 0.55 ± 0.04 mg, respectively. Ginsenosides-Re, -Ra8, and -Rf inhibited the production of pro-inflammatory factors and the expression of important cytokines in periodontitis by inducing the expression of heme oxygenase 1 (HO-1), promoting osteoblast differentiation of periodontal ligament cells, suppressing alveolar bone loss, and promoting the expression of osteoblast-specific genes, such as alp, opn, and runx2. An inhibitory effect of these ginsenosides on periodontitis and alveolar bone loss was observed via the regulation of HO-1 and subsequent epidermal growth factor receptor (EGFR) signaling. Silencing EGFR with EGFR siRNA confirmed that the effect of ginsenosides on HO-1 is mediated by EGFR. In conclusion, this study evaluated the contents of ginsenosides-Re, -Ra8, and -Rf isolated from P. ginseng fruit extract. Therefore, these results provide important basic data for future P. ginseng fruit component studies and suggest that ginsenosides Re, Ra8, and Rf have potential as future treatment options for periodontitis.


Asunto(s)
Antiinflamatorios/farmacología , Receptores ErbB/metabolismo , Ginsenósidos/aislamiento & purificación , Ginsenósidos/farmacología , Hemo-Oxigenasa 1/metabolismo , Osteogénesis/efectos de los fármacos , Panax/química , Ligamento Periodontal/citología , Diferenciación Celular/efectos de los fármacos , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Supervivencia Celular/efectos de los fármacos , Citocinas/metabolismo , Frutas/química , Regulación de la Expresión Génica/efectos de los fármacos , Ginsenósidos/química , Humanos , Mediadores de Inflamación/metabolismo , Límite de Detección , Lipopolisacáridos/farmacología , Factor 2 Relacionado con NF-E2/metabolismo , Osteoblastos/citología , Osteoblastos/efectos de los fármacos , Osteoblastos/metabolismo , Extractos Vegetales/química , Porphyromonas gingivalis/química , Análisis de Regresión , Transducción de Señal/efectos de los fármacos
7.
Molecules ; 26(7)2021 Apr 06.
Artículo en Inglés | MEDLINE | ID: mdl-33917599

RESUMEN

The Lamiaceae family is an important source of species among medicinal plants highly valued for their biological properties and numerous uses in folk medicine. Origanum is one of the main genera that belong to this family. The purpose of the study was to determine the phenolic composition of the Origanum vulgare extract and evaluate the antimicrobial, antioxidant, and inhibitory activities of this species that grows in the Andean region of the Atacama Desert. High-performance liquid chromatography was performed to determine the main phenols. Rosmarinic acid was identified as the predominant phenolic compound in this species (76.01 mg/100 g DW), followed by protocatechuic acid, which to our knowledge, no previous study reported similar concentrations in O. vulgare. The oregano extract exhibited a content of total phenolic (3948 mg GAE/100 g DW) and total flavonoid (593 mg QE/100 g DW) with a higher DPPH antioxidant activity (IC50 = 40.58 µg/mL), compared to the same species grown under other conditions. Furthermore, it was found to inhibit α-glucosidase activity with an IC50 value (7.11 mg/mL) lower than acarbose (129.32 mg/mL). Pseudomonas syringae and Pantoea agglomerans (both MIC 0.313 mg/mL and MBC 1.25 mg/mL) were the bacteria most susceptible to oregano extract with the lowest concentration necessary to inhibit bacterial growth. These results open the door for the potential use of this plant to manage chronic diseases, and they expand the knowledge of the species cultivated in arid environmental conditions.


Asunto(s)
Antibacterianos/farmacología , Antioxidantes/farmacología , Clima Desértico , Inhibidores Enzimáticos/farmacología , Origanum/química , Bacterias/efectos de los fármacos , Chile , Cromatografía Líquida de Alta Presión , Pruebas de Sensibilidad Microbiana , Fenoles/análisis , Extractos Vegetales/química
8.
Molecules ; 26(7)2021 Apr 06.
Artículo en Inglés | MEDLINE | ID: mdl-33917644

RESUMEN

Oxidative stability of food is one of the most important parameters affecting integrity and consequently nutritional properties of dietary constituents. Antioxidants are widely used to avoid deterioration during transformation, packaging, and storage of food. In this paper, novel poly (vinyl alcohol) (PVA)-based films were prepared by solvent casting method adding an hydroxytyrosol-enriched extract (HTyrE) or an oleuropein-enriched extract (OleE) in different percentages (5, 10 and 20% w/w) and a combination of both at 5% w/w. Both extracts were obtained from olive oil wastes and by-products using a sustainable process based on membrane technologies. Qualitative and quantitative analysis of each sample carried out by high performance liquid chromatography (HPLC) and nuclear resonance magnetic spectroscopy (NMR) proved that the main components were hydroxytyrosol (HTyr) and oleuropein (Ole), respectively, two well-known antioxidant bioactive compounds found in Olea europaea L. All novel formulations were characterized investigating their morphological, optical and antioxidant properties. The promising performances suggest a potential use in active food packaging to preserve oxidative-sensitive food products. Moreover, this research represents a valuable example of reuse and valorization of agro-industrial wastes and by-products according to the circular economy model.


Asunto(s)
Antioxidantes/farmacología , Glucósidos Iridoides/farmacología , Aceite de Oliva/química , Alcohol Feniletílico/análogos & derivados , Extractos Vegetales/farmacología , Alcohol Polivinílico/química , Residuos/análisis , Rastreo Diferencial de Calorimetría , Depuradores de Radicales Libres/química , Glucósidos Iridoides/química , Fenoles/análisis , Alcohol Feniletílico/química , Alcohol Feniletílico/farmacología , Espectroscopía de Protones por Resonancia Magnética , Termogravimetría
9.
J Chromatogr A ; 1643: 462080, 2021 Apr 26.
Artículo en Inglés | MEDLINE | ID: mdl-33799073

RESUMEN

Acorus tatarinowii Schott is a traditional Chinese medicine used to treat memory and cognitive dysfunction. Because of their efficacy and lower toxic effects, research on α- and ß-asarone, the phytoconstituents, has attracted attention owing to their remarkable pharmacological activities. Silver ion coordination complexation high-speed counter-current chromatography was used to separate these isomers from A. tatarinowii extract, coupled with accelerated solvent extraction. Accelerated solvent extraction parameters were investigated with single-factor and orthogonal testing. A two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (2:1:2:1, v/v) with 0.50 mol/L silver ions was selected for separation. From 2.0 g crude extract, 1.4 g of ß-asarone and 0.09 g of α-asarone were obtained with purities over 98% by sequential sample loading in 20 h. The isolated compounds were identified by electrospray ionization mass spectrometry, 1H and 13C NMR. Silver ions significantly increased the separation factor and retention of the stationary phase. The chromatographic behavior indicated that cis-configuration was more strongly complexed with the silver ion. This was further demonstrated with the help of computational analysis. In conclusion, the established method could be employed to separate other cis-trans or E/Z isomers that form coordination complexes.


Asunto(s)
Acorus/química , Anisoles/análisis , Distribución en Contracorriente/métodos , Acorus/metabolismo , Anisoles/aislamiento & purificación , Teoría Funcional de la Densidad , Isomerismo , Extracción Líquido-Líquido , Espectroscopía de Resonancia Magnética , Extractos Vegetales/química , Plata/química , Espectrometría de Masa por Ionización de Electrospray
10.
Phytochemistry ; 186: 112746, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33799191

RESUMEN

Seven undescribed compounds, namely (+)-psiflavanone A, (-)-psiflavanone A, (+)-psiflavanone B, (-)-psiflavanone B, (+)-psiketide, (-)-psiketide, and psidinone, were isolated and chirally separated from the 95%-ethanol extract of Psidium guajava leaves. (+)/(-)-Psiflavanone A and (+)/(-)-psiflavanone B are two pairs of rare enantiomeric C-benzoylated flavanones isolated from the title plant for the first time. (+)/(-)-Psiketide are a pair of enantiomeric aromatic polyketides, and psidinone is the first example of a C18 phenanthrenediquinone with an angle-type 6/6/6 tricyclic skeleton. Their structures were identified by extensive analysis of HRESIMS, UV, IR, NMR, and calculated electronic circular dichroism (ECD) data. Particularly, the structures of (+)-psiflavanone A, (-)-psiflavanone A, (+)-psiflavanone B, and psidinone were further confirmed by X-ray diffraction. Psidinone exhibited antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Mycobacterium Smegmatis mc2 155, with minimum inhibitory concentrations (MICs) of 16, 8, and 0.5 µM, respectively. These results not only highlight the chemical diversity and biological activity of P. guajava but are also expected to pave way for its further expansion to other applications in future.


Asunto(s)
Psidium , Antibacterianos/farmacología , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/farmacología , Hojas de la Planta , Staphylococcus aureus
11.
Phytochemistry ; 186: 112736, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33799193

RESUMEN

Euphorbia ebracteolata Hayata, as a traditional medicine, is widely distributed in China, Korea and Japan. In China, the dried root of this plant is named 'langdu'. It is traditionally used to treat oedema, skin ulcers, abdominal distension, cough, asthma, tuberculosis swelling and other diseases. Previous studies have found that the chemical constituents of E. ebracteolata are mainly concentrated in terpenoids, acetophenones, and flavonoids. Both extracts and pure compounds from E. ebracteolata were found to possess many pharmacological activities, such as anticancer, anti-inflammatory, antiviral, and antimicrobial effects. In addition, it was reported that E. ebracteolata shows toxicity. To provide inspiration for further in-depth studies on this plant, this review will provide a timely and systematic summary of E. ebracteolata in traditional uses, phytochemistry, pharmacology toxicology, and quality control.


Asunto(s)
Botánica , Euphorbia , Euphorbiaceae , China , Etnofarmacología , Japón , Fitoquímicos/farmacología , Fitoterapia , Extractos Vegetales/toxicidad , Control de Calidad , República de Corea
12.
J Chromatogr A ; 1644: 462105, 2021 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-33823383

RESUMEN

Licorice extract (LE) is widely used in food and medicine fields. The detection of single chemical component cannot describe its overall quality. In this paper, the content of five active components and HPLC fingerprint of LE was determined. Firstly, the dual-standard system calibration method was proposed to correct the differences between different chromatographic systems. Then, the five-dimensional profiling method (FDPM) was established to evaluate LE based on the five parameters. 52 batches of LE were divided into four categories, Cluster analysis and Orthogonal projections on the latent structure - discrimination analysis (OPLS-DA) showed that the quality and quantity of LE can be reflected by FDPM. In addition, the comparison of different chromatographic system conditions showed that FDPM can dig out the potential information and provide strategies for the monitoring and calibration of fingerprint chromatographic systems.


Asunto(s)
Glycyrrhiza/química , Extractos Vegetales/análisis , Cromatografía Líquida de Alta Presión , Análisis por Conglomerados , Análisis de los Mínimos Cuadrados , Límite de Detección , Reproducibilidad de los Resultados
13.
J Nat Prod ; 84(4): 1261-1270, 2021 04 23.
Artículo en Inglés | MEDLINE | ID: mdl-33844528

RESUMEN

The coronaviruses disease 2019 (COVID-19) caused by a novel coronavirus (SARS-CoV-2) has become a major health problem, affecting more than 50 million people with over one million deaths globally. Effective antivirals are still lacking. Here, we optimized a high-content imaging platform and the plaque assay for viral output study using the legitimate model of human lung epithelial cells, Calu-3, to determine the anti-SARS-CoV-2 activity of Andrographis paniculata extract and its major component, andrographolide. SARS-CoV-2 at 25TCID50 was able to reach the maximal infectivity of 95% in Calu-3 cells. Postinfection treatment of A. paniculata and andrographolide in SARS-CoV-2-infected Calu-3 cells significantly inhibited the production of infectious virions with an IC50 of 0.036 µg/mL and 0.034 µM, respectively, as determined by the plaque assay. The cytotoxicity profile developed over the cell line representatives of major organs, including liver (HepG2 and imHC), kidney (HK-2), intestine (Caco-2), lung (Calu-3), and brain (SH-SY5Y), showed a CC50 of >100 µg/mL for A. paniculata extract and 13.2-81.5 µM for andrographolide, respectively, corresponding to a selectivity index of over 380. In conclusion, this study provided experimental evidence in favor of A. paniculata and andrographolide for further development as a monotherapy or in combination with other effective drugs against SARS-CoV-2 infection.


Asunto(s)
Andrographis , Diterpenos/farmacología , Extractos Vegetales/farmacología , /efectos de los fármacos , Animales , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Relación Dosis-Respuesta a Droga , Células Epiteliales/virología , Humanos , Hidroxicloroquina/farmacología , Pulmón/virología
14.
J Chromatogr A ; 1644: 462128, 2021 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-33845427

RESUMEN

A simple and efficient low-cost matrix solid phase dispersion (MSPD) extraction assisted by TiO2 nanoparticles and diatomaceous earth has been developed for the extraction of phenolic compounds from grape and grape pomace wastes. Experimental conditions for MSPD extraction were optimized by a factorial design and a surface response methodology. The simultaneous identification and quantification of eight main natural polyphenols (caffeic, p-coumaric, dihydroxybenzoic and gallic acid, rutin, resveratrol, quercetin and catechin) was possible by combining MSPD and capillary liquid chromatography coupled to a diode array detection and a mass simple quadrupole analyzer (cLC-DAD-MS). Good linearity and acceptable LOD (0.05-62 µg·g-1) and LOQ (0.2-207 µg·g-1) were obtained. The quantities of extracted polyphenols were within 2.4 and 333 µg·g-1, with catechin and rutin the most abundant compounds in grape pomace and grape wastes, respectively. Furthermore, considering the prospective uses of the winery bioresidues, the extracts have been characterised in terms of bioactive properties (several antioxidant activities and bacterial inhibition against Staphylococcus aureus, Escherichia coli and Pseudomona aeruginosa) and parameters such as total polyphenol and total flavonoid content. The high antioxidant activity (IC50 5.0 ± 0.4 µg ·g-1 against DPPH radical) and antibacterial activity (2.2 ± 0.3 mg·mL-1) suggests that the methodology developed is efficient, rapid and promising for the extraction of phenolic compounds with potential application as bioactive ingredients in food and cosmetic industries.


Asunto(s)
Nanopartículas/química , Polifenoles/análisis , Extracción en Fase Sólida/métodos , Titanio/química , Vitis/química , Animales , Antioxidantes/análisis , Cromatografía Liquida , Análisis Multivariante , Extractos Vegetales/química , Análisis de Componente Principal , Estudios Prospectivos , Reproducibilidad de los Resultados , Porcinos
15.
Complement Ther Clin Pract ; 43: 101383, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33862350

RESUMEN

Pomegranate is one of the most universally studied medicinal plants for its ethnomedical history, with several studies presenting the positive outcome of its use or its extracts in managing inflammation. The objective of the present trial was to investigate the efficiency of the traditionally used 5% of pomegranate peel extract in treating gingival inflammation. Herein, 34 chronic gingivitis patients were randomized in a 1:1 ratio for four weeks in a controlled, double-blind clinical trial to evaluate the effect of the adjunctive use of a pulsating jet irrigator containing 5% pomegranate peel extract solution to nonsurgical periodontal therapy against a placebo in managing these patients' condition. No adverse reactions had been reported, and within the limits of this study, it may be concluded that pomegranate peel extract can serve as a promising alternative in managing chronic gingivitis. This trial is registered on the German clinical trials register (DRKS-ID: DRKS00010602).


Asunto(s)
Gingivitis , Lythraceae , Granada (Fruta) , Frutas , Gingivitis/tratamiento farmacológico , Humanos , Extractos Vegetales/uso terapéutico
16.
Medicine (Baltimore) ; 100(17): e25613, 2021 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-33907114

RESUMEN

BACKGROUND: Diabetic nephropathy (DN) is one of the most common complications of diabetes and the main cause of kidney failure in developed countries. Clinically, DN is usually treated by controlling blood sugar and blood pressure. According to reports, the application of angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor antagonist can only target a certain stage of disease development. However, the application of Suoquan Pill (SQP) in traditional Chinese medicine has produced obvious clinical effects and minor side effects. It is used to treat DN and other diseases, but there is no systematic review of SQP in the treatment of diabetic nephropathy. This article reviews the effectiveness and safety of SQP in the treatment of DN. METHODS: The database sets the registration date for randomized controlled trials (RCT) to March 25, 2021. By searching the following eight Chinese and English electronic databases: Cochrane Library, Embase, PubMed, Science Net, China National Knowledge Infrastructure, China Biomedical Literature Database. Chinese scientific journal database and Wanfang database for analysis. The main results are clinical efficacy, urinary albumin excretion rate, symptom score and quality of life. Finally, Stata 15 was used for meta-analysis. RESULTS: This study will provide the latest evidence for SQP in the treatment of DN in the following aspects: clinical efficacy, urinary albumin excretion rate, quality of life, symptom score. CONCLUSION: The results of this study will provide evidence for evaluating the effectiveness of SQP in the treatment of DN. OSF REGISTRATION NUMBER: 10.17605/OSF.IO/KZ9RA.


Asunto(s)
Nefropatías Diabéticas/tratamiento farmacológico , Medicamentos Herbarios Chinos/uso terapéutico , Lindera , Extractos Vegetales , Humanos , Metaanálisis como Asunto , Ensayos Clínicos Controlados Aleatorios como Asunto , Proyectos de Investigación , Revisiones Sistemáticas como Asunto , Resultado del Tratamiento
17.
Molecules ; 26(6)2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33804083

RESUMEN

Alkaloids of the Lycopodiaceae family are of great interest to researchers due to their numerous properties and wide applications in medicine. They play a very important role mainly due to their potent antioxidant, antidepressant effects and a reversible ability to inhibit acetylcholinesterase (AChE) enzyme activity. This property is of immense importance due to the growing problem of an increasing number of patients with neurodegenerative diseases in developed countries and a lack of effective and efficient treatment for them. Numerous studies have shown that Lycopodiaceae alkaloids are a rich source of AChE inhibitors. In the obtaining of new therapeutic phytochemicals from plant material, the extraction process and its efficiency is crucial. Therefore, the aim of this work was to optimize the conditions of modern PLE to obtain bioactive alkaloids from two Lycopodium species: L. clavatum L. and L. annotinum L. Five different solvents of different polarity were used for prepared plant extracts in order to compare the alkaloid content in and thereby effectiveness of the entire extraction. PLE parameters were used based on multiple studies conducted that gave the highest alkaloids recovery. Crude extracts were purified using solid-phase extraction (SPE) on Oasis HLB cartridge and examined by HPLC/ESI-QTOF-MS of the highly abundant alkaloids. To the best of our knowledge, this is the first time such high recoveries have been obtained for known Lycopodiaceae alkaloids. The best extraction results of alkaloid-lycopodine were detected in the dichloromethane extract from L. clavatum, where the yield exceeded 45%. The high recovery of annotinine above 40% presented in L. annotinum was noticed in dichloromethane and ethyl acetate extracts. Moreover, chromatograms were obtained with all isolated alkaloids and the best separation and quality of the bands in methanolic extracts. Interestingly, no alkaloid amounts were detected in cyclohexane extracts belonging to the non-polar solvent. These results could be helpful for understanding and optimizing the best conditions for isolating potent AChE inhibitors.


Asunto(s)
Alcaloides/química , Lycopodiaceae/química , Lycopodium/química , Extractos Vegetales/química , Acetilcolinesterasa/química , Antioxidantes/química , Inhibidores de la Colinesterasa/química , Cromatografía Líquida de Alta Presión/métodos , Espectrometría de Masas/métodos , Extracción en Fase Sólida/métodos
18.
Molecules ; 26(6)2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33804141

RESUMEN

In this work, a green extraction technique, subcritical water extraction (SBWE), was employed to extract active pharmaceutical ingredients (APIs) from an important Chinese medicinal herb, Salvia miltiorrhiza (danshen), at various temperatures. The APIs included tanshinone I, tanshinone IIA, protocatechualdehyde, caffeic acid, and ferulic acid. Traditional herbal decoction (THD) of Salvia miltiorrhiza was also carried out for comparison purposes. Reproduction assay of herbal extracts obtained by both SBWE and THD were then conducted on Caenorhabditis elegans so that SBWE conditions could be optimized for the purpose of developing efficacious herbal medicine from Salvia miltiorrhiza. The extraction efficiency was mostly enhanced with increasing extraction temperature. The quantity of tanshinone I in the herbal extract obtained by SBWE at 150 °C was 370-fold higher than that achieved by THD extraction. Reproduction evaluation revealed that the worm reproduction rate decreased and the reproduction inhibition rate increased with elevated SBWE temperatures. Most importantly, the reproduction inhibition rate of the SBWE herbal extracts obtained at all four temperatures investigated was higher than that of traditional herbal decoction extracts. The results of this work show that there are several benefits of subcritical water extraction of medicinal herbs over other existing herbal medicine preparation techniques. Compared to THD, the thousand-year-old and yet still popular herbal preparation method used in herbal medicine, subcritical water extraction is conducted in a closed system where no loss of volatile active pharmaceutical ingredients occurs, although analyte degradation may happen at higher temperatures. Temperature optimization in SBWE makes it possible to be more efficient in extracting APIs from medicinal herbs than the THD method. Compared to other industrial processes of producing herbal medicine, subcritical water extraction eliminates toxic organic solvents. Thus, subcritical water extraction is not only environmentally friendly but also produces safer herbal medicine for patients.


Asunto(s)
Extractos Vegetales/química , Salvia miltiorrhiza/química , Agua/química , Abietanos/química , Benzaldehídos/química , Ácidos Cafeicos/química , Catecoles/química , Ácidos Cumáricos/química , Medicamentos Herbarios Chinos/química , Calor , Plantas Medicinales/química
19.
Molecules ; 26(6)2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33804199

RESUMEN

Neuropathic pain is described as the "most terrible of all tortures that a nerve wound may inflict." The aim of the present study was to demonstrate the antinociceptive effect of Symplocos chinensis f. pilosa Ohwi water extract (SCW) and synthesized derivatives of the isolated compound. The antinociceptive effect was tested using the acetic acid-induced writhing and 5% formalin tests. Antinociceptive effects on neuropathic pain were evaluated using the von Frey test with chronic constriction injury (CCI) and surgical nerve injury (SNI) models and tail-flick test with a vincristine-induced pain model. An Ames test was also conducted. 5-hydroxymethylfurfural (5-HMF) was isolated and derivatives were synthesized with various acid groups. Among the plant water extracts, SCW showed significantly effective activity. Additionally, SCW presented antinociceptive effects in the neuropathic pain models. The SCW water fraction resulted in fewer writhes than the other fractions, and isolated 5-HMF was identified as an effective compound. Because 5-HMF revealed a positive response in the Ames test, derivatives were synthesized. Among the synthesized derivations, 5-succinoxymethylfurfural (5-SMF) showed the best effect in the neuropathic pain model. Our data suggest that SCW and the synthesized compound, 5-SMF, possess effective antinociceptive activity against neuropathic pain.


Asunto(s)
Ericales/química , Neuralgia/tratamiento farmacológico , Extractos Vegetales/farmacología , Analgésicos/farmacología , Animales , Modelos Animales de Enfermedad , Masculino , Ratones , Ratones Endogámicos ICR , Nervio Ciático/efectos de los fármacos
20.
Molecules ; 26(6)2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33804179

RESUMEN

Amomum tsao-ko Crevost et Lemaire (Zingiberaceae) is a medicinal herb found in Southeast Asia that is used for the treatment of malaria, abdominal pain, dyspepsia, etc. The aim of this study was to investigate the effect of an ethanol extract of Amomum tsao-ko (EAT) on obesity and hyperlipidemia in C57BL/6 mice fed a high-carbohydrate diet (HCD). First, the mice were divided into five groups (n = 6/group) as follows: normal diet, HCD, and HCD+EAT (100, 200, and 400 mg/kg/day), which were orally administered with EAT daily for 84 days. Using microcomputed tomography (micro-CT) analysis, we found that EAT inhibited not only body-weight gain, but also visceral fat and subcutaneous fat accumulation. Histological analysis confirmed that EAT decreased the size of fat tissues. EAT consistently improved various indices, including plasma levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein, high-density lipoprotein, atherogenic index, and cardiac risk factors, which are related to dyslipidemia-a major risk factor for heart disease. The contents of TC and TG, as well as the lipid droplets of HCD-induced hepatic accumulation in the liver tissue, were suppressed by EAT. Taken together, these findings suggest the possibility of developing EAT as a therapeutic agent for improving HCD-induced obesity and hyperlipidemia.


Asunto(s)
Amomum/química , Carbohidratos/efectos adversos , Dislipidemias/tratamiento farmacológico , Obesidad/tratamiento farmacológico , Plantas Medicinales/química , Zingiberaceae/química , Tejido Adiposo/efectos de los fármacos , Animales , Dieta/efectos adversos , Dislipidemias/metabolismo , Lipoproteínas LDL/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Ratones , Ratones Endogámicos C57BL , Obesidad/metabolismo , Extractos Vegetales/química , Extractos Vegetales/farmacología , Triglicéridos/metabolismo
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